Browse publications by year: 2025

  1. Ullah N, Shah Z, Jan R, Vrinceanu N, Farhan M, Antonescu E
    Sci Rep, 2025 Feb 20;15(1):6262.
    PMID: 39979382 DOI: 10.1038/s41598-025-90182-1
    Vector-borne infections impose a significant burden on global health systems and economies due to their widespread impact and the substantial resources required for prevention, control, and treatment efforts. In this work, we formulate a mathematical model for the transmission dynamics of a vector-borne infection with the effect of vaccination through the Atangana-Baleanu derivative. The solutions of the model are positive and bounded for positive initial values of the state variable. We presented the basic concept and theory of fractional calculus for the analysis of the model. We determine the threshold parameter, denoted by [Formula: see text], using the next-generation matrix method. The local asymptotic stability of the system at the disease-free equilibrium is analyzed. To establish the existence of solutions for the proposed model, we employ fixed-point theory. A numerical scheme is developed to visualize the system's dynamical behavior under varying input parameters. Numerical simulations are conducted to illustrate how these parameters influence the dynamics of the system. The results highlight key factors affecting the transmission and control of vector-borne diseases, offering insights into strategies for prevention and mitigation.
    MeSH terms: Animals; Computer Simulation; Humans; Models, Biological; Models, Theoretical
  2. Salari N, Beiromvand M, Abdollahi R, Hemmati M, Heidarian P, Hashemian K, et al.
    Arch Gynecol Obstet, 2025 Feb 20.
    PMID: 39979469 DOI: 10.1007/s00404-025-07982-z
    BACKGROUND: Evidence shows that twin pregnancy is associated with increased adverse outcomes, including stillbirth. The present study aimed to investigate the global prevalence of stillbirth among fetuses resulting from twin pregnancies through a systematic review and meta-analysis.

    METHODS: To identify relevant studies, we adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. We searched databases including PubMed, Web of Science, Google Scholar, Scopus, Embase, and Science Direct using keywords such as "stillbirth," "twin pregnancy," "fetus," and "prevalence" without applying any time constraints to the search. The quality of the articles was evaluated using the STROBE checklist. To ensure the reliability of our findings, we employed a random-effects model for analysis, and the heterogeneity of the studies was assessed using the I2 index. Data analysis was conducted using Version 2 of Comprehensive Meta-Analysis software.

    RESULTS: In a review of 10 studies with a sample size of 627,797 people, our meta-analysis revealed a global prevalence of stillbirth among fetuses resulting from twin pregnancies, reported to be 1.4% (95% CI 0.9-2.1). Our study also showed that with increasing sample size and years of study, the global prevalence of stillbirth among fetuses resulting from twin pregnancies decreased (p 

  3. Hong WQ, Lee WH, Musa SH, Kamaruzaman NA, Loo CY
    Biometals, 2025 Feb 20.
    PMID: 39979667 DOI: 10.1007/s10534-025-00670-0
    Chronic wound healing is associated with prolonged elevated inflammation and high levels of oxidative stress leading to cell death. The majority of wounds are colonized with antibiotic-resistant bacterial biofilms such as Pseudomonas aeruginosa and Staphylococcus aureus. An ideal wound treatment should include agents with antioxidant, anti-inflammatory, and antibiofilm behavior. Therefore, in this study, a combination of curcumin nanoparticle (Cur-NP) and silver nanoparticle (AgNP) (Cur-NP/AgNP) loaded PVA hydrogel was used to inhibit the bacterial attachment and subsequent biofilm formation of P. aeruginosa and S. aureus. Cur was known for its antioxidant and anti-inflammatory effect while being non-toxic to cells. Meanwhile, AgNP demonstrated superior anti-bacterial and antibiofilm activities against both P. aeruginosa and S. aureus. Cur-NP/AgNP loaded PVA hydrogels completely inhibited the bacterial attachment and biofilm formation, possibly due to synergistic effect of Cur-NPs and AgNPs in killing the bacterial cells. It should be highlighted that no surviving bacterial cells were noted for Cur-NP/AgNP loaded hydrogels. On the other hand, AgNPs or Cur-NPs alone loaded hydrogels were unable to achieve complete inhibition of biofilm formation, even though significant reduction in the biofilm mass was noted compared with control samples. Cur-NP and AgNP exerted oxidative-stress induced cell death in HaCaT cells via mitochondrial dysfunction, mitochondrial membrane potential (MMP) reduction, adenosine triphosphate inhibition, and increased cytochrome C release. The toxicity of formulation followed the decreasing trend: Cur-NP/AgNP 
  4. Xu X, Hanafi Z, Gao L
    BMC Psychol, 2025 Feb 20;13(1):145.
    PMID: 39980053 DOI: 10.1186/s40359-025-02464-y
    BACKGROUND: The impact of mothers' socioeconomic status (SES) on late adolescents' emotional stability, as well as the mechanisms underlying this relationship, remain poorly understood in China. Additionally, the mechanisms by which SES impacts emotional stability may need investigated separately for the male and female adolescents.

    METHOD: This study conducted a snowball sampling and invited undergraduates to complete a survey via online. A sample of 445 Chinese undergraduate students (229 males, age range of 18-25 years) completed questionnaires concerning their mothers' monthly income and educational levels, emotional stability, and maternal parenting styles. Independent samples t-test, correlation analyses and regression analyses were performed.

    RESULTS: The findings suggested the levels of emotional stability in female students were significantly lower than those of male students. Mothers' SES was related to late adolescents' emotional stability significantly. Moreover, maternal parenting styles (emotional warmth, punishment, overprotection, and rejection) significantly mediated the relationship between mothers' SES and late adolescents' emotional stability. Additionally, the particular features of these relationships varied according to the sex of the late adolescents. For the male students, maternal parenting styles could not significantly serve as mediating roles. For the female adolescents, the effect of maternal SES on emotional stability was partially mediated by four separate pathways: (1) maternal emotional warmth, (2) maternal punishment, (3) maternal overprotection, and (4) maternal rejection. These findings provide crucial practical implications for identification, prevention, and intervention efforts in late adolescents' emotional stability across sex.

    CONCLUSION: This study sheds light on the relationship between mothers' SES and late adolescents' emotional stability, and the indirect effects of maternal emotional warmth, punishment, overprotection, and rejection serving as mediating roles. Maternal parenting styles had a higher effect on the emotional stability in female adolescents than male adolescents. This also provides crucial practical implications for identifying, preventing, and intervening in late adolescent emotional stability, which may differ between female and male adolescents.

    MeSH terms: Adolescent; Adult; China; Emotions; Female; Humans; Male; Surveys and Questionnaires; Sex Factors; Social Class; Students/psychology; Students/statistics & numerical data; Young Adult
  5. Feng G, Targher G, Byrne CD, Yilmaz Y, Wai-Sun Wong V, Adithya Lesmana CR, et al.
    JHEP Rep, 2025 Mar;7(3):101271.
    PMID: 39980749 DOI: 10.1016/j.jhepr.2024.101271
    BACKGROUND & AIMS: This study used the Global Burden of Disease data (2010-2021) to analyze the rates and trends of point prevalence, annual incidence, and years lived with disability (YLDs) for metabolic dysfunction-associated steatotic liver disease (MASLD) in 204 countries.

    METHODS: Total numbers and age-standardized rates per 100,000 population for MASLD prevalence, annual incidence, and YLDs were compared across regions and countries by age, sex, and sociodemographic index (SDI). Smoothing spline models were used to evaluate the relationship between the burden of MASLD and SDI. Estimates were reported with uncertainty intervals (UI).

    RESULTS: Globally, in 2021, the age-standardized rates per 100,000 population of point prevalence of MASLD were 15,018.1 cases (95% UI 13,756.5-16,361.4), annual incidence rates were 608.5 cases (598.8-617.7), and YLDs were 0.5 (0.3-0.8) years. MASLD point prevalence was higher in men than women (15,731.4 vs. 14,310.6 cases per 100,000 population). Prevalence peaked at ages 45-49 for men and 50-54 for women. Kuwait (32,312.2 cases per 100,000 people; 95% UI: 29,947.1-34,839.0), Egypt (31,668.8 cases per 100,000 people; 95% UI: 29,272.5-34,224.7), and Qatar (31,327.5 cases per 100,000 people; 95% UI: 29,078.5-33,790.9) had the highest prevalence rates in 2021. The largest increases in age-standardized point prevalence estimates from 2010 to 2021 were in China (16.9%, 95% UI 14.7%-18.9%), Sudan (13.3%, 95% UI 9.8%-16.7%) and India (13.2%, 95% UI 12.0%-14.4%). MASLD incidence varied with SDI, peaking at moderate SDI levels.

    CONCLUSIONS: MASLD is a global health concern, with the highest prevalence reported in Kuwait, Egypt, and Qatar. Raising awareness about risk factors and prevention is essential in every country, especially in China, Sudan and India, where disease incidence and prevalence are rapidly increasing.

    IMPACT AND IMPLICATIONS: This research provides a comprehensive analysis of the global burden of MASLD, highlighting its rising prevalence and incidence, particularly in countries with varying sociodemographic indices. The findings are significant for both clinicians and policymakers, as they offer critical insights into the regional disparities in MASLD burden, which can inform targeted prevention and intervention strategies. However, the study's reliance on modeling and available data suggests cautious interpretation, and further research is needed to validate these findings in clinical and real-world settings.

  6. Khandaker MU, Osman H, Issa SAM, Uddin MM, Ullah MH, Wahbi H, et al.
    RSC Adv, 2025 Feb 19;15(8):5766-5780.
    PMID: 39980992 DOI: 10.1039/d4ra09093d
    The research examines the exceptional physical characteristics of Mg3AB3 (A = N, Bi; B = F, Br, I) perovskite compounds through density functional theory to assess their feasibility for photovoltaic applications. Mechanical characterization further supports their stability where out of all the compounds, Mg3BiI3 demonstrates high ductility, while Mg3NF3 and Mg3BiBr3 possess a brittle nature. The calculated elastic constants and anisotropy factors also substantiate their mechanical stability, while there is an observed declining trend in Debye temperature with increase in atomic number. From the electronic point of view, Mg3NF3 can be considered as a wide-bandgap insulator with the bandgap of 6.789 eV, whereas Mg3BiBr3 and Mg3BiI3 can be classified as semiconductors suitable for photovoltaic applications bandgaps of 1.626 eV and 0.867 eV, respectively. The optical characteristics of such materials are excellent and pronounced by high absorption coefficients, low reflectivity, and good dielectrics, which are very important in the collection of solar energy. Among them, Mg3BiBr3 and Mg3BiI3 possess high light absorption coefficient, moderate reflectivity, and good electrical conductivity, indicating that they are quite suitable for applying the photoelectric conversion materials for solar cells. In addition, thermal analysis shows that Mg3NF3 is a good heat sink material, Mg3BiBr3 and Mg3BiI3 are favorable for thermal barrier coating materials. Due to their high absorption coefficients, low reflectance and suitable conductivity, both Mg3BiBr3 and Mg3BiI3 could be regarded as the most appropriate materials for the creation of the next generation of photovoltaic converters.
  7. Arshad M, Onn CW, Ahmad A, Mogwe G
    Front Big Data, 2025;8:1513027.
    PMID: 39981020 DOI: 10.3389/fdata.2025.1513027
    As the trend in the current generation with the use of mobile devices is rapidly increasing, online video streaming has risen to the top in the entertainment industry. These platforms have experienced radical expansion due to the incorporation of Big Data Analytics and Artificial Intelligence which are critical in improving the user interface, improving its functioning, and customization of recommended content. This paper seeks to examine how Big Data Analytics makes it possible to obtain large amounts of data about users and how they view, what they like, or how they behave. While customers benefit from this data by receiving more suitable material, getting better recommendations, and allowing for more efficient content delivery, AI utilizes it. As a result, the study also points to the importance and relevance of such technologies to promote business development, and user interaction and maintain competitiveness in the online video streaming market with examples of their effective application. This work presents a comprehensive investigation of the combined role of Big Data and AI and presents the necessary findings to determine their efficacy as success factors of existing and future video streaming services.
  8. Tani N, Ng CH, Lee SL, Lee CT, Muhammad N, Kondo T, et al.
    Front Genet, 2025;16:1552024.
    PMID: 39981260 DOI: 10.3389/fgene.2025.1552024
    INTRODUCTION: The role of syngameons in adaption to microgeographical environmental heterogeneity is important and could be one of the sources of rich species diversity in tropical forests. In addition, negative frequency- or density-dependent selection is one of the major processes contributing to the maintenance of genetic diversity.

    METHODS: To assess genetic factors that affect the fitness of seedlings of Rubroshorea curtisii, a dominant canopy tree species in hill dipterocarp forests, the inter- and intra-population genetic structure of individuals from natural populations and individuals at two permanent plots in a hill dipterocarp forest with reproductive stage was studied. Further, a total of 460 seedlings derived from six mother trees in the plot were raised in a nursery, and their pollen donors were identified using genetic marker based paternity assignment. Seed weight, bi-parental genetic relatedness, and bi-parental genetic heterogeneity based on the clustering analysis were used to analyze their effects on seedling fitness.

    RESULTS: A Bayesian based clustering analysis revealed that three genetically distinct clusters were observed in almost all populations throughout the distributional range of the species in Malay Peninsula and provided the optimum explanation for the genetic structure of 182 mature individuals in the plots. The two clusters showed larger genetic differentiation from the ancestral admixture population, but the other one was not differentiated. The bi-parental larger genetic heterogeneity was associated with a significantly higher probability of seedling survivorship, and likewise, higher performance of vertical growth of the seedlings; but the seed weight and genetic relatedness did not significantly affect those.

    DISCUSSION: This evidence suggests that fitter seedlings derived from mating between parents with different genetic clusters contribute to maintaining genetic diversity through negative frequency-dependent selection and may have an important role in adaptation in the tropical forest plant community.

  9. Ullah S, Umer MF, Chandran SP
    Eur J Ophthalmol, 2025 Feb 21.
    PMID: 39981627 DOI: 10.1177/11206721251314541
    INTRODUCTION: Myopia is a refractive error where distant objects are not clearly seen and appear blurred. Goal of this study was to evaluate long-term effectiveness of Repeated Low-Level Red Light (RLRL) therapy for children with myopia, which primarily focused on Axial length (AL) and Spherical Equivalent Refraction (SER) as primary endpoints.

    METHODS: The research compared outcomes between RLRL treatment with Single Vision Spectacles (SVS) for childhood myopia management. We performed a systematic literature search in the PubMed, Embase and Cochrane databases using "Myopia" and " Repeated Low-Level Red Light ". Mean differences (MD) were estimated and the effects of therapies measured. Publication bias and heterogeneity analysis were carried out by Inverted Precision Effect Test-Precision Effect Estimate Standard Error (PET-PEESE) (and subsequent Search Sequential Analysis) and Tau test. Bayesian meta-analysis was performed using Jaffrey Amazing Statistical Package (JASP).

    RESULTS: This meta-analysis comprised 1,714 participants: 824 in RLRL and 890 in SVS group. Pooled effect size for AL reduction was 0.953 ± 0.294, (95% credible interval (CI) 0.775 to 0.980). Pooled effect size for SER reduction was 1.521 ± 0.662 (95% CI 0.102 to 2.736). PET-PEESE analysis revealed no significant publication bias (p-value 0.407). Random effects models were employed for presence of significant heterogeneity (3.9 and 5.7 for AL; 5.7 for SER), alongside degree of variation (0.828 & 1.665) for Tau (τ), which estimates the between-study variance.

    CONCLUSION: Long-term observations indicate that RLRL treatment significantly influences myopia management, leading to considerable reductions in both AL and SER. Additional research is essential to investigate potential long-term rebound effects.

  10. Bustos AY, Taranto MP, Gerez CL, Agriopoulou S, Smaoui S, Varzakas T, et al.
    Probiotics Antimicrob Proteins, 2025 Feb;17(1):138-158.
    PMID: 38829565 DOI: 10.1007/s12602-024-10273-9
    In recent years, more and more scientific community, food producers, and food industry show increased interest in functional foods containing probiotics, which is a big challenge. The consumption of probiotics in the context of a balanced diet through the consumption of functional foods or through the intake of pharmaceutical preparations has proven to contribute to the improvement of human health, even contributing to the prevention of diseases. In order for probiotics to be considered suitable for consumption, they must contain a minimum concentration of viable cells, namely, at least 107 colony forming units of beneficial microbes per gram. Ensuring the viability of bacterial cells until the moment of consumption is the overriding priority of functional probiotic food manufacturers. Probiotic bacteria are subject to stress conditions not only during food manufacturing but also during gastrointestinal passage, which limit or even compromise their functionality. This paper first examines all the stressful conditions faced by probiotic cells in their production stages and related to the conditions present in the bioreactor fermentation and drying processes as well as factors related to the food matrix and storage. The stress situations faced by probiotic microorganisms during the gastrointestinal transit especially during stomach and intestinal residence are also analyzed. In order to understand the adaptation mechanisms of probiotic bacteria to gastrointestinal stress, intrinsic and adaptive mechanisms identified in probiotic strains in response to acid stress and to bile and bile acid stress are analyzed. In addition, improvement strategies for multiple stress tolerance of lactic acid bacteria through directions dealing with stress, accumulation of metabolites, use of protectants, and regulation of technological parameters are examined. Finally, the definition of postbiotics, inanimate microorganisms and/or their components conferring health benefits, is also introduced. Postbiotics include cell lysates, enzymes, and cell wall fragments derived from probiotic bacteria and may represent an alternative to the use of probiotics, when they do not tolerate stressful conditions.
    MeSH terms: Bacteria/metabolism; Fermentation; Food Microbiology; Humans; Stress, Physiological*; Gastrointestinal Tract/microbiology; Gastrointestinal Tract/physiology; Functional Food*
  11. Yazib SA, Choo MM, Khaliddin N, Ong CP, Choo YM, Kamar AA, et al.
    Indian J Ophthalmol, 2025 Jan 01;73(Suppl 1):S139-S143.
    PMID: 39446856 DOI: 10.4103/IJO.IJO_515_24
    PURPOSE: Prematurity has been known to trigger several cellular pathways, leading to the clinical occurrence of retinopathy of prematurity (ROP). This study compared the levels of a panel of serum cytokines in premature infants with and without ROP.

    METHODS: This is a prospective observational study. Premature infants at 36-38 weeks' gestational age were recruited, their clinical data recorded, and serum samples collected and assayed for 18 cytokines. Based on follow-up examinations, patients were divided into two groups: No ROP and ROP. The ROP group was further divided into two subgroups: non-vision-threatening ROP (non-VTROP), and vision-threatening ROP (VTROP).

    RESULTS: On univariate analysis, among the clinical parameters, gestation age, birth weight, duration of invasive ventilation, and duration of stay in neonatal intensive care unit (NICU) were found to be significant. The univariate analysis also showed an association between raised levels of VEGF-D and IL-8 in the VTROP group. Multiple logistic regression indicated that gestation age was a significant risk factor across all subgroups. Additionally, VEGF-D levels were found to be significantly associated with VTROP.

    CONCLUSION: Higher VEGF-D levels are associated with an increased risk of severe ROP that requires treatment and could potentially be used as a biomarker.

    MeSH terms: Birth Weight; Female; Follow-Up Studies; Gestational Age*; Humans; Infant, Newborn; Infant, Premature; Male; Prospective Studies; Risk Factors
  12. EMPA-KIDNEY Collaborative Group, Herrington WG, Staplin N, Agrawal N, Wanner C, Green JB, et al.
    N Engl J Med, 2025 Feb 20;392(8):777-787.
    PMID: 39453837 DOI: 10.1056/NEJMoa2409183
    BACKGROUND: In the EMPA-KIDNEY trial, empagliflozin, a sodium-glucose cotransporter 2 (SGLT2) inhibitor, had positive cardiorenal effects in patients with chronic kidney disease who were at risk for disease progression. Post-trial follow-up was designed to assess how the effects of empagliflozin would evolve after the discontinuation of the trial drug.

    METHODS: In the active trial, patients with chronic kidney disease were randomly assigned to receive either empagliflozin (10 mg once daily) or matching placebo and were followed for a median of 2 years. All the patients had an estimated glomerular filtration rate (eGFR) of at least 20 but less than 45 ml per minute per 1.73 m2 of body-surface area or an eGFR of at least 45 but less than 90 ml per minute per 1.73 m2 with a urinary albumin-to-creatinine ratio (with albumin measured in milligrams and creatinine measured in grams) of at least 200. Subsequently, surviving patients who consented were observed for 2 additional years. No trial empagliflozin or placebo was administered during the post-trial period, but local practitioners could prescribe open-label SGLT2 inhibitors, including open-label empagliflozin. The primary composite outcome was kidney disease progression or cardiovascular death as assessed from the start of the active-trial period to the end of the post-trial period.

    RESULTS: Of the 6609 patients who had undergone randomization in the active trial, 4891 (74%) were enrolled in the post-trial period. During this period, the use of open-label SGLT2 inhibitors was similar in the two groups (43% in the empagliflozin group and 40% in the placebo group). During the combined active- and post-trial periods, a primary-outcome event occurred in 865 of 3304 patients (26.2%) in the empagliflozin group and in 1001 of 3305 patients (30.3%) in the placebo group (hazard ratio, 0.79; 95% confidence interval [CI], 0.72 to 0.87). During the post-trial period only, the hazard ratio for a primary-outcome event was 0.87 (95% CI, 0.76 to 0.99). During the combined periods, the risk of kidney disease progression was 23.5% in the empagliflozin group and 27.1% in the placebo group; the risk of the composite of death or end-stage kidney disease was 16.9% and 19.6%, respectively; and the risk of cardiovascular death was 3.8% and 4.9%, respectively. There was no effect of empagliflozin on death from noncardiovascular causes (5.3% in both groups).

    CONCLUSIONS: In a broad range of patients with chronic kidney disease at risk for progression, empagliflozin continued to have additional cardiorenal benefits for up to 12 months after it was discontinued. (Funded by Boehringer Ingelheim and others; EMPA-KIDNEY ClinicalTrials.gov number, NCT03594110; EuDRACT number, 2017-002971-24.).

    MeSH terms: Aged; Cardiovascular Diseases/mortality; Cardiovascular Diseases/prevention & control; Diabetes Mellitus, Type 2/complications; Diabetes Mellitus, Type 2/drug therapy; Double-Blind Method; Female; Follow-Up Studies; Humans; Male; Middle Aged; Disease Progression*; Kaplan-Meier Estimate
  13. Mahazer MKJ, Leong SS, Wong JHD, Rozalli FI, Yahya F, Tee YC, et al.
    Br J Radiol, 2025 Mar 01;98(1167):469-474.
    PMID: 39724309 DOI: 10.1093/bjr/tqae250
    OBJECTIVES: This study explores the correlation between volunteer demographics with enthesis stiffness and intra- and interobserver agreements using shear wave elastography (SWE).

    METHODS: Ninety-eight healthy volunteers were recruited. SWE was performed on quadriceps, suprapatellar, infrapatellar, and Achilles entheses. The stiffness measurements were measured in shear wave velocity (SWV).

    RESULTS: Mann-Whitney test revealed insignificant difference in SWV among gender. Wilcoxon signed-rank test showed significant difference in SWV between left and right Achilles entheses. Spearman correlation test revealed moderate negative correlation between SWV and age group in quadriceps (ρ, -0.45, P 

    MeSH terms: Adult; Age Factors; Aged; Female; Humans; Male; Middle Aged; Reference Values; Reproducibility of Results; Observer Variation; Quadriceps Muscle/physiology; Young Adult; Healthy Volunteers*
  14. Ali M
    Curr Pharm Teach Learn, 2025 Mar;17(3):102274.
    PMID: 39724747 DOI: 10.1016/j.cptl.2024.102274
    The integration of artificial intelligence (AI) into pharmacy education offers transformative opportunities but also introduces significant challenges. This commentary explores whether AI will reshape or deform pharmacy education by analyzing its effects on personalized learning, complex concept comprehension, simulation-based clinical training, interprofessional education, and administrative efficiency. While AI-driven tools provide adaptive learning experiences, immersive visualizations, and streamlined administrative processes, concerns persist about overreliance on technology, skill atrophy, ethical and legal challenges, erosion of humanistic skills, inequities stemming from the digital divide, and faculty preparedness. To address these risks while harnessing AI's potential, a balanced approach is essential. Key strategies include integrating AI into curricula alongside traditional teaching methods, fostering digital literacy and critical thinking, enhancing humanistic education, supporting faculty development, ensuring equitable access, and establishing ethical frameworks. By thoughtfully implementing these strategies, pharmacy educators can prepare students to thrive in an AI-driven healthcare landscape while preserving core professional competencies.
    MeSH terms: Humans
  15. Lee SY, Lee YJ, Chiu CK, Chandirasegaran S, Hasan MS, Chan CYW, et al.
    Global Spine J, 2025 Feb 17.
    PMID: 39960040 DOI: 10.1177/21925682251321480
    STUDY DESIGN: Retrospective cohort study.

    OBJECTIVE: To investigate the independent predictive factors for moderate-severe pulmonary impairment (MSPI) among adolescent idiopathic scoliosis (AIS) patients.

    METHODS: The preoperative pulmonary function tests (PFTs) of 102 AIS patients (major Cobb angle ≥45°) operated between 2015 and 2020 were retrospectively reviewed. Patients were divided into 2 groups based on the predicted forced vital capacity (FVC) and forced expiratory volume in one second (FEV1), respectively. Group 1 had normal/ mild pulmonary impairment (FVC or FEV1 >65% predicted) whereas Group 2 had MSPI (FVC or FEV1 ≤65% predicted). Multivariate logistic regression analysis was performed to determine the predictive factors for MSPI.

    RESULTS: 41.2% (N = 42) and 52.0% (N = 53) of the total patients were categorised into Group 2 (MSPI) based on predicted FVC and FEV1, respectively. In general, Group 2 had more patients with a major main thoracic (MT) curve, larger MT curve with lower MT flexibility, a larger MT apical vertebra translation (MT-AVT), and a smaller thoracolumbar-lumbar (TL/L) AVT than Group 1 (P < .05). When analyzing the MT Cobb angle for every 10° increment, patients with MT Cobb angle ≥70° had MSPI (N = 31.4%). Body mass index (BMI) (FVC: aOR .8; FEV1: aOR .9) and MT Cobb angle (for every 10° increment, FVC: aOR 1.7; FEV1: aOR 1.8) were the significant independent predictive factors for MSPI.

    CONCLUSION: MSPI was evident in patients with MT Cobb angle ≥70°, with MT Cobb angle and BMI being the significant independent predictive factors.

  16. Sa'aid N, Tan JS
    PMID: 39968919 DOI: 10.1080/10826068.2025.2467441
    In recent years, the demand for probiotic beverages has surged, with dairy products traditionally serving as the primary sources of probiotics. However, many consumers face health issues such as lactose intolerance, milk allergies, and high cholesterol, which prevent them from consuming dairy products. This has led to the exploration of nondairy alternatives, particularly fruit juices, as carriers for probiotics. Lactic acid bacteria (LAB) have been identified as beneficial probiotics that can be incorporated into these beverages. The inclusion of prebiotics, such as inulin and galacto-oligosaccharides (GOS), in fruit juices has shown promise in enhancing the growth and activity of LAB, thereby creating functional beverages that support digestive health. Despite numerous studies on fruit juice fermentation, there is limited data on the optimal pairing of probiotics and prebiotics to develop stable, nondairy functional drinks. This review underscores the potential of lactic acid fermentation and the integration of prebiotics and probiotics in fruit juices, highlighting the necessity for further research to optimize these combinations for enhanced health benefits and improved beverage stability.
  17. Ullah F, Irfan M, Khatoon S, Khalil S, Sher A, Alsulami N, et al.
    Braz J Biol, 2025;84:e287016.
    PMID: 39968987 DOI: 10.1590/1519-6984.287016
    The exposure of plant seeds to gamma radiation is a promising prospect to crop improvement through the manipulation of their genetic makeup. Previous studies have shed light on the potential of radiation to enhance the genetic variability. In this study, we investigated the effect of gamma radiation on Pisum sativum seeds under heavy metal (nickel chloride) stress to determine the changes in morpho-biochemical attributes. Morphological parameters such as germination and photosynthetic pigments while biochemical attributes such as protein content, sugar, phenolics, and flavonoids were determined. The results showed that gamma radiation, along with (NiCl2) has a pronounced effect on plant morphology and production. In the biochemical analysis of the range from 50 Gy to 100 Gy, photosynthetic pigments and proteins were significantly associated. Although the 50 Gy dose induced a partial reduction in sugar content while the 100 Gy dose demonstrated a slight improvement relative to the 50 Gy dose. However, the phenol content increased in response to 50 Gy, whereas the flavonoid content decreased compared to the control. In combination with heavy metal (50mM) at Gy doses, the protein, sugar, phenol, and flavonoid contents showed a gradual decrease with the increase in Gy doses. In conclusion, the current study based on observations suggests that the range of gamma radiation from 50 Gy to 100 Gy is suitable for causing the mutant form of seeds. However, further studies should be conducted to determine the precise mechanism, in order to be benefitted from full potential role of gamma radiation in improving productivity under heavy metal stress.
    MeSH terms: Flavonoids/analysis; Gamma Rays*; Phenols; Photosynthesis/drug effects; Photosynthesis/radiation effects; Plant Proteins; Stress, Physiological; Germination/drug effects; Germination/radiation effects; Metals, Heavy
  18. Boo HS, Sivarajan S, Nor Zahidah Mohd Tahir N, Bahar AD
    J Orofac Orthop, 2025 Feb 19.
    PMID: 39969598 DOI: 10.1007/s00056-024-00570-x
    BACKGROUND: With the constant improvement of photopolymerizable resin, direct three-dimensional (3D) printing of retainers and aligners (R&A) has gained popularity in recent years. The primary objective of this research was to systematically evaluate the evidence regarding the accuracy of 3D-printed R&A in terms of trueness and precision (T&P).

    METHODS: Electronic database searches were performed on PubMed, Science Direct, Web of Science, Scopus, Ovid, and Clinicaltrial.gov through 17 November 2023. The Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool was used to assess the risk of bias and applicability concerns of the methodologic quality of the articles.

    RESULTS: A total of 12 in vitro studies were selected. Five studies reported the accuracy of 3D-printed retainers and another seven reported on aligners. For the 3D-printed retainer group, only Naeem et al. [1] reported both T&P and found that stereolithography (SLA) and PolyJet photopolymer (PPP) printers demonstrated higher accuracy than digital light processing (DLP) and continuous digital light processing (cDLP) printers. For the 3D-printed aligners group, only Koenig et al. [2] reported both T&P and found that 3D-printed aligners fabricated with photopolymerizable polyurethane resin demonstrated higher trueness (0.14 ± 0.020 mm) compared to conventional thermoformed aligners (0.188 ± 0.074 and 0.209 ± 0.094).

    CONCLUSION: The existing digital workflow involving various printing materials, angulations, printing thickness, and printing technologies is feasible for the fabrication of direct 3D printing of R&A with variable degrees of accuracy. Most techniques worked within the clinically acceptable level of accuracy of 0.25 mm.

    TRIAL REGISTRATION NUMBER: PROSPERO CRD 42022312907.

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