Browse publications by year: 2025

  1. Damodaran T, Yahaya NS, Mordi MN
    Toxicol In Vitro, 2025 Apr 04;107:106062.
    PMID: 40188856 DOI: 10.1016/j.tiv.2025.106062
    Tocotrienol-rich fraction (TRF), a natural form of vitamin E derived from palm oil, possesses antioxidant properties. However, its potential embryonic developmental toxicity remains unclear. This study investigated TRF's toxicity using in silico methods and zebrafish embryos. Zebrafish embryos were exposed to TRF (31.25 to 2000 μg/mL) for 96 h post-fertilization (hpf). Mortality, hatching rate, heart rate, and morphological malformations were assessed at 24, 48, 72, and 96 hpf. In silico analysis predicted good pharmacokinetic properties and minimal side effects for five TRF constituents, except for hERG II inhibition, which is associated with cardiac toxicity. TRF exposure up to 96 hpf showed no embryotoxicity in zebrafish at ≤1000 μg/mL. However, TRF at concentrations of ≥1000 μg/mL significantly inhibited hatching rate at 72 hpf, indicating a delay in the hatching process. Additionally, 1000 μg/mL of TRF resulted in reduced heart rate and hypopigmentation in the embryos. Moreover, higher TRF concentrations (≥500 μg/mL) caused morphological malformations including spinal curvature, pericardial edema, and yolk sac edema, in the embryos. These findings suggest that TRF from palm oil is likely safe at concentrations below 500 μg/mL during embryonic development. However, the potential effects of long-term exposure and chronic toxicity warrant further investigation to ensure safety during early pregnancy.
  2. Yi Lim L, Shei-Dei Yang S
    J Pediatr Urol, 2025 Mar 21.
    PMID: 40189459 DOI: 10.1016/j.jpurol.2025.03.008
    INTRODUCTION: Uroflowmetry is useful in the evaluation of patients with suspected lower urinary tract dysfunction (LUTD). The pattern may serve as a guide to a specific condition, so it is critical to interpret it accurately and consistently. There are various definitions for staccato patterns, but they are primarily descriptive and result in low inter-observer reliability. Some authors stated that a staccato pattern must include a specific amount of "deep drops," "notches," "peaks," and "fluctuations." However, our observations show that these features are common even in uroflowmetry curves of healthy individuals.

    OBJECTIVE: To propose comprehensive criteria for defining "deep drop (DD)", assess the incidence of DD in uroflowmetry curves of healthy children and adolescents, and to evaluate the inter-rater agreement for this new definition.

    MATERIALS AND METHODS: This study defines DD as a "sudden decrease of flow ≥√Qmax followed by a rebound of flow ≥2 ml/s". 1787 participants, aged 5-18 years, took 3158 uroflowmetry measurements. Uroflowmetry with artefacts (n = 466) as illustrated in the Supplementary Figure and low voided volume (VV) (n = 1425) were excluded.

    RESULTS: Of the 1267 uroflowmetry curves included in the analysis, 16.0 %, 7.4 % and 7.3 % had one, two, and ≥three DDs, respectively. The incidence of any DD increased from 26.1 % at VV 50-75 ml of estimated bladder capacity (EBC) to 51.2 % at VV >150 % of EBC (see Supplementary Table). Excluding uroflowmetry with large voided volume (LVV), the incidence of deep drops decreased to 15.7 %, 6.7 %, and 5.2 % for one, two, and three deep drops, respectively. Among 261 participants with two uroflowmetry measurements, 53.3 % had no DD on both occasions, while 4.6 % and 1.5 % had ≥ two and ≥three DDs on both occasions, respectively.

    CONCLUSION: DDs are common in the uroflowmetry of healthy subjects, but ≥2 DDs are uncommon. Based on these findings, the occurrence of ≥2 DDs on uroflowmetry, particularly if observed repeatedly, may warrant further evaluation for possible LUTD. Further research is needed to determine the validity of the DD definition and number of DD required to define the staccato pattern by correlating the urodynamic findings of patients with DD.

  3. Adil NFBN, Murthy JK, Mohamed IBN, Jamil TRB, Mohamed RMBP
    Tob Induc Dis, 2025;23.
    PMID: 40190367 DOI: 10.18332/tid/200071
    INTRODUCTION: Smoking has become a leading preventable cause of premature death and morbidity worldwide, with 8 million people dying each year because of tobacco. In Malaysia, a 24-week standard smoking cessation program is available to help smokers. Teleconsultation was introduced into this program during the COVID-19 pandemic by using internet-based video counseling to reduce the number of clinic visits. This study aimed to evaluate the outcomes of hybrid virtual consultation for smoking cessation programs among patients with nicotine dependence.

    METHODS: A retrospective cross-sectional study was conducted where all the active smokers registered in the smoking cessation program from 2018 to 2023 were recruited. They were grouped into face-to-face interventions and hybrid virtual consultations. All data were obtained from the smoking cessation program registry. The primary outcome was point abstinence (PA) at week 7 (1-month post-quit date), biochemically verified with carbon monoxide (CO) Smokerlyzer for both face-to-face and hybrid groups.

    RESULTS: A total of 156 participants were included in this study, including face-to-face (99 participants) and hybrid virtual consultation (57 participants). The mean age of face-to-face and hybrid group participants was 51 and 48 years, respectively. In general, hybrid virtual consultation was more feasible, as evidenced by a lower defaulter rate and a higher rate of participants graduating at the end of the program than face-to-face consultation. The effectiveness of smoking cessation was also higher in hybrid consultation, with a higher abstinence rate at weeks 4 and 7, with percentages of 42.1% and 56.1%, respectively. Additionally, the hybrid group maintained a high continuous abstinence rate (CAR) from week 7 to 24, with a percentage of 56.1%.

    CONCLUSIONS: Hybrid virtual consultation was more effective, as evidenced by higher smoking cessation at week 7 (1-month post-quit date) and CAR from week 7 to week 24 compared to the face-to-face group. Telemedicine or teleconsultation should be easily available for smoking cessation programs, and healthcare providers should consider incorporating hybrid models into them to fully utilize the program and improve outcomes.

  4. Erysha Sabrina Jefferi N, Afifah Shamhari A, Abd Hamid Z, Balkis Budin S, Shima Taib I
    Int J Reprod Biomed, 2025 Jan;23(1):17-32.
    PMID: 40190456 DOI: 10.18502/ijrm.v23i1.18187
    Bisphenols (BP) are endocrine-disrupting chemicals that cause adverse health effects, including testicular steroidogenesis disturbance. Cyclo-oxygenase-2 and nuclear factor erythroid 2-related factor 2 are the target molecules involved in testicular steroidogenesis disturbance via inflammation and oxidative stress (OS), respectively. Interestingly, endoplasmic reticulum (ER) stress was found to be involved in various pathological conditions. However, the mechanisms involved in BP-induced testicular steroidogenesis disturbance remain unclear. Therefore, this research investigates the key mechanisms underlying BP-induced testicular steroidogenesis disturbances. We focus on 3 critical pathways: inflammation, OS, and ER stress. Our findings demonstrate that BP exposure triggers inflammatory responses by targeting the cyclo-oxygenase-2 molecules that impair Leydig cell function. Concurrently, we observed that BP-increased OS via inhibition of nuclear factor erythroid 2-related factor 2, further disrupting steroidogenic enzyme activity. Additionally, ER stress is activated in response to BP exposure, leading to impaired protein synthesis and exacerbating steroidogenic dysfunction. This review elucidates the interlinkage between inflammation, OS, and ER stress in BP-induced testicular steroidogenesis disturbance in which reactive oxygen species is proposed to be the main culprit in linking these 3 mechanisms. These insights provide a crucial foundation for understanding the reproductive toxicology of BPs and inform future strategies for mitigating their effects on male reproductive health.
  5. Mehdi M, Raza SH, Yousaf M, Li B, Zaman U, Riaz S
    Risk Manag Healthc Policy, 2025;18:1133-1145.
    PMID: 40190730 DOI: 10.2147/RMHP.S501423
    BACKGROUND AND PURPOSE: Concern over the low hepatitis immunization rates is growing. It is generally believed that misinformation and disinformation are the main barriers to the success of mass immunization campaigns. This study intends to investigate the matter of online misinformation and disinformation about hepatitis vaccination and its implications.

    MATERIAL AND METHODS: The researchers employed a cross-sectional research design vis-à-vis a web-based survey method to collect data. Data were collected from a nationally representative sample of 1931 internet users across Pakistan. Participation in the study was voluntary. Data collection continued for four months, from Mar 1, 2024, to Jun 30, 2024.

    RESULTS: The "results" of structural equation modeling show that misinformation and disinformation substantially structure risk perception regarding hepatitis, which, in turn, impacts problem recognition, involvement recognition, and constraint recognition. Besides, the outcomes uncovered that the chain of problem identification impacts situational motivation, which drives risk communication behaviors. Also, these risk communication behaviors are closely linked to individuals' motivation to receive the hepatitis vaccine.

    CONCLUSION: This research concludes that managing misinformation and disinformation via strategically designed, valid, and reliable digital interventions improves the public's active response regarding willingness to get vaccinated against hepatitis. This study contributes to increasing public acceptance of the hepatitis vaccine by utilizing reliable and valid digital interventions to achieve a more active public health response.

  6. Doshi D, Kumar S, Patel B, Chaudhari D, Patel S, Hirani T, et al.
    Cureus, 2025 Apr;17(4):e81694.
    PMID: 40190829 DOI: 10.7759/cureus.81694
    This is a comprehensive narrative assessment of micronutrient relevance for periodontal health. The periodontium is a crucial and intricate structure that supports the tooth within the alveolar bone. Composed of four tissues with distinct embryological origins- the alveolar bone, cementum, gingiva, and periodontal ligament - the periodontium plays a fundamental role in maintaining dento-alveolar function and homeostasis. Periodontitis is gaining attention due to its widespread occurrence worldwide. This inflammatory condition disrupts the balance between the host immune response and microbial virulence factors. Connective tissue production and host defenses require proteins for periodontal health. Vegetable proteins support periodontal health by promoting tissue repair and immune function, while excessive dietary fats can exacerbate inflammation and increase the risk of periodontal disease (PD). Inflammation is further compounded due to dietary animal fats, which contribute to the risk of PD. Omega-3 fatty acids are well-known for their anti-inflammatory effects, which help reduce periodontal inflammation. Adequate intake of essential minerals and vitamins is required for maintaining periodontal health. Vitamins K, D, and A are crucial in maintaining oral epithelial integrity, facilitating bone development, and supporting overall tissue maintenance. Inadequate calcium (Ca²⁺) and magnesium (Mg²⁺) levels have been associated with severe PD. Antioxidants such as vitamin C help alleviate inflammation in periodontal tissues. As recognized by dental professionals, nutritional status plays a considerable role in an individual's risk of developing PD.
  7. Tan PH, Ja'apar AN, Munusamy MM, Sallehuddin A
    Cureus, 2025 Mar;17(3):e80216.
    PMID: 40190904 DOI: 10.7759/cureus.80216
    Several surgical approaches have been proposed to correct the transposition of the great arteries (TGA) with ventricular septal defect (VSD) and left ventricular outflow tract obstruction (LVOTO). Unlike the traditional procedures such as the Rastelli and Réparation à l'Etage Ventriculaire (REV) procedure, which often necessitate reoperation due to right or left ventricular outflow tract obstruction, the Bex-Nikaidoh procedure involves the translocation of both the aortic and pulmonary roots to achieve anatomically correct outflow alignment for both ventricles. Although technically demanding, this technique effectively addresses the anatomical challenges such as inlet or restrictive VSD and hypoplastic right ventricle. Recent studies indicate encouraging mid-term results, including high survival rate, preserved left ventricular function, and lower incidence of reoperation. We present a case report of a child with TGA, VSD, and pulmonary stenosis who successfully underwent the Bex-Nikaidoh procedure, detailing our operative technique, perioperative assessment, and management, thereby contributing to the evolving landscape of surgical repair for complex TGA with VSD and LVOTO.
  8. Sallam M, Al-Khatib AO, Al-Mahzoum KS, Abdelaziz DH, Sallam M
    Clin Pharmacol, 2025;17:29-47.
    PMID: 40191019 DOI: 10.2147/CPAA.S513282
    INTRODUCTION: Malaria remains a persistent challenge in global health, disproportionately affecting populations in endemic regions (eg, sub-Saharan Africa). Despite decades of international collaborative efforts, malaria continues to claim hundreds of thousands of lives each year, with young children and pregnant women enduring the heaviest burden. This concise review aimed to provide an up-to-date assessment of malaria vaccines progress, challenges, and future directions.

    METHODS: A PubMed/MEDLINE search (2015-2024) was conducted to identify studies on malaria vaccine development, implementation barriers, efficacy, and vaccination hesitancy. Clinical trials, reviews, and global health reports were included based on relevance to the review aims. No strict inclusion criteria were applied, and selection was guided by key review themes and policy relevance.

    RESULTS: The introduction of pre-erythrocytic malaria vaccines (RTS,S/AS01 and R21/Matrix-M), represents an important milestone in malaria control efforts with promising results from the erythrocytic vaccine RH5.1/Matrix-M in recent clinical trials. However, the approval of these vaccines is accompanied by significant challenges such as the limited efficacy, the complexity of multi-dose regimens, and numerous barriers to widespread implementation in resource-limited settings. The review identified the complex challenges to broad malaria vaccination coverage, including logistical barriers, healthcare infrastructure effect, financial limitations, malaria vaccine hesitancy, among other obstacles in malaria-endemic regions. Promising developments in malaria vaccination, such as next-generation candidates (eg, mRNA-based vaccines), hold the potential to offer improved efficacy, longer-lasting protection, and greater scalability. There is a critical need to integrate malaria vaccination efforts with established malaria control interventions (eg, insecticide-treated bed nets, vector control strategies, and anti-malarial drugs).

    CONCLUSION: Achieving sustained control of malaria morbidity and mortality will require strong global collaboration, sufficient funding, and continuous efforts to address inequities in access and delivery of malaria control measures including the malaria vaccines.

  9. Yogabalan K, Appalanaido GK, Seng CE, Jalil JB, Jayamani J, Ishak NH, et al.
    J Contemp Brachytherapy, 2025 Feb;17(1):28-32.
    PMID: 40191051 DOI: 10.5114/jcb.2025.148297
    PURPOSE: The aim of this study was to retrospectively analyze and report on dose-volume and clinical toxicity of liver high-dose-rate interstitial brachytherapy (HDR-IBT) used in diaphragm and lung tissue.

    MATERIAL AND METHODS: Computed tomography (CT)-based liver HDR-IBT using Oncentra Brachy treatment planning system (TPS) plans of patients with malignant liver tumor (MLT) from September 2018 to June 2023 were reviewed to identify patients, whose diaphragm and lung tissue were within 100% prescription isodose. These organs at risk (OARs) were contoured in axial CT slices. Maximum point dose (Dmax), dose to 0.2 cc, 0.5 cc, 1 cc (D0.2cc, D0.5cc, D1cc), and volume receiving 30 Gy and 50 Gy (V30Gy and V50Gy) were analyzed. Toxicity data of these patients were retrieved from hospital electronic records.

    RESULTS: The analysis included 27 patients with 43 and 36 MLTs, whose 100% prescription isodose of liver HDR-IBT plan was within diaphragm and lung tissue. Median prescription dose was 25 Gy (range, 15-25 Gy) in single-fraction. Median Dmax, D0.2cc, D0.5cc, and D1cc of the diaphragm were 302 Gy (range, 54-396 Gy), 68 Gy (range, 38-234 Gy), 48 Gy (range, 32-128 Gy), and 35 Gy (range, 27-88 Gy), while for the lung, 90 Gy (range, 39-295 Gy), 55 Gy (range, 32-207 Gy), 44 Gy (range, 29-117 Gy), and 34 Gy (range, 25-79 Gy), respectively. Median V30Gy and V50Gy for the diaphragm were 1.1 cc (range, 0-5.8 cc) and 0.2 cc (range, 0-2.5 cc), while for the lung, 0.8 cc (range, 0-10.1 cc) and 0.1 cc (range, 0-2.3 cc), receptively. Two patients with repeated HDR-IBT sessions received cumulative Dmax diaphragm of 698 Gy and 792 Gy. At median follow-up of 23 months, no patient reported any suspicious symptom of radiation-induced diaphragm or lung injury.

    CONCLUSIONS: This is the first publication reporting diaphragm and lung tissue dose-volume and clinical toxicity in liver HDR-IBT. Small volume of diaphragm and lung tissue tolerated extreme high radiation doses [5 times of stereotactic body radiotherapy (SBRT) range in single fraction] without clinically significant toxicity. A standardized reporting for diaphragm and lung dose volume is needed for future liver HDR-IBT studies. The results of the current study can be employed in future for expanded indication of brachytherapy, such as CT-guided trans-thoracic lung brachytherapy.

  10. Liao B, Yang Q, Li D, Ooi CT
    Clin Med Insights Case Rep, 2025;18:11795476251332440.
    PMID: 40191106 DOI: 10.1177/11795476251332440
    A case of spontaneous bilateral internal carotid artery dissection (ICAD) in a 36-year-old male, potentially linked to COVID-19, is reported. Initially presenting with excessive daytime sleepiness, an atypical symptom for ICAD, the patient lacked focal neurologic deficits. Diagnostic evaluation revealed severe stenosis and dissecting aneurysms in both carotid arteries. Treatment included anticoagulation, corticosteroid therapy, and subsequent endovascular stent placement. This case underscores the importance of considering ICAD as a potential complication of COVID-19, especially in patients with atypical symptoms. Further research is needed to understand the underlying mechanisms and optimize treatment strategies.
  11. Bagchi C, Patwardhan SD, Iglauer S, Ben Mahmud H, Ali MFJ
    ACS Omega, 2025 Apr 01;10(12):11658-11696.
    PMID: 40191361 DOI: 10.1021/acsomega.4c10442
    The need and demand for energy are ever increasing with the rapid urbanization of the global population. Near-future economic development and consequent expected industrialization are the strongest indicators of the rising energy requirement. Though fossil fuel and coal are catering toward a major chunk of energy demand, their negative or adverse impact in terms of emission of greenhouse gases (GHGs) and carbon dioxide (CO2) is causing global warming and imbalance in environmental conditions, leading to a search for commercially viable alternate energy types. As renewable energies like solar and wind are weather-dependent, the search for an alternate energy supply needs to be done even more urgently. Hydrogen (H2), being a carrier of alternate energy and not an energy source, can deliver or store a significant amount of energy. The stored energy can be utilized for the generation of power, electricity, and heat. Further, hydrogen is very pure in nature and therefore is widely useful from a flexibility and efficiency standpoint. It represents the predominant constituent within the natural environment, totaling about three-fourths of the universe. Storage of hydrogen has gained significance in recent times, and subsurface storage is being looked upon as a viable alternative. However, the successful storage of hydrogen in the subsurface is a function of critical parameters like wettability, capillary pressure, relative permeability, diffusion, microbial activities, etc., which themselves are dependent upon rock types with their corresponding mineralogical compositions, along with associated pressures and temperatures. This work critically reviews the impact that these parameters would have on the storability of hydrogen in the subsurface, evaluates the best possible solution, and recommends a future course of action through insights derived, which need to be considered while considering underground hydrogen storage.
  12. Alsaady M, Waqas S, Zeeshan MH, Almarshoud MA, Maqsood K, Abdulrahman A, et al.
    ACS Omega, 2025 Apr 01;10(12):11972-11979.
    PMID: 40191367 DOI: 10.1021/acsomega.4c09251
    This study investigates the fabrication and optimization of mixed matrix membranes (MMMs) composed of NH2-MIL-125(Ti), a metal-organic framework (MOF), dispersed within a polysulfone (PSf) polymer matrix, for efficient CO2/CH4 separation. The MMMs were prepared by using a solution casting method, and their morphology and gas separation performance were systematically characterized. The effect of MOF addition into the polymer matrix, gas permeability, and selectivity were evaluated using a gas permeation setup. Results indicate that incorporating NH2-MIL-125(Ti) nanoparticles enhances the selectivity of the membranes for CO2 over CH4 compared to pure polymer membranes while maintaining acceptable permeability. The membrane morphology demonstrates the uniform distribution of the filler in the polymer matrix. The PSf/NH2-MIL-125(Ti)-15% membrane showed exceptional CO2 permeability and selectivity performance. Specifically, it achieved a CO2 permeability of 19.17 Barrer. Additionally, it exhibited a CO2/CH4 selectivity of 31.95, indicating its ability to effectively differentiate between the CO2 and CH4 gases, which is critical for applications such as natural gas purification and carbon capture. Furthermore, the MMMs produced in this study showed outstanding resistance to CO2 plasticization. The PSf/NH2-MIL-125(Ti)-15% membrane demonstrated superior pressure resistance, withstanding up to 14 bar without significant performance degradation compared to the pristine PSf membrane, which succumbed to plasticization at 4 bar. The enhanced plasticization resistance is attributed to incorporation of NH2-MIL-125(Ti) into the PSf matrix. The combination of high CO2 permeability, excellent selectivity, and robust plasticization resistance positions the PSf/NH2-MIL-125(Ti)-15% membrane as a highly effective solution for CO2 separation applications. The results underscore the potential of these MMMs to achieve significantly better performance metrics than traditional PSf membranes, making them a promising option for industrial gas separation processes.
  13. Yong JY, Zaidan NZ, Gan WF
    AIDS Res Treat, 2025;2025:7548833.
    PMID: 40191382 DOI: 10.1155/arat/7548833
    Introduction: We aimed to evaluate the clinical presentation and diagnosis process of all newly diagnosed human immunodeficiency virus (HIV) patients and conduct a 'look back' for barriers to care to aid a greater understanding of interventions to reduce late presentation. Methods: We evaluated 102 patients with newly diagnosed HIV who were referred to Melaka Hospital's infectious disease (ID) team from January 2021 to December 2022 via retrospective case record review. They were categorised into late presenters (LPs) and nonlate presenters (NLPs). LP is defined as persons presenting for care with a cluster of differentiation 4 (CD4) count below 350 cells/μL or presenting with an acquired immunodeficiency syndrome (AIDS) defining event, regardless of the CD4 cell count. Demographic characteristics, individual and healthcare system barriers and treatment outcomes were evaluated. Results: There were 89.2% of LPs, with 56.9% presenting with opportunistic infection (OI). Median CD4 for LPs upon diagnosis was 53 cells/μL. Pneumocystis jirovecii pneumonia was the most common presenting OI. Most were men who had sex with men (MSM) with more university graduates among the NLPs compared to LPs (36.4% vs. 8.8%, p 0.02). 9.9% of LPs experienced pitfalls during healthcare consultation, leading to late presentation, which was labelled as adverse events. LP's median time from diagnosis to first ID consultation was 7 days, and all patients' median duration of antiretroviral therapy (ART) initiation was 24 days. 82.4% of the patients were still on follow-up, with 69.6% achieving virological suppression at 6 months of ART. The mortality rate was 5.9%, all of which were LPs, and most were MSM. Conclusions: Late presentation remains challenging, with 9.9% of potent preventable adverse events. Morbidity meetings are crucial for immediate feedback to involved healthcare providers. Community-based organisations also play an essential role in identifying and providing linkage of high-risk groups to early HIV screening and diagnosis.
  14. Veerabhadrappa SK, Vadivel JK, Roodmal SY, Ismail UKB, Jain VK
    Imaging Sci Dent, 2025 Mar;55(1):65-71.
    PMID: 40191394 DOI: 10.5624/isd.20240196
    PURPOSE: The bisecting angle technique (BAT) encounters difficulties in positioning the X-ray cone and aligning the central beam with the tooth. To address this, a rectangular laser featuring a pointed light was integrated into the intraoral X-ray cone. This study evaluated its effectiveness in improving radiograph quality and minimizing errors.

    MATERIALS AND METHODS: Fifty fifth-year Bachelor of Dental Surgery students were divided into 3 groups. Group 1 (n=16) used the paralleling technique, group 2 (n=17) employed the conventional BAT, and group 3 (n=17) utilized the laser-assisted BAT on mannequins. Two independent oral radiologists assessed the quality of the radiographs, categorizing the images as either diagnostically acceptable or not acceptable. Inter-group comparisons of quality and error rates were conducted using the chi-square test (significance level: P<0.05).

    RESULTS: The paralleling technique group produced 77.5% diagnostically acceptable radiographs and 22.5% that were not diagnostically acceptable. These percentages were 65.3% and 34.7%, respectively, in the conventional BAT group and 75.3% and 24.7%, respectively, in the laser-assisted BAT group, showing results similar to the paralleling technique group. The quality of radiographs differed significantly among the groups (P<0.05). The percentage of error-free radiographs was 38.1% in the paralleling technique group, 20.6% in the conventional BAT group, and 40.0% in the laser-assisted BAT group, with these differences being statistically significant (P<0.05).

    CONCLUSION: The device produced higher acceptability and fewer radiographic errors than the conventional BAT technique, suggesting accurate adjustment of the X-ray cone and central beam to the desired teeth.

  15. Kurniawan A, Saelung M, Rizky BN, Chusida A, Prakoeswa BFWR, Nefertari G, et al.
    Imaging Sci Dent, 2025 Mar;55(1):28-36.
    PMID: 40191398 DOI: 10.5624/isd.20240134
    PURPOSE: This study employed a convolutional neural network (CNN) algorithm to develop an automated dental age estimation method based on the London Atlas of Tooth Development and Eruption. The primary objectives were to create and validate CNN models trained on panoramic radiographs to achieve accurate dental age predictions using a standardized approach.

    MATERIAL AND METHODS: A dataset of 801 panoramic radiographs from outpatients aged 5 to 15 years was used. A CNN model for dental age estimation was developed using a 16-layer CNN architecture implemented in Python with TensorFlow and Scikit-learn, guided by the London Atlas of Tooth Development. The model included 6 convolutional layers for feature extraction, each followed by a pooling layer to reduce the spatial dimensions of the feature maps. A confusion matrix was used to evaluate key performance metrics, including accuracy, precision, recall, and F1 score.

    RESULTS: The proposed model achieved an overall accuracy, precision, recall, and F1 score of 74% on the validation set. The highest F1 scores were observed in the 10-year and 12-year age groups, indicating superior performance in these categories. In contrast, the 6-year age group demonstrated the highest misclassification rate, highlighting potential challenges in accurately estimating age in younger individuals.

    CONCLUSION: Integrating a CNN algorithm for dental age estimation represents a significant advancement in forensic odontology. The application of AI improves both the precision and efficiency of age estimation processes, providing results that are more reliable and objective than those obtained via traditional methods.

  16. Prashar N, Mohammed SB, Raja NS, Mohideen HS
    3 Biotech, 2025 May;15(5):116.
    PMID: 40191455 DOI: 10.1007/s13205-025-04270-0
    The COVID-19 pandemic highlighted the potential of peptide-based therapies as an alternative to traditional pharmaceutical treatments for SARS-CoV-2 and its variants. Our review explores the role of therapeutic peptides in modulating immune responses, inhibiting viral entry, and disrupting replication. Despite challenges such as stability, bioavailability, and the rapid mutation of the virus, ongoing research and clinical trials show that peptide-based treatments are increasingly becoming integral to future viral outbreak responses. Advancements in computational modelling methods in combination with artificial intelligence will enable mass screening of therapeutic peptides and thereby, comprehending a peptide repurposing strategy similar to the small molecule repurposing. These findings suggest that peptide-based therapies play a critical and promising role in future pandemic preparedness and outbreak management.
  17. Roney M, Uddin MN, Sapari S, Razak FIA, Huq AKMM, Zamri NB, et al.
    3 Biotech, 2025 May;15(5):107.
    PMID: 40191458 DOI: 10.1007/s13205-025-04287-5
    Diabetes mellitus (DM) is a serious worldwide health issue in the twenty-first century. Additionally, DM, a metabolic endocrine illness that affects the digestion of proteins, carbohydrates, and lipids, has a death rate of 4.9 million individuals globally. This study aims to find anti-diabetic inhibitor for type 2 diabetes (T2D) that inhibits the dipeptidyl peptidase IV (DPP-IV) enzyme using in silico methods. From a range of published literature sources, thirty (30) isoxazole derivatives of UA (IDUA) were selected for this study. To ascertain the possible inhibitory effects of IDUA, ADMET, molecular docking, density functional theory analyses, molecular dynamic simulation and MM/PBSA were conducted. Eleven compounds (1, 2, 3, 4, 7, 13, 18, 21, 22, 24, and 27) were selected from the ADMET study, which were subjected to perform molecular docking against the DPP-IV enzyme of T2D, and findings indicated two compounds (compound 2 and compound 3) showed comparable binding affinity with the reference compound "Linagliptin". In contrast to the reference molecule, which had a binding affinity of - 8.6 kcal/mol against DPP-IV, compound 2 and compound 3 have binding affinities of - 8.1 and - 8.0 kcal/mol, respectively. Furthermore, based on Lipinski's Rule of Five, ELUMO, EHOMO, band energy gap, drug-likeness and DFT-based studies demonstrated druggability and high reactivity for these compounds. In addition, the molecular dynamic (MD) techniques to confirm that docked complexes remained stable and that the binding orientation obtained during docking tests were accurate. These compounds may be investigated in vitro and in vivo for the development of potential DPP-IV of T2D inhibitors.

    SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-025-04287-5.

  18. Wali MH, Naif HM, Abdul Rahim NA, Yunus MA
    Adv Virol, 2025;2025:8864776.
    PMID: 40191805 DOI: 10.1155/av/8864776
    Molecular evaluation of the respiratory syncytial virus (RSV) genome is one of the common strategies applied to understand the viral pathogenicity and control its spreading. In this study, we carried out molecular evaluation on the targeted fusion (F) gene region in the RSV-positive samples of Iraqi patients during the autumn and winter of 2022/2023. One hundred and fifty patients with lower respiratory tract infections were screened for RSV using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Sanger sequencing was performed on the RSV-positive samples targeting 1061 nucleotides (from nucleotide 6168 to 7228 within the RSV genome) and 1000 nucleotides (from nucleotide 6122 to 7121 within the RSV genome) of the F gene region for RSV-A and RSV-B, respectively. The results showed some nucleotide changes within the targeted F gene, which were grouped in distinct clade, closely related to isolates from Austria, Argentine, Finland, and France through phylogenetic analysis. In silico protein modeling using the SWISS-MODEL and I-TASSER web tools based on nonsynonymous changes of amino acid sequence showed some good-predicted models that can be utilized for antiviral screening. In summary, the identified nucleotide variations in the F gene could influence vaccine development as the F protein is the primary target for the major antigen of RSV. Molecular surveillance data of RSV local isolates are also essential for studying new genomic changes and enable the prediction of potential new antiviral agents.
  19. Abdul Rahman N, Rajaratnam V, Peters RMH, Abdullah MR, Morgan K
    Glob Public Health, 2025 Dec;20(1):2488889.
    PMID: 40191937 DOI: 10.1080/17441692.2025.2488889
    Leprosy, though curable, continues to present physical and psychosocial challenges for those affected. This study explored how individuals affected by leprosy in Malaysia navigated daily life, societal expectations, and personal challenges through the walk-and-talk technique, a participant-led qualitative approach that fostered spontaneous storytelling and reflexivity. By guiding researchers through their environments, participants shaped the narrative, shifting power dynamics and enabling authentic dialogue. Six participants from Kelantan and Selangor shared their everyday activities, aspirations, and coping strategies. Video documentation was used as a supplementary tool to capture non-verbal cues and contextual interactions. Visual, auditory, and field data were transcribed and thematically analysed using NVivo 12, revealing key themes, including resilience and perseverance, employment and economic sustenance, stigma, family support, and community engagement. Work emerged as central to their well-being, fostering dignity and providing financial stability. However, self-imposed stigma limited social inclusion, while strong familial and community bonds provided crucial support. These findings highlighted the effectiveness of the walk-and-talk technique in capturing rich qualitative data and emphasised the need for tailored interventions to address stigma, enhance social inclusion, and support the well-being and economic stability of individuals affected by leprosy.
    MeSH terms: Adaptation, Psychological; Adult; Female; Humans; Malaysia; Male; Middle Aged; Social Support; Qualitative Research; Social Stigma
  20. Pirasteh N, Farpour HR, Najafi P, Jabbedari MH
    J Ultrasound, 2025 Mar 09.
    PMID: 40059249 DOI: 10.1007/s40477-025-01004-z
    BACKGROUND: Healthy tendons are crucial for sportspersons to prevent injuries. While resistances exercise is known to increase muscle size and composition, its impact on tendon thickness remains unclear. This study investigated potential differences in tendon thickness between bodybuilders and non-sportsperson control group and their association with training intensity/duration in bodybuilders.

    METHODS: Forty men in the age range 18 to 40 were included in the study. Fifteen bodybuilders (2-3 weekly upper body resistance training sessions, moderate-to-high intensity, > 6 months) and 25 non-sportsperson controls participated. All were free of tendon injuries in the studied muscles (biceps, supraspinatus, infraspinatus). Two independent physiatrists measured tendon thickness using ultrasound.

    RESULTS: No significant differences in tendon thickness were found between bodybuilders and non-sportsperson controls (dominant/non-dominant sides). However, within the bodybuilding group, tendon thickness showed strong positive correlations with both training intensity (r = 0.59-0.84) and exercise duration (r = 0.71-0.88).

    CONCLUSION: This study found no overall group differences in tendon thickness between bodybuilders and non-sportsperson controls. However, for bodybuilders, increased training intensity and duration were associated with thicker tendons. Further research with larger samples and advanced techniques is warranted to understand the complex relationship between resistance training and tendon adaptations.

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