Browse publications by year: 2025

  1. Ahmad MM, Ali Shah ZB, Nies HW
    Comput Biol Med, 2025 Apr 04;190:110122.
    PMID: 40187181 DOI: 10.1016/j.compbiomed.2025.110122
    Drugs are essential chemical substances used to treat, prevent, or diagnose diseases. However, the co-administration or simultaneous use of multiple drugs can lead to complex interactions known as Drug-Drug Interactions (DDIs). Adverse DDIs are a major concern in the pharmaceutical industry, posing significant health risks by potentially causing toxicity or negatively impacting therapeutic efficacy. Identifying all DDIs through clinical trials alone is not feasible, making computational approaches crucial for predicting and understanding these interactions. Molecular structures and biomedical entities represented as knowledge graphs (KGs) have been widely used for DDI prediction. This review first examines diverse molecular representations commonly utilized in DDI prediction. It then highlights KG-based approaches, emphasizing their ability to integrate heterogeneous biomedical data and provide comprehensive structural and relational insights into drugs, proteins, and other biological entities. However, predictive models frequently encounter challenges when dealing with drugs with limited interaction data or unknown structures, resulting in a cold-start scenario that negatively impacts model generalization. Consequently, this review also discusses methods to address the cold-start scenario in DDI prediction. Finally, key findings and potential directions for enhancing DDI prediction are presented.
  2. Alias A, Amin KAM, Heng LS, Nor SMM, Omar WBW, Ibrahim YS, et al.
    Mar Pollut Bull, 2025 Apr 04;215:117931.
    PMID: 40187200 DOI: 10.1016/j.marpolbul.2025.117931
    Microplastics pose serious risks for aquatic organisms such as fishes, shrimps and bivalves. Bivalves are particularly vulnerable due to their filter-feeding strategy and sedentary life. While the microplastic bioaccumulation in bivalves has been well documented, the effects of microplastics accumulation on bivalve's gut microbiome in tropical sea waters remains poorly understood. To fill this knowledge gap, a 10-day feeding experiment with 13 mg L-1 polyethylene terephthalate particles was conducted using three commercially important bivalve species: Anadara cornea, Geloina expansa, and Meretrix meretrix taken from two contrasting locations (brackish water in protected Setiu Wetlands compared to open water in Kertih River) to investigate the effect of microplastic pollution on diversity and composition of gut prokaryotes using 16S rRNA amplicon sequencing. The results showed that alpha diversity of gut prokaryotes differed among species after microplastic exposure. For example, microplastic exposure increased operational taxonomic units (OTUs) richness of gut prokaryotes in G. expansa compared to A. cornea and M. meretrix during 10-day treatment. Community structure of prokaryotic community in bivalves gut showed strong divergence between Setiu Wetlands and Kertih River. Significant effects of microplastic exposure on relative abundance of prokaryotic phyla were also observed. Gut microbiome of G. expansa showed increase of relative abundance of Archaea and Firmicutes after microplastic exposure. The results suggest that microplastic treatment promotes dominance of certain bacterial species, likely those with plastic-metabolizing capabilities, potentially boosting bivalve resilience to microplastic contamination.
  3. Wu L, Li X, Ismail HN, Guo P, Yang J
    Reprod Health, 2025 Apr 05;22(1):46.
    PMID: 40188046 DOI: 10.1186/s12978-025-02001-5
    BACKGROUND: Breastfeeding provides health benefits for both mothers and children. However, most families face challenges in initiating and maintaining breastfeeding owing to psychosocial factors. Despite the importance of these factors and their practical implications, comprehensive review research based on a robust theory in this area is lacking. This narrative review addresses this research gap by exploring the utility of the extended theory of planned behavior (ETPB) in understanding and promoting breastfeeding.

    METHODS: A systematic search was conducted using the relevant Web of Science, Scopus, EBSCO, PubMed, and PsycINFO; 22 studies published between January 2000 and March 2023 were identified.

    RESULTS: These included studies understanding the psychosocial factors of breastfeeding by introducing the ETPB framework, incorporating psychosocial factors such as affective attitude, instrumental attitude, injunctive norms, descriptive norms, perceived behavioral control, breastfeeding self-efficacy, moral norms, breastfeeding knowledge, and breastfeeding self-identity. Based on these factors, intervention strategies for breastfeeding enhancement were categorized into professional breastfeeding education, interpersonal social support, and personalized breastfeeding services.

    CONCLUSIONS: The 22 breastfeeding studies related to the ETPB model demonstrate its effectiveness in explaining the psychosocial factors influencing breastfeeding intention and duration. The model's application in intervention studies suggests that more diverse and proactive approaches are required to empower mothers. Future research should address societal context and cultural influences and expand interventions beyond essential information provision.

    MeSH terms: Female; Humans; Health Knowledge, Attitudes, Practice*; Psychological Theory; Social Support
  4. Chen JH, Chen MF, Zain NM, Yap CY, Yusuf A, Ying BH
    BMC Public Health, 2025 Apr 05;25(1):1291.
    PMID: 40188096 DOI: 10.1186/s12889-025-22472-2
    BACKGROUND: Improving the health self-management ability (HSMA) of older adults is a feasible strategy for addressing population ageing and the high incidence of non-communicable diseases. Identifying psychosocial factors that facilitate better self-management of health is key to developing effective interventions. This study explored whether self-efficacy mediates the relationship between family function and HSMA and whether this mediation is moderated by personal income.

    METHODS: A multicentre cross-sectional survey was conducted with 596 participants (response rate of 97.2%) in the mountainous Lishui region of China by using a multi-stage, stratified, cluster-sampling procedure. The survey included questions on sociodemographic characteristics, the Adult Health Self-Management Skills (Ability) Rating scale, family APGAR scale and General Self-Efficacy (GSE) scale. Data were analysed using descriptive statistics, Spearman's correlation, and multiple regression analysis with mediation and moderated mediation models.

    RESULTS: The average score for HSMA, family function, and GSE were 149.6 ± 18.1 (out of 190), 8.2 ± 2.2 (out of 10), and 24.0 ± 6.0 (out of 40), respectively, which indicate moderate HSMA and family function levels and low GSE among older adults in Lishui. Significant correlations were observed between family function and GSE (rs = 0.150, P 

    MeSH terms: Aged; Aged, 80 and over; China; Cross-Sectional Studies; Female; Humans; Male; Middle Aged; Surveys and Questionnaires; Self Efficacy*
  5. Chang WH, Kim TW, Kim HS, Hanapiah FA, Lee JW, Han SH, et al.
    Sci Rep, 2025 Apr 05;15(1):11671.
    PMID: 40188310 DOI: 10.1038/s41598-025-96084-6
    Exoskeletons have been proposed for potential clinical use to improve ambulatory function in patients with stroke. The aim of an interim analysis of an international, multicenter, randomized, controlled trial was to investigate the short-term effect of overground gait training using a torque-assisted exoskeleton in subacute stroke patients with severe ambulatory functional impairment. Data from a total of 93 subacute stroke patients with severe ambulatory functional impairment were analyzed. All participants received a total of 20 sessions; five sessions per week for 4 weeks. The robot-assisted gait training (RAGT) group received 30 min of conventional gait training and 30 min of gait training using an exoskeleton (ANGEL LEGS M20, Angel robotics, Co., Ltd.), while the control group received 60 min of conventional gait training. Functional assessments were conducted before and immediately after the final intervention by a rater blinded to group assignment. Overground gait training with a torque-assisted exoskeleton in this study showed improvement in gait function comparable to conventional gait rehabilitation in subacute stroke patients, with additional gains in lower extremity strength. These findings suggest that the overground gait training with a torque-assisted exoskeleton might be a potential intervention for subacute stroke patients.Clinical Trial Registration: NCT05157347 (the first registration (10/12/2021)).
    MeSH terms: Exoskeleton Device*; Aged; Female; Humans; Male; Middle Aged; Treatment Outcome; Recovery of Function
  6. Siau CS, Lester D, Lew B, Kõlves K, Huen JMY, Gunn JF, et al.
    Asia Pac J Public Health, 2025 Mar;37(2-3):281-289.
    PMID: 39754505 DOI: 10.1177/10105395241311134
    Our study explored the link between body mass index (BMI) and symptoms of depression, anxiety, and suicidality among university students in China. We conducted a cross-sectional study involving 11 251 undergraduates from seven provinces. Body mass index, physical health status, economic status, depression and anxiety symptoms, and suicidality were self-reported using validated questionnaires. Multiple logistic regression models were fitted for males and females. Results showed that of 11 251 participants (mean age = 20.7 ± 1.3), 22.6% were with underweight, 6.1% with overweight, and 2.7% with obesity. Females with class III obesity had higher suicide risk (adjusted odds ratio [aOR] = 2.05) and suicide attempt history (aOR = 3.55). Males with overweight and class I obesity had lower odds of depressive symptoms. Suicide attempt history was associated with higher odds of overweight for males (aOR = 2.58). Clinicians and public health authorities should take into consideration the implications of overweight and obesity levels on suicidality.
    MeSH terms: Adult; China/epidemiology; Cross-Sectional Studies; Female; Humans; Male; Surveys and Questionnaires; Risk Factors; Students/psychology; Students/statistics & numerical data; Suicide, Attempted/psychology; Suicide, Attempted/statistics & numerical data; Thinness/epidemiology; Thinness/psychology; Body Mass Index*; Young Adult
  7. Ishak NA, Serri NA, Samsudin H, Murad M
    J Food Sci, 2025 Apr;90(4):e70177.
    PMID: 40183783 DOI: 10.1111/1750-3841.70177
    Papaya fruit, which has a medium level of pectin content, faces critical challenges in the production of papaya juice, leading to an undesirable, highly viscous texture. Conventional reliance on the use of free pectinase enzymes has addressed this issue; however, their single-use nature has limited their efficiency and has subsequently brought about an increase in processing costs. Hence, immobilized pectinase-alginate (IPA) beads with calcium chloride were developed, and the effects of their reusability on the physicochemical and antioxidant characteristics of papaya juice were analyzed. Immobilization of pectinase enzyme with sodium alginate (2%-4%) and calcium chloride (0.1-0.3 M) resulted in an immobilization yield ranging from 93.06% to 95.82%. It was found that the IPA beads maintained more than 80% relative activity even after seven subsequent cycles while demonstrating storage stability with more than 88% residual activity for up to 35 days, proving their efficiency and sustainability. In the first cycle, treatment with IPA beads significantly reduced the pectin content (51.61 mg GalAE/100 mL) and viscosity (1.15 mPa.s) of papaya juice compared to the control, which exhibited higher values of 235.00 mg GalAE/100 mL and 2.46 mPa.s, respectively. Both immobilized and free enzymes exhibit comparable results, outperforming the control, but IPA benefits by improving process efficiency through enzyme reusability. In addition, IPA could significantly maintain papaya juice's antioxidant activity over four treatment cycles. Future research is needed to focus on industrial scaling, shelf life of the IPA beads, and consumer acceptance of the papaya juice treated with immobilized enzyme. PRACTICAL APPLICATION: Papaya juice processing is challenging due to its moderate pectin content, which contributes to high viscosity, reduced yield, and increased turbidity, affecting overall juice quality. The conventional application of free pectinase in juice processing introduces further complications related to enzyme stability, cost, and recovery for reuse. Immobilizing pectinase minimizes waste via enzyme reuse, decreases juice viscosity, enhances clarity, and maintains antioxidant properties, thereby promoting process sustainability and meeting consumer preferences. The technology can be scaled and applied to a wide range of fruit juice processing industries, improving efficiency and nutritional value.
    MeSH terms: Fruit/chemistry; Pectins/analysis; Viscosity
  8. Li J, Beh WF, Wang IT, Wu Y
    Medicine (Baltimore), 2025 Jan 03;104(1):e41224.
    PMID: 40184117 DOI: 10.1097/MD.0000000000041224
    BACKGROUND: The prevalence rates of depression and anxiety are constantly increasing, and the aging population is growing in China. Music-based intervention is a nonpharmacological treatment that is used to treat depression and anxiety and that has very few side effects. The aim of this study was to explore the effect of Chinese 5-element music-based intervention (CFEMBI) based on the midnight-noon ebb-flow theory on depression and anxiety among older adults.

    METHODS: A total of 86 patients were divided into the control and experimental groups. The control group underwent a traditional CFEMBI, and the experimental group underwent a CFEMBI based on the midnight-noon ebb-flow theory. Outcomes were assessed at the 4th week, 8th week, and 12th week during the intervention.

    RESULTS: After the 12th week, there was a significant difference in scores on the Hamilton Depression Rating Scale (HAM-D) between the control and experimental groups (Z = -6.375, P value 

    MeSH terms: Aged; China; Female; Humans; Male; Middle Aged; Personal Satisfaction; Psychiatric Status Rating Scales; Treatment Outcome
  9. Mizan NS, Al-Talib H, Wang SM
    JMIR Res Protoc, 2025 Apr 04;14:e66350.
    PMID: 40184188 DOI: 10.2196/66350
    BACKGROUND: Intestinal protozoal infections caused by Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum are prevalent in Malaysia. They cause severe diarrheal diseases with symptoms such as bloody stools, abdominal pain, stomach discomfort, and bloating. These infection outbreaks have been reported in diverse socioeconomic backgrounds and geographical regions usually during the rainy season or in areas with poor sanitation. Despite the importance of these infections, data on its overall prevalence, risk factors, and diagnostic methods remain limited.

    OBJECTIVE: The aim of this study is to systematically review and synthesize evidence on the risk factors, prevalence, and detection methods for intestinal protozoal infections in Malaysia, offering insights that are applicable to other tropical and low-income regions.

    METHODS: Studies on intestinal protozoal infections among Malaysian patients published after January 2010 up to November 2024 will be eligible for inclusion. The eligibility criteria include studies investigating infections caused by E. histolytica, G. lamblia, and C. parvum using validated diagnostic methods such as microscopy, molecular techniques, or immunoassays. Case reports, reviews, and studies without original data will be excluded. Comprehensive database searches will be conducted in PubMed/MEDLINE, Scopus, ProQuest, Web of Science, Google Scholar, and the Cochrane Library. The reference lists of selected papers are also checked. A standardized data extraction form will be used to record study characteristics, outcomes, and associated variables. Risk of bias will be assessed using the Joanna Briggs Institute tools and Newcastle-Ottawa Scale approach. Data synthesis will utilize a random effects model to estimate pooled prevalence and identify risk factors associated with these infections. Subgroup analyses will examine variations by geographic region and diagnostic method. Statistical heterogeneity will be assessed using I2 statistic and meta-regression. Publication bias will be assessed using Egger and Begg funnel plot test. The results are reported in accordance with PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines.

    RESULTS: This systematic review was funded in June 2024. Database searches were started in July 2024, and we identified 1652 papers as of December 2024 for screening. Completion of study screening is anticipated by May 2025, with data extraction and analysis expected to conclude by December 2025.

    CONCLUSIONS: Our study will address critical knowledge gaps in the epidemiology and risk factors of intestinal protozoal infections in Malaysia. Study limitations include potential bias in study selection, heterogeneity in diagnostic methods, and differences in the reporting quality of the included studies. Our findings will provide valuable insights into the prevalence of these infections, the associated risk factors, and the diagnostic techniques employed, which should strengthen public health measures, improve diagnostic procedures, and guide future research to reduce the prevalence of intestinal protozoal infections in Malaysia.

    TRIAL REGISTRATION: PROSPERO (International Prospective Registry of Systematic Reviews) registration CRD42023456199; https://www.crd.york.ac.uk/PROSPERO/view/CRD42023456199.

    INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): DERR1-10.2196/66350.

    MeSH terms: Humans; Malaysia/epidemiology; Risk Factors; Meta-Analysis as Topic; Prevalence; Giardia lamblia
  10. Abraham P, McMullin C, William T, Rajahram GS, Jelip J, Teo R, et al.
    PLoS Negl Trop Dis, 2025 Apr;19(4):e0012180.
    PMID: 40184425 DOI: 10.1371/journal.pntd.0012180
    BACKGROUND: Malaysia has free universal access to malaria care; however, out-of-pocket costs are unknown. This study estimated and compared household costs of illness during a unique time when four species of malaria were present, due to the emergence of zoonotic Plasmodium knowlesi during the elimination phase of non-zoonotic species in Sabah, Malaysia.

    METHODOLOGY/PRINCIPAL FINDINGS: Household costs were estimated from patient-level surveys collected from four hospitals between 2013 and 2016. Direct costs including medical and associated travel costs, and indirect costs due to lost productivity were included. One hundred and fifty-two malaria cases were enrolled: P. knowlesi (n=108), P. vivax (n=22), P. falciparum (n=16), and P. malariae (n=6). Costs were inflated to 2023 Malaysian Ringgits and reported in United States dollars (US$). Across all cases, the mean total costs were US$131 (SD=102), with productivity losses accounting for 58% of costs (US$76; SD=70). P. vivax had the highest mean total household cost at US$199 (SD=174), followed by P. knowlesi and P. falciparum at US$119 (SD=81 and SD=83, respectively), and P. malariae (US$99; SD=42). Most patients (80%) experienced direct health costs above 10% of monthly income, with 58 (38%) patients experiencing health spending over 25% of monthly income, consistent with catastrophic health expenditure.

    CONCLUSIONS/SIGNIFICANCE: Despite Malaysia's free health-system care for malaria, patients and families face other related medical, travel, and indirect costs. Household out-of-pocket costs were driven by productivity losses; primarily attributed to infections in working-aged males in rural agricultural-based occupations. Costs for P. vivax were higher than those of P. knowlesi and P. falciparum. This may be attributable to a younger age profile and the longer treatment required to clear the liver-stage parasites of P. vivax.

    MeSH terms: Adolescent; Adult; Aged; Animals; Child; Child, Preschool; Health Expenditures; Family Characteristics; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Plasmodium falciparum/isolation & purification; Plasmodium malariae/isolation & purification; Plasmodium vivax; Travel/economics; Plasmodium knowlesi/isolation & purification; Cost of Illness*; Young Adult
  11. Martin DM, Alduraywish A, Bakir AA, Dokos PhD S, Bakouri M, Bai S, et al.
    J ECT, 2025 Apr 03.
    PMID: 40184538 DOI: 10.1097/YCT.0000000000001135
    OBJECTIVES: Modification of the electrode placement with electroconvulsive therapy (ECT) impacts on patient outcomes, including cognitive effects. The investigation of alternative right unilateral placements (RUL) has the potential to further improve ECT outcomes by reducing cognitive side effects.

    METHODS: This study investigated differences in the intracerebral distribution of current density comparing the standard d'Elia right unilateral positioning (temporoparietal [TP]) with two recently developed RUL placements, focal electrically administered seizure therapy (FEAST) and frontoparietal (FP) stimulation. The strength of the electrical fields (E-fields) was compared in brain regions of interest (ROIs) implicated in both clinical and cognitive outcomes. Computational modeling was conducted in the head models of five participants with major depression who had received RUL ECT.

    RESULTS: Results showed that the FEAST and FP placements were associated with significantly less bilateral stimulation effects in regions hypothesized to be associated with memory, whereas the FP and TP placements produced significantly greater bilateral stimulation effects in some regions hypothesized to be associated with efficacy. FEAST, using a smaller anterior electrode, produced significantly greater E-fields in some ROIs associated with memory and efficacy, although those differences were much smaller in magnitude.

    CONCLUSIONS: These findings suggest that both FEAST and FP may result in a superior clinical profile relative to TP. Future clinical studies are required to confirm the differences in clinical outcomes associated with the novel RUL placements.

  12. Bolt EET, Chee SY, van der Cingel M
    J Adv Nurs, 2025 May;81(5):2617-2630.
    PMID: 39356035 DOI: 10.1111/jan.16506
    AIMS: To explore and describe the meaning of nurses working in care for older adults give to the nursing professional identity.

    DESIGN: A qualitative approach was taken.

    METHODS: Semi-structured interviews were conducted with 50 bachelor and vocational-educated nurses working in care for older adults. Interviews were conducted between December 2019 and May 2020. Data were analysed and interpreted through inductive content analysis.

    RESULTS: Five themes embody the meaning of the nursing professional identity of nurses who work in care for older adults. The five themes are: born to care: a lifelong motivation to nursing; nursing through the noise: dedication in a demanding profession; the silent backbone: caught in the crossfire of interdisciplinary teams; learning under pressure: the demand for expanded nursing expertise and against the current: the barriers to advocacy in nursing.

    CONCLUSION: The professional nursing identity of nurses working in care for older adults is multi-faceted. A personal dedication to patient care, where patients 'human' aspect is heavily valued, commits nurses to their profession and underscores their dedication to upholding the quality standard in nursing practice.

    IMPLICATIONS FOR THE PROFESSION: The older adults' nursing identity highlights that nursing deserves acknowledgement as a professional occupation. Nurses should speak to the public about their professional roles to improve the public view of older adult nursing.

    IMPACT: A clear understanding of the older adult nursing professional identity clarifies specific roles, experiences and expectations. This can help attract and retain nurses whose views of older adult nursing align with the nursing professional identity. This could help resolve nurse turnover and reduce shortages in older adult care.

    REPORTING METHOD: We adhered to Consolidated Criteria For Reporting Qualitative Research guidelines.

    PATIENT OR PUBLIC CONTRIBUTION: No patient or public contribution.

    MeSH terms: Adult; Aged; Attitude of Health Personnel; Female; Geriatric Nursing*; Humans; Male; Middle Aged; Social Identification*; Qualitative Research
  13. Otani K, Hang DV, Pittayanon R, Liu H, Chuah KH, Hsiang J, et al.
    J Gastroenterol Hepatol, 2025 Apr;40(4):832-843.
    PMID: 39726163 DOI: 10.1111/jgh.16862
    BACKGROUND AND AIM: Gastric cancer (GC)-related incidence and mortality rates remain high owing to Helicobacter pylori infection in Asia, and the importance of primary and secondary prevention of GC has been well recognized. We aimed to investigate the extent of overall agreement among clinicians in the Asia-Pacific region regarding the management of H. pylori infection.

    METHODS: The Upper Gastrointestinal (GI) Focus Group of the Asian Pacific Association of Gastroenterology-Emerging Leaders Committee developed an international survey, which was distributed to 98 clinicians in the Asia-Pacific region, compromising an online questionnaire focusing on the management of H. pylori infection.

    RESULTS: Participants responded from Japan (15, 15.3%), Hong Kong (15, 15.3%), Thailand (33, 33.7%), Vietnam (23, 23.5%), Malaysia (4, 4.1%), Singapore (3, 3.1%), and others (5, 5.1%). The most common first-line eradication regimen was clarithromycin (CAM) triple therapy, including proton pump inhibitor (PPI), amoxicillin (AMPC), and CAM (64.3%) for 14 days (70.4%). The most common second-line eradication regimen was levofloxacin (LVX) triple therapy, including PPI, AMPC, and LVX (22.4%) for 14 days (67.3%). Eradication therapy was deemed necessary for all asymptomatic adults and minors (aged ≤ 17 years) currently infected with H. pylori by 81.6% and 64.3% of respondents, respectively, with 82.7% considering upper GI endoscopy for GC screening useful in the secondary prevention of GC.

    CONCLUSION: There appears to be a growing consensus among clinicians, acknowledging the necessity of eradication therapy. We anticipate that this study will establish a new benchmark in preventive medicine aimed at eradicating GC in the Asia-Pacific region.

    MeSH terms: Adult; Amoxicillin/administration & dosage; Amoxicillin/therapeutic use; Asia/epidemiology; Drug Therapy, Combination; Female; Humans; Male; Middle Aged; Surveys and Questionnaires; Stomach Neoplasms/prevention & control; Helicobacter pylori*; Clarithromycin/administration & dosage; Clarithromycin/therapeutic use; Proton Pump Inhibitors/administration & dosage; Proton Pump Inhibitors/therapeutic use; Levofloxacin/administration & dosage
  14. Woon LS, Bastiampillai T, Looi JCL
    Aust Health Rev, 2025 Apr;49.
    PMID: 40164090 DOI: 10.1071/AH25011
    Objective The Australian Medicare Benefits Schedule (MBS) telehealth items were expanded in March 2020 and consolidated in January 2022. We aim to describe the trend of the ratio of MBS psychiatric video items for once-off assessments to follow-up sessions and examine the effect of telehealth consolidation. Methods Psychiatric once-off item to follow-up item ratios in face-to-face (F2F) and video modes were computed from state and territory-level monthly Medicare data (January 2016-December 2023). We performed a multilevel interrupted times series analysis (ITSA) of once-off video consultations with telehealth consolidation as the intervention, followed by subgroup analyses by age groups. Finally, in the young adult group (25-44years), we performed a multiple-group ITSA comparing video once-off to follow-up ratios between genders. Results The once-off to follow-up ratio for video items after telehealth consolidation greatly increased compared with F2F items, with rapid growth in once-off assessments. Sustained high video once-off assessment usage occurred in the youth (15-24years) and young adult (25-44years) groups, while other age groups showed declines following the initial rise. The male group showed a greater initial increase in the video once-off to follow-up ratio among young adults but the ratio continued to rise only in the female group. Conclusions The evolving trends of reimbursed telepsychiatry consultations require further examination given their potential implications concerning service quality, health equity, health attitudes and behaviours, and healthcare costs.
    MeSH terms: Adolescent; Adult; Aged; Australia; Female; Humans; Male; Middle Aged; National Health Programs*; Young Adult; Interrupted Time Series Analysis
  15. Bakar KA, Feroz SR
    J Biomol Struct Dyn, 2025 Apr 03.
    PMID: 40177920 DOI: 10.1080/07391102.2025.2483314
    Mitragynine (MTG), the primary psychoactive alkaloid in Mitragyna speciosa (kratom), has garnered much attention for its therapeutic properties, which is attributed mainly to its selective action on opioid receptors. Despite its clinical potential, the molecular framework of its binding to plasma proteins remains incomplete. Specifically, no studies have thoroughly examined its interaction with α1-acid glycoprotein (AAG), a carrier protein in the circulatory system that influences drug disposition and bioavailability. Hence, this study aims to explore the binding dynamics between MTG and AAG using a combination of spectroscopic, calorimetric, microscopic, and computational methods. Based on isothermal titration calorimetric and fluorescence studies, an intermediate affinity for the MTG-AAG binding was determined (Ka ∼ 105 M-1). Despite evidence of microenvironmental changes around Trp residues, MTG binding did not disrupt the overall structural integrity of AAG. Thermodynamic analysis indicated that the MTG-AAG interaction was energetically favorable, and enthalpy driven mainly by hydrogen bonding and van der Waals forces, with negative entropy change suggesting a more ordered complex formation. Docking analysis showed MTG embedded more deeply within the central cavity of variant F1*S, enhancing complex stability, as opposed to binding near the cavity entrance in variant A. Molecular dynamics simulations supported the stable complexation of MTG with both AAG variants, with variant F1*S maintaining more structural compactness while variant A exhibited slight unfolding upon binding. These findings have clear significance on the potential therapeutic applications of kratom-derived drugs, especially those structurally related to MTG.
  16. Nasrudin N, Sazlina SG, Cheong AT, Lee PY, Teo SH, Aneesa AR, et al.
    JMIR Form Res, 2025 Mar 28;9:e65542.
    PMID: 40177938 DOI: 10.2196/65542
    BACKGROUND: Digital health interventions such as web health applications significantly enhance screening accessibility and uptake, particularly for individuals with low literacy and income levels. By involving stakeholders-including health care professionals, patients, and technical experts-an intervention can be tailored to effectively meet the users' needs, ensuring contextual relevance for better acceptance and impact.

    OBJECTIVE: The aim of this study is to prioritize the content and user interface appropriate for developing a web health application, known as the MAwar app, to promote breast and cervical cancer screening.

    METHODS: A cross-sectional study for stakeholder engagement was conducted to develop a web-based application known as the MAwar app as part of a larger study entitled "The Effectiveness of an Interactive Web Application to Motivate and Raise Awareness on Early Detection of Breast and Cervical Cancers (The MAwar study)". The stakeholder engagement process was conducted in a public health district that oversees 12 public primary care clinics with existing cervical and breast cancer screening programs. We purposively selected the stakeholders for their relevant roles in breast and cervical cancer screening (health care staff, patients, and public representatives), as well as expertise in software and user interface design (technology experts). The Quality Function Deployment method was used to reflect the priorities of diverse stakeholders (health care, technology experts, patients, and public representatives) in its design. The Quality Function Deployment method facilitated the translation of stakeholder perspectives into app features. Stakeholders rated features on a scale from 1 (least important) to 5 (most important), ensuring the app's design resonated with user needs. The correlations between the "WHATs" (user requirements) and the "HOWs" (technical requirements) were scored using a 3-point ordinal scale, with 1 indicating weak correlation, 5 indicating medium correlation, and 9 indicating the strongest correlation.

    RESULTS: A total of 13 stakeholders participated in the study, including women who had either underwent or never had health screening, a health administrator, a primary care physician, medical officers, nurses, and software designers. Stakeholder evaluations highlighted cost-free access (mean 4.64, SD 0.81), comprehensive cancer information (mean 4.55, SD 0.69), detailed screening benefits (mean 4.45, SD 0.68), detailed screening facilities (mean 4.45, SD 0.68) and personalized risk calculator for breast and cervical cancers (mean 4.45, SD 0.68) as essential priorities of the app. The highest-ranked features include detailed information on screening procedures (weighted score [WS]=367.84), information on treatment options (WS=345.80), benefits of screening (WS=333.75), information about breast and cervical cancers (WS=332.15), and frequently asked questions about the concerns around screening (WS=312.00).

    CONCLUSIONS: The MAwar app, conceived through a collaborative, stakeholder-driven process, represents a significant step in leveraging digital health solutions to tackle cancer screening disparities. By prioritizing accessibility, information quality, and clarity on benefits, the app promises to encourage early cancer detection and management for targeted communities.

    MeSH terms: Adult; Cross-Sectional Studies; Female; Humans; Middle Aged; Patient Acceptance of Health Care*; Internet; Mobile Applications*
  17. Mansori S, Tendulkar A, Rostamzadeh M
    Health Educ Behav, 2025 Apr 03.
    PMID: 40178144 DOI: 10.1177/10901981251328590
    Despite advancements in screening technologies, gynecologic cancers remain among the deadliest and most overlooked cancers. Early detection is crucial, but many women are reluctant to undergo screening due to various barriers and misconceptions. This study explores the motivations and barriers influencing women's decisions regarding early gynecologic cancer screening in Malaysia. In-depth interviews were conducted with 20 professional women from diverse backgrounds using a qualitative approach. Data were analyzed through open and axial coding to identify key themes. An empathy map and user journey map captured the psychological and behavioral aspects of decision-making. The study identified themes such as lack of knowledge, reliance on social media and health care professionals, financial and time constraints, fear of results, and cultural stigmas. Participants with higher awareness were more likely to undergo screening, while those with personal or family cancer experiences showed greater vigilance. The findings emphasize the importance of culturally sensitive communication and health care professionals' role in reducing stigma and promoting early screening. The study's insights contribute to developing strategies to encourage early detection and improve health outcomes for women. These strategies align with the United Nations' Sustainable Development Goals (SDGs), particularly SDG 3 (Good Health and Well-being) and SDG 5 (Gender Equality), promoting health equity and empowering women. As emphasized by SDG 17 (Partnerships for the Goals), achieving these goals requires strong partnerships and collaborative efforts to create a supportive environment for early cancer screening initiatives. Policymakers can use these insights to foster health equity and empower women through supportive screening environments.
  18. Mathijssen EG, Trappenburg JC, Alberts MJ, Balguid A, Dempsey RJ, Goyal M, et al.
    Eur Stroke J, 2025 Apr 03.
    PMID: 40178329 DOI: 10.1177/23969873251329841
    BACKGROUND: Despite international recognition of stroke as a significant health priority, discrepancies persist between the target values for stroke quality measures and the actual values that are achieved in clinical practice, referred to as gaps. This study aimed to reach consensus among international experts on prioritizing gaps in stroke care.

    METHODS: A two-round Delphi process was conducted, surveying an international expert panel in the field of stroke care and cerebrovascular medicine, including patient representatives, healthcare professionals, researchers, policymakers, and medical directors. Experts scored the importance and required effort to close 13 gaps throughout the stroke care continuum and proposed potential solutions. Data were analyzed using descriptive statistics and qualitative analysis methods.

    RESULTS: In the first and second Delphi rounds, 35 and 30 experts participated, respectively. Expert consensus was reached on the high importance of closing 11 out of 13 gaps. Two out of 13 gaps were considered moderately important to close, with expert consensus for one of these two gaps. Expert consensus indicated that only one gap, related to the prevention of complications after stroke, requires moderate effort to close, whereas the others were considered to require high effort to close. Key focus areas for potential solutions included: "Care infrastructure," "Geographic disparities," "Interdisciplinary collaboration," and "Advocacy and funding."

    CONCLUSIONS: While closing gaps in stroke care primarily requires high effort and substantial resources, targeted interventions in the identified key focus areas may provide feasible and clinically meaningful improvements.

  19. Kazim AR, Low VL, Subramaniam V, Houssaini J, Tappe D, Heo CC
    J Med Entomol, 2025 Apr 03.
    PMID: 40178432 DOI: 10.1093/jme/tjaf038
    A total of 68 adult hard ticks were recovered from 5 cases of infestation involving pet rabbits (Oryctolagus cuniculus domesticus) in Malaysia. The ticks were later identified as the tropical brown dog tick, Rhipicephalus linnaei (Audouin, 1826). Pathogen screening showed 2 out of the 68 Rh. linnaei ticks (2.94% infection rate) were positive for Rickettsia. BLAST analyses revealed that both samples were identical to Rickettsia conorii subsp. raoultii. This paper reports the first detection of R. conorii subsp. raoultii in Rh. linnaei, also highlighting the potential zoonotic threat posed by ticks infesting exotic pets such as rabbits.
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