Browse publications by year: 2025

  1. Zhang S, Song H, Chang M, Lu Y, Liu S, Wu J, et al.
    Int J Biol Macromol, 2025 Feb 12;304(Pt 2):140973.
    PMID: 39952536 DOI: 10.1016/j.ijbiomac.2025.140973
    Cisplatin (Cis) is among the most widely employed antitumour agents, although its clinical application is limited by self-induced multiple-organ toxicity. Previous studies have demonstrated the essential role of mitochondrial injury in the pathogenesis of Cis-induced ovotoxicity. Notably, mesenchymal stem cell-extracellular vesicles (MSC-EVs), potential cell-free therapeutic agents, exhibit pronounced advantages for the treatment of ovarian dysfunction. However, little is known about which core component contained in MSC-EVs plays a major role in repairing Cis-induced ovarian damage, and further, the potential mechanisms underlying the repair of mitochondrial damage remain unclear. Herein, our study first verified that MSC-EVs effectively ameliorate Cis-induced ovarian dysfunction by upregulating the level of mitochondrion-localized glutamic acid-rich protein (MGARP), after which MGARP repairs mitochondrial damage and inhibits cellular ROS production by combining with and suppressing the degradation of peroxiredoxin 2 (PRDX2) in granulosa cells (GCs). More importantly, our study further showed that heat shock protein family A member 8 (HSPA8) is indispensable for MenSC-EV-mediated improvement of Cis-induced ovotoxicity. This investigation provides novel insights into the molecular mechanisms by which MSCs alleviate Cis-induced ovotoxicity through improving mitochondrial dysfunction.
  2. Li D, Liang Y, Xia Q, Pan D, Du L, He J, et al.
    Food Microbiol, 2025 Jun;128:104715.
    PMID: 39952759 DOI: 10.1016/j.fm.2024.104715
    Yeast extracellular proteases play a key role in developing the taste of dry-cured ham, whereas the mechanism of yeast proteases on taste formation of dry-cured ham is not fully studied. The proteases characteristics of yeast isolated form Jinhua ham, hydrolysis capacities for myofibrillar proteins (MP), free amino acid contents, metabolite compositions, taste parameters and the relationship between metabolites and taste parameters were investigated to reveal the mechanism of Rhodotorula mucilaginosa AUMC 9298 (RM) and Candida parapsilosis d70a (CP) proteases on MP hydrolysis and taste development of dry-cured ham. The proteases of RM and CP showed high hydrolysis activities at the conditions of pH 5.0-8.0 and 30-50 °C. The proteases of RM showed higher capability to degrade myosin compared with CP proteases and Pichia kudriavzevii XS-5 (PK) proteases. The total free amino acid contents increased from 18.44 mg/100 mL in PK to 33.91 mg/100 mL in RM and 25.28 mg/100 mL in CP after 4 h hydrolysis of MP. Thirty-two metabolites were identified by LC-MS/MS, and peptides and amino acid derivatives were the key components of MP hydrolysates. The scores of umami, richness and aftertaste showed the largest values in RM among these groups. PLS-DA and correlation demonstrated that aspartic acid, N-Methyl-aspartic acid, Glu-Glu, γ-Glu-Cys, glutamic acid, γ-Glu-Glu and γ-Glu-Gln were positive correlation with the improvement of umami, richness and aftertaste.
    MeSH terms: Amino Acids/analysis; Amino Acids/metabolism; Animals; Candida/enzymology; Candida/growth & development; Candida/metabolism; Chromatography, Liquid; Fungal Proteins/metabolism; Humans; Hydrolysis; Meat Products/analysis; Meat Products/microbiology; Myofibrils/metabolism; Rhodotorula/enzymology; Rhodotorula/growth & development; Rhodotorula/metabolism; Swine; Taste*; Multivariate Analysis; Tandem Mass Spectrometry*; Proteolysis
  3. Wattanapisit A, Sirirak K, Wattanapisit S, Jenn Ng C, Hai Teo C, Wiwatkunupakarn N, et al.
    J Prim Care Community Health, 2025;16:21501319251320171.
    PMID: 39952911 DOI: 10.1177/21501319251320171
    INTRODUCTION: Family medicine embraces a wide range of principles. Identifying appropriate journals for publishing family medicine case reports can be challenging for authors. This study aims to identify journals related to family medicine that publish case reports and summarize the requirements of case report submissions.

    METHODS: Journals related to family medicine were identified from the subject categories: "family practice" in Scopus and "primary health care" in Web of Science. The author's instructions on the journal websites regarding the criteria for case report submissions were reviewed, and the specific requirements for case reports of each journal were recorded. Journals were excluded if family medicine was not the main aim and scope.

    RESULTS: Among the 80 journals focused on family medicine, 30 (37.5%) were found to accept submissions of case reports. These reports were classified into various article types, such as case reports, patient studies, clinical case studies. The length of the main text varied between 400 and 3000 words, and some journals did not mandate an abstract. However, for those that did, abstracts typically ranged from 50 to 300 words. The number of references cited ranged from 5 to 30.

    CONCLUSIONS: Less than half of the journals in the family medicine field accept case report submissions. It is advisable for authors to choose their target journal early in the preparation process, as each journal typically provides specific submission guidelines and instructions.

    MeSH terms: Family Practice*; Humans; Periodicals as Topic*; Publishing; Editorial Policies
  4. Abdel Jalil AA, Koo TH, Marshall JB, Fass R
    Dig Dis Sci, 2025 Feb 14.
    PMID: 39953187 DOI: 10.1007/s10620-025-08899-y
    BACKGROUND: There is scarce information on the state of general gastrointestinal (GI) fellow knowledge of esophageal motility and the interpretation skills of esophageal high-resolution manometry (HRM).

    AIM: This study aimed to assess GI fellow knowledge of esophageal motility and manometry interpretation.

    METHODS: A 6-month educational program consisting of eight didactic sessions in the form of a weekly educational email, three didactic conferences, and handouts pertaining to the Chicago Classification (v3.0) of esophageal HRM was conducted. Both pre- and post-intervention surveys were collected using SurveyMonkey®. Five questions assessed fellows' knowledge of esophageal motility and manometry, and two questions examined their self-assessment of knowledge and confidence in managing esophageal motility disorders (EMDs). Descriptive statistics and Student's t test were used for the analysis.

    RESULTS: Ten GI fellows (four first-year, five second-year, and one third-year) from a single academic institution participated in the intervention. Fellows showed a trend for better knowledge of the clinical aspects of esophageal motility over HRM interpretation (P value 0.09). On a scale of 1-5, with 5 being the highest, fellows' self-assessment of esophageal motility knowledge pre-intervention averaged 1.8 (SD 0.78) and post-intervention 2.9 (SD 0.99); P value 0.007. Fellows' confidence in managing EMDs pre-intervention averaged 1.7 (SD 0.66) and post-intervention 2.8 (SD 0.91); P value 0.04. Subgroup analyses, including fellows' self-assessment of knowledge, and fellows' confidence in managing EMDs, maintained statistically significance for level of training.

    CONCLUSION: GI fellow knowledge of esophageal motility and manometry interpretation, as well as confidence in managing EMDs, improved significantly after a 6-month formal educational program.

  5. McHugh N, Lyons RE, Keogh I, Flaherty GT
    Trop Dis Travel Med Vaccines, 2025 Feb 15;11(1):3.
    PMID: 39953586 DOI: 10.1186/s40794-024-00238-9
    BACKGROUND: Disorders of the ear, nose and throat may be the most commonly occurring pre-existing health condition in international travellers. Despite their high incidence, there is limited guidance for travellers and their clinicians on their prevention and management. This narrative review addresses this deficit by compiling and discussing available evidence on this neglected subject.

    METHODS: A comprehensive review of the literature was undertaken using Medine and Scopus databases and multiple combinations of relevant MeSH search terms. Further references were obtained from focused searches on specific issues and manual review of the reference lists of articles obtained from the primary search.

    RESULTS: Nasal congestion or discharge are among the most common complaints amongst travellers and various causes are reviewed. Changes in elevation result in a pressure differential between the atmospheric pressure and the middle ear and paranasal sinuses. The effects of air travel, recreational high-altitude exposure and diving are considered. Various causes of epistaxis in travellers such as cold air exposure and recreational cocaine use are discussed. The aetiology of a discharging ear in travellers includes otitis externa. The most frequently described travel-specific aetiology of dizziness is motion sickness while mal de debarquement is a specific subtype which affects travellers and is most commonly associated with sea travel. Surgical tourism for treatment of ear, nose and throat pathology is well established and various precautions are presented for post-operative travel. Obstructive sleep apnoea is discussed from the perspective of international travel. The challenges facing travellers with hearing impairment are explored.

    CONCLUSIONS: This review critically discusses the prevention, diagnosis, and management of acute and chronic ENT conditions in the travelling population. Several areas of inquiry are highlighted that require further investigation. Close communication between ENT specialists and travel medicine practitioners is recommended in the preparation of selected patients for international travel.

  6. Godina E, Khromov-Borisov N, Bondareva E
    J Physiol Anthropol, 2025 Feb 14;44(1):6.
    PMID: 39953630 DOI: 10.1186/s40101-025-00386-7
    BACKGROUND: Prediction of sports success (sports talent) based on individual genetic characteristics is the main goal of sports genetics/genomics. Most often, markers of predisposition to speed-strength sports, or endurance, are single-nucleotide variants in various parts of DNA. One of the most studied variants is the C/T variant in the ACTN3 gene. The accumulated data on the association of this variant with success in various sports is sufficient to conduct a meta-analysis. The purpose of the present review is to analyze the prognostic utility of the data presented in the literature on molecular genetic markers of genetic predisposition to achieve outstanding sports results using the example of the C > T variant of ACTN3 (rs1815739).

    MAIN BODY: A total of 42 studies were included in the analysis, with a total number of 41,054 individuals (of which 10,442 were in the athlete group and 30,612 in the control group). For each study included in the analysis, the agreement of genotype frequencies with Hardy-Weinberg equilibrium was tested, as well as the presence of an excess or deficit of heterozygotes. Prediction intervals for the overall effect size (OR-odds ratio) was estimated. Both in the subgroups of athletes and controls, a significant difference FIS from zero was found, suggesting inbreeding or outbreeding, as well as a very wide 95% CI for FIS. A meta-analysis was conducted for dominant, codominant, and recessive inheritance models. The obtained ORs and their 95% CIs were in the range of almost negligible values or have very wide CIs. The evaluation for the recessive model showed 95% PI for the OR lies between 0.74 to 1.92. Statistically, it does not differ from zero, which means that in some 95% of studies comparable to those in the analysis, the true effect size will fall in this interval.

    CONCLUSION: Despite numerous attempts to identify genetic variants associated with success in elite sports, progress in this direction remains insignificant. Thus, no sports or sports roles were found for which the C > T variant of the ACTN3 gene would be a reliable prognostic marker for assessing an individual predisposition to achieve high sports performance. The results of the present meta-analysis support the conclusion that neutral gene polymorphism-from evolutionary or adaptive point of view-is not a trait that can be selected or used as a predictive tool in sports.

    MeSH terms: Humans; Polymorphism, Single Nucleotide/genetics; Athletes
  7. Abbasian Ardakani A, Mohammadi A, Yeong CH, Ng WL, Ng AH, Tangaraju KN, et al.
    J Ultrasound Med, 2025 Feb 15.
    PMID: 39953921 DOI: 10.1002/jum.16665
    OBJECTIVE: To develop, test, and externally validate a hybrid artificial intelligence (AI) model based on hand-crafted and deep radiomics features extracted from B-mode ultrasound images in differentiating benign and malignant thyroid nodules compared to senior and junior radiologists.

    METHODS: A total of 1602 thyroid nodules from four centers across two countries (Iran and Malaysia) were included for the development and validation of AI models. From each original and expanded contour, which included the peritumoral region, 2060 handcrafted and 1024 deep radiomics features were extracted to assess the effectiveness of the peritumoral region in the AI diagnosis profile. The performance of four algorithms, namely, support vector machine with linear (SVM_lin) and radial basis function (SVM_RBF) kernels, logistic regression, and K-nearest neighbor, was evaluated. The diagnostic performance of the proposed AI model was compared with two radiologists based on the American Thyroid Association (ATA) and the Thyroid Imaging Reporting & Data System (TI-RADS™) guidelines to show the model's applicability in clinical routines.

    RESULTS: Thirty-five hand-crafted and 36 deep radiomics features were considered for model development. In the training step, SVM_RBF and SVM_lin showed the best results when rectangular contours 40% greater than the original contours were used for both hand-crafted and deep features. Ensemble-learning with SVM_RBF and SVM_lin obtained AUC of 0.954, 0.949, 0.932, and 0.921 in internal and external validations of the Iran cohort and Malaysia cohorts 1 and 2, respectively, and outperformed both radiologists.

    CONCLUSION: The proposed AI model trained on nodule+the peripheral region performed optimally in external validations and outperformed the radiologists using the ATA and TI-RADS guidelines.

  8. Abulehia H, Mohd Nor NS, Sheikh Abdul Kadir SH
    Birth Defects Res, 2025 Feb;117(2):e2450.
    PMID: 39953946 DOI: 10.1002/bdr2.2450
    BACKGROUND: Bisphenol A (BPA) is a ubiquitous industrial compound found in an enormous variety of consumer goods such as plastics, epoxy resins, and thermal paper. Despite its widespread usage, the effects of BPA on the risk and development of metabolic syndrome are not fully understood. Prenatal exposure to BPA has been shown to disrupt the development of offspring's metabolically active tissues and increase their susceptibility to the adverse consequences of a trans-fat diet (TFD).

    METHODS: In this experiment, pregnant rats (n = 18) were randomly separated into three groups. These groups were treated from pregnancy day (PD) 2 to PD 21. Subsequently, the male offspring of these rats were provided either a normal-diet (ND) or a TFD from 3rd postnatal week (PNW) to 14th PNW. Then, protein expression of PPAR-γ and global DNA methylation were assessed in the adult rat offspring that were exposed to in utero BPA and subjected to postnatal TFD intake.

    RESULTS: The study findings have shown that there was no association between prenatal exposure to BPA and/or TFD consumption and PPAR-γ protein expression within all the study groups in the liver tissue. On the other hand, changes at the molecular level, as reflected by the global DNA hypermethylation induced by prenatal BPA and postnatal TFD intake in adult male SD rat offspring (PNW 14).

    CONCLUSIONS: This study underscores the potential impact of prenatal BPA exposure and postnatal TFD intake on epigenetic regulation, as evidenced by global DNA hypermethylation, despite no observable changes in PPAR-γ protein expression. These findings suggest that early-life environmental exposures may predispose individuals to metabolic disruptions, including diabetes and obesity, in adulthood or future generations.

    MeSH terms: Animals; Female; Liver/drug effects; Liver/metabolism; Male; Pregnancy; Maternal Exposure/adverse effects; Rats
  9. Habibi N, Mustafa AS, Nasser K, Al-Obaid I, Alfouzan W, Uddin S, et al.
    Mol Biol Rep, 2025 Feb 15;52(1):233.
    PMID: 39954144 DOI: 10.1007/s11033-025-10353-1
    BACKGROUND: Acinetobacter baumannii is a notorious nosocomial pathogen universally in healthcare settings. Its natural competent characteristics for genetic recombination are responsible for acquired antibiotic resistance and render it untreatable through commonly used antibiotics. Hence, characterizing the A. baumannii genomes for multidrug resistance carriage is of paramount importance. The study aimed to characterize the whole genome of clinical isolates of A. baumannii to identify specifically the types of antibiotic resistance genes, drug classes and mobile genetic elements. We also aimed to determine the significant multi-locus sequence tags (MLSTs). The phylogeny of the isolates was established with other clinical strains distributed globally.

    METHODS AND RESULTS: Fifteen clinical isolates (isolated from tracheal secretion, urine and bronchoalveolar lavage) were subjected to whole genome sequencing. Raw sequences were assembled using SPAdes and species were identified using KmerFinder 3.2. The assembled genomes were annotated using the Prokka v1.14.6. Resfinder 4.6.0 was used to determine antibiotic resistance genes. The sequences were aligned against seven housekeeping genes aka sequence tags (STs) available within the MLST database (v 2.0.9). MobileGeneticElement finder (v1.0.3) were used for profiling mobile genetic elements associated with the antibiotic resistance genes. The genomes of nosocomial A. baumannii were assembled with an average N50 of 23,480 and GC content of 38%. There were approximately 3700 CDs, 53 tRNA and 3 rRNA. About 80% of the isolates were ST2 type. The genomes possessed antibiotic resistance genes (n = 24) belonging to 17 drug classes. The predicted phenotype was multidrug resistant. Among the mobile genetic elements, 12 insertion sequences and 2 composite transposons were also found. The mode of antibiotic resistance was mostly through antibiotic inactivation in all the isolates.

    CONCLUSIONS: The results imply the occurrence of multidrug resistant genes in clinical isolates of A. baumannii strains in the healthcare settings of Kuwait. A more comprehensive survey should be undertaken for antimicrobial resistance monitoring on a regular basis for surveillance, contact tracing, and potential mitigation in clinical settings.

    MeSH terms: Humans; Microbial Sensitivity Tests; Phylogeny*; Genomics/methods
  10. Ng CM, Cheong WL, Chong CW, Teoh SL, Yap WS, Lee SWH
    Diabetes Metab Syndr, 2025 Feb 07;19(2):103206.
    PMID: 39954567 DOI: 10.1016/j.dsx.2025.103206
  11. Rani S, Ahmed MK, Keliang C, Hossain KB, Yusuf AHM, Chowdhury KMA, et al.
    Mar Pollut Bull, 2025 Feb 14;213:117675.
    PMID: 39954594 DOI: 10.1016/j.marpolbul.2025.117675
    Coastal areas serve as vital ecosystems that provide habitats for various organisms but are increasingly threatened by trace element pollution. This study examines trace element concentrations in water, fish, and shellfish from estuarine and coastal areas of Bangladesh's East Coast, evaluating their associated human health risks. The mean concentrations of all trace elements in surface waters of the East Coast were in the following descending order: Zn > Cu > As > Pb > Cr > Cd and all of the values were much higher than the guideline values. The mean concentrations for As, Pb, Cd, Cr, Ni, Cu and Zn for fish and shellfish were 0.42, 2.18, 0.6, 2.16, 0.68, 5.19 and 84.6 μg/g, respectively. Total value of estimated daily intake (EDI) for As, Pb, Cd, Cr, Cu and Zn were 3.108, 2.294, 0.874, 0.750, 5.856 and 31.36 mg/day, respectively. Most EDIs, except for As and Cu, were below tolerable limits. Total value of target hazard quotient (THQ) for As, Pb, Cd, Cr, Cu and Zn were 172.67, 10.924, 4.856, 0.0083, 2.4401 and 1.7421, respectively that suggested a higher level of potential non-carcinogenic health risk since THQ value is >1 except Cr. The target cancer risk (TR) value of As, Pb, Cd and Cr are ranged from 1.12E-04 to 1.39E-02, 1.87E-06 to 1.13E-04, 6.83E-05 to 1.13E-03 and 6.49E-05 to 1.05E-03, respectively. The TR values of As, Cr, and Cd in most studied fish exceeded the reference value (10-4), indicating a potential lifetime cancer risk for consumers. Immediate environmental interventions are essential to mitigate pollution and achieve sustainable development goals.
  12. Ang CYS, Nor MBM, Nordin NS, Kyi TZ, Razali A, Chiew YS
    Comput Methods Programs Biomed, 2025 Feb 09;262:108657.
    PMID: 39954654 DOI: 10.1016/j.cmpb.2025.108657
    BACKGROUND: Accurate estimation of resting energy expenditure (REE) is critical for guiding nutritional therapy in critically ill patients. While indirect calorimetry (IC) is the gold standard for REE measurement, it is not routinely feasible in clinical settings due to its complexity and cost. Predictive equations (PEs) offer a simpler alternative but are often inaccurate in critically ill populations. While recent advancements in machine learning (ML) and deep learning (DL) offer potential for improving REE estimation by capturing complex relationships between physiological variables, these approaches have not yet been widely applied or validated in critically ill populations.

    METHODOLOGY: This prospective study compared the performance of nine commonly used PEs, including the Harris-Benedict (H-B1919), Penn State, and TAH equations, with ML models (XGBoost, Random Forest Regressor [RFR], Support Vector Regression), and DL models (Convolutional Neural Networks [CNN]) in estimating REE in critically ill patients. A dataset of 300 IC measurements from an intensive care unit (ICU) was used, with REE measured by both IC and PEs. The ML/DL models were trained using a combination of static (i.e., age, height, body weight) and dynamic (i.e., minute ventilation, body temperature) variables. A five-fold cross validation was performed to assess the model prediction performance using the root mean square error (RMSE) metric.

    RESULTS: Of the PEs analysed, H-B1919 yielded the lowest RMSE at 362 calories. However, the XGBoost and RFR models significantly outperformed all PEs, achieving RMSE values of 199 and 200 calories, respectively. The CNN model demonstrated the poorest performance among ML models, with an RMSE of 250 calories. The inclusion of additional categorical variables such as body mass index (BMI) and body temperature classes slightly reduced RMSE across ML and DL models. Despite data augmentation and imputation techniques, no significant improvements in model performance were observed.

    CONCLUSION: ML models, particularly XGBoost and RFR, provide more accurate REE estimations than traditional PEs, highlighting their potential to better capture the complex, non-linear relationships between physiological variables and REE. These models offer a promising alternative for guiding nutritional therapy in clinical settings, though further validation on independent datasets and across diverse patient populations is warranted.

  13. Gan Y, Lee CM, Pairan S, Ahmad RARRL, Achok HN
    Clin Med (Lond), 2025 Feb 13.
    PMID: 39955071 DOI: 10.1016/j.clinme.2025.100294
    Thiamine (Vitamin B1) deficiency may present with diverse symptoms and is often triggered by chronic alcoholism, severe malnutrition, or hyperemesis gravidarum. While typically diagnosed clinically, atypical presentations may delay recognition and treatment. We report a 26-year-old pregnant woman in her second trimester with prolonged nausea and vomiting since early pregnancy. She developed ascending lower limb paralysis, dysarthria, horizontal nystagmus, and lagophthalmos. Initially managed as hypokalaemia periodic paralysis with thyrotoxicosis and later Guillain-Barré Syndrome (GBS), her symptoms worsened despite treatment. Subsequent MRI brain imaging revealed findings indicative of Wernicke encephalopathy, prompting high-dose thiamine therapy. This resulted in significant neurological improvement. This case underscores the importance of considering thiamine deficiency in atypical neurological presentations, particularly in pregnancy. Early recognition and prompt treatment can mitigate irreversible neurological damage, emphasizing the necessity of maintaining a high index of suspicion in clinical practice.
  14. Arai H, Ng CG, Siew WH, Abousheishaa AA
    Arch Psychiatr Nurs, 2025 Feb;54:102-109.
    PMID: 39955138 DOI: 10.1016/j.apnu.2025.01.006
    INTRODUCTION: Few studies have applied Benner's nursing theory in evaluating psychiatric nursing care for patients with schizophrenia and the complication of cancer. Further study and training are needed in the management of these diseases.

    OBJECTIVES: (1) To elucidate and categorize psychiatric nursing expertise in schizophrenia and cancer according to the five stages of Benner's nursing theory. (2) To identify stage-specific learning needs for the psychiatric nursing care of schizophrenia and cancer, and to propose tailored educational programs. (3) To clarify the differences in the roles and training of psychiatric nurses in Malaysia and Japan.

    METHODS: A qualitative descriptive design was adopted. Semi-structured interviews were conducted with a total of 20 psychiatric nurses in Malaysia and Japan. The data were thematically analyzed and categorized with Benner's theory.

    RESULTS: Benner's five stages of proficiency were: Novices followed pre-established routines; advanced beginners focused on psychiatric symptoms and behavior; competent nurses determined and prioritized methods of care; proficient nurses flexibly adjusted care to the patient's condition; and experts lent extensive experience to the team and patients. The following learning needs were identified: Novices struggled with identifying physical and psychiatric symptoms; advanced beginners had difficulties understanding ambiguous patient statements; competent nurses needed to improve emergency response skills; proficient nurses faced ethical challenges; and experts sought to pass on their knowledge. Stage-appropriate educational programs, such as a Visual Pain and Psychiatric Symptoms Evaluation Sheet, were proposed accordingly.

    DISCUSSION: Further investigations should assess the effectiveness of these educational programs, Japanese-Malaysian cultural differences, and psychiatric liaison nursing.

    MeSH terms: Adult; Clinical Competence; Female; Humans; Interviews as Topic; Japan; Malaysia; Male; Middle Aged; Nurse's Role; Qualitative Research*
  15. Zhu CZ, Samuel OD, Taheri-Garavand A, Elboughdiri N, Paramasivam P, Hussain F, et al.
    Sci Rep, 2025 Feb 15;15(1):5638.
    PMID: 39955378 DOI: 10.1038/s41598-025-88901-9
    Researchers and stakeholders have shown interest in heterogeneous composite biodiesel (HCB) due to its enhanced fuel properties and environmental friendliness (EF). The lack of high viscosity datasets for parent hybrid oils has hindered their commercialisation. Reliable models are lacking to optimise the transesterification parameters for developing HCB, and the scarcity of predictive models has affected climate researchers and environmental experts. In this study, basic fuel properties were analysed, and models were developed models for the yield of HCB and kinematic viscosity (KV) for composite biodiesel/neem castor seed oil methyl ester (NCSOME) using Artificial Neural Network (ANN) and Adaptive Neuro Fuzzy Inference System (ANFIS). Statistical indices such as computed coefficient of determination (R2), root-mean-square-error (RMSE), standard error of prediction (SEP), mean average error (MAE), and average absolute deviation (AAD) were used to evaluate the effectiveness of the techniques. Emission models for NCSOME-diesel blends were also established. The study investigated the impact of optimised fuel types/NCSOME-diesel (10-30 vol%), ZnO nanoparticle dosage (400-800 ppm), engine speed (1100-1700 rpm), and engine load (10-30%) on emission characteristics and environmental friendliness indices (EFI) such as carbon monoxide (CO), Oxides of Nitrogen (NOx), and Unburnt Hydrocarbon (UHC) using Response Surface Methodology (RSM). The ANFIS model demonstrated superior performance in terms of R2, RMSE, SEP, MAE, and AAD compared to the ANN model in predicting yield and KV of HCB. The optimal emission levels for CO (49.26 ppm), NOx (0.5171 ppm), and UHC (2.783) were achieved with a fuel type of 23.4%, nanoparticle dosage of 685.432 ppm, engine speed of 1329.2 rpm, and engine load of 10% to ensure cleaner EFI. The hybrid ANFIS and ANN models can effectively predict and model fuel-related characteristics and improve the HCB process, while the RSM model can be a valuable tool for climate and environmental stakeholders in accurate forecasting and promoting a cleaner environment. The valuable datasets can also provide reliable information for strategic planning in the biodiesel and automotive industries.
  16. Lim JX, Yong YK, Dewi FRP, Chan SY, Lim V
    Drug Deliv Transl Res, 2025 Feb 15.
    PMID: 39955406 DOI: 10.1007/s13346-025-01790-3
    Doxorubicin (DOX), an anthracycline, is widely used in cancer treatment by interfering RNA and DNA synthesis. Its broad antitumour spectrum makes it an effective therapy for a wide array of cancers. However, the prevailing drug-resistant cancer has proven to be a significant drawback to the success of the conventional chemotherapy regime and DOX has been identified as a major hurdle. Furthermore, the clinical application of DOX has been limited by rapid breakdown, increased toxicity, and decreased half-time life, highlighting an urgent need for more innovative delivery methods. Although advancements have been made, achieving a complete cure for cancer remains elusive. The development of nanoparticles offers a promising avenue for the precise delivery of DOX into the tumour microenvironment, aiming to increase the drug concentration at the target site while reducing side effects. Despite the good aspects of this technology, the classical nanoparticles struggle with issues such as premature drug leakage, low bioavailability, and insufficient penetration into tumours due to an inadequate enhanced permeability and retention (EPR) effect. Recent advancements have focused on creating stimuli-responsive nanoparticles and employing various chemosensitisers, including natural compounds and nucleic acids, fortifying the efficacy of DOX against resistant cancers. The efforts to refine nanoparticle targeting precision to improve DOX delivery are reviewed. This includes using receptor-mediated endocytosis systems to maximise the internalisation of drugs. The potential benefits and drawbacks of these novel techniques constitute significant areas of ongoing study, pointing to a promising path forward in addressing the challenges posed by drug-resistant cancers.
  17. Li X, Liu J, He L, Tian M, Xu Y, Peng B
    Protein Pept Lett, 2025 Feb 11.
    PMID: 39950465 DOI: 10.2174/0109298665339026250114070523
    INTRODUCTION: Endogenous microRNAs (miRNAs) are critical regulators of tumor progression, making their role in breast cancer an important area of investigation.

    METHOD: This study examined the regulation of MSMO1 by miR-584-5p in breast cancer cells. Using bioinformatics and Western blotting, we confirmed MSMO1 expression in breast cancer cells and evaluated its effects on cell migration, invasion, and the AKT signaling pathway. In vivo experiments further supported these findings. The interaction between miR-584-5p and MSMO1 was validated through luciferase reporter assays, while functional studies highlighted the impact of miR-584-5p on cancer progression.

    RESULT: Our findings revealed that MSMO1 is upregulated in breast cancer, enhancing cell migration and invasion. Silencing MSMO1 diminished AKT pathway activity, and luciferase assays confirmed MSMO1 as a direct target of miR-584-5p.

    CONCLUSION: Overexpression of miR-584-5p suppressed migration and invasion of breast cancer cells. In summary, miR-584-5p is likely to modulate MSMO1 and subsequently regulate the AKT/ PI3K pathway, presenting a promising therapeutic target for breast cancer treatment.

  18. Noordin MAM, Najm AA, Dyari HRE, Law D, Alwi SSS, Lazim AM, et al.
    Mini Rev Med Chem, 2025 Feb 11.
    PMID: 39950486 DOI: 10.2174/0113895575362479241231054240
    The broad-spectrum action and capacity to target drug-resistant infections make synthetic Antimicrobial Peptides (AMPs) popular therapeutic agents. Indeed, the effective use of these peptides in clinical application relies on a thorough understanding of their Pharmacokinetic (PK) and ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) characteristics. Despite growing research on synthetic AMPs, there is a notable gap in the literature specifically addressing their ADMET profiles. Previous reviews have not extensively covered this area, providing a vital opportunity to study synthetic AMPs' pharmacokinetics and safety, which are crucial for their therapeutic development. This review covered research studies that focused on PK and ADMET of synthetic antimicrobial peptides from several databases, including Google Scholar, SCOPUS, PubMed, and Science Direct, within the years 2020 to 2024, and 12 related research papers have been found. AMPs display a wide range of PK behaviors, including rapid renal clearance, liver-centric distribution, broad distribution with low toxicity, high kidney retention, and gradual absorption with dose-dependent toxicity. Overall, the ADMET profiles of AMPs are crucial in assessing their therapeutic potential, and continuous study is necessary to enhance their practical feasibility. An in-depth investigation of the in vivo ADMET and pharmacokinetic profiles of synthetic AMPs is presented in this review to address the current gap in the research. The findings of this study provide important insights for developing synthetic AMPs as effective antimicrobial drugs.
  19. Li J, Davidson PM, Fong DYT, Li Y, Lok KYW, Wong JYH, et al.
    J Glob Health, 2025 Feb 14;15:04091.
    PMID: 39950570 DOI: 10.7189/jogh.15.04091
    BACKGROUND: Given the limited understanding of individuals' positive gains, this study aimed to identify these gains that could be leveraged by policymakers to enhance future health and societal resilience.

    METHODS: We used a global qualitative approach to survey adults over 18 from 30 countries across six World Health Organization (WHO) regions, who detailed up to three personal positive gains from COVID-19 pandemic via an open-ended question. Inductive thematic analysis was employed to identify main themes, and quantitative methods were used for demographic and regional comparisons based on the percentage of responses for each theme.

    RESULTS: From 35 911 valid responses provided by 13 853 participants, six main themes (one negative theme), 39 subthemes, and 673 codes were identified. Five positive gain themes emerged, ordered by response frequency: 1) improved health awareness and practices; 2) strengthened social bonds and trust; 3) multi-dimensional personal growth; 4) resilience and preparedness building; 5) accelerated digital transformation. The percentage of responses under these themes consistently appeared in the same order across various demographic groups and economic development levels. However, there were variations in the predominant theme across WHO regions and countries, with either Theme 1, Theme 2, or Theme 3 having the highest percentage of responses. Although our study primarily focused on positive gains, unexpectedly, 12% of responses (4304) revealed 'negative gains', leading to an unforeseen theme: 'Distrust and emerging vulnerabilities.' While this deviates from our main topic, we retained it as it provides valuable insights. Notably, these 'negative gains' had a higher percentage of responses in areas like Burundi (94.1%), Rwanda (31.8%), Canada (26.9%), and in the African Region (37.7%) and low-income (43.9%) countries, as well as among non-binary individuals, those with lower education, and those facing employment challenges.

    CONCLUSIONS: Globally, the identified diverse positive gains guide the domains in which health policies and practices can transform these transient benefits into enduring improvements for a healthier, more resilient society. However, variations in thematic responses across demographics, countries, and regions highlights need for tailored health strategies.

    MeSH terms: Adolescent; Adult; Aged; Female; Humans; Health Knowledge, Attitudes, Practice; Male; Middle Aged; Surveys and Questionnaires; Global Health; World Health Organization; Qualitative Research*; Resilience, Psychological; Young Adult; Pandemics
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