Browse publications by year: 2025

  1. Koh WC, Yusoff K, Song AAL, Saad N, Chia SL
    J Med Microbiol, 2025 Feb;74(2).
    PMID: 39950625 DOI: 10.1099/jmm.0.001972
    RNA interference regulates gene expression by selectively silencing target genes through the introduction of small RNA molecules, such as microRNA, small interfering RNA and short hairpin RNA. These molecules offer significant therapeutic potential for diverse human ailments like cancer, viral infections and neurodegenerative disorders. Whilst non-viral vectors like nanoparticles have been extensively explored for delivering these RNAs, viral vectors, with superior specificity and delivery efficiency, remain less studied. This review examines current viral vectors for small RNA delivery, focusing on design strategies and characteristics. It compares the advantages and drawbacks of each vector, aiding readers in selecting the optimal one for small RNA delivery.
    MeSH terms: Animals; Genetic Vectors*; Humans; Viruses/genetics; Gene Transfer Techniques; RNA Interference; MicroRNAs/genetics
  2. Ahmad AA, Lalonde DH
    Int Orthop, 2025 Feb 14.
    PMID: 39951053 DOI: 10.1007/s00264-025-06432-4
    PURPOSE: Wide Awake Local Anesthesia No Tourniquet (WALANT) has gained significant attention since its introduction in 2005. Over 343 publications since 2013 highlight its increasing use in nerve decompression surgeries. WALANT is known for its safety benefits, cost-effectivenes and improved patient experience compared to traditional sedation and tourniquet-based methods. This review examines the advantages of WALANT in nerve decompression surgery, emphasizing its growing adoption and benefits.

    METHODS: A comprehensive review of the WALANT technique is provided, focusing on injection strategies, including buffered lidocaine with epinephrine, proper needle placement and generous volume of tumescent anesthesia. Specific injection protocols and surgical approaches are discussed for various nerve decompression procedures such as carpal tunnel, lacertus, cubital tunnel, radial tunnel and lateral intermuscular septum releases.

    RESULTS: WALANT eliminates sedation-related risks such as nausea, aspiration pneumonia and thromboembolism, making it suitable for high-risk patients (ASA 4). It reduces healthcare costs by decreasing the need for post-anesthesia care units and operating in minor procedure rooms. The technique fosters greater surgeon-patient interaction, reduces hospital stays and avoids preoperative fasting and testing. WALANT has demonstrated positive outcomes across multiple nerve decompression procedures.

    CONCLUSION: WALANT provides a transformative approach in nerve decompression surgery, offering enhanced safety, cost-effectiveness and improved patient-centered care. It significantly contributes to positive surgical outcomes, making it a valuable technique for both patients and healthcare providers.

  3. Tan YH, Yoon DH, Davies AJ, Buske C, Boo YL, Somasundaram N, et al.
    Discov Oncol, 2025 Feb 14;16(1):181.
    PMID: 39951161 DOI: 10.1007/s12672-025-01860-5
    Chimeric antigen receptor T-cell (CAR-T)-mediated therapies have shown promising clinical benefit in patients with refractory or relapsing (R/R) diffuse large B-cell lymphoma (DLBCL). However, CAR-T treatment presents challenges such as lack of drug accessibility, financial barriers, variable physician preference or experience, and risk assessment based on patient-specific characteristics. This article thus aims to provide an overview of the CAR-T landscape for R/R DLBCL in Asia, with a focus on identifying barriers to access, from the perspective of Asian and international lymphoma experts. Presently, existing clinical data indicate that CAR-T therapy is a potentially curative strategy for R/R DLBCL in addition to stem cell transplantation, provided the patient's disease profile and treatment history have been thoroughly considered. However, longer-term follow-up data from large-scale studies are needed to confirm curative potential and define optimal sequencing of CAR-T in the context of novel emerging treatments, such as bi-specific antibodies, in the management of R/R DLBCL. Consequently, further research into CAR-T would benefit from collaboration between institutions. Furthermore, there is a wide disparity in CAR-T accessibility across regions due to complicated logistics and cost, which represent a significant barrier to patients in Asia. Hence, there is a need to increase representation and engagement across different stakeholders such as policymakers, payers, and the industry to arrive at a consensus on patient selection, establish clear guidelines, and develop strategies to lower CAR-T costs. Ultimately, data can support a multi-stakeholder approach when devising strategies to make CAR-T feasible and sustainable for patients.
  4. Chong GY, Kaur S, Talib RA, Loy SL, Tan HY, Mok KHW, et al.
    PLoS One, 2025;20(2):e0313931.
    PMID: 39951411 DOI: 10.1371/journal.pone.0313931
    Chrononutrition, which examines the relationship between circadian rhythms and nutrition, has been associated with glycemic outcomes in adults. However, published data on delayed meal timing, increased meal frequency and frequent breakfast skipping have shown inconsistent glycemic outcomes due to variations in methodologies and populations studied. This review presents the scoping review protocol designed to map the evidence on the association between chrononutrition factors and glycemic outcomes in adults. The methodology framework from Arksey and O'Malley will be adapted for this scoping review. Relevant publications will be searched on databases including PubMed, EBSCO Host, ProQuest Central, MEDLINE & Ovid, Scopus and Web of Science. This review focuses on original articles published from January 2014 to 2024, involving participants aged 18 years and older, published in English, and encompassing experimental and observational studies. A comprehensive keyword search strategy will be developed to identify relevant articles. Two reviewers will independently screen the abstracts and titles to determine the eligibility. Subsequently, the full text of potentially eligible articles will be reviewed by additional independent reviewer for final inclusion, with full text screening being verified by two reviewers, and interrater reliability will be conducted. Data from the included articles will be extracted, collated and charted to summarize the relevant methods, outcomes and key findings. This Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) checklist will be used to guide the development of protocol. This scoping review represents a novel approach to summarize the association between chrononutrition factors and glycemic outcomes among adults. We anticipate the findings of the review will provide stakeholder with crucial evidence-based information for development of effective intervention to manage glycemic outcome in adults. This protocol has been prospectively registered in the Open Science Framework (https://doi.org/10.17605/OSF.IO/PA9BU).
    MeSH terms: Adult; Feeding Behavior/physiology; Humans; Meals/physiology
  5. Hayrapetyan A, Tumasyan A, Adam W, Andrejkovic JW, Bergauer T, Chatterjee S, et al.
    Phys Rev Lett, 2025 Jan 31;134(4):041801.
    PMID: 39951610 DOI: 10.1103/PhysRevLett.134.041801
    A search is presented for an extended Higgs sector with two new particles, X and ϕ, in the process X→ϕϕ→(γγ)(γγ). Novel neural networks classify events with diphotons that are merged and determine the diphoton masses. The search uses LHC proton-proton collision data at sqrt[s]=13  TeV collected with the CMS detector, corresponding to an integrated luminosity of 138  fb^{-1}. No evidence of such resonances is seen. Upper limits are set on the production cross section for m_{X} between 300 and 3000 GeV and m_{ϕ}/m_{X} between 0.5% and 2.5%, representing the most sensitive search in this channel.
  6. Pasamanickam K, Rajadurai J, Choo GH
    Int J Angiol, 2025 Mar;34(1):60-63.
    PMID: 39944147 DOI: 10.1055/s-0040-1715084
    We present the first clinical report and describe the technique of using a modified Heartrail II guiding catheter to successfully puncture and remove a coronary angioplasty balloon that failed to deflate after stent implantation.
  7. Gunasaykaran SY, Chear NJ, Ismail S, Mohammad NA, Murugaiyah V, Ramanathan S
    Naunyn Schmiedebergs Arch Pharmacol, 2025 Feb;398(2):1447-1464.
    PMID: 39325152 DOI: 10.1007/s00210-024-03418-8
    Herbal medicines are widely used as alternative or complementary therapies to treat and prevent chronic diseases. However, these can lead to drug-drug interactions (DDIs) that affect the glucuronidation reaction of UDP glucuronosyltransferases (UGTs), which convert drugs into metabolites. Plant extracts derived from medicinal herbs contain a diverse array of compounds categorized into different functional groups. While numerous studies have examined the inhibition of UGT enzymes by various herbal compounds, it remains unclear which group of compounds exerts the most significant impact on DDIs in the glucuronidation reaction. Recently, alkaloids derived from medicinal herbs, including kratom (Mitragyna speciosa), have gained attention due to their diverse pharmacological properties. This review primarily focuses on the DDIs of plant alkaloids from medicinal herbs, including kratom on the phase II UGT enzymes. Kratom is a new emerging herbal product in Western countries that is often used to self-treat chronic pain, opioid withdrawal, or as a replacement for prescription and non-prescription opioids. Kratom is well-known for its psychoactive alkaloids, which have a variety of psychopharmacological effects. However, the metabolism mechanism of kratom alkaloids, particularly on the phase II pathway, is still poorly understood. Simultaneously using kratom or other herbal products containing alkaloids with prescribed medicines may have an impact on the drug metabolism involving the phase II UGT enzymes. To ensure the safety and efficacy of treatments, gaining a better understanding of the DDIs when using herbal products with conventional medicine is crucial.
    MeSH terms: Animals; Drug Interactions; Humans; Plant Extracts/pharmacology; Plants, Medicinal/chemistry; Herb-Drug Interactions
  8. Koh CMM, Hwang SS, Lau BT, Palombo EA, Ginjom IRH, Ha CHX, et al.
    ACS Infect Dis, 2025 Feb 14;11(2):413-429.
    PMID: 39423324 DOI: 10.1021/acsinfecdis.4c00549
    Pseudomonas aeruginosa is the predominant bacterium found in many chronic biofilm infections. Over the past few decades, biofilm-related infections have posed a significant challenge to medical practice due to the increasing emergence of multidrug resistance. Cis-2-decenoic acid (CDA), a small molecule found in P. aeruginosa, has been shown to disperse biofilms formed by various bacteria and to work in synergy with common antibiotics. Despite that, the binding mechanism between CDA and the predicted cyclases/histidine kinases associated sensory extracellular (CHASE) domain of sensor protein DspS remains unknown in the absence of a crystallized protein structure. Moreover, the therapeutic potential of CDA is limited by its susceptibility to oxidative degradation and isomerization. In this work, we propose a structural model for the DspS CHASE domain. The resulting model displays an overall topology reminiscent of the sensor protein PcrK in Xanthomonas campestris. Through molecular dynamics simulations, a stable potential binding site for CDA was further identified. Virtual screening against the predicted site of DspS CHASE using our developed pipeline discovered two promising compounds, compounds 2 and 9, capable of dislodging 7-day P. aeruginosa biofilms at 50 μM without affecting bacterial growth. These compounds also enhanced the effects of ciprofloxacin against P. aeruginosa, reduced the survival of dispersed cells, and increased the expression of matrix-degrading enzyme genes pelA, pslG, and eddA. This study provides insights into CDA recognition by DspS and represents the first large-scale effort to uncover first-in-class DspS activators. At the same time, this work also underscores the effectiveness of a computational-aided drug discovery process in finding new activators, even without a known protein structure.
    MeSH terms: Binding Sites; Fatty Acids, Monounsaturated; Molecular Dynamics Simulation
  9. Lung SC, Chye ML
    Plant Physiol, 2025 Feb 07;197(2).
    PMID: 39431550 DOI: 10.1093/plphys/kiae565
    While lipids serve as important energy reserves, metabolites, and cellular constituents in all forms of life, these macromolecules also function as unique carriers of information in plant communication given their diverse chemical structures. The signal transduction process involves a sophisticated interplay between messengers, receptors, signal transducers, and downstream effectors. Over the years, an array of plant signaling proteins have been identified for their crucial roles in perceiving lipid signals. However, the mechanistic effects of lipid binding on protein functions remain largely elusive. Recent literature has presented numerous fascinating models that illustrate the significance of protein-lipid interactions in mediating signaling responses. This review focuses on the category of lipophilic signaling proteins that encompass a hydrophobic binding pocket located outside of cellular membranes and provides an update on the lessons learned from two of these structures, namely the acyl-CoA-binding and steroidogenic acute regulatory protein-related lipid transfer domains. It begins with a brief overview of the latest advances in understanding the functions of the two protein families in plant communication. The second part highlights five functional mechanisms of lipid ligands in concert with their target signaling proteins.
    MeSH terms: Acyl Coenzyme A/metabolism; Binding Sites; Plants/metabolism; Protein Binding; Solubility; Signal Transduction*; Lipid Metabolism; Protein Domains
  10. Kow CS, Ramachandram DS, Hasan SS, Thiruchelvam K
    Transfusion, 2025 Feb;65(2):260-266.
    PMID: 39709621 DOI: 10.1111/trf.18110
    INTRODUCTION: Acute myocardial infarction (AMI) poses a significant global health burden, warranting meticulous management strategies, particularly in patients with concurrent anemia. Blood transfusion strategies play a pivotal role in optimizing oxygen delivery while minimizing transfusion-related risks. Two contrasting approaches, liberal and restrictive transfusion strategies, have emerged, yet their comparative effectiveness remains uncertain due to conflicting evidence.

    METHODS: We conducted a systematic review and meta-analysis focusing on randomized controlled trials (RCTs) comparing liberal versus restrictive transfusion strategies in AMI patients with anemia (hemoglobin levels <10 g/dL). Comprehensive searches were performed across electronic databases up to March 31, 2024. Data extraction, risk of bias assessment using Cochrane's RoB 2 tool, and meta-analysis were conducted following methodology.

    RESULTS: Among 327 initially identified studies, four high-quality RCTs met the inclusion criteria. These trials encompassed varied sample sizes and patient demographics. Meta-analysis revealed no significant difference in 30-day all-cause mortality nor recurrent MI between liberal and restrictive transfusion strategies. However, combining these outcomes into a composite measure demonstrated a significant reduction favoring liberal transfusion (pooled odds ratio = 0.82; 95% confidence interval = 0.69-0.99).

    CONCLUSION: While liberal transfusion strategies show promise in reducing adverse outcomes, clinical decision-making should be guided by individual patient factors and preferences. Personalized care remains paramount in determining the most appropriate transfusion approach for AMI patients with anemia. Further research is warranted to elucidate the optimal transfusion strategy in this population.

    MeSH terms: Humans; Randomized Controlled Trials as Topic*
  11. Jain D
    Int J Gynaecol Obstet, 2025 Mar;168(3):1345-1351.
    PMID: 39711117 DOI: 10.1002/ijgo.16119
    In Asia as elsewhere, strict regulations on who is authorized to provide abortion services and to prescribe or dispense medical abortion adversely impact access, especially for marginalized persons and residents in remote areas. The WHO's 2022 Abortion Care Guideline provides an important framework for states to formulate and implement policies to serve sexual and reproductive rights of access to abortion services. The Guideline calls for decriminalization of abortion services to increase authorization to provide abortion services and to promote self-managed medical abortion. This review examines the legal and policy frameworks of Bangladesh, India, Indonesia, Malaysia, and Nepal against the WHO Guideline. Legal and policy reforms successfully introduced in Bangladesh and Nepal to grow the provider base allow healthcare systems to expand safe abortion. This review outlines further challenges where the WHO Guideline on decriminalization and availability of medical abortion is disregarded and advocates a reproductive justice approach promoting egalitarian access to services even among the most marginalized.
    MeSH terms: Asia; Bangladesh; Female; Health Policy/legislation & jurisprudence; Humans; Nepal; Pregnancy; World Health Organization; Reproductive Rights/legislation & jurisprudence
  12. Wu C, Targher G, Byrne CD, Mao Y, Cheung TT, Yilmaz Y, et al.
    Am J Gastroenterol, 2025 Jan 03.
    PMID: 39749919 DOI: 10.14309/ajg.0000000000003288
    INTRODUCTION: The global burden of metabolic diseases is increasing, but estimates of their impact on primary liver cancer are uncertain. We aimed to assess the global burden of primary liver cancer attributable to metabolic risk factors, including high body mass index (BMI) and high fasting plasma glucose (FPG) levels, between 1990 and 2021.

    METHODS: The total number and age-standardized rates of deaths and disability-adjusted life years (DALYs) from primary liver cancer attributable to each metabolic risk factor were extracted from the Global Burden of Disease Study 1990-2021. The metabolic burden trends of liver cancer across regions and countries by sociodemographic index (SDI) and sex were estimated. The annual percentage changes in age-standardized DALYs rate were also calculated.

    RESULTS: Globally, in 2021, primary liver cancer attributable to high BMI and/or high FPG was estimated to have caused 59,970 deaths (95% uncertainty interval [UI] 20,567-104,103) and 1,540,437 DALYs (95% UI 540,922-2,677,135). The age-standardized rates of death and DALYs were 0.70 (95% UI 0.24-1.21) and 17.64 (95% UI 6.19-30.65) per 100,000 person-years. A consistent global rise in liver cancer attributable to metabolic risks was observed from 1990 to 2021, with high BMI identified as the major contributing risk factor. The highest burden of deaths and DALYs of liver cancer consistently occurred in high SDI countries, while the fastest growth trends were observed in low-middle SDI countries. The burdens of high levels of BMI and FPG were higher in men than in women.

    DISCUSSION: Primary liver cancer attributable to high BMI and/or high FPG imposes an increasingly substantial clinical burden on global public health, particularly in high SDI countries. Rapid growth trends are also found in middle SDI countries.

  13. Chen J, Li W, Zhao J, Lee YY, Chen J, Wang Y
    Int J Biol Macromol, 2025 Feb 10.
    PMID: 39938842 DOI: 10.1016/j.ijbiomac.2025.140884
    The chitosan-based bone-targeted delivery system was designed to enhance the therapy efficacy of Cyclolinopeptide J (CLJ), a bioactive peptide derived from flaxseed, for the treatment of osteoporosis. The bone-targeting polymer conjugates (CSD8) were prepared via a crosslinking reaction between carboxylated chitosan (CMCS) and functional peptide (ASP8). The CSD8 was then modified on the surface of CLJ-loaded nanoparticles to form novel nanoparticles (JCA/CSD8). The particle size of JCA/CSD8 was 122.40 ± 1.8 nm and the loading capacity of CLJ was 22.7 %. The results showed that the in vitro bone affinity and in vivo bone targeting efficiency of JCA/CSD8 increased 11.7-fold and 13.6-fold, respectively, achieving systemic targeting. Moreover, in vitro studies revealed that JCA/CSD8 could degrade within lysosomes under acidic conditions, thereby releasing CLJ and Ca2+ for synergistically promoting osteogenesis to realize the local targeting. The JCA/CSD8 group increased the transcription levels of osteogenic-related markers, including OPG, ColI, OCN, OPN, RUNX2, and ALP. Furthermore, in vivo studies demonstrated the impressive capability of JCA/CSD8 to increase bone density and restore trabecular bone architecture in the OVX mice model, which was superior to the positive control group. In conclusion, using chitosan-based bone-targeted nanoparticles presents a highly promising and efficient clinical therapy for addressing osteoporosis.
  14. Gataa IS, Abdullah Z, González Cabrera MV, S R, Verma S, Arora I, et al.
    PMID: 39939251 DOI: 10.1016/j.numecd.2025.103858
    AIMS: This research delved into a comprehensive examination and detailed analysis of the effects of whey protein (WP) supplementation on lipid profile in adults.

    DATA SYNTHESIS: Data used in this research was obtained from diverse clinical trials. Thorough searches were carried out on multiple electronic platforms including PubMed, Embase, Web of Science, Scopus, Google Scholar, and the Cochrane Library from their inception until May 2024. Random effects models were assessed and pooled data were determined as weighted mean differences (WMDs) with a 95 % confidence interval (CI).

    RESULTS: Overall, 20 randomized clinical trials (n = 1638 participants) met our inclusion criteria. The current meta-analysis demonstrates a significant reduction in TG (WMD: -12.21 mg/dL; %95CI: -20.16, -4.26; P = 0.003). Pooled analysis of 19 studies on HDL-c indicated a significant increase (WMD: 2.59 mg/dL; %95CI: 1.11, 4.07; P = 0.001).

    CONCLUSIONS: We found that WP intake can improve TG and HDL-c significantly without significant effects on TC, and LDL-c levels. However, future well-designed with long duration RCTs is required on diverse populations to understand better the effects of these natural compounds and their constituents on lipid profile in adults.

  15. Lee SK, Keng JW, Yon JA, Mai CW, Lim HC, Chow SC, et al.
    Plants (Basel), 2025 Jan 24;14(3).
    PMID: 39942922 DOI: 10.3390/plants14030362
    To study Cassia alata (CA) (Linnaeus) Roxburgh's effectiveness towards atopic dermatitis (AD), CA leaf extracts were prepared using three methanol-based extraction solvent systems. Bioactive constituents were characterized and quantified via high-performance liquid chromatography with diode array detection. Antioxidant properties and antimicrobial activities against Staphylococcus aureus, a major AD exacerbation factor, were assessed. Four polyphenols (two flavonoids, two anthraquinones) beneficial in AD control were detected (rhein > aloe-emodin > astragalin > kaempferol). The 75% v/v MeOH/water extract had the most polyphenols and the best antioxidant profile (total phenolic content, total flavonoid content, 2,2-diphenyl-1-picrylhydrazyl-hydrate radical scavenging activity, ascorbic acid equivalent antioxidant capacity), with excellent S. aureus inhibition (minimum inhibitory concentration = 0.625 mg/mL; minimum bactericidal concentration = 1.25 mg/mL). Hence, it was selected for the in vitro examination of cytotoxicity and wound healing activity towards human epidermal keratinocyte cells using a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h-tetrazolium bromide (MTT) assay and wound scratch assay. The extract showed no cytotoxicity (IC50 > 100 µg/mL) without significant reduction in cell viability up to 200 µg/mL compared to the vehicle control. An amount of 50 μg/mL extract concentration showed the best wound-healing activity (p < 0.05), with a cell migration rate of 5.89 ± 0.80 µm/h over 96 h post-treatment. Such antioxidant, antimicrobial, and wound-healing activities suggest CA and its polyphenols to be promising natural, long-term AD remedies for skin health.
  16. Masadeh M, Harun SN, Mukattash T, Alrabadi N
    Curr Pediatr Rev, 2025 Feb 12.
    PMID: 39945265 DOI: 10.2174/0115733963365501250210062038
    Antibiotic misuse among children continues to be one of the major critical public health issues worldwide, particularly in developing countries such as Jordan. Incorrect antibiotic use is hugely influenced by parents' knowledge and attitudes on the subject at hand. This review, therefore, seeks to establish the current state of parental knowledge and attitudes toward antibiotics in Jordanian children, with particular emphasis on those gaps in awareness and knowledge that underpin the inappropriate use of antibiotics. Despite prescription regulations, many still depend on over-the-counter purchases, usually due to misconceptions about the efficiency of antibiotics in viral infections or a lack of insight into the risks of misuse. The results strongly suggest that focused educational programs are needed to improve parents' knowledge of prudent antibiotic use. It integrated an analysis of the socio-cultural factors affecting parental decisions and recommended improvising the public health strategies in their fight against antibiotic resistance. The study concluded that emphasis must be laid on both regulatory enforcement and public awareness about appropriate antibiotic use in pediatric care to bring about better health outcomes in Jordanian children.
  17. George S, Johdi NA
    Curr Mol Med, 2025 Feb 12.
    PMID: 39945269 DOI: 10.2174/0115665240355043250130110501
    Colorectal cancer poses a major global health issue, profoundly affecting both mortality and morbidity rates across the world. A key obstacle in understanding the pathogenesis of colorectal cancer lies in its high inter-patient and spatial heterogeneity, making standard treatments ineffective. Commonly, the study on colorectal cancer relies on bulk RNA sequencing, offering an average gene expression profile for a heterogenous cell population. However, this approach obscures the heterogeneous characteristics of the cancer cells and hinders a comprehensive understanding of the complex interplay among different cell populations. Recently, the advent of single-cell RNA sequencing has been revolutionary, enabling researchers to analyze individual immune cells and overcome the limitations of bulk RNA sequencing. Through single-cell RNA sequencing, researchers have gained insights into the dynamic nature of the immune response to cancer and potential targets for immunotherapy. In this review, we discuss the technical aspects of single-cell RNA sequencing, the application of single-cell RNA sequencing in cancer immunology, and the potential of single-cell RNA in clinical settings. We believe that harnessing singlecell RNA sequencing in cancer research holds immense potential to drive the development of personalized immunotherapies, aiming to improve patient outcomes in colorectal cancer.
  18. Cahyaningtyas N, Mahmudiono T, Chen HL, Pui L, Thammakulkrajang R, Wangi MP
    Ital J Food Saf, 2025 Feb 13.
    PMID: 39945432 DOI: 10.4081/ijfs.2025.11136
    Thai tea is one of the beverage products that are often ordered by Generation Z through online applications. With the rise of Thai tea drinks in the market, it is suspected that some individuals cheat by adding cyclamate to Thai tea drinks when concocting to get a good taste and sweetness at a low price. This study aimed to examine the correlation between the level of knowledge and the attitudes of traders regarding the use of cyclamate food additives in Thai tea processed drinks, which are sold through online application-based food delivery services in the Surabaya and Sidoarjo regions. This research was an observational analysis and had a cross-sectional design. The study sample of 46 Thai tea beverage products was taken by simple random sampling. Research shows that there is no link between knowledge (p=0.654), attitudes (p=0.950), and behavior (p=0.849) with the use of cyclamate in Thai tea beverage products. The conclusion of this study is the absence of any connections between knowledge, attitude, behavior, and the use of cyclamates. The results classify Thai tea products, both qualitatively and quantitatively, as safe. Based on questionnaires, respondents had good knowledge, but they also had negative attitudes and behaviors.
  19. Sam'an M, Farikhin, Munsarif M
    PMID: 39945511 DOI: 10.1080/10255842.2025.2460178
    Diabetes is a chronic condition that affects blood sugar levels and vital organs in the body. Early detection is crucial given the increasing global prevalence of diabetes and the grave risk of complications if not properly managed. Thus, a good prediction system is necessary. Although the Decision Tree (DT) is commonly used for classification, it is less effective with large datasets. We propose hyperparameter optimization of the DT using the Grey Wolf Optimization (GWO), which has exploration and both exploitation capabilities. However, the limited search space of GWO may hinder practical exploration and exploitation, leading to premature optimization. To address this, we propose a modified GWO (MGWO) by adding the Levy distribution function to enhance the movements of alpha, beta, and delta wolves. We also provide GA (Genetic Algorithm) as a comparative algorithm. The fitness value of MGWO is 0.8498, surpassing GWO (0.8373) and GA (0.8492). Evaluation results indicate that MGWO and GA yield similar and superior accuracy compared to GWO. The proposed method outperforms existing ones. Further research is needed to evaluate the impact of varying the number of wolves on optimization performance and classification accuracy.
  20. Sun F, Zhang Y, Tan CP, Gu Y, Liu Y, Xu YJ
    Crit Rev Anal Chem, 2025 Feb 13.
    PMID: 39945579 DOI: 10.1080/10408347.2025.2463430
    Microbiomes significantly impact food flavor, food quality and human health. The development of omics technologies has revolutionized our understanding of the microbiome, the generated complex datasets, as well as their processing and interpretation need to be taken seriously. Currently, chemometrics has shown huge potential in omics data analysis, which is crucial to reveal the functional attributes and mechanisms of microbiomes in food nutrition and safety. However, various chemometric tools have their own characteristics, selecting appropriate technologies and performing multiomics data fusion analysis to improve the precision and reliability of food microbial investigations is still a huge challenge. In this review, we summarized the omics technologies used in food microbiome studies, overviewed the principle and applicability of chemometrics in omics, and discussed the challenges and prospects of chemometrics. The urgent need for chemometrics is to integrate deep learning (DL) and artificial intelligence algorithms to enhance its analytical capabilities and prediction accuracy. We hope this review will provide valuable insights of the integration of multiomics and bioinformatics combined with various chemometric techniques in data analysis for food microbial investigation. In the future, chemometrics combined with modern technologies for multiomics data analysis will further deepen our understanding of food microbiology and improve food safety.
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