Novel Nanostructured Lipid Carriers Co-Loaded with Mesalamine and Curcumin: Formulation, Optimization and In Vitro Evaluation

Awasthi A 1 , Kumar B 1 , Gulati M 1 , Vishwas S 1 , Corrie L 1 , Kaur J 1 Show all authors , Khursheed R 1 , Muhammed RA 2 , Kala D 2 , Porwal O 2 , Babu MR 1 , Chaitanya MVNL 1 , Kumar A 1 , Pandey NK 1 , Dureja H 3 , Chellappan DK 4 , Jha NK 5 , Gupta G 6 , Prasher P 7 , Kumar D 8 , Dua K 9 , Singh SK 10

Affiliations 

  • 1 School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, 144411, India
  • 2 Department of Pharmacognosy, Faculty of Pharmacy, Tishk International University-44001, Erbil, Kurdistan Region, Iraq
  • 3 Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana, 124001, India
  • 4 Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil, 57000, Kuala Lumpur, Malaysia
  • 5 Department of Biotechnology, School of Engineering & Technology (SET), Sharda University, Plot No.32-34 Knowledge Park III, Greater Noida, Uttar Pradesh, 201310, India
  • 6 School of Pharmacy, Suresh Gyan Vihar University, Mahal Road, Jagatpura, Jaipur, India
  • 7 Department of Chemistry, University of Petroleum & Energy Studies, Energy Acres, Dehradun, 248007, India
  • 8 Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Shoolini University, Solan, 173229, India
  • 9 Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, NSW, 2007, Australia
  • 10 School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, 144411, India. singhsachin23@gmail.com
Pharm Res, 2022 Nov;39(11):2817-2829.
PMID: 36195824 DOI: 10.1007/s11095-022-03401-z

Abstract

PURPOSE: The aim of current study is to formulate, optimize and characterize the developed formulation of Mesalamine-Curcumin Nanostructured Lipid Carriers (Mes-Cur NLCs).

METHODS: It was formulated using high pressure homogenization followed by probe sonication and formulation variables were optimized using Central Composite Design. The particle size (PS), zeta potential (ZP), entrapment efficiency (EE), drug release, cytotoxicity on NIH 3T3 fibroblasts cells and HaCaT keratinocytes cells and efficacy on RAW264.7 cells for optimized formulation was determined.

RESULTS: The PS, ZP and EE were found to be 85.26 nm, -23.7 ± 7.45 mV, 99.2 ± 2.62 % (Mes) and 84 ± 1.51 % (Cur), respectively. The good correlation between predicted and obtained value indicated suitability and reproducibility of experimental design. NLCs showed spherical shape as confirmed by TEM. In vitro drug release profile of prepared formulation showed that Mes exhibited 100 % release at 48 h, whereas Cur exhibited 82.23 ± 2.97% release at 120 h. Both the drugs exhibited sustained release upon incorporation into the NLCs. The absence of any significant cell death during MTT assay performed on NIH 3T3 fibroblasts cells and HaCaT keratinocytes cells indicated that NLCs' were safe for use. Furthermore, significant reduction in nitric oxide level during anti-inflammatory evaluation of formulation on RAW264.7 cells showed excellent potential for the formulation to treat inflammation. The formulation was found stable as no significant difference between the PS, ZP and EE of the fresh and aged NLCs was observed.

CONCLUSION: The outcomes of study deciphered successful formulation of Mes-Cur NLCs.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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