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Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocomposite

Al-Qubaisi MS 1 , Al-Abboodi AS 2 , Alhassan FH 3 , Hussein-Al-Ali S 4 , Flaifel MH 5 , Eid EEM 6 Show all authors , Alshwyeh HA 7 , Hussein MZ 8 , Alnasser SM 9 , Saeed MI 10 , Rasedee A 1 , Ibrahim WN 11

Affiliations 

  • 1 Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • 2 Basic Science Branch, Faculty of Dentistry, University of Al-Qadisiyah, Al-Diwaniyah, Iraq
  • 3 Department of Applied Chemistry and Technology, College of Science and Arts, Alkamel University of Jeddah, Jeddah 21589, Saudi Arabia
  • 4 Faculty of Pharmacy, Isra University, Amman 11622, Jordan
  • 5 Department of Physics, College of Science, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia
  • 6 Department of Pharmaceutical Chemistry and Pharmacognosy, Unaizah College of Pharmacy, Qassim University, Saudi Arabia
  • 7 Basic & Applied Scientific Research Center, College of Science, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia
  • 8 Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • 9 Department of Pharmacology and Toxicology, Unaizah College of Pharmacy, Qassim University, Saudi Arabia
  • 10 Faculty of Medical Laboratory Sciences, National Ribat University, Khartoum 11111, Sudan
  • 11 Department of Biomedical Science, College of Health Sciences, QU Health, Qatar University, Doha, Qatar
Saudi Pharm J, 2022 Apr;30(4):347-358.
PMID: 35527823 DOI: 10.1016/j.jsps.2022.02.002

Abstract

In this study, we formulated Thymoquinone-loaded nanocomposites (TQ-NCs) using high-pressure homogenizer without sodium tripolyphosphate. The TQ-NCs were characterized and their anti-inflammatory determined by the response of the LPS-stimulated macrophage RAW 264.7 cells in the production of nitric oxide, prostaglandin E2, tumor necrosis factor-α, interleukin-6, and interleukin-1β. The physicochemical properties of TQ-NC were determined using different machines. TQ was fully incorporated in the highly thermal stable nanoparticles. The nanoparticles showed rapid release of TQ in the acidic medium of the gastric juice. In medium of pH 6.8, TQ-NC exhibited sustained release of TQ over a period of 100 h. The results suggest that TQ-NC nanoparticles have potential application as parenterally administered therapeutic compound. TQ-NC effectively reduce production of inflammatory cytokines by the LPS-stimulated RAW 264.7 cells, indicating that they have anti-inflammatory properties. In conclusion, TQ-NC nanoparticles have the characteristics of efficient carrier for TQ and an effective anti-inflammatory therapeutic compound.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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