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Novel Complex of Zinc (II) Dichloroethylenediamine: Synthesis, Characterization, In-silico, and In-vitro Evaluation against Cervical Cancer Cells

Raya I 1 , Kartina D 2 , Wijaya RI 1 , Irfandi R 3 , Abdalrazaq EA 4 , Prihantono P 5 Show all authors , Santi S 6 , Pratiwi E 1 , Khaerunnisa AB 1 , Luthfiana D 7 , Musa B 1 , Natsir H 1 , Maming M 1 , Zainuddin ZD 8 , Ramlawati R 9 , Fudholi A 10 , Usman AN 11 , Supratman U 12 , Mazaya M 13 , Sufiandi S 14

Affiliations 

  • 1 Department of Chemistry, Faculty of Mathematics and Natural Science, Hasanuddin University, Makassar 90245, Indonesia
  • 2 Department of Chemistry, Faculty of Mathematics, and Natural Science, Universitas Pakuan Bogor, 16144 Indonesia
  • 3 Department of Biology Education, Faculty of Teacher Training and Education, Universitas Puangrimaggalatung, Sengkang 90915, Indonesia
  • 4 Chemistry department, Faculty of Science, Al Hussein Bin Talal University, Ma'an- Jordan
  • 5 Department of Surgery, Faculty of Medical, Hasanuddin University, Makassar, 90245, Indonesia
  • 6 Medical Laboratory Technology, Faculty of Health Technology, Megarezky University, Makassar 90234, Indonesia
  • 7 Bioinformatics Research Center, Indonesian Institute of Bioinformatics (INBIO), Malang, Indonesia
  • 8 Department of Biology, Faculty of Mathematics and Natural Science, Hasanuddin University, Makassar 90245, Indonesia
  • 9 Department of Natural Science Education, Faculty of Mathematics and Natural Science, Universitas Negeri Makassar, Makassar, Indonesia
  • 10 Solar Energy Research Institute, Universiti Kebangsaan Malaysia, UKM Bangi, Selangor 43600, Malaysia
  • 11 Midwifery Study Program, Hasanuddin University, Makassar, 90245, Indonesia
  • 12 Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia
  • 13 esearch Center for Computing, Research Organization for Electronics and Informatics, National Research and Innovation Agency (BRIN), Cibinong Science Center, Jl. Raya Jakarta-Bogor KM 46, Cibinong 16911, West Java, Indonesia
  • 14 Directorate of Laboratory Management, Research Facilities, and Science and Technology Park, Deputy for Research and Innovation Infrastructure - The National Research and Innovation Agency of The Republic of Indonesia
Asian Pac J Cancer Prev, 2023 Dec 01;24(12):4155-4165.
PMID: 38156851 DOI: 10.31557/APJCP.2023.24.12.4155

Abstract

OBJECTIVE: Cervical cancer is a malignancy originating from the cervix and often caused by oncogenic Human Papilloma Virus (HPV), specifically subtypes 16 and 18. Anticancer drugs are chemotherapeutic compounds used for cancer treatment. Therefore, this research aims to synthesize and characterize Zinc (II) dichloroethylenediamine (Zn(en)Cl2) complex, as well as determine its antiproliferative activity against HeLa cells. The Zn(en)Cl2 complex was successfully synthesized, and the antiproliferative activity was tested.

METHODS: The synthesis involved reacting ethylenediamine and KCl with Zn metal. The complex formed was characterized using a conductometer, UV-Vis spectroscopy, FT-IR spectroscopy, and XRD, while the activity was measured against HeLa cells.

RESULT: The synthesis yielded a 56.12% conversion with a melting point of 198-200 oC and a conductivity value of 2.02 mS/cm. The Zn(en)Cl2 complex showed potential activity against HeLa cells with an IC50 value of 898.35 µg/mL, which was evidenced by changes in the morphological structure of HeLa cells. Its interaction with DNA targets was investigated by employing molecular docking.

CONCLUSION: The observed data indicated that the Zn(en)Cl2 complex bound to DNA at the nitrogenous base Guanine (DG) by coordinate covalent bonds. Interestingly, DG maintained interaction with the complex until the end of the docking simulation. Additionally, molecular dynamics (MD) simulation was conducted, and the results showed that Zn(en)Cl2 remained bound to the DNA binding pocket all through the process.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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