Anti-cancer Activity of Biogenic Nat-ZnO Nanoparticles Synthesized Using Nyctanthes arbor-tristis (Nat) Flower Extract

Chabattula SC 1 , Gupta PK 2 , Govarthanan K 3 , Varadaraj S 1 , Rayala SK 4 , Chakraborty D 5 Show all authors , Verma RS 6

Affiliations 

  • 1 Department of Biotechnology, Indian Institute of Technology Madras, Chennai, Tamilnadu, 600036, India
  • 2 Department of Life Sciences, Sharda School of Basic Sciences and Research, Sharda University, Greater Noida, Uttar Pradesh, 201310, India
  • 3 Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Science and Regenerative Medicine, Bengaluru, Karnataka, 560065, India
  • 4 Department of Biotechnology, Indian Institute of Technology Madras, Chennai, Tamilnadu, 600036, India. rayala@iitm.ac.in
  • 5 Department of Chemistry, Indian Institute of Technology Madras, Chennai, Tamilnadu, 600036, India. dchakraborty@iitm.ac.in
  • 6 Department of Biotechnology, Indian Institute of Technology Madras, Chennai, Tamilnadu, 600036, India. vermars@faculty.iitm.ac.in
Appl Biochem Biotechnol, 2024 Jan;196(1):382-399.
PMID: 37133677 DOI: 10.1007/s12010-023-04555-1

Abstract

Inorganic nanoparticles (NPs) have played an important role as nano-drug delivery systems during cancer therapy in recent years. These NPs can carry cancer therapeutic agents. Due to this, they are considered a promising ancillary to traditional cancer therapies. Among inorganic NPs, Zinc Oxide (ZnO) NPs have been extensively utilized in cellular imaging, gene/drug delivery, anti-microbial, and anti-cancerous applications. In this study, a rapid and cost-effective method was used to synthesize Nat-ZnO NPs using the floral extract of the Nyctanthes arbor-tristis (Nat) plant. Nat-ZnO NPs were physicochemically characterized and tested further on in vitro cancer models. The average hydrodynamic diameter (Zaverage) and the net surface charge of Nat-ZnO NPs were 372.5 ± 70.38 d.nm and -7.03 ± 0.55 mV, respectively. Nat-ZnO NPs exhibited a crystalline nature. HR-TEM analysis showed the triangular shape of NPs. Furthermore, Nat-ZnO NPs were also found to be biocompatible and hemocompatible when tested on mouse fibroblast cells and RBCs. Later, the anti-cancer activity of Nat-ZnO NPs was tested on lung and cervical cancer cells. These NPs displayed potent anti-cancer activity and induced programmed cell death in cancer cells.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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