Classroom dust microbiome-metabolome signatures and ocular health in students: Cross-sectional associations with putative pollutant-transformation and microbial functional profiles

Fu X 1 , He H 2 , Li C 2 , Huang Q 2 , Bu Y 2 , Zhang D 2 Show all authors , Hashim JH 3 , Hashim Z 4 , Wispriyono B 5 , Sary L 6 , Pratama S 7 , Wieslander G 8 , Norback D 8 , Sun Y 9

Affiliations 

  • 1 Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, School of Public Health, Guangdong Pharmaceutical University, Guangzhou, 510006, PR China; State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Laboratory for the Development Biology and Environmental Adaptation of Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, 510642, PR China. Electronic address: fuxi@gdpu.edu.cn
  • 2 Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, School of Public Health, Guangdong Pharmaceutical University, Guangzhou, 510006, PR China
  • 3 Department of Environmental Health and Occupational Safety, Faculty of Health Sciences, Universiti Selangor, Shah Alam, Selangor, Malaysia; Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, Selangor, Malaysia
  • 4 Department of Environmental and Occupational Health, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
  • 5 Center for Industrial and Environmental Health, Faculty of Public Health, University of Indonesia, Depok, Indonesia
  • 6 Faculty of Medicine, Universitas Malahayati, Bandar Lampung, Indonesia
  • 7 Faculty of Military Medicine, Indonesia Defense University, Bogor, Indonesia
  • 8 Department of Medical Science, Occupational and Environmental Medicine, University Hospital, Uppsala University, Uppsala, Sweden
  • 9 State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Laboratory for the Development Biology and Environmental Adaptation of Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, 510642, PR China. Electronic address: sunyu@scau.edu.cn
Environ Pollut, 2026 Dec 01;410:129217.
PMID: 42778012 DOI: 10.1016/j.envpol.2026.129217

Abstract

Ocular irritation and tear-film instability are common among school-aged children, yet the microbial and chemical features of classroom dust associated with these outcomes remain poorly characterized. We conducted a cross-sectional multi-omics study in 32 classrooms from eight Indonesian junior high schools, integrating shotgun metagenomics and untargeted LC-MS metabolomics of settled dust with ocular symptoms and tear film break-up time (TFBUT) from 380 students. Classrooms with a low prevalence of ocular irritation were enriched in Actinobacteriota such as Allosaccharopolyspora coralli, Saccharopolyspora dendranthemae, and Brevibacterium epidermidis (LDA > 2, P  2, P 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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