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In depth analysis of the Sox4 gene locus that consists of sense and natural antisense transcripts

Ling KH 1 , Brautigan PJ 2 , Moore S 2 , Fraser R 2 , Leong MP 3 , Leong JW 3 Show all authors , Zainal Abidin S 3 , Lee HC 3 , Cheah PS 4 , Raison JM 5 , Babic M 2 , Lee YK 2 , Daish T 5 , Mattiske DM 6 , Mann JR 7 , Adelson DL 5 , Thomas PQ 5 , Hahn CN 2 , Scott HS 8

Affiliations 

  • 1 Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia; School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA 5005, Australia; NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia
  • 2 Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia
  • 3 NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia
  • 4 NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia; School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia; Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor DE, Malaysia
  • 5 School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia
  • 6 Theme of Laboratory and Community Genetics, Murdoch Childrens Research Institute, Royal Children׳s Hospital, Flemington Road, Parkville, VIC 3052, Australia
  • 7 Biomedicine Discovery Institute, Monash University, VIC 3800, Australia
  • 8 Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia; School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA 5005, Australia
Data Brief, 2016 Jun;7:282-90.
PMID: 26958646 DOI: 10.1016/j.dib.2016.01.045

Abstract

SRY (Sex Determining Region Y)-Box 4 or Sox4 is an important regulator of the pan-neuronal gene expression during post-mitotic cell differentiation within the mammalian brain. Sox4 gene locus has been previously characterized with multiple sense and overlapping natural antisense transcripts [1], [2]. Here we provide accompanying data on various analyses performed and described in Ling et al. [2]. The data include a detail description of various features found at Sox4 gene locus, additional experimental data derived from RNA-Fluorescence in situ Hybridization (RNA-FISH), Western blotting, strand-specific reverse-transcription quantitative polymerase chain reaction (RT-qPCR), gain-of-function and in situ hybridization (ISH) experiments. All the additional data provided here support the existence of an endogenous small interfering- or PIWI interacting-like small RNA known as Sox4_sir3, which origin was found within the overlapping region consisting of a sense and a natural antisense transcript known as Sox4ot1.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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