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dsRNA silencing of an R2R3-MYB transcription factor affects flower cell shape in a Dendrobium hybrid

Lau SE 1 , Schwarzacher T 2 , Othman RY 3 , Harikrishna JA 4

Affiliations 

  • 1 Centre for Research in Biotechnology for Agriculture, University of Malaya, 50603, Kuala Lumpur, Malaysia. suee@um.edu.my
  • 2 Department of Biology, University of Leicester, University Road, Leicester, LE1 7RH, United Kingdom. ts32@leicester.ac.uk
  • 3 Centre for Research in Biotechnology for Agriculture, University of Malaya, 50603, Kuala Lumpur, Malaysia. yasmin@um.edu.my
  • 4 Centre for Research in Biotechnology for Agriculture, University of Malaya, 50603, Kuala Lumpur, Malaysia. jennihari@um.edu.my
BMC Plant Biol, 2015;15:194.
PMID: 26260631 DOI: 10.1186/s12870-015-0577-3

Abstract

The R2R3-MYB genes regulate pigmentation and morphogenesis of flowers, including flower and cell shape, and therefore have importance in the development of new varieties of orchids. However, new variety development is limited by the long breeding time required in orchids. In this study, we identified a cDNA, DhMYB1, that is expressed during flower development in a hybrid orchid, Dendrobium hybrida (Dendrobium bobby messina X Dendrobium chao phraya) then used the direct application of dsRNA to observe the effect of gene silencing on flower phenotype and floral epidermal cell shape.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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