Proteins recovery from wet microalgae using liquid biphasic flotation (LBF)

Phong WN 1 , Show PL 2 , Teh WH 3 , Teh TX 3 , Lim HMY 3 , Nazri NSB 3 Show all authors , Tan CH 3 , Chang JS 4 , Ling TC 1

Affiliations 

  • 1 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 2 Bioseparation Research Group, Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia; Manufacturing and Industrial Processes Division, Faculty of Engineering, Centre for Food and Bioproduct Processing, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia. Electronic address: pauloke.show@nottingham.edu.my
  • 3 Bioseparation Research Group, Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia
  • 4 Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan
Bioresour Technol, 2017 Nov;244(Pt 2):1329-1336.
PMID: 28602664 DOI: 10.1016/j.biortech.2017.05.165

Abstract

In this work, the extraction of microalgal protein from wet Chlorella sorokiniana species using alcohol/salt liquid biphasic flotation (LBF) with the aid of ultrasonication for cell rupturing was proposed. The effect of varying crude feedstock concentration, flotation time, salt type, salt concentration, alcohol type, alcohol concentration, initial volumes of salt and alcohol were investigated. After the optimization process, the highest proportion of protein recovered in the top phase was achieved with 250g/L ammonium sulphate, 60% (v/v) 2-propanol, 1.0VR,initial, 20g/L crude biomass load, 4mm3/min air flowrate and 10min of flotation time. The recycling of phase components was introduced to minimize the use of alcohol and salt in the corresponding LBF. It was demonstrated that top phase (alcohol) recycling can achieve increasing performance for three consecutive recycling runs. Under optimized process conditions, the proportion of protein recovered in the top phase was 88.86% for the third recycle run.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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