Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03

Tan CH 1 , Show PL 2 , Ling TC 3 , Nagarajan D 4 , Lee DJ 5 , Chen WH 6 Show all authors , Chang JS 7

Affiliations 

  • 1 Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia
  • 2 Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia. Electronic address: PauLoke.Show@nottingham.edu.my
  • 3 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 4 Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan
  • 5 Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan
  • 6 Department of Aeronautics and Astronautics, National Cheng Kung University, Tainan, Taiwan; Research Center for Circular Economy, National Cheng Kung University, Tainan 701, Taiwan
  • 7 Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Research Center for Circular Economy, National Cheng Kung University, Tainan 701, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan; College of Engineering, Tunghai University, Taichung 407, Taiwan
Bioresour Technol, 2019 Aug;285:121331.
PMID: 30999192 DOI: 10.1016/j.biortech.2019.121331

Abstract

Third generation biofuels, also known as microalgal biofuels, are promising alternatives to fossil fuels. One attractive option is microalgal biodiesel as a replacement for diesel fuel. Chlamydomonas sp. Tai-03 was previously optimized for maximal lipid production for biodiesel generation, achieving biomass growth and productivity of 3.48 ± 0.04 g/L and 0.43 ± 0.01 g/L/d, with lipid content and productivity of 28.6 ± 1.41% and 124.1 ± 7.57 mg/L/d. In this study, further optimization using 5% CO2 concentration and semi-batch operation with 25% medium replacement ratio, enhanced the biomass growth and productivity to 4.15 ± 0.12 g/L and 1.23 ± 0.02 g/L/d, with lipid content and productivity of 19.4 ± 2.0% and 239.6 ± 24.8 mg/L/d. The major fatty acid methyl esters (FAMEs) were palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:2). These short-chain FAMEs combined with high growth make Chlamydomonas sp. Tai-03 a suitable candidate for biodiesel synthesis.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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