Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane

Ng IS 1 , Tang MSY 2 , Show PL 3 , Chiou ZM 4 , Tsai JC 4 , Chang YK 5

Affiliations 

  • 1 Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan
  • 2 Institute of Biological Study, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Low Dimensional Material Research Center (LDMRC), Department of Physics, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 3 Department of Chemical Engineering and Environmental Engineering, University of Nottingham Malaysia Campus, Selangor Darul Ehsan, Malaysia
  • 4 Department of Chemical Engineering/Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City 24301, Taiwan
  • 5 Department of Chemical Engineering/Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City 24301, Taiwan. Electronic address: ykchang@mail.mcut.edu.tw
Int J Biol Macromol, 2019 Jul 01;132:615-628.
PMID: 30940588 DOI: 10.1016/j.ijbiomac.2019.03.235

Abstract

In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1-1.0 M), chitosan coupling pH (6-8), chitosan coupling concentration (0.1-3.0%), algal solution pH (6-8), algal mass concentration (0.1-1.0% dw/v), and membrane flux (4.08 × 10-2-2.04 × 10-1 mL/min·cm2) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP) > allophycocyanin (APC) > CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step.

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