Sains Malaysiana, 2015;44:1693-1699.

Abstract

This paper presents a low-cost method of constructing the compact UV illuminator, which is considered as an important
component of a gel documentation system. The procedure involves using a smallest-possible UV lamp and a motor which
moves the UV lamp in the UV illuminator instead of conventional 4 UV lamps. A comparative analysis of images produced
by using the commercial gel documentation system and our prototype was carried out. These comparisons were done
in real DNA gel as well as a reference plate made of quantum dot. The plate was composed of the chambers filled with
various densities of the quantum dot instead of the Agarose gel containing the ETBR in order to increase the accuracy of
comparison and the convenience of experiments. Despite the use of only 1 UV lamp, the proposed system demonstrated
a similar imaging performance compared with the conventional gel documentation system equipped with 4 UV lamps,
resulting in the great reduction of the system cost.