Phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts

Salahuddin MAH 1 , Ismail A 2 , Kassim NK 3 , Hamid M 4 , Ali MSM 5

Affiliations 

  • 1 Research Centre of Excellence, Nutrition and Non-Communicable Diseases, Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Malaysian Agricultural Research and Development Institute, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia. Electronic address: mirfat@mardi.gov.my
  • 2 Research Centre of Excellence, Nutrition and Non-Communicable Diseases, Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: aminis@upm.edu.my
  • 3 Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: kartinee@upm.edu.my
  • 4 Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: muhajir@upm.edu.my
  • 5 Malaysian Agricultural Research and Development Institute, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia. Electronic address: mshukri@mardi.gov.my
Food Chem, 2020 Nov 30;331:127240.
PMID: 32585546 DOI: 10.1016/j.foodchem.2020.127240

Abstract

The present study focused on the phytochemical profiling along with evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of various crudes and fractions obtained from Lepisanthes fruticosa (Roxb) Leenh fruit. Ethanolic seed crude extract exhibited the strongest radical scavenging, β-carotene bleaching activity, α-glucosidase inhibition and the highest total phenolic content (TPC). Column chromatography afforded various fractions with fraction M4 being the most potent due to the strongest radical scavenging, β-carotene bleaching, α-glucosidase inhibition and greatest amount of TPC. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of ethanolic seed crude extract and fraction M4 showed the presence of various phytochemicals with antioxidant and antidiabetic properties, which include mostly flavonoids and tannins. The results may suggest that the ethanolic crude seed extract and its fraction could be an excellent source of bioactive phytochemicals with antioxidant and antidiabetic potential.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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