Objectives: To assess the prevalence of handedness and its impact on the perceived difficulty in clinical training amongst undergraduate dental students in Malaysia. Materials and Methods: A cross-sectional survey using a self-administered questionnaire was conducted in 5 dental schools around the Klang Valley. Samples consisted of year 4 and year 5 clinical undergraduate dental students. Information on the socio-demographic data, the determination of handedness and assesment of perceived difficulty in carrying out certain clinical procedures were collected. Results: A total sample of 347 participants of which 76.9% were females was obtained. The distribution of year 4 and year 5 students in the sample were equal. Eighty five percent of the samples were right-handed, 9.5% were ambidextrous and 5.5% were left-handed. Statistically significant difference was found between handedness groups for the combined clinical procedures variable: F (2.100), P