Affiliations 

  • 1 Integrative Medicine Clusters, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, 13200, Kepala Batas, Penang, Malaysia
  • 2 Department of Chemistry, Faculty of Science and Centre for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, 81310, UTM, Johor Bahru, Johor, Malaysia
  • 3 Fundamental & Applied Sciences Department, Universiti Teknologi PETRONAS, 32601, Seri Iskandar, Perak Darul Ridzuan, Malaysia
  • 4 Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor Darul Ehsan, Malaysia
  • 5 School of Chemical Sciences, Universiti Sains Malaysia, 11800, USM Pulau Pinang, Malaysia
  • 6 Integrative Medicine Clusters, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, 13200, Kepala Batas, Penang, Malaysia. Electronic address: noorfatimah@usm.my
  • 7 Department of Chemistry, University of British Columbia, Vancouver, V6T 1Z1, Canada. Electronic address: chen@chem.ubc.ca
Talanta, 2022 Jan 01;236:122833.
PMID: 34635223 DOI: 10.1016/j.talanta.2021.122833

Abstract

A dynamic pH junction was used in capillary electrophoresis (CE-DAD) to on-line preconcentrate, separate, and determine trace amounts of sulfonamide antibiotics (SAs) in milk and yoghurt samples in this study. A sample matrix with 0.15% acetic acid and 10% methanol (MeOH) at a pH of 4.0, and a background electrolyte (BGE) that contained 35 mM sodium citrate with 10% MeOH at a pH of 8.5, and an acidic barrage of 0.4% acetic acid with 10% MeOH at a pH of 2.5 were utilised to achieve a stacking effect for SAs through a dynamic pH junction. Under optimised conditions, the proposed preconcentration method showed good linearity (30-500 ng/mL, r2 ≥ 0.9940), low limits of detection (LODs) of 4.1-6.3 ng/mL, and acceptable analytes recovery (81.2-106.9%) with relative standard deviations (RSDs) within 5.3-13.7 (n = 9). The limits of quantification (LOQs) were below the maximum residue limit approved by the European Union (EU) in this type of matrices. Sensitivity enhancement factors of up to 129 were reached with the optimised dynamic pH junction using CE with a diode array detector (DAD). The method was used to determine SAs in fresh milk, low-fat milk, full-cream milk, and yoghurt samples.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Similar publications