MyMedR

Displaying publications 1 - 20 of 59 in total

Abstract:

Sort:

Fulltext Intestinal nematode infections and efficacy study of oxantel-pyrantel pamoate among plantation workers

Zahedi M, Oothuman P, Sabapathy NN, Abu Bakar N

Med J Malaysia, 1980 Sep;35(1):31-7.
PMID: 7253995
Imaging of an atypical pericardial cyst

Abu Bakar N, Abdul Aziz YF, Singh Sandhu R, Fadzli F, Yaakub NA, Krishnasamy S, et al.

Heart Lung Circ, 2013 Apr;22(4):305-8.
PMID: 22921797 DOI: 10.1016/j.hlc.2012.07.016

Pericardial cysts are uncommon mediastinal lesions that are usually congenital in origin. Patients are usually asymptomatic and the cysts are generally discovered on routine radiography for unrelated purposes. This case illustrates a symptomatic presentation of a haemorrhagic pericardial cyst in which radiographic, echocardiographic and multislice (MRI and CT) imaging were performed. The imaging findings were not typical of pericardial cysts leading to a diagnostic quandary despite the varied imaging techniques. In conclusion, it is important to appreciate that the imaging findings of pericardial cysts can be varied. Radiologists and clinicians alike should be aware of this variability.
The effect of oleic acid stabilizer on the surface properties of bimetallic PtNi catalysts

Abu Bakar NH, Abu Bakar M, Bettahar MM, Ismail J, Monteverdi S

J Nanosci Nanotechnol, 2013 Jul;13(7):5034-43.
PMID: 23901527

A detailed study on the surface properties of oleic acid-stabilized PtNi nanoparticles supported on silica is reported. The oleic acid-stabilized PtNi nanoparticles were synthesized using NaBH4 as the reducing agent at various temperatures and oleic acid concentrations, prior to incorporation onto the silica support. X-ray diffraction studies of the unsupported oleic acid-stabilized PtNi particles revealed that the PtNi existed as alloys. Upon incorporation onto silica support, surface properties of the catalysts were investigated using H2-temperature reduction (H2-TPR), H2-temperature desorption (H2-TPD) and H2-chemisorption techniques. It was found that for the bimetallic catalysts, no oxides or very little oxidation occurred. Furthermore, these catalysts exhibited both Pt and Ni active sites on its surface though the availability of Ni active sites was dominant. A comparison of the surface properties of these materials with those prepared without oleic acid in our previous work [N. H. H. Abu Bakar et al., J. Catal. 265, 63 (2009)] and how they affect the hydrogenation of benzene is also discussed.
Fulltext JIS definition identified more Malaysian adults with metabolic syndrome compared to the NCEP-ATP III and IDF criteria

Ramli AS, Daher AM, Nor-Ashikin MN, Mat-Nasir N, Ng KK, Miskan M, et al.

Biomed Res Int, 2013;2013:760963.
PMID: 24175300 DOI: 10.1155/2013/760963

Metabolic syndrome (MetS) is a steering force for the cardiovascular diseases epidemic in Asia. This study aimed to compare the prevalence of MetS in Malaysian adults using NCEP-ATP III, IDF, and JIS definitions, identify the demographic factors associated with MetS, and determine the level of agreement between these definitions. The analytic sample consisted of 8,836 adults aged ≥30 years recruited at baseline in 2007-2011 from the Cardiovascular Risk Prevention Study (CRisPS), an ongoing, prospective cohort study involving 18 urban and 22 rural communities in Malaysia. JIS definition gave the highest overall prevalence (43.4%) compared to NCEP-ATP III (26.5%) and IDF (37.4%), P < 0.001. Indians had significantly higher age-adjusted prevalence compared to other ethnic groups across all MetS definitions (30.1% by NCEP-ATP III, 50.8% by IDF, and 56.5% by JIS). The likelihood of having MetS amongst the rural and urban populations was similar across all definitions. A high level of agreement between the IDF and JIS was observed (Kappa index = 0.867), while there was a lower level of agreement between the IDF and NCEP-ATP III (Kappa index = 0.580). JIS definition identified more Malaysian adults with MetS and therefore should be recommended as the preferred diagnostic criterion.
Fulltext Imaging of Osteo-odontokeratoprosthesis(OOKP) Using Multidetector Computed Tomography: A UKMMC Experience

Abu Bakar N, Lee CL

Med J Malaysia, 2013;68(1):73-5.
PMID: 23466774

Osteo-odontokeratoprosthesis (OOKP) surgery is a technique used to replace damaged cornea in blind patients for whom cadaveric transplantation is not feasible. OOKP surgery is a complex procedure requiring lifetime follow-up. The preservation of the osteo-odontolamina is the vital feature in maintaining the stability of the OOKP. Early detection of lamina resorption enables early prophylactic measures to be taken and prevent resorption-related complications. This case illustrates the radiological findings of the first OOKP surgery in Malaysia and the role of multidetector computed tomography (MDCT) in postoperative management of OOKP surgery.
Optimization of a greener method for removal phenol species by cloud point extraction and spectrophotometry

Zain NN, Abu Bakar NK, Mohamad S, Saleh NM

Spectrochim Acta A Mol Biomol Spectrosc, 2014 Jan 24;118:1121-8.
PMID: 24161875 DOI: 10.1016/j.saa.2013.09.129

A greener method based on cloud point extraction was developed for removing phenol species including 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and 4-nitrophenol (4-NP) in water samples by using the UV-Vis spectrophotometric method. The non-ionic surfactant DC193C was chosen as an extraction solvent due to its low water content in a surfactant rich phase and it is well-known as an environmentally-friendly solvent. The parameters affecting the extraction efficiency such as pH, temperature and incubation time, concentration of surfactant and salt, amount of surfactant and water content were evaluated and optimized. The proposed method was successfully applied for removing phenol species in real water samples.
Fulltext Camurati-engelmann disease association with hypogonadism and primary hypothyroidism

Low SF, Abu Bakar N, Ngiu CS

Iran Red Crescent Med J, 2014 Aug;16(8):e9481.
PMID: 25389497 DOI: 10.5812/ircmj.9481

INTRODUCTION: Camurati-Engelmann disease (CED) is a rare autosomal dominant disease with various phenotypic expressions. The symptoms usually develop during childhood. The hallmark of the disease is bilateral symmetric diaphyseal hyperostosis of the long bones with progressive involvement of the metaphysis. The epiphysis is strictly spared. The common clinical symptoms are pain of the extremities, muscle wasting, waddling gait, and lethargy. CED is rarely seen in conjunction with hypogonadism. CED-associated hypothyroidism has not been reported yet. Clinical assessment and skeletal survey are important to make the diagnosis.
CASE PRESENTATION: Hereby we reported a case of CED with concomitant hypogonadism and hypothyroidism. Serial plain radiographs of the patient showed classic and progressive diaphyseal cortical hyperostosis of the long bone.
CONCLUSIONS: Hyperostosis of the skull was observed in the present case. The characteristic osseous changes of CED were highlighted and the differential diagnoses were discussed.
Fulltext Draft Genome Sequence of Erwinia mallotivora BT-MARDI, Causative Agent of Papaya Dieback Disease

Redzuan RA, Abu Bakar N, Rozano L, Badrun R, Mat Amin N, Mohd Raih MF

Genome Announc, 2014;2(3).
PMID: 24812220 DOI: 10.1128/genomeA.00375-14

Erwinia mallotivora was isolated from papaya trees infected with dieback disease, which were planted at the Malaysian Agricultural Research and Development Institute (MARDI), Malaysia. Here, we report a draft genome sequence of E. mallotivora BT-MARDI, which offers an important source of information for understanding pathogen and host interaction during papaya dieback development.
Fulltext Molecular cloning and characterization of novel phytocystatin gene from turmeric, Curcuma longa

Chan SN, Abu Bakar N, Mahmood M, Ho CL, Shaharuddin NA

Biomed Res Int, 2014;2014:973790.
PMID: 25853138 DOI: 10.1155/2014/973790

Phytocystatin, a type of protease inhibitor (PI), plays major roles in plant defense mechanisms and has been reported to show antipathogenic properties and plant stress tolerance. Recombinant plant PIs are gaining popularity as potential candidates in engineering of crop protection and in synthesizing medicine. It is therefore crucial to identify PI from novel sources like Curcuma longa as it is more effective in combating against pathogens due to its novelty. In this study, a novel cDNA fragment encoding phytocystatin was isolated using degenerate PCR primers, designed from consensus regions of phytocystatin from other plant species. A full-length cDNA of the phytocystatin gene, designated CypCl, was acquired using 5'/3' rapid amplification of cDNA ends method and it has been deposited in NCBI database (accession number KF545954.1). It has a 687 bp long open reading frame (ORF) which encodes 228 amino acids. BLAST result indicated that CypCl is similar to cystatin protease inhibitor from Cucumis sativus with 74% max identity. Sequence analysis showed that CypCl contains most of the motifs found in a cystatin, including a G residue, LARFAV-, QxVxG sequence, PW dipeptide, and SNSL sequence at C-terminal extension. Phylogenetic studies also showed that CypCl is related to phytocystatin from Elaeis guineensis.
Fulltext Multimodality imaging of left circumflex artery to coronary sinus fistula

Sze TL, Abdul Aziz YF, Abu Bakar N, Mohd Sani F, Oemar H

Iran J Radiol, 2015 Jan;12(1):e6878.
PMID: 25793089 DOI: 10.5812/iranjradiol.6878

Coronary artery fistula (CAF) is a rare anomaly of the coronary artery. Patients with this condition are usually asymptomatic. However, cardiac failure may occur later in life due to progressive enlargement of the fistula. Diagnosis is traditionally made by echocardiogram and conventional angiogram. However with the advantage of new technologies such as computed tomography (CT) coronary angiography, the course and communications of these fistulae can be delineated non-invasively and with greater accuracy. We report a case of a left circumflex artery fistula to the coronary sinus which was suspected on echocardiogram and the diagnosis was clinched on ECG-gated CT.
Fulltext Measuring pH of the Plasmodium falciparum digestive vacuole by flow cytometry

Abu Bakar N

Trop Biomed, 2015 Sep;32(3):485-93.
PMID: 26695209 MyJurnal

Studies show that the pH of the malaria parasite's digestive vacuole (DV) plays a key role in the physiological functions of this organelle and antimalarial drug accumulation, and yet is technically difficult to measure. In this study, a flow cytometry-based technique was developed to measure the DV pH using a ratiometric pH indicator, FITC-dextran loaded into the DV of saponin-permeabilized parasites. To calculate the DV pH, a standard pH calibration curve was generated by incubating the saponin-permeabilized cells in buffers with different pH in the presence of an ionophore, CCCP. The measured average pH of the DV was 5.27 ± 0.03 that is approximately the same in the parasites observed microscopically by Hayward et al. (2006) (5.50 ± 0.14) using the same probe. The removal of glucose from the medium, causing a rapid depletion of parasite ATP, resulted in an alkalization of the DV. The DV was reacidified upon restoration of glucose to the medium. This technique provides a rapid, simple and quantitative measurement of the DV pH on a large number of cells. It will also be useful in future attempts to evaluate the effect of antimalarial drugs (i.e. chloroquine and artemisinin-based drugs) in pH changes of the DV.
Fulltext Assessment of the efficacy of CBCT referral forms at Faculty of Dentistry, International Islamic University Malaysia

Abu Bakar, N.

Malaysian Dental Journal, 2015;37(1):30-38.
MyJurnal

Introduction: Cone beam computed tomography (CBCT) has gained interest for improving diagnosis, treatment planning and facilitating better patient’s management in various dental fields. This study is part of the quality assurance programme in Dental Faculty, International Islamic University Malaysia (IIUM). This center offers dental radiography service not only for internal clinics but also act as a referral center for the eastern areas, Pahang, Malaysia. Aims: The aims of this study were; 1) to assess referral pattern for CBCT scans at the Dental Faculty, IIUM, Kuantan, and 2) to evaluate the completeness of the current referral form used by clinicians (for both internal and external referrals). Methods: An audit looking at both the external and internal referral CBCT forms of a four year period of examination (2010-2013) at the Radiology Unit, Dental Faculty, IIUM. Source of referrals, indications for CBCT and the referral forms were examined and analysed. Results: Total CBCT scans taken within the four-year period were 171, in which 96 were referred from internal sources whilst 75 were external referrals. External referrals were mainly from Paediatric Dentistry and Orthodontic clinics (59%), while other referrals were from implant clinic (20%), Paediatrics and Special Needs clinic (15%), Oral MaxilloFacial Surgery clinic (4%) and Periodontic clinic (1%) and Oral Medicine Oral Pathology (OMOP) clinics (1%). All forms from external sources are completely filled. In the Internal referral form, 41% of them came with no indication of why CBCT need to be taken. Out of 59% of cases with indications, 33% came from Paediatric Dentistry and Orthodontic department, followed by Oral Surgery department (10%). Implant treatment planning accounted for 7% of cases, Periodontics 4%, Temporo-Mandibular Joint assessment 2%, 1% for Conservative dentistry and Prosthodontic cases respectively and 1% cleft case. Further analysis of the internal referral forms revealed that only 14% of the forms were filled with complete patients’ details, 40% specified pregnancy status and 67% clearly stated patients’ medical status. Area of interest was not specified in 69% of cases and clinicians’ names were not stated in 94% of the cases. Majority of the forms were completed with clinicians’ signature (99%) and also radiographers’ details (97%). Conclusions: This study indicated that the majority of IIUM CBCT referrals were from internal sources. However, most of the referral forms were not completely filled. The internal form used, therefore, need to be improved and a much firmer referral framework should be in place.
Fulltext Electrophoretic mobility of Tween 80-encapsulated agarwood oil in aqueous

Boon, Y. T., Naim, M. N., Zakaria, R., Abu Bakar, N. F., Ahmad, N.

International Food Research Journal, 2016;23(101):133-140.
MyJurnal

A study on the feasibility of using gel electrophoresis technique in grading the agarwood oil
quality was investigated. Prior to electrophoresis, the emulsified agarwood oil droplets were
screened by a diffuse layer of ions that have equal absolute charge to that of the droplets surface
charge in aqueous phase. The condition was obtained by varying the concentrations of nonionic
surfactant; Tween 80 until the critical aggregation concentration (CAC) value of 0.0167%
(v/v) was achieved. The prepared droplets suspended in the aqueous within nano-metre size and
had ability to migrate through the agarose gel with its own specific electrophoretic mobility.
However, due to the limitation of gel pore size, only large oil droplets (>200 nm) indicated
visible bands. Overall, a novel work for grading the emulsified agarwood oil droplet with its
own electrical properties was feasible.
Fulltext Draft Genome Sequence of Blood Disease Bacterium A2 HR-MARDI, a Pathogen Causing Banana Bacterial Wilt

Badrun R, Abu Bakar N, Laboh R, Redzuan R, Bala Jaganath I

Genome Announc, 2017 Jun 01;5(22).
PMID: 28572313 DOI: 10.1128/genomeA.00408-17

Blood disease bacterium A2 HR-MARDI was isolated from banana plants infected with banana blood disease and which were planted in Kuala Kangsar, Malaysia. Here, we report a draft genome sequence of blood disease bacterium A2 HR-MARDI, which could provide important information on the virulence mechanism of this pathogen.
Ficus deltoidea reduces expression of interleukin 6 via nuclear factor kappa b pathway in human coronary artery endothelial cells

Muid S, Omar E, Ikhsan Ahmad R, Hadiani Ismail N, Alicezah Mohd Kasim N, Manaf Ali A, et al.

Atherosclerosis, 2017 Aug;263:e122.
PMID: 29365500 DOI: 10.1016/j.atherosclerosis.2017.06.392
Ficus deltoidea Trengganuensis is the most potent variant in reducing endothelial activation and monocyte-endothelial cell interaction in stimulated human coronary artery endothelial cells

Muid S, Omar E, Ahmad RI, Ismail NH, Mohd Kasim NA, Ali AM, et al.

Atherosclerosis, 2017 Aug;263:e135-e136.
PMID: 29365540 DOI: 10.1016/j.atherosclerosis.2017.06.433
Fulltext Demographics characteristics and analysis of malocclusions of orthodontic patients seen at IIUM Dental Specialist Clinic, Kuantan, Pahang

Ismail, K., Ghazali@Suhaimi, S.S., Abu Bakar, N., Mokhtar, K.I., Kharuddin, A.F.

International Medical Journal Malaysia, 2017;16(2):107-111.
MyJurnal

Introduction: Malocclusion is one of the most common dental problems observed. Limited data is currently
available regarding the demographics of malocclusion observed locally.

Materials And Methods: This is a
retrospective study where 770 patients were recruited from May 2009 till December 2014. 560 orthodontic
study models that met the inclusion criteria were examined and the malocclusions were classified according
to the British Standard Institute (BSI) incisor classification. Demographic pattern and their relationship were
analysed by structural equation modelling using SPSS (version 23.0).

Results: From 560 study models
analysed, 72% were female. The age of the patients ranges from 7-12 years old (17.1%), 13-17 years old
(55.9%) and >18 years old (27.0%). Majority of the patients were Malay followed by Chinese (5.7%), Indian
(1.8%) and other races (1.8%) Treatments received were fixed appliances (FA) (60.0%), removable appliances
(RA) (12.5%), functional appliances (FnA) (1.1%), combination of RA and FA (16.1%), combination of FnA and
FA (2.1%) and consultation (8.2%). Types of malocclusions observed were Class I (25.7%), Class II/1(32.7%),
Class II/2 (7.5%) and Class III (34.1%). There was significant association (p
Evaluation of the neurotoxic effects of chronic embryonic exposure with inorganic mercury on motor and anxiety-like responses in zebrafish (Danio rerio) larvae

Abu Bakar N, Mohd Sata NS, Ramlan NF, Wan Ibrahim WN, Zulkifli SZ, Che Abdullah CA, et al.

Neurotoxicol Teratol, 2017 Jan-Feb;59:53-61.
PMID: 27919701 DOI: 10.1016/j.ntt.2016.11.008

Chronic exposure to mercury (Hg) can lead to cumulative impairments in motor and cognitive functions including alteration in anxiety responses. Although several risk factors have been identified in recent year, little is known about the environmental factors that either due exposure toward low level of inorganic mercury that may led to the developmental disorders. The present study investigated the effects of embryonic exposure of mercury chloride on motor function and anxiety-like behavior. The embryo exposed to 6 different concentrations of HgCl2 (7.5, 15, 30, 100, 125, 250nM) at 5hpf until hatching (72hpf) in a semi-static condition. The mortality rate increased in a dose dependent manner where the chronic embryonic exposure to 100nM decreased the number of tail coiling, heartbeat, and swimming activity. Aversive stimulus was used to examine the effects of 100nM interferes with the development of anxiety-related behavior. No elevation in both thigmotaxis and avoidance response of 6dpf larvae exposed with 100nM were found. Biochemical analysis showed HgCl2 exposure affects proteins, lipids, carbohydrates and nucleic acids of the zebrafish larvae. These results showed that implication of HgCl2 on locomotor and biochemical defects affects motor performance and anxiety-like responses. Yet, the potential underlying mechanisms these responses need to be further investigated which is crucial to prevent potential hazards on the developing organism due to neurotoxicant exposure.
Fulltext Direct deposition of silver nanoplates on quartz surface by sequence pre-treatment hydroxylation and silanisation

Abu Bakar N, Mat Salleh M, Ali Umar A, Shapter JG

MethodsX, 2017;4:486-491.
PMID: 29201649 DOI: 10.1016/j.mex.2017.11.008

Silver nanoparticles deposited on quartz substrates are widely used as SERS substrates. The nanoparticles can be deposited directly from colloidal solution by dipping technique. However, the adhesion of the particles on the quartz surface is very poor. Normally the substrate is pre-treated with hydroxylation or silanisation process. In this paper, we have demonstrated that the application of the sequence pre-treatment hydroxylation and silanisation have improved the density of silver nanoplates desposited on the quartz surface. •Sequence hydroxylation and silanisation pre-treatment assists the deposition of the nanoplate on the surface.•Various immersion times of the quartz surface into the colloidal nanoplates determined size distributions and density surface of the nanoplates on the surface.
Fulltext Clinical glycomics for the diagnosis of congenital disorders of glycosylation

Abu Bakar N, Lefeber DJ, van Scherpenzeel M

J Inherit Metab Dis, 2018 May;41(3):499-513.
PMID: 29497882 DOI: 10.1007/s10545-018-0144-9

Clinical glycomics comprises a spectrum of different analytical methodologies to analyze glycan structures, which provides insights into the mechanisms of glycosylation. Within clinical diagnostics, glycomics serves as a functional readout of genetic variants, and can form a basis for therapy development, as was described for PGM1-CDG. Integration of glycomics with genomics has resulted in the elucidation of previously unknown disorders of glycosylation, namely CCDC115-CDG, TMEM199-CDG, ATP6AP1-CDG, MAN1B1-CDG, and PGM1-CDG. This review provides an introduction into protein glycosylation and presents the different glycomics methodologies ranging from gel electrophoresis to mass spectrometry (MS) and from free glycans to intact glycoproteins. The role of glycomics in the diagnosis of congenital disorders of glycosylation (CDG) is presented, including a diagnostic flow chart and an overview of glycomics data of known CDG subtypes. The review ends with some future perspectives, showing upcoming technologies as system wide mapping of the N- and O-glycoproteome, intact glycoprotein profiling and analysis of sugar metabolism. These new advances will provide additional insights and opportunities to develop personalized therapy. This is especially true for inborn errors of metabolism, which are amenable to causal therapy, because interventions through supplementation therapy can directly target the pathogenesis at the molecular level.

Filters

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links