MyMedR

Inheritance of haemoglobin H disease. A new aspect

Lie-Injo LE, Lopez CG, Lopes M

Acta Haematol., 1971;46(2):106-20.

PMID: 4331171 DOI: 10.1159/000208565

A study of 23 patients with Hb H disease and their 82 relatives in 17 families showed that 2 types of this condition exist. One is associated with the presence of a small slow-moving component, which we tentatively called the X component and which was invariably present in one parent. Some siblings also had it. The other type was not associated with this component. Two patients without X component had a newborn with Bart’s haemoglobin without X component. None of the parents of 20 newborns with Hb Bart’s without the X component had the X component. It was present in only one parent of each of 2 newborns with Hb Bart’s and the X component. They are thought to represent Hb H disease in the newborn period. We suggest that at least 3 abnormal genes may lead to Hb H disease, which results when 2 of the 3 combine. Severity of clinical and haematological symptoms depends upon which abnormal gene is present and which 2 are involved in any particular combination.
Key Words: a-Thalassaemia; Haemoglobin Bart’s; Haemoglobin H disease; Haemoglobinopathies

Matched MeSH terms: Chromatography, DEAE-Cellulose

Unusual albumin variants in Indonesians and Malayan aborigines

Lie-Injo Luan Eng, Weitkamp LR, Kosasih EN, Bolton JM, Moore CL

Hum. Hered., 1971;21(4):376-83.

PMID: 5003129

Matched MeSH terms: Cellulose

Structural identification of haemoglobin F Kuala Lumpur: alpha2 gamma2 22(B4)Asp leads to Gly; 136 Ala

Luan Eng LI, Wiltshire BG, Lehmann H

Biochim. Biophys. Acta, 1973 Oct 18;322(2):224-30.

PMID: 4765089

Matched MeSH terms: Cellulose

Haemoglobin Bart's and slow-moving haemoglobin x components in newborns. The homozygous state for the slow-moving X components in a Malay boy

Lie-Injo LE

Acta Haematol., 1973;49(1):25-35.

PMID: 4632449 DOI: 10.1159/000208382

Newborns were examined for the presence of slow-moving haemoglobin components, tentatively designated X components and previously found in a group of Hb H disease in which invariably one of the parents of each patient had the same slow-moving Hb X components also. Structural studies showed that the abnormal haemoglobin in Chinese was identical with Hb Constant Spring, an c-chain variant. Newborns with Hb Bart’s and slow-moving X components invariably had one parent with the X components also. When the child grew older Hb Bart’s disappeared while the Hb X components remained in the blood. The homozygous state for the X components was found in a Malay boy through his newborn brother who had the X components in addition to Hb Bart’s and had both parents with the X components. One other Malay baby had the X components and Hb A2 Indonesia inherited from the parents. The present study of newborns also showed that Hb Bart’s can accompany different abnormalities of haemoglobin production, involving alpha-chains, beta-chains as well as gamm-chains. Its presence in cord blood is, therefore, not specific for alpha-thalassaemia
Key Words: Haemoglobinopathies; Hb Bart’s; Slow-moving Hb X; Thalassaemia

Matched MeSH terms: Cellulose; Chromatography, DEAE-Cellulose

Multiple fetal and adult hemoglobins in Malaysian Macaca nemestrina: consequences of a-chain variation

Nute PE, Pataryas HA

Am. J. Phys. Anthropol., 1974 Jan;40(1):17-25.

PMID: 4206325

Matched MeSH terms: Chromatography, DEAE-Cellulose

Homozygous state for Hb Constant Spring (slow-moving Hb X components)

Lie-Injo LE, Ganesan J, Clegg JB, Weatherall DJ

Blood, 1974 Feb;43(2):251-9.

PMID: 4810076

Matched MeSH terms: Cellulose

Haemoglobin F Malaysia: alpha 2, gamma 2 1(NA1) glycine--cysteine; 136 glycine

Eng LI, Kamuzora H, Lehmann H

J Med Genet, 1974 Mar;11(1):25-30.

PMID: 4837284

Matched MeSH terms: Chromatography, DEAE-Cellulose

Caseinolytic and esteratic activities of Malayan pit viper venom and its proteolytic and thrombin-like fractions

Soh KS, Chan KE

Toxicon, 1974 Mar;12(2):151-8.

PMID: 4859238

Matched MeSH terms: Chromatography, DEAE-Cellulose

Isolation and characterization of the major phospholipase A2 from the venom of Trimeresurus purpureomaculatus (shore pit viper)

Nget Hong Tan, Chon Seng Tan, Hun Teck Khor

Int. J. Biochem., 1989;21(12):1421-6.

PMID: 2612728

1. The major phospholipase A2 (PLA-DE4) of the venom of Trimeresurus purpureomaculatus (shore pit viper) has been purified to electrophoretic homogeneity. 2. The isoelectric point of the purified enzyme was determined to be 4.20, and the mol. wt was 31,700 as estimated by Sephadex G-75 gel filtration chromatography; and 14,000 as estimated by SDS-polyacrylamide gel electrophoresis. The purified enzyme hydrolyzed phosphatidylcholine (PC) faster than phosphatidylethanolamine (PE), whereas phosphatidylserine (PS) was not hydrolyzed at all (PC greater than PE greater than PS =0). However, in reaction system consisted of mixtures of PC and PS, phosphatidylserine was effectively hydrolyzed by the enzyme. 4. The phospholipase A2 exhibited edema-forming activity but not hemolytic, hemorrhagic or anticoagulant activities. It was not lethal to mice at a dosage of 10 micrograms/g by i.v. route.

Matched MeSH terms: Chromatography, DEAE-Cellulose

Hydrops fetalis: a simple novel screen for the rapid identification of haemoglobin Bart's syndrome

George-Kodiseri E, Faridah K

Family Physician, 1991;3(1):25-27.

Haemoglobin Bart's hydrops fetalis syndrome is totally lethal. Globin chain electrophoresis on mylar backed cellulose acetate strips, by a method modified from Ueda and Schneider has been established to demonstrate total absence of alpha chains in this syndrome. This simple test can identify fetuses, stillbirths and newborns with homozygous Î±o-thalassaemia. In this region where DNA studies are limited, and prenatal diagnosis is unavailable, this test which describes the phenotypic expression of Hb Bart's syndrome will improve genetic counselling of women at risk of homozygous Î±o-thalassaemia.

Matched MeSH terms: Cellulose

Effects of thiram and terbuthylazine on cellulose decomposition in two soils

Sahid I, Razlin W, Zaabar W

Bull Environ Contam Toxicol, 1993 Oct;51(4):605-11.

PMID: 8400666

Matched MeSH terms: Cellulose/metabolism*

Effects of two acetanilide herbicides on microbial populations and their cellulolytic activities

Sahid IB, Yap MY

Bull Environ Contam Toxicol, 1994 Jan;52(1):61-8.

PMID: 8130418

Matched MeSH terms: Cellulose/metabolism*

Genetic studies of water buffalo blood markers. II. Carbonic anhydrase, esterase D, malate dehydrogenase, and malic enzyme

Sreetharan K, Mukherjee TK, Tan SG, Selvaraj OS, Barker JS

Biochem Genet, 1994 Feb;32(1-2):35-8.

PMID: 8031293

Matched MeSH terms: Electrophoresis, Cellulose Acetate

A study on the effect of operating parameters on the cross-flow microfiltration of yeasts

Hashim MA, Sen Gupta B

Bioseparation, 1997;7(1):17-23.

PMID: 9615610

The effects of pump speed, cumulative permeate volume and concentration of feed (yeast cells) on the permeate flux have been studied on a batch cross-flow microfiltration process. The experiments were conducted for two different cellulose acetate membrane modules of 0.2 micron and 0.45 micron pore size. A three factor experiment was designed for this purpose and the effect of the operating parameters on the filtration rate was studied by the analysis of variance (ANOVA). It is concluded from the analysis of the experimental data that pump speed has the maximum bearing upon the permeate rate within the operating range of parameters. Fouling conditions were examined in the light of colloids deposition on membranes due to surface interactions. However this paper looks into the relationship and sensitivity of the operating parameters in a cross-flow microfiltration unit rather than exploring the theoretical principles behind the observed phenomena.

Matched MeSH terms: Cellulose/analogs & derivatives

Use of artificial neural networks to predict drug dissolution profiles and evaluation of network performance using similarity factor

Peh KK, Lim CP, Quek SS, Khoh KH

Pharm Res, 2000 Nov;17(11):1384-8.

PMID: 11205731

PURPOSE: To use artificial neural networks for predicting dissolution profiles of matrix-controlled release theophylline pellet preparation, and to evaluate the network performance by comparing the predicted dissolution profiles with those obtained from physical experiments using similarity factor.

METHODS: The Multi-Layered Perceptron (MLP) neural network was used to predict the dissolution profiles of theophylline pellets containing different ratios of microcrystalline cellulose (MCC) and glyceryl monostearate (GMS). The concepts of leave-one-out as well as a time-point by time-point estimation basis were used to predict the rate of drug release for each matrix ratio. All the data were used for training, except for one set which was selected to compare with the predicted output. The closeness between the predicted and the reference dissolution profiles was investigated using similarity factor (f2).

RESULTS: The f2 values were all above 60, indicating that the predicted dissolution profiles were closely similar to the dissolution profiles obtained from physical experiments.

CONCLUSION: The MLP network could be used as a model for predicting the dissolution profiles of matrix-controlled release theophylline pellet preparation in product development.

Matched MeSH terms: Cellulose/chemistry

Fulltext High performance liquid chromatography (HPLC) as a screening tool for classical Beta-thalassaemia trait in malaysia

George E, Jamal AR, Khalid F, Osman KA

Malays J Med Sci, 2001 Jul;8(2):40-6.

PMID: 22893759 MyJurnal

Beta-thalassaemia is characterized by a decrease (β(+)) or absence (β(0)) in the synthesis of β-globin chains of human haemoglobin. The heterozygous state for β(+) or β(0) result in β-thalassaemia trait in which the hallmark is the presence of an elevated level of Haemoglobin (Hb) A(2) (α(2)δ(2)). In the past, the traditional methods such as cellulose acetate electrophoresis with elution and microcolumn chromatrography have been the techniques used by the majority of the laboratories in Malaysia for the estimation of (Hb) A(2) levels. The recommended method currently is high performance liquid chromatography which has only been introduced in a few laboratories in the country.

Matched MeSH terms: Electrophoresis, Cellulose Acetate

Comparison on pore development of activated carbon produced from palm shell and coconut shell

Daud WM, Ali WS

Bioresour Technol, 2004 May;93(1):63-9.

PMID: 14987722

A series of experiments were conducted to compare the pore development in palm-shell and coconut-shell-based activated carbons produced under identical experimental conditions. Carbonization and activation processes were carried out at 850 degrees C using a fluidized bed reactor. Within the range of burn-off studied, at any burn-off, the micropore and mesopore volumes created in palm-shell-based activated carbon were always higher than those of coconut-shell-based activated carbon. On macropore volume, for palm-shell-based activated carbon, the volume increased with increase in burn-off up to 30% and then decreased. However, for coconut-shell-based activated carbon, the change in macropore volume with burn-off was almost negligible but the absolute macropore volume decreased with burn-off.

Matched MeSH terms: Cellulose/metabolism

Comparison studies on the percolation thresholds of binary mixture tablets containing excipients of plastic/brittle and plastic/plastic deformation properties

Amin MC, Fell JT

Drug Dev Ind Pharm, 2004;30(9):937-45.

PMID: 15554218

Percolation theory has been used with great interest in understanding the design and characterization of dosage forms. In this study, work has been carried out to investigate the behavior of binary mixture tablets containing excipients of similar and different deformation properties. The binary mixture tablets were prepared by direct compression using lactose, polyvinyl chloride (PVC), Eudragit RS 100, and microcrystalline cellulose (MCC). The application of percolation theory on the relationships between compactibility, Pmax, or compression susceptibility (compressibility), gamma, and mixture compositions reveals the presence of percolation thresholds even for mixtures of similar deformation properties. The results showed that all mixture compositions exhibited at least one discreet change in the slope, which was referred to as the percolation threshold. The PVC/Eudragit RS100 mixture compositions showed significant percolation threshold at 80% (w/w) PVC loading. Two percolation thresholds were observed from a series of binary mixtures containing similar plastic deformation materials (PVC/MCC). The percolation thresholds were determined at 20% (w/w) and 80% (w/w) PVC loading. These are areas where one of the components percolates throughout the system and the properties of the tablets are expected to experience a sudden change. Experimental results, however, showed that total disruption of the tablet physical properties at the specified percolation thresholds can be observed for PVC/lactose mixtures at 20-30% (w/w) loading while only minor changes in the tablets' strength for PVC/MCC or PVC/Eudragit RS 100 mixtures were observed.

Matched MeSH terms: Cellulose/chemistry

Solid state bioconversion of oil palm biomass for ligninase enzyme production

Alam MZ, Mahmat ME, Muhammad N

Artif Cells Blood Substit Immobil Biotechnol, 2005;33(4):457-66.

PMID: 16317964

A laboratory-scale study of bioconversion of local lignocellulosic material, oil palm biomass (OPB) was conducted by evaluating the enzyme production through microbial treatment in solid state bioconversion (SSB). OPB in the form of empty fruit bunches (EFB) was used as a solid substrate and treated with the white-rot fungus, Phanerochaete chrysosporium, to produce ligninase. The results showed that the highest ligninase activity of 400.27 U/liter was obtained at day 12 of fermentation. While the optimum study indicated the enzyme production of 1472.8 U/liter with moisture content of 50%, 578.7 U/liter with 10% v/w of inoculum size, and 721.8 U/liter with co-substrate concentration of 1% (w/w) at days 9, 9 and 12 of fungal treatment, respectively. The parameters glucosamine and reducing sugar were observed to evaluate the growth and substrate utilization in the experiment.

Matched MeSH terms: Cellulose/metabolism

Fungal solid state culture of palm kernel cake

Iluyemi FB, Hanafi MM, Radziah O, Kamarudin MS

Bioresour Technol, 2006 Feb;97(3):477-82.

PMID: 16216731

Palm kernel cake (PKC), an agro-industrial by-product used extensively in the animal feed industry, has limited use in fish feeds due to its high fiber and low protein contents. In this study, PKC was processed under solid state culture conditions with five fungal strains and the effect of this fungal culturing on the amino acid, fatty acid, cellulose and hemicellulose fractions was evaluated. Fungal strains used were Sclerotium rolfsii, Trichoderma harzianum, Trichoderma longiobrachiatum, Trichoderma koninggi and Aspergillus niger. Fungal growth was carried out at 50% moisture level and 1% inoculum level for 7 days. A significant increase in protein content from 18.76% to 32.79% was obtained by growing T. longibrachiatum on PKC. Cellulose level decreased significantly from 28.31% to 12.11% for PKC cultured with T. longibrachiatum, and hemicellulose from 37.03% to 19.01% for PKC cultured with A. niger. Fungal culturing of PKC brought about an increase in the level of unsaturated- and a decrease in the level of the saturated-fatty acids.

Matched MeSH terms: Cellulose/analysis

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