Displaying publications 201 - 220 of 1919 in total

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  1. Long term visit-to-visit variability of systolic blood pressure and cardiovascular disease events in a primary care setting: a 10-year retrospective cohort study [Abstract OS 04-05]
    Ching SM, Chia YC, Lim HM
    J Hypertens, 2016 Sep;34 Suppl 1 - ISH 2016 Abstract Book:e56.
    PMID: 27753921
    Conference abstract:
    OBJECTIVE: This study aims to determine the relationship of long term visit to visit variability (VVV) of SBP and cardiovascular disease (CVD) in a primary care setting.
    DESIGN AND METHOD: This is a retrospective study of a cohort of 1416 patients over a period of 10 years (1998-2007). Demographic data, three monthly clinic BP readings and CVD events were captured from patient records. We derived the mean BP and VVV of SBPs for each subject and divided them into three groups defined as non hypertension, developed hypertension along the 10-year follow-up and persistent hypertension. We examined differences in cardiovascular events across these groups.
    RESULTS: Mean age of the participants at baseline was 56.5 ± 10.1 years, 34.6% were males. Table 1 describes mean SBP, BPV and CVD events of the study population.Those with both low mean SBP and low BPV have the lowest CVD events, conversely those with both high mean SBP and high BPV have highest CVD events. In those patients with the same mean SBP, whether high or low, those with higher BPV have more events than those with lower BPV. However patients with low mean SBP but high BPV have more CVD events compared with those patients with high mean SBP but low BPV (p = 0.04) suggesting BPV is more important than mean SBP in causing CVD events.We used ROC of VVV SBP to identify the cut off point of 12.9 mmHg as the indicator for increase in CVD events.
    CONCLUSIONS: In our study, we found that patients with hypertension have higher BPV than normotensive subjects. Furthermore those with higher BPV also had more CVD events. As such, we should prioritize lowering not only mean systolic BP but lowering BPV as well. Long term VVV SBP should be another target in the management of patients with hypertension.
    Matched MeSH terms: Blood Pressure*
  2. Analysis of 1,2(2,3)- and 1,3-positional isomers of diacylglycerols from vegetable oils by reversed-phase high-performance liquid chromatography
    Lo SK, Baharin BS, Tan CP, Lai OM
    J Chromatogr Sci, 2004 Mar;42(3):145-54.
    PMID: 15023251
    Separation of 1,2(2,3)- and 1,3-positional isomers of diacylglycerols (DAG) from vegetable oils by reversed-phase high-performance liquid chromatography (RP-HPLC) is investigated. The method is based on isocratic elution using 100% acetonitrile and UV detection at 205 nm. The following elution order of DAG molecular species is identified: 1,3-dilinolein < 1,2-dilinolein < 1,3-dimyristin < 1-oleoyl-3-linoleoyl-glycerol < 1,2-dimyristoyl-rac-glycerol < 1(2)-oleoyl-2(3)-linoleoyl-glycerol < 1-linolenoyl-3-stearoyl-glycerol < 1(2)-linolenoyl-2(3)-stearoyl-glycerol < 1,3-diolein < 1-palmitoyl-3-oleoyl-glycerol < 1,2-dioleoyl-sn-glycerol < 1(2)-palmitoyl-2(3)-oleoyl-glycerol < 1-linoleoyl-3-stearoyl-glycerol < 1,3-dipalmitin < 1(2)-linoleoyl-2(3)-stearoyl-glycerol < 1-oleoyl-3-stearoyl-glycerol < 1,2-dipalmitoyl-rac-glycerol < 1-palmitoyl-3-stearoyl-sn-glycerol < 1,3-distearin < 1,2-distearoyl-rac-glycerol. Linearity is observed over three orders of magnitude. Limits of detection and quantitation range 0.2-0.7 microg/mL for 1,3-dilinolein to 0.6-1.9 microg/mL for 1,2-dioleoyl-sn-glycerol, respectively. Precision and accuracy of the method are also demonstrated. The method is developed to separate mixtures of DAG molecular species produced from edible oils.
    Matched MeSH terms: Chromatography, High Pressure Liquid/methods*
  3. Modulation of intraocular pressure by unilateral and forced unilateral nostril breathing in young healthy human subjects
    Mohan SM, Reddy SC, Wei LY
    Int Ophthalmol, 2001;24(6):305-11.
    PMID: 14750567
    PURPOSE: To determine the effects of unilateral right/left nostril breathing (URNB/ULNB) and forced unilateral right/left nostril breathing (FURNB/FULNB) on intraocular pressure (IOP) and to examine the differences in the IOP during the various phases of nasal cycle.

    METHODS: Young healthy volunteers of either sex aged between 19-24 years, participated in the sessions using URNB/ULNB (n = 52) and FURNB/FULNB (n = 28). The nostril dominance was calculated from signals recorded on the PowerLab equipment, representing pressure changes at the end of the nostrils during respiration. The IOP was measured with Tono-Pen. The subjects were divided into 4 groups viz. right nostril dominant (RND), left nostril dominant (LND), transitional right nostril dominant (TRND) and transitional left nostril dominant (TLND) groups. The IOP data 'before and after' URNB/ULNB or FURNB/FULNB were compared by using paired t-test. The baseline data of IOP between the groups were analysed by using independent samples t-test.

    RESULTS: The URNB decreased the IOP in the LND and TLND (p < 0.01) and also in the RND (p < 0.05) groups but not significantly in the TRND group. The ULNB decreased the IOP in the RND group (p < 0.01) only. The FURNB significantly reduced the IOP (p < 0.05) only in the LND and RND groups. The FULNB decreased the IOP but not significantly. The baseline IOP did not differ significantly between the LND, RND, TLND and TRND groups.

    CONCLUSION: The URNB/FURNB reduced the IOP, while ULNB/FULNB failed to increase the IOP significantly. It is suggested that the lowering of IOP by URNB indicated sympathetic stimulation.

    Matched MeSH terms: Intraocular Pressure/physiology*
  4. Ganglioside Composition in Beef, Chicken, Pork, and Fish Determined Using Liquid Chromatography-High-Resolution Mass Spectrometry
    Fong BY, Ma L, Khor GL, van der Does Y, Rowan A, McJarrow P, et al.
    J Agric Food Chem, 2016 Aug 17;64(32):6295-305.
    PMID: 27436425 DOI: 10.1021/acs.jafc.6b02200
    Gangliosides (GA) are found in animal tissues and fluids, such as blood and milk. These sialo-glycosphingolipids have bioactivities in neural development, the gastrointestinal tract, and the immune system. In this study, a high-performance liquid chromatography-mass spectrometry (HPLC-MS) method was validated to characterize and quantitate the GA in beef, chicken, pork, and fish species (turbot, snapper, king salmon, and island mackerel). For the first time, we report the concentration of GM3, the dominant GA in these foods, as ranging from 0.35 to 1.1 mg/100 g and 0.70 to 5.86 mg/100 g of meat and fish, respectively. The minor GAs measured were GD3, GD1a, GD1b, and GT1b. Molecular species distribution revealed that the GA contained long- to very-long-chain acyl fatty acids attached to the ceramide moiety. Fish GA contained only N-acetylneuraminic acid (NeuAc) sialic acid, while beef, chicken, and pork contained GD1a/b species that incorporated both NeuAc and N-glycolylneuraminic acid (NeuGc) and hydroxylated fatty acids.
    Matched MeSH terms: Chromatography, High Pressure Liquid/methods*
  5. Fulltext Intervention for Diabetes with Education, Advancement and Support (IDEAS) study: protocol for a cluster randomised controlled trial
    Lee JY, Chan CK, Chua SS, Ng CJ, Paraidathathu T, Lee KK, et al.
    BMC Health Serv Res, 2016 Sep 29;16(1):524.
    PMID: 27683021 DOI: 10.1186/s12913-016-1782-y
    BACKGROUND: The high market penetration of mobile phones has triggered an opportunity to combine mobile technology with health care to overcome challenges in today's health care setting. Although Malaysia has a high Internet and mobile penetration rate, evaluations of the efficacy of incorporating this technology in diabetes care is not common. We report the development of a telemonitoring coaching system, using the United Kingdom (UK) Medical Research Council (MRC) framework, for patients with type 2 diabetes mellitus.

    METHODS: The Intervention for Diabetes with Education, Technological Advancement and Support (IDEAS) study is a telemonitoring programme based on an empowerment philosophy to enable participants to be responsible for their own health decision and behaviour. An iterative cycle of development, piloting, and collating qualitative and quantitative data will be used to inform and refine the intervention. To increase compliance, the intervention will be designed to encourage self-management using simple, non-technical knowledge. The primary outcomes will be HbA1c, blood pressure, total cholesterol, and quality of life and diabetes self-efficacy. In addition, an economic analysis on health service utilisation will be collected.

    DISCUSSION: The mixed-method approach in this study will allow for a holistic overview of using telemonitoring in diabetes care. This design enables researchers to understand the effectiveness of telemonitoring as well as provide insights towards the receptiveness of incorporating information technology amongst type 2 diabetes patients in a community setting.

    TRIAL REGISTRATION: ClinicalTrials.gov NCT02466880 Registered 2 June 2015.
    Matched MeSH terms: Blood Pressure; Blood Pressure Determination
  6. Fulltext Weights and blood pressures of women who attend family planning clinics in Sarawak
    Wadsworth GR
    Med J Malaysia, 1981 Sep;36(3):148-50.
    PMID: 7329371
    Matched MeSH terms: Blood Pressure*
  7. Fulltext Blood pressure tracking as an indicator of hypertension risk
    Zulkifli SN
    Singapore Med J, 1992 Oct;33(5):474-7.
    PMID: 1455271
    The significance of high blood pressure to cardio- and cerebrovascular disease is well recognized. The associated mortality and morbidity risks call for multiple approaches to control the development of high blood pressure. Recent studies have shown that precursors of cardiovascular disorders may be apparent from a young age, and that these can persist and contribute to disease in later life. With regard to blood pressure, the phenomenon of persisting at the same rank has been referred to as tracking. This allows for the possible identification of high-risk groups, for whom early intervention can be initiated. In this paper, some of the findings of studies on blood pressure tracking are reviewed.
    Matched MeSH terms: Blood Pressure*
  8. Form and habit in species of malleus (including the "hammer oysters") with comparative observations on Isognomon isognomon
    Yonge CM
    Biol. Bull., 1968 Oct;135(2):378-405.
    PMID: 28368764 DOI: 10.2307/1539789
    1. Study of the structure and mode of life of Malleus regula provides the basis for consideration of the "hammer" species, M. malleus and M. albus.
    2. M. regula occurs byssally attached, vertically disposed on rocky substrates associated with mud and is widely distributed in the tropical Indo-Pacific.
    3. The distal two-thirds of the elongated shell is exclusively prismatic. By means of special pallial retractors the mantle lobes can be withdrawn within the nacreous region.
    4. The massive opisthodetic ligament has a short secondary extension of fusion layer.
    5. A promyal chamber on the right side proximal to the adductor increases water flow into the exhalant chamber.
    6. The long filibranch ctenidia provide a vertically extended food-collecting surface.
    7. The foot is concerned with planting of the massive byssus which emerges through a notch in the right valve but on the under ( i.e., dorsal) surface. There is also a unique and very long accessory foot, ventrally grooved, everywhere ciliated and in constant writhing activity due to blood pressure and intrinsic muscle. Moving freely throughout the lower mantle cavity it can only be concerned with cleansing.
    8. Pseudofaeces are ejected from the distal tip of the mantle cavity.
    9. M. malleus, the black hammer shell, occurs vertically embedded in coarse sand or sandy gravel. Byssus threads are attached to fragments within the substrate. The great anterior and posterior elongations of the hinge line (also exclusively prismatic) are separated by the byssal notch (now affecting both valves).
    10. Shells are usually excessively irregular due to the great exposure to damage and the almost unlimited powers of rapid repair by the three pallial extensions.
    11. M. albus, the white hammer shell, is stouter and inhabits muddy sand. During growth it loses the byssus with reduction of the foot and byssal retractors and closure of the byssal notch. The accessory foot is not affected. The animal becomes anchored in the substrate exclusively by the anterior and posterior extensions of the shell.
    12. Description of the typically rounded Isognomon ephippium leads to that of the elongated I. isognomon which occupies precisely the same habitat as M. regula.
    13. It lacks the pallial retractors, promyal chamber and accessory foot of Malleus, the nacreous region is more extended distally and the ligament is multivincular (the formation of which is discussed), producing some posterior, but never any anterior, extension of the hinge line.
    14. A remarkably dense mixed bed of M. regula and I. isognomon in Darvel Bay, Sabah (Borneo), is described. Different spawning periods, by preventing competition during settlement, may account for this complete intermixing of sympatric species, which, however, are generalized herbivores where numbers are not limited by food supply.
    15. From a basic epifaunal habit (Pteria, Isognomon, M. regula), members of the Pteriacea have become adapted for infaunal life within sponges (Vulsella, Crenatula) or within soft substrates ( M. malleus, M. alba and the Pinnidae).
    16. There is final discussion about elongation in monomyarians, i.e., in the genus Malleus and in I. isognomon.
    Matched MeSH terms: Blood Pressure; Blood Pressure Determination
  9. EEG-based alpha neurofeedback training for mood enhancement
    Phneah SW, Nisar H
    Australas Phys Eng Sci Med, 2017 Mar 13.
    PMID: 28290068 DOI: 10.1007/s13246-017-0538-2
    The aim of this paper is to develop a preliminary neurofeedback system to improve the mood of the subjects using audio signals by enhancing their alpha brainwaves. Assessment of the effect of music on the human subjects is performed using three methods; subjective assessment of mood with the help of a questionnaire, the effect on brain by analysing EEG signals, and the effect on body by physiological assessment. In this study, two experiments have been designed. The first experiment was to determine the short-term effect of music on soothing human subjects, whereas the second experiment was to determine its long-term effect. Two types of music were used in the first experiment, the favourite music selected by the participants and a relaxing music with alpha wave binaural beats. The research findings showed that the relaxing music has a better soothing effect on the participants psychologically and physiologically. However, the one-way analysis of variance (ANOVA) results showed that the short-term soothing effect of both favourite music and relaxing music was not significant in changing the mean alpha absolute power and mean physiological measures (blood pressure and heart rate) at the significance level of 0.05. The second experiment was somewhat similar to an alpha neurofeedback training whereby the participants trained their brains to produce more alpha brainwaves by listening to the relaxing music with alpha wave binaural beats for a duration of 30 min daily. The results showed that the relaxing music has a long-term psychological and physiological effect on soothing the participants, as can be observed from the increase in alpha power and decrease in physiological measures after each session of training. The training was found to be effective in increasing the alpha power significantly [F(2,12) = 11.5458 and p = 0.0016], but no significant reduction in physiological measures was observed at the significance level of 0.05.
    Matched MeSH terms: Blood Pressure; Blood Pressure Determination
  10. Fulltext Innovative Approaches to Hypertension Control in Low- and Middle-Income Countries
    Vedanthan R, Bernabe-Ortiz A, Herasme OI, Joshi R, Lopez-Jaramillo P, Thrift AG, et al.
    Cardiol Clin, 2017 Feb;35(1):99-115.
    PMID: 27886793 DOI: 10.1016/j.ccl.2016.08.010
    Elevated blood pressure, a major risk factor for ischemic heart disease, heart failure, and stroke, is the leading global risk for mortality. Treatment and control rates are very low in low- and middle-income countries. There is an urgent need to address this problem. The Global Alliance for Chronic Diseases sponsored research projects focus on controlling hypertension, including community engagement, salt reduction, salt substitution, task redistribution, mHealth, and fixed-dose combination therapies. This paper reviews the rationale for each approach and summarizes the experience of some of the research teams. The studies demonstrate innovative and practical methods for improving hypertension control.
    Matched MeSH terms: Blood Pressure*
  11. Fulltext Diabetes control - the legacy of a memory
    Vijay AP, Chan SP
    JUMMEC, 2009;12(2):47-56.
    MyJurnal
    Achieving and maintaining good glycaemic control remains an important goal in the management of this common and prevalent disorder. Recent evidence from important megatrials, ACCORD, ADVANCE, VADT, UKPDS-10 year follow-up as well as the STENO-2 follow-up study, have cleared doubts concerning the benefits of targeting good glycaemic control. For the first time, we have the reassurance that macrovascular benefits can be realised from good glycaemic control. The legacy effect of prior good glucose control from the UKPDS-10 year follow-up, reinforces the results seen from the DCCT-EDIC (for Type 1 diabetes). The Intervention Phase of the UKPDS revealed benefits for reduction of microvascular complications, while it was only at the end of the Post-Trial Monitoring Phase where significant improvements in both micro and macrovascular outcomes were seen. The other three Trials assessing the effect of glycaemic control on cardiovascular outcomes, although largely negative for CV benefit, give valuable insight towards appropriate patient characteristics for which aggressive glucose control can and should be instituted. Individualising glycaemic targets, which has been the approach that many clinicians have been practising, has received new impetus albeit with clearer details. Getting to glycaemic goal early in the course of T2DM and Doing to Safely (Avoiding hypoglycaemia)are the key ingredients to successful management. The legacy of the memory of initial good metabolic/glycaemic control is investment in good health with benefits of reductions in both micro and more importantly, macrovascular disease, years later. Multifactorial interventions that include blood pressure, lipid lowering in addition to glucose control in these individuals with the Metabolic Syndrome result in more immediate beneficial additive effects on cardiovascular outcomes.
    Matched MeSH terms: Blood Pressure; Blood Pressure Determination
  12. Fulltext Occupational lead exposure of soldering workers in an electronic factory
    Mimala Arasaratnam, Zailina Hashim, Shamsul Bahari Shamsudin
    Journal of Occupational Safety and Health, 2004;1(2):39-55.
    MyJurnal
    A cross-sectional study was conducted on 83 female electronics factory workers. The respondents comprised 50 exposed workers who use lead alloy solder and 33 unexposed workers. The objective of this study was to assess the lead exposure of these workers. Breathing zone were sampled using air sampling pumps. Dust samples were collected by wipe method. Venous blood collected and blood pressure were measured. All lead analyses were carried out with Graphite Furnace Atomic Absorption Spectrophotometer. The mean air lead for exposed workers (57 0. ± 0.93 μg/m³) was significantly higher than the unexposed workers (0.0067 ± 0.0045μg/m³) (p
    Matched MeSH terms: Blood Pressure; Blood Pressure Determination
  13. Normobaric hyperoxia treatment in traumatic brain injury: a focus on basal ganglia
    Fatin Azwa Haruddin
    Orient Neuron Nexus, 2010;1(1):13-16.
    MyJurnal
    Traumatic brain injury (TBI) is known to inflict significant morbidity and mortality worldwide. In severe TBI cases, the resulting physical and cognitive impairments incur high management and rehabilitation costs that crucially involve monitoring intracranial pressure (ICP) and improving brain oxygenation. Normobaric Hyperoxia Treatment (NBOT) is a therapeutic strategy to improve brain oxygen metabolism and to decrease ICP by reducing tissue swelling and deactivating toxin. NBOT is administered by increasing the inspired oxygen concentration to 100% in normal atmospheric pressure. Previous studies involving NBOT had explored its effectiveness to salvage the TBI-related cognitive and motor deficits. However, the focus of these studies has frequently been on the cortical lesions despite the known facts that TBI often inflicts tissue damage to the subcortical areas such as the basal ganglia. There are growing evidence to support recent functional theories that implicate a pivotal role of the basal ganglia in regulating normal movements and cognition through dopamine (DA) and glutamate interaction. Thus, tissue damages leading to TBI-related motor and cognitive deficits may involve the different affected brain regions. This minireview attempts to highlight the key processes involved in the pathophysiology of severe TBI and offers insights into the role of NBOT by exploring its potential effects on the cerebral energy metabolism and gene expression patterns of dopamine receptor in a mouse model.
    Matched MeSH terms: Atmospheric Pressure; Intracranial Pressure
  14. Splenomegaly and blood pressure in an Orang Asli community in West Malaysia
    Burns-Cox CJ
    Am Heart J, 1970 Nov;80(5):718-9.
    PMID: 5474109
    Matched MeSH terms: Blood Pressure*
  15. Coagulation of Malays and caucasians: a comparative study of two groups of young soldiers
    Loncin H
    Med J Malaya, 1965 Sep;20(1):85-7.
    PMID: 4221440
    Matched MeSH terms: Blood Pressure*
  16. Fulltext Blood pressure patterns and the prevalence of hypertension and its associated factors in a rural community in Northern Malaysia
    Narayan, K.A., A Rashid, K.
    Malaysian Journal of Public Health Medicine, 2007;7(1):14-19.
    MyJurnal
    This study was conducted to study the blood pressure pattern and the prevalence of hypertension and its associated factors in a rural community in two coastal villages in rural Kedah, Mahysia. Out of the total population 504 were above 20 years of age and were eligible to participate. There were 227 males and 252 females. The mean systolic blood pressure was found to rise with age, peaking in the 6l·70 years age group, For women the mean blood pressure rose earlier from the age group of 4-l· 50 years. Mean blood pressures rose with increasing body mass index. lt also varied with occupation and education. The retired and unemployed had a higher blood pressure than those employed and there was an inverse relationship with increasing education. The prevalence of hypertension was 33.6%. More females were hypertensive (36.5%) as compared to males (3 0.4%) and this finding was the same for both systolic and diastolic hypertension. Majority (71.4%) of the hypertensives were undiagnosed. 72.5% of hypertensives who were on treatment were not under control. Hypertension was more prevalent among retirees and illiterates. Prevalence of hypertension increased correspondingly with age. Obesity was associated with hypertension. There was no association with family history of hypertension. Multiple logistic regression showed a positive association only for obesity. ln conclusion, given the high prevalence of hypertension at
    present, it appears that the prevalence will increase as each age cohort grows older. Obesity, especially among housewives is a significant assorted factor.
    Matched MeSH terms: Blood Pressure; Blood Pressure Determination
  17. Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants
    Miean KH, Mohamed S
    J Agric Food Chem, 2001 Jun;49(6):3106-12.
    PMID: 11410016
    Studies were conducted on the flavonoids (myricetin, quercetin, kaempferol, luteolin, and apigenin) contents of 62 edible tropical plants. The highest total flavonoids content was in onion leaves (1497.5 mg/kg quercetin, 391.0 mg/kg luteolin, and 832.0 mg/kg kaempferol), followed by Semambu leaves (2041.0 mg/kg), bird chili (1663.0 mg/kg), black tea (1491.0 mg/kg), papaya shoots (1264.0 mg/kg), and guava (1128.5 mg/kg). The major flavonoid in these plant extracts is quercetin, followed by myricetin and kaempferol. Luteolin could be detected only in broccoli (74.5 mg/kg dry weight), green chili (33.0 mg/kg), bird chili (1035.0 mg/kg), onion leaves (391.0 mg/kg), belimbi fruit (202.0 mg/kg), belimbi leaves (464.5 mg/kg), French bean (11.0 mg/kg), carrot (37.5 mg/kg), white radish (9.0 mg/kg), local celery (80.5 mg/kg), limau purut leaves (30.5 mg/kg), and dried asam gelugur (107.5 mg/kg). Apigenin was found only in Chinese cabbage (187.0 mg/kg), bell pepper (272.0 mg/kg), garlic (217.0 mg/kg), belimbi fruit (458.0 mg/kg), French peas (176.0 mg/kg), snake gourd (42.4 mg/kg), guava (579.0 mg/kg), wolfberry leaves (547.0 mg/kg), local celery (338.5 mg/kg), daun turi (39.5 mg/kg), and kadok (34.5 mg/kg). In vegetables, quercetin glycosides predominate, but glycosides of kaempferol, luteolin, and apigenin are also present. Fruits contain almost exclusively quercetin glycosides, whereas kaempferol and myricetin glycosides are found only in trace quantities.
    Matched MeSH terms: Chromatography, High Pressure Liquid/methods
  18. Early versus delayed initiation of continuous distending pressure for respiratory distress syndrome in preterm infants
    Ho JJ, Henderson-Smart DJ, Davis PG
    Cochrane Database Syst Rev, 2002.
    PMID: 12076463
    The application of a continuous distending pressure (CDP) has been shown to have some benefits in the treatment of pre-term infants with respiratory distress syndrome (RDS). CDP has the potential to reduce lung damage, particularly if applied early before atelectasis has occurred. Early application of CDP may better conserve an infant's own surfactant stores and consequently be more effective than CDP applied later in the course of RDS.
    Matched MeSH terms: Positive-Pressure Respiration/methods*
  19. Simple high-performance liquid chromatographic method for the determination of tocotrienols in human plasma
    Yap SP, Julianto T, Wong JW, Yuen KH
    J Chromatogr B Biomed Sci Appl, 1999 Dec 10;735(2):279-83.
    PMID: 10670741
    A simple high-performance liquid chromatographic method using fluorescence detection was developed for the determination of vitamin E especially delta-, gamma- and alpha-tocotrienols in human plasma. The method entailed direct injection of plasma sample after deproteinization using a 3:2 mixture of acetonitrile-tetrahydrofuran. The mobile phase comprised 0.5% (v/v) of distilled water in methanol. Analyses were run at a flow-rate of 1.5 ml/min with the detector operating at an excitation wavelength of 296 nm and emission wavelength of 330 nm. This method is specific and sensitive, with a quantification limit of approximately 40, 34 and 16 ng/ml for alpha-, gamma- and delta-tocotrienol, respectively. The mean absolute recovery values were about 98% while the within-day and between-day relative standard deviation and percent error values of the assay method were all less than 12.0% for alpha-, gamma- and delta-tocotrienol. The calibration curve was linear over a concentration range of 40-2500, 30-4000 and 16-1000 ng/ml for alpha-, gamma- and delta-tocotrienol, respectively. Application of the method in a bioavailability study for determination of the above compounds was also demonstrated.
    Matched MeSH terms: Chromatography, High Pressure Liquid/methods*
  20. Simple high-performance liquid chromatographic method for determination of alpha-tocopherol in human plasma
    Julianto T, Yuen KH, Noor AM
    J Chromatogr B Biomed Sci Appl, 1999 Sep 10;732(1):227-31.
    PMID: 10517240
    A simple high-performance liquid chromatographic method using UV detection was developed for the determination of alpha-tocopherol in human plasma. The method entailed direct injection of the plasma sample after deproteinization using acetonitrile-tetrahydrofuran (3:2). The mobile phase comprised methanol-tetrahydrofuran (94:6) and analysis was run at a flow-rate of 1.5 ml/min with the detector operating at 292 nm. A Crestpak C18S (5 microm, 250 mm x 4.6 mm ID) was used for the chromatographic separation. The method had a mean recovery of 93%, while the within-day and between-day coefficients of variation and percentage errors were all less than 7%. The speed, specificity, sensitivity and reproducibility of this method make it particularly suitable for routine determination of alpha-tocopherol in human plasma. Moreover, only a small sample plasma volume (100 microl) is required for the analysis.
    Matched MeSH terms: Chromatography, High Pressure Liquid/methods*
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