MyMedR

Fulltext Cardiorespiratory parameters before and after acupuncture: observations in a healthy and diseased state

Mohan V, Das S

Clinics (Sao Paulo), 2012;67(6):673-4.

Comment on: Nascimento RC, Hossri CA, Berwanger O, Carvalho VO. Acupuncture and exercise capacity: a case report. Clinics. 2012;67(2):193–4

Exploring the mechanisms of long COVID: Insights from computational analysis of SARS-CoV-2 gene expression and symptom associations

Das S, Kumar S

J Med Virol, 2023 Sep;95(9):e29077.

PMID: 37675861 DOI: 10.1002/jmv.29077

Long coronavirus disease (COVID) has emerged as a global health issue, affecting a substantial number of people worldwide. However, the underlying mechanisms that contribute to the persistence of symptoms in long COVID remain obscure, impeding the development of effective diagnostic and therapeutic interventions. In this study, we utilized computational methods to examine the gene expression profiles of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and their associations with the wide range of symptoms observed in long COVID patients. Using a comprehensive data set comprising over 255 symptoms affecting multiple organ systems, we identified differentially expressed genes and investigated their functional similarity, leading to the identification of key genes with the potential to serve as biomarkers for long COVID. We identified the participation of hub genes associated with G-protein-coupled receptors (GPCRs), which are essential regulators of T-cell immunity and viral infection responses. Among the identified common genes were CTLA4, PTPN22, KIT, KRAS, NF1, RET, and CTNNB1, which play a crucial role in modulating T-cell immunity via GPCR and contribute to a variety of symptoms, including autoimmunity, cardiovascular disorders, dermatological manifestations, gastrointestinal complications, pulmonary impairments, reproductive and genitourinary dysfunctions, and endocrine abnormalities. GPCRs and associated genes are pivotal in immune regulation and cellular functions, and their dysregulation may contribute to the persistent immune responses, chronic inflammation, and tissue abnormalities observed in long COVID. Targeting GPCRs and their associated pathways could offer promising therapeutic strategies to manage symptoms and improve outcomes for those experiencing long COVID. However, the complex mechanisms underlying the condition require continued study to develop effective treatments. Our study has significant implications for understanding the molecular mechanisms underlying long COVID and for identifying potential therapeutic targets. In addition, we have developed a comprehensive website (https://longcovid.omicstutorials.com/) that provides a curated list of biomarker-identified genes and treatment recommendations for each specific disease, thereby facilitating informed clinical decision-making and improved patient management. Our study contributes to the understanding of this debilitating disease, paving the way for improved diagnostic precision, and individualized therapeutic interventions.

Fulltext Leucocytosis in a case of Lung Cancer: Infection or Paraneoplastic Syndrome? - Dilemma in Diagnosis and Treatment

Pandit S, Choudhury S, Das SK, Nandi S

Med J Malaysia, 2012 Oct;67(5):542-4.

PMID: 23770881

A 65 year old male smoker was diagnosed with squamous cell carcinoma of upper lobe of the right lung complicated with Horner's syndrome and gradually increasing leucocytosis. Alhough the inflammatory biomarker level in serum was low, there was no definite way to determine the cause of the leucocytosis (whether infection or hematologic paraneoplastic syndrome). After empirical antibiotic therapy, his fever subsided but the leucocytosis persisted. It was difficult for us to take a decision regarding the priority of the treatment of infection or the lung cancer. Only after the first cycle chemotherapy, did the leucocytosis rapidly drop down. Normal serum procalcitonin level and quick response to chemotherapy indicated that leucocytosis was a manifestation of paraneoplastic syndrome. Treating the underlying cancer is the first step.

Successful treatment of acute kidney injury secondary to haeme nephropathy in paroxysmal nocturnal haemoglobinuria with alkaline diuresis

Sakthiswary R, Das S, Fadilah SA

EXCLI J, 2012;11:198-203.

PMID: 27298607

Paroxysmal nocturnal haemoglobinuria (PNH) also known as 'Marchiafava Micheli syndrome' is a rare condition which can lead to both acute and chronic forms of renal failure through renal tubular haemosiderin deposition. A 45-year-old lady with underlying PNH, presented with complaints of fever, productive cough followed by dark coloured urine. Investigations revealed pancytopenia with a markedly raised creatinine from her baseline (from 65 mmol/L to 385 mmol/L) consistent with acute kidney injury (AKI). Renal biopsy confirmed the diagnosis of haeme nephropathy. The renal impairment improved rapidly and normalised over a period of 5 days with alkaline diuresis (AD). The patient did not require haemodialysis unlike most other reported cases of AKI secondary to haeme nephropathy in PNH. This is the second reported case of AKI in PNH which was successfully treated with AD alone emphasizing the role of AD as a promising therapeutic strategy in this condition.

Gunshot wound in skeletonised human remains with partial adipocere formation

Mohd Nor F, Das S

J Forensic Leg Med, 2012 Jan;19(1):42-5.

PMID: 22152448 DOI: 10.1016/j.jflm.2011.07.008

We present a case of skeletonised human remains. In the present case report, a body was exhumed from the ground above a cemetery. On exhumation, the body was partially-skeletonised with adipocere formation on the upper part of the body. Autopsy of the body showed two bullets in the right thigh muscle and lumbar vertebrae between L4 and L5. Postmortem changes and destruction of soft tissue made it impossible to determine direction of fire through the body, even in a careful complete autopsy.

Ureteritis cystica: an interesting case with diagnostic dilemma

Zamri Z, Harunarashid, Das S, Ramzisham AR

Clin Ter, 2010;161(5):457-8.

PMID: 20964103

Ureteritis cystica is a rare, benign, proliferative condition. We report the case of a 51-year-old female who complained of dysuria and frequency for the last 10 years. The symptoms, however, increased in severity and frequency over the past one year. Urine culture and sensitivity showed presence of Escherichia Coli which was sensitive to augmentin and ciproflaxocin. The urinary tract ultrasonography and intravenous urography revealed bladder diverticula with multiple small, smooth well defects with sharp borders that protruded into the lumen along the proximal and mid left ureter. This finding was later confirmed by retrograde pyelogram. She was treated and currently is on long term antibiotic therapy. The diagnostic features and management of ureteritis cystic is being discussed in detail.

An interesting case of autonomic dysreflexia

Ohnmar H, Das S, Naicker AS

Clin Ter, 2009;160(5):371-3.

PMID: 19997683

Autonomic dysreflexia (AD) is not an uncommon clinical condition and it is usually detected in patients with complete spinal injuries at or above thoracic 6th vertebral level (T 6). This condition is reported to occur in 48- 60% of cases of spinal cord injury at or above the level of T6. But AD due to injury below T6 is rare. The basic mechanism is thought to be due to excessive, uncontrolled activation of sympathetic system. In the present case, we discuss a persistent AD in 55-yr-old tetraplegic patient with C5 American Spinal Injury Association (ASIA) grade A lesion due to a fall from 10 metre height. MRI examination showed C5 and C6 bi-facets fracture and dislocation with canal compromise. Wiring and fusion was performed but recurrent mucous plugging and aspiration pneumonia and urinary tract infection happened during the hospital stay. Three months later, he was re-admitted with multiple pressure sores, pneumonia, sepsis and high blood pressure. He was administered with nifedepine but the blood pressure kept fluctuating. The present study highlights how the precipitating factors like concomitant urinary tract infection, decubitus ulcers, spasticity triggered the AD attack. The knowledge of the AD and its proper diagnosis and management may be beneficial to all clinicians and the present article attempts to highlight such.

Preconception counseling in the prevention of fetal alcohol syndrome: a unique window of opportunity

Das S, Rao V, Chaudhuri JD

Clin Ter, 2009;160(4):315-22.

PMID: 19795087

The role of alcohol in the causation of Fetal Alcohol Syndrome (FAS) has been firmly established. In most cases events leading to its occurrence have been set into motion prior to conception. Alcohol consumption during pregnancy, the sole causative agent of FAS, can therefore be prevented even prior to conception by implementation of preconception counseling (PCC). The present review examines the important issues involved in the implementation of PCC for the prevention of FAS which can be of immense help to the affected population.

An insight into industrial accidents involving the hand

Abdullah S, Jaafar JM, Das S, Sapuan J

Clin Ter, 2009;160(6):427-33.

PMID: 20198282

BACKGROUND: Industrial hand injuries are a common occurrence and contributes to a large segment of workers' morbidity. The main aim of this study was to look into the day pattern, types and location of upper limbs injures, identify the types of machines involved and highlight the treatment received.
MATERIALS AND METHODS: The present study was performed on 57 industrial workers admitted and treated by the Orthopaedic team of Universiti Kebangsaan Malaysia Medical Centre (UKMMC) for acute injuries sustained whilst working.
RESULTS: The majority of the workers were male (93%). The commonest age group involved was between 25-35 years (48%). The right hand was injured more then the left with majority of injuries involving the fingers. Interestingly, we also observed that injuries occurred more on Saturdays. Laceration was the most common injury followed by fractures and crush injuries. Treatment depends on the type and severity of injuries. For most lacerated wounds, treatment was debridement, primary suturing and antibiotic therapy. Injuries on Saturdays indicated that workers needed a longer time break especially during weekends after a long hectic week.
CONCLUSIONS: Injuries sustained by workers may be prevented by proper working techniques and conditions together with wearing of protective gadgets.

Study site: Pusat Perubatan Universiti Kebangsaan Malaysia (PPUKM)

Test of Sex Estimation Equations on Carpal Bones in a Northeastern Thai Population

Laowatthanaphong S, Das S, Phatsara M, Tuamsuk P, Mahakkanukrauh P

Clin Ter, 2016 Mar-Apr;167(2):29-32.

PMID: 27212569 DOI: 10.7417/CT.2016.1917

Sex assessment is an essential step in person identification, both in forensic and anthropological contexts. Many parts of skeletal remains such as skull, pelvis and long bones have been proven to be useful in determining sex. However, literature has shown that short bones such as carpal bones are also sexually dimorphic. In the last few years, there was an unpublished study using lunate, scaphoid and hamate from bone collection in Northern Thailand to create 6 discriminant equations to assess sex. The objective of this study was to investigate the application of those equations in the sample from other parts of Thailand.

An insight into the harmful effects of soy protein: A review

Sukalingam K, Ganesan K, Das S, Thent ZC

Clin Ter, 2015;166(3):131-9.

PMID: 26152621 DOI: 10.7417/CT.2015.1843

Soy protein (SP) is a protein derived from soybean meal. SP is obtained from the removal of the outer shell of soybean and the fatty acid. The dietary supplementation of SP was was reported to have positive effects on human health. Therefore, the attention towards SP is increasing among the consumers, industrialist and researchers. However, the side effects and toxicity related to SP was not summarized, to date. This review summarized the toxic effects such as hormonal disturbances, carcinogenic and organotoxicity of SP based on the clinical and experimental studies. The review mainly focused on the effect of soy isoflavone-genistein on various organs. The main aim of the present review is to increase the public awareness on the harmful effect of SP on the various health aspects and draw the attention of the health care personnel and researchers.

Fulltext Blood-brain barrier dysfunction in ischemic stroke and diabetes: the underlying link, mechanisms and future possible therapeutic targets

Wicha P, Das S, Mahakkanukrauh P

Anat Cell Biol, 2021 Jun 30;54(2):165-177.

PMID: 33658432 DOI: 10.5115/acb.20.290

Ischemic stroke caused by occlusion of cerebral artery is responsible for the majority of stroke that increases the morbidity and mortality worldwide. Diabetes mellitus (DM) is a crucial risk factor for ischemic stroke. Prolonged DM causes various microvascular and macrovascular changes, and blood-brain barrier (BBB) permeability that facilitates inflammatory response following stroke. In the acute phase following stroke, BBB disruption has been considered the initial step that induces neurological deficit and functional disabilities. Stroke outcomes are significantly worse among DM. In this article, we review stroke with diabetes-induce BBB damage, as well as underlying mechanism and possible therapeutic targets for stroke with diabetes.

Fulltext Placental histopathological changes in COVID-19 in term patients admitted to labor and delivery

Ahmad MFB, Das S

Am J Obstet Gynecol, 2021 09;225(3):353-354.

PMID: 34062121 DOI: 10.1016/j.ajog.2021.05.040

Fulltext Sex estimation by femur in modern Thai population

Monum T, Prasitwattanseree S, Das S, Siriphimolwat P, Mahakkanukrauh P

Clin Ter, 2017 6 15;168(3):e203-e207.

PMID: 28612898 DOI: 10.7417/T.2017.2007

Sex estimation is an important step of postmortem investigation and the femur is a useful bone for sex estimation by using metric analysis method. Even though there have been a reported sex estimation method by using femur in Thais, the temporal change related to time and anthropological data need to be renewed. Thus the aim of this study is to re-evaluate sex estimation by femur in Thais. 97 adult male and 103 female femora were random chosen from Forensic osteology research center and 6 measurements were applied tend to. To compare with previous Thai data, mid shaft diameter to increase but femoral head and epicondylar breadth to stabilize and when tested previous discriminant function by vertical head diameter and epicondalar breadth, the accuracy of prediction was lower than previous report. From the new data, epicondalar breadth is the best variable for distinguishing male and female at 88.7 percent of accuracy, following by transverse and vertical head diameter at 86.7 percent and femoral neck diameter at 81.7 percent of accuracy. Multivariate discriminant analysis indicated transverse head diameter and epicondylar breadth performed highest rate of accuracy at 89.7 percent. The percent of accuracy of femur was close to previous reported sex estimation by talus and calcaneus in Thai population. Thus, for especially in case of lower limb remain, which absence of pelvis.

The Accuracy of Sex Estimation on Metatarsal Bones in a Northeastern Thai Population

Phatsara M, Das S, Laowatthanaphong S, Tuamsuk P, Mahakkanukrauh P

Clin Ter, 2016 May-Jun;167(3):72-6.

PMID: 27424506 DOI: 10.7417/CT.2016.1929

BACKGROUND: This study was carried out to evaluate the accuracy of sex estimation by discriminant analysis and stepwise discriminant analysis equations generated from metatarsal bones in a Thai population.
MATERIAL AND METHODS: The testing samples utilized in this study consisted of 50 skeletons (25 males and 25 females) obtained from the Khon Kaen University Skeletal Collection, Department of Anatomy, Faculty of Medicine, Khon Kaen University. Seven measurements of metatarsal bones were measured in centimeters, using either a mini-osteometric board (MOB) or a sliding caliper. The values measured from the Khon Kaen Skeletal Collection were used to determine the accuracy and applicability of sex determination, as predicted by Y1-Y6 equations which were generated from a Chiang Mai Skeletal Collection.
RESULTS: The percentage of sex determination accuracies predicted from the Y1-Y6 equations demonstrated accuracy rates of 80-95.6.
CONCLUSIONS: The Chiang Mai sex determination equations, generated from metatarsal bones by discriminant analysis (Y1-Y3) and stepwise discriminant analysis (Y4-Y6), demonstrated high accuracy rates of prediction, suggesting that these equations may be useful for sex determination within the Thai population.
KEYWORDS: Foot; Metatarsal bones; Sex determination; Thailand

Fulltext Morphology of the human aorta and age-related changes: anatomical facts

Komutrattananont P, Mahakkanukrauh P, Das S

Anat Cell Biol, 2019 Jun;52(2):109-114.

PMID: 31338225 DOI: 10.5115/acb.2019.52.2.109

Aorta is the largest artery in the human body. Its starting point is the aortic orifice of the aortic valve and it terminates at the level of the fourth lumbar vertebra. The main function of the aorta is to transport oxygenated blood to supply all the organs and cells. With advancing age, the structure and hence the function show progressive changes. Various changes in the aortic morphology include the luminal diameter of aorta, whole length of the aorta, thickness, the microstructural components also change, and these include collagen, elastin and smooth muscle cells. In addition, the dimensions of all segments of the aorta increase with age in both sexes. Since age is a major risk factor for degenerative change and diseases affecting the aorta, understanding the detailed anatomy of the aorta may provide essential information concerning the age-associated process of the aorta. Knowledge of the morphological changes in the aorta is also important for future clinical therapies pertaining to aortic disease. Additionally, the information regarding the structural changes with age may be applied for age determination. This review describes the overview of the anatomy of the aorta, age related changes in the morphology of the aorta and aortic diseases.

Fulltext Cocktail Protocol for Preparation of Platelet-Rich Fibrin Glue for Autologous Use

Chenna D, Shastry S, Das S

Malays J Med Sci, 2021 Feb;28(1):35-40.

PMID: 33679218 DOI: 10.21315/mjms2021.28.1.5

Background: Biomaterials containing platelets have been used to promote healing of ulcers and burns, as well as in implantology and maxillofacial and plastic surgery to achieve wound healing and tissue repair. Commercial devices to prepare autologous biomaterials involve diverse preparation methods that can have high production costs and low yields. Hence, we designed a protocol for preparation of large amounts of autologous platelet-rich fibrin (PRF) glue using conventional processing techniques for blood components.

Methods: Autologous whole blood collected 72 h before surgery was processed to prepare platelet concentrates and cryoprecipitate. In a closed system, calcium was added to the cryoprecipitate to release autologous thrombin and generate a firm fibrin clot. The fibrin clot, platelets and calcium were then placed in a conical flask in which a PRF glue formed. The protocol was validated through determination of pre- and post-platelet counts and fibrinogen amounts in the product.

Results: Platelets were recovered with 68% efficiency during the preparation. Essentially no platelets or fibrinogen were found in the supernatant of the PRF glue, suggesting that nearly all had been incorporated in a PRF glue having a relatively large (8 cm × 10 cm) size.

Conclusion: The protocol described here is a cost-effective, simple and closed system that can be used to produce large-size PRF glue to promote repair of major surgical defects.

Fulltext Vienna nomogram-based prostate biopsy: can it be a much better diagnostic tool than other conventional prostate biopsies?

Ho CC, Ho SE, Das S

Korean J Urol, 2014 Sep;55(9):628.

PMID: 25237467 DOI: 10.4111/kju.2014.55.9.628

Re: Giorgio Gandaglia, Alberto Briganti, Graham Jackson, et Al. A systematic review of the association between erectile dysfunction and cardiovascular disease. Eur urol 2014;65:968-78

Ho CC, Ho SE, Das S

Eur Urol, 2014 Nov;66(5):e87.

PMID: 24951360 DOI: 10.1016/j.eururo.2014.06.003

Clinical importance of duplicated internal jugular vein

Das S, Ho CK, Eng HS

Br J Oral Maxillofac Surg, 2014 Oct;52(8):773.

PMID: 24947333 DOI: 10.1016/j.bjoms.2014.05.015

Displaying publications 41 - 60 of 559 in total