Displaying publications 41 - 60 of 80 in total

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  1. The contraceptive role of breastfeeding by educational attainment: an assessment based on Malaysian fertility and family survey
    Othman A
    Malays J Reprod Health, 1985 Jun;3(1):77-83.
    PMID: 12314429
  2. Sequence analysis and gene expression of putative oil palm chitinase and chitinase-like proteins in response to colonization of Ganoderma boninense and Trichoderma harzianum
    Yeoh KA, Othman A, Meon S, Abdullah F, Ho CL
    Mol Biol Rep, 2013 Jan;40(1):147-58.
    PMID: 23065213 DOI: 10.1007/s11033-012-2043-8
    Chitinases are glycosyl hydrolases that cleave the β-1,4-glycosidic linkages between N-acetylglucosamine residues in chitin which is a major component of fungal cell wall. Plant chitinases hydrolyze fungal chitin to chitin oligosaccharides that serve as elicitors of plant defense system against fungal pathogens. However, plants synthesize many chitinase isozymes and some of them are not pathogenesis-related. In this study, three full-length cDNA sequences encoding a putative chitinase (EgChit3-1) and two chitinase-like proteins (EgChit1-1 and EgChit5-1) have been cloned from oil palm (Elaeis guineensis) by polymerase chain reaction (PCR). The abundance of these transcripts in the roots and leaves of oil palm seedlings treated with Ganoderma boninense (a fungal pathogen) or Trichoderma harzianum (an avirulent symbiont), and a combination of both fungi at 3, 6 and 12 weeks post infection were profiled by real time quantitative reverse-transcription (qRT)-PCR. Our findings showed that the gene expression of EgChit3-1 increased significantly in the roots of oil palm seedlings treated with either G. boninense or T. harzianum and a combination of both; whereas the gene expression of EgChit1-1 in the treated roots of oil palm seedlings was not significantly higher compared to those of the untreated oil palm roots. The gene expression of EgChit5-1 was only higher in the roots of oil palm seedlings treated with T. harzianum compared to those of the untreated oil palm roots. In addition, the gene expression of EgChit1-1 and EgChit3-1 showed a significantly higher gene expression in the leaf samples of oil palm seedlings treated with either G. boninense or T. harzianum.
  3. Ex vivo expanded SSEA-4+ human limbal stromal cells are multipotent and do not express other embryonic stem cell markers
    Lim MN, Hussin NH, Othman A, Umapathy T, Baharuddin P, Jamal R, et al.
    Mol Vis, 2012;18:1289-300.
    PMID: 22665977
    The presence of multipotent human limbal stromal cells resembling mesenchymal stromal cells (MSC) provides new insights to the characteristic of these cells and its therapeutic potential. However, little is known about the expression of stage-specific embryonic antigen 4 (SSEA-4) and the embryonic stem cell (ESC)-like properties of these cells. We studied the expression of SSEA-4 surface protein and the various ESC and MSC markers in the ex vivo cultured limbal stromal cells. The phenotypes and multipotent differentiation potential of these cells were also evaluated.
  4. Fulltext Sustainable Palm Oil-The Role of Screening and Advanced Analytical Techniques for Geographical Traceability and Authenticity Verification
    Ramli US, Tahir NI, Rozali NL, Othman A, Muhammad NH, Muhammad SA, et al.
    Molecules, 2020 Jun 25;25(12).
    PMID: 32630515 DOI: 10.3390/molecules25122927
    Palm oil production from oil palm (Elaeis guineensis Jacq.) is vital for the economy of Malaysia. As of late, sustainable production of palm oil has been a key focus due to demand by consumer groups, and important progress has been made in establishing standards that promote good agricultural practices that minimize impact on the environment. In line with the industrial goal to build a traceable supply chain, several measures have been implemented to ensure that traceability can be monitored. Although the palm oil supply chain can be highly complex, and achieving full traceability is not an easy task, the industry has to be proactive in developing improved systems that support the existing methods, which rely on recorded information in the supply chain. The Malaysian Palm Oil Board (MPOB) as the custodian of the palm oil industry in Malaysia has taken the initiative to assess and develop technologies that can ensure authenticity and traceability of palm oil in the major supply chains from the point of harvesting all the way to key downstream applications. This review describes the underlying framework related to palm oil geographical traceability using various state-of-the-art analytical techniques, which are also being explored to address adulteration in the global palm oil supply chain.
  5. Homozygous variant of UGT1A1 gene mutation and severe neonatal hyperbilirubinemia
    Boo NY, Wong FL, Wang MK, Othman A
    Pediatr Int, 2009 Aug;51(4):488-93.
    PMID: 19674361 DOI: 10.1111/j.1442-200X.2008.02798.x
    The aim of the present study was to compare, in a case-control study, the prevalence of nucleotide 211 guanine to adenine (G-->A) mutation of uridine diphosphoglucuronosyl transferase (UGT1A1) gene in Malaysian Chinese newborns with and without severe hyperbilirubinemia (total serum bilirubin >250 micromol/L during first 48 h of life or > or =300 micromol/L thereafter), and to determine whether this mutation was a significant risk factor associated with severe hyperbilirubinemia.
  6. GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene
    Hanifiah FHA, Abdullah SNA, Othman A, Shaharuddin NA, Saud HM, Hasnulhadi HAH, et al.
    Plant Cell Rep, 2018 Aug;37(8):1127-1143.
    PMID: 29789886 DOI: 10.1007/s00299-018-2300-y
    KEY MESSAGE: TAAAAT and a novel motif, GCTTCA found in the oil palm stearoyl-ACP desaturase (SAD1) promoter are involved in regulating mesocarp-specific expression. Two key fatty acid biosynthetic genes, stearoyl-ACP desaturase (SAD1), and acyl-carrier protein (ACP3) in Elaeis guineensis (oil palm) showed high level of expression during the period of oil synthesis in the mesocarp [12-19 weeks after anthesis (w.a.a.)] and kernel (12-15 w.a.a.). Both genes are expressed in spear leaves at much lower levels and the expression increased by 1.5-fold to 2.5-fold following treatments with ethylene and abscisic acid (ABA). Both SAD1 and ACP3 promoters contain phytohormone-responsive, light-responsive, abiotic factors/wounding-responsive, endosperm specificity and fruit maturation/ripening regulatory motifs. The activities of the full length and six 5' deletion fragments of the SAD1 promoter were analyzed in transiently transformed oil palm tissues by quantitative β-glucuronidase (GUS) fluorometric assay. The highest SAD1 promoter activity was observed in the mesocarp followed by kernel and the least in the leaves. GUS activity in the D3 deletion construct (- 486 to + 108) was the highest, while the D2 (- 535 to + 108) gave the lowest suggesting the presence of negative cis-acting regulatory element(s) in the deleted - 535 to - 486 (49 bp). It was found that the 49-bp region binds to the nuclear protein extract from mesocarp but not from leaves in electrophoretic mobility shift assay (EMSA). Further fine-tuned analysis of this 49-bp region using truncated DNA led to the identification of GCTTCA as a novel motif in the SAD1 promoter. Interestingly, another known fruit ripening-related motif, LECPLEACS2 (TAAAAT) was found to be required for effective binding of the novel motif to the mesocarp nuclear protein extract.
  7. Fulltext Intracellular Delivery of Proteins via Fusion Peptides in Intact Plants
    Ng KK, Motoda Y, Watanabe S, Sofiman Othman A, Kigawa T, Kodama Y, et al.
    PLoS One, 2016;11(4):e0154081.
    PMID: 27100681 DOI: 10.1371/journal.pone.0154081
    In current plant biotechnology, the introduction of exogenous DNA encoding desired traits is the most common approach used to modify plants. However, general plant transformation methods can cause random integration of exogenous DNA into the plant genome. To avoid these events, alternative methods, such as a direct protein delivery system, are needed to modify the plant. Although there have been reports of the delivery of proteins into cultured plant cells, there are currently no methods for the direct delivery of proteins into intact plants, owing to their hierarchical structures. Here, we demonstrate the efficient fusion-peptide-based delivery of proteins into intact Arabidopsis thaliana. Bovine serum albumin (BSA, 66 kDa) was selected as a model protein to optimize conditions for delivery into the cytosol. The general applicability of our method to large protein cargo was also demonstrated by the delivery of alcohol dehydrogenase (ADH, 150 kDa) into the cytosol. The compatibility of the fusion peptide system with the delivery of proteins to specific cellular organelles was also demonstrated using the fluorescent protein Citrine (27 kDa) conjugated to either a nuclear localization signal (NLS) or a peroxisomal targeting signal (PTS). In conclusion, our designed fusion peptide system can deliver proteins with a wide range of molecular weights (27 to 150 kDa) into the cells of intact A. thaliana without interfering with the organelle-targeting peptide conjugated to the protein. We expect that this efficient protein delivery system will be a powerful tool in plant biotechnology.
  8. Fulltext Evaluation of sodium deoxycholate as solubilization buffer for oil palm proteomics analysis
    Lau BYC, Othman A
    PLoS One, 2019;14(8):e0221052.
    PMID: 31415606 DOI: 10.1371/journal.pone.0221052
    Protein solubility is a critical prerequisite to any proteomics analysis. Combination of urea/thiourea and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) have been routinely used to enhance protein solubilization for oil palm proteomics studies in recent years. The goals of these proteomics analysis are essentially to complement the knowledge regarding the regulation networks and mechanisms of the oil palm fatty acid biosynthesis. Through omics integration, the information is able to build a regulatory model to support efforts in improving the economic value and sustainability of palm oil in the global oil and vegetable market. Our study evaluated the utilization of sodium deoxycholate as an alternative solubilization buffer/additive to urea/thiourea and CHAPS. Efficiency of urea/thiourea/CHAPS, urea/CHAPS, urea/sodium deoxycholate and sodium deoxycholate buffers in solubilizing the oil palm (Elaeis guineensis var. Tenera) mesocarp proteins were compared. Based on the protein yields and electrophoretic profile, combination of urea/thiourea/CHAPS were shown to remain a better solubilization buffer and additive, but the differences with sodium deoxycholate buffer was insignificant. A deeper mass spectrometric and statistical analyses on the identified proteins and peptides from all the evaluated solubilization buffers revealed that sodium deoxycholate had increased the number of identified proteins from oil palm mesocarps, enriched their gene ontologies and reduced the number of carbamylated lysine residues by more than 67.0%, compared to urea/thiourea/CHAPS buffer. Although only 62.0% of the total identified proteins were shared between the urea/thiourea/CHAPS and sodium deoxycholate buffers, the importance of the remaining 38.0% proteins depends on the applications. The only observed limitations to the application of sodium deoxycholate in protein solubilization were the interference with protein quantitation and but it could be easily rectified through a 4-fold dilution. All the proteomics data are available via ProteomeXchange with identifier PXD013255. In conclusion, sodium deoxycholate is applicable in the solubilization of proteins extracted from oil palm mesocarps with higher efficiency compared to urea/thiourea/CHAPS buffer. The sodium deoxycholate buffer is more favorable for proteomics analysis due to its proven advantages over urea/thiourea/CHAPS buffer.
  9. Unravelling the temporal association between lameness and body condition score in dairy cattle using a multistate modelling approach
    Lim PY, Huxley JN, Willshire JA, Green MJ, Othman AR, Kaler J
    Prev Vet Med, 2015 Mar 1;118(4):370-7.
    PMID: 25579605 DOI: 10.1016/j.prevetmed.2014.12.015
    Recent studies have reported associations between lameness and body condition score (BCS) in dairy cattle, however the impact of change in the dynamics of BCS on both lameness occurrence and recovery is currently unknown. The aim of this longitudinal study was to investigate the effect of change in BCS on the transitions from the non-lame to lame, and lame to non-lame states. A total of 731 cows with 6889 observations from 4 UK herds were included in the study. Mobility score (MS) and body condition score (BCS) were recorded every 13-15 days from July 2010 until December 2011. A multilevel multistate discrete time event history model was built to investigate the transition of lameness over time. There were 1042 non-lame episodes and 593 lame episodes of which 50% (519/1042) of the non-lame episodes transitioned to the lame state and 81% (483/593) of the lame episodes ended with a transition to the non-lame state. Cows with a lower BCS at calving (BCS Group 1 (1.00-1.75) and Group 2 (2.00-2.25)) had a higher probability of transition from non-lame to lame and a lower probability of transition from lame to non-lame compared to cows with BCS 2.50-2.75, i.e. they were more likely to become lame and if lame, they were less likely to recover. Similarly, cows who suffered a greater decrease in BCS (compared to their BCS at calving) had a higher probability of becoming lame and a lower probability of recovering in the next 15 days. An increase in BCS from calving was associated with the converse effect, i.e. a lower probability of cows moving from the non-lame to the lame state and higher probability of transition from lame to non-lame. Days in lactation, quarters of calving and parity were associated with both lame and non-lame transitions and there was evidence of heterogeneity among cows in lameness occurrence and recovery. This study suggests loss of BCS and increase of BCS could influence the risk of becoming lame and the chance of recovery from lameness. Regular monitoring and maintenance of BCS on farms could be a key tool for reducing lameness. Further work is urgently needed in this area to allow a better understanding of the underlying mechanisms behind these relationships.
  10. Piloting a psycho-education program for parents of pediatric cancer patients in Malaysia
    Othman A, Blunden S, Mohamad N, Mohd Hussin ZA, Jamil Osman Z
    Psychooncology, 2010 Mar;19(3):326-31.
    PMID: 19462470 DOI: 10.1002/pon.1584
    To evaluate a psycho-educational program (PeP) for parents of children with cancer (PoCwC) in Malaysia.
  11. Impact of shoreline changes to pahang coastal area by using geospatial technology
    Fazly Amri Mohd, Khairul Nizam Abdul Maulud, Othman A. Karim, Rawshan Ara Begum, Siti Norsakinah Selamat
    Sains Malaysiana, 2018;47:991-997.
    Malaysia has a long coastline stretching over 4,809 km where more than 1,300 km of beaches are experiencing erosion.
    Coastal erosion is recognised as the permanent loss of land and habitats along the shoreline resulting in the changes
    of the coast. Thus, it is important to detect and monitor shoreline changes especially in Pahang coast by identifying the
    rate of shoreline erosion and accretion. This study used temporal data and high spatial resolution imagery (SPOT 5) using
    remote sensing and GIS techniques to monitor shoreline changes along 10 study locations, which is from Cherating to
    Pekan of the Pahang coast. The total length of shoreline changes is about 14 km (14035.10 m) where all these areas are
    very likely to experience erosion ranging from 0.1 to 94.7 ha. On the other hand, these coastal areas found a minimal
    accretion with increased sediment from 0.1 to 2.8 ha. Overall, the coastal areas are exposed to higher erosion process
    than accretion with a very high vulnerability of erosion rate from 1.8 to 20.9 meter per year. The findings on monitoring
    shoreline changes and identifying vulnerable erosion areas might be useful in the policy and decision making for
    sustainable coastal management.
  12. Impak perubahan datum carta dan kecerunan pesisir depan terhadap ketidakpastian kedudukan titik pangkal Malaysia
    Zaaim Hasan, Othman A. Karim, Khairul Nizam Abdul Maulud
    Sains Malaysiana, 2008;37.
    Malaysia mempunyai tanggungjawab mendepositkan koordinat-koordinat titik-titik pangkal yang digunakan untuk mengukur lebar batas laut wilayah dan sempadan zon-zon maritim yang lain kepada Setiausaha Agung, Pertubuhan Bangsa-Bangsa Bersatu (PBB). Titik-titik pangkal adalah asas kepada penetapan bagi zon-zon maritim, perundangan batas maritim dan perlaksanaan penguatkuasaan. Kertas ini menghuraikan bagaimana dua parameter penting iaitu kecerunan pesisir depan dan datum carta boleh menyebabkan ketidakpastian terhadap posisi titik-titik pangkal. Hubungkait trigonometri antara parameter membuktikan sisihan datum sebanyak 0.2 m dan kecerunan pesisir depan sebanyak 0.1º menyebabkan ketidakpastian posisi mendatar sebanyak 114.6 m. Ketidakpastian kedudukan titik pangkal menjadi signifikan apabila sisihan antara datum carta meningkat dan kecerunan pesisir depan semakin landai. Hasil prosesan kedudukan titik-titik pangkal melalui perisian “CARIS” mendapati perbezaan kedudukan titik-titik pangkal sebanyak 108 m, 169 m dan 107 m. Kesimpulannya, ketidakpastian ini boleh menyebabkan negara kerugian kawasan maritim.
  13. Study of Cohesive Sediment using Ultrasound
    Othman A. Karim, Crapper M, Ali K.H.M.
    Sains Malaysiana, 1997;26.
    The study of cohesive sediment in the laboratory gives rise to a number of instrumentation problems, especially in the location of mud bed, fluid mud and hindered settling layers and in the measurement of flow velocities. This paper describes the application of medical diagnostic ultrasound technique in the cohesive sediment study conducted at the University of Liverpool, United Kingdom. This paper illustrates that the use of ultrasound technique creates a reasonably flexible environment for the study of fluid mud phenomenon in which bed formation and flow velocities can be measured easily, accurately and non-intrusively. This in turn will assist in development of computer models to predict the environmental impact, siltation rates and dredging requirements in both new and existing ports and harbour developments.
    Kajian endapan berjeleket di dalam makmal mengalami pelbagai masalah peralatan, terutamanya bagi menentukan lokasi dasar lumpur, pengenapan terhalang dan pengukuran halaju aliran. Dalam makalah ini diterangkan penggunaan teknologi diagnosis perubatan ultrabunyi dalam kajian endapan berjeleket, yang dijalankan di University of Liverpool, United Kingdom. Ditunjukkan bahawa penggunaan teknologi ultrabunyi keadaan yang begitu boleh suai bagi kajian fenomenon lumpur yang pembentukan dasar dan halaju aliran dapat diukur dengan mudah, tepat dan tanpa gangguan. lni seterusnya dapat membantu di dalam pembangunan model komputer bagi menjangka kesan sekitaran, kadar enapan dan keperluan mengorek bagi pembangunan kawasan pelabuhan baru dan sedia ada.
  14. Kajian kualitatif perubahan guna tanah kawasan bandar Pekanbaru dengan menggunakan sistem maklumat geografi (Gis)
    Yusri, Othman A. Karim, Khairul Nizam Abdul Maulud, Mohd. Ekhwan Toriman
    Sains Malaysiana, 2010;39.
    Penggunaan tanah boleh mengakibatkan perubahan fungsi dari masa ke masa, sesuai dengan tujuan pembangunan. Kawasan Bandar Pekanbaru terdiri daripada 12 sub-kawasan persekutuan dengan jumlah keluasan kira-kira 632.26 km2. Tujuan kajian adalah untuk menganalisis perubahan guna tanah daripada aspek ruang dan masa. Data guna tanah yang diambil kira dalam analisis ini adalah peta guna tanah 1992 dan 2004. Hasil analisis dengan menggunakan perisian ArcView GIS versi 3.2 mendapati bahawa telah berlaku penurunan guna tanah signifikan, iaitu jenis perkebunan dan hutan tahun 2004. Peningkatan jenis lain-lain, penggunaan tanah seperti permukiman dan tumbuhan/semak tahun 2004 telah berlaku. Perubahan ini merupakan pengaruh daripada pembangunan bandar. Sebaliknya, pembangunan bandar boleh memberi impak negatif terhadap sekitaran jika pola perubahan guna tanah dirancang tanpa pengurusan yang baik.
  15. Immature reticulocyte fraction is an early predictor of bone marrow recovery post chemotherapy in patients with acute leukemia
    Raja-Sabudin RZ, Othman A, Ahmed-Mohamed KA, Ithnin A, Alauddin H, Alias H, et al.
    Saudi Med J, 2014 Apr;35(4):346-9.
    PMID: 24749130
    To establish the benefits of immature reticulocyte fraction (IRF) measurement using an automated hematology cells analyzer over absolute neutrophil count (ANC) in predicting bone marrow recovery post induction chemotherapy.
  16. Immunophenotyping analysis of lymph node biopsies by flow cytometry
    Raja-Sabudin RZ, Hamid AA, Yusof N, Alauddin H, Aziz SA, Kulaveerasingam S, et al.
    Saudi Med J, 2012 Oct;33(10):1131-3.
    PMID: 23047221
  17. Fulltext Increased behavioural problems associated with corticosteroid use in children with nephrotic syndrome: a Southeast Asian perspective
    Mahmud MN, Othman A, Ilias MI
    Singapore Med J, 2023 Sep;64(9):557-562.
    PMID: 34600452 DOI: 10.11622/smedj.2021128
    INTRODUCTION: The study was performed to determine the psychological problems in children with idiopathic nephrotic syndrome (INS) while they were on steroid therapy, as compared to healthy children.

    METHODS: This prospective cohort study was conducted in a paediatric clinic of a tertiary hospital. Parents of the participants in the INS group and control group (comprising children without chronic illness) completed questionnaires using the Child Behavioural Checklist (CBCL). The CBCL measures a range of age-specific emotional and psychological problems, including internalising and externalising domains. Analyses of the CBCL scores between groups were done using Mann-Whitney U test.

    RESULTS: A total of 140 children were recruited with an equal number in the INS and control groups. There was a significant difference in the mean total CBCL scores between the INS group and the control group, specifically in the withdrawal, somatic, anxious and aggressiveness subdomains. Similar findings were demonstrated in correlation between total psychological problems and corticosteroid dosage. In the INS group, steroid dose and cushingoid features were found to have a significant positive association with internalising psychological problems.

    CONCLUSION: Children with INS on corticosteroid treatment showed an increase in internalising and externalising scores, as compared to healthy children.

  18. Barriers to sexual activity: counselling spinal cord injured women in Malaysia
    Julia PE, Othman AS
    Spinal Cord, 2011 Jul;49(7):791-4.
    PMID: 21321578 DOI: 10.1038/sc.2011.4
    This study is a cross-sectional, face-to-face interview.
  19. Induction of labour from 39 weeks in low-risk multiparas with ripe cervixes: A randomised controlled trial
    Tan PC, Othman A, Win ST, Hong JGS, Elias N, Omar SZ
    Aust N Z J Obstet Gynaecol, 2021 Jun 05.
    PMID: 34089525 DOI: 10.1111/ajo.13377
    BACKGROUND: Induction of labour (IOL) in low-risk nulliparas at 39 weeks reduces caesarean delivery. Multiparas with ripe cervixes typically have vaginal delivery within eight hours. Delivery at night and weekend are associated with higher maternal and neonatal mortality.

    AIMS: To evaluate IOL in full-term multiparas with ripe cervixes to achieve delivery at normal working hours and improve maternal satisfaction.

    METHODS: A randomised trial was performed in a tertiary hospital in Malaysia. Low-risk multiparas with ripe cervixes (Bishop score ≥6) were recruited at 38+4 -40+0  weeks, then randomised to planned labour induction at 39+0  weeks or expectant care. Primary outcomes were delivery during 'normal working hours' 09:00-17:00 hours, Monday-Friday and patient satisfaction by visual numerical rating scale.

    RESULTS: For IOL (n = 80) vs expectant care (n = 80) arms respectively, primary outcomes of delivery at normal working hours was 27/80 (34%) vs 29/78 (37%), relative risk (RR) 0.9, 95% CI 0.5-1.7, P = 0.41, patient satisfaction was 8.0 ± 1.8 vs 7.8 ± 1.6, P = 0.41; presentation for spontaneous labour or rupture of membranes were 27/80 (34%) vs 70/79 (89%), RR 0.4, 95% CI 0.3-0.5, P 

  20. Band 3 mutations, renal tubular acidosis and South-East Asian ovalocytosis in Malaysia and Papua New Guinea: loss of up to 95% band 3 transport in red cells
    Bruce LJ, Wrong O, Toye AM, Young MT, Ogle G, Ismail Z, et al.
    Biochem. J., 2000 Aug 15;350 Pt 1:41-51.
    PMID: 10926824
    We describe three mutations of the red-cell anion exchangerband 3 (AE1, SLC4A1) gene associated with distalrenal tubular acidosis (dRTA) in families from Malaysia and Papua NewGuinea: Gly(701)-->Asp (G701D), Ala(858)-->Asp(A858D) and deletion of Val(850) (DeltaV850). The mutationsA858D and DeltaV850 are novel; all three mutations seem to berestricted to South-East Asian populations. South-East Asianovalocytosis (SAO), resulting from the band 3 deletion of residues400-408, occurred in many of the families but did not itselfresult in dRTA. Compound heterozygotes of each of the dRTA mutationswith SAO all had dRTA, evidence of haemolytic anaemia and abnormal red-cell properties. The A858D mutation showed dominant inheritance and therecessive DeltaV850 and G701D mutations showed a pseudo-dominantphenotype when the transport-inactive SAO allele was also present. Red-cell and Xenopus oocyte expression studies showed that theDeltaV850 and A858D mutant proteins have greatly decreased aniontransport when present as compound heterozygotes (DeltaV850/A858D,DeltaV850/SAO or A858D/SAO). Red cells with A858D/SAO had only 3% ofthe SO(4)(2-) efflux of normal cells, thelowest anion transport activity so far reported for human red cells. The results suggest dRTA might arise by a different mechanism for eachmutation. We confirm that the G701D mutant protein has an absoluterequirement for glycophorin A for movement to the cell surface. Wesuggest that the dominant A858D mutant protein is possibly mis-targetedto an inappropriate plasma membrane domain in the renal tubular cell,and that the recessive DeltaV850 mutation might give dRTA because ofits decreased anion transport activity.
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