Displaying publications 41 - 60 of 98 in total

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  1. Nurimatussolehah Sarijan, Sabariah Md Noor, Tun Maizura Mohd Fathullah, Malina Osman, Zainina Seman
    MyJurnal
    Percentage of haemolysis is widely used as a quality parameter to assess red blood cell viability in blood banking. In certain blood banks, serum potassium level is used due to the unavailability of the former test. The relationship between these two tests, however, is still unclear. The objective of this study is to determine the association between haemolysis measured using two different methods for quality control. Methods: A total of forty-four samples of packed red cell in citrate-phosphate-dextrose with optisol were randomly selected from donation drives. Nine millilitres of blood was collected weekly starting from day-2 of storage, followed by day-7, 14, 21, 28, 35 and 42 for assessment of red blood cell haemolysis by measuring serum potassium level and percentage of haemolysis.Results: These two parameters were correlated significantly with a positive moderate linear relationship on day 7, 21 and 28 with r = 0.393, 0.448 and 0.425, respectively and p-values less than 0.01. The linear regression analysis showed there was a significant regression equation which could be used to predict the serum potassium level from the percentage of haemolysis. Conclusion: There were significant increases in the percentage of haemolysis and serum potassium level in the packed red cell units with storage. The serum potassium level would be able to be predicted from the percentage of haemolysis using the regression equations on day 7, 21 and 28. The serum potassium measurement could be used as an alternative test to the percentage of haemolysis before issuing blood.
    Matched MeSH terms: Chondroitin Sulfates
  2. Ahmed Atia, Nadia Salem Alrawaiq, Azman Abdullah
    Sains Malaysiana, 2018;47:2799-2809.
    Glutathione S-transferase isoenzymes (GSTs) catalyze the conjugation reaction between glutathione and electrophilic
    compounds. GSTs are involved in the detoxification of toxic and carcinogenic compounds, thus protecting the body from
    toxic injuries. Tocotrienols are part of the vitamin E family and is believed to possess potent antioxidant activity. The
    objective of this study was to determine the effect of increasing doses of tocotrienol rich fraction (TRF) supplementation
    on liver GSTs gene and protein expression. A total of 30 male ICR white mice were divided into five groups (n=6 for each
    group) and given treatment for 14 days through oral supplementation. Groups were divided as follows: - three groups
    administered with TRF at doses of 200, 500 and 1000 mg/kg, respectively, a positive control group administered with 100
    mg/kg butylated hydroxyanisole (BHA) and a control group administered with only the vehicle (corn oil). At day 15, the
    mice were sacrificed and their livers isolated. Total RNA was extracted from the liver and quantitative real-time polymerase
    chain reaction (qPCR) assays were performed to analyze GSTs gene expression. Total liver protein was also extracted
    and the protein expression of GSTs was determined by Western blotting. The results showed that TRF oral supplementation
    caused a significant dose-dependent increase in liver GST isoenzymes gene and protein expression, compared to controls.
    In conclusion, TRF oral supplementation for 14 days resulted in increased gene and protein expression of GST isoenzymes
    in mice liver dose-dependently, with the highest expression seen in mice treated with 1000 mg/kg TRF.
    Matched MeSH terms: Chondroitin Sulfates
  3. Leanne Britcher, Sunil Kumar, Hans J. Griesser, Kim S. Siow
    Sains Malaysiana, 2018;47:1913-1922.
    In this report, we demonstrate that continuous improvement in XPS instruments and the calibration standards as well
    as analysis with standard component-fitting procedures can be used to determine the binding energies of compounds
    containing phosphorus and sulfur of different oxidation states with higher confidence. Based on such improved XPS
    analyses, the binding energies (BEs) of S2p signals for sulfur of increasing oxidation state are determined to be 166-167.5
    eV for S=O in dimethyl sulfoxide, 168.1 eV for S=O2
    in polysulfone, 168.4 eV for SO3
    in polystyrene sulfonate and 168.8
    eV for SO4
    in chondroitin sulfate. The BEs of P2p signals show the following values: 132.9 eV for PO3
    in triisopropyl
    phosphite, 133.3 eV for PO4
    in glycerol phosphate, 133.5 eV for PO4
    in sodium tripolyphosphate and 134.0 eV for PO4
    in sodium hexametaphosphate. These results showed that there are only small increases in the binding energy when
    additional oxygen atoms are added to the S-O chemical group. A similar result is obtained when the fourth oxygen or
    poly-phosphate environment is added to the phosphorus compound. These BE values are useful to researchers involved
    in identifying oxidation states of phosphorus and sulfur atoms commonly observed on modified surfaces and interfaces
    found in applications such as biomaterials, super-capacitors and catalysis.
    Matched MeSH terms: Chondroitin Sulfates
  4. Mohd Ali MKFB, Abu Bakar A, Md Noor N, Yahaya N, Ismail M, Rashid AS
    Environ Technol, 2017 Oct;38(19):2427-2439.
    PMID: 27875932 DOI: 10.1080/09593330.2016.1264486
    Microbiologically influenced corrosion (MIC) is among the common corrosion types for buried and deep-water pipelines that result in costly repair and pipeline failure. Sulfate-reducing bacteria (SRB) are commonly known as the culprit of MIC. The aim of this work is to investigate the performance of combination of ultrasound (US) irradiation and ultraviolet (UV) radiation (known as Hybrid soliwave technique, HyST) at pilot scale to inactivate SRB. The influence of different reaction times with respect to US irradiation and UV radiation and synergistic effect toward SRB consortium was tested and discussed. In this research, the effect of HyST treatment toward SRB extermination and corrosion studies of carbon steel coupon upon SRB activity before and after the treatment were performed using weight loss method. The carbon steel coupons immersed in SRB sample were exposed to HyST treatment at different time of exposure. Additionally, Field Emission Scanning Electron Microscopy coupled with Energy Dispersive X-ray Spectroscopy were used to investigate the corrosion morphology in verifying the end product of SRB activity and corrosion formation after treatment. Results have shown that the US irradiation treatment gives a synergistic effect when combined with UV radiation in mitigating the SRB consortium.
    Matched MeSH terms: Sulfates
  5. Muhammad SN, Kusin FM, Md Zahar MS, Mohamat Yusuff F, Halimoon N
    Environ Technol, 2017 Aug;38(16):2003-2012.
    PMID: 27745113 DOI: 10.1080/09593330.2016.1244568
    Passive bioremediation of metal- and sulfate-containing acid mine drainage (AMD) has been investigated in a batch study. Multiple substrates were used in the AMD remediation using spent mushroom compost (SMC), limestone, activated sludge (AS), and woodchips (WC) under anoxic conditions suitable for bacterial sulfate reduction (BSR). Limestones used were of crushed limestone (CLS) and uncrushed limestone, provided at two different ratios in mixed substrates treatment and varied by the proportion of SMC and limestone. The SMC greatly assisted the removals of sulfate and metals and also acted as an essential carbon source for BSR. The mixed substrate composed of 40% CLS, 30% SMC, 20% AS, and 10% WC was found to be effective for metal removal. Mn, Cu, Pb, and Zn were greatly removed (89-100%) in the mixed substrates treatment, while Fe was only removed at 65%. Mn was found to be removed at a greatly higher rate than Fe, suggesting important Mn adsorption onto organic materials, that is, greater sorption affinity to the SMC. Complementary with multiple treatment media was the main mechanism assisting the AMD treatment through microbial metal reduction reactions.
    Matched MeSH terms: Sulfates
  6. Watts MP, Spurr LP, Gan HM, Moreau JW
    Appl Microbiol Biotechnol, 2017 Jul;101(14):5889-5901.
    PMID: 28510801 DOI: 10.1007/s00253-017-8313-6
    Thiocyanate (SCN-) forms as a by-product of cyanidation during gold ore processing and can be degraded by a variety of microorganisms utilizing it as an energy, nitrogen, sulphur and/or carbon source. In complex consortia inhabiting bioreactor systems, a range of metabolisms are sustained by SCN- degradation; however, despite the addition or presence of labile carbon sources in most bioreactor designs to date, autotrophic bacteria have been found to dominate key metabolic functions. In this study, we cultured an autotrophic SCN--degrading consortium directly from gold mine tailings. In a batch-mode bioreactor experiment, this consortium degraded 22 mM SCN-, accumulating ammonium (NH4+) and sulphate (SO42-) as the major end products. The consortium consisted of a diverse microbial community comprised of chemolithoautotrophic members, and despite the absence of an added organic carbon substrate, a significant population of heterotrophic bacteria. The role of eukaryotes in bioreactor systems is often poorly understood; however, we found their 18S rRNA genes to be most closely related to sequences from bacterivorous Amoebozoa. Through combined chemical and phylogenetic analyses, we were able to infer roles for key microbial consortium members during SCN- biodegradation. This study provides a basis for understanding the behaviour of a SCN- degrading bioreactor under autotrophic conditions, an anticipated approach to remediating SCN- at contemporary gold mines.
    Matched MeSH terms: Sulfates/metabolism
  7. Deng YF, Liu YY, Zhang YT, Wang Y, Liang JB, Tufarelli V, et al.
    J Sci Food Agric, 2017 Jun;97(8):2382-2391.
    PMID: 27664398 DOI: 10.1002/jsfa.8050
    BACKGROUND: The efficacy and role of inulin in the mitigation of enteric sulfur-containing odor gases hydrogen sulfide (H2 S) and methyl mercaptan (CH3 SH) in pigs were examined in this study. Twelve Duroc × Landrace × Yorkshire male finisher pigs (60.7 ± 1.9 kg), housed individually in open-circuit respiration chambers, were randomly assigned to two dietary groups, namely basal diet (control) and basal diet supplemented with 1% (w/w) inulin. At the end of the 45 day experiment, pigs were slaughtered and volatile fatty acid (VFA) concentration, sulfate radical (SO42- ) concentration, population of sulfate-reducing bacteria (SRB) and expression of methionine gamma-lyase (MGL) gene were determined in contents from the caecum, colon (two segments) and rectum. Metabonomic analysis was used to compare differences in biochemical composition, and the Illumina MiSeq procedure to investigate differences in bacterial components, in the different parts of the large intestine between inulin-supplemented and inulin-free (control) groups.

    RESULTS: Inulin decreased (P < 0.05) the average daily enteric H2 S and CH3 SH production by 12.4 and 12.1% respectively. The concentrations of acetate, propionate and butyrate in the large intestinal content were significantly increased (P < 0.05) with inulin treatment, whereas valerate concentration and MGL mRNA expression decreased (P < 0.05). The growth of Lactobacillus, Butyrivibrio, Pseudobutyrivibrio, Bifidobacterium and Clostridium butyricum was stimulated, while that of Desulfovibrio, the dominant SRB, was inhibited, and there was an accumulation of SO42- in the large intestinal content of the inulin-supplemented pigs, suggesting that inulin mitigates H2 S generation from the SO42- reduction pathway by reducing the growth of SRB.

    CONCLUSION: The results showed that inulin mitigates CH3 SH generation via three methionine degradation metabolic pathways and H2 S generation from two cysteine degradation metabolic pathways, thus resulting in increased synthesis of these two sulfur-containing amino acids in the pig large intestine. © 2016 Society of Chemical Industry.

    Matched MeSH terms: Sulfates/metabolism
  8. Lee WK, Namasivayam P, Ong Abdullah J, Ho CL
    Sci Rep, 2017 04 24;7:46563.
    PMID: 28436444 DOI: 10.1038/srep46563
    Seaweeds survive in marine waters with high sulfate concentration compared to those living at freshwater habitats. The cell wall polymer of Gracilaria spp. which supplies more than 50% of the world agar is heavily sulfated. Since sulfation reduces the agar quality, it is interesting to investigate the effects of sulfate deprivation on the sulfate contents of seaweed and agar, as well as the metabolic pathways of these seaweeds. In this study, two agarophytes G. changii and G. salicornia were treated under sulfate deprivation for 5 days. The sulfate contents in the seaweed/agar were generally lower in sulfate-deprivated samples compared to those in the controls, but the differences were only statistically significant for seaweed sample of G. changii and agar sample of G. salicornia. RNA sequencing (RNA-Seq) of sulfate-deprivated and untreated seaweed samples revealed 1,292 and 3,439 differentially expressed genes (DEGs; ≥1.5-fold) in sulfate-deprivated G. changii and G. salicornia, respectively, compared to their respective controls. Among the annotated DEGs were genes involved in putative agar biosynthesis, sulfur metabolism, metabolism of sulfur-containing amino acids, carbon metabolism and oxidative stress. These findings shed light on the sulfate deprivation responses in agarophytes and help to identify candidate genes involved in agar biosynthesis.
    Matched MeSH terms: Sulfates
  9. Wu Y, Yang Z, Law JB, He AY, Abbas AA, Denslin V, et al.
    Tissue Eng Part A, 2017 01;23(1-2):43-54.
    PMID: 27824280 DOI: 10.1089/ten.TEA.2016.0123
    Stem cell differentiation is guided by contact with the physical microenvironment, influence by both topography and mechanical properties of the matrix. In this study, the combined effect of substratum nano-topography and mechanical stiffness in directing mesenchymal stem cell (MSC) chondrogenesis was investigated. Three polyesters of varying stiffness were thermally imprinted to create nano-grating or pillar patterns of the same dimension. The surface of the nano-patterned substrate was coated with chondroitin sulfate (CS) to provide an even surface chemistry, with cell-adhesive and chondro-inductive properties, across all polymeric substrates. The surface characteristic, mechanical modulus, and degradation of the CS-coated patterned polymeric substrates were analyzed. The cell morphology adopted on the nano-topographic surfaces were accounted by F-actin distribution, and correlated to the cell proliferation and chondrogenic differentiation outcomes. Results show that substratum stiffness and topographical cues affected MSC morphology and aggregation, and influenced the phenotypic development at the earlier stage of chondrogenic differentiation. Hyaline-like cartilage with middle/deep zone cartilage characteristics was generated on softer pillar surface, while on stiffer nano-pillar material MSCs showed potential to generate constituents of hyaline/fibro/hypertrophic cartilage. Fibro/superficial zone-like cartilage could be derived from nano-grating of softer stiffness, while stiffer nano-grating resulted in insignificant chondrogenesis. This study demonstrates the possibility of refining the phenotype of cartilage generated from MSCs by manipulating surface topography and material stiffness.
    Matched MeSH terms: Chondroitin Sulfates/chemistry*
  10. Akrima Abu Bakar, Muhammad Khairool Fahmy Mohd Ali, Norhazilan Md. Noor, Nordin Yahaya, Mardhiah Ismail, Ahmad Safuan A. Rashid
    Sains Malaysiana, 2017;46:1323-1331.
    Baram Delta Operation had been producing oil and gas since 1960's and serious pipelines failure was reported in the year of 2005. The final investigation has concluded that one of the species of bacteria that has been identified to cause microbiologically influenced corrosion, specifically known as sulfate reducing bacteria (SRB) was found to be one of the potential contributing factors to the incidents. This work investigates the potential use of ultraviolet (UV) radiation to inhibit the SRB consortium that was cultivated from the crude oil in one of the main trunk lines at Baram Delta Operation, Sarawak, Malaysia. The impact of UV exposure to bio-corrosion conditions on carbon steel coupon in certain samples for 28 days was discussed in this study. The samples were exposed to UV radiation based on variations of parameters, namely: time of UV exposure; and power of UV lamp. The significant changes on the amount of turbidity reading and metal loss of the steel coupon were recorded before and after experiment. The results showed that SRB growth has reduced rapidly for almost 90% after the UV exposure for both parameters as compared to the abiotic samples. Metal loss values were also decreased in certain exposure condition. Additionally, field emission scanning electron microscopy (FESEM) coupled with energy dispersive spectroscopy (EDS) was performed to observe the biofilm layer formed on the metal surface after its exposure to SRB. The evidence suggested that the efficiency of UV treatment against SRB growth could be influenced by the particular factors studied
    Matched MeSH terms: Sulfates
  11. Irwan, J.M., Teddy, T.
    MyJurnal
    Concrete durability determines service life of structures. It can though, be weakened by aggressive environmental conditions. For instance, bio-corrosion process is due to the presence and activity of microorganisms which produce sulphuric acid to form sulphate deterioration of concrete materials. The problems related to durability and repair systems are due to lack of suitable concrete materials. The use bacteria for concrete repairing and plugging of pores and cracking in concrete has been recently explored. Previous studies had proved the possibility of using specific bacteria via bio concrete as a sustainable method for improving concrete properties. Thus, lack of information on the application of bio concrete exposed to extreme condition was the motivation for this research.
    Matched MeSH terms: Sulfates
  12. Teddy, T., Irwan, J.M., Othman, N.
    MyJurnal
    Strength and durability are important characteristics of concrete and desired engineering properties. Exposure to aggressive environment threatens durability of concrete. Previous studies on bio-concrete using several types of bacteria, including sulphate reduction bacteria (SRB), had to increase durability of concrete have shown promising results. This study used mixtures designed according to concrete requirement for sea water condition with SRB composition of 3%, 5% and 7% respectively. The curing time were 28, 56 and 90 days respectively. The mechanical properties, namely compressive strength and water permeability, were tested using cube samples. The results showed compressive strength had higher increase than the control at 53.9 Mpa. The SRB with 3%composition had maximum water permeability. Thus, adding SRB in concrete specimens improves compressive strength and water permeability. This is particularly suitable for applications using chloride ion penetration (sea water condition) where corrosion tends to affect durability of concrete constructions.
    Matched MeSH terms: Sulfates
  13. Hammed, A. M., Jaswir, I., Simsek, S., Alam, Z., Amid, A.
    MyJurnal
    This study involves extraction of sulfated polysaccahride (SP) from brown seaweed (Turbinaria turbinata). Eight processing conditions affecting enzyme aided extraction (EAE) were screened using Plackett-Burman design. Three significant factors (hydrolysis time, enzyme concentration and extraction stage) were optimized using Faced Centred Central Composite Design in Random Surface Methods. Micrograph obtained using Field Emission Scanning Electron Microscopy revealed that cellulase degradation ruptured the seaweed cell matrix thus caused increase in the release of SP. The optimum conditions for extraction of SP from T. turbinata are: extraction stage of 2, hydrolysis time of 19.5 h and enzyme concentration of 1.5 μl/ml to produce 25.13% yield. The SP obtained from cellulase treated T. turbinata is a suitable anti-inflammatory agent for pharmaceutical applications.
    Matched MeSH terms: Sulfates
  14. Lin CK, Bashir MJ, Abu Amr SS, Sim LC
    Water Sci Technol, 2016 Dec;74(11):2675-2682.
    PMID: 27973372
    The aim of the current study is to evaluate the effectiveness of combined persulphate with hydrogen peroxide (S2O8(2-)/H2O2) oxidation as a post-treatment of biologically treated palm oil mill effluent (POME) for the first time in the literature. The removal efficiencies of chemical oxygen demand (COD), ammoniacal nitrogen (NH3-N), and suspended solids (SS) were 36.8%, 47.6%, and 90.6%, respectively, by S2O8(2-) oxidation alone under certain operation conditions (i.e., S2O8(2-) = 0.82 g, pH 11, and contact time 20 min). Nevertheless, the combined process (S2O8(2-)/H2O2) achieved 75.8% and 87.1% removals of NH3-N and SS, respectively, under 2.45/1.63 g/g H2O2/S2O8(2-), pH 11, and 20 min oxidation. Moreover, 56.9% of COD was removed at pH 8.4.
    Matched MeSH terms: Sulfates/chemistry*
  15. Rostam MA, Kamato D, Piva TJ, Zheng W, Little PJ, Osman N
    Cell Signal, 2016 08;28(8):956-66.
    PMID: 27153775 DOI: 10.1016/j.cellsig.2016.05.002
    Hyperelongation of glycosaminoglycan chains on proteoglycans facilitates increased lipoprotein binding in the blood vessel wall and the development of atherosclerosis. Increased mRNA expression of glycosaminoglycan chain synthesizing enzymes in vivo is associated with the development of atherosclerosis. In human vascular smooth muscle, transforming growth factor-β (TGF-β) regulates glycosaminoglycan chain hyperelongation via ERK and p38 as well as Smad2 linker region (Smad2L) phosphorylation. In this study, we identified the involvement of TGF-β receptor, intracellular serine/threonine kinases and specific residues on transcription factor Smad2L that regulate glycosaminoglycan synthesizing enzymes. Of six glycosaminoglycan synthesizing enzymes, xylosyltransferase-1, chondroitin sulfate synthase-1, and chondroitin sulfotransferase-1 were regulated by TGF-β. In addition ERK, p38, PI3K and CDK were found to differentially regulate mRNA expression of each enzyme. Four individual residues in the TGF-β receptor mediator Smad2L can be phosphorylated by these kinases and in turn regulate the synthesis and activity of glycosaminoglycan synthesizing enzymes. Smad2L Thr220 was phosphorylated by CDKs and Smad2L Ser250 by ERK. p38 selectively signalled via Smad2L Ser245. Phosphorylation of Smad2L serine residues induced glycosaminoglycan synthesizing enzymes associated with glycosaminoglycan chain elongation. Phosphorylation of Smad2L Thr220 was associated with XT-1 enzyme regulation, a critical enzyme in chain initiation. These findings provide a deeper understanding of the complex signalling pathways that contribute to glycosaminoglycan chain modification that could be targeted using pharmacological agents to inhibit the development of atherosclerosis.
    Matched MeSH terms: Chondroitin Sulfates/metabolism
  16. Hilles AH, Abu Amr SS, Hussein RA, El-Sebaie OD, Arafa AI
    J Environ Manage, 2016 Jan 15;166:493-8.
    PMID: 26580899 DOI: 10.1016/j.jenvman.2015.10.051
    A combination of persulfate and hydrogen peroxide (S2O8(2-)/H2O2) was used to oxidizelandfill leachate. The reaction was performed under varying S2O8(2-)/H2O2 ratio (g/g), S2O8(2-)/H2O2 dosages (g/g), pH, and reaction time (minutes), so as to determine the optimum operational conditions. Results indicated that under optimum operational conditions (i.e. 120 min of oxidation using a S2O8(2-)/H2O2 ratio of 1 g/1.47 g at a persulfate and hydrogen peroxide dosage of 5.88 g/50 ml and8.63 g/50 ml respectively, at pH 11) removal of 81% COD and 83% NH3-N was achieved. In addition, the biodegradability (BOD5/COD ratio) of the leachate was improved from 0.09 to 0.17. The results obtained from the combined use of (S2O8(2-)/H2O2) were compared with those obtained with sodium persulfate only, hydrogen peroxide only and sodium persulfate followed by hydrogen peroxide. The combined method (S2O8(2-)/H2O2) achieved higher removal efficiencies for COD and NH3-N compared with the other methods using a single oxidizing agent. Additionally, the study has proved that the combination of S2O8(2-)/H2O2 is more efficient than the sequential use of sodium persulfate followed by hydrogen peroxide in advanced oxidation processes aiming at treatingstabilizedlandfill leachate.
    Matched MeSH terms: Sulfates
  17. Hilles AH, Abu Amr SS, Hussein RA, Arafa AI, El-Sebaie OD
    Water Sci Technol, 2016;73(1):102-12.
    PMID: 26744940 DOI: 10.2166/wst.2015.468
    The objective of this study was to investigate the performance of employing H2O2 reagent in persulfate activation to treat stabilized landfill leachate. A central composite design (CCD) with response surface methodology (RSM) was applied to evaluate the relationships between operating variables, such as persulfate and H2O2 dosages, pH, and reaction time, to identify the optimum operating conditions. Quadratic models for the following two responses proved to be significant with very low probabilities (<0.0001): chemical oxygen demand (COD) and NH3-N removal. The obtained optimum conditions included a reaction time of 116 min, 4.97 g S2O8(2-), 7.29 g H2O2 dosage and pH 11. The experimental results were corresponding well with predicted models (COD and NH3-N removal rates of 81% and 83%, respectively). The results obtained in the stabilized leachate treatment were compared with those from other treatment processes, such as persulfate only and H2O2 only, to evaluate its effectiveness. The combined method (i.e., /S2O8(2-)/H2O2) achieved higher removal efficiencies for COD and NH3-N compared with other studied applications.
    Matched MeSH terms: Sulfates/chemistry*
  18. Willis Poratti G, Yaakop AS, Chan CS, Urbieta MS, Chan KG, Ee R, et al.
    Genome Announc, 2016;4(4).
    PMID: 27540078 DOI: 10.1128/genomeA.00870-16
    Desulfotomaculum copahuensis strain CINDEFI1 is a novel spore-forming sulfate-reducing bacterium isolated from the Copahue volcano area, Argentina. Here, we present its draft genome in which we found genes related with the anaerobic respiration of sulfur compounds similar to those present in the Copahue environment.
    Matched MeSH terms: Sulfates
  19. Jamulidin, S.N.K., Manogaran. M., Yakasai, M.H., Rahman, M.F.A., Shukor, M.Y.
    MyJurnal
    In this study, a novel glyphosate-degrading shows the ability to reduce molybdenum to
    molybdenum blue. The enzyme from this bacterium was partially purified and partially
    characterized to ascertain whether the Mo-reducing enzyme from this bacterium shows better or
    lower efficiency in reducing molybdenum compared to other Mo-reducing bacterium that only
    exhibits a single biotransformation activity. The enzyme was partially purified using ammonium
    sulphate fractionation. The Vmax for the electron donating substrate or NADH was at 1.905 nmole
    Mo blue/min while the Km was 6.146 mM. The regression coefficient was 0.98. Comparative
    assessment with the previously characterized Mo-reducing enzyme from various bacteria showed
    that the Mo-reducing enzyme from Burkholderia vietnamiensis strain AQ5-12 showed a lower
    enzyme activity.
    Matched MeSH terms: Sulfates
  20. Shamshuddin J, Panhwar Q, Shazana M, Elisa A, Fauziah C, Naher U
    Sains Malaysiana, 2016;45:383-392.
    Acid sulfate soils are generally not suitable for the crop production unless they are efficiently improved. A study was conducted to improve the productivity of acid sulfate soils for rice cultivation using ground magnesium limestone (GML), basalt and organic fertilizer. The study was conducted on rice in laboratory, glasshouse and field. The pH of acid sulfate soils was low and exchangeable Al was very high which affected rice growth. The application of GML and basalt increased soil pH and reduced Al toxicity. GML required to ameliorate the soils for rice cultivation was 4 t ha-1. Basalt in combination with organic fertilizer was a good soil amendment, but required to be applied a few months ahead of rice cultivation. Due to GML or basalt application, rice plants grew well even though water pH was below 5. The highest rice yield obtained was 4.0 t ha-1 season-1 for Sulfaquepts and it was 7.5 t ha-1 season-1 for Sulfosaprists. In general, the application of GML or basalt in combination with organic fertilizer improved the productivity of acid sulfate soils and consequently enhanced rice yield.
    Matched MeSH terms: Sulfates
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