Displaying publications 641 - 660 of 4697 in total

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  1. Fulltext Case report: two human Streptococcus suis infections in Borneo, Sabah, Malaysia
    Rajahram GS, Hameed AA, Menon J, William T, Tambyah PA, Yeo TW
    BMC Infect Dis, 2017 03 04;17(1):188.
    PMID: 28257622 DOI: 10.1186/s12879-017-2294-z
    BACKGROUND: Streptococcus Suis (S.suis) is increasingly being recognised as a potentially preventable emerging zoonotic infection in humans with a global distribution. It is a major cause of meningitis especially among those in contact with pigs and has also been associated with a toxic shock syndrome.

    CASE PRESENTATIONS: We report the first two human cases from Sabah, Borneo, Malaysia which expands the global reach of this important pathogen. Here, we illustrate their epidemiological risk factors, clinical presentation and resulting sequelae of both patients.

    CONCLUSION: The continued public health threat of zoonotic infections such as S.suis, highlights the need for accurate epidemiological surveillance, regulation of pig farming, slaughtering and continued advocacy of best practices for pork preparation and consumption.

    Matched MeSH terms: Streptococcus suis/isolation & purification*
  2. In Vitro Antifungal Susceptibility of Neoscytalidium dimidiatum Clinical Isolates from Malaysia
    James JE, Santhanam J, Lee MC, Wong CX, Sabaratnam P, Yusoff H, et al.
    Mycopathologia, 2017 Apr;182(3-4):305-313.
    PMID: 27815659 DOI: 10.1007/s11046-016-0085-5
    Neoscytalidium dimidiatum is an opportunistic fungus causing cutaneous infections mostly, which are difficult to treat due to antifungal resistance. In Malaysia, N. dimidiatum is associated with skin and nail infections, especially in the elderly. These infections may be mistaken for dermatophyte infections due to similar clinical appearance. In this study, Neoscytalidium isolates from cutaneous specimens, identified using morphological and molecular methods (28 Neoscytalidium dimidiatum and 1 Neoscytalidium sp.), were evaluated for susceptibility towards antifungal agents using the CLSI broth microdilution (M38-A2) and Etest methods. Amphotericin B, voriconazole, miconazole and clotrimazole showed high in vitro activity against all isolates with MIC ranging from 0.0313 to 1 µg/mL. Susceptibility towards fluconazole and itraconazole was noted in up to 10% of isolates, while ketoconazole was inactive against all isolates. Clinical breakpoints for antifungal drugs are not yet available for most filamentous fungi, including Neoscytalidium species. However, the results indicate that clinical isolates of N. dimidiatum in Malaysia were sensitive towards miconazole, clotrimazole, voriconazole and amphotericin B, in vitro.
    Matched MeSH terms: Ascomycota/isolation & purification
  3. [Enterovirus type 70, a pathogenic agent of acute haemorrhagic conjunctivitis (author's transl)]
    Kopecká D
    Cesk Epidemiol Mikrobiol Imunol, 1977 Nov;25(6):342-4.
    PMID: 188556
    Matched MeSH terms: Enterovirus/isolation & purification
  4. Letter: Liver fluke in a Malaysian Orang Asli (Aborigine)
    Prathap K
    Trans R Soc Trop Med Hyg, 1973;67(6):881-2.
    PMID: 4788767
    Matched MeSH terms: Trematoda/isolation & purification*
  5. Nematode parasites of Oceanica. XVI. Cordonema venusta gen. et. sp.nov., and Skrjabinoclava spp. (Acuariidae: Echinuriinae), from birds
    Schmidt GD, Kuntz RE
    Parasitology, 1972 Apr;64(2):235-44.
    PMID: 5018477
    Matched MeSH terms: Spiruroidea/isolation & purification
  6. A presumptive case of naturally occurring Plasmodium knowlesi malaria in man in Malaysia
    Fong YL, Cadigan FC, Coatney GR
    Trans R Soc Trop Med Hyg, 1971;65(6):839-40.
    PMID: 5003320
    Matched MeSH terms: Plasmodium/isolation & purification
  7. Studies of bacterial disease in West Malaysian. Orang Asli (aborigines): previously unrecorded Salmonella Serotypes
    Anandan J, Lim TW, Haug NL
    Med J Malaya, 1969 Jun;23(4):269-71.
    PMID: 4242174
    Matched MeSH terms: Salmonella/isolation & purification*
  8. Three cases of Ascaris lumbricoides small bowel obstruction with volvulus
    Roe TN, Lal K, Cawkell WA
    Med J Malaya, 1968 Sep;23(1):78-9.
    PMID: 4237564
    Matched MeSH terms: Ascaris/isolation & purification
  9. Arbovirus infections in Sarawak: further observations on mosquitoes
    Macdonald WW, Smith CE, Dawson PS, Ganapathipillai A, Mahadevan S
    J Med Entomol, 1967 May;4(2):146-57.
    PMID: 4383192
    Matched MeSH terms: Encephalitis Viruses/isolation & purification*
  10. A survey of Salmonella VI-phage types occurring in Federation of Malaya and Singapore
    Singh RB
    Med J Malaya, 1966 Mar;20(3):215-20.
    PMID: 4223129
    Matched MeSH terms: Salmonella typhi/isolation & purification*
  11. Lanjan virus, a new agent isolated from Dermacentor auratus in Malaya
    Tan DS, Smith CE, McMahon DA, Bowen ET
    Nature, 1967 Jun 10;214(5093):1154-5.
    PMID: 4964058
    Matched MeSH terms: Insect Viruses/isolation & purification*
  12. Plasmodium inui-like infection producing a presumably fatal illness in a leaf monkey Presbytis johnii--a previously undescribed host
    Das SK, Strauss JM, Chee CT
    Med J Malaya, 1966 Jun;20(4):323.
    PMID: 4224342
    Matched MeSH terms: Plasmodium/isolation & purification
  13. A double infection of filariasis in a black panther (Panthera pardus) from Pahang
    Strauss JM, Sivanandam S
    Med J Malaya, 1966 Jun;20(4):336.
    PMID: 4224351
    Matched MeSH terms: Filarioidea/isolation & purification
  14. Group B streptococcal infection in the newborn
    Kwan PW, Khoo BH, Lam KL, Puthucheary SD
    Med J Malaysia, 1979 Sep;34(1):71-5.
    PMID: 396463
    Matched MeSH terms: Streptococcus agalactiae/isolation & purification
  15. Letter: Sarcocystis in the Malaysian long-tailed monkey, Macaca irus
    Prathap K
    Trans R Soc Trop Med Hyg, 1973;67(4):615.
    PMID: 4206526
    Matched MeSH terms: Sarcocystis/isolation & purification*
  16. Recurrence of influenza with A2-Hong Kong-68 within two months
    Kamath S, Omar M, Wah LT
    Med J Malaya, 1970 Dec;25(2):123-7.
    PMID: 4251133
    Matched MeSH terms: Orthomyxoviridae/isolation & purification
  17. Nematode parasites of Oceanica. 8. Filariids of birds, with a new genus and four new species
    Schmidt GD, Kuntz RE
    Parasitology, 1970 Apr;60(2):313-26.
    PMID: 5427102
    Matched MeSH terms: Filarioidea/isolation & purification
  18. Fulltext A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains
    Benacer D, Zain SNM, Lewis JW, Khalid MKNM, Thong KL
    Rev Soc Bras Med Trop, 2017 Mar-Apr;50(2):239-242.
    PMID: 28562762 DOI: 10.1590/0037-8682-0364-2016
    INTRODUCTION:: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains.

    METHODS:: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively.

    RESULTS:: The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water.

    CONCLUSIONS:: Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.
    Matched MeSH terms: Leptospira/isolation & purification
  19. Current advances and future visions on bioelectronic immunosensing for prostate-specific antigen
    Ibau C, Md Arshad MK, Gopinath SCB
    Biosens Bioelectron, 2017 Dec 15;98:267-284.
    PMID: 28689113 DOI: 10.1016/j.bios.2017.06.049
    Early cancer diagnosis remains the holy-grail in the battle against cancers progression. Tainted with debates and medical challenges, current therapeutic approaches for prostate cancer (PCa) lack early preventive measures, rapid diagnostic capabilities, risk factors identification, and portability, i.e. the inherent attributes offered by the label-free biosensing devices. Electronic assisted immunosensing systems inherit the high sensitivity and specificity properties due to the predilection of the antigen-antibody affinity. Bioelectronic immunosensor for PCa has attracted much attentions among the researchers due to its high-performance, easy to prepare, rapid feedback, and possibility for miniaturization. This review explores the current advances on bioelectronic immunosensors for the detection of PCa biomarker revealed in the past decade. The research milestones and current trends of the immunosensors are reported to project the future visions in order to propel their "lab-to-market" realization.
    Matched MeSH terms: Prostate-Specific Antigen/isolation & purification*
  20. Effects of plasticizers on the physicochemical properties of kappa-carrageenan films extracted from Eucheuma cottonii
    Ili Balqis AM, Nor Khaizura MAR, Russly AR, Nur Hanani ZA
    Int J Biol Macromol, 2017 Oct;103:721-732.
    PMID: 28528954 DOI: 10.1016/j.ijbiomac.2017.05.105
    The physicochemical properties of κ-carrageenan films extracted from Eucheuma cottonii (E. cottonii) incorporated with different concentrations and types of plasticizers were studied. Glycerol, sorbitol, and polyethylene glycol-300 (PEG-300) in the range of 10-60% were used as plasticizers. The results showed that the thickness and moisture content (MC) of films increased significantly (p≤0.05) with the increase in plasticizer concentration. Sorbitol-plasticized films had the lowest values. Sorbitol-plasticized films have better mechanical properties and the lowest water vapor permeability (WVP), solubility and water uptake ratio (WUR) compared with glycerol and PEG-plasticized films (p≤0.05). Fourier transform infrared (FTIR) spectra showed the intermolecular reactions between κ-carrageenan and the plasticizers in the films. Scanning electron microscopy (SEM) observations indicated that sorbitol-plasticized films have a compact structure, even at the highest concentration. The melting temperature (Tm) of films decreased (p≤0.05) with an increase in the plasticizer concentration. Here, the glycerol-plasticized films had the lowest values. X-ray diffraction (XRD) showed broad and narrow peaks of the un-plasticized κ-carrageenan film at 2θ=20.0° and 2θ=8.4°, respectively. The intensity of the broad peak increased and the narrow peak disappeared as the concentration of plasticizers increased. In conclusion, films from E. cottonii successfully produced with sorbitol as the plasticizer exhibited good physical properties as packaging films.
    Matched MeSH terms: Carrageenan/isolation & purification*
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