Displaying publications 101 - 120 of 144 in total

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  1. Determination of Aflatoxins B1 and B2 in Peanuts and Corn Based Products
    Loh SH, Nur Iryani Mohd Yusof, How ML
    Sains Malaysiana, 2010;39.
    A method for the determination of aflatoxins B1 and B2 in peanuts and corn based products is described. The samples were extracted with a mixture of acetonitrile-water (84:16), followed by multifunctional clean-up and liquid chromatography with fluorescence detection. Both calibration curves showed good correlation from 4.0 to 32.0 ppb for aflatoxin B1 (r=0.9999) and 1.2 to 9.6 ppb for aflatoxin B2 (r=0.9997). The detection limit of aflatoxins B1 and B2 were established at 0.1 and 0.03 ppb, respectively, based on signal-to-noise ratio of 3:1. Average recoveries for the determination of aflatoxins B1 and B2 at 10 and 3 ppb spiking levels, respectively ranged from 94.2 to 107.6%. A total of 20 peanut samples and corn based products were obtained from retail shop and local market around Kuala Terengganu and analyzed for aflatoxins B1 and B2 contents, using the proposed method. Aflatoxins B1 and B2 were detected in 5 out of the 9 peanuts samples and 5 out of the 11 corn based products, at levels ranging from 0.2 to 101.8 ppb.
    Matched MeSH terms: Zea mays
  2. Frying quality of virgin coconut oil as affected by Zea mays extract
    Marina A, Wan Rosli WI, Neoh S
    Sains Malaysiana, 2014;43:1311-1315.
    The effect of corn silk or Zea mays extract on the physicochemical changes of virgin coconut oil was studied during three consecutive days of deep frying. There were three types of oil blend systems used: virgin coconut oil without antioxidant as control system; virgin coconut oil with butylated hydroxytoluene (BHT) as synthetic antioxidant and virgin coconut oil with Zea mays extract as natural antioxidant. The oil quality was assessed by measuring the colour, viscosity, peroxide, p-anisidine, free fatty acids, totox and iodine value. The results show that Zea mays extract delayed the oil deterioration. The Zea mays extract significantly (p<0.05) lowered the rate of oxidation in virgin coconut oil compared to control oil and was comparable to BHT. Zea mays extract did not change the sensory profiles of French fries which was shown by insignificant difference (p<0.05) between Zea mays and control fries for all sensory attributes (colour, taste, aroma, crispiness, oiliness and overall quality). In general, the Zea may extract was capable of extending the stability and quality of virgin coconut oil and therefore has potential as new source of natural antioxidant for use in deep frying.
    Matched MeSH terms: Zea mays
  3. Nutritional composition of selected commercial biscuits in Malaysia
    Norhayati M, Mohd Fairulnizal M, Aswir A, Ang J, Mohd Naeem M, Suraiami M, et al.
    Sains Malaysiana, 2015;44:581-591.
    The objective of this study was to determine the nutrient composition of selected commercial biscuits in Malaysia. A total
    of six brands from each type of biscuit (chocolate chip, corn, cracker with sugar, vegetable flavoured cracker, cream
    filled, oatmeal, shortbread and fully coated chocolate wafer) and five brands of wholemeal crackers were sampled
    from local supermarkets in the Klang Valley. The total energy content in commercial biscuits was ranging from 453.30
    to 499.25 kcal/100 g. Carbohydrate was the major macronutrient in commercial biscuits ranging from 56.86 to 66.07
    g/100 g. The highest protein content was found in the wholemeal crackers (9.92 g/100 g) and the lowest protein content
    was found in the cream filled biscuits (5.65 g/100 g). Fat content lay in the range of 16.89 to 25.75 g/100 g. The major
    minerals detected in biscuits were sodium, followed by calcium and magnesium. The vitamin content in A and E of the
    biscuits examined was almost comparable. Small quantities of trans fatty acids were detected in all biscuits in the range
    of 0.02 to 0.68 g/100 g. These findings indicate that Malaysian commercial biscuits are a good source of carbohydrate
    (34.12-39.64 g per serving), calcium (26.09-384.67 g per serving), magnesium (10.42-37.24 g per serving) and contain
    low proportion of trans fatty acids (0.01-0.41 g per serving).
    Matched MeSH terms: Zea mays
  4. Differential response of Zea mays L. in relation to weed control and different macronutrient combinations
    Khan M, Kakar S, Marwat K, Khan I
    Sains Malaysiana, 2013;42:1395-1401.
    Time of weed control and fertilizer application usually decide the profitability of crop production. The effects of weed control and macronutrients on maize crop were investigated. The study was undertaken in March 2009, using a RCBD design with split plot arrangements. The experimental set up was established at the Agricultural University Peshawar and seedbeds were prepared with the proper moisture regime. Maize was planted with one plot left weed free for first six weeks while another infested with weed. The combinations of macronutrients used were nitrogen, phosphorus, potassium, nitrogen-phosphorus, nitrogen-potassium, phosphorus-potassium and nitrogen-phosphorus-potassium. Control (no fertilizer) was included for comparison. The observations revealed that when a comparison was made between the application of fertilizers and weed control, the latter proved more important because weed infested plots had no harvestable maize plants. The role of main nutrients in crop production is well known and cannot be left aside, however weed infestation does not provide us a fair choice of fertilizers application. The maximum maize grain yield was recorded under nitrogen-phosphorus combination and promising results were obtained. The weeds and maize benefited equally in terms of fresh and dry weed biomass with an application of fertilizer in particular N singly or together with P. In view of this, application of fertilizer should be changed from broadcast to band and/or placement. In general, a positive interaction was seen between N and P promoting the growth of maize and weeds. It can be said that herbicide application for weed control is important because of the fact that hand weeding is not economical, difficult, time consuming because of perennial weeds and hot weather conditions in the month of June.
    Matched MeSH terms: Zea mays
  5. Yield potential of spring wheat influenced by crop residue, tillage system and nitrogen rate on irrigated land
    Asad Shah, Mohammad Akmal, Mohammad Jamal Khan, Mohammad Arif
    Sains Malaysiana, 2014;43:1811-1819.
    Yield performance in wheat (Triticum aestivum) was compared under crop residue, tillage system and nitrogen rate treatments in cereal based cropping system. The experiments were conducted at Agronomy Research Farm, The University of Agriculture, Peshawar, in 2009-2010 and 2010-2011. Chopped crop residue on dry matter basis (5 t ha1-) of legume (Vigna unguicuata, var. Ebney) and cereal (Zea mays, var. Azam) was applied in main plots with no residue treatments and plowed with Mould Board (MB) and Cultivator as deep and shallow treatments, respectively. A month after the crop residue and tillage system treatments, field was uniformly plowed with cultivator and wheat was sown with drill in rows 25 cm apart in the month of November on both years. Both P2O55 and K2O (80 and 40 kg ha , respectively) were applied uniformly to all fields before sowing. Nitrogen as subplot treatment (0, 40, 80, 120 and 160 kg ha-1) was applied in two splits, half at 15 and the other half at 45 days after sowing with uniform cultural practices for crop growth and development. Compared to year 1, crop of year 2 showed better phenology with extended life cycle (LC). On two years average across tillage and N treatments, biological yield did not change (p<0.05) under the residue but did report lower at no-residue treatment. Nonetheless, grain yield showed a significant (p<0.05) change with the highest in legume followed by cereal and the lowest in no-residue treatments. A non-significant tiller number and significant variations in grain weight and spike m-2 were observed that influenced the grain and biological yield differently. Deep than shallow tillage resulted in better traits, which returned better biomass and grain yield. Nitrogen application from control to every increment showed a significant (p<0.05) improvement in all observations contributing in yield. The study confirms the significance of legume vs. cereal over no-crop residue incorporated through deep tillage system with optimum N (120 kg ha-1) in cereal-based cropping system for sustainable performance to sustain soil C:N for future production.
    Matched MeSH terms: Zea mays
  6. Fulltext Nitrogen Pollution Impact and Remediation through Low Cost Starch Based Biodegradable polymers
    Ibrahim KA, Naz MY, Shukrullah S, Sulaiman SA, Ghaffar A, AbdEl-Salam NM
    Sci Rep, 2020 Apr 03;10(1):5927.
    PMID: 32246028 DOI: 10.1038/s41598-020-62793-3
    The world does not have too much time to ensure that the fast-growing population has enough land, food, water and energy. The rising food demand has brought a positive surge in fertilizers' demand and agriculture-based economy. The world is using 170 million tons of fertilizer every year for food, fuel, fiber, and feed. The nitrogenous fertilizers are being used to meet 48% of the total food demand of the world. High fertilizer inputs augment the reactive nitrogen levels in soil, air, and water. The unassimilated reactive nitrogen changes into a pollutant and harms the natural resources. The use of controlled-release fertilizers for slowing down the nutrients' leaching has recently been practiced by farmers. However, to date, monitoring of the complete discharge time and discharge rate of controlled released fertilizers is not completely understood by the researchers. In this work, corn starch was thermally processed into a week gel-like coating material by reacting with urea and borate. The granular urea was coated with native and processed starch in a fluidized bed reactor having bottom-up fluid delivery system. The processed starch exhibited better thermal and mechanical stability as compared to the native starch. Unlike the pure starch, the storage modulus of the processed starch dominated the loss modulus. The release time of urea, coated with processed starch, remained remarkably larger than the uncoated urea.
    Matched MeSH terms: Zea mays
  7. Profiling of anti-oxidative enzymes and lipid peroxidation in leaves of salt tolerant and salt sensitive maize hybrids under nac1 and cd stress
    Ghulam Hasan Abbasi, Javaid Akhtar, Muhammad Anwar-ul-haq, Moazzam Jamil, Shafaqat Ali, Rafiq Ahmad, et al.
    Sains Malaysiana, 2016;45:177-184.
    Effects of NaCl salinity and cadmium on the anti-oxidative activity of enzymes like superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), glutathione reductase (GR) and lipid peroxidation contents; malondialdehyde (MDA) were studied in two maize hybrids of different salt tolerance characteristics. An increase in the amount of lipid peroxidation indicated the oxidative stress induced by NaCl and Cd. The results also depicted that NaCl stress caused an increase in the activities of POD, SOD, CAT, APX and GR while cadmium stress increased the activities of POD, SOD and APX but showed no significant effect on CAT and GR in both the studied hybrids. The combined effect of salinity and cadmium on these parameters was higher than that of sole effect of either NaCl or Cd. It was also found that maize hybrid 26204 had better tolerance against both stresses with strong antioxidant system as compared to that of maize hybrid 8441. A comparison of the antioxidants and lipid peroxidation in two maize hybrids having varying level of NaCl and Cd stress tolerance corroborated the importance of reactive oxygen species (ROS) in defense against abiotic stresses.
    Matched MeSH terms: Zea mays
  8. Characterization of fusarium proliferatum and fusarium verticillioides based on species-specific gene and microsatellites analysis
    Najihah A, Nur Ain Izzati M, Yong S, Nik Mohd Izham M
    Sains Malaysiana, 2017;46:2425-2432.
    Fusarium species are known to cause various diseases on plantations including fruits and vegetables. The most common Fusarium that can cause plant diseases are Fusarium proliferatum and Fusarium verticillioides. Ear rot disease on maize, wilt disease on cucurbits and fruit rot disease on tomato as well as banana are example of diseases caused by these two species. The objectives of this study were to identify F. proliferatum and F. verticillioides based on species-specific primers and polymerase chain reaction (PCR) amplification and to evaluate the genetic diversity of both species based on microsatellite markers. Fifty isolates of Fusarium species that were previously collected throughout Malaysia from different hosts were identified by using species-specific PCR amplification. Twenty-nine isolates were identified as F. proliferatum and 21 isolates were identified as F. verticillioides based on species-specific primer. The genetic diversity of all the fungal isolates was evaluated by using microsatellite analysis with six established primers. Five out of six primers amplified polymorphic bands with the most effective primer showing high polymorphism were (AG)7C and (TCC)5 meanwhile one primer (TTTC)4 gave negative result with no band amplified. The phylogenetic tree that was constructed showing two different clades distinguished between F. proliferatum and F. verticillioides.
    Matched MeSH terms: Zea mays
  9. Chabrolene, a novel norditerpene from the bornean soft coral nephthea sp.
    Takahiro Ishii, Takashi Kamada, Chin-soon Phan, Charles S. Vairappan
    Sains Malaysiana, 2018;47:319-322.
    Soft corals are often the research interest organism due to their structural diverse secondary metabolites with wide
    spectrum of biological activities range from antibacterial to anticancer potentials. The Borneo Island is rich in diversity
    of marine organisms including soft coral. Therefore, a population of Bornean soft coral belongs to genus Nephthea
    was collected from Mantanani Island (Sabah, Malaysia) which led to the isolation of a novel norditerpene, chabrolene
    (1) together with three known compounds (2-4). The chemical structure of 1 was determined by NMR and HREIMS data.
    Compound 1 exhibited repellent activity against the maize weevil Sitophilus zeamais.
    Matched MeSH terms: Zea mays
  10. Identification of drought tolerant maize genotypes and seedling based morpho-physiological selection indices for crop improvement
    Fahad Masoud Wattoo, Rashid Mehmood Rana, Sajid Fiaz, Syed Adeel Zafar, Mehmood Ali Noor, Shoaib ur Rehman, et al.
    Sains Malaysiana, 2018;47:295-302.
    Maize is an imperative grain crop used as a staple food in several countries around the world. Water deficiency is a serious
    problem limiting its growing area and production. Identification of drought tolerant maize germplasm is comparatively
    easy and sustainable approach to combat this issue. Present research was conducted to evaluate 50 maize genotypes
    for drought tolerance at early growth stage. Drought tolerance was assessed on the basis of several morphological
    and physiological parameters. Analysis of variance showed significant variation among the tested maize genotypes for
    recorded parameters. Principal component analysis revealed important morpho-physiological traits that were playing
    key role in drought tolerance. Correlation studies depicted significant positive correlation among the attributes such as
    fresh shoot length (FSL), fresh root length (FRL), dry shoot weight (DSW), dry root weight (DRW), relative water contents
    (RWC) and total dry matter (TDM) while a strongly negative correlation was observed among RWC and excised leaf
    water loss. Results concluded that the parameters fresh shoot weight, fresh root weight, FRL, DRW, TDM, cell membrane
    thermo stability (CMT) and RWC can be useful for rapid screening of maize germplasm for drought tolerance at early
    growth stages. Furthermore, the genotypes 6, 16, 18, 40, 45 and 50 can be used as a drought tolerant check in breeding
    programs. Moreover, biplot analysis along with other indices was proved to be a useful approach for rapid and cost
    efficient screening of large number of genotypes against drought stress condition.
    Matched MeSH terms: Zea mays
  11. Jatropha curcas leaves mulch effect on seedling emergence and growth of maize (zea mays)
    Irshad M, Ullah F, Mehmood S, Khan AU
    Sains Malaysiana, 2016;45:1013-1018.
    Allelopathy is a process in which one plant species may usefully or adversely affect the growth of other plant species
    through the production of allelochemicals. During the present investigation, mulch effect of Jatropha curcas leaves was
    evaluated on seed germination and seedling growth of maize varieties viz. Pioneer (V1), Azam (V2) and Jalal (V3). Mulch
    was applied at 1 and 2 tons/hectare. Phenolic compounds were detected in Jatropha curcas leaf (131.15 mg gallic acid
    eq./gm extract). Mulch applied at 2 tons/hectare significantly reduced seed germination (%), germination index, relative
    water content, root width and seedling dry weight. From the findings of the present investigation, it was inferred that
    Jatropha curcas leaves exhibited phytotoxic effects on maize at high concentrations.
    Matched MeSH terms: Zea mays
  12. Fulltext Electrochemical Immunosensor for the Detection of Aflatoxin B₁ in Palm Kernel Cake and Feed Samples
    Azri FA, Selamat J, Sukor R
    Sensors (Basel), 2017 Nov 30;17(12).
    PMID: 29189760 DOI: 10.3390/s17122776
    Palm kernel cake (PKC) is the solid residue following oil extraction of palm kernels and useful to fatten animals either as a single feed with only minerals and vitamins supplementation, or mixed with other feedstuffs such as corn kernels or soy beans. The occurrence of mycotoxins (aflatoxins, ochratoxins, zearalenone, and fumonisins) in feed samples affects the animal's health and also serves as a secondary contamination to humans via consumption of eggs, milk and meats. Of these, aflatoxin B₁ (AFB₁) is the most toxically potent and a confirmed carcinogen to both humans and animals. Methods such as High Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS/MS) are common in the determination of mycotoxins. However, these methods usually require sample pre-treatment, extensive cleanup and skilled operator. Therefore, in the present work, a rapid method of electrochemical immunosensor for the detection of AFB₁ was developed based on an indirect competitive enzyme-linked immunosorbent assay (ELISA). Multi-walled carbon nanotubes (MWCNT) and chitosan (CS) were used as the electrode modifier for signal enhancement.N-ethyl-N'-(3-dimethylaminopropyl)-carbodiimide (EDC) andN-hydroxysuccinimide (NHS) activated the carboxyl groups at the surface of nanocomposite for the attachment of AFB₁-BSA antigen by covalent bonding. An indirect competitive reaction occurred between AFB₁-BSA and free AFB₁ for the binding site of a fixed amount of anti-AFB₁ antibody. A catalytic signal based on horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H₂O₂) and 3,3',5,5'-tetramethylbenzidine (TMB) mediator was observed as a result of attachment of the secondary antibody to the immunoassay system. As a result, the reduction peak of TMB(Ox)was measured by using differential pulse voltammetry (DPV) analysis. Based on the results, the electrochemical surface area was increased from 0.396 cm² to 1.298 cm² due to the electrode modification with MWCNT/CS. At the optimal conditions, the working range of the electrochemical immunosensor was from 0.0001 to 10 ng/mL with limit of detection of 0.1 pg/mL. Good recoveries were obtained for the detection of spiked feed samples (PKC, corn kernels, soy beans). The developed method could be used for the screening of AFB₁ in real samples.
    Matched MeSH terms: Zea mays
  13. Fulltext Agricultural residues for cellulolytic enzyme production by Aspergillus niger: effects of pretreatment
    Salihu A, Abbas O, Sallau AB, Alam MZ
    3 Biotech, 2015 Dec;5(6):1101-1106.
    PMID: 28324400 DOI: 10.1007/s13205-015-0294-5
    Different agricultural residues were considered in this study for their ability to support cellulolytic enzyme production by Aspergillus niger. A total of eleven agricultural residues including finger millet hulls, sorghum hulls, soybean hulls, groundnut husk, banana peels, corn stalk, cassava peels, sugarcane bagasse, saw dust, rice straw and sheanut cake were subjected to three pretreatment (acid, alkali and oxidative) methods. All the residues supported the growth and production of cellulases by A. niger after 96 h of incubation. Maximum cellulase production was found in alkali-treated soybean hulls with CMCase, FPase and β-glucosidase yields of 9.91 ± 0.04, 6.20 ± 0.13 and 5.69 ± 0.29 U/g, respectively. Further studies in assessing the potential of soybean hulls are being considered to optimize the medium composition and process parameters for enhanced cellulase production.
    Matched MeSH terms: Zea mays
  14. Effect of soluble corn fibre and calcium supplementation on bone mineral content and bone mineral density in preadolescent Malaysian children-a double-blind randomised controlled trial (PREBONE-Kids Study)
    Arasu K, Chang CY, Wong SY, Ong SH, Yang WY, Chong MHZ, et al.
    Osteoporos Int, 2023 Apr;34(4):783-792.
    PMID: 36808216 DOI: 10.1007/s00198-023-06702-0
    Soluble corn fibre (SCF) with calcium did not improve bone indices after 1 year in preadolescent children.

    INTRODUCTION: SCF has been reported to improve calcium absorption. We investigated the long-term effect of SCF and calcium on bone indices of healthy preadolescent children aged 9-11 years old.

    METHODS: In a double-blind, randomised, parallel arm study, 243 participants were randomised into four groups: placebo, 12-g SCF, 600-mg calcium lactate gluconate (Ca) and 12-g SCF + 600-mg calcium lactate gluconate (SCF + Ca). Total body bone mineral content (TBBMC) and total body bone mineral density (TBBMD) were measured using dual-energy X-ray absorptiometry at baseline, 6 and 12 months.

    RESULTS: At 6 months, SCF + Ca had a significant increase in TBBMC from baseline (27.14 ± 6.10 g, p = 0.001). At 12 months, there was a significant increase in TBBMC from baseline in the SCF + Ca (40.28 ± 9.03 g, p = 0.001) and SCF groups (27.34 ± 7.93 g, p = 0.037). At 6 months, the change in TBBMD in the SCF + Ca (0.019 ± 0.003 g/cm2) and Ca (0.014 ± 0.003 g/cm2) groups was significantly different (p 

    Matched MeSH terms: Zea mays
  15. Selective adsorption of organic dyes from aqueous environment using fermented maize extract-enhanced graphene oxide-durian shell derived activated carbon composite
    Obayomi KS, Yon Lau S, Danquah MK, Zhang J, Chiong T, Meunier L, et al.
    Chemosphere, 2023 Oct;339:139742.
    PMID: 37562502 DOI: 10.1016/j.chemosphere.2023.139742
    A secure aquatic environment is essential for both aquatic and terrestrial life. However, rising populations and the industrial revolution have had a significant impact on the quality of the water environment. Despite the implementation of strong and adapted environmental policies for water treatment worldwide, the issue of organic dyes in wastewater remains challenging. Thus, this study aimed to develop an efficient, cost-effective, and sustainable material to treat methylene blue (MB) in an aqueous environment. In this research, maize extract solution (MES) was utilized as a green cross-linker to induce precipitation, conjugation, and enhance the adsorption performance of graphene oxide (GO) cross-linked with durian shell activated carbon (DSAC), resulting in the formation of a GO@DSAC composite. The composite was investigated for its adsorptive performance toward MB in aqueous media. The physicochemical characterization demonstrated that the cross-linking method significantly influenced the porous structure and surface chemistry of GO@DSAC. BET analysis revealed that the GO@DSAC exhibited dominant mesopores with a surface area of 803.67 m2/g. EDX and XPS measurements confirmed the successful cross-linking of GO with DSAC. The adsorption experiments were well described by the Harkin-Jura model and they followed pseudo-second order kinetics. The maximum adsorption capacity reached 666.67 mg/g at 318 K. Thermodynamic evaluation indicated a spontaneous, feasible, and endothermic in nature. Regenerability and reusability investigations demonstrated that the GO@DSAC composite could be reused for up to 10 desorption-adsorption cycles with a removal efficiency of 81.78%. The selective adsorptive performance of GO@DSAC was examined in a binary system containing Rhodamine B (RhB) and methylene orange (MO). The results showed a separation efficiency (α) of 98.89% for MB/MO and 93.66% for MB/RhB mixtures, underscoring outstanding separation capabilities of the GO@DSAC composite. Overall, the GO@DSAC composite displayed promising potential for the effective removal of cationic dyes from wastewater.
    Matched MeSH terms: Zea mays
  16. Utilization of bivalve shell-treated Zea mays L. (maize) husk leaf as a low-cost biosorbent for enhanced adsorption of malachite green
    Jalil AA, Triwahyono S, Yaakob MR, Azmi ZZ, Sapawe N, Kamarudin NH, et al.
    Bioresour Technol, 2012 Sep;120:218-24.
    PMID: 22820110 DOI: 10.1016/j.biortech.2012.06.066
    In this work, two low-cost wastes, bivalve shell (BS) and Zea mays L. husk leaf (ZHL), were investigated to adsorb malachite green (MG) from aqueous solutions. The ZHL was treated with calcined BS to give the BS-ZHL, and its ability to adsorb MG was compared with untreated ZHL, calcined BS and Ca(OH)(2)-treated ZHL under several different conditions: pH (2-8), adsorbent dosage (0.25-2.5 g L(-1)), contact time (10-30 min), initial MG concentration (10-200 mg L(-1)) and temperature (303-323 K). The equilibrium studies indicated that the experimental data were in agreement with the Langmuir isotherm model. The use of 2.5 g L(-1) BS-ZHL resulted in the nearly complete removal of 200 mg L(-1) of MG with a maximum adsorption capacity of 81.5 mg g(-1) after 30 min of contact time at pH 6 and 323 K. The results indicated that the BS-ZHL can be used to effectively remove MG from aqueous media.
    Matched MeSH terms: Zea mays/chemistry*
  17. Transformation of oil palm using Agrobacterium tumefaciens
    Izawati AM, Parveez GK, Masani MY
    Methods Mol Biol, 2012;847:177-88.
    PMID: 22351008 DOI: 10.1007/978-1-61779-558-9_15
    Transgenic oil palm (Elaeis guineensis Jacq.) plantlets are regenerated after Agrobacterium tumefaciens-mediated transformation of embryogenic calli derived from young leaves of oil palm. The calli are transformed with an Agrobacterium strain, LBA4404, harboring the plasmid pUBA, which carries a selectable marker gene (bar) for resistance to the herbicide Basta and is driven by a maize ubiquitin promoter. Modifications of the transformation method, treatment of the target tissues using acetosyringone, exposure to a plasmolysis medium, and physical injury via biolistics are applied. The main reasons for such modifications are to activate the bacterial virulence system and, subsequently, to increase the transformation efficiency. Transgenic oil palm cells are selected and regenerated on a medium containing herbicide Basta. Molecular analyses revealed the presence and integration of the introduced bar gene into the genome of the transformants.
    Matched MeSH terms: Zea mays/genetics
  18. Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system
    Soleimany F, Jinap S, Rahmani A, Khatib A
    Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 2011 Apr;28(4):494-501.
    PMID: 21337232 DOI: 10.1080/19440049.2010.551547
    A new method for the simultaneous quantification of 12 mycotoxins was developed and optimized using reverse phase high performance liquid chromatography (RP-HPLC) with a photodiode array (PDA) and fluorescence detector (FLD), a photochemical reactor for enhanced detection (PHRED) and post-column derivatization. The mycotoxins included aflatoxins (AFB(1), AFB(2), AFG(1), and AFG(2)), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), fumonisins (FB(1), FB(2), and FB(3)), T-2 and HT-2 toxins. A double sample extraction with a phosphate-buffered saline solution (PBS) and methanol was used for co-extraction of mycotoxins, and a multifunctional immunoaffinity column was used for cleanup. Optimum conditions for separation of the mycotoxins were obtained to separate 12 mycotoxins in FLD and PDA chromatograms with a high resolution. The method gave recoveries in the range 72-111% when applied to spiked corn samples. The limits of detection (LOD) were 0.025 ng/g for AFB(1) and AFG(1), 0.012 ng/g for AFB(2) and AFG(2), 0.2 ng/g for OTA, 1.5 ng/g for ZEA, 6.2 ng/g for FB(1), FB(3) and HT-2 toxin, 9.4 ng/g for FB(2) and T-2 toxin, and 18.7 ng/g for DON. In addition, the limits of quantification (LOQ) ranged from 0.04 ng/g for AFB(2) and AFG(2) to 62 ng/g for DON. The method was successfully applied to the determination of these mycotoxins in 45 cereal samples obtained from the Malaysian market. The results indicated that the method can be applied for the multi-mycotoxin determination of cereals.
    Matched MeSH terms: Zea mays/chemistry
  19. Dual modification of starch via partial enzymatic hydrolysis in the granular state and subsequent hydroxypropylation
    Karim AA, Sufha EH, Zaidul IS
    J Agric Food Chem, 2008 Nov 26;56(22):10901-7.
    PMID: 18975963 DOI: 10.1021/jf8015442
    The effect of enzymatic pretreatment on the degree of corn and mung bean starch derivatization by propylene oxide was investigated. The starch was enzymatically treated in the granular state with a mixture of fungal alpha-amylase and glucoamylase at 35 degrees C for 16 h and then chemically modified to produce enzyme-hydrolyzed-hydroxypropyl (HP) starch. Partial enzyme hydrolysis of starch in the granular state appeared to enhance the subsequent hydroxypropylation, as judged from the significant increase in the molar substitution. A variable degree of granule modification was obtained after enzyme hydrolysis, and one of the determinants of the modification degree appeared to be the presence of natural pores in the granules. Enzyme-hydrolyzed-HP starch exhibited significantly different functional properties compared to hydroxypropyl starch prepared from untreated (native) starch. It is evident that the dual modification of starch using this approach provides a range of functional properties that can be customized for specific applications.
    Matched MeSH terms: Zea mays/chemistry
  20. Fulltext Influence of Pyrolysis Temperature on Physico-Chemical Properties of Corn Stover (Zea mays L.) Biochar and Feasibility for Carbon Capture and Energy Balance
    Rafiq MK, Bachmann RT, Rafiq MT, Shang Z, Joseph S, Long R
    PLoS One, 2016;11(6):e0156894.
    PMID: 27327870 DOI: 10.1371/journal.pone.0156894
    This study examined the influence of pyrolysis temperature on biochar characteristics and evaluated its suitability for carbon capture and energy production. Biochar was produced from corn stover using slow pyrolysis at 300, 400 and 500°C and 2 hrs holding time. The experimental biochars were characterized by elemental analysis, BET, FTIR, TGA/DTA, NMR (C-13). Higher heating value (HHV) of feedstock and biochars was measured using bomb calorimeter. Results show that carbon content of corn stover biochar increased from 45.5% to 64.5%, with increasing pyrolysis temperatures. A decrease in H:C and O:C ratios as well as volatile matter, coupled with increase in the concentration of aromatic carbon in the biochar as determined by FTIR and NMR (C-13) demonstrates a higher biochar carbon stability at 500°C. It was estimated that corn stover pyrolysed at 500°C could provide of 10.12 MJ/kg thermal energy. Pyrolysis is therefore a potential technology with its carbon-negative, energy positive and soil amendment benefits thus creating win- win scenario.
    Matched MeSH terms: Zea mays/chemistry*
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