Kajian ini dijalankan untuk membentuk formula demi menganggar ketinggian tubuh badan dan menentukan jantina dengan menggunakan panjang dan lebar kaki. Seramai 150 orang subjek yang berumur 20-30 tahun diambil dari Universiti Kebangsaan Malaysia Kampus Kuala Lumpur (UKMKKL). Subjek tersebut adalah Melayu (45 lelaki dan 45 perempuan) dan Cina (30 lelaki dan 30 perempuan). Ketinggian, panjang dan lebar kaki diukur menggunakan alatan antropometrik dalam ukuran milimeter. Hasil statistik menunjukkan bahawa pelbagai ukuran kaki kanan dan kiri tidak mempunyai perbezaan yang signifikan (Panjang kaki/FL: t(298) = -0.235, p = 0.815; Lebar kaki/FW: t(298) = 0.932, p = 0.352). Perbezaan yang signifikan pada semua ukuran pula dapat dilihat pada jantina (Tinggi/S: t(148) = 12.550, p < 0.05; FL:t(148) = 11.692, p < 0.05; FW:t(148) = 8.321, p < 0.05). Bagi kaum, ketinggian tubuh badan kaum Cina adalah lebih tinggi berbanding kaum Melayu secara signifikan (t(148) = -3.103, p < 0.05) tetapi tiada perbezaan yang signifikan diperhatikan pada ukuran kaki (FL: t(148) = 0.002, p = 0.999; FW:t(148) = 1.362, p = 0.175). Korelasi yang paling tinggi telah ditunjukkan antara ketinggian tubuh badan dengan panjang kaki pada semua kumpulan. Analisis Regresi Garis Lurus dan Regresi Berganda telah digunakan untuk membentuk formula menganggar ketinggian tubuh badan. Walau bagaimanapun, didapati formula yang dibentuk dari Regresi Berganda adalah lebih tepat kerana kombinasi panjang dan lebar kaki menghasilkan korelasi koefisen yang lebih tinggi dalam semua formula (S = 585.298+(4.898xFL)-(1.453xFW), F(2,149 = 149.324, p < 0.01, R2 = 0.670). Formula untuk penentuan jantina juga telah dibina menggunakan Analisis Pokok Klasifikasi. Keputusan menunjukkan ketepatan untuk menentukan jantina seseorang adalah di antara 86.3-87.5% bagi perempuan dan 85.2-85.7% bagi lelaki dengan menggunakan panjang kaki manakala di antara 68.4-76.8% bagi perempuan dan 77.8-81.0% bagi lelaki dengan menggunakan lebar kaki.
Antibiotics which once a boon in medicine and saved millions of lives are now facing an ever-growing menace of antibacterial resistance, which desperately needs new antibacterial drugs which are innovative in chemistry and mode of action. For many years, the world has turned to natural plants with antibacterial properties to combat antibiotic resistance. On that basis, we aimed to identify plants with antibacterial and antibiotic potentiating properties. Seventeen different extracts of 3 plants namely Burkillanthus malaccensis, Diospyros hasseltii and Cleisthanthus bracteosus were tested against multi-drug resistant Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Methicillinresistant Staphylococcus aureus (MRSA) and methicillin-susceptible Staphylococcus aureus (MSSA). Antibacterial activity of hexane, methanol and chloroform extracts of bark, seed, fruit, flesh and leaves from these plants were tested using, disk diffusion assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. Antibiotic potentiating capabilities were tested using time-kill assay. B. malaccensis fruit chloroform extract showed the biggest zone of inhibition against MRSA (13.00±0.0 mm) but C. bracteosus bark methanol extract showed the biggest inhibition zone against MSSA (15.33±0.6 mm). Interestingly, bark methanol extract of C. bracteosus was active against MRSA (8.7±0.6 mm), MSSA (7.7±0.6 mm) (Gram-positive) and A. baumannii (7.7±0.6 mm) (Gram-negative). Overall, the leaf methanol and bark methanol extract of C. bracteosus warrants further investigation such as compound isolation and mechanism of action for validating its therapeutic use as antibiotic potentiator importantly against MRSA and A. baumannii.
Fruit used in the common human diet in general, and kiwifruit and persimmon particularly, displays health properties in the prevention of heart disease. This study describes a combination of bioactivity, multivariate data analyses and fluorescence measurements for the differentiating of kiwifruit and persimmon, their quenching and antioxidant properties. The metabolic differences are shown, as well in the results of bioactivities and antioxidant capacities determined by ABTS, FRAP, CUPRAC and DPPH assays. To complement the bioactivity of these fruits, the quenching properties between extracted polyphenols and human serum proteins were determined by 3D-fluorescence spectroscopy studies. These properties of the extracted polyphenols in interaction with the main serum proteins in the human metabolism (human serum albumin (HSA), α-β-globulin (α-β G) and fibrinogen (Fgn)), showed that kiwifruit was more reactive than persimmon. There was a direct correlation between the quenching properties of the polyphenols of the investigated fruits with serum human proteins, their relative quantification and bioactivity. The results of metabolites and fluorescence quenching show that these fruits possess multiple properties that have a great potential to be used in industry with emphasis on the formulation of functional foods and in the pharmaceutical industry. Based on the quenching properties of human serum proteins with polyphenols and recent reports in vivo on human studies, we hypothesize that HSA, α-β G and Fgn will be predictors of coronary artery disease (CAD).
Persimmon (Diospyros kaki L.) is one of the most important fruits that has been consumed for its medicinal properties due to the presence of some active metabolites, particularly polyphenols and carotenoids. Previously described methods, including HPLC, were limited in the determination of metabolites in different persimmon varieties. The present study shows the evaluation and the differences among persimmon polar and non-polar extracts by 1H NMR-based metabolomics approach. The hierarchical clustering analysis (HCA) based on score values of principal component analysis (PCA) model was used to analyze the important compounds in investigated fruits. The 1H NMR spectrum of persimmon chloroform (CDCl3) extracts showed different types of compounds as compared to polar methanol-water (CD3OD-D2O) ones. Persimmons growing in Israel were clustered different from those growing in Korea with the abundance of phenolic compounds (gallic, caffeic and protocathecuic acids), carotenoids (β-cryptoxanthin, lutein, and zeaxanthin), amino acids (alanine), maltose, uridine, and fatty acids (myristic and palmitoleic acids). Glucose, choline and formic acid were more prominent in persimmon growing in Korea. In CD3OD-D2O and CDCl3 persimmon extracts, 43 metabolites were identified. The metabolic differences were shown as well on the results of bioactivities and antioxidant capacities determined by ABTS, FRAP, CUPRAC and DPPH assays. The presented methods can be widely used for quantitation of multiple compounds in many plant and biological samples especially in vegetables and fruits.
A 1.7 m2 flat plate solar air heater was designed and developed in the Department of Agricultural Mechanization, The University of Agriculture Peshawar, Pakistan in collaboration with the Department of Environmental Sciences, University of Peshawar, Pakistan. It was operated under an average solar irradiance of 0.9 kJ.m-2.h-1 in the month of September, 2013. It worked under an efficiency of 7.5 to 21%. The heat collected by the air heater was given to air flowing as a medium inside it. This hot air was given to a drying section and water heating tank for drying and water heating purposes. The drying section provided a temperature in the range of 40-50oC and humidity of 10-30% from 10:00 am to 3:00 pm. The water heating tank provides hot water with a temperature of 35- 45oC from 10:00 am to 5:00 pm. Furthermore, the drying section was used to dry apples, onions and persimmons. All the products showed a consistent moisture loss from them with an optimum drying rate. The two term exponential model showed that all the three products dried have a good correlation with drying time with R2 values higher than 0.90. It was concluded that flat plate solar air heaters is the best technology for water heating and drying purposes and is a good alternative of conventional energy sources.