Displaying publications 1 - 20 of 36 in total

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  1. [A study of the antigenicity of group A streptococcus strain "Matsuyama 2166" and its prevalence]
    Murai T, Inazumi Y
    Kansenshogaku Zasshi, 1989 Nov;63(11):1223-30.
    PMID: 2480985
    Antigen analysis of group A streptococcus strain, "Matsuyama 2166", which had been typed as T12-28 (by the T-agglutination method), MNT (nontypeable by the M-precipitation method) using conventional typing sera was examined by the precipitation and precipitation absorption technique. The prevalence of group A streptococci, typed under the name "Matsuyama 2166" was also investigated. The results were as follows: 1) The strain "Matsuyama 2166" is OF (Opacity Factor) (+) and its serological components consist of M "Matsuyama 2166" antigen, which is unique in our collection of M-types, and T28 antigen as a major antigen along with T12 as a minor. 2) Out of the group A streptococci typed as MNT, 96.6% of the streptococci were types as T12-28 and 96.5% of T28 by conventional typing sera were strains to be typed as M "Matsuyama 2166" using the newly prepared M "Matsuyama 2166" typing serum, suggesting the great advantage to M-typing rate of T28 strains. 3) Group A streptococcus M "Matsuyama 2166" was also found in isolates from Thailand or Malaysia. It is interesting that T-types found in those isolates seems to be a little different from the T-types isolated in Japan. These results showed that there was the difficult problem when we did the speculation of M-type from the result of T-type.
    Matched MeSH terms: Streptococcus pyogenes/immunology*; Streptococcus pyogenes/isolation & purification
  2. Diagnosis of Group A streptococcal pharyngitis by enzyme immunoassay
    Zooraidah Z, Farida J
    Family Physician, 1990;2:27-29.
    Matched MeSH terms: Streptococcus pyogenes
  3. Group A streptococcal infections
    Jamal F
    Malays J Pathol, 1996 Jun;18(1):15-6.
    PMID: 10879218
    Matched MeSH terms: Streptococcus pyogenes*
  4. Fulltext New emm (M protein gene) sequences of group A streptococci isolated from Malaysian patients
    Jamal F, Pit S, Facklam R, Beall B
    Emerg Infect Dis, 1999 Jan-Feb;5(1):182-3.
    PMID: 10081694
    Matched MeSH terms: Streptococcus pyogenes/classification; Streptococcus pyogenes/genetics*; Streptococcus pyogenes/isolation & purification
  5. A study of haemolytic streptococci isolated from outpatients in dermatological clinics
    Murai T, Inazumi Y, Nishiwaki M, Noda Y, Hino H
    Kansenshogaku Zasshi, 1991 Aug;65(8):960-9.
    PMID: 1919131
    A total of 44 patients suspected of streptococcal infections were studied in outpatient clinics in Tokyo during the one year from December 1988 to December 1989. Employing bacteriological culturing and serodiagnosis, the following results were obtained. 1) There were 9 cases of impetigo and 15 cases of erysipelas with typical clinical manifestations and age distributions. 2) It seemed that some of the skin infections were caused by group A streptococci whose M-types were different from those of upper respiratory infections typically occurring in Japan. 3) The type distribution of group A streptococci found were quite similar to those isolated in Thailand or Malaysia. 4) There were found group A streptococci exhibiting unique combinations of T- and M-types, such as T11 and M9, T11 and M62 or T13-49 and MOD8 (Provisional type). 5) As for serodiagnostic method, ADNB (anti-deoxyribonuclease B) titer reflected infection by group A streptococcus only, while ASK (anti-streptokinase) and ASO (anti-streptolysin O) reflected not only group A streptococcal infections but group G infections as well.
    Matched MeSH terms: Streptococcus pyogenes/classification*; Streptococcus pyogenes/immunology
  6. Acanthamoeba castellanii interactions with Streptococcus pneumoniae and Streptococcus pyogenes
    Siddiqui R, Yee Ong TY, Jung SY, Khan NA
    Exp Parasitol, 2017 Dec;183:128-132.
    PMID: 28823705 DOI: 10.1016/j.exppara.2017.08.005
    Among the genus Streptococcus, S. pyogenes and S. pneumoniae are the major causes of pharyngitis, impetigo, pneumonia and meningitis in humans. Streptococcus spp. are facultative anaerobes that are nutritionally fastidious, yet survive in the environment and target the predisposed population. Antibacterial disinfectants have been partially effective only, indicating the need for novel preventative measures and to understand mechanisms of bacterial resistance. Acanthamoeba is a free-living protist that is known to harbour microbial pathogens, provide shelter, and assist in their transmission to susceptible population. The overall aim of this study was to determine whether S. pyogenes and S. pneumoniae can interact with A. castellanii by associating, invading, and surviving inside trophozoites and cysts. It was observed that both S. pyogenes and S. pneumoniae were able to associate as well as invade and/or taken up by the phagocytic A. castellanii trophozoite. Notably, S. pyogenes and S. pneumoniae survived the encystation process, avoided phagocytosis, multiplied, and exhibited higher recovery from the mature cysts, compared with the trophozoite stage (approximately 2 bacteria per amoebae ratio for cyst stage versus 0.02 bacteria per amoeba ration for trophozoite stage). As Acanthamoeba cysts are resilient and can disperse through the air, A. castellanii can act as a vector in providing shelter, facilitating growth and possibly genetic exchanges. In addition, these interactions may contribute to S. pyogenes and S. pneumoniae survival in harsh environments, and transmission to susceptible population and possibly affecting their virulence. Future studies will determine the molecular mechanisms associated with Acanthamoeba interactions with Streptococcus and the evolution of pathogenic bacteria and in turn expedite the discovery of novel therapeutic and/or preventative measures.
    Matched MeSH terms: Streptococcus pyogenes/growth & development; Streptococcus pyogenes/physiology*
  7. Characterization of group A streptococci isolated in Kuala Lumpur, Malaysia
    Jamal F, Pit S, Johnson DR, Kaplan EL
    J Trop Med Hyg, 1995 Oct;98(5):343-6.
    PMID: 7563264
    T-agglutination patterns of 190 strains of group A streptococci isolated between January 1989 and December 1993 from body fluids (10), throat culture (56), pus (51) and skin lesions (73) were determined. Mucoid colonial morphology was exhibited by 6.3% (12/190) of the strains on initial isolation. Type T-5,11,27,44 comprised 23.7%, followed by T-1,3,13,B3264 (11.1%), T-4,6 (8.4%) and T-8,25, Imp 19 (7.9%). About 42% (80/190) strains could not be characterized by T agglutination pattern. T-typing of 71 selected strains at WHO Collaborating Center, Minneapolis yielded similar results. Nineteen selected strains were further characterized by M-typing; only three strains were M-typeable. These strains were isolated from throat (M1), sputum (M5) and pus (M12). About 68% (48/71) isolates produced serum opacity factor. These data support the existence of as yet uncharacterized group A streptococcal serotypes in this region.
    Matched MeSH terms: Streptococcus pyogenes/classification*; Streptococcus pyogenes/cytology; Streptococcus pyogenes/isolation & purification
  8. Studies on variation in Lancefield group A type 50 streptococcal cell-wall carbohydrate
    Kurl DN
    Adv Exp Med Biol, 1997;418:607-10.
    PMID: 9331725
    Matched MeSH terms: Streptococcus pyogenes/classification; Streptococcus pyogenes/genetics; Streptococcus pyogenes/immunology*
  9. Prevention of Group A streptococcal infection in Malaysia
    Jamal F
    Family Practitioner, 1988;11(1):73-74.
    Group A streptococcus is an important cause of pharyngitis, skin infection and or its non-suppurative sequelae, i.e. rheumatic fever and post-streptococcal glomerulonephritis. The National Streptococcus Reference Laboratory was established in 1982 to collect data on various aspects of this infection.
    Matched MeSH terms: Streptococcus pyogenes
  10. Fulltext Pharyngeal carriage of group A Streptococcus in Malaysian schoolchildren
    Jamal F, George J, Aziz AA, Ahmad D
    Family Practitioner, 1986;9(1):38-39.
    Pharyngeal carriage of group A streptococcus was determined in 432 primary school children between the ages of 6 and 8 years. Beta-haemolytic streptococci were isolated from throat swab culture of 71 pupils, with a carrier rate of 16.4% (71/432) of which 9.4% (39/432) belonged to Lancefield's group A. Serogrouping of the isolates was determined by the coagglutination method and Lancefield's hot acid extraction method. 54.9% (39/71) of the total beta-hemolytic streptococci isolated belonged to group A , 25.3% (18/71) to group G, 15.4% (11/71) to group C and 1.4%(1/71) to group F. T typing pattern of group A streptococcus was determined by the standard agglutination method. Sensitivity to antibiotics was determined by the disc diffusion technique (comparative method). All group A streptococcal isolates were sensitive to penicillin and erythromycin, 6 strains (15.4%) were resistant to tetracycline and 1 strain (2.5%) was resistant to cephaloridine.
    Matched MeSH terms: Streptococcus pyogenes
  11. Significance of enteric gram-negative bacilli in the throat
    Philpot CR, McDonald PJ, Chai KH
    J Hyg (Lond), 1980 Oct;85(2):205-10.
    PMID: 7005325
    Pharyngeal micro-organisms of 131 Australian and Malaysian children and adults were compared by analysis of aerobic culture of throat swab specimens. Enteric Gram-negative bacilli were commonly isolated in small numbers from Malaysian adults whether they had sore throats (28%) or not (36%), but were detected in only 9% of Australian adults without sore throats and in only 12% and 4% of Malaysian children with and without sore throats respectively. In other respects microbiological findings were similar in the different groups of subjects studied. It is concluded that the pharyngeal carriage rate of enteric Gram-negative bacilli may differ substantially between different groups of normal individuals. Our findings also suggest that these micro-organisms do not have a pathogenic role in pharyngitis.
    Matched MeSH terms: Streptococcus pyogenes/isolation & purification
  12. Pharyngeal exudates: mere mantle or menace?
    Nathan PS, Jagathesan M
    Med J Malaysia, 1976 Jun;30(4):264-5.
    PMID: 790110
    Matched MeSH terms: Streptococcus pyogenes/isolation & purification
  13. Streptococcal impetigo among aboriginal children in Malaysia
    Norazah A, Normaznah Y, Kamel AG, Rohani MY
    Southeast Asian J Trop Med Public Health, 1995 Dec;26(4):803-4.
    PMID: 9139400
    Matched MeSH terms: Streptococcus pyogenes*
  14. Fulltext New insights into the mode of action of the lantibiotic salivaricin B
    Barbour A, Tagg J, Abou-Zied OK, Philip K
    Sci Rep, 2016 08 16;6:31749.
    PMID: 27526944 DOI: 10.1038/srep31749
    Salivaricin B is a 25 amino acid polycyclic peptide belonging to the type AII lantibiotics and first shown to be produced by Streptococcus salivarius. In this study we describe the bactericidal mode of action of salivaricin B against susceptible Gram-positive bacteria. The killing action of salivaricin B required micro-molar concentrations of lantibiotic whereas the prototype lantibiotic nisin A was shown to be potent at nano-molar levels. Unlike nisin A, salivaricin B did not induce pore formation or dissipate the membrane potential in susceptible cells. This was established by measuring the fluorescence of the tryptophan residue at position 17 when salivaricin B interacted with bacterial membrane vesicles. The absence of a fluorescence blue shift indicates a failure of salivaricin B to penetrate the membranes. On the other hand, salivaricin B interfered with cell wall biosynthesis, as shown by the accumulation of the final soluble cell wall precursor UDP-MurNAc-pentapeptide which is the backbone of the bacterial peptidoglycan. Transmission electron microscopy of salivaricin B-treated cells showed a reduction in cell wall thickness together with signs of aberrant septum formation in the absence of visible changes to cytoplasmic membrane integrity.
    Matched MeSH terms: Streptococcus pyogenes/drug effects; Streptococcus pyogenes/ultrastructure
  15. Fulltext Travel-related Streptococcal toxic shock syndrome caused by emm type 78 Streptococcus pyogenes
    Tappe D, Schulze MH, van der Linden M, Ziegler U, Müller A, Stich A
    J Clin Microbiol, 2011 Aug;49(8):3094-5.
    PMID: 21632896 DOI: 10.1128/JCM.02623-10
    Streptococcal toxic shock syndrome is a serious health problem in developed and developing countries. We here report a case of severe protracted disease after a minor skin infection in a young traveler returning from West Malaysia which was caused by an unusual emm-type strain harboring speG and smeZ superantigen genes.
    Matched MeSH terms: Streptococcus pyogenes/genetics; Streptococcus pyogenes/isolation & purification*
  16. Fulltext Dual bioactivities of laurus nobilis essential oil
    Nur Afiqah Saparin, Mohd Muzamir Mahat, Muhd Fauzi Safian, Shahrul Hisham Zainal Ariffin, Nor Azah Mohamad Ali, Zaidah Zainal Ariffin
    Science Letters, 2020;14(1):62-67.
    MyJurnal
    The evolution of cosmetic products results in the growing demands for cosmetics that are preservatives free. Plant essential oils were found to be a promising antimicrobial and also antioxidant agent. In this study, Cymbopogon citratus (lemongrass), Laurus nobilis (bay leaf) and Backhousia citriodora (lemon myrtle) essential oils were selected and evaluated for their antimicrobial properties. It was found that Laurus nobilis exhibited strong antimicrobial activity against the selected bacteria Streptococcus saprophyticus (ATCC 49619), Streptococcus aureus (ATCC 22923), Streptococcus pyogenes (ATCC 29436), Pseudomonas aeruginosa (ATCC 13048), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 22922) with MIC ranging between 7.8 ug/mL to 250 μg/mL. The antioxidant activity of selected essential oils was determined by antioxidant assays which were 1,1-Diphenyl-2-picrylhydrazyl assay (DPPH), determination of ferric reducing antioxidant power assay (FRAP) and β-Carotene/linoleic acid bleaching assay. Backhousia citriodora and Laurus nobilis showed the highest antioxidant activity.
    n-Octanal and β-Selinene were identified to be the major components with peak area of 26.37 % and 13.92 % respectively in secondary metabolites analysis by Gas Chromatography-Mass Spectrometry (GCMS).
    Matched MeSH terms: Streptococcus pyogenes
  17. Fulltext Group A streptococcal skin infection in childhood
    Jamal F, Minhaj AA, Kasim MS, Zainal Z, George R
    Family Practitioner, 1985;8(2):53-56.
    Bacterial skin infection was studied in 239 children between the ages of 1 and 12 years in an urban squatter area in Kuala Lumpur over a period of three months (January - March 1983). 62 children presented with dermatological problems, 33 of which were clinically diagnosed as pyoderma. Microbiological examination of swabs from the lesions yielded mixed growth from 54.5% (18/33) specimens. Beta-haemolytic streptococci were isolated from 63.6% (21/33) and Staphylococcus aureus from 54.5% (18/33) specimens. All streptococcal isolates were grouped with a commercial kit, the Streptex (Wellcome). 81% (17/21) belonged to Lancefield's group A, 90% of which were not M or T typable by the standard international set of antisera. Urine analysis, pharyngeal swab culture and determination of anti-streptococcal antibody titre were also performed for children with positive skin cultures for group A streptococcus. 6% (2/33) of children with impetigo developed acute glomerulonephritis, requiring hospitalization.
    Study site: Growth and nutrition clinic, squatter area, Kuala Lumpur, Malaysia
    Matched MeSH terms: Streptococcus pyogenes
  18. Fulltext Soy fermentation by indigenous oral probiotic Streptococcus spp. and its antimicrobial activity against oral pathogens
    How, Y. H., Ewe, J. A., Song, K. P., Kuan, C. H., Kuan, C. S., Yeo, S. K.
    International Food Research Journal, 2020;27(2):357-365.
    MyJurnal
    The present work aimed to determine the antagonistic effect of probiotic-fermented soy against oral pathogens. Indigenous oral probiotics (Streptococcus salivarius Taylor’s Univer- sity Collection Centre (TUCC) 1251, S. salivarius TUCC 1253, S. salivarius TUCC 1254, S. salivarius TUCC 1255, and S. orisratti TUCC 1253) were incorporated into soy fermentation at 37°C for 24 h. Growth characteristics, β-glucosidase activity, and total isoflavones content of Streptococcus strains following soy fermentation were analysed. Antimicrobial test of Streptococcus-fermented soy was carried out against oral pathogens Enterococcus faecalis American Type Culture Collection (ATCC) 700802, Streptococcus pyogenes ATCC 19615, and Staphylococcus aureus ATCC 25923. Streptococcus strains showed a significant increase in growth following soy fermentation. S. salivarius TUCC 1253-fermented soy showed signif- icantly higher extracellular β-glucosidase activity and amount of aglycones. S. salivarius TUCC 1253-fermented soy showed antimicrobial effect against all oral tested pathogens in both aerobic and anaerobic conditions. These results showed that S. salivarius TUCC 1253-fermented soy could potentially be used as a preventive action or alternative treatment for oral infections.

    Matched MeSH terms: Streptococcus pyogenes
  19. Fulltext Pseudomonas Aeruginosa and Streptococcus Pyogenes Exposed to Malaysian Trigona Honey In Vitro Demonstrated Downregulation of Virulence Factor
    A Al-Kafaween M, Mohd Hilmi AB, A Nagi Al-Jamal H, A Elsahoryi N, Jaffar N, Khairi Zahri M
    Iran J Biotechnol, 2020 Oct;18(4):e2542.
    PMID: 34056021 DOI: 10.30498/IJB.2020.2542
    Background: Honey has been known as a traditional medicine for centuries with its antibacterial properties. It is considered one of the most enduring substances used in wound management.

    Objectives: This study aimed to: (i) evaluate the effects of Malaysian Trigona honey on bacterial structure and (ii) assess the anti-virulence potential of this honey by examining their impacts on the expression of selected genes (involved in stress survival and biofilm formation) in a test organism.

    Materials and Methods: Trigona honey's impacts on the bacterial structure (cell morphology) and the expression profiles of select Pseudomonas Aeruginosa and Streptococcus Pyogenes genes were examined using scanning electron microscopy (SEM) and real-time PCR (RT-qPCR) analysis, respectively.

    Results: SEM showed that the decreased cell density deformed, disrupted, and damaged cells for both bacteria. RT-qPCR showed that the expression of fleN, fleQ, and fleR genes of P.aeruginosa were decreased, 4.26-fold, 3.80-fold and 2.66- fold respectively. In addition, scpA, ftsY, and emm13 of S.pyogenes were decreased, 2.87-fold, 3.24-fold, and 4.65-fold respectively.

    Conclusion: Our results indicate that Trigona honey may be an effective inhibitor and virulence modulator of P. aeruginosa and S. pyogenes via multiple molecular targets. This deduction needs to be investigated in vivo.

    Matched MeSH terms: Streptococcus pyogenes
  20. Fulltext Antimicrobial Effect of Malaysian Honey on Some Human Pathogens: an in vitro Study
    Hassanain, A.T., Alyaa, A.K., Karim, A.J.
    International Medical Journal Malaysia, 2010;9(2):15-18.
    MyJurnal
    Introduction: Honey has potent bactericidal activity against many pathogenic organisms, including various Gram-negative and Gram-positive bacteria. This study aimed to determine the antimicrobial effect of Malaysian honey against different species of human pathogens using optimized honey broth media. Materials and Methods: The antimicrobial activity of honey against standard strains of Gram-negative and Grampositive bacteria and yeast was tested in vitro by the broth dilution method using 10%-100% w/v concentrations prepared in tryptic soy broth. Results: Streptococcus pyogenes was the most sensitive pathogen, it was completely inhibited by 30% honey broth medium. The most resistant were E. faecalis, S. aureus and MRSA, but they were all completely inhibited by 80% honey broth medium. Conclusion: Honey is shown to possess antimicrobial activity against human Gram-positive and negative medically important bacteria.
    Matched MeSH terms: Streptococcus pyogenes
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