Browse publications by year: 2004

  1. Ong HT, Cheah JS
    N Z Med J, 2004 Feb 20;117(1189):U773.
    PMID: 15014562
    Numerous trials and meta-analyses have been conducted over the last five years to identify an ideal anti-hypertensive drug. These reports, and the JNC 7 and European hypertension guidelines, have generated some controversy and confusion. A review of the comparative anti-hypertensive drug trials shows that the differences between drugs are minor and not consistently demonstrated by different studies. However, much data have now accumulated on the safety and value of diuretics, beta blockers, calcium-channel blockers (CCBs), angiotensin-converting enzyme inhibitors (ACEIs), and angiotensin receptor blockers (ARBs) in reducing blood pressure and preventing clinical disease. The importance of tight blood pressure control in reducing adverse events has been clearly shown, and clinicians should concentrate on achieving target blood pressure levels, which often requires a combination of anti-hypertensive drugs. The choice of anti-hypertensive drug should be guided by the presence of concomitant clinical disease, as evidence has accumulated on the special efficacy of certain drugs in reducing damage to particular organ systems. In the absence of any associated clinical disease, it is good to initiate anti-hypertensive therapy with diuretics, provided the metabolic parameters are regularly reviewed.
    MeSH terms: Antihypertensive Agents/therapeutic use*; Clinical Trials as Topic; Humans; Hypertension/drug therapy*; Practice Guidelines as Topic
  2. Yeang HY
    Curr Opin Allergy Clin Immunol, 2004 Apr;4(2):99-104.
    PMID: 15021061
    PURPOSE OF REVIEW:
    New allergenic latex proteins have been identified, whereas further information on known latex allergens has emerged in recent years. Although prevalence figures for sensitization to the various latex allergens have been published in several studies in the past, the data have not been collated to facilitate cross-comparison.

    RECENT FINDINGS:
    Salient characteristics of the three most recently identified latex allergens, Hev b 11, 12 and 13 are described, whereas new findings on some of the previously recognized allergens are examined. Hev b 2 is viewed from the standpoint of allergenicity and protein glycosylation, Hev b 4 in relation to its biochemical identity and molecular cloning, Hev b 5 with respect to its recombinant form, and Hev b 6 in connection with conformational IgE epitopes. Reports on sensitization or allergic reaction to purified latex allergens from recent and past work are summarized. The use of latex allergens in latex allergy diagnostics is reviewed and discussed.

    SUMMARY:
    Thirteen latex allergens have been recognized by the International Union of Immunological Societies. Based on the results of published studies, native Hev b 2, recombinant Hev b 5, native or recombinant Hev b 6, native Hev b 13, and possibly native Hev b 4 are the major allergens relevant to latex-sensitized adults. Although there is an increasing tendency to identify and characterize latex allergens largely on the basis of their recombinant forms, not all such recombinant proteins have been fully validated against their native counterparts with respect to clinical significance.
    MeSH terms: Allergens/adverse effects*; Allergens/classification; Humans; Hypersensitivity, Immediate/diagnosis; Hypersensitivity, Immediate/etiology*; Immunoassay; Latex/adverse effects*; Latex/classification; Rubber/adverse effects*; Rubber/classification; Latex Hypersensitivity/diagnosis; Latex Hypersensitivity/etiology*
  3. Lo SK, Baharin BS, Tan CP, Lai OM
    J Chromatogr Sci, 2004 Mar;42(3):145-54.
    PMID: 15023251
    Separation of 1,2(2,3)- and 1,3-positional isomers of diacylglycerols (DAG) from vegetable oils by reversed-phase high-performance liquid chromatography (RP-HPLC) is investigated. The method is based on isocratic elution using 100% acetonitrile and UV detection at 205 nm. The following elution order of DAG molecular species is identified: 1,3-dilinolein < 1,2-dilinolein < 1,3-dimyristin < 1-oleoyl-3-linoleoyl-glycerol < 1,2-dimyristoyl-rac-glycerol < 1(2)-oleoyl-2(3)-linoleoyl-glycerol < 1-linolenoyl-3-stearoyl-glycerol < 1(2)-linolenoyl-2(3)-stearoyl-glycerol < 1,3-diolein < 1-palmitoyl-3-oleoyl-glycerol < 1,2-dioleoyl-sn-glycerol < 1(2)-palmitoyl-2(3)-oleoyl-glycerol < 1-linoleoyl-3-stearoyl-glycerol < 1,3-dipalmitin < 1(2)-linoleoyl-2(3)-stearoyl-glycerol < 1-oleoyl-3-stearoyl-glycerol < 1,2-dipalmitoyl-rac-glycerol < 1-palmitoyl-3-stearoyl-sn-glycerol < 1,3-distearin < 1,2-distearoyl-rac-glycerol. Linearity is observed over three orders of magnitude. Limits of detection and quantitation range 0.2-0.7 microg/mL for 1,3-dilinolein to 0.6-1.9 microg/mL for 1,2-dioleoyl-sn-glycerol, respectively. Precision and accuracy of the method are also demonstrated. The method is developed to separate mixtures of DAG molecular species produced from edible oils.
    MeSH terms: Chromatography, Gas; Chromatography, High Pressure Liquid/methods*; Diglycerides/analysis*; Isomerism; Plant Oils/chemistry*; Sensitivity and Specificity; Reproducibility of Results
  4. Arif SA, Hamilton RG, Yusof F, Chew NP, Loke YH, Nimkar S, et al.
    J Biol Chem, 2004 Jun 04;279(23):23933-41.
    PMID: 15024009
    Recurring reports of a highly allergenic 42-46-kDa protein in Hevea brasiliensis latex appeared to have been resolved with the discovery of a 43-kDa allergenic latex protein that was a homologue to patatin. However, the low to moderate prevalence of sensitization to the protein, designated Hev b 7, among latex-allergic patients could not adequately explain the frequent observations of the 42-46-kDa allergen. This led to the hypothesis that another, more allergenic protein of a similar molecular mass existed in Hevea latex. We report the isolation and purification of a 42.98-kDa latex glycoprotein showing homology to the early nodule-specific protein (ENSP) of the legumes Medicago sativa, Medicago truncatula, and Glycine max. The protein is allergenic, being recognized by immunoglobulin E (IgE) in sera from latex-allergic patients. The IgE epitope resides on the carbohydrate moiety of the protein, and the presence of a similar carbohydrate component on potato tuber patatin enables the latter to inhibit IgE binding to the ENSP homologue. The cDNA encoding the ENSP homologue was isolated by reverse transcription-PCR and cloned. The protein predicted from the cDNA sequence has 391 amino acids, the first 26 of which constitute a putative signal peptide. The deduced molecular mass of the mature protein is 40.40 kDa, while its isoelectric point is estimated at 5.0. The discrepancy between the predicted and observed molecular mass might be due to glycosylation, for which three N-sites on the protein are predicted. The purified protein showed lipase and esterase activities and may be involved in plant defense.
    MeSH terms: Medicago sativa/metabolism; Allergens/isolation & purification*; Allergens/chemistry*; Amino Acid Sequence; Epitopes/chemistry; Base Sequence; Carbohydrates/chemistry; Cloning, Molecular; DNA/chemistry; Enzyme-Linked Immunosorbent Assay; Escherichia coli/metabolism; Esterases/metabolism; Glycosylation; Humans; Immunoglobulin E/chemistry; Isoelectric Focusing; Latex/metabolism; Latex/chemistry*; Lipase/metabolism; Molecular Sequence Data; Peptides/chemistry; Plant Proteins; Protein Binding; Proteins/chemistry; RNA, Messenger/metabolism; Soybeans/metabolism; Trypsin/pharmacology; Blotting, Western; Sequence Homology, Amino Acid; Protein Structure, Tertiary; DNA, Complementary/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Spectrometry, Mass, Electrospray Ionization; Protein Sorting Signals; Medicago/metabolism; Antigens, Plant
  5. Yaacob NS, Kaderi MA, Norazmi MN
    J Clin Immunol, 2004 Mar;24(2):177-84.
    PMID: 15024185 DOI: 10.1023/B:JOCI.0000019783.61674.1d
    Type 1 diabetes is an autoimmune disease that results from the destruction of the insulin-producing pancreatic beta islet cells, probably via the influence of cytokines. However, direct correlation between the expression of selected cytokines by various immune cells at different time points during the progression of the disease has not yet been clearly demonstrated. In this study, we showed that the mRNA expression of the pro-inflammatory cytokines, TNF-alpha, IL-1 beta, IL-6, and GM-CSF, were increased while the anti-inflammatory cytokine, TGF-beta, decreased in the peritoneal macrophages of nonobese diabetic (NOD) mice. IL-6 expression however decreased when the mice became diabetic. Surprisingly the expression of IFN-gamma and IL-2 by splenic CD4+ cells were lower in 5-week-old NOD mice as compared to the nonobese diabetic resistant (NOR) control mice, but their expression was higher in older NOD mice. The expression of IL-4 and IL-10 decreased in splenic CD4-positive lymphocytes. Splenic CD8-positive lymphocytes expressed increased levels of IFN-gamma and IL-10 but the latter decreased sharply when diabetes occurred. The relevance of these findings to the pathogenesis of type 1 diabetes is discussed.
    MeSH terms: Age Factors; Animals; Diabetes Mellitus, Type 1/etiology; Diabetes Mellitus, Type 1/genetics; Diabetes Mellitus, Type 1/immunology*; Female; RNA, Messenger/analysis; RNA, Messenger/immunology; Spleen/cytology; Spleen/immunology; CD4-Positive T-Lymphocytes/immunology*; Polymerase Chain Reaction; Cytokines/biosynthesis; Cytokines/genetics*; Mice, Inbred NOD; Macrophages, Peritoneal/immunology*; CD8-Positive T-Lymphocytes/immunology*; Mice
  6. Imran Y, Zulmi W, Halim AS
    Singapore Med J, 2004 Mar;45(3):110-2.
    PMID: 15029411
    Immediate recognition of anastomotic failure is important to ensure the viability of the vascularised fibular graft. The problems associated with post-operative bone scanning and angiography for immediate detection of anastomotic failure have been described.
    MeSH terms: Anastomosis, Surgical; Angiography; Fibula/blood supply; Fibula/radionuclide imaging; Fibula/transplantation*; Graft Rejection*; Humans; Retrospective Studies; Surgical Flaps*; Skin Transplantation*; Treatment Outcome
  7. Kaur G, Naik VR, Rahman MNG
    Singapore Med J, 2004 Mar;45(3):125-6.
    PMID: 15029415
    Diffusely-infiltrating mucinous adenocarcinoma of the renal pelvis associated with lithiasis and chronic gout is reported in a 61-year-old Malay man. The patient underwent left nephrectomy and vesiculo-lithotomy. This tumour is postulated to arise in response to chronic irritation of the urothelium.
    MeSH terms: Adenocarcinoma, Mucinous/etiology*; Adenocarcinoma, Mucinous/pathology*; Adenocarcinoma, Mucinous/surgery; Gout/complications*; Hospitals, University; Humans; Kidney Calculi/etiology*; Kidney Neoplasms/etiology*; Kidney Neoplasms/pathology*; Kidney Neoplasms/surgery; Kidney Pelvis/pathology; Malaysia; Male; Middle Aged; Nephrectomy; Ureteral Calculi/etiology*
  8. Rahman MT, Jaafar H, Naik VR, Ghazali MZ, Hassan S
    Singapore Med J, 2004 Mar;45(3):130-1.
    PMID: 15029417
    The unusual presentation of a mature mediastinal cystic teratoma as an anterior neck swelling in a 29-year-old Malay woman is reported.
    MeSH terms: Adult; Diagnosis, Differential; Female; Head and Neck Neoplasms/pathology*; Head and Neck Neoplasms/surgery; Humans; Teratoma/pathology*; Teratoma/surgery
  9. Yaacob NS, Bakar RA, Norazmi MN
    Ann Clin Lab Sci, 2004;34(1):47-56.
    PMID: 15038667
    The polymerase chain reaction (PCR) is useful for amplifying specific mRNAs, particularly those present in low copy numbers. However, due to the exponential nature of the amplification process, PCR cannot readily be used to quantify gene expression. A competitive PCR technique was developed to address this shortcoming. An internal standard that is 100% homologous to, but shorter than, the target gene was constructed. The practicality of the method was demonstrated by determining the expression levels of a human transcription factor, peroxisome proliferator-activated receptor gamma 1 (hPPARgamma1) which is normally present in low copy numbers in selected cells. A mock system was used to test the accuracy and sensitivity of the method, which was subsequently used to determine the expression of this receptor in lipopolysaccharide (LPS)-activated monocytes, which are known to express hPPARgamma1 differentially during cellular activation. Densitometric analysis showed that the competitive PCR method reliably estimated the expression levels of hPPARgamma1 at the attomole (10(-18)) level in monocytes.
    MeSH terms: Genetic Vectors; Humans; Monocytes/chemistry; Plasmids; Reference Standards; Transcription Factors/analysis; Transcription Factors/genetics*; Transcription Factors/metabolism; Up-Regulation; Gene Expression; Polymerase Chain Reaction/methods*; Polymerase Chain Reaction/standards; Sequence Homology; Receptors, Cytoplasmic and Nuclear/analysis; Receptors, Cytoplasmic and Nuclear/genetics*; Receptors, Cytoplasmic and Nuclear/metabolism
  10. Chandrawathani P, Jamnah O, Adnan M, Waller PJ, Larsen M, Gillespie AT
    Vet Parasitol, 2004 Mar 25;120(3):177-87.
    PMID: 15041093
    Long-term field studies were conducted on two government managed small ruminant research farms, located in different geo-climatic regions and approximately 300 km separate from each other, on Peninsula Malaysia. The Infoternak trial (48 weeks) and the Chalok trial (43 weeks) each compared nematode parasite control in separately managed groups of young sheep, either short-term rotationally grazed around a suite of 10 paddocks in addition to receiving a daily supplement of Duddingtonia flagrans spores (Fungus Group); or similar groups of sheep being rotationally grazed alone (Control Group). The prevailing weather conditions at Infoternak farm were of below average rainfall conditions for the most of the trial. As a consequence, only very low worm infections (almost exclusively Haemonchus contortus) were acquired by the 17 sets of tracer lambs that grazed sequentially with the experimental lambs. However on all except 2 occasions in the early part of the trial, the mean tracer worm burdens were significantly lower (P < 0.05) and the experimental lambs grew significantly better (P = 0.054) in the Fungus Group. Rainfall at Chalok farm during the course of the trial was also below average. As a consequence infectivity of pastures was assumed to be relatively low based on faecal egg counts (epg) of the experimental sheep, which following an anthelmintic treatment prior to allocation, remained very low in both treatment groups. Faecal egg counts of undosed replacement lambs in the latter half of the Chalok study, showed a progressive increase in the Control Group to levels exceeding 3000 epg, whereas the Fungus Group remained static at approximately 500 epg. These results show that the deployment of the nematophagous fungus, D. flagrans, can improve the level of parasite control of sheep in the tropics above that which can be achieved by the short-term rotational grazing strategy alone.
    MeSH terms: Animals; Animal Husbandry; Body Weight; Mitosporic Fungi/growth & development*; Feces/parasitology; Female; Haemonchiasis/parasitology; Haemonchiasis/prevention & control*; Haemonchiasis/veterinary*; Haemonchus/growth & development*; Haemonchus/microbiology; Hematocrit/veterinary; Malaysia; Male; Parasite Egg Count/veterinary; Pest Control, Biological/methods*; Random Allocation; Sheep; Sheep Diseases/parasitology*; Sheep Diseases/prevention & control
  11. Pritchard LI, Sendow I, Lunt R, Hassan SH, Kattenbelt J, Gould AR, et al.
    Virus Res, 2004 May;101(2):193-201.
    PMID: 15041187
    Bluetongue viruses (BTV) were isolated from sentinel cattle in Malaysia and at two sites in Indonesia. We identified eight serotypes some of which appeared to have a wide distribution throughout this region, while others were only isolated in Malaysia or Australia. Nearly half of the 24 known BTV serotypes have now been identified in Asia. Further, we investigated the genetic diversity of their RNA segments 3 and 10. Using partial nucleotide sequences of the RNA segment 3 (540 bp) which codes for the conserved core protein (VP3), the BTV isolates were found to be unique to the previously defined Australasian topotype and could be further subdivided into four distinct clades or genotypes. Certain of these genotypes appeared to be geographically restricted while others were distributed widely throughout the region. Similarly, the complete nucleotide sequences of the RNA segment 10 (822 bp), coding for the non-structural protein (NS3/3A), were also conserved and grouped into the five genotypes; the BTV isolates could be grouped into three Asian genotypes and two Nth American/Sth African genotypes.
    MeSH terms: Amino Acid Sequence; Animals; Asia, Southeastern/epidemiology; Bluetongue/epidemiology; Bluetongue/virology*; Bluetongue virus/genetics*; Bluetongue virus/isolation & purification; Cattle; Cattle Diseases/epidemiology; Cattle Diseases/virology*; Genotype; Molecular Sequence Data; Phylogeny; RNA, Viral/isolation & purification; Serotyping; Genetic Variation*; Viral Core Proteins/genetics; Viral Nonstructural Proteins/genetics; Viral Nonstructural Proteins/chemistry; Molecular Epidemiology; Sentinel Surveillance; Evolution, Molecular; Reverse Transcriptase Polymerase Chain Reaction
  12. Tan WS, Ong ST, Eshaghi M, Foo SS, Yusoff K
    J Med Virol, 2004 May;73(1):105-12.
    PMID: 15042656
    The nucleocapsid (N) protein of Nipah virus (NiV) can be produced in three Escherichia coli strains [TOP10, BL21(DE3) and SG935] under the control of trc promoter. However, most of the product existed in the form of insoluble inclusion bodies. There was no improvement in the solubility of the product when this protein was placed under the control of T7 promoter. However, the solubility of the N protein was significantly improved by lowering the growth temperature of E. coli BL21(DE3) cell cultures. Solubility analysis of N- and C-terminally deleted mutants revealed that the full-length N protein has the highest solubility. The soluble N protein could be purified efficiently by sucrose gradient centrifugation and nickel affinity chromatography. Electron microscopic analysis of the purified product revealed that the N protein assembled into herringbone-like particles of different lengths. The C-terminal end of the N protein contains the major antigenic region when probed with antisera from humans and pigs infected naturally.
    MeSH terms: Animals; Antigens, Viral; Base Sequence; DNA, Viral/genetics; Escherichia coli/genetics; Humans; Microscopy, Electron; Rabbits; Recombinant Proteins/genetics; Recombinant Proteins/immunology; Recombinant Proteins/chemistry; Solubility; Sequence Deletion; Nucleocapsid Proteins/genetics; Nucleocapsid Proteins/immunology*; Nucleocapsid Proteins/chemistry*; Nipah Virus/genetics; Nipah Virus/immunology*; Nipah Virus/chemistry*; Henipavirus Infections/immunology; Henipavirus Infections/virology
  13. Chan YM, Goh KL
    Gastrointest Endosc, 2004 Apr;59(4):517-24.
    PMID: 15044888
    BACKGROUND: The appropriateness has become an important issue as the use of EGD becomes more widespread. No study of the appropriateness of EGD has been performed in the Asia-Pacific region. This study examined the appropriateness of EGD in a large Asian hospital by using the American Society for Gastrointestinal Endoscopy 2000 guidelines. A secondary aim was to correlate appropriateness with the presence of positive findings at EGD.

    METHODS: A cross-sectional study was conducted of consecutive diagnostic EGDs performed at a university-affiliated, teaching hospital, which has an open-access endoscopy system for doctors who work in the hospital. The main indication(s) for EGD was recorded and assessed as appropriate or inappropriate by using American Society for Gastrointestinal Endoscopy criteria. EGD findings were recorded and classified as positive or negative. Referrals were categorized as being from endoscopists, primary care physicians, and others.

    RESULTS: Of 1076 referrals for EGD, 88.3% were deemed appropriate. The group with the highest rate of appropriate referral was endoscopists (90.2%), followed by primary care physicians (89.6%) and "others" (81.9%). The rate of appropriate referrals was significantly higher for endoscopists and primary care physicians compared with "others" (respectively, p=0.001 and p=0.022). The most common appropriate indication was "upper abdominal distress that persists despite an appropriate trial of therapy" (35.4%). The most common inappropriate indication was "dyspepsia in patients aged 45 years or below without adequate empirical medical treatment" (48.4%); 42.2% with an appropriate indication had positive findings compared with only 25.6% of those with inappropriate indications (p=0.006). On multivariate analysis, the following were identified as independent predictive factors for positive findings at EGD: male gender (p=0.005), age over 45 years (p=0.011), smoking (p=0.005), none/primary education (p<0.001), and secondary education (p=0.026).

    CONCLUSIONS: The proportion of patients referred for open-access EGD with an appropriate indication(s) was high for all doctor groups in a large university-affiliated medical center in Asia. EGDs performed for appropriate indications had a higher yield of positive findings. Independent predictive factors of positive findings were male gender, age over 45 years, lower education level, and referral by an endoscopist.

    MeSH terms: Adult; Cross-Sectional Studies; Female; Hospitals, University; Humans; Malaysia; Male; Middle Aged; Prospective Studies; Referral and Consultation/statistics & numerical data; Multivariate Analysis; Endoscopy, Gastrointestinal/statistics & numerical data*
  14. Kiew LV, Munavvar AS, Law CH, Azizan AN, Nazarina AR, Sidik K, et al.
    J Physiol, 2004 Jun 15;557(Pt 3):981-9.
    PMID: 15047774
    An antisense oligodeoxynucleotide (As-ODN) to the 3' untranslated region of the mRNA sequence expressing the intracellular adhesion molecule-1 (ICAM-1) was employed to determine ICAM-1's role in renal ischaemia-reperfusion injury in the rat. Wistar-Kyoto rats receiving i.v. either lipofectin-As-ODN (As-ODN group), lipofectin-reverse ODN (Rv-ODN group) or lipofectin (ischaemia control group) 8 h prior to study were anaesthetized and subjected to 30 min of renal artery occlusion. Renal haemodynamic and excretory parameters were monitored before and after renal ischaemia. On termination of the study renal tissue was subjected to histological and Western blot analysis. Renal blood flow decreased in the 3 h post-ischaemia period in the ischaemia control and Rv-ODN groups, but was maintained in the As-ODN group. Glomerular filtration rate was depressed initially but gradually increased to 10% above basal levels in the ischaemia control and Rv-ODN groups, but was below basal levels (20%) in the As-ODN group. There was a three- to fourfold increase in sodium and water excretion following ischaemia in the ischaemia control and reverse-ODN groups but not in the As-ODN treated group. The As-ODN ameliorated the histological evidence of ischaemic damage and reduced ICAM-1 protein levels to a greater extent in the medulla than cortex. These observations suggested that in the post-ischaemic period afferent and efferent arteriolar tone was increased with a loss of reabsorptive capacity which was in part due to ICAM-1. The possibility arises that the action of ICAM-1 at vascular and tubular sites in the deeper regions of the kidney contributes to the ischaemia-reperfusion injury.
    MeSH terms: Anesthesia; Animals; Blood Pressure/drug effects; Kidney Diseases/drug therapy*; Kidney Diseases/pathology; Kidney Diseases/physiopathology; Male; Rats, Inbred WKY; Renal Circulation/drug effects; Urodynamics/drug effects; Water-Electrolyte Balance/drug effects; Blotting, Western; Reperfusion Injury/drug therapy*; Reperfusion Injury/pathology; Reperfusion Injury/physiopathology; Oligonucleotides, Antisense/therapeutic use*; Intercellular Adhesion Molecule-1/genetics*; Rats
  15. Puad HA, Noor MY
    J Environ Radioact, 2004;73(3):289-305.
    PMID: 15050361
    Crude oil terminal sludge contains technologically enhanced naturally occurring radionuclides such as (232)Th, (238)U, (228)Ra and (226)Ra, thus cannot be disposed of freely without proper control. The current method of disposal, such as land farming and storing in plastic drums is not recommended because it will have a long-term impact on the environment. Due to its organic nature, there is a move to treat this sludge by thermal methods such as incineration. This study has been carried out to determine the behaviors of (232)Th, (238)U, (228)Ra and (226)Ra present in the sludge during combustion at a certain temperature and time. The percentage of volatilization was found to vary between 2% and 70%, (238)U was the most volatile in comparison with (232)Th, (228)Ra and (226)Ra. (238)U is found to be significantly volatilized above 500 degrees C, and might reach maximum volatilization at above 700 degrees C. A mathematical model was developed to predict the percentage of volatilization of (232)Th, (238)U, (228)Ra and (226)Ra contained in the sludge. With this known percentage of volatilization, the concentration of (232)Th, (238)U, (228)Ra and (226)Ra present in the bottom and filter ashes can be calculated.
    MeSH terms: Air Pollutants, Radioactive/analysis*; Filtration; Petroleum*; Radioisotopes/analysis*; Refuse Disposal*; Ships; Volatilization; Incineration
  16. Radzi bin Abas M, Oros DR, Simoneit BR
    Chemosphere, 2004 May;55(8):1089-95.
    PMID: 15050806
    The haze episodes that occurred in Malaysia in September-October 1991, August-October 1994 and September-October 1997 have been attributed to suspended smoke particulate matter from biomass burning in southern Sumatra and Kalimantan, Indonesia. In the present study, polar organic compounds in aerosol particulate matter from Malaysia are converted to their trimethylsilyl derivatives and analyzed by gas chromatography-mass spectrometry in order to better assess the contribution of the biomass burning component during the haze episodes. On the basis of this analysis, levoglucosan was found to be the most abundant organic compound detected in almost all samples. The monosaccharides, alpha- and beta-mannose, the lignin breakdown products, vanillic and syringic acids and the minor steroids, cholesterol and beta-sitosterol were also present in some samples. The presence of the tracers from smoke overwhelmed the typical signatures of emissions from traffic and other anthropogenic activities in the urban areas.
    MeSH terms: Air Pollution/analysis*; Cholesterol; Gallic Acid/analogs & derivatives*; Glucose/analogs & derivatives*; Malaysia; Mannose; Gas Chromatography-Mass Spectrometry; Sitosterols; Smoke*; Trimethylsilyl Compounds/chemistry*; Vanillic Acid; Incineration*; Biomass*
  17. Singh B, Kim Sung L, Matusop A, Radhakrishnan A, Shamsul SS, Cox-Singh J, et al.
    Lancet, 2004 Mar 27;363(9414):1017-24.
    PMID: 15051281
    About a fifth of malaria cases in 1999 for the Kapit division of Malaysian Borneo had routinely been identified by microscopy as Plasmodium malariae, although these infections appeared atypical and a nested PCR assay failed to identify P malariae DNA. We aimed to investigate whether such infections could be attributable to a variant form of P malariae or a newly emergent Plasmodium species.
    MeSH terms: Adult; Animals; Chloroquine/therapeutic use; Erythrocytes/parasitology; Humans; Macaca/parasitology; Malaria/epidemiology; Malaria/parasitology*; Malaria/transmission*; Malaysia/epidemiology; Monkey Diseases/parasitology; Monkey Diseases/transmission; Plasmodium malariae/genetics; Plasmodium malariae/isolation & purification; Primaquine/therapeutic use; Zoonoses/epidemiology; Zoonoses/parasitology; Zoonoses/transmission*; DNA, Protozoan/isolation & purification; Polymerase Chain Reaction; Plasmodium knowlesi/genetics; Plasmodium knowlesi/isolation & purification*
  18. Lee WS
    Pediatr Int, 2004 Apr;46(2):198-201.
    PMID: 15056252
    MeSH terms: Infliximab; Antibodies, Monoclonal/therapeutic use*; Asia, Southeastern; Child; Child, Preschool; Crohn Disease/drug therapy*; Female; Gastrointestinal Agents/therapeutic use*; Humans; Male
  19. Shimizu H, Utama A, Onnimala N, Li C, Li-Bi Z, Yu-Jie M, et al.
    Pediatr Int, 2004 Apr;46(2):231-5.
    PMID: 15056257
    Recently, there have been large outbreaks of hand, foot and mouth disease (HFMD) mainly caused by enterovirus 71 (EV71) associated with severe neurological diseases in the Western Pacific Region (WPR). To monitor the realtime trend of EV71 transmission throughout the WPR, the authors conducted a molecular epidemiological analysis of EV71 infection.
    MeSH terms: China/epidemiology; Enterovirus/genetics*; Hand, Foot and Mouth Disease/epidemiology*; Hand, Foot and Mouth Disease/virology; Humans; Thailand/epidemiology
  20. Sie MY, Goh PK, Chan L, Ong SY
    Anaesth Intensive Care, 2004 Feb;32(1):28-30.
    PMID: 15058117
    This randomized controlled trial compared Bispectral Index (BIS) values in 40 patients after a modified rapid sequence induction using thiopentone 4 mg/kg or propofol 2 mg/kg with rocuronium 0.6 mg/kg as muscle relaxant. Endotracheal intubation was performed at 60 seconds from induction of anaesthesia and BIS values were recorded for three minutes after induction. At the 120, 150 and 180 second measurements there was a significantly greater proportion of subjects with BIS values < or = 60 ("anaesthetized") in the propofol group compared with the thiopentone group (P values < 0.02, < 0.01 and < 0.01 respectively). All intubations were completed within two minutes. No explicit recall of intubation was detected clinically with either induction agent. The BIS scores we have measured suggest that thiopentone 4 mg/kg is more likely to be associated with lighter planes of anaesthesia and consequent risk of awareness than propofol 2 mg/kg, if intubation is delayed or prolonged.
    MeSH terms: Adult; Androstanols/pharmacology*; Awareness/drug effects; Neuromuscular Nondepolarizing Agents/pharmacology*; Electroencephalography/drug effects*; Humans; Intubation, Intratracheal; Middle Aged; Thiopental/pharmacology*; Propofol/pharmacology*; Elective Surgical Procedures; Anesthetics, Intravenous/pharmacology*
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