Browse publications by year: 2006

  1. Han GS, Davies C
    Ethn Health, 2006 Nov;11(4):409-30.
    PMID: 17060035 DOI: 10.1080/13557850600824054
    This paper investigates the use and provision of biomedicine among Korean-Australian men on the basis of interview data from all of the eight Korean-speaking doctors practising in the Korean community in Sydney in 1995. From the viewpoint of these general practitioners, an analysis is made of the processes Korean men go through in adjusting to a new country, being involved in constant hard manual work and long working hours, and explores how they make use of all available resources to stay healthy. The Korean men have fully utilized the 'freely' available medical services under government-subsidized Medicare, bearing in mind that health is a capacity to work under the current environment, although illegal migrants restrained themselves from using it until they obtained legal status. Korean-speaking medical practitioners have been able to provide their fellow Koreans with 'culturally appropriate' health care, with the key factor being the absence of a language barrier. The level of patient satisfaction is high, possibly due to the excellent understanding the doctors have of the social aspects of illnesses, although the doctors do not go beyond curative medicine in their practice. However, the increasing number of Korean-speaking doctors in the small Korean community means that there is competition for patients. Consequently, the medical care is highly entrepreneurial. Referral by Korean doctors to practitioners of Korean herbal medicine is also a notable feature of the health care sector of the Korean community, especially as this offers Korean patients 'satisfactory' health relief for problems that are not easily relieved by doctors in the biomedical system.
    MeSH terms: Acculturation; Community Health Services/utilization*; Culture*; Emigration and Immigration*; Family Practice*; Humans; Interviews as Topic; Korea/ethnology; Male; New South Wales/ethnology; Physicians, Family/psychology*; Residence Characteristics; Entrepreneurship; Patient Satisfaction/ethnology*; Asian Continental Ancestry Group/ethnology*; Asian Continental Ancestry Group/psychology
  2. Khairul MF, Min TH, Low JH, Nasriyyah CH, A'shikin AN, Norazmi MN, et al.
    Jpn J Infect Dis, 2006 Oct;59(5):329-31.
    PMID: 17060702
    Fluoxetine (FLX), a P-glycoprotein inhibitor with antimalarial activity, is promising candidate for reversing chloroquine/mefloquine (CQ/MQ) resistance. The Dd2 strain of CQ- and MQ-resistant Plasmodium falciparum was synchronized and assayed with various concentrations of CQ/MQ individually and in combination with FLX or verapamil (VPL). Our results indicated the 50% inhibitory concentration values of CQ and MQ were greatly lowered when FLX was used simultaneously. Isobolograms indicated that CQ-FLX combinations are more synergistic (mean fractional inhibitory concentration [FIC] index 0.55) than MQ-FLX (mean FIC index 0.64), and their synergistic effect was better than or at par with CQ-VPL (mean FIC index 0.88) and MQ-VPL (mean FIC index 0.60) combinations. We conclude that the FLX potentiates the CQ and MQ response on multidrug-resistant P. falciparum at clinically achievable concentrations.
    MeSH terms: Animals; Antimalarials/pharmacology*; Cells, Cultured; Chloroquine/pharmacology*; Drug Synergism; Erythrocytes/parasitology; Fluoxetine/pharmacology*; Humans; Plasmodium falciparum/drug effects*; Plasmodium falciparum/isolation & purification; Mefloquine/pharmacology*; Malaria, Falciparum/parasitology*; Drug Resistance, Multiple; Inhibitory Concentration 50
  3. Rimmelzwaan GF, de Jong JC, Donker GA, Meijer A, Fouchier RA, Osterhaus AD
    Ned Tijdschr Geneeskd, 2006 Oct 7;150(40):2209-14.
    PMID: 17061434
    The first sign of influenza activity in the Netherlands during the 2005-2006 influenza season was the isolation of influenza viruses in the last week of 2005. From Week 1 of 2006 onwards, an increase in clinical influenza activity was also observed that did not return to baseline levels until Week 15. Two waves of influenza activity were observed with peak incidences of 13.8 and 9.8 influenza-like illnesses per 10,000 inhabitants on Weeks 7 and 12, respectively. The first wave of influenza was caused primarily by influenza B viruses, whereas the second wave was caused predominantly by influenza A/H3N2 viruses. The influenza B viruses appeared to belong to two different phylogenetic lineages and were antigenically distinguishable from the vaccine strain. The isolated influenza A/H3N2 viruses were closely related to the vaccine strain for this subtype and only minor antigenic differences with the vaccine strain were observed for a limited number of isolates. Only a small number of influenza A/H1N1 viruses were isolated, which all closely resembled the H1N1 vaccine strain. For the 2006-2007 influenza season, the World Health Organization has recommended the following vaccine composition: A/Wisconsin/67/05 (H3N2), A/New Caledonia/20/99 (H1N1) and B/Malaysia/2506/05.
    MeSH terms: Disease Outbreaks/prevention & control*; Forecasting; Humans; Influenza, Human/prevention & control*; Influenza, Human/virology; Netherlands; Influenza B virus/immunology*; Influenza B virus/isolation & purification; Seasons; Global Health; Influenza A Virus, H1N1 Subtype/immunology*; Influenza A Virus, H1N1 Subtype/isolation & purification; Influenza A Virus, H3N2 Subtype/immunology*; Influenza A Virus, H3N2 Subtype/isolation & purification
  4. Sosroseno W, Musa M, Ravichandran M, Fikri Ibrahim M, Bird PS, Seymour GJ
    Oral Microbiol. Immunol., 2006 Dec;21(6):347-52.
    PMID: 17064391
    The aim of the present study was to determine the role of cyclic adenosine monophosphate (cAMP) on arginase activity in a murine macrophage cell line (RAW264.7 cells) stimulated with lipopolysaccharide (LPS) from Actinobacillus actinomycetemcomitans.
    MeSH terms: Adenylyl Cyclase Inhibitors; Cyclic AMP/physiology*; Animals; Arginase/antagonists & inhibitors; Arginase/metabolism*; Cell Line; Cycloheximide/pharmacology; Enzyme Activation; Colforsin/pharmacology; Lipopolysaccharides/pharmacology; Macrophages/drug effects; Macrophages/enzymology*; Phosphodiesterase Inhibitors/pharmacology; Protein Synthesis Inhibitors/pharmacology; 1-Methyl-3-isobutylxanthine/pharmacology; Aggregatibacter actinomycetemcomitans/chemistry*; Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors; Cyclic AMP-Dependent Protein Kinases/metabolism*; Protein Kinase Inhibitors/pharmacology; Mice
  5. Sosroseno W, Bird PS, Gemmell E, Seymour GJ
    Oral Microbiol. Immunol., 2006 Dec;21(6):411-4.
    PMID: 17064401
    The aim of this study was to determine the role of CD4 and CD8 cells on specific antibody production by murine Peyer's patch (PP) cells after oral immunization with Actinomyces viscosus in mice. Female DBA/2 mice were orally immunized with three low doses of heat-killed A. viscosus. Sham-immunized mice served as a control group. Mice were depleted of CD4 or CD8 cells by intraperitoneal injection of anti-CD4 or anti-CD8 antibodies daily for 3 days before oral immunization. One week after the last oral immunization, PPs were removed and cell suspensions were cultured with A. viscosus. Specific antibody production in the culture supernatants was assessed by enzyme-linked immunosorbent assay. The results showed that oral immunization with A. viscosus induced a predominant specific immunoglobulin A (IgA) response by PP cells and, to a lesser extent, IgM antibodies. Depletion of CD4 but not CD8 cells suppressed the production of specific antibodies. These results suggest that oral immunization with low doses of A. viscosus may induce the production of specific antibodies by murine PP cells in a CD4-cell-dependent fashion.
    MeSH terms: Animals; Antibodies, Bacterial/biosynthesis*; Female; Immunoglobulin A/biosynthesis; Immunoglobulin M/biosynthesis; Mice, Inbred DBA; Peyer's Patches/immunology*; CD4-Positive T-Lymphocytes/physiology*; Actinomyces viscosus/immunology*; CD8-Positive T-Lymphocytes/physiology*; Immunity, Mucosal; Mice
  6. Lang G, Mitova MI, Cole AL, Din LB, Vikineswary S, Abdullah N, et al.
    J Nat Prod, 2006 Oct;69(10):1389-93.
    PMID: 17067148
    Six new linear peptides, pterulamides I-VI (1-6), were isolated from the fruiting bodies of a Malaysian Pterula species. The structures were elucidated by MS and 2D NMR experiments, and the absolute configurations of the constituent amino acids established using Marfey's method. The pterulamides are mainly assembled from nonpolar N-methylated amino acids and, most interestingly, have non-amino-acid N-terminal groups, among them the unusual cinnamoyl, (E)-3-methylsulfinylpropenoyl, and (E)-3-methylthiopropenoyl groups. Furthermore, pterulamides I-V are the first natural peptides with a methylamide C-terminus. Pterulamides I and IV are cytotoxic against the P388 cell line with IC50 values of 0.55 and 0.95 microg/mL (0.79 and 1.33 microM), respectively.
    MeSH terms: Animals; Basidiomycota/chemistry*; Drug Screening Assays, Antitumor; Leukemia P388; Malaysia; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Mice
  7. Jamal N, Ng KH, Looi LM, McLean D, Zulfiqar A, Tan SP, et al.
    Phys Med Biol, 2006 Nov 21;51(22):5843-57.
    PMID: 17068368
    We describe a semi-automated technique for the quantitative assessment of breast density from digitized mammograms in comparison with patterns suggested by Tabar. It was developed using the MATLAB-based graphical user interface applications. It is based on an interactive thresholding method, after a short automated method that shows the fibroglandular tissue area, breast area and breast density each time new thresholds are placed on the image. The breast density is taken as a percentage of the fibroglandular tissue to the breast tissue areas. It was tested in four different ways, namely by examining: (i) correlation of the quantitative assessment results with subjective classification, (ii) classification performance using the quantitative assessment technique, (iii) interobserver agreement and (iv) intraobserver agreement. The results of the quantitative assessment correlated well (r2 = 0.92) with the subjective Tabar patterns classified by the radiologist (correctly classified 83% of digitized mammograms). The average kappa coefficient for the agreement between the readers was 0.63. This indicated moderate agreement between the three observers in classifying breast density using the quantitative assessment technique. The kappa coefficient of 0.75 for intraobserver agreement reflected good agreement between two sets of readings. The technique may be useful as a supplement to the radiologist's assessment in classifying mammograms into Tabar's pattern associated with breast cancer risk.
    MeSH terms: Algorithms; Artificial Intelligence; Breast Neoplasms/diagnosis*; Female; Humans; Mammography/methods*; Pattern Recognition, Automated/methods; Prognosis; Radiographic Image Enhancement/methods*; Radiographic Image Interpretation, Computer-Assisted/methods*; Risk Factors; Sensitivity and Specificity; Software; Reproducibility of Results; Absorptiometry, Photon/methods*; Risk Assessment/methods*
  8. Lee SM, Wong WP, Hiong KC, Loong AM, Chew SF, Ip YK
    J. Exp. Zoolog. Part A Comp. Exp. Biol., 2006 Dec 1;305(12):995-1009.
    PMID: 17068799
    This study aimed to determine effects of 6-day progressive increase in salinity from 1 per thousand to 15 per thousand on nitrogen metabolism and excretion in the soft-shelled turtle, Pelodiscus sinensis. For turtles exposed to 15 per thousand water on day 6, the plasma osmolality and concentrations of Na+, Cl- and urea increased significantly, which presumably decreased the osmotic loss of water. Simultaneously, there were significant increases in contents of urea, certain free amino acids (FAAs) and water-soluble proteins that were involved in cell volume regulation in various tissues. There was an apparent increase in proteolysis, releasing FAAs as osmolytes. In addition, there might be an increase in catabolism of certain amino acids, producing more ammonia. The excess ammonia was retained as indicated by a significant decrease in the rate of ammonia excretion on day 4 in 15 per thousand water, and a major portion of it was converted to urea. The rate of urea synthesis increased 1.4-fold during the 6-day period, although the capacity of the hepatic ornithine urea cycle remained unchanged. Urea was retained for osmoregulation because there was a significant decrease in urea excretion on day 4. Increased protein degradation and urea synthesis implies greater metabolic demands, and indeed turtles exposed to 15 per thousand water had significantly higher O2 consumption rate than the freshwater (FW) control. When turtles were returned from 15 per thousand water to FW on day 7, there were significant increases in ammonia (probably released through increased amino acid catabolism) and urea excretion, confirming that FAAs and urea were retained for osmoregulatory purposes in brackish water.
    MeSH terms: Amino Acids/blood; Ammonia/metabolism; Animals; Blood Chemical Analysis; Chlorides/metabolism; Hematocrit; Liver/metabolism; Malaysia; Nitrogen/metabolism*; Osmolar Concentration; Oxygen Consumption/physiology; Seawater/chemistry*; Sodium Chloride/analysis*; Turtles/metabolism*; Urea/metabolism; Water-Electrolyte Balance/physiology*; Muscle, Skeletal/metabolism
  9. Tan EH, Batchelor P, Sheiham A
    Int Dent J, 2006 Oct;56(5):277-82.
    PMID: 17069070
    OBJECTIVES: To reassess the recall frequency interval for dental examinations for children, based on annual caries increments.

    METHODS: Cross sectional data collected on school children in eight rural and urban schools through the national Incremental Dental Care Programme (IDCP) for one district in Malaysia were analysed to assess their annual caries increment and trend lines. The Restorative Index was calculated to assess the success of the IDCP in rendering children dentally fit.

    RESULTS: The annual caries increments were low; the current caries levels were between 0.65 and 1.50 for 12 year-old children in Kota Tinggi District. Most of the caries experience was on pits and fissures. From 7 to 12 years old, the overall annual caries increment for the total study population was 0.19. The mean annual caries increment increased slightly between the ages of 12 to 14 years and 14 to 16 years and was 0.24 and 0.25 respectively. Two distinct caries incremental trend lines were observed for children aged 7 to 16 years. One group reached a mean DMFT of about 0.75 while the other group a mean DMFT of about 1.4 at 12 years. The trend lines continued over the next 4 years until the children were 16 years old. The Restorative Index was higher in urban schools that also had low DMFT levels.

    CONCLUSIONS: Based on the low annual caries increments of between 0.65 and 1.50, yearly dental examination intervals can safely be extended to 2-yearly intervals or even longer. Such a change of screening recall intervals would help improve resource allocation. Resources saved by extending recall intervals can be redirected to the small proportion of children with higher disease levels. This will help render more school children dentally fit and reduce inequalities in oral health.

    MeSH terms: Adolescent; Child; Cross-Sectional Studies; Dental Care/organization & administration*; Dental Caries/epidemiology*; Dental Caries/prevention & control*; DMF Index; Humans; Malaysia/epidemiology; Mass Screening; Incidence; Reminder Systems; Age Distribution
  10. Komabayashi T, Kawamura M, Kim KJ, Wright FA, Declerck D, Goiâs Mdo C, et al.
    Int Dent J, 2006 Oct;56(5):310-6.
    PMID: 17069075
    OBJECTIVE: To explore and describe international oral health attitudes/ behaviours among final year dental students.

    METHODS: Validated translated versions of the Hiroshima University-Dental Behavioural Inventory (HU-DBI) questionnaire were administered to 1,096 final-year dental students in 17 countries. Hierarchical cluster analysis was conducted within the data to detect patterns and groupings.

    RESULTS: The overall response rate was 72%. The cluster analysis identified two main groups among the countries. Group 1 consisted of twelve countries: one Oceanic (Australia), one Middle-Eastern (Israel), seven European (Northern Ireland, England, Finland, Greece, Germany, Italy, and France) and three Asian (Korea, Thailand and Malaysia) countries. Group 2 consisted of five countries: one South American (Brazil), one European (Belgium) and three Asian (China, Indonesia and Japan) countries. The percentages of 'agree' responses in three HU-DBI questionnaire items were significantly higher in Group 2 than in Group 1. They include: "I worry about the colour of my teeth."; "I have noticed some white sticky deposits on my teeth."; and "I am bothered by the colour of my gums."

    CONCLUSION: Grouping the countries into international clusters yielded useful information for dentistry and dental education.

    MeSH terms: Asia; Attitude to Health/ethnology*; Brazil; Cross-Cultural Comparison; Dental Care/psychology*; Europe; Humans; Oral Health*; Oral Hygiene/psychology*; Surveys and Questionnaires; Students, Dental/psychology; Health Behavior/ethnology*; Cluster Analysis
  11. Low BY, Liong ML, Yuen KH, Chong WL, Chee C, Leong WS, et al.
    Urology, 2006 Oct;68(4):751-8.
    PMID: 17070347
    To determine the prevalence, severity, and quality-of-life (QOL) impact of female lower urinary tract symptoms (FLUTS); to determine the patterns, reasons, and factors contributing to the women's treatment-seeking behavior; and to describe the relationship between the social demographic characteristics and FLUTS.
    MeSH terms: Adult; Female; Humans; Malaysia/epidemiology; Patient Acceptance of Health Care*; Quality of Life; Risk Factors; Severity of Illness Index; Urination Disorders/epidemiology*; Urination Disorders/psychology; Urination Disorders/therapy*; Prevalence*; Health Care Surveys
  12. Tay ST, Lim HC, Tajuddin TH, Rohani MY, Hamimah H, Thong KL
    Med Mycol, 2006 Nov;44(7):617-22.
    PMID: 17071555
    The molecular types and genetic heterogeneity of Cryptococcus neoformans and C. gattii clinical isolates in Malaysia were determined in this study. Of 44 C. neoformans collected between 1980 and 2003, 42 (95.5%) were molecular type VNI, 2 (4.5%) were molecular type VNII. Of 17 C.gattii isolates, 13 (76.5%) were molecular type VGI, and 4 (23.5%) were molecular type VGII. A difference was noted when comparing the molecular types of cryptococcal isolates in the earlier and recent cases of cryptococcosis. While both molecular types VNI and VGI were equally predominant in the earlier cases of cryptococcosis, VNI was the most predominant molecular type isolated from the recent cases. VNII was a new molecular type, isolated from 5.1% of the recent cases. All the bird dropping isolates were molecular type VNI. The genetic heterogeneity of the two predominant molecular types, i.e., VNI, VGI clinical isolates and bird dropping isolates of C. neoformans were further determined by polymerase chain reaction (PCR) fingerprinting method, using (GTG)5 as single primer. Two clusters of cryptococcal isolates were distinguished at 68.5% of similarity, with cluster I consisting of VNI isolates and cluster II consisting of VGI isolates. Each cluster was further subdivided into three subtypes at >/=80% of similarity. Fourteen bird dropping isolates were grouped into a subtype within VN1, sharing 82.7% of similarity with the clinical isolates. A higher degree of similarities, ranging from 93.4-97.6% was noted between 3 bird dropping isolates with the clinical isolates in another subtype. This study demonstrated the existence of various molecular types of C. neoformans isolates in Malaysia and the genetic heterogeneity within the predominant molecular types. The study also provides evidence for genetic relatedness of clinical isolates with bird dropping isolates in the environment.
    MeSH terms: Cryptococcosis/microbiology; Cryptococcosis/epidemiology*; Cryptococcus neoformans/genetics; Cryptococcus neoformans/isolation & purification*; Humans; Malaysia/epidemiology; Phylogeny; Mycological Typing Techniques; Molecular Epidemiology; Genetic Heterogeneity*
  13. Soda W, Noble AD, Suzuki S, Simmons R, Sindhusen LA, Bhuthorndharaj S
    J Environ Qual, 2006 Oct 27;35(6):2293-301.
    PMID: 17071900
    Acid waste bentonite is a byproduct from vegetable oil bleaching that is acidic (pH < 3.0) and hydrophobic. These materials are currently disposed of in landfills and could potentially have a negative impact on the effective function of microbes that are intolerant of acidic conditions. A study was undertaken using three different sources of acid waste bentonites, namely soybean oil bentonite (SB), palm oil bentonite (PB), and rice bran oil bentonite (RB). These materials were co-composted with rice husk, rice husk ash, and chicken litter to eliminate their acid reactivity and hydrophobic nature. The organic carbon (OC) content, pH, exchangeable cations, and cation exchange capacity (CEC) of the acid-activated bentonites increased significantly after the co-composting phase. In addition, the hydrophobic nature of these materials as measured using the water drop penetration time (WDPT) decreased from >10 800 s to 16 to 80 s after composting. Furthermore, these composted materials showed positive impacts on soil physical attributes including specific surface area, bulk density, and available water content for crop growth. Highly significant increases in maize biomass (Zea mays L.) production over two consecutive cropping cycles was observed in treatments receiving co-composted bentonite. The study clearly demonstrates the potential for converting an environmentally hazardous material into a high-quality soil conditioner using readily available agricultural byproducts. It is envisaged that the application of these composted acid waste bentonites to degraded soils will increase productivity and on-farm income, thus contributing toward food security and poverty alleviation.
    MeSH terms: Bentonite/toxicity*; Bentonite/chemistry; Carbon/metabolism; Carbon/chemistry; Chromatography, Ion Exchange; Environmental Monitoring*; Humidity; Hydrogen-Ion Concentration; Organic Chemicals/metabolism; Organic Chemicals/chemistry; Refuse Disposal*; Soil Pollutants/toxicity*; Soil Pollutants/chemistry; Time Factors; Biomass; Crops, Agricultural/drug effects*; Crops, Agricultural/growth & development
  14. Yee HY, Radhakrishnan A, Ponnudurai G
    Med Teach, 2006 Sep;28(6):558-60.
    PMID: 17074705
    Students' perception of the role and characteristics of a good problem-based learning (PBL) facilitator were assessed in the same study in which students were exposed to the 'Flying a Kite Approach' to PBL. A pre-tested anonymous questionnaire addressed the good qualities of a facilitator as well as the negative aspects. Although faculty and students' perceptions of 'good 'and 'bad' attributes generally agreed, it is clear that students still prefer facilitators who talk more, i.e. explain unclear facts or correct them when their facts are wrong. Content experts are also preferred over non-content experts.
    MeSH terms: Evaluation Studies as Topic*; Faculty, Medical/standards*; Humans; Surveys and Questionnaires; Schools, Medical*; Students, Medical/psychology*; Problem-Based Learning*; Internationality*
  15. Smith GJ, Fan XH, Wang J, Li KS, Qin K, Zhang JX, et al.
    Proc Natl Acad Sci U S A, 2006 Nov 07;103(45):16936-41.
    PMID: 17075062
    The development of highly pathogenic avian H5N1 influenza viruses in poultry in Eurasia accompanied with the increase in human infection in 2006 suggests that the virus has not been effectively contained and that the pandemic threat persists. Updated virological and epidemiological findings from our market surveillance in southern China demonstrate that H5N1 influenza viruses continued to be panzootic in different types of poultry. Genetic and antigenic analyses revealed the emergence and predominance of a previously uncharacterized H5N1 virus sublineage (Fujian-like) in poultry since late 2005. Viruses from this sublineage gradually replaced those multiple regional distinct sublineages and caused recent human infection in China. These viruses have already transmitted to Hong Kong, Laos, Malaysia, and Thailand, resulting in a new transmission and outbreak wave in Southeast Asia. Serological studies suggest that H5N1 seroconversion in market poultry is low and that vaccination may have facilitated the selection of the Fujian-like sublineage. The predominance of this virus over a large geographical region within a short period directly challenges current disease control measures.
    MeSH terms: Animals; Antigenic Variation; Antigens, Viral/genetics; China/epidemiology; Influenza in Birds/epidemiology; Influenza in Birds/virology; Humans; Influenza, Human/epidemiology; Influenza, Human/virology; Molecular Sequence Data; Phylogeny; Poultry/virology; Selection, Genetic; Time Factors; Genetic Variation*; Molecular Epidemiology; Evolution, Molecular; Hemagglutinin Glycoproteins, Influenza Virus/genetics; Hemagglutinin Glycoproteins, Influenza Virus/immunology; Influenza A Virus, H5N1 Subtype/genetics*; Influenza A Virus, H5N1 Subtype/immunology; Influenza A Virus, H5N1 Subtype/isolation & purification; Influenza A Virus, H5N1 Subtype/pathogenicity
  16. Siti-Noor AS, Wan-Maziah WM, Narazah MY, Quah BS
    Singapore Med J, 2006 Nov;47(11):935-9.
    PMID: 17075659
    To determine the prevalence of iron deficiency (ID) and iron deficiency anaemia (IDA) in Kelantanese pre-school children and to identify risk factors that best predict the presence of ID.
    MeSH terms: Ambulatory Care Facilities; Child; Child, Preschool; Cross-Sectional Studies; Female; Humans; Infant; Malaysia/epidemiology; Male; Risk Factors; Prevalence; Logistic Models; Anemia, Iron-Deficiency/etiology*; Anemia, Iron-Deficiency/epidemiology
  17. Chua SK, Selvanesan S, Sivalingam B, Chem YK, Norizah I, Zuridah H, et al.
    Singapore Med J, 2006 Nov;47(11):940-6.
    PMID: 17075660
    During an outbreak from December 2004 to March 2005, 138 isolates of dengue virus were prospectively obtained from acute-phase serum samples of 1,067 patients with the provisional clinical diagnosis of acute dengue illness admitted to the adult wards of Hospital Tengku Ampuan Rahimah, Klang, Malaysia. Of the 138 dengue virus isolates, 87, 11, 24 and 3 were typed as dengue serotypes 1, 2, 3 and 4, respectively, by a commercial dengue virus typing kit using monoclonal antibodies (Mab). 13 dengue virus isolates could not be assigned to any specific serotype by serotyping Mab and molecular typing using dengue-type specific molecular typing primer pairs. We report the associated clinical features and limited molecular genetics of this Mab-escape dengue virus variant.
    MeSH terms: Amino Acid Sequence; Animals; Antibodies, Monoclonal*; Antibodies, Viral/blood; Cell Line; Dengue Virus/classification*; Dengue Virus/isolation & purification*; Disease Outbreaks; Humans; Malaysia/epidemiology; Molecular Sequence Data; Prospective Studies; RNA, Viral/analysis; Serotyping; Severe Dengue/epidemiology; Severe Dengue/virology; Reverse Transcriptase Polymerase Chain Reaction/methods*
  18. Wahab HA, Ahmad Khairudin NB, Samian MR, Najimudin N
    BMC Struct Biol, 2006;6:23.
    PMID: 17076907
    Polyhydroxyalkanoates (PHA), are biodegradable polyesters derived from many microorganisms such as the pseudomonads. These polyesters are in great demand especially in the packaging industries, the medical line as well as the paint industries. The enzyme responsible in catalyzing the formation of PHA is PHA synthase. Due to the limited structural information, its functional properties including catalysis are lacking. Therefore, this study seeks to investigate the structural properties as well as its catalytic mechanism by predicting the three-dimensional (3D) model of the Type II Pseudomonas sp. USM 4-55 PHA synthase 1 (PhaC1P.sp USM 4-55).
    MeSH terms: Acyltransferases/chemistry*; Amino Acid Sequence; Bacterial Proteins/chemistry*; Catalysis; Models, Molecular; Molecular Sequence Data; Pseudomonas/enzymology*; Sequence Alignment; Protein Structure, Secondary; Sequence Analysis, Protein; Structural Homology, Protein
  19. Zakaria ZA, Gopalan HK, Zainal H, Mohd Pojan NH, Morsid NA, Aris A, et al.
    Yakugaku Zasshi, 2006 Nov;126(11):1171-8.
    PMID: 17077618
    AIM: The present study was carried out to evaluate the antinociceptive, anti-inflammatory and antipyretic effects of chloroform extract of Solanum nigrum leaves using various animal models.

    METHODS: The extract was prepared by soaking (1:20; w/v) the air-dried powdered leaves (20 g) in chloroform for 72 hrs followed by evaporation (40 degrees C) under reduced pressure to dryness (1.26 g) and then dissolved (1:50; w/v) in dimethylsulfoxide (DMSO). The supernatant, considered as the stock solution with dose of 200 mg/kg, was diluted using DMSO to 20 and 100 mg/kg, and all doses were administered (s.c.; 10 ml/kg) in mice/rats 30 min prior to tests.

    RESULTS: The extract exhibited significant (p<0.05) antinociceptive activity when assessed using the abdominal constriction, hot plate and formalin tests. The extract also produced significant (p<0.05) anti-inflammatory and antipyretic activities when assessed using the carrageenan-induced paw edema and brewer's yeast-induced pyrexia tests. Overall, the activities occurred in a dose-independent manner.

    CONCLUSION: The present study demonstrated that the lipid-soluble extract of S. nigrum leaves possessed antinociceptive, anti-inflammatory and anti-pyretic properties and confirmed the traditional claims.

    MeSH terms: Animals; Chloroform*; Dimethyl Sulfoxide; Disease Models, Animal; Dose-Response Relationship, Drug; Edema/drug therapy*; Fever/drug therapy*; Inflammation/drug therapy*; Male; Phytotherapy*; Mice, Inbred BALB C; Pain/drug therapy*; Plant Extracts/isolation & purification; Plant Extracts/therapeutic use; Solutions; Rats, Sprague-Dawley; Plant Leaves/chemistry*; Solanum nigrum/chemistry*; Mice; Rats
  20. Zakaria ZA, Abdul Ghani ZD, Raden Mohd Nor RN, Gopalan HK, Sulaiman MR, Abdullah FC
    Yakugaku Zasshi, 2006 Nov;126(11):1197-203.
    PMID: 17077622
    The present study was carried out to establish the antinociceptive and anti-inflammatory properties of Dicranopteris linearis leaves chloroform extract in experimental animals. The antinociceptive activity was measured using the abdominal constriction, formalin and hot plate tests, while the anti-inflammatory activity was measured using the carrageenan-induced paw edema. The extract, obtained after 72 h soaking of the air-dried leaves in chloroform followed by evaporation under vacuo (40 degrees C) to dryness, was dissolved in dimethyl sulfoxide to the doses of 20, 100 and 200 mg/kg and administered subcutaneously 30 min prior to subjection to the above mentioned assays. The extract, at all doses used, was found to exhibit significant (p<0.05) antinociceptive activity in a dose-dependent manner. However, the significant (p<0.05) anti-inflammatory activity observed occur in a dose-independent manner. As a conclusion, the chloroform extract of D. linearis possesses antinociceptive and anti-inflammatory activity and thus justify its traditional uses by the Malays to treat various ailments.
    MeSH terms: Animals; Chloroform*; Dimethyl Sulfoxide; Disease Models, Animal; Dose-Response Relationship, Drug; Edema/drug therapy*; Fever/drug therapy*; Inflammation/drug therapy*; Male; Phytotherapy*; Mice, Inbred BALB C; Pain/drug therapy*; Plant Extracts/isolation & purification; Plant Extracts/therapeutic use; Plants, Medicinal/chemistry*; Solutions; Rats, Sprague-Dawley; Plant Leaves/chemistry*; Mice; Rats
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