Browse publications by year: 2010

  1. Al-Adhroey AH, Nor ZM, Al-Mekhlafi HM, Mahmud R
    Malar J, 2010 May 24;9:137.
    PMID: 20497543 DOI: 10.1186/1475-2875-9-137
    BACKGROUND: Despite continuous efforts by the government and private sectors, malaria is still a public health problem in rural Peninsular Malaysia. This study investigated household knowledge, attitude and practices (KAP) regarding malaria in two malaria endemic communities, forest-aboriginal and rural communities, in the Lipis district of Pahang state, Malaysia.

    METHODS: A descriptive cross-sectional study with a semi-structured questionnaire was carried out among 100 and 123 households from forest-aboriginal and rural areas, respectively.

    RESULTS: Knowledge about malaria and its transmission is significantly higher among the rural participants than the aborigines (86.2% vs 76%, p < 0.01). However, use of medicinal plants and beliefs in witchcraft and sorcery in treating febrile diseases were significantly higher among the aboriginal population (p < 0.01). There were no significant differences between the two communities in terms of the knowledge about malaria symptoms, attitudes towards its severity and practices in preventive measures against malaria by using mosquito bed nets. However, the knowledge and practice of different preventive measures to combat malaria, such as insecticide and the elimination of breeding areas, was significantly higher among the rural population than the aborigines (p < 0.001).

    CONCLUSIONS: Both communities were aware of malaria as a disease, but knowledge, attitudes and practices were inadequate. Providing efficient health education to people residing in malaria endemic areas would improve their understanding about malaria prevention in order to bring about the elimination of malaria from the country.

    MeSH terms: Adolescent; Adult; Cross-Sectional Studies; Cross-Cultural Comparison; Family Characteristics; Female; Humans; Health Knowledge, Attitudes, Practice*; Malaria/ethnology; Malaria/epidemiology; Malaria/prevention & control*; Malaria/transmission; Malaysia; Male; Mosquito Control/methods*; Patient Acceptance of Health Care*; Surveys and Questionnaires; Rural Population; Socioeconomic Factors; Health Behavior*; Young Adult
  2. Jalilian B, Omar AR, Bejo MH, Alitheen NB, Rasoli M, Matsumoto S
    PMID: 20497569 DOI: 10.1186/1479-0556-8-4
    Studies have shown that DNA vaccines can induce protective immunity, which demonstrated the high potential of DNA vaccines as an alternative to inactivated vaccines. Vaccines are frequently formulated with adjuvants to improve their release, delivery and presentation to the host immune system.
  3. Sarinah B, Hisham AN
    Asian J Surg, 2010 Jan;33(1):20-4.
    PMID: 20497878 DOI: 10.1016/S1015-9584(10)60004-8
    Primary thyroid lymphoma is uncommon and accounts for less than 2-5% of all thyroid malignancies. The aim of the present study was to review our experience and management of primary thyroid lymphoma and to discuss the diagnostic and therapeutic considerations.
    MeSH terms: Adult; Aged; Aged, 80 and over; Antineoplastic Combined Chemotherapy Protocols; Cyclophosphamide; Doxorubicin; Female; Humans; Lymphoma/diagnosis*; Lymphoma/pathology; Lymphoma/therapy*; Male; Middle Aged; Prednisolone; Radiotherapy; Thyroid Neoplasms/diagnosis*; Thyroid Neoplasms/pathology; Thyroid Neoplasms/therapy*; Thyroidectomy; Vincristine; Biopsy, Fine-Needle
  4. Lee HK, Ghani AR, Awang MS, Sayuthi S, Idris B, Abdullah JM
    Asian J Surg, 2010 Jan;33(1):42-50.
    PMID: 20497882 DOI: 10.1016/S1015-9584(10)60008-5
    Intracerebral haemorrhage (ICH) is the most disabling and least treatable form of stroke. Its risk factors include old age, hypertension, diabetes mellitus, hypercholesterolaemia, smoking and high alcohol intake, which are also associated with arterial stiffness. The aim of the present study was to determine the prognostic value of high augmentation index (AI), which is a surrogate marker of arterial stiffness, in patients with spontaneous ICH.
    MeSH terms: Aged; Cerebral Hemorrhage/diagnosis*; Diagnostic Techniques, Cardiovascular; Female; Health Status Indicators; Humans; Male; Middle Aged; Predictive Value of Tests; Prognosis; Prospective Studies; Risk Factors; Glasgow Coma Scale; Logistic Models
  5. Chan KM, Rajab NF, Siegel D, Din LB, Ross D, Inayat-Hussain SH
    Toxicol. Sci., 2010 Aug;116(2):533-48.
    PMID: 20498002 DOI: 10.1093/toxsci/kfq151
    Goniothalamin (GN), a styryl-lactone isolated from Goniothalamus andersonii, has been demonstrated to possess antirestenostic properties by inducing apoptosis on coronary artery smooth muscle cells (CASMCs). In this study, the molecular mechanisms of GN-induced CASMCs apoptosis were further elucidated. Apoptosis assessment based on the externalization of phosphatidylserine demonstrated that GN induces CASMCs apoptosis in a concentration-dependent manner. The GN-induced DNA damage occurred with concomitant elevation of p53 as early as 2 h, demonstrating an upstream signal for apoptosis. However, the p53 elevation in GN-treated CASMCs was independent of NAD(P)H: quinone oxidoreductase 1 and Mdm-2 expression. An increase in hydrogen peroxide and reduction in free thiols confirmed the role for oxidative stress in GN treatment. Pretreatment with the pan-caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (z-VAD-FMK) that significantly abrogated GN-induced CASMCs apoptosis suggested the involvement of caspase(s). The role of apical caspase-2, -8, and -9 was then investigated, and sequential activation of caspase-2 and -9 but not caspase-8 leading to downstream caspase-3 cleavage was observed in GN-treated CASMCs. Reduction of ATP level and decrease in oxygen consumption further confirmed the role of mitochondria in GN-induced apoptosis in CASMCs. The mitochondrial release of cytochrome c was seen without mitochondrial membrane potential loss and was independent of cardiolipin. These data provide insight into the mechanisms of GN-induced apoptosis, which may have important implications in the development of drug-eluting stents.
    MeSH terms: Adenosine Triphosphate/analysis; Amino Acid Chloromethyl Ketones/pharmacology; Cells, Cultured; Dose-Response Relationship, Drug; Enzyme Activation; Humans; Hydrogen Peroxide/metabolism; Muscle, Smooth, Vascular/drug effects*; Oxygen Consumption/drug effects; Pyrones/toxicity*; Superoxides/metabolism; Tumor Suppressor Protein p53/physiology*; NAD(P)H Dehydrogenase (Quinone)/metabolism; Apoptosis/drug effects*; Myocytes, Smooth Muscle/drug effects*; Cytochromes c/secretion; Membrane Potential, Mitochondrial/drug effects; Caspase 2/metabolism*
  6. Keat EC, Razak SS, Fadil NM, Yusof FM, Chan LH, Chyi FK, et al.
    Clin Ter, 2010;161(2):117-20.
    PMID: 20499023
    Piper betel (PB) possesses antimicrobial, antifungal, antioxidant and wound healing properties due to its powerful antioxidant effect. Diabetes mellitus (DM) is a metabolic disorder which is associated with complications like impaired wound healing, nephropathy and neuropathy. The main aim of the study was to study the wound healing properties of PB.
    MeSH terms: Animals; Antioxidants; Diabetes Mellitus, Experimental*; Male; Phytotherapy*; Plant Extracts/therapeutic use*; Wound Healing/drug effects*; Rats, Sprague-Dawley; Piper betle*; Rats
  7. Zahara AM, Lee CC, Fatimah IS, Poh BK, Khairul O, Das S, et al.
    Clin Ter, 2010;161(2):121-4.
    PMID: 20499024
    Intake of the antioxidant vitamins C and E lowers the oxidative stress. The study aimed to determine plasma concentrations of vitamin C and tocotrienols after supplementation of both vitamins in young male adults.
    MeSH terms: Adult; Ascorbic Acid/blood*; Chromans; Humans; Male; Vitamin E/analogs & derivatives; Vitamins/blood*; Dietary Supplements*; Tocotrienols/blood*; Young Adult
  8. Fatimah IS, Iswadi IM, Khairul O, Nurhazilah M, Fadzilah MS, Padzil AR, et al.
    Clin Ter, 2010;161(2):125-8.
    PMID: 20499025
    There is an association between reactive oxygen species (ROS) and DNA damage to sperm. Researchers believe that ROS is always present at the sperm's head. The variation of ROS concentration within the area has an impact on the integrity of the DNA.
    MeSH terms: Animals; Goats; Male; Spermatozoa/metabolism*; Reactive Oxygen Species*; Microscopy, Confocal
  9. Noranizah W, Siti-Aishah MA, Munirah MA, Norazlin MH, Rohaizak M, Naqiyah I, et al.
    Clin Ter, 2010;161(2):129-37.
    PMID: 20499026
    Vascular endothelial growth factor (VEGF) is a leading factor for tumour angiogenesis and p53 protein is the product of a tumor suppressor gene. The main aim of the study was to assess the association of p53 protein with VEGF expression in breast carcinoma.
    MeSH terms: Adolescent; Adult; Aged; Breast/metabolism*; Breast/pathology*; Breast/chemistry; Breast Neoplasms/metabolism*; Breast Neoplasms/chemistry; Female; Humans; Hyperplasia; Immunohistochemistry; Middle Aged; Retrospective Studies; Tumor Suppressor Protein p53/analysis; Tumor Suppressor Protein p53/biosynthesis*; Vascular Endothelial Growth Factor A/analysis*; Vascular Endothelial Growth Factor A/biosynthesis*; Young Adult
  10. Nur Haslindawaty AR, Panneerchelvam S, Edinur HA, Norazmi MN, Zafarina Z
    Int J Legal Med, 2010 Sep;124(5):415-26.
    PMID: 20502908 DOI: 10.1007/s00414-010-0469-x
    The uniparentally inherited mitochondrial DNA (mtDNA) is in the limelight for the past two decades, in studies relating to demographic history of mankind and in forensic kinship testing. In this study, human mtDNA hypervariable segments 1, 2, and 3 (HV1, HV2, and HV3) were analyzed in 248 unrelated Malay individuals in Peninsular Malaysia. Combined analyses of HV1, HV2, and HV3 revealed a total of 180 mtDNA haplotypes with 149 unique haplotypes and 31 haplotypes occurring in more than one individual. The genetic diversity was estimated to be 99.47%, and the probability of any two individuals sharing the same mtDNA haplotype was 0.93%. The most frequent mtDNA haplotype (73, 146, 150, 195, 263, 315.1C, 16140, 16182C, 16183C, 16189, 16217, 16274, and 16335) was shared by 11 (4.44%) individuals. The nucleotide diversity and mean of pair-wise differences were found to be 0.036063 ± 0.020101 and 12.544022 ± 6.230486, respectively.
    MeSH terms: DNA, Mitochondrial/genetics*; Genetics, Population; Haplotypes; Humans; Malaysia; Polymorphism, Genetic*; Polymerase Chain Reaction; DNA Fingerprinting; Sequence Analysis, DNA; Complementarity Determining Regions/genetics*
  11. Abdo A, Chen B, Mueller C, Salim N, Willett P
    J Chem Inf Model, 2010 Jun 28;50(6):1012-20.
    PMID: 20504032 DOI: 10.1021/ci100090p
    A Bayesian inference network (BIN) provides an interesting alternative to existing tools for similarity-based virtual screening. The BIN is particularly effective when the active molecules being sought have a high degree of structural homogeneity but has been found to perform less well with structurally heterogeneous sets of actives. In this paper, we introduce an alternative network model, called a Bayesian belief network (BBN), that seeks to overcome this limitation of the BIN approach. Simulated virtual screening experiments with the MDDR, WOMBAT and MUV data sets show that the BIN and BBN methods allow effective screening searches to be carried out. However, the results obtained are not obviously superior to those obtained using a much simpler approach that is based on the use of the Tanimoto coefficient and of the square roots of fragment occurrence frequencies.
    MeSH terms: Bayes Theorem; Drug Evaluation, Preclinical/methods*; Ligands; Proteins/metabolism; User-Computer Interface*
  12. Latiff ZA, Kamal NA, Jahendran J, Alias H, Goh BS, Syed Zakaria SZ, et al.
    J Pediatr Hematol Oncol, 2010 Jul;32(5):407-10.
    PMID: 20505534 DOI: 10.1097/MPH.0b013e3181e01584
    Vincristine-induced vocal cord paralysis is a rare but serious complication. We report 2 patients with acute lymphoblastic leukemia who developed progressive stridor during induction chemotherapy. There were no clinical features of peripheral or autonomic neuropathy. Flexible laryngoscopy confirmed the diagnosis of bilateral vocal cord palsy; interestingly, the nerve conduction test revealed axonal motor neuropathy involving the median and common peroneal nerves in both patients. The first patient required prolonged ventilatory support necessitating unilateral cordectomy before extubation, whereas the second only required supplemental oxygen therapy. There was resolution of stridor in the first patient after cordectomy and gradual clinical improvement in the second. These cases illustrate that a high index of suspicion of vincristine-induced vocal cord palsy with prompt otolaryngology consultation for laryngoscopy is required in the diagnostic evaluation of a patient who has received vincristine.
    MeSH terms: Antineoplastic Agents, Phytogenic/adverse effects*; Child, Preschool; Female; Humans; Laryngoscopy; Male; Vincristine/adverse effects*; Vocal Cord Paralysis/chemically induced*; Vocal Cord Paralysis/drug therapy; Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy*
  13. Hasan SS, Alen YK, Wayne WG, Ahmadi K, Anwar M, Goh GK
    Singapore Med J, 2010 Apr;51(4):290-9.
    PMID: 20505906
    People with epilepsy are socially discriminated against on the grounds of widespread negative public attitudes, misunderstandings and defensive behaviour. The primary purpose of this study was to evaluate the public understanding of and attitudes toward epilepsy among the Chinese population in Malaysia.
    MeSH terms: Adolescent; Adult; Aged; Attitude to Health; China; Educational Status; Epilepsy/ethnology*; Epilepsy/epidemiology*; Female; Humans; Health Knowledge, Attitudes, Practice; Malaysia; Male; Middle Aged; Surveys and Questionnaires; Urban Population
  14. Ng CH, Lee KT, Taib NA, Yip CH
    Singapore Med J, 2010 Apr;51(4):306-10.
    PMID: 20505908
    With an increasing number of women undergoing breast screening, an effective method of removing non-palpable lumps detected by mammography or sonography is by hookwire localisation excision biopsy (HWLB). The aim of this paper was to audit the practice of HWLB at the University Malaya Medical Centre.
    MeSH terms: Adult; Aged; Biopsy/methods*; Biopsy, Needle/instrumentation; Biopsy, Needle/methods*; Breast Neoplasms/diagnosis*; Breast Neoplasms/surgery*; Female; Humans; Malaysia; Middle Aged; Sensitivity and Specificity; Treatment Outcome; Biopsy, Fine-Needle/instrumentation; Biopsy, Fine-Needle/methods*
  15. Nalliah S, Chan SL, Ong CL, Suthan TH, Tan KC, She VN, et al.
    Singapore Med J, 2010 Apr;51(4):332-8.
    PMID: 20505913
    Internet search has been the main source for information and data mining in medical research. Its use by medical students has immensely contributed to learning activities. The main aim of the study was to determine the effectiveness of internet use by medical students during their initial years of clinical instruction in order to establish a diagnosis after being provided with the history and physical findings of a clinical problem.
    MeSH terms: Decision Support Techniques*; Diagnosis; Education, Medical; Evaluation Studies as Topic; Humans; Students, Medical*; Reproducibility of Results; Information Storage and Retrieval; Internet; Search Engine*
  16. Ku CS, Pawitan Y, Sim X, Ong RT, Seielstad M, Lee EJ, et al.
    Hum Mutat, 2010 Jul;31(7):851-7.
    PMID: 20506136 DOI: 10.1002/humu.21287
    Research on the role of copy number variations (CNVs) in the genetic risk of diseases in Asian populations has been hampered by a relative lack of reference CNV maps for Asian populations outside the East Asians. In this article, we report the population characteristics of CNVs in Chinese, Malay, and Asian Indian populations in Singapore. Using the Illumina Human 1M Beadchip array, we identify 1,174 CNV loci in these populations that corroborated with findings when the same samples were typed on the Affymetrix 6.0 platform. We identify 441 novel loci not previously reported in the Database of Genomic Variations (DGV). We observe a considerable number of loci that span all three populations and were previously unreported, as well as population-specific loci that are quite common in the respective populations. From this we observe the distribution of CNVs in the Asian Indian population to be considerably different from the Chinese and Malay populations. About half of the deletion loci and three-quarters of duplication loci overlap UCSC genes. Tens of loci show population differentiation and overlap with genes previously known to be associated with genetic risk of diseases. One of these loci is the CYP2A6 deletion, previously linked to reduced susceptibility to lung cancer.
    MeSH terms: Aryl Hydrocarbon Hydroxylases/genetics; Chromosome Mapping; Genetics, Population; Humans; India/ethnology; Lung Neoplasms/genetics; Malaysia/ethnology; Singapore; Genetic Variation*; Gene Deletion; Gene Dosage/genetics*; Genetic Predisposition to Disease/ethnology; Genetic Predisposition to Disease/genetics*; Gene Duplication; Principal Component Analysis; Asian Continental Ancestry Group/ethnology; Asian Continental Ancestry Group/genetics*; Cytochrome P-450 CYP2A6
  17. Najiah M, Nadirah M, Sakri I, Shaharom-Harrison F
    Pak J Biol Sci, 2010 Mar 15;13(6):293-7.
    PMID: 20506717
    A study was carried out to investigate the presence of bacteria flora in wild mud crab (Scylla serrata) from Setiu Wetland as well as their antibiotic resistances. A total of 91 bacterial isolates consisting of 12 bacterial species were successfully isolated from mud crab. Oxolinic acid was found to be effective against all the bacterial isolates whilst the highest percentage of antibiotic resistance was shown by lincomycin (94.5%) followed by ampicillin (90.1%), amoxicillin (86.8%) and oleandomycin (78.0%). The study is very useful to evaluate the safety of mud crab for human consumption based on wild mud crab-associated bacteria as well as their antibiotic resistances.
    MeSH terms: Animals; Bacteria/classification; Bacteria/isolation & purification*; Crustacea/microbiology*; Drug Resistance, Microbial*; Malaysia; Microbial Sensitivity Tests
  18. Omar MN, Nor-Nazuha MN, Nor-Dalilah MN, Sahri MM
    Pak J Biol Sci, 2010 Mar 15;13(6):298-302.
    PMID: 20506718
    In order to evaluate the frying performance of palm-based solid frying shortening against standard olein, the fresh potato chips were fried in both frying media using an open fryer. After frying the chips for 40 h in an open batch fryer, it was found that the frying quality of palm-based solid frying shortening was better than standard palm olein in terms of Free Fatty Acid (FFA) values, Total Polar Content (TPC) and Total Polymeric Material (TPM). Solid shortening gave FFA, TPC and TPM values of 0.7, 15.3 and 2.67%, respectively, whilst standard palm olein gave values for FFA, TPC and TPM of 1.2, 19.6 and 3.10%, respectively. In terms of sensory mean scores, sensory panelists preferred the color of potato chips fried in solid shortening on the first day of frying, while on the third and fifth day of frying there were no significant differences (p < 0.05) in the sensory scores of fried products in both frying mediums. However, on the fifth day of frying, panelists gave higher scores in terms of taste, flavor and crispness for potato chips fried in solid shortening. These findings show that the palm-based solid shortening is better than palm olein when used for deep fat frying in terms of FFA values, total polar content and total polymeric material, especially for starch-based products such as potato chips. The result also shows that, in terms of sensory mean scores, after frying for 40 h, the sensory panelists gave higher scores in terms of taste, flavor and crispiness for potato chips fried in palm-based solid shortening.
    MeSH terms: Chromatography, High Pressure Liquid; Cooking*; Humans; Plant Oils*
  19. Govindaraju R, Rajagopalan R, Omar R, Mukari SA
    Congenit Anom (Kyoto), 2010 Sep;50(3):193-6.
    PMID: 20507348 DOI: 10.1111/j.1741-4520.2010.00280.x
    We report a rare case of laryngotracheal anomaly and its possible etiology and mode of presentation. A teenager presented with voice change and a neck lump. Investigations revealed a laryngeal anomaly in which the larynx was hyperdescended. It was accompanied by low lying thyroid gland and hyoid bone together with an absence of a cervical segment of the esophagus and trachea. The anomaly only became noticeable secondary to pubertal changes in the thyroid cartilage of the teenager. An embryological defect during the formation of the laryngotracheal tube and esophagus is a possible explanation of this anomaly. The present case probably represents the third reported of its kind.
    MeSH terms: Adolescent; Humans; Hyoid Bone/abnormalities; Hyoid Bone/radiography; Larynx/abnormalities*; Larynx/radiography; Male; Puberty; Thyroid Cartilage/abnormalities; Thyroid Cartilage/radiography; Tomography, X-Ray Computed; Trachea/abnormalities
  20. Shaariyah MM, Mazita A, Masaany M, Razif MY, Isa MR, Asma A
    Chin J Cancer, 2010 Jun;29(6):631-3.
    PMID: 20507738
    Synovial sarcoma is a rare soft tissue sarcoma of the head and neck region involving the parapharyngeal space. The diagnosis of synovial sarcoma can be very challenging to the pathologists. We present a rare case of parapharyngeal synovial sarcoma in a young female patient who had a two-month history of left cervical intumescent mass at level II. The fine needle aspiration cytology of the mass was proved inconclusive. Transcervical excision of the mass was performed and the first case of parapharyngeal sarcoma was identified in our center by fluorescence in situ hybridization (FISH) technique. Repeat imaging revealed residual tumor. The patient successfully underwent a second excision of the residual tumor and received adjuvant radiotherapy.
    MeSH terms: Adult; Combined Modality Therapy; Female; Humans; Pharyngeal Neoplasms/diagnosis*; Pharyngeal Neoplasms/pathology; Pharyngeal Neoplasms/radiotherapy; Pharyngeal Neoplasms/surgery*; Sarcoma, Synovial/diagnosis*; Sarcoma, Synovial/pathology; Sarcoma, Synovial/radiotherapy; Sarcoma, Synovial/surgery*; Tomography, X-Ray Computed; In Situ Hybridization, Fluorescence; Neoplasm, Residual; Radiotherapy, Adjuvant; Biopsy, Fine-Needle
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