Browse publications by year: 2010

  1. Hydrophobic protein in colorectal cancer in relation to tumor stages and grades
    Yeoh LC, Loh CK, Gooi BH, Singh M, Gam LH
    World J Gastroenterol, 2010 Jun 14;16(22):2754-63.
    PMID: 20533595
    AIM: To identify differentially expressed hydrophobic proteins in colorectal cancer.

    METHODS: Eighteen pairs of colorectal cancerous tissues in addition to tissues from normal mucosa were analysed. Hydrophobic proteins were extracted from the tissues, separated using 2-D gel electrophoresis and analysed using Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS). Statistical analysis of the proteins was carried out in order to determine the significance of each protein to colorectal cancer (CRC) and also their relation to CRC stages, grades and patients' gender.

    RESULTS: Thirteen differentially expressed proteins which were expressed abundantly in either cancerous or normal tissues were identified. A number of these proteins were found to relate strongly with a particular stage or grade of CRC. In addition, the association of these proteins with patient gender also appeared to be significant.

    CONCLUSION: Stomatin-like protein 2 was found to be a promising biomarker for CRC, especially in female patients. The differentially expressed proteins identified were associated with CRC and may act as drug target candidates.

    MeSH terms: Adult; Aged; Aged, 80 and over; Blood Proteins/chemistry; Chromatography, Liquid; Female; Humans; Male; Membrane Proteins/chemistry; Middle Aged; Molecular Sequence Data; Neoplasm Proteins/chemistry*; Neoplasm Staging; Biomarkers, Tumor*; Colorectal Neoplasms/pathology*; Colorectal Neoplasms/chemistry*; Electrophoresis, Gel, Two-Dimensional; Protein Array Analysis; Tandem Mass Spectrometry; Hydrophobic and Hydrophilic Interactions
  2. Fulltext The differential expression of aqueous soluble proteins in breast normal and cancerous tissues in relation to ethnicity of the patients; Chinese, Malay and Indian
    Liang S, Singh M, Gam LH
    Dis Markers, 2010;28(3):149-65.
    PMID: 20534901 DOI: 10.3233/DMA-2010-0694
    Female breast cancer is one of the leading causes of female mortality worldwide. In Malaysia, breast cancer is the most commonly diagnosed cancer in women. Of the women in Malaysia, the Chinese have the highest number of breast cancer cases, followed by the Indian and the Malay. The most common type of breast cancer is infiltrating ductal carcinoma (IDC). A proteomic approach was applied in this study to identify changes in the protein profile of cancerous tissues compared with normal tissues from 18 patients; 8 Chinese, 6 Malay and 4 Indian were analysed. Twenty-four differentially expressed hydrophilic proteins were identified. We evaluated the potential of these proteins as biomarkers for infiltrating ductal carcinoma based on their ethnic-specific expressions. Three of the upregulated proteins, calreticulin, 14-3-3 protein zeta and 14-3-3 protein eta, were found to be expressed at a significantly higher level in the cancerous breast tissues when compared with the normal tissues in cases of infiltrating ductal carcinoma. The upregulation in expression was particularly dominant in the Malay cohort.
    MeSH terms: Aged; Aged, 80 and over; Breast/metabolism*; China; Chromatography, Liquid; Ethnic Groups*; Female; Humans; India; Malaysia; Middle Aged; Neoplasm Proteins/metabolism*; Proteins/metabolism*; Solubility; Water; Electrophoresis, Gel, Two-Dimensional; Cohort Studies; Tandem Mass Spectrometry
  3. Fulltext Hemoglobin variants detected by hemoglobin A1c (HbA1c) analysis and the effects on HbA1c measurements
    Nasir NM, Thevarajah M, Yean CY
    Int J Diabetes Dev Ctries, 2010 Apr;30(2):86-90.
    PMID: 20535312 DOI: 10.4103/0973-3930.62598
    Hemoglobin (Hb) A1c is a tool widely used to monitor long-term glycemic control in diabetic patients. The objective of our study is to compare the HbA1c values measured on high performance liquid chromatography (HPLC) and immunoassay in patients who were detected to have hemoglobin variant after HbA1c analysis.
  4. Fulltext Educational program for patients with type-1 diabetes mellitus receiving free monthly supplies of insulin improves knowledge and attitude, but not adherence
    Salam A
    Int J Diabetes Dev Ctries, 2010 Apr;30(2):105.
    PMID: 20535317 DOI: 10.4103/0973-3930.62602
    Comment on: Vimalavathini R, Agarwal SM, Gitanjali B. Educational program for patients with type-1 diabetes mellitus receiving free monthly supplies of insulin improves knowledge and attitude, but not adherence. Int J Diab Dev Ctries. 2008;28:86–90
    MeSH terms: Diabetes Mellitus, Type 1; Health Education; Humans
  5. Fulltext Is there a genetic variation association in the IL-10 and TNF alpha promoter gene with gestational diabetes mellitus?
    Montazeri S, Nalliah S, Radhakrishnan AK
    Hereditas, 2010 Apr;147(2):94-102.
    PMID: 20536548 DOI: 10.1111/j.1601-5223.2009.02134.x
    Gestational diabetes mellitus (GDM), defined as carbohydrate intolerance diagnosed for the first time during pregnancy, affects both maternal and fetal health. Possession of a specific genetic polymorphism can be a predisposing factor for susceptibility to some diseases. The aim of this study was to investigate the association between single nucleotide polymorphisms (SNP) in the promoter gene of interleukin-10 (IL-10) as well as tumor necrosis factor-alpha (TNF alpha) with the development of GDM. Two hundred and twelve consecutive series of eligible normal pregnant women (controls) and gestational diabetes mellitus women were selected based on the study's inclusion and exclusion criteria. DNA was extracted from blood and genotyped for IL-10 at three positions and TNF alpha for gene polymorphism using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Plasma levels of IL-10 and TNF alpha at different gestational periods as well as postpartum were quantified using enzyme linked immunosorbent assay (ELISA). The results of the study showed that the difference in the frequency of SNP at position -597 in the promoter of the human IL-10 gene between the control and GDM groups was statistically significant (p < 0.05). In contrast, there was no significant difference in the frequency of SNP at the other two sites in the promoter region of the human IL-10 gene (-824 and -1082) as well as position -308 in the promoter of the human TNF-alpha (p > 0.05). In addition, there was no significant difference between the two groups in terms of plasma levels of IL-10 as well as TNF alpha in different stages of pregnancy. SNP at position -597 was significantly associated with the development of GDM and shows potential for use as a predictive marker for GDM.
    MeSH terms: Adult; Female; Humans; Middle Aged; Pregnancy; Promoter Regions, Genetic*; Tumor Necrosis Factor-alpha/genetics*; Genetic Variation*; Case-Control Studies; Diabetes, Gestational/genetics*; Interleukin-10/genetics*
  6. Determinants of subjective quality of life in first-episode schizophrenia: perspective from Malaysia
    Chee KY
    Early intervention in psychiatry, 2010 May;4(2):111-8.
    PMID: 20536966 DOI: 10.1111/j.1751-7893.2010.00176.x
    This study sought to examine the determinants of subjective quality of life among patients with first-episode schizophrenia in a developing country.
    MeSH terms: Adult; Cross-Sectional Studies; Developing Countries; Female; Humans; Malaysia; Male; Quality of Life/psychology*; Schizophrenia/diagnosis*; Schizophrenic Psychology*; Sex Characteristics
  7. Fulltext Candida glabrata endophthalmitis following penetrating keratoplasty in a patient with negative donor rim culture
    Muzaliha MN, Adil H, Ibrahim M, Shatriah I
    BMC Ophthalmol, 2010;10:18.
    PMID: 20537193 DOI: 10.1186/1471-2415-10-18
    Candida glabrata endophthalmitis following keratoplasty is rare and almost always associated with positive donor rim culture.
    MeSH terms: Abscess/etiology; Abscess/pathology; Abscess/ultrasonography; Administration, Topical; Amphotericin B/administration & dosage; Antifungal Agents/administration & dosage; Candidiasis/etiology*; Drug Administration Schedule; Eye Diseases/etiology; Eye Diseases/ultrasonography; Fuchs' Endothelial Dystrophy/surgery*; Humans; Injections, Intravenous; Male; Middle Aged; Endophthalmitis/microbiology*; Endophthalmitis/pathology; Sutures/adverse effects; Vitreous Body/ultrasonography; Keratoplasty, Penetrating/adverse effects*; Transplants/microbiology*; Candida glabrata/isolation & purification*; Injections, Intraocular
  8. Adsorption of methyl orange from aqueous solution onto calcined Lapindo volcanic mud
    Jalil AA, Triwahyono S, Adam SH, Rahim ND, Aziz MA, Hairom NH, et al.
    J Hazard Mater, 2010 Sep 15;181(1-3):755-62.
    PMID: 20538408 DOI: 10.1016/j.jhazmat.2010.05.078
    In this study, calcined Lapindo volcanic mud (LVM) was used as an adsorbent to remove an anionic dye, methyl orange (MO), from an aqueous solution by the batch adsorption technique. Various conditions were evaluated, including initial dye concentration, adsorbent dosage, contact time, solution pH, and temperature. The adsorption kinetics and equilibrium isotherms of the LVM were studied using pseudo-first-order and -second-order kinetic equations, as well as the Freundlich and Langmuir models. The experimental data obtained with LVM fits best to the Langmuir isotherm model and exhibited a maximum adsorption capacity (q(max)) of 333.3 mg g(-1); the data followed the second-order equation. The intraparticle diffusion studies revealed that the adsorption rates were not controlled only by the diffusion step. The thermodynamic parameters, such as the changes in enthalpy, entropy, and Gibbs free energy, showed that the adsorption is endothermic, random and spontaneous at high temperature. The results indicate that LVM adsorbs MO efficiently and could be utilized as a low-cost alternative adsorbent for the removal of anionic dyes in wastewater treatment.
    MeSH terms: Adsorption; Azo Compounds/isolation & purification*; Hydrogen-Ion Concentration; Indicators and Reagents; Kinetics; Solutions; Temperature; Thermodynamics; Water Pollutants, Chemical/isolation & purification*; Waste Management/methods; Water Purification/methods*; Volcanic Eruptions
  9. Purification of bacteriophage M13 by anion exchange chromatography
    Monjezi R, Tey BT, Sieo CC, Tan WS
    J Chromatogr B Analyt Technol Biomed Life Sci, 2010 Jul 1;878(21):1855-9.
    PMID: 20538529 DOI: 10.1016/j.jchromb.2010.05.028
    M13 is a non-lytic filamentous bacteriophage (phage). It has been used widely in phage display technology for displaying foreign peptides, and also for studying macromolecule structures and interactions. Traditionally, this phage has been purified by cesium chloride (CsCl) density gradient ultracentrifugation which is highly laborious and time consuming. In the present study, a simple, rapid and efficient method for the purification of M13 based on anion exchange chromatography was established. A pre-packed SepFast Super Q column connected to a fast protein liquid chromatography (FPLC) system was employed to capture released phages in clarified Escherichia coli fermented broth. An average yield of 74% was obtained from a packed bed mode elution using citrate buffer (pH 4), containing 1.5 M NaCl at 1 ml/min flow rate. The purification process was shortened substantially to less than 2 h from 18 h in the conventional ultracentrifugation method. SDS-PAGE revealed that the purity of particles was comparable to that of CsCl gradient density ultracentrifugation method. Plaque forming assay showed that the purified phages were still infectious.
    MeSH terms: Anions/chemistry; Centrifugation, Density Gradient; Cesium; Chlorides; Chromatography, Ion Exchange/methods*; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Osmolar Concentration; Viral Plaque Assay; Bacteriophage M13/isolation & purification*; Bacteriophage M13/chemistry
  10. Unhealthy travelers present challenges to sustainable primate ecotourism
    Muehlenbein MP, Martinez LA, Lemke AA, Ambu L, Nathan S, Alsisto S, et al.
    Travel Med Infect Dis, 2010 May;8(3):169-75.
    PMID: 20541137 DOI: 10.1016/j.tmaid.2010.03.004
    Ecotourism can function as a powerful tool for species conservation. However, a significant proportion of travelers at wildlife sanctuaries may be ill and potentially infectious, creating unnecessary risk of pathogen transmission to wildlife.
    MeSH terms: Adolescent; Adult; Aged; Animals; Animals, Wild*; Female; Humans; Male; Middle Aged; Surveys and Questionnaires; Travel*; Zoonoses/transmission*; Logistic Models; Disease Transmission, Infectious/veterinary*; Risk Assessment; Pongo*
  11. Wavelet energy-guided level set-based active contour: a segmentation method to segment highly similar regions
    Achuthan A, Rajeswari M, Ramachandram D, Aziz ME, Shuaib IL
    Comput Biol Med, 2010 Jul;40(7):608-20.
    PMID: 20541182 DOI: 10.1016/j.compbiomed.2010.04.005
    This paper introduces an approach to perform segmentation of regions in computed tomography (CT) images that exhibit intra-region intensity variations and at the same time have similar intensity distributions with surrounding/adjacent regions. In this work, we adapt a feature computed from wavelet transform called wavelet energy to represent the region information. The wavelet energy is embedded into a level set model to formulate the segmentation model called wavelet energy-guided level set-based active contour (WELSAC). The WELSAC model is evaluated using several synthetic and CT images focusing on tumour cases, which contain regions demonstrating the characteristics of intra-region intensity variations and having high similarity in intensity distributions with the adjacent regions. The obtained results show that the proposed WELSAC model is able to segment regions of interest in close correspondence with the manual delineation provided by the medical experts and to provide a solution for tumour detection.
    MeSH terms: Algorithms*; Urinary Bladder Neoplasms/pathology; Urinary Bladder Neoplasms/radiography; Humans; Image Interpretation, Computer-Assisted/methods*; Sensitivity and Specificity; Thyroid Neoplasms/pathology; Thyroid Neoplasms/radiography; Tomography, X-Ray Computed/methods*; Normal Distribution
  12. Sludge palm oil as a renewable raw material for biodiesel production by two-step processes
    Hayyan A, Alam MZ, Mirghani ME, Kabbashi NA, Hakimi NI, Siran YM, et al.
    Bioresour Technol, 2010 Oct;101(20):7804-11.
    PMID: 20541401 DOI: 10.1016/j.biortech.2010.05.045
    In this study, biodiesel was produced from sludge palm oil (SPO) using tolune-4-sulfonic monohydrate acid (PTSA) as an acid catalyst in different dosages in the presence of methanol to convert free fatty acid (FFA) to fatty acid methyl ester (FAME), followed by a transesterification process using an alkaline catalyst. In the first step, acid catalyzed esterification reduced the high FFA content of SPO to less than 2% with the different dosages of PTSA. The optimum conditions for pretreatment process by esterification were 0.75% (w/w) dosage of PTSA to SPO, 10:1 M ratio, 60 °C temperature, 60 min reaction time and 400 rpm stirrer speed. The highest yield of biodiesel after transesterification and purification processes was 76.62% with 0.07% FFA and 96% ester content. The biodiesel produced was favorable as compared to EN 14214 and ASTM 6751 standard. This study shows a potential exploitation of SPO as a new feedstock for the production of biodiesel.
    MeSH terms: Catalysis; Esterification; Fatty Acids/metabolism; Kinetics; Gas Chromatography-Mass Spectrometry; Plant Oils*; Sewage*; Biofuels*
  13. Hypoglycemic symptoms in patients with type 2 diabetes in Asia-Pacific-Real-life effectiveness and care patterns of diabetes management: the RECAP-DM study
    Chan SP, Ji LN, Nitiyanant W, Baik SH, Sheu WH
    Diabetes Res Clin Pract, 2010 Aug;89(2):e30-2.
    PMID: 20541826 DOI: 10.1016/j.diabres.2010.05.008
    Symptoms of hypoglycemia were reported by 35.8% of patients with type 2 diabetes treated with oral antihyperglycemic agents in the Asia-Pacific region. Symptoms were severe in 11.6% and very severe in 8.2% of patients experiencing hypoglycemia.
    MeSH terms: Aged; Cross-Sectional Studies; Diabetes Mellitus, Type 2/blood*; Diabetes Mellitus, Type 2/drug therapy*; Diabetes Mellitus, Type 2/metabolism; Female; Humans; Hypoglycemia/blood*; Hypoglycemia/metabolism; Hypoglycemia/physiopathology; Hypoglycemic Agents/therapeutic use; Malaysia; Male; Metformin/therapeutic use; Middle Aged; Sulfonylurea Compounds/therapeutic use
  14. Biosynthesis and mobilization of a novel polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer produced by Burkholderia sp. USM (JCM15050)
    Lau NS, Chee JY, Tsuge T, Sudesh K
    Bioresour Technol, 2010 Oct;101(20):7916-23.
    PMID: 20541932 DOI: 10.1016/j.biortech.2010.05.049
    We attempted to synthesize a polyhydroxyalkanoate (PHA) containing newly reported 3-hydroxy-4-methylvalerate (3H4MV) monomer by using wild type Burkholderia sp. USM (JCM15050) and its transformed strain harboring the PHA synthase gene of Aeromonas caviae (phaCAc). The introduction of 3H4MV as a second monomer will improve the material properties of 3HB-based polymers. To promote the accumulation of PHA containing 3H4MV monomer, isocaproic acid was provided as co-carbon source. Approximately 1mol% of 3H4MV was detected in wild type Burkholderia sp. cultures when they were fed glucose or fructose together with isocaproic acid. Thus, the wild type strain can synthesize the 3H4MV monomer. High 3H4MV fractions, of about 40mol%, were obtained when the transformed strain was cultivated on glucose or fructose together with isocaproic acid. In addition, the ability of the transformed strain to mobilize accumulated PHA containing 3H4MV monomer was demonstrated in this study. This is the first report on mobilization of the 3H4MV monomer.
    MeSH terms: Magnetic Resonance Spectroscopy; Pentanoic Acids/metabolism*; Burkholderia/metabolism*; Burkholderia/ultrastructure; Microscopy, Electron, Transmission; Polyhydroxyalkanoates/metabolism*
  15. Aspidospermatan-aspidospermatan and eburnane-sarpagine bisindole alkaloids from Leuconotis
    Gan CY, Low YY, Robinson WT, Komiyama K, Kam TS
    Phytochemistry, 2010 Aug;71(11-12):1365-70.
    PMID: 20542302 DOI: 10.1016/j.phytochem.2010.05.015
    Leucofoline and leuconoline, representing the first members of the aspidospermatan-aspidospermatan and eburnane-sarpagine subclasses of the bisindole alkaloids, respectively, were isolated from the Malayan Leuconotis griffithii. The structures of these bisindole alkaloids were established using NMR and MS analysis, and in the case of leuconoline, confirmed by X-ray diffraction analysis. Both alkaloids showed weak cytotoxicity towards human KB cells.
    MeSH terms: Antineoplastic Agents, Phytogenic/isolation & purification*; Antineoplastic Agents, Phytogenic/pharmacology; Antineoplastic Agents, Phytogenic/chemistry; Drug Screening Assays, Antitumor; Humans; KB Cells; Molecular Conformation; Plants, Medicinal/chemistry*; Stereoisomerism; Molecular Structure; Crystallography, X-Ray; Nuclear Magnetic Resonance, Biomolecular; Indole Alkaloids/isolation & purification*; Indole Alkaloids/pharmacology; Indole Alkaloids/chemistry; Apocynaceae/chemistry*
  16. Idiopathic paroxysmal kinesigenic dyskinesia in Malaysia, a multi-racial Southeast Asian country
    Tai ML, Lim SY, Tan CT
    J Clin Neurosci, 2010 Aug;17(8):1089-90.
    PMID: 20542699 DOI: 10.1016/j.jocn.2009.11.034
    Paroxysmal kinesigenic dyskinesia is a rare disorder, and there are few reports of Asian patients with this condition. We reviewed the clinical features of all patients with idiopathic paroxysmal kinesigenic dyskinesia (PKD) seen at a major neurological centre in Malaysia. The charts of 11 patients with idiopathic PKD seen between 1995 and 2008 were reviewed retrospectively. The male:female ratio was 9:2. Ten patients were of Chinese ethnicity, and one was Malay. Three patients (from two families) had a family history of PKD. The involuntary movement was dystonia in 73% of patients. In one patient, attacks were precipitated by vestibular stimulation. One patient had generalized epilepsy. Another patient who did not have epilepsy demonstrated epileptiform discharges. Only slightly over one-quarter of patients had a positive family history. Males, and people of Chinese ancestry, seem to be affected more frequently by PKD in certain Asian populations.
    MeSH terms: Chorea/diagnosis*; Chorea/physiopathology*; Electroencephalography; Female; Humans; Malaysia; Male; Asian Continental Ancestry Group
  17. Secondhand smoke exposures in indoor public places in seven Asian countries
    Lee J, Lim S, Lee K, Guo X, Kamath R, Yamato H, et al.
    Int J Hyg Environ Health, 2010 Sep;213(5):348-51.
    PMID: 20542729 DOI: 10.1016/j.ijheh.2010.05.007
    Exposure to secondhand smoke (SHS) is a major threat to public health. Asian countries having the highest smoking prevalence are seriously affected by SHS. The objective of the study was to measure SHS levels in hospitality venues in seven Asian countries and to compare the SHS exposure to the levels in Western countries. The study was carried out in four types of related hospitality venues (restaurant, café, bar/club and entertainment) in China, India, Japan, Korea, Malaysia, Pakistan and Sri Lanka. Real-time measurement of particulate matter of <2.5microm aerodynamic diameter (PM(2.5)) was made during business hour using a handheld laser operated monitor. A total of 168 venues were measured in seven countries. The average indoor PM(2.5) level was 137microg/m(3), ranging from 46microg/m(3) in Malaysia to 207microg/m(3) in India. Bar/club had the highest PM(2.5) level of 191microg/m(3) and restaurants had the lowest PM(2.5) level of 92microg/m(3). The average indoor PM(2.5) level in smoking venues was 156micro/m(3), which was 3.6 times higher than non-smoking venues (43microg/m(3)). Indoor PM(2.5) levels were significantly associated with country, type of venue, smoking density and air exchange rate (p<0.05). In the seven Asian countries, PM(2.5) levels were high due to SHS in public places. The current levels are comparable to the levels in Western countries before the adoption of smoke-free policy. Since Asian country has high prevalence of SHS in public places, there is an urgent need for comprehensive smoke-free regulation in Asian countries.
    MeSH terms: Asia; Cross-Sectional Studies; Humans; Restaurants/statistics & numerical data; Tobacco Smoke Pollution/analysis; Tobacco Smoke Pollution/statistics & numerical data*; Air Pollution, Indoor/analysis; Air Pollution, Indoor/statistics & numerical data*; Inhalation Exposure
  18. Fulltext Patient satisfaction and loyalty to the private hospitals in Sana'a, Yemen
    Anbori A, Ghani SN, Yadav H, Daher AM, Su TT
    Int J Qual Health Care, 2010 Aug;22(4):310-5.
    PMID: 20543208 DOI: 10.1093/intqhc/mzq029
    To evaluate patients' satisfaction and loyalty to private hospitals and to identify factors influencing patient loyalty.
    MeSH terms: Adult; Cross-Sectional Studies; Female; Humans; Male; Quality of Health Care/standards; Surveys and Questionnaires; Sex Factors; Socioeconomic Factors; Yemen; Hospitals, Private/standards*; Patient Satisfaction*; Young Adult
  19. Immunization with the recombinant Burkholderia pseudomallei outer membrane protein Omp85 induces protective immunity in mice
    Su YC, Wan KL, Mohamed R, Nathan S
    Vaccine, 2010 Jul 12;28(31):5005-11.
    PMID: 20546831 DOI: 10.1016/j.vaccine.2010.05.022
    Burkholderia pseudomallei is resistant to a wide range of antibiotics, leading to relapse and recrudescence of melioidosis after cessation of antibiotic therapy. More effective immunotherapies are needed for better management of melioidosis. We evaluated the prophylactic potential of the immunogenic outer membrane protein Omp85 as a vaccine against murine melioidosis. Immunization of BALB/c mice with recombinant Omp85 (rOmp85) triggered a Th2-type immune response. Up to 70% of the immunized animals were protected against infectious challenge of B. pseudomallei with reduced bacterial load in extrapulmonary organs. Mouse anti-rOmp85 promoted complement-mediated killing and opsonophagocytosis of B. pseudomallei by human polymorphonuclear cells. In conclusion, we demonstrated that B. pseudomallei Omp85 is potentially able to induce protective immunity against melioidosis.
    MeSH terms: Animals; Antibodies, Bacterial/blood; Antibody Formation; Bacterial Outer Membrane Proteins/immunology*; Bacterial Vaccines/immunology*; Disease Models, Animal; Female; Melioidosis/immunology; Melioidosis/prevention & control*; Mice, Inbred BALB C; Phagocytosis; Recombinant Proteins/immunology; Burkholderia pseudomallei/immunology*; Mice
  20. Anti-inflammatory effect of zerumbone on acute and chronic inflammation models in mice
    Sulaiman MR, Perimal EK, Akhtar MN, Mohamad AS, Khalid MH, Tasrip NA, et al.
    Fitoterapia, 2010 Oct;81(7):855-8.
    PMID: 20546845 DOI: 10.1016/j.fitote.2010.05.009
    The anti-inflammatory activity of zerumbone (1), a natural cyclic sesquiterpene isolated from Zingiber zerumbet Smith was investigated using carrageenan-induced paw edema and cotton pellet-induced granuloma tissue formation test in mice. It was demonstrated that intraperitoneal administration of 1 at a dose of 5, 10, 50 and 100 mg/kg produced significant dose-dependent inhibition of paw edema induced by carrageenan. It was also demonstrated that 1 at similar doses significantly suppressed granulomatous tissue formation in cotton pellet-induced granuloma test.
    MeSH terms: Animals; Anti-Inflammatory Agents/administration & dosage; Anti-Inflammatory Agents/pharmacology; Anti-Inflammatory Agents/therapeutic use*; Carrageenan; Gossypium; Disease Models, Animal; Edema/chemically induced; Edema/drug therapy*; Granuloma/chemically induced; Granuloma/drug therapy*; Inflammation/chemically induced; Inflammation/drug therapy*; Male; Phytotherapy; Mice, Inbred ICR; Plant Extracts/administration & dosage; Plant Extracts/pharmacology; Plant Extracts/therapeutic use*; Sesquiterpenes/administration & dosage; Sesquiterpenes/pharmacology; Sesquiterpenes/therapeutic use*; Rhizome; Zingiberaceae/chemistry*; Mice
External Links