Browse publications by year: 2013

  1. Dilokthornsakul P, Chaiyakunapruk N, Termrungruanglert W, Pratoomsoot C, Saokaew S, Sruamsiri R
    Int. J. Gynecol. Cancer, 2013 Nov;23(9):1544-51.
    PMID: 24172091 DOI: 10.1097/IGC.0b013e3182a80a21
    OBJECTIVE: The potential therapeutic effects of metformin on several cancers were reported. However, the evidence of the effects of metformin on ovarian cancer is still limited and inconclusive. This systematic review and meta-analysis study aim to summarize the existing evidence of the therapeutic effects of metformin on ovarian cancer.

    METHODS: We performed systematic searches using electronic databases including PubMed and EMBASE until December 2012. Key words included "metformin" AND ("ovarian cancer" OR "ovary tumor"). All human studies assessing the effects of metformin on ovarian cancer were eligible for inclusion. All articles were reviewed independently by 2 authors with a standardized approach for the purpose of study, study design, patient characteristics, exposure, and outcomes. The data were pooled using a random-effects model.

    RESULTS: Of 190 studies retrieved, only 3 observational studies and 1 report of 2 randomized controlled trials were included. Among those studies, 2 reported the effects of metformin on survival outcomes of ovarian cancer, whereas the other 2 reported the effects of metformin on ovarian cancer prevention. The findings of studies reporting the effects on survival outcomes indicated that metformin may prolong overall, disease-specific, and progression-free survival in ovarian cancer patients. The results of studies reporting the effects of metformin on ovarian cancer prevention were meta-analyzed. It indicated that metformin tended to decrease occurrence of ovarian cancer among diabetic patients with the pooled odds ratio of 0.57 (95% confidence interval, 0.16-1.99).

    CONCLUSIONS: Our findings showed the potential therapeutic effects of metformin on survival outcomes of ovarian cancer and ovarian cancer prevention. However, most of the evidence was observational studies. There is a call for further well-conducted controlled clinical trials to confirm the effects of metformin on ovarian cancer survival and ovarian cancer prevention.

    MeSH terms: Female; Humans; Hypoglycemic Agents/therapeutic use*; Metformin/therapeutic use*; Ovarian Neoplasms/diagnosis; Ovarian Neoplasms/drug therapy*; Ovarian Neoplasms/mortality; Ovarian Neoplasms/epidemiology; Cohort Studies; Survival Analysis; Case-Control Studies; Treatment Outcome
  2. Armania N, Yazan LS, Ismail IS, Foo JB, Tor YS, Ishak N, et al.
    Molecules, 2013;18(11):13320-39.
    PMID: 24172241 DOI: 10.3390/molecules181113320
    The present research was designed to evaluate the anticancer properties of Dillenia suffruticosa extract. Our focus was on the mode of cell death and cell cycle arrest induced in breast cancer cells by the active fractions (designated as D/F4, D/F5 and EA/P2) derived from chromatographic fractionation of D. suffruticosa extracts. The results showed that the active fractions are more cytotoxic towards MCF-7 (estrogen positive breast cancer cells) and MDA-MB-231 (estrogen negative breast cancer cells) as compared to other selected cancer cell lines that included HeLa, A459 and CaOV3. The induction of cell death through apoptosis by the active fractions on the breast cancer cells was confirmed by Annexin V-FITC and PI staining. Cell cycle analysis revealed that D/F4 and EA/P2 induced G2/M phase cell cycle arrest in MCF-7 cells. On the other hand, MDA-MB-231 cells treated with D/F4 and D/F5 accumulated in the sub-G1 phase without cell cycle arrest, suggesting the induction of cell death through apoptosis. The data suggest that the active fractions of D. suffruticosa extract eliminated breast cancer cells through induction of apoptosis and cell cycle arrest. The reason why MCF-7 was more sensitive towards the treatment than MDA-MB-231 remains unclear. This warrants further work, especially on the role of hormones in response towards cytotoxic agents. In addition, more studies on the mechanisms underlying the induction of apoptosis and cell cycle arrest by the plant extract also need to be carried out.
    MeSH terms: Antineoplastic Agents/therapeutic use; Cell Cycle/drug effects; Female; HeLa Cells; Humans; Plant Extracts/pharmacology*; Plant Extracts/chemistry; Apoptosis/drug effects; Dilleniaceae/chemistry*; Cell Line, Tumor; Cell Proliferation/drug effects; MCF-7 Cells
  3. Abosadiya HM, Hasbullah SA, Mackeen MM, Low SC, Ibrahim N, Koketsu M, et al.
    Molecules, 2013;18(11):13369-84.
    PMID: 24172244 DOI: 10.3390/molecules181113369
    C-5-bromo-2-hydroxyphenylcalix[4]-2-methylresorcinarene (I) was synthesized by cyclocondensation of 5-bromo-2-hydroxybenzaldehyde and 2-methylresorcinol in the presence of concentrated HCl. Compound I was characterized by infrared and nuclear magnetic resonance spectroscopic data. X-ray analysis showed that this compound crystallized in a triclinic system with space group of Pī, a = 15.9592(16)Å, b = 16.9417(17)Å, c = 17.0974(17)Å, α = 68.656(3)°, β = 85.689(3)°, γ = 81.631(3)°, Z = 2 and V = 4258.6(7)Å3. The molecule adopts a chair (C2h) conformation. The thermal properties and antioxidant activity were also investigated. It was strongly antiviral against HSV-1 and weakly antibacterial against Gram-positive bacteria. Cytotoxicity testing on Vero cells showed that it is non-toxic, with a CC50 of more than 0.4 mg/mL.
    MeSH terms: Animals; Anti-Bacterial Agents/pharmacology; Anti-Bacterial Agents/chemistry; Antioxidants/chemistry; Antiviral Agents/pharmacology; Antiviral Agents/chemistry; Cercopithecus aethiops; Phenylalanine/analogs & derivatives*; Phenylalanine/pharmacology; Phenylalanine/chemistry; Thermogravimetry; Vero Cells; Molecular Structure; Herpesvirus 1, Human/drug effects; Crystallography, X-Ray; Calixarenes/pharmacology*; Calixarenes/chemistry*
  4. Reddy SC, Low CK, Lim YP, Low LL, Mardina F, Nursaleha MP
    Nepal J Ophthalmol, 2013 Jul-Dec;5(2):161-8.
    PMID: 24172549 DOI: 10.3126/nepjoph.v5i2.8707
    INTRODUCTION: Computer vision syndrome (CVS) is a condition in which a person experiences one or more of eye symptoms as a result of prolonged working on a computer.
    OBJECTIVES: To determine the prevalence of CVS symptoms, knowledge and practices of computer use in students studying in different universities in Malaysia, and to evaluate the association of various factors in computer use with the occurrence of symptoms.
    MATERIAL AND METHODS: In a cross sectional, questionnaire survey study, data was collected in college students regarding the demography, use of spectacles, duration of daily continuous use of computer, symptoms of CVS, preventive measures taken to reduce the symptoms, use of radiation filter on the computer screen, and lighting in the room.
    RESULTS: A total of 795 students, aged between 18 and 25 years, from five universities in Malaysia were surveyed. The prevalence of symptoms of CVS (one or more) was found to be 89.9%; the most disturbing symptom was headache (19.7%) followed by eye strain (16.4%). Students who used computer for more than 2 hours per day experienced significantly more symptoms of CVS (p=0.0001). Looking at far objects in-between the work was significantly (p=0.0008) associated with less frequency of CVS symptoms. The use of radiation filter on the screen (p=0.6777) did not help in reducing the CVS symptoms.
    CONCLUSION: Ninety percent of university students in Malaysia experienced symptoms related to CVS, which was seen more often in those who used computer for more than 2 hours continuously per day.
    MeSH terms: Adolescent; Adult; Asthenopia/drug therapy; Asthenopia/etiology; Asthenopia/epidemiology*; Computers*; Female; Headache/etiology; Headache/epidemiology*; Humans; Human Engineering/methods; Human Engineering/statistics & numerical data*; Health Knowledge, Attitudes, Practice; Malaysia/epidemiology; Male; Ophthalmic Solutions/administration & dosage; Surveys and Questionnaires; Students/statistics & numerical data*; Syndrome; Universities; Visual Acuity; Prevalence; Young Adult
  5. Kok CM, Sieo CC, Tan HY, Saad WZ, Liang JB, Ho YW
    J Microbiol, 2013 Oct;51(5):700-3.
    PMID: 24173648 DOI: 10.1007/s12275-013-2540-z
    The effect of Leucaena leucocephala hybrid-Bahru (LLB), which contains a high concentration of condensed tannins, on cellulolytic rumen fungal population in goats was investigated using real-time PCR. The fungal population in goats fed LLB was inhibited during the first 10 days of feeding, but after 15 days of feeding, there was a tremendous increase of fungal population (157.0 μg/ml), which was about fourfold more than that in control goats (39.7 μg/ml). However, after this period, the fungal population decreased continuously, and at 30 days of feeding, the fungal population (50.6 μg/ml) was not significantly different from that in control goats (55.4 μg/ml).
    MeSH terms: Anaerobiosis; Animals; Cellulose/metabolism; Diet/methods; Fungi/classification*; Fungi/genetics; Fungi/metabolism*; Goats; Hydrolysis; Fabaceae/metabolism; Rumen/microbiology*; Biota*; Real-Time Polymerase Chain Reaction
  6. Hanapi NA, Ismail S, Mansor SM
    Pharmacognosy Res, 2013 Oct;5(4):241-6.
    PMID: 24174816 DOI: 10.4103/0974-8490.118806
    To date, many findings reveal that most of the modern drugs have the ability to interact with herbal drugs.
    MeSH terms: Phytotherapy; Herb-Drug Interactions
  7. Momtaz YA, Hamid TA, Masud J, Haron SA, Ibrahim R
    Clin Interv Aging, 2013;8:1413-20.
    PMID: 24174873 DOI: 10.2147/CIA.S51877
    BACKGROUND: There is a growing body of literature indicating that attitudes toward aging significantly affect older adults' psychological well-being. However, there is a paucity of scientific investigations examining the role of older adults' attitudes toward aging on their spouses' psychological well-being. Therefore, the present study aimed to examine the dyadic effects of attitude toward aging on the psychological well-being of older couples.
    METHODS: Data for the present study, consisting of 300 couples aged 50 years and older, were drawn from a community-based survey entitled "Poverty among Elderly Women: Case Study of Amanah Ikhtiar" conducted in Peninsular Malaysia. An actor-partner interdependence model using AMOS version 20 (Europress Software, Cheshire, UK) was used to analyze the dyadic data.
    RESULTS: The mean ages of the husbands and wives in this sample were 60.37 years (±6.55) and 56.33 years (±5.32), respectively. Interdependence analyses revealed significant association between older adults' attitudes toward aging and the attitudes of their spouses (intraclass correlation =0.59; P<0.001), and similar interdependence was found for psychological well-being (intraclass correlation =0.57; P<0.001). The findings from AMOS revealed that the proposed model fits the data (CMIN/degrees of freedom =3.23; goodness-of-fit index =0.90; confirmatory fit index =0.91; root mean square error of approximation =0.08). Results of the actor-partner independence model indicated that older adults' psychological well-being is significantly predicted by their spouses' attitudes toward aging, both among older men (critical ratio =2.92; P<0.01) and women (critical ratio =2.70; P<0.01). Husbands' and wives' own reports of their attitudes toward aging were significantly correlated with their own and their spouses' psychological well-being.
    CONCLUSION: The findings from this study supported the proposed Spousal Attitude-Well-Being Model, where older adults' attitudes toward aging significantly affected their own and their spouses' psychological well-being. The theoretical and practical implications of the findings are discussed.
    KEYWORDS: aged; attitude toward aging; psychological well-being
    MeSH terms: Aged; Aging/psychology*; Ambulatory Care Facilities; Attitude to Health*; Cross-Sectional Studies; Female; Health Surveys; Humans; Interpersonal Relations; Malaysia; Male; Mental Health*; Middle Aged; Models, Psychological; Personal Satisfaction*; Spouses/psychology*
  8. Anuar N, Mohd Adnan AF, Saat N, Aziz N, Mat Taha R
    ScientificWorldJournal, 2013;2013:810547.
    PMID: 24174918 DOI: 10.1155/2013/810547
    Anthocyanins not just have various benefits in food industry but also have been used as natural colourants in cosmetic, coating products and as potential natural photosensitizers in solar cell. Thus, the main purpose of this study was to obtain information on the maximum yield of anthocyanin that can be recovered from Melastoma malabathricum fruit. Factors such as extraction temperature, extraction time, and solid to liquid ratio were identified to be significantly affecting anthocyanin extraction efficiency. By using three-level three-factor Box-Behnken design, the optimized conditions for anthocyanin extraction by acidified methanol (R (2) = 0.972) were temperature of 60°C, time of 86.82 min, and 0.5 : 35 (g/mL) solid to liquid ratio while the optimum extraction conditions by acidified ethanol (R (2) = 0.954) were temperature of 60°C, time of 120 min, and 0.5 : 23.06 (g/mL) solid to liquid ratio. The crude anthocyanin extract was further purified by using Amberlite XAD-7 and Sephadex LH-20 column chromatography. Identification of anthocyanins revealed the presence of cyanidin dihexoside, cyanidin hexoside, and delphinidin hexoside as the main anthocyanins in M. malabathricum fruit.
    MeSH terms: Acrylic Resins; Ethanol; Methanol; Anthocyanins/isolation & purification*; Anthocyanins/chemistry; Chromatography, Ion Exchange; Chromatography, Liquid; Dextrans; Chemical Fractionation; Fruit/chemistry*; Models, Chemical; Plant Extracts/chemistry; Polystyrenes; Powders; Solvents; Mass Spectrometry; Temperature; Molecular Structure; Melastomataceae/chemistry*
  9. Oskoueian E, Abdullah N, Oskoueian A
    Biomed Res Int, 2013;2013:349129.
    PMID: 24175289 DOI: 10.1155/2013/349129
    This research was carried out to evaluate the effects of flavone, myricetin, naringin, catechin, rutin, quercetin, and kaempferol at the concentration of 4.5% of the substrate (dry matter basis) on the rumen microbial activity in vitro. Mixture of guinea grass and concentrate (60 : 40) was used as the substrate. The results showed that all the flavonoids except naringin and quercetin significantly (P < 0.05) decreased the dry matter degradability. The gas production significantly (P < 0.05) decreased by flavone, myricetin, and kaempferol, whereas naringin, rutin, and quercetin significantly (P < 0.05) increased the gas production. The flavonoids suppressed methane production significantly (P < 0.05). The total VFA concentration significantly (P < 0.05) decreased in the presence of flavone, myricetin, and kaempferol. All flavonoids except naringin and quercetin significantly (P < 0.05) reduced the carboxymethyl cellulase, filter paperase, xylanase, and β -glucosidase activities, purine content, and the efficiency of microbial protein synthesis. Flavone, myricetin, catechin, rutin, and kaempferol significantly (P < 0.05) reduced the population of rumen microbes. Total populations of protozoa and methanogens were significantly (P < 0.05) suppressed by naringin and quercetin. The results of this research demonstrated that naringin and quercetin at the concentration of 4.5% of the substrate (dry matter basis) were potential metabolites to suppress methane production without any negative effects on rumen microbial fermentation.
    MeSH terms: Animals; Animal Feed; Cattle; Cellulase/metabolism; Fermentation*; Fistula; Flavonoids/administration & dosage*; Male; Methane/metabolism*; Methane/chemistry; Rumen/metabolism; Rumen/microbiology*
  10. Ramli AS, Daher AM, Nor-Ashikin MN, Mat-Nasir N, Ng KK, Miskan M, et al.
    Biomed Res Int, 2013;2013:760963.
    PMID: 24175300 DOI: 10.1155/2013/760963
    Metabolic syndrome (MetS) is a steering force for the cardiovascular diseases epidemic in Asia. This study aimed to compare the prevalence of MetS in Malaysian adults using NCEP-ATP III, IDF, and JIS definitions, identify the demographic factors associated with MetS, and determine the level of agreement between these definitions. The analytic sample consisted of 8,836 adults aged ≥30 years recruited at baseline in 2007-2011 from the Cardiovascular Risk Prevention Study (CRisPS), an ongoing, prospective cohort study involving 18 urban and 22 rural communities in Malaysia. JIS definition gave the highest overall prevalence (43.4%) compared to NCEP-ATP III (26.5%) and IDF (37.4%), P < 0.001. Indians had significantly higher age-adjusted prevalence compared to other ethnic groups across all MetS definitions (30.1% by NCEP-ATP III, 50.8% by IDF, and 56.5% by JIS). The likelihood of having MetS amongst the rural and urban populations was similar across all definitions. A high level of agreement between the IDF and JIS was observed (Kappa index = 0.867), while there was a lower level of agreement between the IDF and NCEP-ATP III (Kappa index = 0.580). JIS definition identified more Malaysian adults with MetS and therefore should be recommended as the preferred diagnostic criterion.
    MeSH terms: Adult; Age Factors; Blood Glucose; Blood Pressure; Cardiovascular Diseases/metabolism; Cardiovascular Diseases/epidemiology; Cardiovascular Diseases/pathology; Cardiovascular Diseases/physiopathology; China/ethnology; Cholesterol; Female; Health Surveys; Humans; India/ethnology; Malaysia; Malaysia/ethnology; Malaysia/epidemiology; Male; Middle Aged; Retrospective Studies; Rural Population; Rural Population*; Triglycerides; Urban Population; Urban Population*; Cohort Studies; Prevalence; Metabolic Syndrome X*; Waist Circumference
  11. Chan GF, Sinniah S, Idris TI, Puad MS, Abd Rahman AZ
    Pak J Biol Sci, 2013 Mar 01;16(5):208-18.
    PMID: 24175430
    Persistent superficial skin infection caused by multiple fungi is rarely reported. Recently, a number of fungi, both opportunistic and persistent in nature were isolated from the foot skin of a 24-year old male in Malaysia. The fungi were identified as Candida parapsilosis, Rhodotorula mucilaginosa, Phoma spp., Debaryomyces hansenii, Acremonium spp., Aureobasidium pullulans and Aspergillus spp., This is the first report on these opportunistic strains were co-isolated from a healthy individual who suffered from persistent foot skin infection which was diagnosed as athlete's foot for more than 12 years. Among the isolated fungi, C. parapsilosis has been an increasingly common cause of skin infections. R. mucilaginosa and D. hansenii were rarely reported in cases of skin infection. A. pullulans, an emerging fungal pathogen was also being isolated in this case. Interestingly, it was noted that C. parapsilosis, R. mucilaginosa, D. hansenii and A. pullulans are among the common halophiles and this suggests the association of halotolerant fungi in causing persistent superficial skin infection. This discovery will shed light on future research to explore on effective treatment for inhibition of pathogenic halophiles as well as to understand the interaction of multiple fungi in the progress of skin infection.
    MeSH terms: Adult; Diagnosis, Differential; Diagnostic Errors; Foot Dermatoses/diagnosis; Foot Dermatoses/microbiology*; Fungi/classification; Fungi/genetics*; Fungi/isolation & purification*; Humans; Malaysia; Male; Opportunistic Infections/diagnosis; Opportunistic Infections/microbiology*; Skin/microbiology*; Tinea Pedis/diagnosis; Tinea Pedis/microbiology*; Young Adult
  12. Sheau-Ting L, Mohammed AH, Weng-Wai C
    J Environ Manage, 2013 Dec 15;131:196-205.
    PMID: 24178312 DOI: 10.1016/j.jenvman.2013.10.001
    This study attempts to identify the optimum social marketing mix for marketing energy conservation behaviour to students in Malaysian universities. A total of 2000 students from 5 major Malaysian universities were invited to provide their preferred social marketing mix. A choice-based conjoint analysis identified a mix of five social marketing attributes to promote energy conservation behaviour; the mix is comprised of the attributes of Product, Price, Place, Promotion, and Post-purchase Maintenance. Each attribute of the mix is associated with a list of strategies. The Product and Post-purchase Maintenance attributes were identified by students as the highest priority attributes in the social marketing mix for energy conservation behaviour marketing, with shares of 27.12% and 27.02%, respectively. The least preferred attribute in the mix is Promotion, with a share of 11.59%. This study proposes an optimal social marketing mix to university management when making decisions about marketing energy conservation behaviour to students, who are the primary energy consumers in the campus. Additionally, this study will assist university management to efficiently allocate scarce resources in fulfilling its social responsibility and to overcome marketing shortcomings by selecting the right marketing mix.
    MeSH terms: Conservation of Natural Resources/methods*; Humans; Social Marketing*
  13. Chelliah KK, Tamanang S, Bt Elias LS, Ying KY
    Indian J Med Sci, 2013 11 2;67(1-2):23-8.
    PMID: 24178338
    BACKGROUND: Two digital mammography systems, based on different physical concepts, have been introduced in the last few years namely the full-field digital mammography (FFDM) system and computed radiography-based mammography using digital storage phosphor plate (DSPM).

    AIMS: The objective of this study was to compare the image quality for DSPM and FFDM using a grading scale based on previously published articles.

    MATERIALS AND METHODS: This comparative diagnostic study was done for 5-month duration at the Breast Clinic. The system used was the Lorad Selenia FFDM system and the Mammomat 3000 Nova DSPM system. The craniocaudal and mediolateral oblique projections were done on both breast on 58 asymptomatic women using both DSPM and FFDM. The mammograms were evaluated for eight criteria of image quality: Tissue coverage, compression, exposure, contrast, resolution, noise, artifact, and sharpness by two independent radiologists.

    STATISTICAL ANALYSIS: Wilcoxon Signed Rank Test and Weighted Kappa.

    RESULTS: FFDM was rated significantly better (P < 0.05) for five aspects: Tissue coverage, compression, contrast, exposure, and resolution and equal to DSPM for sharpness, noise, and artifact.

    CONCLUSION: FFDM was superior in five aspects and equal to DSPM for three aspects of image quality.

    MeSH terms: Adult; Female; Humans; Mammography/instrumentation; Mammography/methods*; Radiographic Image Enhancement/instrumentation; Radiographic Image Enhancement/methods*; X-Ray Intensifying Screens
  14. Mindell JA, Sadeh A, Kwon R, Goh DY
    Sleep, 2013 Nov;36(11):1699-706.
    PMID: 24179304 DOI: 10.5665/sleep.3132
    BACKGROUND:
    To characterize cross-cultural sleep patterns and sleep problems in a large sample of mothers of children (ages birth to 6 years) in multiple predominantly Asian and predominantly Caucasian countries.

    METHODS:
    Mothers of 10,085 young children (predominantly Asian countries/regions: China, Hong Kong, India, Korea, Japan, Malaysia, Philippines, Singapore, Thailand; predominantly Caucasian countries: Australia, Canada, New Zealand, United Kingdom, United States) completed an internet-based expanded version of the Pittsburgh Sleep Quality Index.

    RESULTS:
    Mothers in predominantly Asian countries/regions had later bedtimes, decreased number and duration of night wakings, more nighttime sleep, and more total sleep than mothers from predominantly Caucasian countries, P < 0.001. More than half (54.7%) of mothers reported having poor sleep, ranging from 50.9% of mothers in Malaysia to 77.8% of mothers in Japan. Sleep disturbance symptoms were quite common, especially symptoms related to insomnia, and were more likely to be reported by mothers in predominantly Caucasian countries. However, psychosocial factors, including having children of a younger age, being unemployed, and having a lower education level were the best predictors of poor sleep, whereas culture was not a significant predictor.

    CONCLUSIONS:
    Overall, mothers in predominantly Asian countries/regions reported later bedtimes but sleeping better and longer than mothers from predominantly Caucasian countries, which is dissimilar to cross-cultural findings of young children. Psychosocial factors were found to be the best predictors of poor sleep, irrespective of culture. Further studies are needed to understand the impact of these findings.

    KEYWORDS:
    Sleep; adult; cross-cultural; maternal; mother
    MeSH terms: Adult; Asia, Southeastern/epidemiology; Australia/epidemiology; Canada/epidemiology; Child; Child, Preschool; Cross-Cultural Comparison*; Far East/epidemiology; Female; Great Britain/epidemiology; Humans; India/epidemiology; Infant; Infant, Newborn; Male; Mothers/statistics & numerical data*; New Zealand/epidemiology; Sleep*; United States/epidemiology; Young Adult
  15. Abu Tahir N, Ahmad AH
    J Med Entomol, 2013 Sep;50(5):999-1002.
    PMID: 24180104
    Effects of laterite cover soil with different characteristics on survival of buried eggs, third instar larvae, and pupae of Musca domestica (L.) were studied experimentally. Soil treatments were loose dry soil, loose wet soil, compacted dry soil, and compacted wet soil (CWS). Eggs, third instar larvae, and pupae were buried under 30 cm of the different soil treatments and placed under field conditions until adults emerged. Rearing medium was provided for eggs and larvae, and control treatments of all stages were unburied immatures placed on soil surface. Egg and pupal survival to adult were significantly affected by the cover soil treatments, but third instars were more resilient. Wet soil treatments (loose wet soil and CWS) resulted in significantly reduced pupal survival, but increased survival of eggs. However, CWS significantly reduced adult emergence from buried eggs. Though emergence of house flies buried as eggs was significantly reduced, some were able to hatch and emerging first instar larvae developed to pupation. Although cover soil does not completely prevent fly emergence, it did limit development and emergence of buried house flies.
    MeSH terms: Animals; Houseflies/growth & development; Houseflies/physiology*; Insect Control/methods*; Larva/growth & development; Larva/physiology; Malaysia; Ovum/growth & development; Ovum/physiology; Pupa/growth & development; Pupa/physiology; Soil/chemistry*; Solid Waste*
  16. Bong LJ, Neoh KB, Jaal Z, Lee CY
    J Med Entomol, 2013 Sep;50(5):1003-13.
    PMID: 24180105
    The effects of four temperatures (15, 23.5, 28, and 35 degrees C) on the biological characteristics of the rove beetle Paederus fuscipes Curtis were studied, and its cuticular permeability also was measured. Specimens successfully developed to adulthood at each temperature tested, but development time of each preadult stage significantly decreased with increasing temperature. Both egg and L1 stages required at least 80 degree days above a threshold of approximately 10 degrees C to develop to the subsequent stage. The lengthy development time and high survival rate of preadults at 15 degrees C suggests that P. fuscipes can survive in a harsh environment during cold weather by hibernating, and this ability could allow preadults to succeed ecologically in temperate countries. However, adult longevity was short, and no fecundity was recorded at 15 degrees C. At 28 degrees C, P. fuscipes exhibited a high survival rate of adults, which had a longer life span and high fecundity; thus, the population had the highest intrinsic rate of increase (0.0788 +/- 0.0051 d(-1)) and the shortest mean generation time (48.57 +/- 1.43 d) at 28 degrees C. At this temperature, the population might reach a size that could facilitate invasion into residential areas. However, in the absence of a hygric environment, P. fuscipes was unable to survive despite favorable temperature. Unlike in adults and pupae, high cuticular permeability values were found in the larval stages. This indicates that larvae are highly susceptible to desiccation, and it explains why the distribution of P. fuscipes is restricted to moist habitats.
    MeSH terms: Animals; Beetles/growth & development; Beetles/physiology*; Fertility; Larva/growth & development; Larva/physiology; Longevity; Ovum/growth & development; Ovum/physiology; Permeability; Pupa/growth & development; Pupa/physiology; Temperature; Water/metabolism*; Water Loss, Insensible*
  17. Zuharah WF, Fadzly N, Lester PJ
    J Med Entomol, 2013 Sep;50(5):1014-24.
    PMID: 24180106
    The presence of predators can have dramatic consequences on prey communities, not only by the direct effects of consumption but also through sublethal effects. We investigated the survival rate and subsequent life history of the mosquito Culex pervigilans Bergroth under the influence of its major predator, the backswimmer Anisops wakefieldi White. We established a field experiment with various treatments: 1) control without predators, 2) free-roaming A. wakefieldi (with one, three, or nine A. wakefieldi per container), 3) caged A. wakefieldi (empty cage without predators, with one, three, or nine A. wakefieldi in each cage, and 4) A. wakefieldi cues (with cues concentrations of one, three, or nine A. wakefieldi). Cx. pervigilans eggs were then taken from these four experimental treatments and reared in two different laboratory conditions: 1) in clean water without any traces of predators, or 2) in water with the same treatments as in field. The survival rate of Cx. pervigilans was significantly reduced by the presence of predators or their cues. Even after a brief exposure to waters containing predators or residual cues, the subsequent progeny and the ontogeny of the remaining survivors were still affected. The percentage of eggs that hatched, and the resulting mosquito population, was influenced by the presence of predators or their cues. Our results suggest that sublethal effects may be carried by surviving individuals primarily through the effects of stress, perhaps by epigenetic mechanisms. We may expect to observe similar plasticity in species or populations with high temporal or spatial variability in predation.
    MeSH terms: Animals; Cues*; Culex/growth & development*; Culex/physiology; Larva/growth & development; Larva/physiology; New Zealand; Ovum/growth & development; Ovum/physiology; Pupa/growth & development; Pupa/physiology; Time Factors; Heteroptera/physiology*; Food Chain*
  18. Lau YL, Lee WC, Tan LH, Kamarulzaman A, Syed Omar SF, Fong MY, et al.
    Malar J, 2013 Nov 04;12:389.
    PMID: 24180319 DOI: 10.1186/1475-2875-12-389
    BACKGROUND: Plasmodium ovale is one of the causative agents of human malaria. Plasmodium ovale infection has long been thought to be non-fatal. Due to its lower morbidity, P. ovale receives little attention in malaria research.

    METHODS: Two Malaysians went to Nigeria for two weeks. After returning to Malaysia, they fell sick and were admitted to different hospitals. Plasmodium ovale parasites were identified from blood smears of these patients. The species identification was further confirmed with nested PCR. One of them was successfully treated with no incident of relapse within 12-month medical follow-up. The other patient came down with malaria-induced respiratory complication during the course of treatment. Although parasites were cleared off the circulation, the patient's condition worsened. He succumbed to multiple complications including acute respiratory distress syndrome and acute renal failure.

    RESULTS: Sequencing of the malaria parasite DNA from both cases, followed by multiple sequence alignment and phylogenetic tree construction suggested that the causative agent for both malaria cases was P. ovale curtisi.

    DISCUSSION: In this report, the differences between both cases were discussed, and the potential capability of P. ovale in causing severe complications and death as seen in this case report was highlighted.

    CONCLUSION: Plasmodium ovale is potentially capable of causing severe complications, if not death. Complete travel and clinical history of malaria patient are vital for successful diagnoses and treatment. Monitoring of respiratory and renal function of malaria patients, regardless of the species of malaria parasites involved is crucial during the course of hospital admission.

    MeSH terms: Antimalarials/therapeutic use; Humans; Malaria/complications*; Malaria/diagnosis*; Malaria/drug therapy; Malaria/parasitology; Malaysia; Male; Middle Aged; Molecular Sequence Data; Nigeria; Phylogeny; Respiratory Distress Syndrome, Adult/diagnosis*; Respiratory Distress Syndrome, Adult/etiology*; RNA, Ribosomal, 18S/genetics; Travel; DNA, Protozoan/genetics; DNA, Protozoan/chemistry; Sequence Analysis, DNA; Fatal Outcome; Plasmodium ovale/isolation & purification*; Acute Kidney Injury/diagnosis*; Acute Kidney Injury/etiology*
  19. Chua LS
    J Ethnopharmacol, 2013 Dec 12;150(3):805-17.
    PMID: 24184193 DOI: 10.1016/j.jep.2013.10.036
    Rutin is a common dietary flavonoid that is widely consumed from plant-derived beverages and foods as traditional and folkloric medicine worldwide. Rutin is believed to exhibit significant pharmacological activities, including anti-oxidation, anti-inflammation, anti-diabetic, anti-adipogenic, neuroprotective and hormone therapy. Till date, over 130 registered therapeutic medicinal preparations are containing rutin in their formulations. This article aims to critically review the extraction methods for plant-based rutin and its pharmacological activities. This review provides comprehensive data on the performance of rutin extraction methods and the extent of its pharmacological activities using various in vitro and in vivo experimental models.
    MeSH terms: Animals; Anti-Inflammatory Agents/isolation & purification; Anti-Inflammatory Agents/pharmacology; Anti-Inflammatory Agents/therapeutic use; Antioxidants/isolation & purification; Antioxidants/pharmacology; Antioxidants/therapeutic use; Humans
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