Browse publications by year: 2014

  1. Low VL, Adler PH, Takaoka H, Ya'cob Z, Lim PE, Tan TK, et al.
    PLoS One, 2014;9(6):e100512.
    PMID: 24941043 DOI: 10.1371/journal.pone.0100512
    The population genetic structure of Simulium tani was inferred from mitochondria-encoded sequences of cytochrome c oxidase subunits I (COI) and II (COII) along an elevational gradient in Cameron Highlands, Malaysia. A statistical parsimony network of 71 individuals revealed 71 haplotypes in the COI gene and 43 haplotypes in the COII gene; the concatenated sequences of the COI and COII genes revealed 71 haplotypes. High levels of genetic diversity but low levels of genetic differentiation were observed among populations of S. tani at five elevations. The degree of genetic diversity, however, was not in accordance with an altitudinal gradient, and a Mantel test indicated that elevation did not have a limiting effect on gene flow. No ancestral haplotype of S. tani was found among the populations. Pupae with unique structural characters at the highest elevation showed a tendency to form their own haplotype cluster, as revealed by the COII gene. Tajima's D, Fu's Fs, and mismatch distribution tests revealed population expansion of S. tani in Cameron Highlands. A strong correlation was found between nucleotide diversity and the levels of dissolved oxygen in the streams where S. tani was collected.
    MeSH terms: Altitude; Amino Acid Sequence; Animals; Electron Transport Complex IV/genetics*; DNA, Mitochondrial/genetics*; Genetic Markers; Genetics, Population; Haplotypes; Malaysia; Mitochondria/genetics; Molecular Sequence Data; Phylogeny*; Simuliidae/classification; Simuliidae/genetics*; Genetic Variation; Ecosystem; Protein Subunits/genetics*; Gene Flow
  2. Jefferies JM, Mohd Yusof MY, Devi Sekaran S, Clarke SC
    PLoS One, 2014;9(6):e97912.
    PMID: 24941079 DOI: 10.1371/journal.pone.0097912
    Although Streptococcus pneumoniae is a leading cause of childhood disease in South East Asia, little has previously been reported regarding the epidemiology of invasive pneumococcal disease in Malaysia and very few studies have explored pneumococcal epidemiology using multilocus sequence typing (MLST). Here we describe serotype, multilocus sequence type (ST), and penicillin susceptibility of thirty pneumococcal invasive disease isolates received by the University of Malaya Medical Centre between February 2000 and January 2007 and relate this to the serotypes included in current pneumococcal conjugate vaccines. A high level of diversity was observed; fourteen serotypes and 26 sequence types (ST), (11 of which were not previously described) were detected from 30 isolates. Penicillin non-susceptible pneumococci accounted for 33% of isolates. The extent of molecular heterogeneity within carried and disease-causing Malaysian pneumococci remains unknown. Larger surveillance and epidemiological studies are now required in this region to provide robust evidence on which to base future vaccine policy.
    MeSH terms: Adolescent; Adult; Aged; Aged, 80 and over; Anti-Bacterial Agents/therapeutic use; Child; Child, Preschool; Clone Cells; Humans; Infant; Infant, Newborn; Malaysia/epidemiology; Middle Aged; Penicillins/therapeutic use; Pneumococcal Infections/drug therapy; Pneumococcal Infections/microbiology; Pneumococcal Infections/epidemiology*; Pneumococcal Infections/prevention & control*; Serotyping; Streptococcus pneumoniae/classification*; Streptococcus pneumoniae/genetics*; Streptococcus pneumoniae/isolation & purification; Vaccines, Conjugate; beta-Lactam Resistance; Pneumococcal Vaccines/administration & dosage*; Pneumococcal Vaccines/chemistry; Multilocus Sequence Typing
  3. Barbour A, Philip K
    PLoS One, 2014;9(6):e100541.
    PMID: 24941127 DOI: 10.1371/journal.pone.0100541
    Salivaricins are bacteriocins produced by Streptococcus salivarius, some strains of which can have significant probiotic effects. S. salivarius strains were isolated from Malaysian subjects showing variable antimicrobial activity, metabolic profile, antibiotic susceptibility and lantibiotic production.
    MeSH terms: Aerobiosis; Amino Acid Sequence; Bacterial Proteins/biosynthesis; Bacterial Proteins/isolation & purification; Bacteriocins/biosynthesis; Bacteriocins/isolation & purification*; Culture Media/pharmacology; Culture Media/chemistry; Genes, Bacterial*; Hexosyltransferases/biosynthesis; Hexosyltransferases/isolation & purification*; Humans; Malaysia; Molecular Sequence Data; Morpholines/chemistry; Streptococcus/drug effects; Streptococcus/genetics; Streptococcus/isolation & purification; Streptococcus/metabolism*; Gene Expression; Alkanesulfonic Acids/chemistry; Probiotics
  4. Subramaniam S, Sabaratnam V, Kuppusamy UR, Tan YS
    Int J Med Mushrooms, 2014;16(3):259-67.
    PMID: 24941167
    Species of the genus Ganoderma are a cosmopolitan wood decaying white rot fungi, which has been used by the Asians for therapeutic purposes for centuries. In the present study, solid-substrate fermentation (SSF) of wheat grains (Triticum aestivum L.) was carried out with indigenous Ganoderma australe (KUM60813) and G. neo-japonicum (KUM61076) selected based on ethnomycological knowledge. G. lucidum (VITA GL) (a commercial strain) was also included in the study. Antioxidant activities of the crude ethanol and aqueous extracts of the fermented and unfermented wheat grains were investigated by ferric reducing antioxidant power (FRAP), Trolox equivalent antioxidant capacity (TEAC), diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging ability, and lipid peroxidation assay. Among the six mycelia extracts tested, the ethanol extract from wheat fermented with KUM61076 mycelia showed the most potent antioxidant activities, whereas the ethanol extract of wheat grains fermented with KUM60813 mycelia has a good potential in protecting frying oils against oxidation. Total phenolic content (TPC) in the ethanol extracts were higher than that in the aqueous extract. The wheat grains fermented with G. australe (KUM60813) and G. neo-japonicum KUM61076 have greater antioxidant potential compared to the commercially available G. lucidum (VITA GL). The antioxidant activities of the mycelia extracts had a positive correlation with their phenolic contents. Thus phenolic compounds may play a vital role in the antioxidant activities of the selected Ganoderma spp.
    MeSH terms: Antioxidants/isolation & purification; Antioxidants/metabolism; Antioxidants/chemistry*; Culture Media/metabolism; Fermentation; Plant Extracts/isolation & purification; Plant Extracts/metabolism; Plant Extracts/chemistry*; Vegetables/chemistry*; Triticum/metabolism; Triticum/microbiology*; Mycelium/growth & development; Mycelium/metabolism; Mycelium/chemistry*; Ganoderma/growth & development*; Ganoderma/metabolism; Ganoderma/chemistry
  5. Cros A, Ahamad Fatan N, White A, Teoh SJ, Tan S, Handayani C, et al.
    PLoS One, 2014;9(6):e96332.
    PMID: 24941442 DOI: 10.1371/journal.pone.0096332
    In this paper we describe the construction of an online GIS database system, hosted by WorldFish, which stores bio-physical, ecological and socio-economic data for the 'Coral Triangle Area' in South-east Asia and the Pacific. The database has been built in partnership with all six (Timor-Leste, Malaysia, Indonesia, The Philippines, Solomon Islands and Papua New Guinea) of the Coral Triangle countries, and represents a valuable source of information for natural resource managers at the regional scale. Its utility is demonstrated using biophysical data, data summarising marine habitats, and data describing the extent of marine protected areas in the region.
    MeSH terms: Animals; Asia, Southeastern; Atlases as Topic; Conservation of Natural Resources; Environmental Monitoring*; Humans; Pacific Ocean; Databases, Factual*; Internet; Anthozoa/physiology*; Geographic Information Systems; Coral Reefs*
  6. Nikolov LA, Tomlinson PB, Manickam S, Endress PK, Kramer EM, Davis CC
    Ann Bot, 2014 Aug;114(2):233-42.
    PMID: 24942001 DOI: 10.1093/aob/mcu114
    Species in the holoparasitic plant family Rafflesiaceae exhibit one of the most highly modified vegetative bodies in flowering plants. Apart from the flower shoot and associated bracts, the parasite is a mycelium-like endophyte living inside their grapevine hosts. This study provides a comprehensive treatment of the endophytic vegetative body for all three genera of Rafflesiaceae (Rafflesia, Rhizanthes and Sapria), and reports on the cytology and development of the endophyte, including its structural connection to the host, shedding light on the poorly understood nature of this symbiosis.
    MeSH terms: Plant Roots/anatomy & histology; Plant Roots/microbiology; Plant Shoots/microbiology; Angiosperms/anatomy & histology*; Angiosperms/microbiology*; Flowers/anatomy & histology*; Endophytes/cytology; Endophytes/physiology*
  7. Prow NA, Setoh YX, Biron RM, Sester DP, Kim KS, Hobson-Peters J, et al.
    J Virol, 2014 Sep 1;88(17):9947-62.
    PMID: 24942584 DOI: 10.1128/JVI.01304-14
    The mosquito-borne West Nile virus (WNV) is responsible for outbreaks of viral encephalitis in humans, horses, and birds, with particularly virulent strains causing recent outbreaks of disease in eastern Europe, the Middle East, North America, and Australia. Previous studies have phylogenetically separated WNV strains into two main genetic lineages (I and II) containing virulent strains associated with neurological disease. Several WNV-like strains clustering outside these lineages have been identified and form an additional five proposed lineages. However, little is known about whether these strains have the potential to induce disease. In a comparative analysis with the highly virulent lineage I American strain (WNVNY99), the low-pathogenicity lineage II strain (B956), a benign Australian strain, Kunjin (WNVKUN), the African WNV-like Koutango virus (WNVKOU), and a WNV-like isolate from Sarawak, Malaysia (WNVSarawak), were assessed for neuroinvasive properties in a murine model and for their replication kinetics in vitro. While WNVNY99 replicated to the highest levels in vitro, in vivo mouse challenge revealed that WNVKOU was more virulent, with a shorter time to onset of neurological disease and higher morbidity. Histological analysis of WNVKOU- and WNVNY99-infected brain and spinal cords demonstrated more prominent meningoencephalitis and the presence of viral antigen in WNVKOU-infected mice. Enhanced virulence of WNVKOU also was associated with poor viral clearance in the periphery (sera and spleen), a skewed innate immune response, and poor neutralizing antibody development. These data demonstrate, for the first time, potent neuroinvasive and neurovirulent properties of a WNV-like virus outside lineages I and II.
    MeSH terms: Animals; Antibodies, Viral/blood*; Brain/pathology; Brain/virology; Disease Models, Animal; Encephalitis, Arbovirus/immunology; Encephalitis, Arbovirus/pathology*; Encephalitis, Arbovirus/virology; Spinal Cord/pathology; Spinal Cord/virology; Virulence; Virus Replication; Survival Analysis; Flavivirus Infections/immunology; Flavivirus Infections/pathology*; Flavivirus Infections/virology; Encephalitis Viruses, Japanese/immunology*; Encephalitis Viruses, Japanese/pathogenicity*; Mice; Antibodies, Neutralizing/blood*
  8. Hertwig ST, Min PY, Haas A, Das I
    Zootaxa, 2014.
    PMID: 24943439 DOI: 10.11646/zootaxa.3814.3.9
    A new species of stream toad of the genus Ansonia is described from Gunung Murud, Pulong Tau National Park, of northern Sarawak, Malaysia, Borneo. Ansonia vidua, sp. nov., is morphologically distinguished from its Bornean congeners by the following combination of characters: medium size (SVL of adult females 33.5-34.4 mm); body uniformly black-brown in life; absence of a visible pattern on dorsum or limbs; presence of two low interorbital ridges; shagreened skin on dorsum, sides and upper surfaces of the limbs with numerous homogeneously small, rounded warts; first finger shorter than second; reduced webbing between the toes and an absence of a sharp tarsal ridge. Uncorrected genetic distances between related taxa of > 4.3% in 16S rRNA gene support its status as a hitherto undescribed species.
    MeSH terms: Animals; Animal Structures/anatomy & histology; Bufonidae/anatomy & histology; Bufonidae/classification*; Bufonidae/genetics; Female; Malaysia; Male; Molecular Sequence Data; Phylogeny; Ecosystem; Animal Distribution
  9. Milne RL, Burwinkel B, Michailidou K, Arias-Perez JI, Zamora MP, Menéndez-Rodríguez P, et al.
    Hum Mol Genet, 2014 Nov 15;23(22):6096-111.
    PMID: 24943594 DOI: 10.1093/hmg/ddu311
    Candidate variant association studies have been largely unsuccessful in identifying common breast cancer susceptibility variants, although most studies have been underpowered to detect associations of a realistic magnitude. We assessed 41 common non-synonymous single-nucleotide polymorphisms (nsSNPs) for which evidence of association with breast cancer risk had been previously reported. Case-control data were combined from 38 studies of white European women (46 450 cases and 42 600 controls) and analyzed using unconditional logistic regression. Strong evidence of association was observed for three nsSNPs: ATXN7-K264R at 3p21 [rs1053338, per allele OR = 1.07, 95% confidence interval (CI) = 1.04-1.10, P = 2.9 × 10(-6)], AKAP9-M463I at 7q21 (rs6964587, OR = 1.05, 95% CI = 1.03-1.07, P = 1.7 × 10(-6)) and NEK10-L513S at 3p24 (rs10510592, OR = 1.10, 95% CI = 1.07-1.12, P = 5.1 × 10(-17)). The first two associations reached genome-wide statistical significance in a combined analysis of available data, including independent data from nine genome-wide association studies (GWASs): for ATXN7-K264R, OR = 1.07 (95% CI = 1.05-1.10, P = 1.0 × 10(-8)); for AKAP9-M463I, OR = 1.05 (95% CI = 1.04-1.07, P = 2.0 × 10(-10)). Further analysis of other common variants in these two regions suggested that intronic SNPs nearby are more strongly associated with disease risk. We have thus identified a novel susceptibility locus at 3p21, and confirmed previous suggestive evidence that rs6964587 at 7q21 is associated with risk. The third locus, rs10510592, is located in an established breast cancer susceptibility region; the association was substantially attenuated after adjustment for the known GWAS hit. Thus, each of the associated nsSNPs is likely to be a marker for another, non-coding, variant causally related to breast cancer risk. Further fine-mapping and functional studies are required to identify the underlying risk-modifying variants and the genes through which they act.
    MeSH terms: Ataxin-7; Adult; Alleles; Breast Neoplasms/genetics*; Cytoskeletal Proteins/genetics; Female; Humans; Middle Aged; Nerve Tissue Proteins/genetics; Case-Control Studies; Protein-Serine-Threonine Kinases/genetics; Genetic Predisposition to Disease*; Polymorphism, Single Nucleotide*; A Kinase Anchor Proteins/genetics; Genome-Wide Association Study; NIMA-Related Kinases
  10. Grismer LL, Quah ES, Anuar M S S, Muin MA, Wood PL, Nor SA
    Zootaxa, 2014.
    PMID: 24943599 DOI: 10.11646/zootaxa.3815.1.3
    A newly discovered, diminutive, cave-dwelling, lowland species of the colubrid snake genus Lycodon Boie is described from a limestone cave along the Thai-Malaysian border in the state of Perlis, northwestern Peninsular Malaysia. Lycodon cavernicolus sp. nov. is most closely related to L. butleri Boulenger, an endemic, upland, forest-dwelling species from Peninsular Malaysia of the fasciatus group but is separated from L. butleri and all other species of the L. fasciatus group and the closely related L. ruhstrati group by having the combination of 245 (male) and 232 (female) ventral scales; 113 (male) and 92 (female) paired, subcaudal scales; a single precloacal plate; nine or 10 supralabials; 10 or 11 infralabials; a maximum total length of 508 mm (female); a relative tail length of 0.25-0.27; an immaculate venter in juveniles and dark-brown, posterior, ventral scale margins in adults; and dorsal and caudal bands in juveniles white. The discovery of L. cavernicolus sp. nov. adds to a rapidly growing list of newly discovered reptiles from karst regions and limestone forests of Peninsular Malaysia, underscoring the fact that these areas should be studied before they are quarried as they harbor a significant portion of the Peninsular Malaysia's herpetological diversity.
    MeSH terms: Animals; Female; Malaysia; Male; Molecular Sequence Data; Phylogeny; Ecosystem; Colubridae/anatomy & histology; Colubridae/classification*; Colubridae/genetics; Reptilian Proteins/genetics; Caves
  11. Yin ZW, Li LZ
    Zootaxa, 2014.
    PMID: 24943626 DOI: 10.11646/zootaxa.3815.3.9
    A new species, Megatyrus femoralis sp. n., is described from the Koshi Zone, East Nepal, with major diagnostic features illustrated. Megatyrus masumotoi Nomura, Sakchoowong & Chanpaisaeng, originally described from southwestern Thailand, is recorded from the Noring Timur Mountain, West Malaysia. The above data extends the known range of Megatyrus about 1,200 km to the west, and 870 km to the south.
    MeSH terms: Animals; Animal Structures/anatomy & histology; Asia; Beetles/anatomy & histology; Beetles/classification*; Female; Male; Animal Distribution
  12. Väisänen R
    Zootaxa, 2014.
    PMID: 24943632 DOI: 10.11646/zootaxa.3815.4.4
    Mycomya Rondani specimens from the islands of South-East Asia, i.e. Malaysia, Indonesia and the Philippines, are revised. The paper includes a key to the Mycomya species of the South-East Asian islands. The following six new species are described: M. shimai sp. n. from Java, Indonesia, M. pongo sp. n. from Sabah, Malaysia, and M. apoensis sp. n., M. nakanishii sp. n., M. paraklossi sp. n. and M. yatai sp. n. from Mindanao, the Philippines. The holotypes of M. klossi Edwards from Borneo, Malaysia, and M. minutata Edwards from Sumatra, Indonesia, were examined and their genitalia are described. M. occultans (Winnertz) is recorded from Java, Indonesia.
    MeSH terms: Animals; Animal Structures/anatomy & histology; Asia, Southeastern; Diptera/anatomy & histology; Diptera/classification*; Female; Male; Animal Distribution
  13. Chai PF, Rathinam X, Solayappan M, Ahmad Ghazali AH, Subramaniam S
    Microscopy (Oxf), 2014 Oct;63(5):371-5.
    PMID: 24943903 DOI: 10.1093/jmicro/dfu022
    The current study focused on the microscopic studies of a native Bacillus thuringiensis strain isolated from Malaysia, Bt-S84-13a, that produced an unusual crystal type. Primary detection of parasporal inclusions using a phase contrast microscope presented one to two small crystal proteins in the sporulating cells of Bt-S84-13a. Compound light microscopic examination of autolysed Bt-S84-13a cells stained with 0.133% Coomassie Brilliant Blue showed two types of crystal morphology: small crystals independent of spores and spore-associated crystals. Surface structure analysis with a scanning electron microscope revealed spherical-like, coarse and wrinkled-looking crystal in Bt-S84-13a. A close-up observation of the crystal morphology using a transmission electron microscope also demonstrated two parasporal inclusions in Bt-S84-13a. One inclusion was deposited against the forespore and was in a shape of incomplete rectangular. Another smaller inclusion was developed within the exosporium and was rectangular in shape. However, the latter inclusion was found lack in another bacterial cell which was still in the early stages of sporulation. This unique crystal morphology may imply some biological potential in Bt-S84-13a.
    MeSH terms: Bacillus thuringiensis/physiology; Bacillus thuringiensis/ultrastructure*; Bacterial Proteins/ultrastructure; Crystallization; Endotoxins; Hemolysin Proteins/ultrastructure; Malaysia; Microscopy, Electron, Scanning; Microscopy, Phase-Contrast; Spores, Bacterial/ultrastructure
  14. Ng KT, Ng KY, Chen JH, Ng OT, Kamarulzaman A, Tee KK
    Clin Infect Dis, 2014 Sep 15;59(6):910-1.
    PMID: 24944233 DOI: 10.1093/cid/ciu480
    MeSH terms: Asia; Humans; Male; HIV-1*; HIV Infections/epidemiology; HIV Infections/transmission*; Homosexuality, Male*
  15. Komahan K, Reidpath DD
    Am J Epidemiol, 2014 Aug 1;180(3):325-9.
    PMID: 24944286 DOI: 10.1093/aje/kwu129
    Correct identification of ethnicity is central to many epidemiologic analyses. Unfortunately, ethnicity data are often missing. Successful classification typically relies on large databases (n > 500,000 names) of known name-ethnicity associations. We propose an alternative naïve Bayesian strategy that uses substrings of full names. Name and ethnicity data for Malays, Indians, and Chinese were provided by a health and demographic surveillance site operating in Malaysia from 2011-2013. The data comprised a training data set (n = 10,104) and a test data set (n = 9,992). Names were spliced into contiguous 3-letter substrings, and these were used as the basis for the Bayesian analysis. Performance was evaluated on both data sets using Cohen's κ and measures of sensitivity and specificity. There was little difference between the classification performance in the training and test data (κ = 0.93 and 0.94, respectively). For the test data, the sensitivity values for the Malay, Indian, and Chinese names were 0.997, 0.855, and 0.932, respectively, and the specificity values were 0.907, 0.998, and 0.997, respectively. A naïve Bayesian strategy for the classification of ethnicity is promising. It performs at least as well as more sophisticated approaches. The possible application to smaller data sets is particularly appealing. Further research examining other substring lengths and other ethnic groups is warranted.
    MeSH terms: Bayes Theorem*; China; Ethnic Groups*; Humans; India; Malaysia; Names*; European Continental Ancestry Group; Asian Continental Ancestry Group
  16. Paydar M, Kamalidehghan B, Wong YL, Wong WF, Looi CY, Mustafa MR
    Drug Des Devel Ther, 2014;8:719-33.
    PMID: 24944509 DOI: 10.2147/DDDT.S58178
    To date, plants have been the major source of anticancer drugs. Boldine is a natural alkaloid commonly found in the leaves and bark of Peumus boldus. In this study, we found that boldine potently inhibited the viability of the human invasive breast cancer cell lines, MDA-MB-231 (48-hour IC₅₀ 46.5±3.1 μg/mL) and MDA-MB-468 (48-hour IC₅₀ 50.8±2.7 μg/mL). Boldine had a cytotoxic effect and induced apoptosis in breast cancer cells as indicated by a higher amount of lactate dehydrogenase released, membrane permeability, and DNA fragmentation. In addition, we demonstrated that boldine induced cell cycle arrest at G2/M phase. The anticancer mechanism is associated with disruption of the mitochondrial membrane potential and release of cytochrome c in MDA-MB-231. Boldine selectively induced activation of caspase-9 and caspase-3/7, but not caspase-8. We also found that boldine could inhibit nuclear factor kappa B activation, a key molecule in tumor progression and metastasis. In addition, protein array and Western blotting analysis showed that treatment with boldine resulted in downregulation of Bcl-2 and heat shock protein 70 and upregulation of Bax in the MDA-MB-231 cell line. An acute toxicity study in rats revealed that boldine at a dose of 100 mg/kg body weight was well tolerated. Moreover, intraperitoneal injection of boldine (50 or 100 mg/kg) significantly reduced tumor size in an animal model of breast cancer. Our results suggest that boldine is a potentially useful agent for the treatment of breast cancer.
    MeSH terms: Animals; Antineoplastic Combined Chemotherapy Protocols/pharmacology*; Antineoplastic Combined Chemotherapy Protocols/chemistry; Aporphines/pharmacology*; Aporphines/chemistry; Breast Neoplasms/drug therapy*; Breast Neoplasms/metabolism; Breast Neoplasms/pathology; Cell Survival/drug effects; Disease Models, Animal*; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; Female; Humans; L-Lactate Dehydrogenase/metabolism; Structure-Activity Relationship; Tumor Cells, Cultured; Molecular Structure; Apoptosis/drug effects; Cell Proliferation/drug effects; Rats
  17. Chee Yen W, Mohd Shariff Z, Kandiah M, Mohd Taib MN
    Nutr Res Pract, 2014 Jun;8(3):297-303.
    PMID: 24944775 DOI: 10.4162/nrp.2014.8.3.297
    BACKGROUND/OBJECTIVES: Understanding individual's intention, action and maintenance to increase fruit and vegetable intake is an initial step in designing nutrition or health promotion programs. This study aimed to determine stages of change to increase fruit and vegetable intake and its relationships with fruit and vegetable intake, self-efficacy, perceived benefits and perceived barriers.

    SUBJECTS/METHODS: This cross-sectional study was conducted among 348 public university staff in Universiti Putra Malaysia. A pre-tested self-administered questionnaire and two days 24-hour diet recall were used.

    RESULTS: Half of the respondents (50%) were in preparation stage, followed by 43% in action/maintenance, 7% in pre-contemplation/contemplation stages. Respondents in action/maintenance stages had significantly higher self-efficacy (F = 9.17, P < 0.001) and perceived benefits (F = 5.07, P < 0.01) while respondents in pre-contemplation/contemplation and preparation stages had significantly higher perceived barriers (F = 4.83, P < 0.05). Perceived benefits tend to outweigh perceived barriers pre-ceding to taking action. Self-efficacy is important in motivating individuals to increase fruit and vegetable intake as self-efficacy and perceived barriers crossed over between preparation and action/maintenance. Respondents in action/maintenance stages had the highest adjusted mean serving of fruit and vegetable intake (F = 4.52, P < 0.05) but the intake did not meet recommendation.

    CONCLUSION: Intervention strategies should emphasize on increasing perceived benefits and building self-efficacy by providing knowledge and skills to consume a diet high in fruits and vegetables in order to promote healthy changes in having high fruit and vegetable intake.

  18. Ali Naser I, Jalil R, Wan Muda WM, Wan Nik WS, Mohd Shariff Z, Abdullah MR
    Nutr Res Pract, 2014 Jun;8(3):304-11.
    PMID: 24944776 DOI: 10.4162/nrp.2014.8.3.304
    BACKGROUND/OBJECTIVES: The purpose of the study was to investigate the relationship between household food insecurity and nutritional status of children in low-income households. A cross sectional study involved a survey of households (n = 223) receiving the financial assistance.
    SUBJECTS/METHODS: Eligible mothers that fulfilled the inclusion criteria such as non-pregnant, non-lactating mothers, aged 18 to 55 years with their youngest children aged 2 to 12 years, were purposively selected. The Radimer/Cornell hunger and food-insecurity instrument was administered and children's height and weight were measured.
    RESULTS: About 16.1% of the households were food secure, while 83.9% experienced some kind of food insecurity. Out of food insecure category, 29.6% households were food insecure, 19.3% women were individual food insecure and 35.0% fell into the child hunger category. Education of the mother (P = 0.047), household size (P = 0.024), number of children (P = 0.024), number of children going to school (P = 0.048), total monthly income (P < 0.001), income per capital (P < 0.001), number of household members contributing to the income (P = 0.018) and food expenditure (P = 0.006) were significant risk factors for household food insecurity. The prevalence of underweight, stunting and wasting in children were 61.0%, 61.4% and 30.6% respectively. Based on multinomial logistic regression, children in food-insecure households were 2.15 times more likely to be underweight and three times to be stunted than children in the food-secure households.
    CONCLUSIONS: The findings suggest that household food insecurity is associated with the nutritional status of the children in the rural area of Northeastern Peninsular Malaysia.
    KEYWORDS: Northeastern Peninsular Malaysia; child hunger; household food insecurity; nutritional status
    MeSH terms: Adolescent; Adult; Body Weight; Child; Child, Preschool; Cross-Sectional Studies; Health Expenditures; Female; Food Supply; Growth Disorders; Humans; Hunger; Income; Malaysia; Male; Middle Aged; Mothers; Nutritional Status; Surveys and Questionnaires; Risk Factors; Thinness; Prevalence; Logistic Models; Young Adult
  19. Subramani B, Pullai CR, Krishnan K, Sugadan SD, Deng X, Hiroshi T, et al.
    Biomed Rep, 2014 Jul;2(4):505-508.
    PMID: 24944796
    Immune cell-based therapies using natural killer (NK) cells and cytotoxic T cells are under constant scrutiny, with the aim to design an effective and reduced-toxicity therapy, which will benefit patients via improved quality of life and improved prognosis. Four patients with stage IV colon cancer were administered 1, 3, 5 and 6 effector cell intravenous infusions, respectively. Peripheral blood was collected from the patients and the ex vivo activation and expansion of NK and T cells was performed in Good Manufacturing Practice-certified clean rooms for ~12-15 days. Immunophenotypic analysis of the peripheral blood mononuclear cells (PBMCs) and expanded NK and T cells was conducted using flow cytometry and the patients were followed up. On average, 4.8×107 initial PBMCs and 2.7×109 total expanded cells were obtained. The intravenous infusions of the expanded cells were not accompanied by adverse reactions. Improved prognosis, reflected by a considerable decrease in the cancer markers, accompanied by an improved quality of life in the patients were observed. In conclusion, potential strategies are currently under development for the large-scale production of effectors cells; therefore, autologous immune enhancement therapy (AIET) may be considered as a viable approach to cancer treatment.
    MeSH terms: Colonic Neoplasms; Environment, Controlled; Flow Cytometry; Humans; Infusions, Intravenous; Killer Cells, Natural; Leukocytes, Mononuclear; Prognosis; Quality of Life; T-Lymphocytes, Cytotoxic; Immunophenotyping
  20. Ibahim MJ, Crosbie JC, Yang Y, Zaitseva M, Stevenson AW, Rogers PA, et al.
    PLoS One, 2014;9(6):e100547.
    PMID: 24945301 DOI: 10.1371/journal.pone.0100547
    High-dose synchrotron microbeam radiation therapy (MRT) has shown the potential to deliver improved outcomes over conventional broadbeam (BB) radiation therapy. To implement synchrotron MRT clinically for cancer treatment, it is necessary to undertake dose equivalence studies to identify MRT doses that give similar outcomes to BB treatments.
    MeSH terms: Animals; Australia; Biological Assay/methods*; Cell Survival/radiation effects; Clone Cells; Dose-Response Relationship, Radiation; Therapeutic Equivalency; Electric Impedance; Synchrotrons*; Cell Line, Tumor; Cell Proliferation/radiation effects; Mice
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