Browse publications by year: 2014

  1. Parasuraman S, Thing GS, Dhanaraj SA
    Pharmacogn Rev, 2014 Jul;8(16):73-80.
    PMID: 25125878 DOI: 10.4103/0973-7847.134229
    Ayurveda is one of the traditional medicinal systems of Indian. The philosophy behind Ayurveda is preventing unnecessary suffering and living a long healthy life. Ayurveda involves the use of natural elements to eliminate the root cause of the disease by restoring balance, at the same time create a healthy life-style to prevent the recurrence of imbalance. Herbal medicines have existed world-wide with long recorded history and they were used in ancient Chinese, Greek, Egyptian and Indian medicine for various therapies purposes. World Health Organization estimated that 80% of the word's inhabitants still rely mainly on traditional medicines for their health care. The subcontinent of India is well-known to be one of the major biodiversity centers with about 45,000 plant species. In India, about 15,000 medicinal plants have been recorded, in which the communities used 7,000-7,500 plants for curing different diseases. In Ayurveda, single or multiple herbs (polyherbal) are used for the treatment. The Ayurvedic literature Sarangdhar Samhita' highlighted the concept of polyherbalism to achieve greater therapeutic efficacy. The active phytochemical constituents of individual plants are insufficient to achieve the desirable therapeutic effects. When combining the multiple herbs in a particular ratio, it will give a better therapeutic effect and reduce the toxicity. This review mainly focuses on important of the polyherbalism and its clinical significance.
    MeSH terms: Egypt; Greece; India; Medicine, Ayurvedic; Medicine, Traditional; Plants, Medicinal; Herbal Medicine; Biodiversity; Phytochemicals
  2. Siddiqui MJ, Min CS, Verma RK, Jamshed SQ
    Pharmacogn Rev, 2014 Jul;8(16):81-7.
    PMID: 25125879 DOI: 10.4103/0973-7847.134230
    Since time immemorial homo sapiens are subjected to both health and diseases states and seek treatment for succor and assuagement in compromised health states. Since last two decades the progressive rise in the alternative form of treatment cannot be ignored and population seems to be dissatisfied with the conventional treatment modalities and therefore, resort to other forms of treatment, mainly complementary and alternative medicine (CAM). The use of CAM is predominantly more popular in older adults and therefore, numerous research studies and clinical trials have been carried out to investigate the effectiveness of CAM in the management of both communicable and non-communicable disease. In this current mini review, we attempt to encompass the use of CAM in chronic non-communicable diseases that are most likely seen in geriatrics. The current review focuses not only on the reassurance of good health practices, emphasizing on the holistic development and strengthening the body's defense mechanisms, but also attempts to construct a pattern of self-care and patient empowerment in geriatrics. The issues of safety with CAM use cannot be sidelined and consultation with a health care professional is always advocated to the patient. Likewise, responsibility of the health care professional is to inform the patient about the safety and efficacy issues. In order to substantiate the efficacy and safety of CAMs, evidence-based studies and practices with consolidated standards should be planned and executed.
  3. Reidpath DD, Ahmadi K
    PMID: 25126103 DOI: 10.1186/1742-7622-11-9
    BACKGROUND: Measures of household socio-economic position (SEP) are widely used in health research. There exist a number of approaches to their measurement, with Principal Components Analysis (PCA) applied to a basket of household assets being one of the most common. PCA, however, carries a number of assumptions about the distribution of the data which may be untenable, and alternative, non-parametric, approaches may be preferred. Mokken scale analysis is a non-parametric, item response theory approach to scale development which appears never to have been applied to household asset data. A Mokken scale can be used to rank order items (measures of wealth) as well as households. Using data on household asset ownership from a national sample of 4,154 consenting households in the World Health Survey from Vietnam, 2003, we construct two measures of household SEP. Seventeen items asking about assets, and utility and infrastructure use were used. Mokken Scaling and PCA were applied to the data. A single item measure of total household expenditure is used as a point of contrast.

    RESULTS: An 11 item scale, out of the 17 items, was identified that conformed to the assumptions of a Mokken Scale. All the items in the scale were identified as strong items (Hi > .5). Two PCA measures of SEP were developed as a point of contrast. One PCA measure was developed using all 17 available asset items, the other used the reduced set of 11 items identified in the Mokken scale analaysis. The Mokken Scale measure of SEP and the 17 item PCA measure had a very high correlation (r = .98), and they both correlated moderately with total household expenditure: r = .59 and r = .57 respectively. In contrast the 11 item PCA measure correlated moderately with the Mokken scale (r = .68), and weakly with the total household expenditure (r = .18).

    CONCLUSION: The Mokken scale measure of household SEP performed at least as well as PCA, and outperformed the PCA measure developed with the 11 items used in the Mokken scale. Unlike PCA, Mokken scaling carries no assumptions about the underlying shape of the distribution of the data, and can be used simultaneous to order household SEP and items. The approach, however, has not been tested with data from other countries and remains an interesting, but under researched approach.

  4. Ramimoghadam D, Bagheri S, Abd Hamid SB
    Biomed Res Int, 2014;2014:205636.
    PMID: 25126547 DOI: 10.1155/2014/205636
    Anatase titanium dioxide nanoparticles (TiO2-NPs) were synthesized by sol-gel method using rice straw as a soft biotemplate. Rice straw, as a lignocellulosic waste material, is a biomass feedstock which is globally produced in high rate and could be utilized in an innovative approach to manufacture a value-added product. Rice straw as a reliable biotemplate has been used in the sol-gel method to synthesize ultrasmall sizes of TiO2-NPs with high potential application in photocatalysis. The physicochemical properties of titanium dioxide nanoparticles were investigated by a number of techniques such as X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), ultraviolet visible spectra (UV-Vis), and surface area and pore size analysis. All results consensually confirmed that particle sizes of synthesized titanium dioxide were template-dependent, representing decrease in the nanoparticles sizes with increase of biotemplate concentration. Titanium dioxide nanoparticles as small as 13.0 ± 3.3 nm were obtained under our experimental conditions. Additionally, surface area and porosity of synthesized TiO2-NPs have been enhanced by increasing rice straw amount which results in surface modification of nanoparticles and potential application in photocatalysis.
    MeSH terms: Lignin/chemical synthesis; Lignin/chemistry; Particle Size; Oryza/chemistry; Spectrum Analysis, Raman; Titanium/chemistry*; X-Ray Diffraction; Spectroscopy, Fourier Transform Infrared; Microscopy, Electron, Transmission; Metal Nanoparticles/ultrastructure; Metal Nanoparticles/chemistry*; Solid Waste*
  5. Ahmed Hamdi OA, Syed Abdul Rahman SN, Awang K, Abdul Wahab N, Looi CY, Thomas NF, et al.
    ScientificWorldJournal, 2014;2014:321943.
    PMID: 25126594 DOI: 10.1155/2014/321943
    Curcuma zedoaria also known as Temu putih is traditionally used in food preparations and treatment of various ailments including cancer. The cytotoxic activity of hexane, dichloromethane, ethyl acetate, methanol, and the methanol-soxhlet extracts of Curcuma zedoaria rhizomes was tested on two human cancer cell lines (Ca Ski and MCF-7) and a noncancer cell line (HUVEC) using MTT assay. Investigation on the chemical components in the hexane and dichloromethane fractions gave 19 compounds, namely, labda-8(17),12 diene-15,16 dial (1), dehydrocurdione (2), curcumenone (3), comosone II (4), curcumenol (5), procurcumenol (6), germacrone (7), zerumbone epoxide (8), zederone (9), 9-isopropylidene-2,6-dimethyl-11-oxatricyclo[6.2.1.0(1,5)]undec-6-en-8-ol (10), furanodiene (11), germacrone-4,5-epoxide (12), calcaratarin A (13), isoprocurcumenol (14), germacrone-1,10-epoxide (15), zerumin A (16), curcumanolide A (17), curcuzedoalide (18), and gweicurculactone (19). Compounds (1-19) were evaluated for their antiproliferative effect using MTT assay against four cancer cell lines (Ca Ski, MCF-7, PC-3, and HT-29). Curcumenone (3) and curcumenol (5) displayed strong antiproliferative activity (IC50 = 8.3 ± 1.0 and 9.3 ± 0.3 μg/mL, resp.) and were found to induce apoptotic cell death on MCF-7 cells using phase contrast and Hoechst 33342/PI double-staining assay. Thus, the present study provides basis for the ethnomedical application of Curcuma zedoaria in the treatment of breast cancer.
    MeSH terms: Analysis of Variance; Breast Neoplasms/drug therapy*; Chromatography, Thin Layer; Female; Humans; Indonesia; Malaysia; Phytotherapy/methods*; Microscopy, Fluorescence; Plant Extracts/pharmacology*; Plant Extracts/therapeutic use; Plant Extracts/chemistry; Tetrazolium Salts; Thiazoles; Molecular Structure; Rhizome/chemistry*; Curcuma/chemistry*; Cell Proliferation/drug effects*; Human Umbilical Vein Endothelial Cells; MCF-7 Cells
  6. Firoozi AA, Taha MR, Mir Moammad Hosseini SM, Firoozi AA
    ScientificWorldJournal, 2014;2014:325759.
    PMID: 25126595 DOI: 10.1155/2014/325759
    Deformation of quay walls is one of the main sources of damage to port facility while liquefaction of backfill and base soil of the wall are the main reasons for failures of quay walls. During earthquakes, the most susceptible materials for liquefaction in seashore regions are loose saturated sand. In this study, effects of enhancing the wall width and the soil improvement on the behavior of gravity quay walls are examined in order to obtain the optimum improved region. The FLAC 2D software was used for analyzing and modeling progressed models of soil and loading under difference conditions. Also, the behavior of liquefiable soil is simulated by the use of "Finn" constitutive model in the analysis models. The "Finn" constitutive model is especially created to determine liquefaction phenomena and excess pore pressure generation.
    MeSH terms: Gravitation*; Models, Theoretical*; Software*; Soil/chemistry*; Earthquakes*; Structure Collapse/prevention & control*
  7. Yakubu ML, Yusop Z, Yusof F
    ScientificWorldJournal, 2014;2014:361703.
    PMID: 25126597 DOI: 10.1155/2014/361703
    This paper presents the modelled raindrop size parameters in Skudai region of the Johor Bahru, western Malaysia. Presently, there is no model to forecast the characteristics of DSD in Malaysia, and this has an underpinning implication on wet weather pollution predictions. The climate of Skudai exhibits local variability in regional scale. This study established five different parametric expressions describing the rain rate of Skudai; these models are idiosyncratic to the climate of the region. Sophisticated equipment that converts sound to a relevant raindrop diameter is often too expensive and its cost sometimes overrides its attractiveness. In this study, a physical low-cost method was used to record the DSD of the study area. The Kaplan-Meier method was used to test the aptness of the data to exponential and lognormal distributions, which were subsequently used to formulate the parameterisation of the distributions. This research abrogates the concept of exclusive occurrence of convective storm in tropical regions and presented a new insight into their concurrence appearance.
    MeSH terms: Fresh Water/chemistry*; Malaysia; Models, Chemical*; Rain/chemistry*; Tropical Climate; Kaplan-Meier Estimate
  8. Forde BM, Ben Zakour NL, Stanton-Cook M, Phan MD, Totsika M, Peters KM, et al.
    PLoS One, 2014;9(8):e104400.
    PMID: 25126841 DOI: 10.1371/journal.pone.0104400
    Escherichia coli ST131 is now recognised as a leading contributor to urinary tract and bloodstream infections in both community and clinical settings. Here we present the complete, annotated genome of E. coli EC958, which was isolated from the urine of a patient presenting with a urinary tract infection in the Northwest region of England and represents the most well characterised ST131 strain. Sequencing was carried out using the Pacific Biosciences platform, which provided sufficient depth and read-length to produce a complete genome without the need for other technologies. The discovery of spurious contigs within the assembly that correspond to site-specific inversions in the tail fibre regions of prophages demonstrates the potential for this technology to reveal dynamic evolutionary mechanisms. E. coli EC958 belongs to the major subgroup of ST131 strains that produce the CTX-M-15 extended spectrum β-lactamase, are fluoroquinolone resistant and encode the fimH30 type 1 fimbrial adhesin. This subgroup includes the Indian strain NA114 and the North American strain JJ1886. A comparison of the genomes of EC958, JJ1886 and NA114 revealed that differences in the arrangement of genomic islands, prophages and other repetitive elements in the NA114 genome are not biologically relevant and are due to misassembly. The availability of a high quality uropathogenic E. coli ST131 genome provides a reference for understanding this multidrug resistant pathogen and will facilitate novel functional, comparative and clinical studies of the E. coli ST131 clonal lineage.
    MeSH terms: Anti-Bacterial Agents/pharmacology; Chromosomes, Bacterial; England; Escherichia coli/classification; Escherichia coli/drug effects; Escherichia coli/genetics*; Escherichia coli Infections/microbiology; Humans; Phylogeny; Urinary Tract Infections/microbiology; Genome, Bacterial*; Sequence Analysis, DNA; Computational Biology; Gene Order; Genomics; Drug Resistance, Multiple, Bacterial/genetics; Molecular Sequence Annotation
  9. Shin W, Ismail N
    Cyberpsychol Behav Soc Netw, 2014 Sep;17(9):578-83.
    PMID: 25126969 DOI: 10.1089/cyber.2014.0095
    This study investigated the role of parental and peer mediation in young adolescents' engagement in risk-taking in social networking sites (SNSs). A survey conducted in Malaysia with 469 SNS users aged 13-14 revealed that control-based parental mediation can cause boomerang effects, making young adolescents more inclined to taking risks in SNSs. While discussion-based parental mediation was found to be negatively related to young adolescents' befriending strangers in SNSs, it did not reduce privacy risks. Findings also suggested that peer influence could result in undesirable outcomes. In particular, the more young adolescents talked about Internet-related issues with peers, the more likely they were to disclose personally identifiable information on SNSs.
    MeSH terms: Adolescent; Data Collection; Female; Humans; Male; Parent-Child Relations; Peer Group*; Risk-Taking*; Self Disclosure*; Parenting/psychology*; Internet; Social Networking*; Social Media*
  10. Lau SC, Lim HN, Basri M, Fard Masoumi HR, Ahmad Tajudin A, Huang NM, et al.
    PLoS One, 2014;9(8):e104695.
    PMID: 25127038 DOI: 10.1371/journal.pone.0104695
    In this work, lipase from Candida rugosa was immobilized onto chitosan/graphene oxide beads. This was to provide an enzyme-immobilizing carrier with excellent enzyme immobilization activity for an enzyme group requiring hydrophilicity on the immobilizing carrier. In addition, this work involved a process for the preparation of an enzymatically active product insoluble in a reaction medium consisting of lauric acid and oleyl alcohol as reactants and hexane as a solvent. This product enabled the stability of the enzyme under the working conditions and allowed the enzyme to be readily isolated from the support. In particular, this meant that an enzymatic reaction could be stopped by the simple mechanical separation of the "insoluble" enzyme from the reaction medium. Chitosan was incorporated with graphene oxide because the latter was able to enhance the physical strength of the chitosan beads by its superior mechanical integrity and low thermal conductivity. The X-ray diffraction pattern showed that the graphene oxide was successfully embedded within the structure of the chitosan. Further, the lipase incorporation on the beads was confirmed by a thermo-gravimetric analysis. The lipase immobilization on the beads involved the functionalization with coupling agents, N-hydroxysulfosuccinimide sodium (NHS) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC), and it possessed a high enzyme activity of 64 U. The overall esterification conversion of the prepared product was 78% at 60 °C, and it attained conversions of 98% and 88% with commercially available lipozyme and novozyme, respectively, under similar experimental conditions.
    MeSH terms: Candida/enzymology*; Enzymes, Immobilized/metabolism*; Esterification/physiology; Fatty Alcohols/chemistry; Graphite/chemistry; Hexanes/chemistry; Lauric Acids/chemistry; Lipase/metabolism*; Microscopy, Electron, Scanning; Microspheres; X-Ray Diffraction; Bioreactors*; Chitosan/chemistry; Biocatalysis*; Hydrophobic and Hydrophilic Interactions
  11. Shiraz M, Gani A, Ahmad RW, Adeel Ali Shah S, Karim A, Rahman ZA
    PLoS One, 2014;9(8):e102270.
    PMID: 25127245 DOI: 10.1371/journal.pone.0102270
    The latest developments in mobile computing technology have enabled intensive applications on the modern Smartphones. However, such applications are still constrained by limitations in processing potentials, storage capacity and battery lifetime of the Smart Mobile Devices (SMDs). Therefore, Mobile Cloud Computing (MCC) leverages the application processing services of computational clouds for mitigating resources limitations in SMDs. Currently, a number of computational offloading frameworks are proposed for MCC wherein the intensive components of the application are outsourced to computational clouds. Nevertheless, such frameworks focus on runtime partitioning of the application for computational offloading, which is time consuming and resources intensive. The resource constraint nature of SMDs require lightweight procedures for leveraging computational clouds. Therefore, this paper presents a lightweight framework which focuses on minimizing additional resources utilization in computational offloading for MCC. The framework employs features of centralized monitoring, high availability and on demand access services of computational clouds for computational offloading. As a result, the turnaround time and execution cost of the application are reduced. The framework is evaluated by testing prototype application in the real MCC environment. The lightweight nature of the proposed framework is validated by employing computational offloading for the proposed framework and the latest existing frameworks. Analysis shows that by employing the proposed framework for computational offloading, the size of data transmission is reduced by 91%, energy consumption cost is minimized by 81% and turnaround time of the application is decreased by 83.5% as compared to the existing offloading frameworks. Hence, the proposed framework minimizes additional resources utilization and therefore offers lightweight solution for computational offloading in MCC.
    MeSH terms: Computer Communication Networks*; Computer Storage Devices*; Internet*; Mobile Applications*
  12. Ramlee MH, Kadir MR, Murali MR, Kamarul T
    Med Eng Phys, 2014 Oct;36(10):1322-30.
    PMID: 25127377 DOI: 10.1016/j.medengphy.2014.05.015
    Pilon fractures are commonly caused by high energy trauma and can result in long-term immobilization of patients. The use of an external fixator i.e. the (1) Delta, (2) Mitkovic or (3) Unilateral frame for treating type III pilon fractures is generally recommended by many experts owing to the stability provided by these constructs. This allows this type of fracture to heal quickly whilst permitting early mobilization. However, the stability of one fixator over the other has not been previously demonstrated. This study was conducted to determine the biomechanical stability of these external fixators in type III pilon fractures using finite element modelling. Three-dimensional models of the tibia, fibula, talus, calcaneus, navicular, cuboid, three cuneiforms and five metatarsal bones were reconstructed from previously obtained CT datasets. Bones were assigned with isotropic material properties, while the cartilage was assigned as hyperelastic springs with Mooney-Rivlin properties. Axial loads of 350 N and 70 N were applied at the tibia to simulate the stance and the swing phase of a gait cycle. To prevent rigid body motion, the calcaneus and metatarsals were fixed distally in all degrees of freedom. The results indicate that the model with the Delta frame produced the lowest relative micromovement (0.03 mm) compared to the Mitkovic (0.05 mm) and Unilateral (0.42 mm) fixators during the stance phase. The highest stress concentrations were found at the pin of the Unilateral external fixator (509.2 MPa) compared to the Mitkovic (286.0 MPa) and the Delta (266.7 MPa) frames. In conclusion, the Delta external fixator was found to be the most stable external fixator for treating type III pilon fractures.
    MeSH terms: Biomechanical Phenomena; Humans; Movement; Stress, Mechanical; Tibia/physiology; Tibia/radiography; Tibia/surgery; Tibial Fractures/physiopathology; Tibial Fractures/radiography; Tibial Fractures/surgery*; Tomography, X-Ray Computed; Likelihood Functions; External Fixators*; Finite Element Analysis*; Mechanical Processes*
  13. Mookiah MR, Acharya UR, Koh JE, Chandran V, Chua CK, Tan JH, et al.
    Comput Biol Med, 2014 Oct;53:55-64.
    PMID: 25127409 DOI: 10.1016/j.compbiomed.2014.07.015
    Age-related Macular Degeneration (AMD) is one of the major causes of vision loss and blindness in ageing population. Currently, there is no cure for AMD, however early detection and subsequent treatment may prevent the severe vision loss or slow the progression of the disease. AMD can be classified into two types: dry and wet AMDs. The people with macular degeneration are mostly affected by dry AMD. Early symptoms of AMD are formation of drusen and yellow pigmentation. These lesions are identified by manual inspection of fundus images by the ophthalmologists. It is a time consuming, tiresome process, and hence an automated diagnosis of AMD screening tool can aid clinicians in their diagnosis significantly. This study proposes an automated dry AMD detection system using various entropies (Shannon, Kapur, Renyi and Yager), Higher Order Spectra (HOS) bispectra features, Fractional Dimension (FD), and Gabor wavelet features extracted from greyscale fundus images. The features are ranked using t-test, Kullback-Lieber Divergence (KLD), Chernoff Bound and Bhattacharyya Distance (CBBD), Receiver Operating Characteristics (ROC) curve-based and Wilcoxon ranking methods in order to select optimum features and classified into normal and AMD classes using Naive Bayes (NB), k-Nearest Neighbour (k-NN), Probabilistic Neural Network (PNN), Decision Tree (DT) and Support Vector Machine (SVM) classifiers. The performance of the proposed system is evaluated using private (Kasturba Medical Hospital, Manipal, India), Automated Retinal Image Analysis (ARIA) and STructured Analysis of the Retina (STARE) datasets. The proposed system yielded the highest average classification accuracies of 90.19%, 95.07% and 95% with 42, 54 and 38 optimal ranked features using SVM classifier for private, ARIA and STARE datasets respectively. This automated AMD detection system can be used for mass fundus image screening and aid clinicians by making better use of their expertise on selected images that require further examination.
    MeSH terms: Algorithms; Diagnostic Techniques, Ophthalmological*; Fundus Oculi; Humans; Image Interpretation, Computer-Assisted/methods*; Macular Degeneration/diagnosis*; Models, Statistical; Databases, Factual; Wavelet Analysis
  14. Moniruzzaman M, Rodríguez I, Ramil M, Cela R, Sulaiman SA, Gan SH
    Talanta, 2014 Nov;129:505-15.
    PMID: 25127626 DOI: 10.1016/j.talanta.2014.06.019
    The performance of gas chromatography (GC) combined with a hybrid quadrupole time-of-flight (QTOF) mass spectrometry (MS) system for the determination of volatile and semi-volatile compounds in honey samples is evaluated. After headspace (HS) solid-phase microextraction (SPME) of samples, the accurate mass capabilities of the above system were evaluated for compounds identification. Accurate scan electron impact (EI) MS spectra allowed discriminating compounds displaying the same nominal masses, but having different empirical formulae. Moreover, the use of a mass window with a width of 0.005 Da provided highly specific chromatograms for selected ions, avoiding the contribution of interferences to their peak areas. Additional information derived from positive chemical ionization (PCI) MS spectra and ion product scan MS/MS spectra permitted confirming the identity of novel compounds. The above possibilities are illustrated with examples of honey aroma compounds, belonging to different chemical classes and containing different elements in their molecules. Examples of compounds whose structures could not be described are also provided. Overall, 84 compounds, from a total of 89 species, could be identified in 19 honey samples from 3 different geographic areas in the world. The suitability of responses measured for selected ions, corresponding to above species, for authentication purposes is assessed through principal components analysis.
    MeSH terms: Alkaloids/chemistry; Benzene/chemistry; Carboxylic Acids/chemistry; Esters/chemistry; Food Analysis/methods*; Honey/analysis*; Ions; Gas Chromatography-Mass Spectrometry/methods*; Molecular Weight; Quinolines/chemistry; Terpenes/chemistry; Reproducibility of Results; Principal Component Analysis; Solid Phase Microextraction; Volatile Organic Compounds/analysis*
  15. Moghadamtousi SZ, Kadir HA, Paydar M, Rouhollahi E, Karimian H
    PMID: 25127718 DOI: 10.1186/1472-6882-14-299
    Annona muricata leaves have been reported to have antiproliferative effects against various cancer cell lines. However, the detailed mechanism has yet to be defined. The current study was designed to evaluate the molecular mechanisms of A. muricata leaves ethyl acetate extract (AMEAE) against lung cancer A549 cells.
    MeSH terms: Cell Cycle/drug effects; Cell Survival/drug effects; Humans; Lung Neoplasms/drug therapy; Lung Neoplasms/genetics; Lung Neoplasms/metabolism*; Mitochondria/drug effects; Mitochondria/metabolism*; Plant Extracts/pharmacology*; Signal Transduction/drug effects*; NF-kappa B/metabolism*; Apoptosis/drug effects*; Reactive Oxygen Species/metabolism; Plant Leaves/chemistry; Annona/chemistry*; Cytochromes c/metabolism; Cell Line, Tumor; Cell Proliferation/drug effects; bcl-2-Associated X Protein/metabolism; Membrane Potential, Mitochondrial/drug effects; Caspase 3/metabolism; Caspase 9/metabolism; Cell Cycle Checkpoints
  16. Al-Mekhlafi HM, Anuar TS, Al-Zabedi EM, Al-Maktari MT, Mahdy MA, Ahmed A, et al.
    Parasit Vectors, 2014;7:367.
    PMID: 25127885 DOI: 10.1186/1756-3305-7-367
    Despite the intensive global efforts to control intestinal parasitic infections, the prevalence of soil-transmitted helminth (STH) infections is still very high in many developing countries particularly among children in rural areas.
    MeSH terms: Anthelmintics/administration & dosage; Anthelmintics/therapeutic use; Child; Dose-Response Relationship, Drug; Female; Helminthiasis/drug therapy*; Helminthiasis/epidemiology; Helminthiasis/prevention & control*; Humans; Malaysia/epidemiology; Male; Soil/parasitology*; Vitamin A/administration & dosage; Vitamin A/pharmacology*; Albendazole/administration & dosage; Albendazole/therapeutic use; Dietary Supplements
  17. Ahmed HM, Luddin N, Kannan TP, Mokhtar KI, Ahmad A
    J Endod, 2014 Oct;40(10):1517-23.
    PMID: 25127931 DOI: 10.1016/j.joen.2014.06.013
    The attachment and spreading of mammalian cells on endodontic biomaterials are an area of active research. The purpose of this review is to discuss the cell attachment properties of Portland cement (PC)-based materials by using scanning electron microscope (SEM). In addition, methodological aspects and technical challenges are discussed.
  18. Eksan MS, Noorizan Y, Chew YK, Noorafidah MD, Asma A
    Am J Otolaryngol, 2014 Nov-Dec;35(6):814-5.
    PMID: 25128187 DOI: 10.1016/j.amjoto.2014.07.009
    Embryonal rhabdomyosarcoma of external ear canal is very rare, even in the pediatric population. We report an embryonal rhabdomyosarcoma of external acoustic canal, occurring in a 12-year-old Chinese girl who presented with left ear canal mass. Wide local excision of the mass was done under local anesthesia. Histopathological examination revealed the diagnosis. She then underwent 20 cycles of radiotherapy. CECT scan post treatment showed clearance of tumor cell. Now, patient is disease free for 5 years. We believe that early diagnosis followed by complete resection of the tumor with clear margin and radiotherapy improves the prognosis of the disease.
    MeSH terms: Child; Combined Modality Therapy; Ear Canal*; Ear Neoplasms/diagnosis*; Ear Neoplasms/radiotherapy; Ear Neoplasms/surgery; Female; Humans; Immunohistochemistry; Vimentin/metabolism; Rhabdomyosarcoma, Embryonal/diagnosis*; Rhabdomyosarcoma, Embryonal/radiotherapy; Rhabdomyosarcoma, Embryonal/surgery
  19. Chong CS, Sabir DK, Lorenz A, Bontemps C, Andeer P, Stahl DA, et al.
    Appl Environ Microbiol, 2014 Nov;80(21):6601-10.
    PMID: 25128343 DOI: 10.1128/AEM.01818-14
    Repeated use of the explosive compound hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) on military land has resulted in significant soil and groundwater pollution. Rates of degradation of RDX in the environment are low, and accumulated RDX, which the U.S. Environmental Protection Agency has determined is a possible human carcinogen, is now threatening drinking water supplies. RDX-degrading microorganisms have been isolated from RDX-contaminated land; however, despite the presence of these species in contaminated soils, RDX pollution persists. To further understand this problem, we studied RDX-degrading species belonging to four different genera (Rhodococcus, Microbacterium, Gordonia, and Williamsia) isolated from geographically distinct locations and established that the xplA and xplB (xplAB) genes, which encode a cytochrome P450 and a flavodoxin redox partner, respectively, are nearly identical in all these species. Together, the xplAB system catalyzes the reductive denitration of RDX and subsequent ring cleavage under aerobic and anaerobic conditions. In addition to xplAB, the Rhodococcus species studied here share a 14-kb region flanking xplAB; thus, it appears likely that the RDX-metabolizing ability was transferred as a genomic island within a transposable element. The conservation and transfer of xplAB-flanking genes suggest a role in RDX metabolism. We therefore independently knocked out genes within this cluster in the RDX-degrading species Rhodococcus rhodochrous 11Y. Analysis of the resulting mutants revealed that XplA is essential for RDX degradation and that XplB is not the sole contributor of reducing equivalents to XplA. While XplA expression is induced under nitrogen-limiting conditions and further enhanced by the presence of RDX, MarR is not regulated by RDX.
    MeSH terms: Biotransformation; Multigene Family*; Molecular Sequence Data; Oxidation-Reduction; Triazines/metabolism*; Sequence Analysis, DNA; Actinobacteria/genetics*; Actinobacteria/metabolism*; Explosive Agents/metabolism*; Gene Knockout Techniques
  20. Hassali MA, Wong ZY, Alrasheedy AA, Saleem F, Mohamad Yahaya AH, Aljadhey H
    Health Policy, 2014 Sep;117(3):297-310.
    PMID: 25129135 DOI: 10.1016/j.healthpol.2014.07.014
    This review was conducted to document published literature related to physicians' knowledge, attitudes, and perceptions of generic medicines in low- and middle-income countries (LMICs) and to compare the findings with high-income countries.
    MeSH terms: Attitude; Developing Countries; Humans; Income; Perception; Physicians; Poverty; Drugs, Generic; Developed Countries
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