Browse publications by year: 2017

  1. Grinang J, Tyan PS, Tuen AA, Das I
    Trop Life Sci Res, 2017 Jul;28(2):75-87.
    PMID: 28890762 MyJurnal DOI: 10.21315/tlsr2017.28.2.6
    Data on nutrient contents of freshwater crabs are important for ecological studies and species conservation assessments, especially when the species concerned is threatened among others by habitat destruction and uncontrolled resources utilisation. Indeed comprehensive biological information is required to reconcile the needs between sustainable resources utilisation and conservation of the species. This study documents the nutrient contents of a freshwater crab, Isolapotamon bauense which is listed as 'Vulnerable' in the IUCN Red List of Threatened Species and also being harvested by local community for dietary supplement. Results show that muscles of the freshwater crab contain a substantial amount of nutrients in particular water content (male = 79.31 ± 2.30 %, female = 77.63 ± 0.56 %), protein (male = 77.47 ± 6.11 %, female = 63.28 ± 3.62 %), magnesium (male = 51.48 ± 16.10 mg/g, female = 39.73 ± 6.99 mg/g) and calcium (male = 25.50 ± 6.98 mg/g, female = 39.73 ± 6.99 mg/g). Means of nutrient contents between male and female crabs are not significantly different. It is estimated that an individual of I. bauense with weight range of 56-139 g contained on average of 0.35 ± 0.15 g of protein. Our estimation also shows that the number of individuals of the freshwater crab required to meet the recommended daily protein intakes of the community concerned is in the range 35-96 individuals for children, 130-188 individuals for adolescents, 171-179 individuals for men and 149-159 individuals for women. The results imply that harvesting of wild I. bauense as a source of protein supplement naturally may not be practical because of its relatively low population abundance, and conservation of the species for its ecological roles may thus be preferred.
    MeSH terms: Adolescent; Animals; Calcium, Dietary; Child; Brachyura; Female; Fresh Water; Humans; Magnesium; Male; Water; Ecosystem; Dietary Supplements; Endangered Species; Recommended Dietary Allowances
  2. Ab Hamid S, Md Rawi CS
    Trop Life Sci Res, 2017 Jul;28(2):89-105.
    PMID: 28890763 MyJurnal DOI: 10.21315/tlsr2017.28.2.7
    Leaf litter decomposition in a tropical stream was examined in two types of leaf packs; single species leaf packs of Pometia pinnata and two species leaf packs of equal combination of Pometia pinnata and Dolichandrone spathacea leaves. Both leaf packs were immersed in a river and weekly examined for remains of decomposed leaves and presence of EPT. In the control leaf packs, leaves in the two species leaf packs treatments decomposed within 35 days, faster than in single species leaf packs which decomposed after 42 days. In the presence of EPT, the leaf breakdown took 28 days in two species and 35 days for single species leaf packs. Higher abundance of EPT was observed in single species leaf packs but its diversity was higher in two species leaf packs. Litter breakdown in the stream was faster in the presence of EPT and softer leaves of D. spathacea with higher nitrogen content underwent faster decomposition and sustained higher numbers of EPT.
    MeSH terms: Nitrogen; Trees; Plant Leaves; Bignoniaceae; Rivers
  3. Azhar NS, Md Zin NH, Hamid THTA
    Trop Life Sci Res, 2017 Jul;28(2):107-118.
    PMID: 28890764 MyJurnal DOI: 10.21315/tlsr2017.28.2.8
    In this study, a Lactic acid bacteria (LAB) strain was isolated on MRS medium from gastro-intestinal tissues of Broadhead catfish (Clarias macrocephalus). Out of 50 isolates, 25 isolates were found to be positive on lactose utilisation test and were identified to be gram positive cocci. Using disc diffusion methods, one out of 22 isolates, i.e., a strain A5 demonstrated inhibitions against three indicator organisms; Bacillus cereus, Staphylococcus aureus and Salmonella thyphimurium. Partial 16S rDNA sequencing identified isolate A5 as a member of Lactococcus lactis, with 100% DNA homology. Cell free supernatant fluid from Lactococcus lactis A5 showed inhibitory activities against both gram positive pathogens (Bacillus cereus and Staphylococcus aureus) and gram negative pathogens (Salmonella thyphimurium). Chloroform precipitated bacteriocin retained antagonistic activities in the presence of catalase and lysozyme; and was completely inactivated by Proteinase K treatment. The bacteriocin has a molecular weight of 3.4 kDa, based on SDS-PAGE analysis and the extract was heat stable at 37°C and 65°C, for 15 minutes. The antibacterial activity was suppressed with the addition of EDTA but was significantly increased with the addition of SDS, Triton X-100, Tween 20 and Tween 80. This bacteriocin belongs to class 1 bacteriocin, which was shown to have a nisin-like properties. This strain can be used as potential probiotics in animal or aquaculture feeding; and the bacteriocin it produces will be useful in food preservative.
    MeSH terms: Animals; Bacillus cereus; Catalase; Chloroform; Edetic Acid; Food Preservatives; Gram-Positive Cocci; Lactose; Muramidase; Nisin; Polysorbates; Staphylococcus aureus; Lactococcus lactis; Octoxynol; Endopeptidase K
  4. Fadzly N, Adeeb S, Md Sah ASR
    Trop Life Sci Res, 2017 Jul;28(2):127-141.
    PMID: 28890766 MyJurnal DOI: 10.21315/tlsr2017.28.2.10
    In this paper, we studied some biological aspects of big eye scad (Selar crumenophthalmus) from Maldives. The fish sample was collected from the local fish market at Bangaa Faru, Male, Maldives. The length of the samples were ranged from 7.7 cm to 24.5 cm (mean value = 16.85 ± 2.82 cm) in fork length. Body weight ranged between 8 g to 255.6 g (mean value = 87.76 ± 40.41 g). The exponent values (b slope) of lengthweight relationship of S. crumenophthalmus are 2.9838 for females and 2.7687 for males; indicating negative allometric growth pattern for both sexes. Synchronous reproductive behaviour was observed in both sexes and a pronounced peak of Gonadosomatic index was observed in females in January 2013. It is estimated that length at first maturity (L50) for females is at 19.39 cm FL and for males at 21.76 cm FL. Our result also suggest that big eye scad have a reduced swimming capability, resulting the species to be easily caught. Careful planning and management should be implemented to prevent the big eye scad from being overfished.
    MeSH terms: Animals; Body Weight; Female; Fishes; Indian Ocean Islands; Male; Perciformes; Swimming; Reproductive Behavior
  5. Ab Hamid S, Md Rawi CS
    Trop Life Sci Res, 2017 Jul;28(2):143-162.
    PMID: 28890767 MyJurnal DOI: 10.21315/tlsr2017.28.2.11
    The Ephemeroptera, Plecoptera and Trichoptera (EPT) community structure and the specific sensitivity of certain EPT genera were found to be influenced by water parameters in the rivers of Gunung Jerai Forest Reserve (GJFR) in the north of peninsular Malaysia. The scores of EPT taxa richness of >10 in all rivers indicated all rivers' habitats were non-impacted, having good water quality coinciding with Class I and Class II of Malaysian water quality index (WQI) classification of potable water. The abundance of EPT was very high in Teroi River (9,661 individuals) but diversity was lower (22 genera) than Tupah River which was highly diverse (28 genera) but lower in abundance (4,263 individuals). The lowest abundance and moderate diversity was recorded from Batu Hampar River (25 genera). Baetis spp. and Thalerosphyrus spp., Neoperla spp. and Cheumatopsyche spp. were the most common genera found. Classification for all rivers using EPT taxa Richness Index and WQI gave different category of water quality, respectively. The WQI classified Tupah and Batu Hampar rivers into Class II and Teroi River (Class I) was two classes above the classification of the EPT taxa Richness Index.
    MeSH terms: Animals; Malaysia; Ecosystem; Rivers; Water Quality; Drinking Water; Ephemeroptera; Forests
  6. Eshak MB, Omar WMW
    Trop Life Sci Res, 2017 Jul;28(2):163-177.
    PMID: 28890768 MyJurnal DOI: 10.21315/tlsr2017.28.2.12
    The importance of polyunsaturated fatty acid (PUFA) in microalgae was widely reported. In this study, six isolated microalgae from Teluk Aling, Penang National Park were screened for PUFA contents. Isochrysis maritima showed the best polyunsaturated fatty acids essential for aquaculture species compared to other microalgal species tested. This species is a good choice as aquaculture feed due to its small size (3-7 μm), which is appropriate size for ingestion. The maximum specific growth rate of this species was also high (0.52-0.82 days(-1)) and comparable with many recognised aquaculture microalgae. On the other hand, this species was also able to be cultivated successfully in big volume (1000 L culture medium) with open hatchery condition, which will optimise the production cost. Low ratio of omega-6 to omega-3 essential fatty acids (EFA) recorded in I. maritima at any growth phases (0.32-0.45) also indicate optimal values for feeding.
    MeSH terms: Parks, Recreational; Culture Media; Eating; Fatty Acids, Essential; Fatty Acids, Unsaturated; Fatty Acids, Omega-3; Aquaculture; Microalgae; Haptophyta
  7. Rosli NRM, Yahya K
    Trop Life Sci Res, 2017 Jul;28(2):189-199.
    PMID: 28890770 MyJurnal DOI: 10.21315/tlsr2017.28.2.14
    The study of river water quality plays an important role in assessing the pollution status and health of the water bodies. Human-induced activities such as domestic activities, aquaculture, agriculture and industries have detrimentally affected the river water quality. Pinang River is one of the important rivers in Balik Pulau District that supplies freshwater for human consumption. A total of 442 physical and chemical parameters data of the Pinang River, Balik Pulau catchment were analysed to determine the sources of pollutants entering the river. Non-supervised artificial neural network (ANN) was employed to classify and cluster the river into upstream, middle-stream and downstream zones. The monitored data and non-supervised ANN analysis demonstrated that the source of nitrate was derived from the upper part of the Pinang River, Balik Pulau while the sources of nitrite, ammonia and ortho-phosphate are predominant at the middle-stream of the river system. Meanwhile, the sources of high total suspended solid and biological oxygen demand were concentrated at the downstream of the river.
    MeSH terms: Agriculture; Ammonia; Fresh Water; Nitrates; Nitrites; Phosphates; Aquaculture; Rivers; Biological Oxygen Demand Analysis; Water Quality
  8. Jamil N, Ismail WNW, Abidin SS, Amaran MA, Hazali R
    Trop Life Sci Res, 2017 Jul;28(2):201-213.
    PMID: 28890771 MyJurnal DOI: 10.21315/tlsr2017.28.2.15
    A survey on termite species composition was conducted in Samunsam Wildlife Sanctuary, Sarawak in February 2015. Overall 19 species of termite belonging to 13 genera and 8 subfamilies was found in the sanctuary. It was recorded the subfamily of Termitinae had the highest number of species (6 species, equal to 31.58% of total species), followed by Nasutermitinae (3 species, 15.79%), Macrotermitinae, Amitermitinae, Rhinotermitinae, Coptotermitinae, (2 species, 10.53% respectively), and Heterotermitinae, Termitogetoninae (1 species, 5.26% respectively). Since this rapid survey is the first termite assemblage representation in Samunsam Wildlife Sanctuary, the preliminary result may serve as the baseline data for termite composition in the area. Therefore, a whole coverage for the area within this sanctuary would definitely increase the number of termite species found in the sanctuary.
    MeSH terms: Animals; Animals, Wild; Malaysia; Surveys and Questionnaires; Ecosystem; Isoptera
  9. Shen CL, Klein A, Chin KY, Mo H, Tsai P, Yang RS, et al.
    Ann N Y Acad Sci, 2017 Aug;1401(1):150-165.
    PMID: 28891093 DOI: 10.1111/nyas.13449
    Osteoporosis, a degenerative bone disease, is characterized by low bone mass and microstructural deterioration of bone tissue resulting in aggravated bone fragility and susceptibility to fractures. The trend of extended life expectancy is accompanied by a rise in the prevalence of osteoporosis and concomitant complications in the elderly population. Epidemiological evidence has shown an association between vitamin E consumption and the prevention of age-related bone loss in elderly women and men. Animal studies show that ingestion of vitamin E, especially tocotrienols, may benefit bone health in terms of maintaining higher bone mineral density and improving bone microstructure and quality. The beneficial effects of tocotrienols on bone health appear to be mediated via antioxidant/anti-inflammatory pathways and/or 3-hydroxy-3-methylglutaryl coenzyme A mechanisms. We discuss (1) an overview of the prevalence and etiology of osteoporosis, (2) types of vitamin E (tocopherols versus tocotrienols), (3) findings of tocotrienols and bone health from published in vitro and animal studies, (4) possible mechanisms involved in bone protection, and (5) challenges and future direction for research.
    MeSH terms: Animals; Bone and Bones/drug effects; Bone and Bones/pathology; Bone and Bones/physiology; Humans; Osteoporosis/metabolism; Osteoporosis/pathology; Osteoporosis/prevention & control*; Bone Density/drug effects*; Bone Density/physiology; Tocotrienols/pharmacology; Tocotrienols/therapeutic use*; Translational Medical Research/methods*; Translational Medical Research/trends
  10. Thomson PJ, Goodson ML, Smith DR
    J Oral Pathol Med, 2017 Nov;46(10):902-910.
    PMID: 28891106 DOI: 10.1111/jop.12641
    BACKGROUND: Contemporary potentially malignant disorder management is based upon provisional histological diagnosis followed by interventional surgery to excise or ablate 'high-risk' mucosal lesions. Although the majority of patients achieve disease-free status post-treatment, others develop further or persistent disease unresponsive to intervention.

    METHODS: A detailed, retrospective clinico-pathological review of treatment resistant potentially malignant lesions, from a 590 patient cohort treated by CO2 laser surgery and followed for a mean of 7.3 years, was undertaken. Clinical outcome was determined at study census date (31 December 2014).

    RESULTS: A total of 87 patients (15%) exhibited PMD disease resistant to treatment: 34 (6%) became disease free following further treatment, whilst 53 (9%) had persistent disease despite intervention. Disease-free patients were younger, changed lesion appearance from erythroleukoplakia to leukoplakia (P = .004), developed further lesions at new sites, demonstrated reduction in dysplasia severity with time and required multiple treatments to achieve disease-free status (P = .0005). In contrast, persistent disease patients were older, male, often presented with proliferative verrucous leukoplakia (PVL) on gingival and alveolar sites, displayed less severe dysplasia initially and underwent laser ablation rather than excision (P = .027).

    CONCLUSION: Despite clinico-pathological profiling of treatment resistant patients, the precise inter-relationship between the inherent nature of potentially malignant disease and the external influence of treatment intervention remains obscure.

    MeSH terms: Adult; Aged; Aged, 80 and over; Female; Humans; Male; Middle Aged; Mouth Neoplasms/pathology*; Mouth Neoplasms/therapy; Precancerous Conditions/pathology*; Precancerous Conditions/therapy; Retrospective Studies; Treatment Failure
  11. Péré-Védrenne C, Flahou B, Loke MF, Ménard A, Vadivelu J
    Helicobacter, 2017 Sep;22 Suppl 1.
    PMID: 28891140 DOI: 10.1111/hel.12407
    The current article is a review of the most important and relevant literature published in 2016 and early 2017 on non-Helicobacter pylori Helicobacter infections in humans and animals, as well as interactions between H. pylori and the microbiota of the stomach and other organs. Some putative new Helicobacter species were identified in sea otters, wild boars, dogs, and mice. Many cases of Helicobacter fennelliae and Helicobacter cinaedi infection have been reported in humans, mostly in immunocompromised patients. Mouse models have been used frequently as a model to investigate human Helicobacter infection, although some studies have investigated the pathogenesis of Helicobacters in their natural host, as was the case for Helicobacter suis infection in pigs. Our understanding of both the gastric and gut microbiome has made progress and, in addition, interactions between H. pylori and the microbiome were demonstrated to go beyond the stomach. Some new approaches of preventing Helicobacter infection or its related pathologies were investigated and, in this respect, the probiotic properties of Saccharomyces, Lactobacillus and Bifidobacterium spp. were confirmed.
    MeSH terms: Gastrointestinal Microbiome*; Animals; Disease Models, Animal; Humans; Helicobacter Infections/microbiology*; Helicobacter Infections/veterinary*; Helicobacter/classification*; Helicobacter/isolation & purification*; Probiotics/administration & dosage; Microbial Interactions
  12. Abd Murad NB, Mohamed Nor NMI, Shohaimi S, Mohd Zainudin NAI
    J Appl Microbiol, 2017 Dec;123(6):1533-1546.
    PMID: 28891270 DOI: 10.1111/jam.13582
    AIMS: The aims of this study were to identify the Fusarium isolates based on translation elongation factor (tef) 1α sequence, to determine the genetic diversity among isolates and species using selected microsatellite markers and to examine the pathogenicity of Fusarium isolates causing fruit rot disease of banana.

    METHODS AND RESULTS: One-hundred and thirteen microfungi isolates were obtained from fruit rot infected banana in Peninsular Malaysia. However, this study was focused on the dominant number of the discovered microfungi that belongs to the genus Fusarium; 48 isolates of the microfungi have been identified belonging to 11 species of Fusarium, namely Fusarium incarnatum, Fusarium equiseti, Fusarium camptoceras, Fusarium solani, Fusarium concolor, Fusarium oxysporum, Fusarium proliferatum, Fusarium verticillioides, Fusarium sacchari, Fusarium concentricum and Fusarium fujikuroi. All Fusarium isolates were grouped into their respective clades indicating their similarities and differences in genetic diversity among isolates. Out of 48 Fusarium isolates tested, 42 isolates caused the fruit rot symptom at different levels of severity based on Disease Severity Index (DSI). The most virulent isolate was F. proliferatum B2433B with DSI of 100%.

    CONCLUSIONS: All the isolated Fusarium species were successfully identified and some of them were confirmed as the causal agents of pre- and postharvest fruit rot in banana across Peninsular Malaysia.

    SIGNIFICANCE AND IMPACT OF THE STUDY: Our results will provide additional information regarding new report of Fusarium species in causing banana fruit rot and in the search of potential biocontrol agent of the disease.

    MeSH terms: Fusarium/genetics*; Fusarium/isolation & purification; Fusarium/pathogenicity; Malaysia; Phylogeny; Genetic Variation; Peptide Elongation Factor 1/chemistry; Musa/microbiology*
  13. Zainul NH, Ma ZF, Besari A, Siti Asma H, Rahman RA, Collins DA, et al.
    Epidemiol Infect, 2017 Oct;145(14):3012-3019.
    PMID: 28891459 DOI: 10.1017/S0950268817002011
    Little is known about Clostridium difficile infection (CDI) in Asia. The aims of our study were to explore (i) the prevalence, risk factors and molecular epidemiology of CDI and colonization in a tertiary academic hospital in North-Eastern Peninsular Malaysia; (ii) the rate of carriage of C. difficile among the elderly in the region; (iii) the awareness level of this infection among the hospital staffs and students. For stool samples collected from hospital inpatients with diarrhea (n = 76) and healthy community members (n = 138), C. difficile antigen and toxins were tested by enzyme immunoassay. Stool samples were subsequently analyzed by culture and molecular detection of toxin genes, and PCR ribotyping of isolates. To examine awareness among hospital staff and students, participants were asked to complete a self-administered questionnaire. For the hospital and community studies, the prevalence of non-toxigenic C. difficile colonization was 16% and 2%, respectively. The prevalence of CDI among hospital inpatients with diarrhea was 13%. Out of 22 C. difficile strains from hospital inpatients, the toxigenic ribotypes 043 and 017 were most common (both 14%). In univariate analysis, C. difficile colonization in hospital inpatients was significantly associated with greater duration of hospitalization and use of penicillin (both P < 0·05). Absence of these factors was a possible reason for low colonization in the community. Only 3% of 154 respondents answered all questions correctly in the awareness survey. C. difficile colonization is prevalent in a Malaysian hospital setting but not in the elderly community with little or no contact with hospitals. Awareness of CDI is alarmingly poor.
    MeSH terms: Adult; Aged; Aged, 80 and over; Carrier State/microbiology; Carrier State/epidemiology*; Clostridium Infections/complications; Clostridium Infections/microbiology; Clostridium Infections/epidemiology*; Cross Infection/microbiology; Cross Infection/epidemiology*; Diarrhea/etiology; Diarrhea/microbiology; Diarrhea/epidemiology; Feces/microbiology; Female; Humans; Health Knowledge, Attitudes, Practice; Malaysia/epidemiology; Male; Middle Aged; Risk Factors; Prevalence; Clostridium difficile/isolation & purification*; Molecular Epidemiology*; Ribotyping; Young Adult; Tertiary Care Centers
  14. Zafar R, Kamel N, Naufal M, Malik AS, Dass SC, Ahmad RF, et al.
    J Integr Neurosci, 2017;16(3):275-289.
    PMID: 28891512 DOI: 10.3233/JIN-170016
    Decoding of human brain activity has always been a primary goal in neuroscience especially with functional magnetic resonance imaging (fMRI) data. In recent years, Convolutional neural network (CNN) has become a popular method for the extraction of features due to its higher accuracy, however it needs a lot of computation and training data. In this study, an algorithm is developed using Multivariate pattern analysis (MVPA) and modified CNN to decode the behavior of brain for different images with limited data set. Selection of significant features is an important part of fMRI data analysis, since it reduces the computational burden and improves the prediction performance; significant features are selected using t-test. MVPA uses machine learning algorithms to classify different brain states and helps in prediction during the task. General linear model (GLM) is used to find the unknown parameters of every individual voxel and the classification is done using multi-class support vector machine (SVM). MVPA-CNN based proposed algorithm is compared with region of interest (ROI) based method and MVPA based estimated values. The proposed method showed better overall accuracy (68.6%) compared to ROI (61.88%) and estimation values (64.17%).
    MeSH terms: Algorithms; Brain; Goals; Humans; Magnetic Resonance Imaging; Multivariate Analysis; Linear Models; Support Vector Machine
  15. Cacha LA, Ali J, Rizvi ZH, Yupapin PP, Poznanski RR
    J Integr Neurosci, 2017;16(4):493-509.
    PMID: 28891529 DOI: 10.3233/JIN-170038
    Using steady-state electrical properties of non-ohmic dendrite based on cable theory, we derive electrotonic potentials that do not change over time and are localized in space. We hypothesize that clusters of such stationary, local and permanent pulses are the electrical signatures of enduring memories which are imprinted through nonsynaptic plasticity, encoded through epigenetic mechanisms, and decoded through electrotonic processing. We further hypothesize how retrieval of an engram is made possible by integration of these permanently imprinted standing pulses in a neural circuit through neurotransmission in the extracellular space as part of conscious recall that acts as a guiding template in the reconsolidation of long-term memories through novelty characterized by uncertainty that arises when new fragments of memories reinstate an engram by way of nonsynaptic plasticity that permits its destabilization. Collectively, these findings seem to reinforce this hypothesis that electrotonic processing in non-ohmic dendrites yield insights into permanent electrical signatures that could reflect upon enduring memories as fragments of long-term memory engrams.
    MeSH terms: Dendrites; Extracellular Space; Memory; Synaptic Transmission; Mental Recall; Uncertainty; Epigenesis, Genetic; Memory, Long-Term
  16. Beevi Z, Low WY, Hassan J
    Am J Clin Hypn, 2017 Oct;60(2):172-191.
    PMID: 28891771 DOI: 10.1080/00029157.2017.1280659
    Hypnosis has been shown to help pregnant women experience improved labor and postpartum periods. The present study compares the differences between experimental (n = 23) and control groups (n = 22) on specific variables measured both during labor and 24 hr postpartum. The participants in the experimental group received the hypnosis intervention at weeks 16, 20, 28, and 36 of pregnancy, while those in the control group received only routine antenatal care. The data collected at the labor stage describe the length of the labor stage, pain relief used during labor, the method of delivery, and the type of assisted vaginal delivery. Within 24 hr of delivery, data on neonatal birth weight, neonatal Apgar scores, and self-reported pain were obtained. The labor stage results showed no significant differences in the length of the second and third stages of labor. Although the participants in the experimental group reported higher pain levels immediately prior to, during, and immediately after delivery, their use of pethidine during labor was significantly lower than the control group participants. None of the experimental group participants opted for an epidural, and they had a greater number of assisted vaginal deliveries than the control group participants. The 24 hr postpartum results showed that the neonates of the experimental group participants had nonsignificantly higher Apgar scores than those of the women in the control group. Group differences in neonatal weight were not significant. The results of the present study indicate that hypnosis is useful for assisting pregnant women during labor and the postpartum period.
    MeSH terms: Adult; Apgar Score*; Female; Humans; Hypnosis/methods*; Infant, Newborn; Labor, Obstetric/physiology*; Obstetric Labor Complications/prevention & control*; Pregnancy; Young Adult
  17. Ismail NAH, Wee SY, Aris AZ
    Chemosphere, 2017 Dec;188:375-388.
    PMID: 28892772 DOI: 10.1016/j.chemosphere.2017.08.150
    Fishes are a major protein food source for humans, with a high economic value in the aquaculture industry. Because endocrine disrupting compounds (EDCs) have been introduced into aquatic ecosystems, the exposure of humans and animals that depend on aquatic foods, especially fishes, should be seriously considered. EDCs are emerging pollutants causing global concern because they can disrupt the endocrine system in aquatic organisms, mammals, and humans. These pollutants have been released into the environment through many sources, e.g., wastewater treatment plants, terrestrial run-off (industrial activities, pharmaceuticals, and household waste), and precipitation. The use of pharmaceuticals, pesticides, and fertilizers for maintaining and increasing fish health and growth also contributes to EDC pollution in the water body. Human and animal exposure to EDCs occurs via ingestion of contaminated matrices, especially aquatic foodstuffs. This paper aims to review human EDC exposure via fish consumption. In respect to the trace concentration of EDCs in fish, types of instrument and clean-up method are of great concerns.
    MeSH terms: Animals; Endocrine System/drug effects*; Endocrine System/metabolism; Fishes/growth & development; Fishes/metabolism*; Humans; Water Pollutants, Chemical/analysis; Water Pollutants, Chemical/toxicity*; Ecosystem; Aquaculture/methods*; Risk Assessment; Food Chain; Endocrine Disruptors/analysis; Endocrine Disruptors/toxicity*; Biota/drug effects*
  18. Ismail N, Ismail M, Azmi NH, Bakar MFA, Yida Z, Abdullah MA, et al.
    Biomed Pharmacother, 2017 Nov;95:780-788.
    PMID: 28892789 DOI: 10.1016/j.biopha.2017.08.074
    Though the causes of Alzheimer's disease (AD) are yet to be understood, much evidence has suggested that excessive amyloid-β (Aβ) accumulation due to abnormal amyloid-β precursor protein (APP) processing and Aβ metabolism are crucial processes towards AD pathogenesis. Hence, approaches aiming at APP processing and Aβ metabolism are currently being actively pursued for the management of AD. Studies suggest that high cholesterol and a high fat diet have harmful effects on cognitive function and may instigate the commencement of AD pathogenesis. Despite the neuropharmacological attributes of black cumin seed (Nigella sativa) extracts and its main active compound, thymoquinone (TQ), limited records are available in relation to AD research. Nanoemulsion (NE) is exploited as drug delivery systems due to their capacity of solubilising non-polar active compounds and is widely examined for brain targeting. Herewith, the effects of thymoquinone-rich fraction nanoemulsion (TQRFNE), thymoquinone nanoemulsion (TQNE) and their counterparts' conventional emulsion in response to high fat/cholesterol diet (HFCD)-induced rats were investigated. Particularly, the Aβ generation; APP processing, β-secretase 1 (BACE1), γ-secretases of presenilin 1 (PSEN1) and presenilin 2 (PSEN2), Aβ degradation; insulin degrading enzyme (IDE), Aβ transportation; low density lipoprotein receptor-related protein 1 (LRP1) and receptor for advanced glycation end products (RAGE) were measured in brain tissues. TQRFNE reduced the brain Aβ fragment length 1-40 and 1-42 (Aβ40 and Aβ42) levels, which would attenuate the AD pathogenesis. This reduction could be due to the modulation of β- and γ-secretase enzyme activity, and the Aβ degradation and transportation in/out of the brain. The findings show the mechanistic actions of TQRFNE in response to high fat and high cholesterol diet associated to Aβ generation, degradation and transportation in the rat's brain tissue.
    MeSH terms: Advanced Glycosylation End Product-Specific Receptor/metabolism*; Animals; Emulsions/chemistry; Gene Expression Regulation/drug effects; Hippocampus/drug effects; Hippocampus/metabolism; Insulysin/metabolism*; Male; Protein Processing, Post-Translational/drug effects; RNA, Messenger/genetics; RNA, Messenger/metabolism; Down-Regulation/drug effects; Benzoquinones/pharmacology*; Aspartic Acid Endopeptidases/metabolism*; Amyloid beta-Protein Precursor/metabolism*; Rats, Sprague-Dawley; Low Density Lipoprotein Receptor-Related Protein-1/metabolism*; Nanoparticles/chemistry*; Presenilin-2/metabolism; Amyloid Precursor Protein Secretases/metabolism*; Diet, High-Fat
  19. Hasan SS, Kow CS, Thiruchelvam K, Chong DWK, Ahmed SI
    Neuroepidemiology, 2017;49(1-2):82-90.
    PMID: 28892805 DOI: 10.1159/000480433
    BACKGROUND/AIMS: Old age and institutionalization in care homes are associated with increased use of risk medications affecting the central nervous system (CNS). This study evaluated medication utilization and appropriateness; and assessed frailty among residents of Malaysian aged care homes.

    METHODS: The subjects of this study included 202 elderly (≥65 years) residents of 17 aged care homes in suburban peninsular Malaysia. Frailty was measured using the Groningen Frailty Indicator (GFI) score and independence in daily living was measured as KATZ activity of daily living score. Medication appropriateness was assessed using the Medication Appropriateness Index (MAI) and 2015 Beers' criteria for Potentially Inappropriate Medication (PIM).

    RESULTS: CNS medications constituted about 16% of the total, with an average of 0.8 ± 1.1 medications per resident, which reduced to 0.5 ± 0.8 medications after 3 months. Frailty (154/202) and polypharmacy (90/202) were highly prevalent in study subjects. Subjects on CNS medications had significantly higher GFI score (7.1 vs. 5.9; p = 0.031), polypharmacy (57.8 vs. 35.3%; p = 0.002), number of PIMs (0.9 vs. 0.2; p = 0.001), and mean summed MAI score (3.6 vs. 2.6; p = 0.015) than subjects not on CNS medications. Medication number was also significantly correlated with GFI (r = 0.194; p = 0.006) and KATZ (r = 0.141; p = 0.046) scores.

    CONCLUSION: Frailty and polypharmacy were highly prevalent among aged care home subjects taking CNS medications. These findings support the notion that periodic regular medication review should improve the overall use of medications in elderly patients.

  20. Luo N, Teng TK, Tay WT, Anand IS, Kraus WE, Liew HB, et al.
    Am Heart J, 2017 Sep;191:75-81.
    PMID: 28888273 DOI: 10.1016/j.ahj.2017.06.016
    BACKGROUND: Assessing health-related quality of life (HRQoL) in patients with heart failure (HF) is an important goal of clinical care and HF research. We sought to investigate ethnic differences in perceived HRQoL and its association with mortality among patients with HF and left ventricular ejection fraction ≤35%, controlling for demographic characteristics and HF severity.

    METHODS AND RESULTS: We compared 5697 chronic HF patients of Indian (26%), white (23%), Chinese (17%), Japanese/Koreans (12%), black (12%), and Malay (10%) ethnicities from the HF-ACTION and ASIAN-HF multinational studies using the Kansas City Cardiomyopathy Questionnaire (KCCQ; range 0-100; higher scores reflect better health status). KCCQ scores were lowest in Malay (58±22) and Chinese (60±23), intermediate in black (64±21) and Indian (65±23), and highest in white (67±20) and Japanese or Korean patients (67±22) after adjusting for age, sex, educational status, HF severity, and risk factors. Self-efficacy, which measures confidence in the ability to manage symptoms, was lower in all Asian ethnicities (especially Japanese/Koreans [60±26], Malay [66±23], and Chinese [64±28]) compared to black (80±21) and white (82±19) patients, even after multivariable adjustment (P

    MeSH terms: Aged; Canada/epidemiology; Ethnic Groups*; Female; France/epidemiology; Health Status*; Heart Failure/ethnology*; Heart Failure/physiopathology; Heart Failure/psychology; Humans; Male; Middle Aged; Prognosis; Prospective Studies; Quality of Life*; Risk Factors; United States/epidemiology; Incidence; Survival Rate/trends; Ventricular Function, Left/physiology; Risk Assessment*
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