Browse publications by year: 2017

  1. Liu K, Fadzly N, Mansor A, Zakaria R, Ruppert N, Lee CY
    Plant Signal Behav, 2017 Oct 03;12(10):e1371890.
    PMID: 28841358 DOI: 10.1080/15592324.2017.1371890
    Amorphophallus bufo is a rarely studied plant in Malaysian tropical rainforests. We measured the spectral reflectance of different developmental stages of A. bufo (seedlings, juveniles and adults), background soil/ debris and leaves from other neighboring plant species. Results show that the leaves of A. bufo seedling have a similar reflectance curve as the background soil and debris. Adults and juveniles of A. bufo are similar to other neighboring plants' leaf colors. We hypothesize that the cryptic coloration of A. bufo seedlings plays an important role in camouflage and that the numerous black spots on the surface of the petioles and rachises, may serve as a defensive mimicry against herbivores.
    MeSH terms: Animals; Insects/pathogenicity; Plant Leaves/metabolism*; Plant Leaves/parasitology*; Amorphophallus/metabolism*; Amorphophallus/parasitology*; Seedlings/metabolism; Seedlings/parasitology; Herbivory/physiology
  2. Abdul Rashid AM, Md Noh MSF
    BMC Neurol, 2017 Aug 25;17(1):165.
    PMID: 28841841 DOI: 10.1186/s12883-017-0944-9
    BACKGROUND: Non-traumatic, spontaneous subarachnoid hemorrhage occurs in approximately 85% of cases where there is a ruptured saccular aneurysm. An additional 10% of cases arise from non-aneurysmal peri-mesencephalic hemorrhages.

    CASE PRESENTATION: We report a rare case of a young female, with underlying Evans syndrome, who was initially thought to have non-hemorrhagic stroke, eventually diagnosed having isolated non-traumatic, non-aneurysmal convexal subarachnoid haemorrhage.

    CONCLUSIONS: Spontaneous non-traumatic, non-aneurysmal convexal subarachnoid hemorrhage is a rare entity - of which there are multiple possible etiologies.

    MeSH terms: Adult; Anemia, Hemolytic, Autoimmune*; Female; Humans; Subarachnoid Hemorrhage*; Thrombocytopenia*
  3. Khai Luen K, Wan Sulaiman WA
    J Foot Ankle Surg, 2017 8 27;56(5):1114-1120.
    PMID: 28842095 DOI: 10.1053/j.jfas.2017.04.024
    Sensate, durable heel pad reconstruction is challenging. The present study assessed the functional outcomes after heel pad reconstruction using various flap techniques at our institution. From June 2011 to June 2016 (5-year period), 7 consecutive patients underwent heel pad reconstruction for various etiologies, with 3 microvascular free flaps (42.9%; 2 musculocutaneous flaps [66.7%] and 1 contralateral medial plantar flap [33.3%]) and 4 local pedicle flaps (57.1%; 3 instep medial plantar artery flaps [75.0%] and 1 distally based reverse sural flap [25.0%]). The patient records and demographic data were reviewed, and surgically related information was obtained and analyzed. The subjective components of the American Orthopaedic Foot and Ankle Society hindfoot clinical ratings scale were used to evaluate the pain and functional outcomes. Sensation was assessed using Semmes-Weinstein monofilaments, and ulcer recurrence was recorded. The mean age of the patients was 41.7 (range 11 to 70) years, the mean defect size was 59 (range 12 to 270) cm2, and the mean follow-up duration was 22 (range 15 to 43) months. Complete flap survival was achieved without significant complications in all 7 patients. Patients treated with the sensate medial plantar artery flap recorded the highest mean American Orthopaedic Foot and Ankle Society score of 57.3 (maximum score of 60) and experienced a return of deep sensation at 6 (range 6 to 24) months and protective sensation at 1 year. This was followed by the reverse sural flap and the musculocutaneous flap. No recurrent heel ulceration was observed in our series of patients. In conclusion, the sensate medial plantar flap is a satisfactory method for coverage of small- to moderate-size heel defects.
    MeSH terms: Adolescent; Aged; Child; Female; Follow-Up Studies; Graft Survival; Heel/physiopathology; Heel/surgery*; Humans; Malaysia; Male; Middle Aged; Retrospective Studies; Surgical Flaps/blood supply; Surgical Flaps/transplantation*; Wound Healing/physiology; Cohort Studies; Soft Tissue Injuries/surgery; Risk Assessment; Reconstructive Surgical Procedures/methods*; Young Adult
  4. Javed E, Faye I, Malik AS, Abdullah JM
    J Neurosci Methods, 2017 11 01;291:150-165.
    PMID: 28842191 DOI: 10.1016/j.jneumeth.2017.08.020
    BACKGROUND: Simultaneous electroencephalography (EEG) and functional magnetic resonance image (fMRI) acquisitions provide better insight into brain dynamics. Some artefacts due to simultaneous acquisition pose a threat to the quality of the data. One such problematic artefact is the ballistocardiogram (BCG) artefact.

    METHODS: We developed a hybrid algorithm that combines features of empirical mode decomposition (EMD) with principal component analysis (PCA) to reduce the BCG artefact. The algorithm does not require extra electrocardiogram (ECG) or electrooculogram (EOG) recordings to extract the BCG artefact.

    RESULTS: The method was tested with both simulated and real EEG data of 11 participants. From the simulated data, the similarity index between the extracted BCG and the simulated BCG showed the effectiveness of the proposed method in BCG removal. On the other hand, real data were recorded with two conditions, i.e. resting state (eyes closed dataset) and task influenced (event-related potentials (ERPs) dataset). Using qualitative (visual inspection) and quantitative (similarity index, improved normalized power spectrum (INPS) ratio, power spectrum, sample entropy (SE)) evaluation parameters, the assessment results showed that the proposed method can efficiently reduce the BCG artefact while preserving the neuronal signals.

    COMPARISON WITH EXISTING METHODS: Compared with conventional methods, namely, average artefact subtraction (AAS), optimal basis set (OBS) and combined independent component analysis and principal component analysis (ICA-PCA), the statistical analyses of the results showed that the proposed method has better performance, and the differences were significant for all quantitative parameters except for the power and sample entropy.

    CONCLUSIONS: The proposed method does not require any reference signal, prior information or assumption to extract the BCG artefact. It will be very useful in circumstances where the reference signal is not available.

    MeSH terms: Adult; Algorithms*; Analysis of Variance; Attention/physiology; Computer Simulation; Electroencephalography/methods*; Evoked Potentials; Heart/physiology; Humans; Magnetic Resonance Imaging/methods*; Models, Neurological; Motion Perception/physiology; Neuropsychological Tests; Rest; Visual Perception/physiology; Artifacts*; Principal Component Analysis; Young Adult; Multimodal Imaging/methods*
  5. Rehman IU, Bukhsh A, Khan TM
    Travel Med Infect Dis, 2017 Jul-Aug;18:67-69.
    PMID: 28842213 DOI: 10.1016/j.tmaid.2017.08.002
    World Health Organization (WHO) measles surveillance data report a reduction in cases of measles globally from 67,524 cases in 2015 to 16,846 in 2016, and a reduction in deaths from 546,800 to 114,900 during period of 2000-14. Pakistan is among the five nations where almost a million children did not receive their first dose of measles vaccination, and outbreaks of the disease resulted in 4386 cases in 2011, 14,687 cases in 2012 with 310 deaths. In 2013, about 25,401 cases of measles were reported and 321 affected children died. The measles vaccination coverage is very low in Pakistan for both 1st dose and booster dose. To prevent outbreaks of measles in Pakistan a national vaccination program should be launched side by side with a polio eradication program in each district and township and a campaign should be launched to educate parents on measles vaccination for childrens to reduce the measles case fatality rate.
    MeSH terms: Child; Humans; Immunization/statistics & numerical data*; Measles Vaccine*; Pakistan/epidemiology
  6. Parate D, Franco-Obregón A, Fröhlich J, Beyer C, Abbas AA, Kamarul T, et al.
    Sci Rep, 2017 08 25;7(1):9421.
    PMID: 28842627 DOI: 10.1038/s41598-017-09892-w
    Pulse electromagnetic fields (PEMFs) have been shown to recruit calcium-signaling cascades common to chondrogenesis. Here we document the effects of specified PEMF parameters over mesenchymal stem cells (MSC) chondrogenic differentiation. MSCs undergoing chondrogenesis are preferentially responsive to an electromagnetic efficacy window defined by field amplitude, duration and frequency of exposure. Contrary to conventional practice of administering prolonged and repetitive exposures to PEMFs, optimal chondrogenic outcome is achieved in response to brief (10 minutes), low intensity (2 mT) exposure to 6 ms bursts of magnetic pulses, at 15 Hz, administered only once at the onset of chondrogenic induction. By contrast, repeated exposures diminished chondrogenic outcome and could be attributed to calcium entry after the initial induction. Transient receptor potential (TRP) channels appear to mediate these aspects of PEMF stimulation, serving as a conduit for extracellular calcium. Preventing calcium entry during the repeated PEMF exposure with the co-administration of EGTA or TRP channel antagonists precluded the inhibition of differentiation. This study highlights the intricacies of calcium homeostasis during early chondrogenesis and the constraints that are placed on PEMF-based therapeutic strategies aimed at promoting MSC chondrogenesis. The demonstrated efficacy of our optimized PEMF regimens has clear clinical implications for future regenerative strategies for cartilage.
    MeSH terms: Calcium/metabolism; Cell Differentiation/radiation effects; Cells, Cultured; Dose-Response Relationship, Radiation; Electromagnetic Fields*; Extracellular Matrix; Humans; Calcium Signaling; Chondrogenesis/radiation effects*; Mesenchymal Stromal Cells/cytology; Mesenchymal Stromal Cells/metabolism*; Mesenchymal Stromal Cells/radiation effects*
  7. Dianawati D, Lim SF, Ooi YBH, Shah NP
    J Food Sci, 2017 Sep;82(9):2134-2141.
    PMID: 28843042 DOI: 10.1111/1750-3841.13820
    The aims of this study were to evaluate the effect of types of protein-based microcapsules and storage at various ambient temperatures on the survival of Lactobacillus acidophilus during exposure to simulated gastrointestinal tract and on the change in thermo-tolerance during heating treatment. The encapsulating materials were prepared using emulsions of protein (sodium caseinate, soy protein isolate, or pea protein), vegetable oil, and glucose, with maltodextrin was used as a wall material. The formulations were heated at 90 °C for 30 min to develop Maillard substances prior to being incorporated with L. acidophilus. The mixtures were then spray dried. The microspheres were stored at 25, 30, and 35 °C for 8 wk and examined every 4 wk. The addition of proteins as encapsulating materials demonstrated a significant protective effect (P < 0.05) as compared to the control sample. Sodium caseinate and soy protein isolate appeared more effective than pea protein in protecting the bacteria after spray drying and during the storage at different room temperatures. Storage at 35 °C resulted in a significant decrease in survival at end of storage period regardless the type of encapsulating materials. The addition of protein-based materials also enhanced the survival of L. acidophilus during exposure to simulated gastrointestinal condition as compared to the control. After spray drying and after 0th wk storage, casein, soy protein isolate, and pea protein-based formulations protected the bacteria during heat treatment. In fact, a significant decrease in thermal tolerance was inevitable after 2 wk of storage at 25 °C.
    MeSH terms: Capsules; Caseins; Emulsions; Glucose; Hot Temperature; Heating; Lactobacillus acidophilus; Microspheres; Polysaccharides; Temperature; Probiotics; Soybean Proteins; Gastrointestinal Tract
  8. Tan GJ, Sulong G, Rahim MSM
    Forensic Sci Int, 2017 Oct;279:41-52.
    PMID: 28843097 DOI: 10.1016/j.forsciint.2017.07.034
    This paper presents a review on the state of the art in offline text-independent writer identification methods for three major languages, namely English, Chinese and Arabic, which were published in literatures from 2011 till 2016. For ease of discussions, we grouped the techniques into three categories: texture-, structure-, and allograph-based. Results are analysed, compared and tabulated along with datasets used for fair and just comparisons. It is observed that during that period, there are significant progresses achieved on English and Arabic; however, the growth on Chinese is rather slow and far from satisfactory in comparison to its wide usage. This is due to its complex writing structure. Meanwhile, issues on datasets used by previous studies are also highlighted because the size matter - accuracy of the writer identification deteriorates as database size increases.
    MeSH terms: Biological Phenomena; Language; Publications; Writing; Databases, Factual
  9. Plusquin M, Guida F, Polidoro S, Vermeulen R, Raaschou-Nielsen O, Campanella G, et al.
    Environ Int, 2017 11;108:127-136.
    PMID: 28843141 DOI: 10.1016/j.envint.2017.08.006
    Long-term exposure to air pollution has been associated with several adverse health effects including cardiovascular, respiratory diseases and cancers. However, underlying molecular alterations remain to be further investigated. The aim of this study is to investigate the effects of long-term exposure to air pollutants on (a) average DNA methylation at functional regions and, (b) individual differentially methylated CpG sites. An assumption is that omic measurements, including the methylome, are more sensitive to low doses than hard health outcomes. This study included blood-derived DNA methylation (Illumina-HM450 methylation) for 454 Italian and 159 Dutch participants from the European Prospective Investigation into Cancer and Nutrition (EPIC). Long-term air pollution exposure levels, including NO2, NOx, PM2.5, PMcoarse, PM10, PM2.5 absorbance (soot) were estimated using models developed within the ESCAPE project, and back-extrapolated to the time of sampling when possible. We meta-analysed the associations between the air pollutants and global DNA methylation, methylation in functional regions and epigenome-wide methylation. CpG sites found differentially methylated with air pollution were further investigated for functional interpretation in an independent population (EnviroGenoMarkers project), where (N=613) participants had both methylation and gene expression data available. Exposure to NO2 was associated with a significant global somatic hypomethylation (p-value=0.014). Hypomethylation of CpG island's shores and shelves and gene bodies was significantly associated with higher exposures to NO2 and NOx. Meta-analysing the epigenome-wide findings of the 2 cohorts did not show genome-wide significant associations at single CpG site level. However, several significant CpG were found if the analyses were separated by countries. By regressing gene expression levels against methylation levels of the exposure-related CpG sites, we identified several significant CpG-transcript pairs and highlighted 5 enriched pathways for NO2 and 9 for NOx mainly related to the immune system and its regulation. Our findings support results on global hypomethylation associated with air pollution, and suggest that the shores and shelves of CpG islands and gene bodies are mostly affected by higher exposure to NO2 and NOx. Functional differences in the immune system were suggested by transcriptome analyses.
    MeSH terms: Air Pollutants/analysis; Air Pollutants/pharmacology*; Cardiovascular Diseases/chemically induced; Environmental Exposure/analysis; Female; Humans; Male; Middle Aged; Prospective Studies; Cohort Studies; Gene Expression; DNA Methylation/drug effects*; European Continental Ancestry Group; Particulate Matter/analysis; Soot/analysis; Genome-Wide Association Study; Epigenomics
  10. Peltzer K, Pengpid S, Sychareun V, Ferrer AJG, Low WY, Huu TN, et al.
    BMC Cardiovasc Disord, 2017 08 23;17(1):230.
    PMID: 28835205 DOI: 10.1186/s12872-017-0666-3
    BACKGROUND: Existing evidence suggests that the cardiovascular morbidities are increasing among pre-hypertensive individuals compared to normal. The aim of this study was to evaluate the prevalence of prehypertension, hypertension and to identify psychosocial risk factors for prehypertension among university students in Association of South East Asian Nation (ASEAN) countries.
    METHODS: Based on a cross-sectional survey, the total sample included 4649 undergraduate university students (females = 65.3%; mean age 20.5, SD = 2.9, age range of 18-30 years) from 7 ASEAN countries (Indonesia, Laos, Malaysia, Myanmar, Philippines, Thailand and Vietnam). Blood pressure, anthropometric, health behaviour and psychosocial variables were measured.
    RESULTS: Overall, 19.0% of the undergraduate university students across ASEAN countries had prehypertension, 6.7% hypertension and 74.2% were normotensives. There was country variation in prehypertension prevalence, ranging from 11.3% in Indonesia and 11.5% in Malaysia to above 18% in Laos, Myanmar and Thailand. In multivariate analysis, sociodemographic variables (male gender, living in an upper middle income country, and living on campus or off campus on their own), nutrition and weight variables (not being underweight and obese, having once or more times soft drinks in a day and never or rarely having chocolate or candy), heavy drinking and having depressive symptoms were associated with prehypertension.
    CONCLUSION: The study found a high prevalence of prehypertension in ASEAN university students. Several psychosocial risk factors including male gender, obesity, soft drinks consumption, heavy drinking and depression symptoms have been identified which can help in intervention programmes.
    MeSH terms: Alcohol Drinking in College/ethnology; Alcohol Drinking in College/psychology; Adolescent; Adult; Asia/epidemiology; Carbonated Beverages/adverse effects; Cross-Sectional Studies; Depression/ethnology; Depression/psychology; Feeding Behavior/ethnology; Female; Health Status; Humans; Malaysia; Male; Obesity/ethnology; Obesity/psychology; Risk Factors; Sex Factors; Students; Students/psychology*; Universities*; Prevalence; Multivariate Analysis; Risk Assessment; Asian Continental Ancestry Group/psychology*; Young Adult; Prehypertension/diagnosis; Prehypertension/ethnology*; Prehypertension/psychology*
  11. Chin KY, Ima-Nirwana S
    Int J Endocrinol Metab, 2017 Jan;15(1):e43053.
    PMID: 28835763 DOI: 10.5812/ijem.43053
    BACKGROUND: Testosterone deficiency is linked to low-grade inflammation in humans, but this condition is not replicated in an animal study. The current study aims at determining the effects of testosterone deficiency and its replacement on the circulating inflammatory cytokine level in orchidectomized male rats.

    METHODS: Three-month-old Sprague-Dawley male rats (n = 18) were randomized equally into 3 groups. Bilateral orchidectomy was performed on 2 groups. The sham group was subjected to similar surgical stress, but their testes were retained. One of the orchidectomized groups received intramuscular injection of 7 mg/kg testosterone enanthate suspended in peanut oil weekly and the other 2 groups received equivolume of peanut oil injection. After 8 weeks, the rats were sacrificed and their blood was collected for the analysis of the levels of inflammatory cytokines and testosterone.

    RESULTS: Testosterone level was significantly lower in the untreated orchidectomized group compared to the sham group. Testosterone replacement significantly increased the level of testosterone in the orchidectomized rats compared to the sham and untreated orchidectomized rats. Interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), and tumor necrosis factor alpha (TNFα) showed an increasing trend in orchidectomized rats, albeit not statistically significant. Interleukin-6 (IL-6) level increased significantly in the orchidectomized group compared to the sham group. Testosterone replacement at the supraphysiological dose did not alter the level of inflammatory cytokines significantly in orchidectomized rats.

    CONCLUSIONS: Testosterone deficiency can elicit a state of low-grade inflammation, shown by an increase in interleukin-6 level, but exogenous supraphysiological testosterone replacement does not suppress the inflammation.

  12. Puebla-Osorio N, Sarchio SNE, Ullrich SE, Byrne SN
    Methods Mol Biol, 2017;1627:213-222.
    PMID: 28836204 DOI: 10.1007/978-1-4939-7113-8_14
    Mast cells are part of the immune system and characteristically contain histamine- and heparin-rich basophilic granules. While these cells are usually associated with allergy and anaphylaxis, they also promote wound healing and angiogenesis and confer protection against pathogens. The presence of these cells is sometimes indicative of a poor prognosis, especially in skin cancer, pancreatic cancer, and lymphoma. Toluidine blue staining of acid-fast granules is an established method for the identification and quantification of mast cells. Generating detailed information on the location of mast cells within tissues is problematic using this technique and often requires serial sections from adjacent tissue to be separately stained with hematoxylin and eosin (H&E). Staining serial sections is not always possible, particularly if the sample is very small or rare. In such cases, a method of simultaneously identifying and localizing mast cells in a tissue would be advantageous. Toluidine blue and H&E are not commonly combined because H&E includes repetitive washes in water, which may affect the efficacy of the aqueous-soluble toluidine blue. We have developed and tested a novel staining technique that integrates toluidine blue between hematoxylin and eosin in one simple procedure. This protocol works on both frozen and formalin-fixed, paraffin-embedded tissue and readily allows for the identification of purple-stained mast cells against a clean H&E background. This facilitates a more accurate localization and proper counting of mast cells in normal and affected tissue.
    MeSH terms: Animals; Eosine Yellowish-(YS); Hematoxylin; Mast Cells/metabolism*; Mast Cells/pathology*; Tolonium Chloride*; Mice, Knockout; Mice
  13. Martin CJ, Vassileva J, Vano E, Mahesh M, Ebdon-Jackson S, Ng K, et al.
    J Radiol Prot, 2017 Aug 24.
    PMID: 28836506 DOI: 10.1088/1361-6498/aa881e
    This paper sets out guidelines for managing radiation exposure incidents involving patients in diagnostic and interventional radiology. The work is based on collation of experiences from representatives of international and national organizations for radiologists, medical physicists, radiographers, regulators, and equipment manufacturers, derived from an International Atomic Energy Agency Technical Meeting. More serious overexposures can result in skin doses high enough to produce tissue reactions, in interventional procedures and computed tomography, most notably from perfusion studies. A major factor involved has been deficiencies in training of staff in operation of equipment and optimization techniques. The use of checklists and time outs before procedures commence, and dose alerts when critical levels are reached during procedures can provide safeguards to reduce risks of these effects occurring. However, unintended and accidental overexposures resulting in relatively small additional doses can take place in any diagnostic or interventional X-ray procedure and it is important to learn from errors that occur, as these may lead to increased risks of stochastic effects. Such events may involve the wrong examinations, procedural errors, or equipment faults. Guidance is given on prevention, investigation and dose calculation for radiology exposure incidents within healthcare facilities. Responsibilities should be clearly set out in formal policies, and procedures should be in place to ensure that root causes are identified and deficiencies addressed. When an overexposure of a patient or an unintended exposure of a foetus occurs, the foetal, organ, skin and/or effective dose may be estimated from exposure data. When doses are very low, generic values for the examination may be sufficient, but a full assessment of doses to all exposed organs and tissues may sometimes be required. The use of general terminology to describe risks from stochastic effects is recommended rather than calculation of numerical values, as these are misleading when applied to individuals.
  14. Almahbashi T, Aljunid SM, Ismail A
    East Mediterr Health J, 2017 Aug 20;23(6):415-421.
    PMID: 28836654
    It is important to link health professional education to the health service needs of the private and public labour market so as to meet the plans of the health sector. Thus, the main focus of this study was to identify the present labour market requirements for the outcomes of health training institutes. A qualitative study was carried out among mixed healthcare professionals and various stakeholders in Sana'a City, Yemen. Six focus group discussions were formed for 42 graduates and 20 in-depth interviews were undertaken with health development partners and public and private employers. Outcomes of the health training institutes were still below the expectations of the health labour market, and did not fill the existing gaps in English-language proficiency and clinical skills. The survival of health professional education depends on future development to meet labour market demands through collaboration between key stakeholders, regular updating of the curriculum, and constant professional development of the teaching staff.
    MeSH terms: Adult; Curriculum; Education, Professional/organization & administration*; Female; Health Personnel/education*; Humans; Male; Yemen; Focus Groups; Qualitative Research
  15. Kamaruzaman S, Sanagi MM, Yahaya N, Wan Ibrahim WA, Endud S, Wan Ibrahim WN
    J Sep Sci, 2017 Nov;40(21):4222-4233.
    PMID: 28837263 DOI: 10.1002/jssc.201700549
    A new facile magnetic micro-solid-phase extraction coupled to gas chromatography and mass spectrometry detection was developed for the extraction and determination of selected antidepressant drugs in biological fluids using magnetite-MCM-41 as adsorbent. The synthesized sorbent was characterized by several spectroscopic techniques. The maximum extraction efficiency for extraction of 500 μg/L antidepressant drugs from aqueous solution was obtained with 15 mg of magnetite-MCM-41 at pH 12. The analyte was desorbed using 100 μL of acetonitrile prior to gas chromatography determination. This method was rapid in which the adsorption procedure was completed in 60 s. Under the optimized conditions using 15 mL of antidepressant drugs sample, the calibration curve showed good linearity in the range of 0.05-500 μg/L (r2  = 0.996-0.999). Good limits of detection (0.008-0.010 μg/L) were obtained for the analytes with good relative standard deviations of <8.0% (n = 5) for the determination of 0.1, 5.0, and 500.0 μg/L of antidepressant drugs. This method was successfully applied to the determination of amitriptyline and chlorpromazine in plasma and urine samples. The recoveries of spiked plasma and urine samples were in the range of 86.1-115.4%. Results indicate that magnetite micro-solid-phase extraction with gas chromatography and mass spectrometry is a convenient, fast, and economical method for the extraction and determination of amitriptyline and chlorpromazine in biological samples.
    MeSH terms: Amitriptyline/blood; Amitriptyline/urine; Antidepressive Agents/blood*; Antidepressive Agents/urine*; Chlorpromazine/blood; Chlorpromazine/urine; Humans; Gas Chromatography-Mass Spectrometry; Silicon Dioxide*; Ferrosoferric Oxide*; Solid Phase Extraction; Magnetite Nanoparticles
  16. Baba Ismail YM, Ferreira AM, Bretcanu O, Dalgarno K, El Haj AJ
    Colloids Surf B Biointerfaces, 2017 Nov 01;159:445-453.
    PMID: 28837894 DOI: 10.1016/j.colsurfb.2017.07.086
    This paper presents a new approach in assembling bone extracellular matrix components onto PLA films, and investigates the most favourable environment which can be created using the technique for cell-material interactions. Poly (lactic acid) (PLA) films were chemically modified by covalently binding the poly(ethylene imine) (PEI) as to prepare the substrate for immobilization of polyelectrolyte multilayers (PEMs) coating. Negatively charged polyelectrolyte consists of well-dispersed silicon-carbonated hydroxyapatite (SiCHA) nanopowders in hyaluronic acid (Hya) was deposited onto the modified PLA films followed by SiCHA in collagen type I as the positively charged polyelectrolyte. The outermost layer was finally cross-linked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrocholoride and N-hydroxysulfosuccinimide sodium salt (EDC/NHS) solutions. The physicochemical features of the coated PLA films were monitored via X-ray Photoelectron Spectroscopy (XPS) and Atomic Force Microscope (AFM). The amounts of calcium and collagen deposited on the surface were qualitatively and quantitatively determined. The surface characterizations suggested that 5-BL has the optimum surface roughness and highest amounts of calcium and collagen depositions among tested films. In vitro human mesenchymal stem cells (hMSCs) cultured on the coated PLA films confirmed that the coating materials greatly improved cell attachment and survival compared to unmodified PLA films. The cell viability, cell proliferation and Alkaline Phosphatase (ALP) expression on 5-BL were found to be the most favourable of the tested films. Hence, this newly developed coating materials assembly could contribute to the improvement of the bioactivity of polymeric materials and structures aimed to bone tissue engineering applications.
    MeSH terms: Hyaluronic Acid/chemistry; Imines/chemistry; Materials Testing/methods*; Membranes, Artificial*; Polyesters/chemistry*; Polyethylenes/chemistry; Microscopy, Atomic Force; Coated Materials, Biocompatible/chemistry*; Photoelectron Spectroscopy
  17. Azhar B, Saadun N, Prideaux M, Lindenmayer DB
    J Environ Manage, 2017 Dec 01;203(Pt 1):457-466.
    PMID: 28837912 DOI: 10.1016/j.jenvman.2017.08.021
    Most palm oil currently available in global markets is sourced from certified large-scale plantations. Comparatively little is sourced from (typically uncertified) smallholders. We argue that sourcing sustainable palm oil should not be determined by commercial certification alone and that the certification process should be revisited. There are so-far unrecognized benefits of sourcing palm oil from smallholders that should be considered if genuine biodiversity conservation is to be a foundation of 'environmentally sustainable' palm oil production. Despite a lack of certification, smallholder production is often more biodiversity-friendly than certified production from large-scale plantations. Sourcing palm oil from smallholders also alleviates poverty among rural farmers, promoting better conservation outcomes. Yet, certification schemes - the current measure of 'sustainability' - are financially accessible only for large-scale plantations that operate as profit-driven monocultures. Industrial palm oil is expanding rapidly in regions with weak environmental laws and enforcement. This warrants the development of an alternative certification scheme for smallholders. Greater attention should be directed to deforestation-free palm oil production in smallholdings, where production is less likely to cause large scale biodiversity loss. These small-scale farmlands in which palm oil is mixed with other crops should be considered by retailers and consumers who are interested in promoting sustainable palm oil production. Simultaneously, plantation companies should be required to make their existing production landscapes more compatible with enhanced biodiversity conservation.
    MeSH terms: Agriculture; Conservation of Natural Resources; Food Supply*; Plant Oils*; Arecaceae; Biodiversity*
  18. Teoh Y, Wallis E, Stephen ID, Mitchell P
    Cognition, 2017 02;159:48-60.
    PMID: 27886521 DOI: 10.1016/j.cognition.2016.11.003
    Past research tells us that individuals can infer information about a target's emotional state and intentions from their facial expressions (Frith & Frith, 2012), a process known as mentalising. This extends to inferring the events that caused the facial reaction (e.g. Pillai, Sheppard, & Mitchell, 2012; Pillai et al., 2014), an ability known as retrodictive mindreading. Here, we enter new territory by investigating whether or not people (perceivers) can guess a target's social context by observing their response to stimuli. In Experiment 1, perceivers viewed targets' responses and were able to determine whether these targets were alone or observed by another person. In Experiment 2, another group of perceivers, without any knowledge of the social context or what the targets were watching, judged whether targets were hiding or exaggerating their facial expressions; and their judgments discriminated between conditions in which targets were observed and alone. Experiment 3 established that another group of perceivers' judgments of social context were associated with estimations of target expressivity to some degree. In Experiments 1 and 2, the eye movements of perceivers also varied between conditions in which targets were observed and alone. Perceivers were thus able to infer a target's social context from their visible response. The results demonstrate an ability to use other minds as a window onto a social context that could not be seen directly.
    MeSH terms: Facial Recognition*; Adolescent; Adult; Emotions*; Eye Movements; Facial Expression*; Female; Humans; Judgment*; Male; Social Behavior; Social Perception*; Young Adult; Theory of Mind*
  19. Mohd Nor MN, Sabaratnam V, Tan GYA
    Int J Syst Evol Microbiol, 2017 Apr;67(4):851-855.
    PMID: 27902276 DOI: 10.1099/ijsem.0.001683
    A bacterial isolate, designated strain S37T, was isolated from the rhizosphere of oil palm (Elaeis guineensis). Strain S37T was found to be Gram-stain-negative, aerobic, motile and rod shaped. Based on 16S rRNA gene sequence analysis, strain S37T was most closely related to Devosia albogilva IPL15T (97.3 %), Devosia chinhatensis IPL18T (96.8 %) and Devosia subaequoris HST3-14T (96.5 %). The G+C content of the genomic DNA was 63.0 mol%, and dominant cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), 11-methyl C18 : 1ω7c and C16 : 0. The predominant isoprenoid quinone was ubiquinone-10 (Q-10), and the major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, glycolipid and phospholipids. Based on the polyphasic taxonomic data, it is clear that strain S37T represents a novel species of the genus Devosia within the family Hyphomicrobiaceae, for which we propose the name Devosia elaeis sp. nov., with strain S37T (=TBRC 5145T=LMG 29420T) as the type strain.
    MeSH terms: Base Composition; DNA, Bacterial/genetics; Fatty Acids/chemistry; Glycolipids/chemistry; Malaysia; Phospholipids/chemistry; Phylogeny*; RNA, Ribosomal, 16S/genetics; Soil Microbiology*; Ubiquinone/chemistry; Bacterial Typing Techniques; Sequence Analysis, DNA; Arecaceae/microbiology*; Hyphomicrobiaceae/classification*; Hyphomicrobiaceae/genetics; Hyphomicrobiaceae/isolation & purification; Rhizosphere*
  20. Thent ZC, Zaidun NH, Azmi MF, Senin MI, Haslan H, Salehuddin R
    Curr Drug Targets, 2017;18(6):734-750.
    PMID: 27919208 DOI: 10.2174/1389450118666161205125548
    Colorectal cancer (CRC) remains one of the major leading causes of cancer related morbidity and mortality. Apart from the conventional anti-neoplastic agents, metformin, a biguanide anti-diabetic agent, has recently found to have anti-cancer property. Several studies observed the effect of metformin towards its anti-cancer effect on colon or colorectal cancer in diabetic patients. However, only a few studies showed its effect on colorectal cancer in relation to the non-diabetic status. The present review aimed to highlight the insight into the molecular pathway of metformin towards colorectal cancer in the absence of diabetes mellitus. In CRC-independent of diabetes mellitus, highly deregulation of PI3K/AKT pathway is found which activates the downstream mammalian target of rapamycin (mTOR). Metformin inhibits cancer growth in colon by suppressing the colonic epithelial proliferation by inhibiting the mTOR pathway. Metformin exerts its anti-neoplastic effects by acting on tumour suppressor pathway via activating the adenosine monophosphate.activated protein kinase (AMPK) signaling pathway. Metformin interrupts the glucose metabolism by activating the AMPK. Metformin reduces tumour cell growth and metastasis by activating the p53 tumour suppressor gene. In addition to its therapeutic benefits, metformin is easily accessible, cost effective with better tolerance to the patients compared to the chemotherapeutic agents. This review summarised modern findings on the therapeutic applications of metformin on the colorectal cancer with no evidences of diabetes mellitus.
    MeSH terms: Animals; Antineoplastic Agents/pharmacology*; Antineoplastic Agents/therapeutic use; Cell Cycle/drug effects; Clinical Trials as Topic; Humans; Metformin/pharmacology*; Metformin/therapeutic use; Colorectal Neoplasms/drug therapy*; Colorectal Neoplasms/metabolism; Signal Transduction/drug effects; Gene Expression Regulation, Neoplastic/drug effects; Treatment Outcome; Cell Proliferation/drug effects; TOR Serine-Threonine Kinases/metabolism*
External Links