Browse publications by year: 2017

  1. Salimi N, Zilany MSA, Carney LH
    J Assoc Res Otolaryngol, 2017 Jun;18(3):441-456.
    PMID: 28097439 DOI: 10.1007/s10162-016-0612-6
    A phenomenological model of the responses of neurons in the superior paraolivary nucleus (SPON) of the rodent is presented in this study. Pure tones at the characteristic frequency (CF) and broadband noise stimuli evoke offset-type responses in these neurons. SPON neurons also phase-lock to the envelope of sinusoidally amplitude-modulated (SAM) stimuli for a range of modulation frequencies. Model SPON neuron received inhibitory input that was relayed by the ipsilateral medial nucleus of the trapezoid body from the contralateral model ventral cochlear nucleus neuron. The SPON model response was simulated by detecting the slope of its inhibitory postsynaptic potential. Responses of the proposed model to pure tones at CF and broadband noise were offset-type independent of the duration of the input stimulus. SPON model responses were also synchronized to the envelope of SAM stimuli with precise timing for a range of modulation frequencies. Modulation transfer functions (MTFs) obtained from the model response to SAM stimuli resemble the physiological MTFs. The output of the proposed SPON model provides an input for models of physiological responses at higher levels of the ascending auditory pathway and can also be utilized to infer possible mechanisms underlying gap detection and duration encoding as well as forward masking at the level of the auditory midbrain.
    MeSH terms: Animals; Inferior Colliculi/physiology*; Models, Neurological*; Superior Olivary Complex/physiology*
  2. Maji IK, Habibullah MS, Saari MY
    Environ Sci Pollut Res Int, 2017 Mar;24(8):7160-7176.
    PMID: 28097481 DOI: 10.1007/s11356-016-8326-1
    The paper examines the impacts of financial development on sectoral carbon emissions (CO2) for environmental quality in Malaysia. Since the financial sector is considered as one of the sectors that will contribute to Malaysian economy to become a developed country by 2020, we utilize a cointegration method to investigate how financial development affects sectoral CO2 emissions. The long-run results reveal that financial development increases CO2 emissions from the transportation and oil and gas sector and reduces CO2 emissions from manufacturing and construction sectors. However, the elasticity of financial development is not significant in explaining CO2 emissions from the agricultural sector. The results for short-run elasticities were also consistent with the long-run results. We conclude that generally, financial development increases CO2 emissions and reduces environmental quality in Malaysia.
    MeSH terms: Developing Countries; Malaysia; Economic Development*
  3. Yip CH
    J Surg Oncol, 2017 Apr;115(5):538-543.
    PMID: 28097656 DOI: 10.1002/jso.24560
    Metastatic breast cancer is an incurable disease. With improvement in systemic therapy, survival has improved over the past few years. Removing the primary tumor has shown improved survival in retrospective studies, but this may be due to selection bias. The first reported randomized controlled trial (RCT) from India showed no difference in survival with surgery. However another RCT from Turkey showed that a select group of patient with bone-only metastases have a survival benefit.
    MeSH terms: Breast Neoplasms/mortality; Breast Neoplasms/pathology*; Breast Neoplasms/surgery*; Female; Humans; Mastectomy; Neoplasm Metastasis; Palliative Care*; Quality of Life; Randomized Controlled Trials as Topic
  4. Ab Rahman AF, Md Sahak N, Ali AM
    Int J Pharm Pract, 2017 Feb;25(1):75-80.
    PMID: 28097717 DOI: 10.1111/ijpp.12336
    OBJECTIVES: Published nomograms to monitor extended-interval dosing (EID) gentamicin therapy were based on a fixed dose of 5 or 7 mg/kg. However, the average dose used for EID gentamicin regimen in our setting was about 3 mg/kg per day. We developed a new method of monitoring based on the duration of drug-free period (DFP) in a 24-h dosing interval.

    METHODS: Hospitalised adult patients on EID gentamicin were selected. We considered a DFP of between 2 and 8 h as appropriate. Data from two blood samples (2 and 6 h postdose) from each patient were used to estimate the duration of DFP (i.e. DFP method 1). DFP was also calculated for the same patient using an empirically estimated elimination rate constant (Ke ) and the same 6 h postdose concentration value (DFP method 2). Correlation between the two methods was made. An alternative graphical method to estimate DFP was attempted.

    KEY FINDINGS: Correlation between Ke and age was favourable (r = -0.453; P = 0.001). Ke derived from this empirical relationship was used to estimate DFP method 2. DFP method 1 correlated well with DFP method 2 (r = 0.742; P 

    MeSH terms: Adolescent; Adult; Age Factors; Aged; Aged, 80 and over; Anti-Bacterial Agents/administration & dosage; Anti-Bacterial Agents/blood; Data Display*; Drug Administration Schedule*; Female; Gentamicins/administration & dosage*; Gentamicins/blood; Gentamicins/pharmacokinetics*; Humans; Male; Middle Aged; Drug Monitoring/methods*; Young Adult
  5. Liu X, Lu D, Saw WY, Shaw PJ, Wangkumhang P, Ngamphiw C, et al.
    Eur J Hum Genet, 2017 04;25(4):499-508.
    PMID: 28098149 DOI: 10.1038/ejhg.2016.181
    The Asian Diversity Project (ADP) assembled 37 cosmopolitan and ethnic minority populations in Asia that have been densely genotyped across over half a million markers to study patterns of genetic diversity and positive natural selection. We performed population structure analyses of the ADP populations and divided these populations into four major groups based on their genographic information. By applying a highly sensitive algorithm haploPS to locate genomic signatures of positive selection, 140 distinct genomic regions exhibiting evidence of positive selection in at least one population were identified. We examined the extent of signal sharing for regions that were selected in multiple populations and observed that populations clustered in a similar fashion to that of how the ancestry clades were phylogenetically defined. In particular, populations predominantly located in South Asia underwent considerably different adaptation as compared with populations from the other geographical regions. Signatures of positive selection present in multiple geographical regions were predicted to be older and have emerged prior to the separation of the populations in the different regions. In contrast, selection signals present in a single population group tended to be of lower frequencies and thus can be attributed to recent evolutionary events.
    MeSH terms: Asia; Genotype; Humans; Population/genetics*; Selection, Genetic*; Genetic Variation*; Evolution, Molecular; Asian Continental Ancestry Group/genetics*
  6. Cheah HY, Šarenac O, Arroyo JJ, Vasić M, Lozić M, Glumac S, et al.
    Nanotoxicology, 2017 03;11(2):210-222.
    PMID: 28098511 DOI: 10.1080/17435390.2017.1285071
    Conjugation of Doxorubicin (DOX) to N-(2-hydroxypropyl) methylacrylamide copolymer (HPMA) has significantly reduced the DOX-associated cardiotoxicity. However, the reports on the impact of HPMA-DOX conjugates on the cardiovascular system such as blood pressure (BP) and heart rate (HR) were in restrained animals using tail cuff and/or other methods that lacked the resolution and sensitivity. Herein, we employed radiotelemetric-spectral-echocardiography approach to further understand the in vivo cardiovascular hemodynamics and variability post administration of free DOX and HPMA-DOX. Rats implanted with radio-telemetry device were administered intravenously with DOX (5 mg/kg), HPMA-DOX (5 mg DOX equivalent/kg) and HPMA copolymer and subjected to continuous cardiovascular monitoring and echocardiography for 140 days. We found that DOX-treated rats had ruffled fur, reduced body weight (BW) and a low survival rate. Although BP and HR were normal, spectral analysis indicated that their BP and HR variabilities were reduced. All rats exhibited typical signs of cardiotoxicity at histopathology. In contrast, HPMA-DOX rats gained weight over time and survived. Although BP, HR and related variabilities were unaffected, the left ventricular end diastolic volume (EDV) of these rats, as well as of the HPMA copolymer-treated rats, was found increased at the end of observation period. Additionally, HPMA copolymer caused microscopic injury of the heart tissue. All of these suggest the necessity of caution when employing HPMA as carrier for prolonged drug delivery. The current study also indicates the potential of radiotelemetric-spectral-echocardiography approach for improved preclinical cardiovascular risk assessment of polymer-drug conjugate and other nano-sized-drug constructs.
    MeSH terms: Acrylamides/administration & dosage; Acrylamides/toxicity*; Animals; Blood Pressure/drug effects*; Doxorubicin/administration & dosage; Doxorubicin/toxicity*; Echocardiography; Heart/drug effects*; Heart Rate/drug effects*; Injections, Intravenous; Male; Myocardium/pathology; Random Allocation; Telemetry; Survival Analysis; Rats, Wistar; Apoptosis/drug effects; Mice; Rats; Kaplan-Meier Estimate; Cardiotoxicity
  7. Phan CW, David P, Sabaratnam V
    J Med Food, 2017 Jan;20(1):1-10.
    PMID: 28098514 DOI: 10.1089/jmf.2016.3740
    There is an exponential increase in dementia in old age at a global level because of increasing life expectancy. The prevalence of neurodegenerative diseases such as dementia and Alzheimer's disease (AD) will continue to rise steadily, and is expected to reach 42 million cases worldwide in 2020. Despite the advancement of medication, the management of these diseases remains largely ineffective. Therefore, it is vital to explore novel nature-based nutraceuticals to mitigate AD and other age-related neurodegenerative disorders. Mushrooms and their extracts appear to hold many health benefits, including immune-modulating effects. A number of edible mushrooms have been shown to contain rare and exotic compounds that exhibit positive effects on brain cells both in vitro and in vivo. In this review, we summarize the scientific information on edible and culinary mushrooms with regard to their antidementia/AD active compounds and/or pharmacological test results. The bioactive components in these mushrooms and the underlying mechanism of their activities are discussed. In short, these mushrooms may be regarded as functional foods for the mitigation of neurodegenerative diseases.
    MeSH terms: Agaricales/metabolism; Agaricales/chemistry*; Animals; Humans; Plant Extracts/metabolism; Plant Extracts/pharmacology; Plant Extracts/chemistry*; Vegetables/metabolism; Vegetables/chemistry; Neurodegenerative Diseases/drug therapy; Neurodegenerative Diseases/metabolism; Functional Food/analysis*
  8. Chen YS, Wong JE, Ayob AF, Othman NE, Poh BK
    Nutrients, 2017 Jan 13;9(1).
    PMID: 28098770 DOI: 10.3390/nu9010062
    Mobile applications may improve dietary reporting among young adults due to their high accessibility and embedded camera function. This pilot study aimed to (i) evaluate users' acceptability and compliance in reporting dietary intake using a newly developed food diary mobile application (food app); and (ii) identify issues and recommendations for improving dietary assessment using this food app via quantitative and qualitative protocols. Twenty-eight university students each used a food app for seven consecutive days and attended one of five focus group interviews. A 42% decrement in reporting compliance was observed throughout the seven-day recording period. An average of 5.9 recording days were reported and 4.8 occasions of meal data were uploaded each day. Based on questionnaires, high levels of agreement were reported in terms of perceived usefulness (69.3%), perceived ease of use (77.1%), attitude (73.6%), perceived enjoyment (62.6%), and smartphone experience (91.1%), but such agreement was not reported for intention to use (38.1%) and social influence (33.4%). Four major themes emerged from the focus group interviews, namely, (i) features; (ii) potential use; (iii) utility issues of the food app; and (iv) suggestions for improvements. While the food app was well-accepted by most of the young adults, the current prototype would benefit from incorporation of a barcode scanning function, customizable reminders, in-app tutorial, an entertainment component, and enhancement in overall appearance.
    MeSH terms: Diet*; Female; Humans; Malaysia; Male; Patient Compliance; Pilot Projects; Surveys and Questionnaires; Nutrition Assessment; Diet Records*; Focus Groups; Qualitative Research; Cell Phones; Asian Continental Ancestry Group; Young Adult; Mobile Applications*
  9. Nasir J, Jamaluddin MH, Ahmad Khan A, Kamarudin MR, Yen BL, Owais O
    Sensors (Basel), 2017 Jan 13;17(1).
    PMID: 28098807 DOI: 10.3390/s17010148
    An L-shaped dual-band multiple-input multiple-output (MIMO) rectangular dielectric resonator antenna (RDRA) for long term evolution (LTE) applications is proposed. The presented antenna can transmit and receive information independently using fundamental TE111 and higher order TE121 modes of the DRA. TE111 degenerate mode covers LTE band 2 (1.85-1.99 GHz), 3 (1.71-1.88 GHz), and 9 (1.7499-1.7849 GHz) at fr = 1.8 GHz whereas TE121 covers LTE band 7 (2.5-2.69 GHz) at fr = 2.6 GHz, respectively. An efficient design method has been used to reduce mutual coupling between ports by changing the effective permittivity values of DRA by introducing a cylindrical air-gap at an optimal position in the dielectric resonator. This air-gap along with matching strips at the corners of the dielectric resonator keeps the isolation at a value more than 17 dB at both the bands. The diversity performance has also been evaluated by calculating the envelope correlation coefficient, diversity gain, and mean effective gain of the proposed design. MIMO performance has been evaluated by measuring the throughput of the proposed MIMO antenna. Experimental results successfully validate the presented design methodology in this work.
    MeSH terms: Equipment Design; Health Services; Chromosome Structures; Wireless Technology
  10. Chen TH, Yen AM, Fann JC, Gordon P, Chen SL, Chiu SY, et al.
    Medicine (Baltimore), 2017 Jan;96(3):e5684.
    PMID: 28099330 DOI: 10.1097/MD.0000000000005684
    BACKGROUND: The recent controversy about using mammography to screen for breast cancer based on randomized controlled trials over 3 decades in Western countries has not only eclipsed the paradigm of evidence-based medicine, but also puts health decision-makers in countries where breast cancer screening is still being considered in a dilemma to adopt or abandon such a well-established screening modality.

    METHODS: We reanalyzed the empirical data from the Health Insurance Plan trial in 1963 to the UK age trial in 1991 and their follow-up data published until 2015. We first performed Bayesian conjugated meta-analyses on the heterogeneity of attendance rate, sensitivity, and over-detection and their impacts on advanced stage breast cancer and death from breast cancer across trials using Bayesian Poisson fixed- and random-effect regression model. Bayesian meta-analysis of causal model was then developed to assess a cascade of causal relationships regarding the impact of both attendance and sensitivity on 2 main outcomes.

    RESULTS: The causes of heterogeneity responsible for the disparities across the trials were clearly manifested in 3 components. The attendance rate ranged from 61.3% to 90.4%. The sensitivity estimates show substantial variation from 57.26% to 87.97% but improved with time from 64% in 1963 to 82% in 1980 when Bayesian conjugated meta-analysis was conducted in chronological order. The percentage of over-detection shows a wide range from 0% to 28%, adjusting for long lead-time. The impacts of the attendance rate and sensitivity on the 2 main outcomes were statistically significant. Causal inference made by linking these causal relationships with emphasis on the heterogeneity of the attendance rate and sensitivity accounted for the variation in the reduction of advanced breast cancer (none-30%) and of mortality (none-31%). We estimated a 33% (95% CI: 24-42%) and 13% (95% CI: 6-20%) breast cancer mortality reduction for the best scenario (90% attendance rate and 95% sensitivity) and the poor scenario (30% attendance rate and 55% sensitivity), respectively.

    CONCLUSION: Elucidating the scenarios from high to low performance and learning from the experiences of these trials helps screening policy-makers contemplate on how to avoid errors made in ineffective studies and emulate the effective studies to save women lives.

    MeSH terms: Medical Overuse; Breast Neoplasms/diagnosis*; Breast Neoplasms/mortality; Humans; Mammography*; Mass Screening*; Patient Acceptance of Health Care; Sensitivity and Specificity; Randomized Controlled Trials as Topic
  11. Mohamed Rohani M, Calache H, Borromeo GL
    Aust Dent J, 2017 Jun;62(2):173-179.
    PMID: 27670933 DOI: 10.1111/adj.12465
    BACKGROUND: Special Needs Dentistry (SND) has been recognized as a dental specialty in Australia since 2003 but there have been no studies addressing the profile of patients for specialist care. The purpose of this study is to identify, via referrals received, the profile of patients and quality of referrals at the largest public SND unit in Victoria, Australia.
    METHODS: All referrals received over a 6-month period (1 January-30 June 2013) by the integrated SND unit (ISNU) were reviewed prior to allocation to the outpatient clinic (OP), domiciliary (DOM) or general anaesthetic (GA) services.
    RESULTS: Six-hundred and eighty-eight referrals were received with the majority for the OP clinic (68.3%), followed by DOM (22.4%) and GA services (9.3%) (χ2  = 360.2, P 
    MeSH terms: Adolescent; Adult; Aged; Australia; Developmental Disabilities/complications; Developmental Disabilities/epidemiology; Cross-Sectional Studies; Data Collection; Dental Clinics; Female; Disabled Persons*; Hospitals; Humans; Male; Middle Aged; Pediatric Dentistry/statistics & numerical data*; Quality of Health Care; Referral and Consultation/statistics & numerical data*; Time Factors; Victoria; Young Adult
  12. Mu D, Yuan D, Feng H, Xing F, Teo FY, Li S
    Mar Pollut Bull, 2017 Jan 30;114(2):705-714.
    PMID: 27802871 DOI: 10.1016/j.marpolbul.2016.10.056
    Sediment cores and overlying water samples were collected at four sites in Tianjin Coastal Zone, Bohai Bay, to investigate nutrient (N, P and Si) exchanges across the sediment-water interface. The exchange fluxes of each nutrient species were estimated based on the porewater profiles and laboratory incubation experiments. The results showed significant differences between the two methods, which implied that molecular diffusion alone was not the dominant process controlling nutrient exchanges at these sites. The impacts of redox conditions and bioturbation on the nutrient fluxes were confirmed by the laboratory incubation experiments. The results from this study showed that the nutrient fluxes measured directly from the incubation experiment were more reliable than that predicted from the porewater profiles. The possible impacts causing variations in the nutrient fluxes include sewage discharge and land reclamation.
    MeSH terms: China; Environmental Monitoring/methods*; Models, Theoretical*; Nitrogen/analysis; Phosphorus/analysis; Seasons; Seawater/chemistry*; Silicon/analysis; Water Pollutants, Chemical/analysis*; Geologic Sediments/chemistry*; Bays/chemistry*
  13. Norbäck D, Hashim JH, Hashim Z, Cai GH, Sooria V, Ismail SA, et al.
    Sci Total Environ, 2017 Jan 15;577:148-154.
    PMID: 27802882 DOI: 10.1016/j.scitotenv.2016.10.148
    Few health studies exist on dampness and mould in schools in the tropics. We studied associations between fraction of exhaled nitric oxide (FeNO), respiratory symptoms and airway infections among students and dampness and fungal DNA in schools in Malaysia. A total of 368 randomly selected students from 32 classrooms in 8 secondary schools in Penang, Malaysia, participated (58% participation rate). Information on current respiratory symptoms and the home environment was collected by a standardised questionnaire. FeNO was measured by NIOX MINO (50ml/min). The classrooms were inspected and dust was collected by vacuuming on special filters and was analysed for five fungal DNA sequences by quantitative PCR. Linear mixed models and 3-level multiple logistic regression (school, classroom, student) were applied adjusting for demographic data and the home environment. Totally 10.3% reported doctor's diagnosed asthma, 15.1% current wheeze, 12.4% current asthma, 37.3% daytime breathlessness, 10.2% nocturnal breathlessness, 38.9% airway infections and 15.5% had pollen or furry pet allergy. The geometric mean of FeNO was 19.9ppb and 45% had elevated FeNO (>20ppb). Boys had higher levels of FeNO. Chinese had less daytime breathlessness than Malay (OR=0.30: p<0.001). Indoor carbon dioxide levels were low (380-720ppm). Dampness was observed in 18% of the classrooms and was associated with respiratory infections (OR=3.70; 95% CI 1.14-12.1) and FeNO (p=0.04). Aspergillus versicolor DNA was detected in 67% of the classrooms. Higher numbers of Aspergillus versicolor DNA in classroom dust were associated with wheeze (p=0.006), current asthma (p=0.002), respiratory infections (p=0.005) and elevated FeNO levels (p=0.02). In conclusion, respiratory symptoms were common among the students and the high FeNO levels indicate ongoing airway inflammation. Building dampness and the mould Aspergillus versicolor in schools in Malaysia can be risk factors for impaired respiratory health among the students.
    MeSH terms: Adolescent; Asthma/epidemiology*; DNA, Fungal/analysis*; Dust; Female; Humans; Malaysia; Male; Nitric Oxide/analysis*; Respiratory Sounds; Schools; Students; Air Pollution, Indoor/analysis*
  14. Chua EG, Wise MJ, Khosravi Y, Seow SW, Amoyo AA, Pettersson S, et al.
    DNA Res, 2017 Feb 01;24(1):37-49.
    PMID: 27803027 DOI: 10.1093/dnares/dsw046
    Helicobacter pylori is a highly successful gastric pathogen. High genomic plasticity allows its adaptation to changing host environments. Complete genomes of H. pylori clinical isolate UM032 and its mice-adapted serial derivatives 298 and 299, generated using both PacBio RS and Illumina MiSeq sequencing technologies, were compared to identify novel elements responsible for host-adaptation. The acquisition of a jhp0562-like allele, which encodes for a galactosyltransferase, was identified in the mice-adapted strains. Our analysis implies a new β-1,4-galactosyltransferase role for this enzyme, essential for Ley antigen expression. Intragenomic recombination between babA and babB genes was also observed. Further, we expanded on the list of candidate genes whose expression patterns have been mediated by upstream homopolymer-length alterations to facilitate host adaption. Importantly, greater than four-fold reduction of mRNA levels was demonstrated in five genes. Among the down-regulated genes, three encode for outer membrane proteins, including BabA, BabB and HopD. As expected, a substantial reduction in BabA protein abundance was detected in mice-adapted strains 298 and 299 via Western analysis. Our results suggest that the expression of Ley antigen and reduced outer membrane protein expressions may facilitate H. pylori colonisation of mouse gastric epithelium.
    MeSH terms: Adaptation, Physiological*; Animals; Lipopolysaccharides/biosynthesis; Mutation; Quantum Theory*; Recombination, Genetic; Gene Expression*; Helicobacter pylori/genetics*; Helicobacter pylori/physiology; Genome, Bacterial; Mice; Host-Pathogen Interactions*
  15. Cirillo N, Hassona Y, Celentano A, Lim KP, Manchella S, Parkinson EK, et al.
    Carcinogenesis, 2017 01;38(1):76-85.
    PMID: 27803052 DOI: 10.1093/carcin/bgw113
    The interrelationship between malignant epithelium and the underlying stroma is of fundamental importance in tumour development and progression. In the present study, we used cancer-associated fibroblasts (CAFs) derived from genetically unstable oral squamous cell carcinomas (GU-OSCC), tumours that are characterized by the loss of genes such as TP53 and p16INK4A and with extensive loss of heterozygosity, together with CAFs from their more genetically stable (GS) counterparts that have wild-type TP53 and p16INK4A and minimal loss of heterozygosity (GS-OSCC). Using a systems biology approach to interpret the genome-wide transcriptional profile of the CAFs, we show that transforming growth factor-β (TGF-β) family members not only had biological relevance in silico but also distinguished GU-OSCC-derived CAFs from GS-OSCC CAFs and fibroblasts from normal oral mucosa. In view of the close association between TGF-β family members, we examined the expression of TGF-β1 and TGF-β2 in the different fibroblast subtypes and showed increased levels of active TGF-β1 and TGF-β2 in CAFs from GU-OSCC. CAFs from GU-OSCC, but not GS-OSCC or normal fibroblasts, induced epithelial-mesenchymal transition and down-regulated a broad spectrum of cell adhesion molecules resulting in epithelial dis-cohesion and invasion of target keratinocytes in vitro in a TGF-β-dependent manner. The results demonstrate that the TGF-β family of cytokines secreted by CAFs derived from genotype-specific oral cancer (GU-OSCC) promote, at least in part, the malignant phenotype by weakening intercellular epithelial adhesion.
    MeSH terms: Carcinoma, Squamous Cell/genetics; Carcinoma, Squamous Cell/metabolism; Carcinoma, Squamous Cell/pathology; Cell Adhesion; Cells, Cultured; Fibroblasts/metabolism; Fibroblasts/pathology; Genotype; Humans; Mouth Mucosa/metabolism; Mouth Mucosa/pathology; Mouth Neoplasms/genetics; Mouth Neoplasms/metabolism; Mouth Neoplasms/pathology*; Neoplasm Invasiveness; Keratinocytes/metabolism; Keratinocytes/pathology*; Gene Expression Regulation, Neoplastic*; Tumor Suppressor Protein p53/genetics; Loss of Heterozygosity; Cyclin-Dependent Kinase Inhibitor p16/genetics; Transforming Growth Factor beta1/genetics; Transforming Growth Factor beta1/metabolism*; Transforming Growth Factor beta2/genetics; Transforming Growth Factor beta2/metabolism*; Epithelial-Mesenchymal Transition; Cancer-Associated Fibroblasts/metabolism; Cancer-Associated Fibroblasts/pathology*
  16. Jahromi MF, Liang JB, Ebrahimi R, Soleimani AF, Rezaeizadeh A, Abdullah N, et al.
    Animal, 2017 May;11(5):755-761.
    PMID: 27804905 DOI: 10.1017/S175173111600224X
    To alleviate adverse effects of heavy metal toxicity, diverse range of removing methods have been suggested, that is usage of algae, agricultural by-products and microorganisms. Here, we investigated lead (Pb) biosorption efficacy by two lactic acid bacteria species (LABs) in broiler chickens. In an in vitro study, Pb was added to culture medium of LABs (Lactobacillus pentosus ITA23 and Lactobacillus acidipiscis ITA44) in the form of lead acetate. Results showed that these LABs were able to absorb more than 90% of Pb from the culture medium. In follow-up in vivo study, LABs mixture was added to diet of broiler chickens contained lead acetate (200 mg/kg). Pb exposure significantly increased lipid peroxidation and decreased antioxidant activity in liver. The changes were recovered back to normal level upon LABs supplementation. Moreover, addition of LABs eliminated the liver tissue lesion and the suppressed performance in Pb-exposed chicks. Analysis of liver and serum samples indicated 48% and 28% reduction in Pb accumulation, respectively. In conclusion, results of this study showed that L. pentosus ITA23 and L. acidipiscis ITA44 effectively biosorb and expel dietary Pb from gastrointestinal tract of chickens.
    MeSH terms: Animals; Animal Feed/analysis; Chickens/metabolism*; Diet/veterinary; Environmental Pollutants/metabolism*; Lactobacillus/chemistry*; Lead/metabolism*; Male; Random Allocation; Silage/microbiology; Probiotics/administration & dosage; Probiotics/pharmacology*; Morus/microbiology
  17. Saleh A, Kong YH, Haron N, Aripin SF, Vadiveloo M, Hussaini H, et al.
    Community Dent Oral Epidemiol, 2017 04;45(2):112-119.
    PMID: 27805279 DOI: 10.1111/cdoe.12266
    OBJECTIVES: Private dental practitioners constitute approximately 40% of all registered dentists in Malaysia, and this group affords an avenue for prevention and early detection of oral cancer. However, such activities are still limited. This study investigated the feasibility of incorporating opportunistic screening of oral cancer in the private dental setting.

    METHODS: Dentists were recruited through two main dental associations in Malaysia and attended a 1-day training session on recognizing abnormalities within the oral cavity. Following the training, the dentists conducted screening and provided risk habits cessation advice at their respective clinics for 6 months. The impact of the program was evaluated by determining the number of patients who were screened and/or provided with risk habits cessation advice.

    RESULTS: Twenty-six dentists took part in the program and conducted opportunistic screening on a total of 2603 individuals. On average, they screened about 23.0% of their patients and 5.1% were given risk habits cessation advice. Notably, dentists who had lower patient load were more likely to conduct opportunistic screening.

    CONCLUSIONS: While the participating private dentists state that they have a role in performing opportunistic screening and providing risk habits cessation advice, these activities are still not a priority area in the private clinics, strongly suggesting that strategies to motivate dentists in this setting are urgently needed.

    MeSH terms: Adult; Developing Countries; Education, Dental, Continuing; Female; Humans; Malaysia; Male; Mass Screening/statistics & numerical data*; Middle Aged; Mouth Neoplasms/diagnosis*; Risk Assessment; Practice Patterns, Dentists'/statistics & numerical data*; Early Detection of Cancer*
  18. Sha'ari N, Manaf ZA, Ahmad M, Rahman FN
    Pediatr Int, 2017 Apr;59(4):408-415.
    PMID: 27805287 DOI: 10.1111/ped.13196
    BACKGROUND: Children with attention deficit-hyperactivity disorder (ADHD) may be at risk of nutrient deficiency due to the inability to sit through meals. This comparative cross-sectional study was therefore carried out to determine the nutritional status and feeding problems of ADHD children aged 4-12 years.

    METHODS: Sociodemographic data, anthropometric measurements and 3 day dietary intake record were collected from 54 ADHD children and 54 typical development (TD) children. The Behavioral Pediatrics Feeding Assessment Scale was used to assess feeding problems.

    RESULTS: Mean subject age was 8.6 ± 2.1 years. On anthropometric assessment, 11.1% of the ADHD children had wasting, while 1.9% had severe wasting. In contrast, none of the TD children had wasting. Approximately 5.6% of the ADHD children had stunting, as compared with 3.7% of the TD children, while none of the TD children had severe stunting compared with 3.7% of the ADHD children. More than half of the ADHD children had mid-upper arm circumference (MUAC) below the 5th percentile, indicating undernutrition, compared with only 35.2% of TD children. More than one-third of the ADHD children had feeding problems compared with 9.3% of TD children. There was a significant negative relationship between the ADHD children's feeding problems and bodyweight (r = -0338, P = 0.012), body mass index (r = -0322, P = 0.017) and MUAC (r = -0384, P = 0.004).

    CONCLUSION: Almost half of the ADHD children had suboptimal nutrition compared with 11.1% of the TD children. It is imperative to screen ADHD children for nutritional status and feeding problems to prevent negative health impacts later on.

    MeSH terms: Attention Deficit Disorder with Hyperactivity/complications*; Attention Deficit Disorder with Hyperactivity/physiopathology; Child; Child, Preschool; Cross-Sectional Studies; Diet Surveys; Female; Humans; Male; Nutritional Status*; Case-Control Studies; Feeding and Eating Disorders of Childhood/diagnosis; Feeding and Eating Disorders of Childhood/etiology*; Feeding and Eating Disorders of Childhood/epidemiology; Malnutrition/diagnosis; Malnutrition/etiology*; Malnutrition/epidemiology
  19. Uncini A, Shahrizaila N, Kuwabara S
    J Neurol Neurosurg Psychiatry, 2017 03;88(3):266-271.
    PMID: 27799296 DOI: 10.1136/jnnp-2016-314310
    In 2016, we have seen a rapid emergence of Zika virus-associated Guillain-Barré syndrome (GBS) since its first description in a French-Polynesian patient in 2014. Current evidence estimates the incidence of GBS at 24 cases per 100 000 persons infected by Zika virus. This will result in a sharp rise in the number of GBS cases worldwide with the anticipated global spread of Zika virus. A better understanding of the pathogenesis of Zika-associated GBS is crucial to prepare us for the current epidemic. In this review, we evaluate the existing literature on GBS in association with Zika and other flavivirus to better define its clinical subtypes and electrophysiological characteristics, demonstrating a demyelinating subtype of GBS in most cases. We also recommend measures that will help reduce the gaps in knowledge that currently exist.
    MeSH terms: Zika Virus Infection/epidemiology; Zika Virus/isolation & purification; Disease Outbreaks*; Humans; Incidence; Guillain-Barre Syndrome/classification; Guillain-Barre Syndrome/epidemiology; Guillain-Barre Syndrome/physiopathology*; Electrophysiological Phenomena*
  20. Beau De Rochars VM, Lednicky J, White S, Loeb J, Elbadry MA, Telisma T, et al.
    Am J Trop Med Hyg, 2017 Jan 11;96(1):144-147.
    PMID: 27799635 DOI: 10.4269/ajtmh.16-0585
    Human coronavirus (HCoV) NL63 is recognized as a common cause of upper respiratory infections and influenza-like illness. In screening children with acute undifferentiated febrile illness in a school cohort in rural Haiti, we identified HCoV-NL63 in blood samples from four children. Cases clustered over an 11-day period; children did not have respiratory symptoms, but two had gastrointestinal complaints. On phylogenetic analysis, the Haitian HCoV-NL63 strains cluster together in a highly supported monophyletic clade linked most closely with recently reported strains from Malaysia; two respiratory HCoV-NL63 strains identified in north Florida in the same general period form a separate clade, albeit again with close linkages with the Malaysian strains. Our data highlight the variety of presentations that may be seen with HCoV-NL63, and underscore the apparent ease with which CoV strains move among countries, with our data consistent with recurrent introduction of strains into the Caribbean (Haiti and Florida) from Asia.
    MeSH terms: Asia; Child; Florida; Haiti; Humans; Influenza, Human; Malaysia; Phylogeny; Respiratory Tract Infections; Cohort Studies; Caribbean Region; Coronavirus NL63, Human
External Links