Browse publications by year: 2019

  1. Bayat M, Alarifi IM, Khalili AA, El-Bagory TMAA, Nguyen HM, Asadi A
    Sci Rep, 2019 Oct 25;9(1):15317.
    PMID: 31653877 DOI: 10.1038/s41598-019-51450-z
    A thermo-elastic contact problem of functionally graded materials (FGMs) rotating brake disk with different pure brake pad areas under temperature dependent material properties is solved by Finite Element Method (FEM). The properties of brake disk change gradually from metal to ceramic by power-law distribution along the radial direction from the inner to the outer surface. Areas of the pure pad are changing while the vertical force is constant. The ratio of brake pad thickness to FGMs brake disk thickness is assumed 0.66. Two sources of thermal loads are considered: (1) Heat generation between the pad and brake disk due to contact friction, and (2) External thermal load due to a constant temperature at inner and outer surfaces. Mechanical responses of FGMs disk are compared with several pad contact areas. The results for temperature-dependent and temperature-independent material properties are investigated and presented. The results show that the absolute value of the shear stress in temperature-dependent material can be greater than that for temperature-independent material. The radial stress for some specific grading index (n = 1.5) is compressive near the inner surface for double contact while it is tensile for a single contact. It is concluded that the radial strain for some specific value of grading index (n = 1) is lower than other FGMs and pure double side contact brake disks.
    MeSH terms: Ceramics; Hot Temperature; Metals; Pressure; Stress, Mechanical; Temperature; Friction; Finite Element Analysis; Mechanical Phenomena
  2. Todd J, Aspell JE, Barron D, Swami V
    Body Image, 2019 Dec;31:171-180.
    PMID: 31654981 DOI: 10.1016/j.bodyim.2019.10.004
    Previous research suggests that lower interoceptive awareness is associated with more negative body image during adolescence. However, studies have not distinguished between adaptive and maladaptive modes of attention toward interoceptive signals, and relationships between interoceptive awareness and positive body image remain unexplored. To address these issues, a sample of 265 British adolescents (140 girls, 125 boys) aged 13-16 years completed measures of interoceptive awareness, body appreciation, functionality appreciation, body pride, body shame, and body surveillance. Correlational analyses broadly indicated that greater interoceptive awareness was significantly associated with more positive body image. Multiple regressions revealed significant predictive relationships between interoceptive awareness and all facets of body image in both girls and boys, except body surveillance, which was not statistically significant for girls. At the univariate level, the interoceptive awareness facets of Attention Regulation, Body Listening, Self-Regulation, and Trusting emerged as significant predictors for at least one facet of positive body image, whilst the Noticing and Emotional Awareness facets did not. These findings broadly align with previous research with adults, which has indicated that the way interoceptive stimuli are appraised and responded to might be more closely associated with facets of body image than the tendency to notice interoceptive stimuli.
    MeSH terms: Adolescent; Adult; Awareness/physiology*; Body Image/psychology*; Female; Humans; Male; Interoception/physiology*
  3. Azizi P, Osman M, Hanafi MM, Sahebi M, Yusop MR, Taheri S
    Plant Physiol Biochem, 2019 Nov;144:466-479.
    PMID: 31655345 DOI: 10.1016/j.plaphy.2019.10.014
    Pyricularia oryzae (P. oryzae), one of the most devastating fungal pathogens, is the cause of blast disease in rice. Infection with a blast fungus induces biological responses in the host plant that lead to its survival through the termination or suppression of pathogen growth, and metabolite compounds play vital roles in plant interactions with a wide variety of other organisms. Numerous studies have indicated that rice has a multi-layered plant immune system that includes pre-developed (e.g., cell wall and phytoanticipins), constitutive and inducible (phytoalexins) defence barriers against stresses. Significant progress towards understanding the basis of the molecular mechanisms underlying the defence responses of rice to P. oryzae has been achieved. Nonetheless, even though the important metabolites in the responses of rice to pathogens have been identified, their exact mechanisms and their contributions to plant immunity against blast fungi have not been elucidated. The purpose of this review is to summarize and discuss recent advances towards the understanding of the integrated metabolite variations in rice after P. oryzae invasion.
    MeSH terms: Adaptation, Physiological; Plant Diseases/microbiology; Oryza/metabolism*; Oryza/microbiology*; Magnaporthe/pathogenicity; Host-Pathogen Interactions; Plant Immunity
  4. Hizam NDA, Ung NM, Jong WL, Zin HM, Rahman ATA, Loh JPY, et al.
    Phys Med, 2019 Nov;67:34-39.
    PMID: 31655398 DOI: 10.1016/j.ejmp.2019.10.023
    PURPOSE: Intensity Modulated Radiotherapy (IMRT) has changed the practice of radiotherapy since its implementation in the 1990s. The purpose of this study is to review current practice of IMRT in Malaysia.

    METHODS: A survey on medical physics aspects of IMRT is conducted on radiotherapy departments across Malaysia to assess the usage, experience and QA in IMRT, which is done for the first time in this country. A set of questionnaires was designed and sent to the physicist in charge for their responses. The questionnaire consisted of four sections; (i) Experience and qualification of medical physicists, (ii) CT simulation techniques (iii) Treatment planning and treatment unit, (iv) IMRT process, delivery and QA procedure.

    RESULTS: A total of 26 responses were collected, representing 26 departments out of 33 radiotherapy departments in operation across Malaysia (79% response rate). Results showed that the medical physics aspects of IMRT practice in Malaysia are homogenous, with some variations in certain areas of practices. Thirteen centres (52%) performed measurement-based QA using 2D array detector and analysed using gamma index criteria of 3%, 3 mm with variation confidence range. In relation to the IMRT delivery, 44% of Malaysia's physicist takes more than 8 h to plan a head and neck case compared to the UK study possibly due to the lack of professional training.

    CONCLUSIONS: This survey provides a picture of medical physics aspects of IMRT in Malaysia where the results/data can be used by radiotherapy departments to benchmark their local policies and practice.

    MeSH terms: Clinical Competence/statistics & numerical data; Humans; Malaysia; Physics*; Quality Control; Radiotherapy Planning, Computer-Assisted; Tomography, X-Ray Computed; Radiotherapy, Intensity-Modulated*
  5. Fang F, Chang Q, Sheng Z, Zhang Y, Yin Z, Guillot J
    Parasitol Res, 2019 Dec;118(12):3237-3240.
    PMID: 31655903 DOI: 10.1007/s00436-019-06464-x
    Chrysomya bezziana is an obligate, myiasis-causing fly in humans and warm-blooded animals throughout the tropical and subtropical Old World. We report a case of cutaneous myiasis due to C. bezziana in a dog from Guangxi province in China. A total of 35 maggots were removed from the lesions. Direct sequencing of the mitochondrial cytochrome b gene showed that the specimen belonged to haplotype CB_bezz02, which was previously reported in Malaysia and the Gulf region. This paper also reviews reported cases of screwworm myiasis from humans and animals in China. Geographical records indicate that the distribution of C. bezziana is expanding, suggesting that an integrated pest management control should be taken into consideration in China.
    MeSH terms: Animals; Animals, Domestic; China; Diptera; Dogs; Haplotypes; Humans; Larva; Malaysia; Myiasis; Pest Control; Screw Worm Infection; Cytochromes b
  6. Koo HC, Hadirah Z, Airina A, Nurul Alifatul Amrina R, Faziela N
    Afr Health Sci, 2019 Jun;19(2):2243-2251.
    PMID: 31656510 DOI: 10.4314/ahs.v19i2.50
    BACKGROUND: Dietary composition is the cornerstone of weight management. This study aimed to investigate the effect of nutrient intakes on anthropometric profiles among university students.

    METHODS: This cross-sectional study was conducted among 411 students aged 18-29 years, purposive sampled from a selected private university in Klang Valley, Malaysia. Anthropometric profiles were measured. Nutrient intakes were assessed by 3-day 24-hour diet recalls.

    RESULTS: Respondents on average had adequate macronutrient intakes, however, total consumption of dietary fiber and micronutrients were fell short of recommended levels. Significant negative associations were found between body mass index (BMI) and all the macronutrients, calcium, thiamine, riboflavin and niacin. Body fat percentage was significantly associated with all the macronutrients, calcium, zinc, thiamine and niacin. Significant inverse associations were also found between waist circumference and carbohydrate, fiber, thiamine, riboflavin and niacin. Visceral fat showed significant inverse associations with carbohydrate, fat, fiber, thiamine, riboflavin and niacin. Further, after adjusting for sex, gender and race, BMI was associated with niacin (β=-0.161, p=0.027). Body fat percentage was also found significantly associated with niacin (β=-0.180, p=0.002) and riboflavin (β=-0.132, p=0.014).

    CONCLUSION: Micronutrients, especially B vitamins, are important in weight management among the young adults.

    MeSH terms: Adolescent; Adult; Anthropometry*; Body Weight; Energy Intake*; Cross-Sectional Studies; Female; Humans; Malaysia; Male; Surveys and Questionnaires; Universities; Diet Records
  7. Poudel A, Kc B, Shrestha S, Nissen L
    J Glob Health, 2019 12;9(2):020309.
    PMID: 31656599 DOI: 10.7189/jogh.09.020309
    MeSH terms: Developing Countries*; Humans; Palliative Care*; Developed Countries*; Healthcare Disparities/statistics & numerical data*
  8. Abdul Rahman SN, Bakar MFA, Singham GV, Othman AS
    3 Biotech, 2019 Nov;9(11):388.
    PMID: 31656726 DOI: 10.1007/s13205-019-1921-3
    In this study, RNA sequencing of several Hevea brasiliensis clones grown in Malaysia with different annual rubber production yields and disease resistance was performed on the Illumina platform. A total of 29,862,548 reads were generated, resulting in 101,269 assembled transcripts that were used as the reference transcripts. A similarity search against the non-redundant (nr) protein databases presented 83,771 (83%) positive BLASTx hits. The transcriptome was annotated using gene ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Pfam database. A search for putative molecular markers was performed to identify single-nucleotide polymorphisms (SNPs). Overall, 3,210,629 SNPs were detected and a total of 1314 SNPs associated with the genes involved in MVA and MEP pathways were identified. A total of 176 SNP primer pairs were designed from sequences that were related to the MVA and MEP pathways. The transcriptome of RRIM 3001 and RRIM 712 were subjected to pairwise comparison and the results revealed that there were 1262 significantly differentially expressed genes unique to RRIM 3001, 1499 significantly differentially expressed genes unique to RRIM 712 and several genes related to the MVA and MEP pathways such as AACT, HMGS, PMK, MVD, DXS and HDS were included. The results will facilitate the characterization of H. brasiliensis transcriptomes and the development of a new set of molecular markers in the form of SNPs from transcriptome assembly for the genotype identification of various rubber varieties with superior traits in Malaysia.
    MeSH terms: Base Sequence; Genotype; Malaysia; Nucleotides; Rubber; Sequence Analysis, RNA; Polymorphism, Single Nucleotide; Hevea; Databases, Protein; Transcriptome; Disease Resistance; Gene Ontology
  9. Rusdi JF, Salam S, Abu NA, Sunaryo B, Taufiq R, Muchlis LS, et al.
    Data Brief, 2019 Dec;27:104610.
    PMID: 31656841 DOI: 10.1016/j.dib.2019.104610
    This article contains dataset on the behavior of international tourists when traveling is related to 1) tourist demographics, 2) things that affect tourists to choose travel destinations when planning, 3) use of mobile data while traveling, 4) how to get internet access while traveling, 5) social media used during traveling, and 6) behavior of smartphone use for tourists during traveling. The raw data presented here can be used as material to analyze the behavior of international tourists related to any media that affects international tourists in planning their trips, and how they behave during traveling. This data is a source of raw data from our research on smartphones and international tourist behavior, besides being used for various other research purposes.
  10. Sung TC, Liu CH, Huang WL, Lee YC, Kumar SS, Chang Y, et al.
    Biomater Sci, 2019 Oct 28.
    PMID: 31656967 DOI: 10.1039/c9bm00817a
    Current xeno-free and chemically defined methods for the differentiation of hPSCs (human pluripotent stem cells) into cardiomyocytes are not efficient and are sometimes not reproducible. Therefore, it is necessary to develop reliable and efficient methods for the differentiation of hPSCs into cardiomyocytes for future use in cardiovascular research related to drug discovery, cardiotoxicity screening, and disease modeling. We evaluated two representative differentiation methods that were reported previously, and we further developed original, more efficient methods for the differentiation of hPSCs into cardiomyocytes under xeno-free, chemically defined conditions. The developed protocol successively differentiated hPSCs into cardiomyocytes, approximately 90-97% of which expressed the cardiac marker cTnT, with beating speeds and sarcomere lengths that were similar to those of a healthy adult human heart. The optimal cell culture biomaterials for the cardiac differentiation of hPSCs were also evaluated using extracellular matrix-mimetic material-coated dishes. Synthemax II-coated and Laminin-521-coated dishes were found to be the most effective and efficient biomaterials for the cardiac differentiation of hPSCs according to the observation of hPSC-derived cardiomyocytes with high survival ratios, high beating colony numbers, a similar beating frequency to that of a healthy adult human heart, high purity levels (high cTnT expression) and longer sarcomere lengths similar to those of a healthy adult human heart.
    MeSH terms: Biocompatible Materials; Cell Differentiation; Extracellular Matrix; Humans; Laminin; Sarcomeres; Cell Culture Techniques; Myocytes, Cardiac; Pluripotent Stem Cells; Drug Discovery; Cardiotoxicity
  11. Si LY, Ramalingam A, Ali SS, Aminuddin A, Ng PY, Latip J, et al.
    EXCLI J, 2019;18:876-892.
    PMID: 31645847 DOI: 10.17179/excli2019-1792
    Roselle (Hibiscus sabdariffa Linn) has been traditionally used as folk medicine for hypertension and maintaining cardiovascular health, with therapeutic potential in protecting against numerous cardiovascular diseases. However, it remains unclear whether roselle can be used for management of cardiac hypertrophy seen after myocardial infarction (MI). This study therefore investigated the effects of aqueous roselle extract on cardiac hypertrophy arising from myocardial infarction both in vivo and in vitro. For in vivo study, male Sprague-Dawley rats were divided into control or MI groups (receiving 85 mg/kg isoproterenol s.c. for 2 days) and were given roselle extract (100 mg/kg, p.o daily) for 28 days. Cardiac structure and functional changes were evaluated at study end-point using histology, Langendorff analysis and gene expression analysis. In vitro effects of roselle were also assessed on ANG II-induced cardiomyocytes hypertrophy using H9c2 cells, simulating cardiac hypertrophy evident after MI. Roselle significantly ameliorated MI-induced cardiac systolic and diastolic dysfunction, as seen across improvement in left ventricular developed pressure (LVDP) and its derivative (LVdP/dtmax) and isovolumic relaxation (Tau). Oxidative stress evident across elevated pro-oxidant markers (NOX2 subunit of NADPH oxidase and 8-isoprostane) as well as reduced antioxidant markers (superoxide dismutase and glutathione) were also significantly attenuated by roselle. Furthermore, roselle treatment markedly reduced markers of cardiac remodeling (cardiac hypertrophy and fibrosis) compared to the untreated MI rats. On in vitro analysis, roselle significantly attenuated ANG II-induced cardiomyoycte hypertrophy in dose-dependent manner. This study demonstrated that roselle attenuates cardiac hypertrophy and dysfunction seen after MI both in vivo and in vitro, and these effects are likely mediated by phenolic compounds found in roselle extract.
  12. Teh JKL, Tey NP
    SSM Popul Health, 2019 Dec;9:100479.
    PMID: 31646167 DOI: 10.1016/j.ssmph.2019.100479
    This study examined three types of leisure activities (playing cards/mahjong, watching TV/listening to the radio, and participation in social activities) among the older segment of the Chinese population, and the effects of these activities in preventing the feelings of loneliness. Data came from the Chinese Longitudinal Healthy Longevity Survey (CLHLS), conducted in 2005, 2008, and 2011. The males, those who were educated, the young-old (aged between 65 and 74 years), urban dwellers, and living in institutions were more likely to participate in all three activities frequently. Frequent or occasional participation in these three activities was negatively associated with feelings of loneliness. The longitudinal study from 2005 to 2011 showed that respondents who frequently played cards/mahjong at baseline were less likely to feel persistent loneliness at the end of the 2011 wave. Instead, frequently watching TV/listening to the radio in 2008 was associated with lower odds in feeling persistent loneliness at the end of 2011. Hence, active participation in playing cards/mahjong and TV/radio entertainment can be effective treatment intervention against persistent loneliness among older Chinese. For the Chinese, a leisure activity steeped in a nation's culture and heritage, such as playing mahjong, may be used as an intervention strategy to prevent and alleviate chronic loneliness among older adults.
  13. Goh EW, Heidelberg T, Duali Hussen RS, Salman AA
    ACS Omega, 2019 Oct 15;4(16):17039-17047.
    PMID: 31646251 DOI: 10.1021/acsomega.9b02809
    Aiming for glycolipid-based vesicles for targeted drug delivery, cationic Guerbet glycosides with spacered click functionality were designed and synthesized. The cationic charge promoted the distribution of the glycolipids during the formulation, thereby leading to homogeneously small vesicles. The positive surface charge of the vesicles stabilizes them against unwanted fusion and promotes interactions of the drug carriers with typical negative charge-dominated target cells. High bioconjugation potential of the functionalized glycolipids based on the copper-catalyzed azide alkyne cycloaddition makes them highly valuable components for targeted drug delivery systems.
    MeSH terms: Alkynes; Azides; Catalysis; Cations; Copper; Drug Carriers; Glycolipids; Glycosides; Drug Delivery Systems; Cycloaddition Reaction
  14. Huang CB, Xiao L, Xing SC, Chen JY, Yang YW, Zhou Y, et al.
    BMC Genomics, 2019 Oct 23;20(1):770.
    PMID: 31646963 DOI: 10.1186/s12864-019-6115-1
    BACKGROUND: Host genotype plays a crucial role in microbial composition of laying hens, which may lead to dissimilar odor gas production. The objective of this study was to investigate the relationship among layer breed, microbial structure and odor production.

    RESULTS: Thirty Hy-Line Gray and thirty Lohmann Pink laying hens were used in this study to determine the impact of cecal microbial structure on odor production of laying hens. The hens were managed under the same husbandry and dietary regimes. Results of in vivo experiments showed a lower hydrogen sulfide (H2S) production from Hy-Line hens and a lower concentration of soluble sulfide (S2-) but a higher concentration of butyrate in the cecal content of the Hy-Line hens compared to Lohmann Pink hens (P  0.05). Significant microbial structural differences existed between the two breed groups. The relative abundance of some butyrate producers (including Butyricicoccus, Butyricimonas and Roseburia) and sulfate-reducing bacteria (including Mailhella and Lawsonia) were found to be significantly correlated with odor production and were shown to be different in the 16S rRNA and PCR data between two breed groups. Furthermore, some bacterial metabolism pathways associated with energy extraction and carbohydrate utilization (oxidative phosphorylation, pyruvate metabolism, energy metabolism, two component system and secretion system) were overrepresented in the Hy-Line hens, while several amino acid metabolism-associated pathways (amino acid related enzymes, arginine and proline metabolism, and alanine-aspartate and glutamate metabolism) were more prevalent in the Lohmann hens.

    CONCLUSION: The results of this study suggest that genotype of laying hens influence cecal microbiota, which in turn modulates their odor production. Our study provides references for breeding and enteric manipulation for defined microbiota to reduce odor gas emission.

    MeSH terms: Gastrointestinal Microbiome*; Animals; Bacteria/classification*; Bacteria/metabolism; Butyrates; Cecum/microbiology*; Chickens/microbiology*; Female; Hydrogen Sulfide/metabolism*; Odors; RNA, Ribosomal, 16S/genetics
  15. Brennan M, Paterson L, Baharudin AAA, Stanisz-Migal M, Hoebe PN
    J Plant Physiol, 2019 Dec;243:153054.
    PMID: 31648109 DOI: 10.1016/j.jplph.2019.153054
    Adhesion of the barley husk to the underlying caryopsis requires the development of a cuticular cementing layer on the caryopsis surface. Differences in adhesion quality among genotypes have previously been correlated with cementing layer composition, which is thought to influence caryopsis cuticle permeability, the hypothesised mechanism of adhesion mediation. It is not yet known whether differences in adhesion quality among genotypes are determined by changes in caryopsis cuticle permeability. We examined changes in candidate cementing layer biosynthetic and regulatory genes to investigate the genetic mechanisms behind husk adhesion quality. We used both commercially relevant UK malting cultivars and older European lines to ensure phenotypic diversity in adhesion quality. An ethylene responsive transcription factor (NUD) is required for the development of the cementing layer. To examine correlations between gene expression, cementing layer permeability and husk adhesion quality we also treated cultivars with ethephon (2-chloroethylphosphonic acid) which breaks down to ethylene, and silver thiosulphate which inhibits ethylene reception, and measured caryopsis cuticle permeability. Differential adhesion qualities among genotypes are not determined by NUD expression during development of the cementing material alone, but could result from differences in biosynthetic gene expression during cementing layer development in response to longer-term NUD expression patterns. Altered caryopsis cuticle permeability does result in altered adhesion quality, but the correlation is not consistently positive or negative. Cuticle permeability is therefore not the mechanism that determines husk adhesion quality, but is likely a consequence of the required cuticular compositional changes that determine adhesion.
    MeSH terms: Adhesiveness; Hordeum/genetics; Hordeum/physiology*; Ethylenes/antagonists & inhibitors; Ethylenes/metabolism*; Organophosphorus Compounds/pharmacology*; Permeability; Plant Growth Regulators/pharmacology*; Plant Proteins/genetics; Plant Proteins/metabolism; Seeds/genetics; Seeds/physiology*; Thiosulfates/pharmacology*; Transcription Factors/genetics; Transcription Factors/metabolism; Gene Expression/physiology
  16. Dharmani M, Kamarulzaman K, Giribabu N, Choy KW, Zuhaida MZ, Aladdin NA, et al.
    Phytomedicine, 2019 Dec;65:153101.
    PMID: 31648126 DOI: 10.1016/j.phymed.2019.153101
    BACKGROUND: Oestrogen deficiency leads to metabolic disturbances such as insulin resistance and impairment of adipose tissue or lipid metabolism. Marantodes pumilum (Blume) Kuntze (Primulaceae) is believed to have phytoestrogenic properties and is claimed to have beneficial effects in the treatment of diabetes mellitus (DM), but the mechanism behind its phytoestrogenic effects on estrogen-deficient diabetic condition have not been fully examined.

    PURPOSE: The present study investigated the effects of oral treatment with M. pumilum var. alata (MPA) extracts on the estrogen receptor, metabolic characteristics and insulin signaling pathway in pancreas and liver of ovariectomised nicotidamide streptozotocin-induced diabetes in female rats.

    MATERIALS AND METHODS: Ovariectomised diabetic (OVXS) Sprague-Dawley rats were orally administered with either aqueous leaf extract and ethanol (50%) stem-root extract of MPA (50 or 100 mg/kg) respectively for 28 days. Metabolic parameters were evaluated by measuring fasting blood glucose, serum insulin, oral glucose and insulin tolerance test. Distribution and expression level of insulin, oxidative stress and inflammatory marker in the pancreatic islets and liver were evaluated by immunohistochemistry and western blot, respectively.

    RESULTS: Oral treatment with aqueous leaf and ethanol (50%) stem-root extracts of MPA (100 mg/kg) significantly reversed the elevated fasting blood glucose, impaired glucose and insulin tolerance. The protein expression of insulin, glucose transporter (GLUT-2 and GLUT-4) increased in the pancreatic islets and liver. Furthermore, marked improvement in the tissue morphology following treatment with MPA was observed. Similarly, the western blots analysis denotes improved insulin signaling in the liver and decreased reactive oxygen species producing enzymes, inflammatory and pro-apoptotic molecules with MPA treatment.

    CONCLUSIONS: Taken together, this work demonstrate that 100 mg/kg of aqueous leaf extract and ethanol (50%) stem-root extract of MPA improves β-cell function and insulin signaling in postmenopausal diabetes through attenuation of oxidative stress and partially mediated by oestrogen receptor stimulation.

    MeSH terms: Animals; Blood Glucose/metabolism; Diabetes Mellitus, Experimental/drug therapy; Diabetes Mellitus, Experimental/prevention & control*; Female; Insulin/metabolism*; Liver/drug effects; Liver/metabolism; Pancreas/drug effects; Pancreas/metabolism; Plant Extracts/administration & dosage; Plant Extracts/pharmacology*; Receptors, Estrogen/metabolism; Signal Transduction/drug effects; Rats, Sprague-Dawley; Postmenopause; Oxidative Stress/drug effects; Primulaceae/chemistry*; Insulin-Secreting Cells/drug effects*; Insulin-Secreting Cells/physiology; Glucose Transporter Type 2/metabolism; Glucose Transporter Type 4/metabolism
  17. Fui LW, Lok MPW, Govindasamy V, Yong TK, Lek TK, Das AK
    J Tissue Eng Regen Med, 2019 12;13(12):2218-2233.
    PMID: 31648415 DOI: 10.1002/term.2966
    Mesenchymal stem cells (MSCs) transplantation seems to be a promising new therapy for diabetic wound healing (DWH), and currently, arrays of MSCs from various sources ranging from umbilical, adipose to dental sources are available as a treatment modality for this disease. However, it now appears that only a fraction of transplanted cells actually assimilate and survive in host tissues suggesting that the major mechanism by which stem cells participate in tissue repair are most likely related to their secretome level. These include a wide range of growth factors, cytokines, and chemokines, which can be found from the conditioned medium (CM) used to culture the cells. Basic studies and preclinical work confirm that the therapeutic effect of CMs are comparable with the application of stem cells. This review describes in detail the wound healing process in diabetes and the cellular and biological factors that influence the process. Subsequently, through a comprehensive literature search of studies related to wound healing in diabetics, we aim to provide an overview of scientific merits of using MSCs-CM in the treatment of diabetic wound as well as the significant caveats, which restricts its potential use in clinical set-ups. To our best knowledge, this is one of the first review papers that collect the importance of stem cells as an alternative treatment to the DWH. We anticipate that the success of this treatment will have a significant clinical impact on diabetic wounds.
    MeSH terms: Animals; Humans; Wound Healing/drug effects*; Wounds and Injuries/drug therapy*; Wounds and Injuries/metabolism; Wounds and Injuries/pathology; Culture Media, Conditioned/metabolism; Culture Media, Conditioned/pharmacology; Diabetes Complications/drug therapy*; Diabetes Complications/metabolism; Diabetes Complications/pathology; Mesenchymal Stromal Cells/metabolism*
  18. Che Salleh N, Mohamad Anuar MF, Abdullah NA, Yaw SL, Ibrahim Wong N, Teck Pei T, et al.
    Asia Pac J Public Health, 2019 11;31(8_suppl):97S-104S.
    PMID: 31640393 DOI: 10.1177/1010539519880998
    This cross-sectional national survey aimed to determine the prevalence of oral and hand hygiene practices as well as associations between sociodemographic characteristics and oral and hygiene practice among adolescents aged 13 to 17 years during the past 30 days. A validated self-administered bilingual questionnaire adapted from the Malaysian Global School Health Survey with computer-scanable answer sheets was distributed to 30 823 adolescents attending government secondary schools in Malaysia; and 89.2% responded to the questionnaire given. More than 80% (87.1%; 95% confidence interval [CI] = 86.24-87.96) of the adolescents reported that they had brushed their teeth at least twice daily and had always washed their hands after using the toilet (86.6%; 95% CI = 85.85-87.36). The odds of adolescents who brushed their teeth at least 2 times daily and washed their hands after using the toilet was significantly associated with sociodemographic characteristics. This study highlights the need to further improve the effectiveness of current oral health promotion programs with consideration for sociodemographic characteristics.
  19. Zhang C, Gao Y, Ning Z, Lu Y, Zhang X, Liu J, et al.
    Genome Biol, 2019 10 22;20(1):215.
    PMID: 31640808 DOI: 10.1186/s13059-019-1838-5
    Despite the tremendous growth of the DNA sequencing data in the last decade, our understanding of the human genome is still in its infancy. To understand the implications of genetic variants in the light of population genetics and molecular evolution, we developed a database, PGG.SNV ( https://www.pggsnv.org ), which gives much higher weight to previously under-investigated indigenous populations in Asia. PGG.SNV archives 265 million SNVs across 220,147 present-day genomes and 1018 ancient genomes, including 1009 newly sequenced genomes, representing 977 global populations. Moreover, estimation of population genetic diversity and evolutionary parameters is available in PGG.SNV, a unique feature compared with other databases.
    MeSH terms: Disease/genetics*; Gene Frequency; Humans; Genetic Variation; Genome, Human*; Evolution, Molecular; Polymorphism, Single Nucleotide*; Databases, Genetic*; Asian Continental Ancestry Group/genetics*
External Links