Browse publications by year: 2019

  1. Mahdavi Gorabi A, Hasani M, Djalalinia S, Zarei M, Ejtahed H, Abdar ME, et al.
    J Diabetes Metab Disord, 2019 Dec;18(2):349-362.
    PMID: 31890660 DOI: 10.1007/s40200-019-00419-w
    PURPOSE: The association between selenium supplementation and glycemic indices seems to be a controversial issue. This systematic review and meta-analysis was conducted to evaluate the effect of selenium supplementation on glycemic indices.

    METHODS: We systematically searched PubMed/MEDLINE, ISI/WOS, and Scopus (from their commencements up to Jan 2016) for relevant studies examining the association between intake of selenium and glycemic indices. The data were extracted from relevant qualified studies and estimated using the random-effect or pooled model and standardized mean difference (SMD) with 95% confidence interval (CI).

    RESULTS: Twelve articles published between 2004 and 2016 were included. In all the studies, the participants were randomly assigned to an intervention group (n = 757) or a control group(n = 684). All the studies were double blind, placebo controlled trials. Selenium supplementation resulted in a significant decrease in homeostasis model of assessment-estimated β-cell function (HOMA-B) (SMD: -0.63; 95%CI: -0.89 to -0.38) and a significant increase in quantitative insulin sensitivity check index (QUICKI) (SMD: by 0.74; 95%CI: 0.49 to 0.1) as compared with the controls. There were no statistically significant improvements in glycemic indices, such as fasting plasma glucose (FPG), insulin, homeostasis model of assessment-estimated insulin resistance (HOMA-IR), Hemoglobin A1c (HbA1c) and adiponectin.

    CONCLUSION: This meta-analysis indicated that selenium supplementation significantly decreased HOMA-B and increased QUICKI score. There was no statistically significant improvement in FPG, insulin, HOMA-IR, HbA1c and adiponectin indices following selenium supplementation.

  2. Taufik N, Hanafiah MH
    Heliyon, 2019 Dec;5(12):e02960.
    PMID: 31890945 DOI: 10.1016/j.heliyon.2019.e02960
    Purpose: The purpose of this paper is to examine the factors influencing passenger adoption and behaviour of self-service technology (SST) in airports. This study adopted the Theory Acceptance Model (TAM) and extended the model by including the need for human interaction (NI) construct in the study framework.

    Design/methodology/approach: The research framework is based on the theoretical concepts of SST usage from the inter-disciplinary field. Four hundred two questionnaires were collected from passengers who used the self-check-in kiosks in Kuala Lumpur International Airport (KLIA and KLIA2). The collected data were analysed using the structural equation modelling (SEM) technique.

    Findings: Different factors determine passengers' willingness and adoption of SSTs. Perceived ease of use and perceived usefulness significantly affect passenger adoption and behaviour of SSTs in airports. However, the passenger was much comfortable with the SST as the moderating effect of need for human interaction shows a negative result.

    Practical implications: The findings contribute to an understanding of how and why passengers use SSTs, which is critical from a customer relationship management (CRM) perspective. Better strategies can be developed to manage and coordinate SSTs delivery in the airport by understanding the passengers' experience from the self-check-in kiosks.

    Originality/value: This paper goes beyond the basic SSTs usage and intentions study by highlighting the nonimportance of human interaction in SSTs usage specifically by airport passengers.

    MeSH terms: Humans; Surveys and Questionnaires; Technology; Disease Management; Intention; Airports
  3. Wang AY, Akizawa T, Bavanandan S, Hamano T, Liew A, Lu KC, et al.
    Kidney Int Rep, 2019 Nov;4(11):1523-1537.
    PMID: 31890994 DOI: 10.1016/j.ekir.2019.09.007
    Improving Global Outcomes (KDIGO) Clinical Practice Guideline on Chronic Kidney Disease-Mineral and Bone Disorder (CKD-MBD) 2009 provided recommendations on the detection, evaluation, and treatment of CKD-MBD in patients CKD who are and are not undergoing dialysis. Because of the accumulation of evidence since this initial publication, the CKD-MBD Guideline underwent a selective update in 2017. In April 2018, KDIGO convened a CKD-MBD Guideline Implementation Summit in Japan with the key objective to discuss various barriers to the uptake and implementation of the CKD-MBD Guideline in 8 Asian countries/regions. These countries/regions were comparable according to their high-to-middle economic ranking assigned by the World Bank. The discussion took into account the availability of CKD-MBD medication therapies and government health policies that may influence reimbursement and practice patterns in the region. Most importantly, Summit participants developed a framework of multifaceted strategies aimed at overcoming barriers to guideline implementation. The Summit attendees suggested a shared decision-making approach between clinicians and patients in CKD-MBD management, as well as individualized care based on the treatment risk-benefit ratio. The Summit participants also discussed how KDIGO, as a guideline development organization, may work in partnership with local and national nephrology societies to provide education and facilitate implementation of the guideline by clinicians. The conclusions drawn from this Summit in Asia may serve as an important guide for other regions to follow.
  4. Lasekan O, Teoh LS
    BMC Chem, 2019 Dec;13(1):133.
    PMID: 31891159 DOI: 10.1186/s13065-019-0650-3
    Background: The aroma chemistry and the contribution of the aroma compounds to the anti-oxidative properties of roasted yam have yet to be characterized. The growing popularity of roasted yam in regions where they are being consumed calls for a concerted effort to elucidate their aroma chemistry as well as their anti-oxidative properties.

    Results: The aroma compounds in roasted white yam (Dioscorea rotundata) were isolated and identified using static headspace-gas chromatography-mass spectrometry (SH-GC-MS) and gas chromatography-olfactometry (GC-O). In addition, the anti-oxidative activities of the most abundant volatile heterocyclic compounds (2 pyrroles, 4 furans and 3 pyrazines) were evaluated on their inhibitory effect towards the oxidation of hexanal for a period of 30 days. Twenty-nine aroma-active compounds with a flavour dilution (FD) factor range of 2-256 and an array of odour notes were obtained. Among them, the highest odour activities (FD ≥ 128) factors were determined for 2-acetyl furan and 2-acetylpyrrole. Other compounds with significant FD factors ≥ 32 were; 2-methylpyrazine, ethyl furfural, and 5-hydroxy methyl furfural.

    Conclusion: Results of the anti-oxidative activity showed that the pyrroles exhibited the greatest antioxidant activity among all the tested heterocyclic compounds. This was followed by the furans and the pyrazines which had the least antioxidant activity.

    MeSH terms: Aldehydes; Antioxidants; Flavoring Agents; Furaldehyde; Furans; Gas Chromatography-Mass Spectrometry; Pyrazines; Pyrroles; Dioscorea; Olfactometry
  5. Mohd Jusoh NH, Subki A, Yeap SK, Yap KC, Jaganath IB
    BMC Chem, 2019 Dec;13(1):134.
    PMID: 31891160 DOI: 10.1186/s13065-019-0653-0
    Background: Safety, environmental and economic setbacks are driving industries to find greener approaches to extract bioactive compounds from natural resources. Pressurized hot water extraction (PHWE) is among the solvent free and efficient methods for extracting bioactive compounds.

    Experimental: In this study, the suitability of PHWE for extracting bioactive compounds such as phenolics, hydrolysable tannins and flavonoids from Phyllanthus tenellus was investigated by UPLC-qTOF-MS.

    Results: Solvent properties of water are significantly increased through imposing temperature at 121 °C and pressure at 15 p.s.i. Pressurized hot water extraction obtained 991-folds higher hydrolysable tannins than methanol extraction.

    Conclusion: The extraction yields of hydrolysable tannins with PHWE was almost double of absolute methanol extraction.

    MeSH terms: Natural Resources; Methanol; Flavonoids; Phenols; Plant Extracts; Solvents; Temperature; Water; Phyllanthus; Hydrolyzable Tannins
  6. Mohamed Sohaimi N, Bejo MH, Omar AR, Ideris A, Mat Isa N
    PLoS One, 2019;14(12):e0225863.
    PMID: 31891571 DOI: 10.1371/journal.pone.0225863
    Fowl adenovirus (FAdV) is the causative agent of inclusion body hepatitis (IBH) in chickens with significant economic losses due to high mortality and poor production. It was objectives of the study to attenuate and determine the molecular characteristic of FAdV isolate (UPM1137) of Malaysia passages in primary chicken embryo liver (CEL) cells. The cytopathic effect (CPE) was recorded and the present of the virus was detected by polymerase chain reaction (PCR). Nucleotide and amino acid changes were determined and a phylogenetic tree was constructed. The pathogenicity and immunogenicity of the virus at passage 35 (CEL35) with virus titre of 106.7TCID50/mL was determined in day old specific pathogen free (SPF) chicks via oral or subcutaneous route of inoculation. The study demonstrated that the FAdV isolate was successfully propagated and attenuated in CEL cells up to 35th consecutive passages (CEL35) with delayed of CPE formation within 48 to 72 post inoculation (pi) from CEL20 onwards. The virus caused typical CPE with basophilic intranuclear inclusion bodies, refractile and clumping of cells. The virus is belong to serotype 8b with substitution of amino acid at position 44, 133 and 185 in L1 loop of hexon gene and in knob of fiber gene at position 348 and 360 at CEL35. It is non-pathogenic, but immunogenic in SPF chickens. It was concluded that the FAdV isolate was successfully attenuated in CEL cells with molecular changes in major capsid proteins which affect its infectivity in cell culture and SPF chickens.
    MeSH terms: Aviadenovirus; Amino Acids; Animals; Chick Embryo; Chickens; Hepatitis A; Malaysia; Nucleotides; Phylogeny; Specific Pathogen-Free Organisms; Virulence; Polymerase Chain Reaction; Viral Load; Capsid Proteins; Intranuclear Inclusion Bodies; Serogroup
  7. Haricharan PB, Barad N, Patil CR, Voruganti S, Mudrakola DP, Turagam N
    Eur J Dent, 2019 Oct;13(4):503-509.
    PMID: 31891967 DOI: 10.1055/s-0039-1696894
    OBJECTIVES:  The main purpose of this article is to evaluate the clinical performance of atraumatic restorative treatment (ART) sealant versus Embrace WetBond sealant in terms of retention and fissure caries prevention among a section of school children in the southern Indian state of Karnataka (ClinicalTrials.gov NCT02716558).

    MATERIALS AND METHODS:  After obtaining consent from the parents and screening the children against the inclusion cum exclusion criteria, 90 school children whose mandibular first molars were caries free and with a well-defined pit and fissure system were recruited for this spilt mouth trial. The respective molars were allocated either to the ART sealant or the embrace group after the randomization process. The sealants were applied according to the manufacturer's instructions and followed up at the end of 3rd, 6th, and 12th month, respectively. The outcome measures assessed were the retention and the caries preventive effects of the materials.

    RESULTS:  At the end of the 6th month, about one-fourth of the sealants in either group remained totally intact, without evidence of caries. No significant differences were seen between the sealants either in terms of retention or caries preventive benefits at the end of 12 months.

    STATISTICAL ANALYSIS:  The chi-squared test was used to check differences in proportions. The significance value was set at <0.05. Kappa test was performed to assess the intraexaminer reproducibility with respect to retention and caries status.

    CONCLUSION:  The moisture-tolerant resin sealant could not replicate the physical properties usually associated with conventional resin sealants. The usage of ART sealants was deemed to be less cumbersome in an outreach setting as observed in this trial.

    MeSH terms: Child; Humans; India; Informed Consent; Molar; Mouth; Parents; Pit and Fissure Sealants; Random Allocation; Reproducibility of Results; Dental Atraumatic Restorative Treatment
  8. Omar F, Ab-Ghani Z, Rahman NA, Halim MS
    Eur J Dent, 2019 Oct;13(4):589-598.
    PMID: 31891975 DOI: 10.1055/s-0039-1700659
    OBJECTIVES:  This study evaluates the efficacy and safety of the professionally prescribed and nonprescription over-the-counter (OTC) bleaching agents.

    MATERIALS AND: METHODS:  Extracted human upper central incisors were prepared and stained with red wine for 14 days before being subjected to four different bleaching agents: professionally prescribed opalescence PF 15%, VOCO Perfect Bleach 10%, nonprescription OTC Crest 3D Whitestrips, and Whitelight Teeth Whitening System. Colorimetric measurement was performed with Vita Easyshade Handheld Spectrophotometer, enamel surface microhardness measured using Vickers Hardness machine, and surface roughness was evaluated with profilometer, before and after bleaching. Scanning electron microscope (SEM) evaluation and atomic force microscopy were conducted postbleaching.

    STATISTICAL ANALYSIS:  The data were analyzed with t-test, two-way ANOVA, one-way ANOVA, and Turkey's test at a significance level of 5%.

    RESULTS:  All bleaching products have the same efficacy to whiten stained enamel. Opalescence PF 15% showed significant increase in the microhardness (92.69 ± 68.316). All groups demonstrated significant increase in surface roughness (p < 0.05). SEM evaluation showed that Opalescence PF 15% resulted in same microscopic appearance as unbleached enamel, while VOCO Perfect Bleach 10%, Whitelight Teeth Whitening System and Crest 3D Whitestrips demonstrated mild to moderate irregularities and accentuated irregularities, respectively.

    CONCLUSION:  Professionally prescribed bleaching agent of Opalescence PF 15% is effective tin whitening the teeth, while the other bleaching products may be effective but also have deleterious effects on the enamel.

    MeSH terms: Iridescence; Analysis of Variance; Colorimetry; Dental Enamel; Hardness; Humans; Hydrogen Peroxide; Incisor; Microscopy, Electron, Scanning; Tin; Tooth Bleaching; Turkey; Wine; Microscopy, Atomic Force; Bleaching Agents
  9. Faezah Hassan, Siew, Mooi Ching, Poh, Cheng Ling, NurHidayah Jaffar, Kai, Wei Lee
    MyJurnal
    Introduction: Proper gestational diabetes mellitus (GDM) care is essential for optimal control and thus prevents adverse perinatal outcomes. This audit aimed to determine the quality of GDM care provided by a public polyclinic.
    Methods: The audit was performed on the clinic-based medical record of GDM patients who had undergone at least
    three prenatal follow-ups and one postnatal follow-up between January and November 2012. Patients with pre-existing diabetes mellitus were excluded. Results: A total of 74 medical records were audited and it showed that 94.6%
    of patients were of age 25 and above, 91.9% were Malays, 71.6% were multigravida and 98.6% had risk factors of
    GDM. 54.1% of diagnoses were made during the second trimester, while 25.7% and 20.2% of cases were detected
    during the first and third trimester respectively. All of the GDM patients had their weight and blood pressure monitored, 85.1% had their fundal height checked as scheduled and 85.1% were referred for diet counselling. 81.9% and
    100% of GDM patients who were not on treatment and on treatment correspondingly had their blood sugar profile
    monitoring done as recommended. Only 13.5% of GDM patients were given prenatal family planning counselling
    and 35.1% were given a six weeks’ postnatal oral glucose tolerance test appointment. Monthly ultrasound screening
    and HbA1c monitoring was done in merely 51.4% and 58.1% of the GDM patients respectively. Conclusion: The
    GDM care process in this public polyclinic could be improved further to achieve the standard recommendations.
  10. Thambiah, Subashini, Nor Shaibah Ramley, Nur Syazwani Ghazali, Ng, Ooi Chuan, Intan Nureslyna Samsudin, Siti Yazmin Zahari Sham
    MyJurnal
    Introduction: The prevalence of diabetes mellitus (DM) in Malaysia is drastically increasing. Subjects with DM are
    more likely to have deranged liver function tests (LFT). This study aimed to determine the prevalence of abnormal liver enzymes [(alanine aminotransferase (ALT) and alkaline phosphatase (ALP)] and its associated factors among type
    2 DM (T2DM) subjects visiting a referral diabetic clinic in a tertiary government hospital. Methods: This retrospective, cross-sectional study included electronic data of 300 T2DM subjects ≥18 years old in the outpatient specialist
    clinic from January 2011 to December 2014. Statistical analysis was performed using SPSS version 22. Results: The
    study population at large included Malays, of age >60 years with comparable gender percentage. Most subjects had
    long-standing DM, poor glycaemic control and were on treatment. The prevalence of abnormal ALT and ALP was
    27.3% and 13%; with 90.2% and 97.4% having mild ALT and ALP elevations, respectively. Significant associations
    noted for age, body mass index (BMI) and duration of T2DM for ALT whereas for ALP, anti-diabetic medication was
    significant between groups of normal and abnormal levels. Deranged liver enzymes were associated significantly
    with dyslipidaemia. Conclusion: Our study on the crude prevalence of raised liver enzymes may help identify T2DM
    patients at increased risk of non-alcoholic fatty liver disease (NAFLD). Modification of metabolic risk factors, such
    as weight loss, control of dyslipidaemia rather than just tighter glycaemic control should be emphasised to reduce
    morbidity and mortality. Liver enzymes remain a simple and non- invasive marker of liver pathology in daily medical
    practice.
  11. Kang, Nien How, Norashikin Shamsudin
    MyJurnal
    Introduction: Acne vulgaris is not only associated with physical morbidity, but also associated with significant psychosocial disturbances, such as depression and suicide. The objective of this study was to examine the functional
    disability and psychological impact of acne vulgaris. Methods: A cross-sectional study of 55 acne patients, aged
    more than 12, was conducted in the dermatology clinic of Serdang Hospital from January to March 2017. Data on
    demographics, clinical features, current treatment modalities were collected. Acne severity was graded using Comprehensive Acne Severity Scale (CASS), Cardiff Acne Disability Index (CADI) was used to assess functional disability,
    while DASS-21 was used to assess three related states of depression, anxiety and stress. Statistical analysis was done
    using SPSS software. A p value ≤ 0.05 considered significant. Results: The mean age was 23.2 (14-46), majority were
    females (39, 70.9%), Malays (44, 80%) and students (34, 61.8%). 53 (96.4%) subjects had almost clear (CASS = 1) to
    moderate (CASS = 3) acne severity. Majority had facial acne with or without truncal involvement, 53 (96.3%). The
    average age of disease onset was 17.8 (9-45). 45 (81.8%) patients found their acne mild to moderately disabling.
    69.1%, 45.8% and 41.8% reported feeling anxious, depressed and stressed out. CADI was found to be significantly
    related with depression (p=0.012), anxiety (p= 0.015) and stress (p=0.001). Relationship between CASS with CADI
    (p=0.07), stress (p=0.09), anxiety (p=0.13) and depression (p=0.12) were insignificant. Conclusion: Although acne
    vulgaris is not hazardous, it is associated with psychosocial disturbances. Early and effective treatments are necessary, especially when dealing with facial lesions.
  12. Leong, Whye Sook, Nur Izzah Aini Sablihan, Suriani Ismail, Navin Kumar Devaraj, Ching, Siew Mooi
    MyJurnal
    Introduction: Many Malaysians does not engage in regular physical activity. The objective of this study was to determine the factors associated with the level of physical activity among non-academics staff in the Faculty of Medicine
    and Health Sciences (FMHS), Universiti Putra Malaysia (UPM). Methods: This was a cross-sectional study using a
    self-administered questionnaire consisting socio-demographic, International Physical Activity Questionnaires (short
    form) and Depression, Anxiety and Stress Scale (DASS) scales to measure the level of physical activity and association
    with psychosocial and environmental factors. Ethical approval was obtained from the Ethics Committee for Research
    Involving Human Subjects (JKEUPM). Data was analysed using the SPSS 20.0. A p value of ≤ 0.05 was considered as
    statistically significant. Results: The response rate was 84% or 120 respondents. 68 (56.7%) respondents was found
    to have a good level of physical activity. Majority of them were women (70 [58.3%]), aged of < 33 years old (72
    [60.0 %]), Malays (113 [94.2%]), and had tertiary education (81[67.5%]). There are significant associations between
    socio-demographic factors [gender (p=0.013), age (p=0.025) and level of education (p=0.020)] and environmental
    factor (performing physical activity at gymnasium (p=0.021)) with the level of physical activity on univariate analysis.
    On multivariate logistic regression, lower educational level (OR 2.96, p=0.016) and performing physical activity at
    gymnasium/sport centres (OR 4.66, p= 0.026) were associated with engaging with physical activity. Conclusion: Majority of the respondents have a good level of physical activity. Interventions should be planned for staffs otherwise
    identified as not having a good level of physical activity.
  13. Siti Munirah Abdul Basir, Radiah Abdul Ghani, Muhammad Ibrahim, Muhammad Muzaffar Ali Khan Khattak, Muhammad Nor Omar, Nor Azwani Mohd Shukri
    MyJurnal
    Introduction: Nutritional requirements increase during lactation. However, maternal dietary intakes of Malaysian
    mothers are subjected to restrictions commonly included in traditional postpartum practices. This study aimed to
    assess the maternal dietary intake status during the recommended six month exclusive breastfeeding (part of which
    included the traditional confinement) period. Methods: Thirty-two Malay mothers aged 18-35 years, who had delivered full-term (at ≥37 weeks) singleton babies and were exclusively breastfeeding, were included in the study.
    Maternal dietary intake was assessed using multiple-pass diet recall on Days 10, 30, 60, 90, 120, 150, and 180,
    postpartum. The average total energy and macronutrient intakes were compared against recommended values. Results: The findings of this study demonstrated that maternal intakes of total energy ranged from 1,500-2,000kcal/day,
    carbohydrate 189-272g/day, protein 58-72g/day, and total fat 32-70g/day. Total calorie intake was the lowest during
    confinement period (Days 10 and 30) compared to the rest of the exclusive breastfeeding period. This is similar with
    total fat consumption. On the other hand, protein intake was the highest during confinement period whereas carbohydrate intake was consistent throughout the six-month period. Despite the increased requirements, intakes of total
    calories, protein, total fat, dietary fibre, and water, did not meet the recommended values throughout the exclusive
    breastfeeding period. Conclusion: Mothers’ inability to fulfil their nutritional requirements during exclusive breastfeeding period may be associated with traditional postpartum dietary practices. Dietary advice with consideration
    for cultural food taboos practiced by local mothers during confinement may help to improve maternal nutritional
    intakes during this crucial time.
  14. Lim, Hui Li, Ling, Miaw Yn, Teh, Chien Huey, Heng, Pei Pei, Kee, Chee Cheong, Sumarni Mohd Ghazali, et al.
    MyJurnal
    Introduction: Self-esteem is important for the development of adolescent’s psychological well-being. The Rosenberg
    Self-Esteem Scale (RSES) is the most commonly used instrument for measuring self-esteem. However, the Malay-language version of the RSES (RSES-M) has not been validated among upper secondary school students in Malaysia.
    Methods: We administered the RSES-M to 3349 students who participated in the Malaysia Adolescent Health Risk
    Behavior (MyAHRB) study. The construct validity of the RSES-M was assessed using exploratory factor analysis while
    internal consistency was evaluated by Cronbach alpha. Results: The study identified two factors in the RSES-M.
    The variance for the first and second factor was 30.32% and 19.91%, respectively, Item 7 (I wish I could have
    more respect to myself) which showed a positive correlation with the positive wording contrasted with the original
    RSES. Such contrary may be due to social and cultural background difference. Conclusion: The translated version
    of RSES-M can be considered as a valid tool to measure self-esteem in upper school going adolescents in Malaysia.
    However, future studies to determine the psychometric properties of item 7 in the Malaysian setting are strongly
    recommended to enhance the validity of RSES-M.
  15. Mamta Vesudave Remesh Kumar, Sherina Mohd Sidik, Rampal, Lekhraj, Siti Irma Fadhilah Ismail, Periasamy, Ummavathy
    MyJurnal
    Introduction: Cancer-patients undergoing chemotherapy experience a high level of depression. The objective of this
    study was to determine the prevalence and predictors of depression in cancer-patients receiving chemotherapy in
    government hospitals in Peninsular Malaysia. Methods: This research was started with a cross-sectional study among
    1356 patients undergoing chemotherapy in 10 government state hospitals in the Peninsular Malaysia. The data were
    collected using self-administered questionnaires including socio-demographic characteristics, severity of cancer,
    depression through Patient Health Questionnaire (PHQ-9), social support using the Multidimensional Scale of Perceived Social Support (MSPSS) and hopelessness using the Beck Hopelessness Scale (BHS).The research also conducted the descriptive statistics to obtain variable percentages and frequencies. Inferential analysis was also conducted by using chi-square or Fisher’s exact test in determining the relations among variables at the level of significance
    where p
  16. Rohayu Hami, Mohd Hashim Mohd Hassan, Azidah Abdul Kadir, Hasanah Che Ismail, Norsa’adah Bachok
    MyJurnal
    Introduction: Geriatric Giants are the impairment that appear in older people. It comprises of impaired cognition,
    urinary incontinence, instability, fall and immobility. The aim of this study is to determine the prevalence of Geriatric Giants among older people living in three different living environment; own home, pondok and old folks home.
    Methods: This comparative study involved 471 respondents from Kelantan. There were 157 participants each from
    own home, pondok and old folks home. The prevalence of Geriatric Giants were determine by 10 minutes comprehensive screening which was developed to diagnose and manage the Geriatric Giants (WHO, 2007) and prevalence
    were compared using chi-square tests or Fisher exact test. Results: Prevalence of dementia among respondents of old
    folks home (11.5%), pondok (6.4%) and own home (0.6%). Prevalence of urinary incontinence among respondents
    of own home (22.3%), old folks home (16.6%) and pondok (14.0%). Prevalence of instability among respondents of
    old folk home (31.2%), pondok (27.4%) and own home (14.0%). Prevalence of fall among respondents of pondok
    (33.1%), old folks home (24.25) and own home (19.7%). Old folk home respondents had the highest prevalence of
    moderate and severe immobility There were significant difference in prevalence of dementia ((Fisher exact p value
  17. Zaidi NE, Shazali NAH, Chor ALT, Osman MA, Ibrahim K, Jaoi-Edward M, et al.
    J Vis Exp, 2019 12 14.
    PMID: 31885381 DOI: 10.3791/60281
    Tumor-associated macrophages (TAMs) have been identified as an important component for tumor growth, invasion, metastasis, and resistance to cancer therapies. However, tumor-associated macrophages can be harmful to the tumor depending on the tumor microenvironment and can reversibly alter their phenotypic characteristics by either antagonizing the cytotoxic activity of immune cells or enhancing anti-tumor response. The molecular actions of macrophages and their interactions with tumor cells (e.g., phagocytosis) have not been extensively studied. Therefore, the interaction between immune cells (M1/M2-subtype TAM) and cancer cells in the tumor microenvironment is now a focus of cancer immunotherapy research. In the present study, a live cell coculture model of induced M1 macrophages and mouse mammary 4T1 carcinoma cells was developed to assess the phagocytic activity of macrophages using a time-lapse video feature using phase-contrast, fluorescent, and differential interference contrast (DIC) microscopy. The present method can observe and document multipoint live-cell imaging of phagocytosis. Phagocytosis of 4T1 cells by M1 macrophages can be observed using fluorescent microscopy before staining 4T1 cells with carboxyfluorescein succinimidyl ester (CFSE). The current publication describes how to coculture macrophages and tumor cells in a single imaging dish, polarize M1 macrophages, and record multipoint events of macrophages engulfing 4T1 cells during 13 h of coculture.
    MeSH terms: Animals; Breast Neoplasms/immunology*; Breast Neoplasms/pathology; Female; Humans; Macrophages/immunology*; Phagocytosis/physiology*; Coculture Techniques/methods*; Cell Line, Tumor; Mice; Tumor Microenvironment
  18. Nur Zati Iwani AK, Jalaludin MY, Wan Mohd Zin RM, Fuziah MZ, Hong JYH, Abqariyah Y, et al.
    Int J Endocrinol, 2019;2019:8586167.
    PMID: 31885562 DOI: 10.1155/2019/8586167
    Metabolic syndrome (MetS) is an important predictor of cardiovascular diseases in adulthood. This study aims to examine the clinical utility of triglyceride to high-density lipoprotein ratio (TG : HDL-C) in identifying cardiometabolic risk and insulin resistance (IR) among children with obesity, in comparison with MetS as defined by the International Diabetes Federation (IDF). Data of 232 children with obesity aged 10-16 years were obtained from our study, MyBFF@school study, conducted between January and December 2014. Children were divided into tertiles of TG : HDL-C ratio. The minimum value of the highest tertile was 1.11. Thus, elevated TG : HDL-C ratio was defined as TG : HDL-C ≥1.11. Children with MetS were categorized based on the definition established by the IDF. Out of 232 children, 23 (9.9%) had MetS, out of which 5.6% were boys. Almost twofold of boys and girls had elevated TG : HDL-C ratio compared to MetS: 13.8% vs. 5.6% and 13.8% vs. 4.3%, respectively. Children with elevated TG : HDL-C ratio had lower fasting glucose compared to children with MetS (boys = 5.15 ± 0.4 vs. 6.34 ± 2.85 mmol/l, p=0.02; girls = 5.17 ± 0.28 vs. 6.8 ± 4.3 mmol/l, p=0.03). Additionally, boys with elevated TG : HDL-C ratio had a higher HDL-C level compared to those with MetS (1.08 ± 0.18 vs. 0.96 ± 0.1 mmol/l, p=0.03). There was no significant difference across other MetS-associated risk factors. Overall, TG : HDL-C ratio demonstrated higher sensitivity (42.7% vs. 12.9%) but lower specificity (74.8% vs. 93.2%) than MetS in identifying IR, either in HOMA-IR ≥2.6 for prepubertal children or HOMA-IR ≥4 for pubertal children. TG : HDL-C ratio in children with obesity is thus as useful as the diagnosis of MetS. It should be considered an additional component to MetS, especially as a surrogate marker for IR.
  19. Kamsani NE, Zakaria ZA, Md Nasir NL, Mohtarrudin N, Mohamad Alitheen NB
    PMID: 31885651 DOI: 10.1155/2019/5207958
    Methanol extract of Melastoma malabathricum (MEMM) has been traditionally used by the Malay to treat various ailments. In an attempt to develop the plant as an herbal product, MEMM was subjected to the subacute and subchronic toxicity and cytotoxicity studies. On the one hand, the subacute study was performed on three groups of male and three groups of female rats (n = 6), which were orally administered with 8% Tween 80 (vehicle control group) or MEMM (500 and 1000 mg/kg) daily for 28 days, respectively. On the other hand, the subchronic study was performed on four groups of rats (n = 6), which were orally administered with 8% Tween 80 (vehicle control group) or MEMM (50, 250, and 500 mg/kg) daily for 90 days, respectively. In the in vitro study, the cytotoxic effect of MEMM against the HT29 colon cancer cell line was assessed using the MTT assay. MEMM was also subjected to the UHPLC-ESI-HRMS analysis. The results demonstrated that MEMM administration did not cause any mortality, irregularity of behaviour, modification in body weight, as well as food and water intake following the subacute and subchronic oral treatment. There were no significant differences observed in haematological parameters between treatment and control groups in both studies, respectively. The in vitro study demonstrated that MEMM exerts a cytotoxic effect against the HT29 colon cancer cell line when observed under the inverted and phase-contrast microscope and confirmed by the acridine orange/propidium iodide (AOPI) staining. The UHPLC-ESI-HRMS analysis of MEMM demonstrated the occurrence of several compounds including quercetin, p-coumaric acid, procyanidin A, and epigallocatechin. In conclusion, M. malabathricum leaves are safe for oral consumption either at the subacute or subchronic levels and possess cytotoxic action against the HT29 colon cancer cells possibly due to the synergistic action of several flavonoid-based compounds.
    MeSH terms: Acridine Orange; Methanol; Animals; Catechin; Chromatography, High Pressure Liquid; Drinking; Female; Malaysia; Male; Polysorbates; Propidium; Quercetin; Control Groups; Proanthocyanidins; Biflavonoids; Rats
  20. Mustafa MZ, Zulkifli FN, Fernandez I, Mariatulqabtiah AR, Sangu M, Nor Azfa J, et al.
    PMID: 31885664 DOI: 10.1155/2019/8258307
    This study was conducted to evaluate the effects of stingless bee honey (SBH) supplementation on memory and learning in mice. Despite many studies that show the benefits of honey on memory, reports on the nootropic effects of SBH are still lacking, and their underlying mechanism is still unclear. SBH is a honey produced by the bees in the tribe of Meliponini that exist in tropical countries. It features unique storage of honey collected in cerumen pots made of propolis. This SBH may offer a better prospect for therapeutic performance as the previous report identifies the presence of antioxidants that were greater than other honey produced by Apis sp. In this study, SBH was tested on Swiss albino mice following acute (7 days) and semichronic (35 days) supplementation. Experiments were then conducted using Morris water maze (MWM) behaviour analysis, RT-PCR for gene expression of mice striatum, and NMR for metabolomics analysis of the honey. Results indicate spatial working memory and spatial reference memory of mice were significantly improved in the honey-treated group compared with the control group. Improved memory consolidations were also observed in prolonged supplementation. Gene expression analyses of acutely treated mice demonstrated significant upregulation of BDNF and Itpr1 genes that involve in synaptic function. NMR analysis also identified phenylalanine, an essential precursor for tyrosine that plays a role at the BDNF receptor. In conclusion, SBH supplementation for seven days at 2000 mg/kg, which is equivalent to a human dose of 162 mg/kg, showed strong capabilities to improve spatial working memory. And prolonged intake up to 35 days increased spatial reference memory in the mice model. The phenylalanine in SBH may have triggered the upregulation of BDNF genes in honey-treated mice and improved their spatial memory performance.
    MeSH terms: Memory Consolidation; Animals; Antioxidants; Bees; Cerumen; Honey; Humans; Memory, Short-Term; Phenylalanine; Propolis; Tyrosine; Water; Up-Regulation; Nootropic Agents; Brain-Derived Neurotrophic Factor; Receptor, trkB; Control Groups; Mice; Spatial Memory
External Links