Browse publications by year: 2020

  1. Phei-Lian, Wang, Edmund Sim, Ui Hang
    MyJurnal
    Increasing evidence of the association between ribosomal protein (RP) genes with nasopharyngeal carcinoma (NPC) have been derived from findings of their differential expression patterns in NPC cell lines. Nevertheless, expression data from a comprehensive list of RP gene family members is still lacking. This paper reports the assessment of two RP genes, eL13 and eL14, with regards to their expression patterns in several NPC cell lines (TW04, TW01, HK1, HONE1 and SUNE-1) relative to a non-malignant control (NP69). A conventional Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) assay was employed. Analysis of eL13 has never been explored before this, whereas investigation of eL14 represents an extended study. We found a general over-expression trend of eL14 in 40% (2 of 5; TW01 and HONE-1) of the NPC cell lines studied, with higher upregulated level in only one (TW01) of them. However, this pattern of expression level is not statistically significant. Expression of eL13 was not detected in any of the cell lines used. The inconsistency of these expression patterns demonstrates an elusive nature of RP activities in the malignancy of the nasopharynx.
    MeSH terms: Cell Line; Nasopharyngeal Neoplasms; Nasopharynx; RNA-Directed DNA Polymerase; Ribosomal Proteins; Transcriptional Activation; Up-Regulation; Reverse Transcriptase Polymerase Chain Reaction
  2. Jesmie, Babai, Rosdi, Kira, Fazia, Mohd Sinang, Lesley Maurice, Bilung
    MyJurnal
    Vibrio parahaemolyticus is a causative agent of foodborne outbreaks associated with the consumption of raw or under-cooked seafood. This study aimed to quantify and detect the occurrence of V. parahaemolyticus in freshwater fish by performing Most Probable Number (MPN) method in combination with Polymerase Chain Reaction (PCR). In this study, a total of 20 red tilapia (Oreochromis sp.) were collected from nearby local wet markets. Polymerase Chain Reaction (PCR) assay targeting the toxR gene in V. parahaemolyticus was performed, with the expected DNA amplification size of 368 bp. MPN analysis showed that the estimated microbial load of V. parahaemolyticus were more than 1100 MPN/g. The result of the PCR assay confirmed the presence of V. parahaemolyticus in 90% of the isolates. This positive detection elucidated the presence of food-borne bacteria in freshwater fish from local wet-market which may affect not only the health of fish stocks but also raise public health concerns.
    MeSH terms: Animals; Cooking; Disease Outbreaks; DNA; Fresh Water; Public Health; Vibrio parahaemolyticus; Polymerase Chain Reaction; Tilapia
  3. Chowdhury MEH, Khandakar A, Ahmed S, Al-Khuzaei F, Hamdalla J, Haque F, et al.
    Sensors (Basel), 2020 Oct 02;20(19).
    PMID: 33023097 DOI: 10.3390/s20195637
    Growing plants in the gulf region can be challenging as it is mostly desert, and the climate is dry. A few species of plants have the capability to grow in such a climate. However, those plants are not suitable as a food source. The aim of this work is to design and construct an indoor automatic vertical hydroponic system that does not depend on the outside climate. The designed system is capable to grow common type of crops that can be used as a food source inside homes without the need of large space. The design of the system was made after studying different types of vertical hydroponic systems in terms of price, power consumption and suitability to be built as an indoor automated system. A microcontroller was working as a brain of the system, which communicates with different types of sensors to control all the system parameters and to minimize the human intervention. An open internet of things (IoT) platform was used to store and display the system parameters and graphical interface for remote access. The designed system is capable of maintaining healthy growing parameters for the plants with minimal input from the user. The functionality of the overall system was confirmed by evaluating the response from individual system components and monitoring them in the IoT platform. The system was consuming 120.59 and 230.59 kWh respectively without and with air conditioning control during peak summer, which is equivalent to the system running cost of 13.26 and 25.36 Qatari Riyal (QAR) respectively. This system was circulating around 104 k gallons of nutrient solution monthly however, only 8-10 L water was consumed by the system. This system offers real-time notifications to alert the hydroponic system user when the conditions are not favorable. So, the user can monitor several parameters without using laboratory instruments, which will allow to control the entire system remotely. Moreover, the system also provides a wide range of information, which could be essential for plant researchers and provides a greater understanding of how the key parameters of hydroponic system correlate with plant growth. The proposed platform can be used both for quantitatively optimizing the setup of the indoor farming and for automating some of the most labor-intensive maintenance activities. Moreover, such a monitoring system can also potentially be used for high-level decision making, once enough data will be collected. This work presents significant opportunities for the people who live in the gulf region to produce food as per their requirements.
    MeSH terms: Agriculture; Air Conditioning; Brain; Climate; Decision Making; Humans; Running; Water; Hydroponics; Crops, Agricultural; Plant Development
  4. Kong SY, Wong LS, Paul SC, Miah MJ
    Polymers (Basel), 2020 Oct 02;12(10).
    PMID: 33023168 DOI: 10.3390/polym12102270
    This paper investigated the static behaviour of glass fibre reinforced polymer (GFRP) built-up hollow and concrete filled built-up beams tested under four-point bending with a span-to-depth ratio of 1.67, therefore focusing their shear performance. Two parameters considered for hollow sections were longitudinal web stiffener and strengthening at the web-flange junction. The experimental results indicated that the GFRP hollow beams failed by web crushing at supports; therefore, the longitudinal web stiffener has an insignificant effect on improving the maximum load. Strengthening web-flange junctions using rectangular hollow sections increased the maximum load by 47%. Concrete infill could effectively prevent the web crushing, and it demonstrated the highest load increment of 162%. The concrete filled GFRP composite beam failed by diagonal tension in the lightweight concrete core. The finite element models adopting Hashin damage criteria yielded are in good agreement with the experimental results in terms of maximum load and failure mode. Based on the numerical study, the longitudinal web stiffener could prevent the web buckling of the slender GFRP beam and improved the maximum load by 136%. The maximum load may be further improved by increasing the thickness of the GFRP section and the size of rectangular hollow sections used for strengthening. It was found that the bond-slip at the concrete-GFRP interface affected the shear resistance of concrete-GFRP composite beam.
    MeSH terms: Carmustine; Etoposide; Glass; Polymers; Finite Element Analysis; Physical Phenomena
  5. Hooper C, Debnath PP, Biswas S, van Aerle R, Bateman KS, Basak SK, et al.
    Viruses, 2020 10 02;12(10).
    PMID: 33023199 DOI: 10.3390/v12101120
    Mass mortalities of the larval stage of the giant freshwater prawn, Macrobrachium rosenbergii, have been occurring in Bangladesh since 2011. Mortalities can reach 100% and have resulted in an 80% decline in the number of hatcheries actively producing M. rosenbergii. To investigate a causative agent for the mortalities, a disease challenge was carried out using infected material from a hatchery experiencing mortalities. Moribund larvae from the challenge were prepared for metatranscriptomic sequencing. De novo virus assembly revealed a 29 kb single‑stranded positive-sense RNA virus with similarities in key protein motif sequences to yellow head virus (YHV), an RNA virus that causes mass mortalities in marine shrimp aquaculture, and other viruses in the Nidovirales order. Primers were designed against the novel virus and used to screen cDNA from larvae sampled from hatcheries in the South of Bangladesh from two consecutive years. Larvae from all hatcheries screened from both years were positive by PCR for the novel virus, including larvae from a hatchery that at the point of sampling appeared healthy, but later experienced mortalities. These screens suggest that the virus is widespread in M. rosenbergii hatchery culture in southern Bangladesh, and that early detection of the virus can be achieved by PCR. The hypothesised protein motifs of Macrobrachium rosenbergii golda virus (MrGV) suggest that it is likely to be a new species within the Nidovirales order. Biosecurity measures should be taken in order to mitigate global spread through the movement of post-larvae within and between countries, which has previously been linked to other virus outbreaks in crustacean aquaculture.
    MeSH terms: Animals; Bangladesh/epidemiology; Fresh Water/virology*; Larva/virology*; Phylogeny; RNA Virus Infections/mortality*; RNA Virus Infections/veterinary*; RNA Viruses/classification*; RNA Viruses/genetics; RNA Viruses/isolation & purification*; Open Reading Frames; Sequence Alignment; Aquaculture; Nodaviridae/genetics; Nodaviridae/isolation & purification; Palaemonidae/virology*
  6. Khezri R, Hosseini S, Lahiri A, Motlagh SR, Nguyen MT, Yonezawa T, et al.
    Int J Mol Sci, 2020 Oct 02;21(19).
    PMID: 33023274 DOI: 10.3390/ijms21197303
    Zinc-air batteries (ZABs) offer high specific energy and low-cost production. However, rechargeable ZABs suffer from a limited cycle life. This paper reports that potassium persulfate (KPS) additive in an alkaline electrolyte can effectively enhance the performance and electrochemical characteristics of rechargeable zinc-air flow batteries (ZAFBs). Introducing redox additives into electrolytes is an effective approach to promote battery performance. With the addition of 450 ppm KPS, remarkable improvement in anodic currents corresponding to zinc (Zn) dissolution and limited passivation of the Zn surface is observed, thus indicating its strong effect on the redox reaction of Zn. Besides, the addition of 450 ppm KPS reduces the corrosion rate of Zn, enhances surface reactions and decreases the solution resistance. However, excess KPS (900 and 1350 ppm) has a negative effect on rechargeable ZAFBs, which leads to a shorter cycle life and poor cyclability. The rechargeable ZAFB, using 450 ppm KPS, exhibits a highly stable charge/discharge voltage for 800 cycles. Overall, KPS demonstrates great promise for the enhancement of the charge/discharge performance of rechargeable ZABs.
    MeSH terms: Air; Electrolytes/pharmacology; Electrolytes/chemistry*; Electric Power Supplies*; Sulfates/pharmacology; Sulfates/chemistry*; Zinc/chemistry*; Potassium Compounds/pharmacology; Potassium Compounds/chemistry*
  7. Kazeminia M, Abdi A, Shohaimi S, Jalali R, Vaisi-Raygani A, Salari N, et al.
    Head Face Med, 2020 Oct 06;16(1):22.
    PMID: 33023617 DOI: 10.1186/s13005-020-00237-z
    BACKGROUND: Early childhood caries (ECC) is a type of dental caries in the teeth of infants and children that is represented as one of the most prevalent dental problems in this period. Various studies have reported different types of prevalence of dental caries in primary and permanent teeth in children worldwide. However, there has been no comprehensive study to summarize the results of these studies in general, so this study aimed to determine the prevalence of dental caries in primary and permanent teeth in children in different continents of the world during a systematic review and meta-analysis.

    METHODS: In this review study, articles were extracted by searching in the national and international databases of SID, MagIran, IranMedex, IranDoc, Cochrane, Embase, ScienceDirect, Scopus, PubMed, and Web of Science (ISI) between 1995 and December 2019. Random effects model was used for analysis and heterogeneity of studies was evaluated by using the I2 index. Data were analyzed by using the Comprehensive Meta-Analysis (Version 2) software.

    FINDINGS: In this study, a total of 164 articles (81 articles on the prevalence of dental caries in primary teeth and 83 articles on the prevalence of dental caries in permanent teeth) were entered the meta-analysis. The prevalence of dental caries in primary teeth in children in the world with a sample size of 80,405 was 46.2% (95% CI: 41.6-50.8%), and the prevalence of dental caries in permanent teeth in children in the world with a sample size of 1,454,871 was 53.8% (95% CI: 50-57.5%). Regarding the heterogeneity on the basis of meta-regression analysis, there was a significant difference in the prevalence of dental caries in primary and permanent teeth in children in different continents of the world. With increasing the sample size and the year of study, dental caries in primary teeth increased and in permanent teeth decreased.

    CONCLUSION: The results of this study showed that the prevalence of primary and permanent dental caries in children in the world was found to be high. Therefore, appropriate strategies should be implemented to improve the aforementioned situation and to troubleshoot and monitor at all levels by providing feedback to hospitals.

    MeSH terms: Child; Child, Preschool; Dental Caries; Humans; Infant; Regression Analysis; Software; Sudden Infant Death; Tooth, Deciduous; Bibliometrics; Prevalence; Sample Size; Dentition, Permanent; PubMed
  8. Chu YM, Ali R, Asjad MI, Ahmadian A, Senu N
    Sci Rep, 2020 Oct 06;10(1):16643.
    PMID: 33024149 DOI: 10.1038/s41598-020-73174-1
    In this work, influence of hybrid nanofluids (Cu and [Formula: see text]) on MHD Maxwell fluid due to pressure gradient are discussed. By introducing dimensionless variables the governing equations with all levied initial and boundary conditions are converted into dimensionless form. Fractional model for Maxwell fluid is established by Caputo time fractional differential operator. The dimensionless expression for concentration, temperature and velocity are found using Laplace transform. As a result, it is found that fluid properties show dual behavior for small and large time and by increasing volumetric fraction temperature increases and velocity decreases respectively. Further, we compared the Maxwell, Casson and Newtonian fluids and found that Newtonian fluid has greater velocity due to less viscosity. Draw the graphs of temperature and velocity by Mathcad software and discuss the behavior of flow parameters and the effect of fractional parameters.
  9. Jamaludin S, Azmir NA, Mohamad Ayob AF, Zainal N
    Ann Med Surg (Lond), 2020 Nov;59:165-170.
    PMID: 33024558 DOI: 10.1016/j.amsu.2020.09.046
    The COVID-19 outbreak from the SARS-CoV-2 virus has shocking us with its fast transmission and deadly complication. Due to that, the movement restriction has been enforced to contain this pandemic. Recently, there is an increasing pressure to restart and resurrect social and economic sectors, and to allow people to get back to work. This must be well planned before the movement restriction is lifted. Because of that, this paper aims to review and make recommendations on the new normal for our daily activities and works. Firstly, the social and economic sectors must be opening in phases by emphasizing safety and health than an economic recovery. In the meantime, the WHO recommendations on social distancing and personal hygiene must be adapted and become a new normal. Because of that, the government and local authorities should develop a soft landing approach based on the WHO recommendations. Next, the community must be engaged and empowered to do their parts in preventing the spread of COVID-19. From the new normal recommendations, the people can continue their daily routines, and at the same time can reduce COVID-19 transmission. However, medical possibilities are not considered while compiling these new normals and procedures. The population must adapt and embrace the new normal to control, reduce and prevent the spreading of COVID-19, as it could be with us for a long time.
    MeSH terms: Disease Outbreaks; Government; Group Processes; Humans; Hygiene; Social Distance; Pandemics; Return to Work
  10. Shivanna MM, Ganesh S, Khanagar SB, Naik S, Divakar DD, Al-Kheraif AA, et al.
    World J Clin Cases, 2020 Sep 26;8(18):3999-4009.
    PMID: 33024756 DOI: 10.12998/wjcc.v8.i18.3999
    BACKGROUND: Atraumatic restorative treatment (ART) may be appropriate for populations without accessibility and affordability. More data are required regarding the success rate of ART in anterior teeth.

    AIM: To evaluate the clinical performance of restoring class III cavities in anterior teeth of permanent dentition using the ART approach.

    METHODS: A longitudinal interventional field study was carried out at two rural primary health centers, Tumkur district, India. A total of 54 teeth in 39 patients were evaluated for the survival rate of class III restorations in permanent anterior teeth using the ART approach in children and adult populations. Evaluation of ART restoration was carried out using Frencken J criteria, the mean procedure time, patient acceptance and reported pain severity during the ART approach were evaluated using a visual analog scale. Calculation of the cost of ART was also performed.

    RESULTS: The mean time taken to perform the ART procedure was 14.79 ± 5.8 min with the majority of patients reporting only mild pain. At 6 mo follow-up, 72.2% remained in a good state, but this reduced to 27% at 12 mo. The cumulative survival rate of the restorations was 94.4% at 6 mo and 80.9% at 12 mo follow-up. Estimation of the direct cost for a single class III restoration was 186.50 INR (2.64 USD).

    CONCLUSION: ART may be a good comprehensive option for basic oral health care for underserved or inaccessible populations, and preventive care for patients.

    MeSH terms: Adult; Behavior Therapy; Child; Counseling; Follow-Up Studies; Humans; India; Longitudinal Studies; Oral Health; Pain; Research; Survival Rate; Dentition, Permanent; International Normalized Ratio; Dental Atraumatic Restorative Treatment; Visual Analog Scale
  11. Ab Razak S, Mad Radzuan S, Mohamed N, Nor Azman NHE, Abd Majid AM, Ismail SN, et al.
    Heliyon, 2020 Sep;6(9):e05077.
    PMID: 33024864 DOI: 10.1016/j.heliyon.2020.e05077
    The trend of microsatellite marker discovery and development revolved as a result of the advancement of next generation sequencing (NGS) technology as it has developed numerous microsatellites within a short period of time at a low cost. This study generated microsatellite markers using RAD sequencing technologies for the understudied Nephelium lappaceum. A total of 1403 microsatellite markers were successfully designed, which consisted of 853 di-, 525 tri-, 17 tetra-, 5 penta-, and 3 hexanucleotide microsatellite markers. Subsequently, selection of 39 microsatellites was made for the evaluation of genetic diversity of the selected 22 rambutan varieties. Twelve microsatellites, which exhibited high call rates across the samples, were used to assess the diversity of the aforementioned rambutan varieties. The analysis of 12 microsatellites revealed the presence of 72 alleles and six alleles per locus in average. Furthermore, the polymorphic information content (PIC) value ranged from 0.326 (NlaSSR20) to 0.832 (NlaSSR32), which included an average of 0.629 per locus, while the generated Neighbour Joining dendrogram showed two major clusters. The pairwise genetic distance of shared alleles exhibited a range of values from 0.046 (R134↔R170) to 0.818 (R5↔R170), which suggested highest dissimilarity detected between R5 and R170. Notably, these research findings would useful for varietal identification, proper management and conservation of the genetic resources, and exploitation and utilization in future breeding programs.
    MeSH terms: Alleles; Breeding; Oligosaccharides; Genetic Variation; Microsatellite Repeats; Rubiaceae; Sapindaceae; High-Throughput Nucleotide Sequencing
  12. Low CC, Ong LY, Koo VC, Leow MC
    Heliyon, 2020 Sep;6(9):e05107.
    PMID: 33024875 DOI: 10.1016/j.heliyon.2020.e05107
    Digital signage is widely utilized in digital-out-of-home (DOOH) advertising for marketing and business. Recently, the combination of the digital camera and digital signage enables the advertiser to gather the audience demographic for audience measurement. Audience measurement is useful for the advertiser to understand the audience's behavior and improve their business strategies. When an audience is facing the digital display, the vision-based DOOH system will process the audience's face and broadcast a personalized advertisement. Most of the digital signage is available in an uncontrolled environment of public areas. Thus, it poses two main challenges for the vision-based DOOH system to track the audience's movement, which are multiple adjacent faces and occlusion by passer-by. In this paper, a new framework is proposed to combine the digital signage with a depth camera for tracking multi-face in the three-dimensional (3D) environment. The proposed framework extracts the audience's face centroid position (x, y) and depth information (z) and plots into the aerial map to simulate the audience's movement that is corresponding to the real-world environment. The advertiser can further measure the advertising effectiveness through the audience's behavior.
    MeSH terms: Commerce; Demography; Movement; Plant Extracts; Marketing; Optical Devices
  13. Masduki F, Y JM, Min CC, Karim M
    Curr Microbiol, 2020 Dec;77(12):3962-3968.
    PMID: 33025182 DOI: 10.1007/s00284-020-02228-4
    In this study, we aimed to isolate, identify and characterize lactic acid bacteria (LAB) from the intestine of juvenile seabass (Lates calcarifer) as a new potential probiotic. Four strains of LABs were isolated from the intestines of ten healthy seabass juveniles. In the in vitro screening process using spot lawn assay, one isolate labeled as LAB3 showed inhibitory activity against Vibrio harveyi (ATCC 35,084). Strain LAB3 was determined to belong to the gram positive bacteria group with cocci shape and was identified as Enterococcus hirae using 16S rDNA analysis. This bacterium was able to grow at pH ranging from pH 2 to 10 with the best growth at pH 7. This strain was also able to grow at 0-4% NaCl after 24 h incubation and grew best at 1.5% NaCl. Enterococcus hirae strain LAB3 of the present study is worthy to be further characterized as a potential probiotic for use in seabass culture.
    MeSH terms: Animals; Bass*; Intestines; Vibrio; Probiotics*; Enterococcus hirae
  14. Mousa MA
    J Contemp Dent Pract, 2020 Jun 01;21(6):678-682.
    PMID: 33025938
    AIMS: The purpose of this study was to assess the influence of hot and dry weather on the hardness and surface roughness of four different maxillofacial silicone elastomeric materials (MFSEM) including two room-temperature vulcanized (RTV) and two high-temperature vulcanized (HTV) materials.

    MATERIALS AND METHODS: Eighty test specimens were fabricated according to the manufacturer's instructions into rectangular test specimens. The hardness and surface roughness were tested, after 6 months of exposure to natural hot and dry weather. The hardness was measured through the International Rubber Hardness Degree (IRHD) scale using an automated hardness tester. The surface roughness was measured using a novel 3D optical noncontact technique using a combination of a light sectioning microscope and a computer vision system. Statistical Package for Social Sciences software SPSS/version 24 was used for analysis and a comparison between two independent variables was done using an independent t test, while more than two variables were analyzed, F test (ANOVA) to be used followed by a post hoc test to determine the level of significance between every two groups.

    RESULTS: The hot and dry weather statistically influenced the hardness and surface roughness of MFSEM. Cosmesil M-511 showed the least hardness in test groups while A-2000 showed the hardest material (p < 0.05). A-2000 showed significant changes from rough in case of nonweathered to become smoother in weather followed by A-2186 (p < 0.05). Cosmesil M-511 showed the roughest material.

    CONCLUSION: Cosmesil M-511 showed the least hard MFSEM after outdoor weathering while A-2000, the highest and least material showed hardness and surface roughness, respectively.

    CLINICAL IMPLICATION: A-2000 had a high IRHD scale hardness. This makes this material more suitable for the replacement of ear and nose defects. Cosmesil M-511 is soft and easily adaptable material that makes the material more appropriate for the replacement of small facial defect with undercut area to be easily inserted and removed. Whilst A-2000 is smoother and finer in test specimens after weathering, Cosmesil M-511 became rougher after weathering.

    MeSH terms: Hardness; Materials Testing; Maxillofacial Prosthesis*; Surface Properties; Weather
  15. Ogliari G, Lunt E, Ong T, Marshall L, Sahota O
    Arch Osteoporos, 2020 10 07;15(1):156.
    PMID: 33026586 DOI: 10.1007/s11657-020-00825-1
    We investigated whether osteoporotic fractures declined during lockdown, among adults aged 50 years and older. We showed that fewer outpatients attended the Fracture Clinic, for non-hip fractures, during lockdown; in contrast, no change in admissions for hip fractures was observed. This could be due to fewer outdoors falls, during lockdown.

    PURPOSE: Many countries implemented a lockdown to control the spread of the COVID-19 pandemic. We explored whether outpatient attendances to the Fracture Clinic for non-hip fragility fracture and inpatient admissions for hip fracture declined during lockdown, among adults aged 50 years and older, in a large secondary care hospital.

    METHODS: In our observational study, we analysed the records of 6681 outpatients attending the Fracture Clinic, for non-hip fragility fractures, and those of 1752 inpatients, admitted for hip fracture, during the time frames of interest. These were weeks 1st to 12th in 2020 ("prior to lockdown"), weeks 13th to 19th in 2020 ("lockdown") and corresponding periods over 2015 to 2019. We tested for differences in mean numbers (standard deviation (SD)) of outpatients and inpatients, respectively, per week, during the time frames of interest, across the years.

    RESULTS: Prior to lockdown, in 2020, 63.1 (SD 12.6) outpatients per week attended the Fracture Clinic, similar to previous years (p value 0.338). During lockdown, 26.0 (SD 7.3) outpatients per week attended the Fracture Clinic, fewer than previous years (p value < 0.001); similar findings were observed in both sexes and age groups (all p values < 0.001). During lockdown, 16.1 (SD 5.6) inpatients per week were admitted for hip fracture, similar to previous years (p value 0.776).

    CONCLUSION: During lockdown, fewer outpatients attended the Fracture Clinic, for non-hip fragility fractures, while no change in inpatient admissions for hip fracture was observed. This could reflect fewer non-hip fractures and may inform allocation of resources during pandemic.

    MeSH terms: Aged; Aged, 80 and over; Female; Hip Fractures/epidemiology*; Hospitalization/statistics & numerical data; Humans; Male; Middle Aged; Pneumonia, Viral/epidemiology*; Coronavirus Infections/epidemiology*; Osteoporotic Fractures/epidemiology*; Pandemics
  16. Joginder Singh S, Gan B, Chu SY
    J Dev Behav Pediatr, 2020 10 8;42(2):146-151.
    PMID: 33027107 DOI: 10.1097/DBP.0000000000000873
    OBJECTIVE: Language and symbolic play require the same underlying symbolic ability and develop parallel to one another. The aim of this study was to determine the association between symbolic play and vocabulary in typically developing children and children with Down syndrome in Malaysia.

    METHODS: Twenty-five typically developing children and 25 children with Down syndrome aged between 12 and 36 months were involved in this study. They were recruited from an early intervention center and various kindergartens from the West Coast of Peninsular Malaysia. Their play skills were assessed using the Symbolic Play Test Second Edition, and information about their vocabulary was obtained through the MacArthur Bates Communicative Development Inventories that was filled out by their parents.

    RESULTS: There was a significant difference in the vocabulary and symbolic play scores of children with Down syndrome compared with typically developing children. There was also a positive correlation between symbolic play scores and receptive and expressive vocabulary scores for both groups of children.

    CONCLUSION: When providing intervention, speech-language pathologists need to promote the development of symbolic play in addition to language, given the association between the two. They should also look into introducing an augmentative and alternative communication system to the children who demonstrate age-appropriate symbolic play skills but have trouble with symbolic language production.

    MeSH terms: Child; Child, Preschool; Communication Disorders*; Down Syndrome*; Humans; Infant; Language; Language Development; Language Tests; Vocabulary
  17. Mbous YP, Hayyan M, Wong WF, Hayyan A, Looi CY, Hashim MA
    J Phys Chem B, 2020 10 15;124(41):9086-9094.
    PMID: 32930594 DOI: 10.1021/acs.jpcb.0c04801
    Deep eutectic solvent (DES) affinities with cellular membranes structures dictate the degree of cytotoxicity that results from these interactions. The physicochemical properties of choline chloride (ChCl)-DESs suggest non-negligible cytotoxicities that were attested by published researches. In this study, the profiles of novel N,N-diethylammonium chloride (DAC)-based-deep eutectic solvents (DESs) prepared with various hydrogen bond donors (urea, glycerol, ethylene glycol, malonic acid, and zinc chloride) were compared to those of ChCl-DESs by using HelaS3, AGS, MCF-7, and WRL-68 cancer cell lines. The molecular interactions between salts and cellular membranes were investigated to explain the observed cytotoxicity. The results show that ChCl-based DESs (279 ≤ IC50 ≥ 1260 mM) were less toxic than DAC-based DESs (37 ≤ IC50 ≥ 109 mM). COSMO-RS analysis emphasized the importance of salt hydrophobicity with regards to DESs cytotoxicity. Malonic acid increased hydrophobicity and cytotoxicity in general, thus highlighting the potential of ammonium salt-based DESs as anticancer agents.
    MeSH terms: Choline*; Glycerol; Hydrogen Bonding; Neoplasms*; Solvents; Ethylene Glycol
  18. Aziz SB, Brza MA, Hamsan EMADMH, Hadi JM, Kadir MFZ, Abdulwahid RT
    Molecules, 2020 Oct 01;25(19).
    PMID: 33019618 DOI: 10.3390/molecules25194503
    Plasticized magnesium ion conducting polymer blend electrolytes based on chitosan (CS): polyvinyl alcohol (PVA) was synthesized with a casting technique. The source of ions is magnesium triflate Mg(CF3SO3)2, and glycerol was used as a plasticizer. The electrical and electrochemical characteristics were examined. The outcome from X-ray diffraction (XRD) examination illustrates that the electrolyte with highest conductivity exhibits the minimum degree of crystallinity. The study of the dielectric relaxation has shown that the peak appearance obeys the non-Debye type of relaxation process. An enhancement in conductivity of ions of the electrolyte system was achieved by insertion of glycerol. The total conductivity is essentially ascribed to ions instead of electrons. The maximum DC ionic conductivity was measured to be 1.016 × 10-5 S cm-1 when 42 wt.% of plasticizer was added. Potential stability of the highest conducting electrolyte was found to be 2.4 V. The cyclic voltammetry (CV) response shows the behavior of the capacitor is non-Faradaic where no redox peaks appear. The shape of the CV response and EDLC specific capacitance are influenced by the scan rate. The specific capacitance values were 7.41 F/g and 32.69 F/g at 100 mV/s and 10 mV/s, respectively. Finally, the electrolyte with maximum conductivity value is obtained and used as electrodes separator in the electrochemical double-layer capacitor (EDLC) applications. The role of lattice energy of magnesium salts in energy storage performance is discussed in detail.
    MeSH terms: Crystallization; Electrolytes/chemistry*; Glycerol/chemistry; Ions; Magnesium/chemistry*; Polyvinyl Alcohol/chemistry*; Salts/chemistry*; X-Ray Diffraction; Electric Impedance; Electric Capacitance*; Chitosan/chemistry*; Electrochemical Techniques*
  19. Mori H, Schol J, Geeraerts A, Huang IH, Jandee S, Gonlachanvit S, et al.
    J Clin Med, 2020 Oct 01;9(10).
    PMID: 33019626 DOI: 10.3390/jcm9103189
    BACKGROUND: The new coronavirus disease (COVID-19) has high infection and mortality rates, and has become a pandemic. The infection and mortality rates are lower in Asian countries than in European countries. This study aimed to conduct a survey on the effects of COVID-19 on the capacity to perform gastrointestinal motility tests in Asian countries compared with European countries.

    METHODS: We used the questionnaire previously established by our team for researchers in European countries. The correlation between the decreased rate of gastrointestinal motility and function tests, and the infection/mortality rates of COVID-19 and stringency of a government's interventions in each country was analysed and protective measures were assessed.

    RESULTS: In total, 58 gastroenterologists/motility experts in Asian countries responded to this survey. The infection/mortality rates of COVID-19 and Stringency Index had a significant impact on the testing capacity of oesophageal manometry and catheter-based pH monitoring. In European countries, most facilities used filtering facepiece 2/3 (FFP2/3) masks during oesophageal motility studies. Meanwhile, in Asian countries, most facilities used surgical masks.

    CONCLUSION: The total infection and mortality rates of COVID-19 can affect the rate of gastrointestinal motility testing and the type of protective equipment that must be used.

    MeSH terms: Asia; Europe; Gastrointestinal Motility; Gastrointestinal Transit; Government; Humans; Hydrogen-Ion Concentration; Manometry; Protective Devices; Surveys and Questionnaires; Coronavirus; Coronavirus Infections; Pandemics; Gastroenterologists
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