Browse publications by year: 2020

  1. Xin KY, Eusufzai SZ, Jamayet NB, Alam MK
    Work, 2020;67(1):165-171.
    PMID: 32955481 DOI: 10.3233/WOR-203262
    BACKGROUND: Accidental occupational injuries increase the risk of communicable diseases like hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) among dental personnel. Such occupational injuries affect healthcare quality by enhancing dissatisfaction and lack of motivation to work among dental personnel.

    OBJECTIVE: The present study aims to assess knowledge and awareness regarding needle stick and sharp injury among dental personnel and compared the knowledge and awareness level about needle stick injuries between dental professionals and dental supporting staff working at the School of Dental Sciences, Universiti Sains Malaysia.

    METHODS: This cross-sectional study was conducted from March 2016 to March 2017. The total sample included 112 registered dental personnel including dental professionals (lecturers, dental officers, and postgraduate students) and dental supportive staff (including staff nurses and dental surgery assistant) who were selected from the School of Dental Sciences, Universiti Sains Malaysia at Kelantan District of Malaysia. Data were collected by a structured questionnaire.

    RESULTS: In our study 60.7% respondents answered correctly for all the statements regarding knowledge and 51.8% of the respondents answered correctly for the statements regarding awareness on needle stick and sharp injury. No significant difference of knowledge level has been observed between the dental professionals and dental supportive staff (p 

    MeSH terms: Cross-Sectional Studies; Dentists*; Humans; Health Knowledge, Attitudes, Practice*; Malaysia
  2. Liew YT, Junior ACG, Bakri A, Narayanan P
    Ci Ji Yi Xue Za Zhi, 2020 04 01;32(3):303-304.
    PMID: 32955511 DOI: 10.4103/tcmj.tcmj_245_19
  3. Mollica MA, Mayer DK, Oeffinger KC, Kim Y, Buckenmaier SS, Sivaram S, et al.
    JCO Glob Oncol, 2020 09;6:1394-1411.
    PMID: 32955943 DOI: 10.1200/GO.20.00180
    PURPOSE: The purpose of this study was to describe follow-up care for breast and colorectal cancer survivors in countries with varying levels of resources and highlight challenges regarding posttreatment survivorship care.

    METHODS: We surveyed one key stakeholder from each of 27 countries with expertise in survivorship care on questions including the components/structure of follow-up care, delivery of treatment summaries and survivorship care plans, and involvement of primary care in survivorship. Descriptive analyses were performed to characterize results across countries and variations between the WHO income categories (low, middle, high). We also performed a qualitative content analysis of narratives related to survivorship care challenges to identify major themes.

    RESULTS: Seven low- or /lower-middle-income countries (LIC/LMIC), seven upper-middle-income countries (UMIC), and 13 high-income countries (HICs) were included in this study. Results indicate that 44.4% of countries with a National Cancer Control Plan currently address survivorship care. Additional findings indicate that HICs use guidelines more often than those in LICs/LMICs and UMICs. There was great variation among countries regardless of income level. Common challenges include issues with workforce, communication and care coordination, distance/transportation issues, psychosocial support, and lack of focus on follow-up care.

    CONCLUSION: This information can guide researchers, providers, and policy makers in efforts to improve the quality of survivorship care on a national and global basis. As the number of cancer survivors increases globally, countries will need to prioritize their long-term needs. Future efforts should focus on efforts to bridge oncology and primary care, building international partnerships, and implementation of guidelines.

    MeSH terms: Administrative Personnel; Aftercare; Breast Neoplasms; Communication; Developing Countries; Humans; Income; Poverty; World Health Organization; Colorectal Neoplasms; Survivors; Developed Countries; Psychosocial Support Systems
  4. Chhablani J, Wong K, Tan GS, Sudhalkar A, Laude A, Cheung CMG, et al.
    Asia Pac J Ophthalmol (Phila), 2020;9(5):426-434.
    PMID: 32956188 DOI: 10.1097/APO.0000000000000312
    PURPOSE: The aim of this consensus article was to provide comprehensive recommendations in the management of diabetic macular edema (DME) by reviewing recent clinical evidence.

    DESIGN: A questionnaire containing 47 questions was developed which encompassed clinical scenarios such as treatment response to anti-vascular endothelial growth factor and steroid, treatment side effects, as well as cost and compliance/reimbursement in the management of DME using a Dephi questionnaire as guide.

    METHODS: An expert panel of 12 retinal specialists from Singapore, Malaysia, Philippines, India and Vietnam responded to this questionnaire on two separate occasions. The first round responses were compiled, analyzed and discussed in a round table discussion where a consensus was sought through voting. Consensus was considered achieved, when 9 of the 12 panellists (75%) agreed on a recommendation.

    RESULTS: The DME patients were initially profiled based on their response to treatment, and the terms target response, adequate response, nonresponse, and inadequate response were defined. The panellists arrived at a consensus on various aspects of DME treatment such as need for classification of patients before treatment, first-line treatment options, appropriate time to switch between treatment modalities, and steroid-related side effects based on which recommendations were derived, and a treatment algorithm was developed.

    CONCLUSIONS: This consensus article provides comprehensive, evidence-based treatment guidelines in the management of DME in Asian population. In addition, it also provides recommendations on other aspects of DME management such as steroid treatment for stable glaucoma patients, management of intraocular pressure rise, and recommendations for cataract development.

    MeSH terms: Diabetic Retinopathy/complications; Diabetic Retinopathy/diagnosis; Diabetic Retinopathy/drug therapy*; Follow-Up Studies; Glucocorticoids/administration & dosage*; Humans; Macular Edema/diagnosis; Macular Edema/drug therapy*; Macular Edema/etiology; Surveys and Questionnaires; Retrospective Studies; Visual Acuity*; Treatment Outcome; Angiogenesis Inhibitors/administration & dosage*; Consensus*; Vascular Endothelial Growth Factor A/antagonists & inhibitors; Intravitreal Injections
  5. Chong ZX, Yeap SK, Ho WY
    Arch Biochem Biophys, 2020 11 30;695:108583.
    PMID: 32956633 DOI: 10.1016/j.abb.2020.108583
    miRNAs are short non-coding RNA molecules that regulate the expression of mRNA post-transcriptionally. MiRNAs that are secreted into the circulation, also termed circulating miRNAs, have been studied extensively for their roles in diagnosis, treatment and prognosis of human breast cancer. Breast cancer is the most prevalent female cancer and is associated with key cancer hallmarks including sustained proliferation, evasion of apoptosis, increased invasion, enhanced metastases, initation of inflammation, induction of angiogenesis, metabolic derangement and immune dysregulation. This review aimed to explore the relationships between circulating miRNAs and different breast cancer hallmarks. Besides, the advantages, challenges and clinical application of using circulating miRNAs in human breast cancer management were also discussed.
    MeSH terms: Breast Neoplasms/blood*; Breast Neoplasms/immunology; Breast Neoplasms/pathology; Breast Neoplasms/therapy; Female; Humans; Inflammation/blood; Inflammation/immunology; Inflammation/pathology; Inflammation/therapy; Neoplasm Invasiveness; Neoplasm Metastasis; Neovascularization, Pathologic/blood*; Neovascularization, Pathologic/immunology; Neovascularization, Pathologic/pathology; Neovascularization, Pathologic/therapy; RNA, Neoplasm/blood*; RNA, Neoplasm/immunology; Apoptosis*; Cell Proliferation*
  6. Ahmad P, Khandaker MU, Muhammad N, Rehman F, Ullah Z, Khan G, et al.
    Appl Radiat Isot, 2020 Dec;166:109404.
    PMID: 32956924 DOI: 10.1016/j.apradiso.2020.109404
    The shortcomings in Boron neutron capture therapy (BNCT) and Hyperthermia for killing the tumor cell desired for the synthesis of a new kind of material suitable to be first used in BNCT and later on enable the conditions for Hyperthermia to destroy the tumor cell. The desire led to the synthesis of large band gap semiconductor nano-size Boron-10 enriched crystals of hexagonal boron nitride (10BNNCs). The contents of 10BNNCs are analyzed with the help of x-ray photoelectron spectroscopy (XPS) and counter checked with Raman and XRD. The 10B-contents in 10BNNCs produce 7Li and 4He nuclei. A Part of the 7Li and 4He particles released in the cell is allowed to kill the tumor (via BNCT) whereas the rest produce electron-hole pairs in the semiconductor layer of 10BNNCs suggested to work in Hyperthermia with an externally applied field.
    MeSH terms: Animals; Boron/therapeutic use; Boron/chemistry; Boron Compounds/chemical synthesis*; Boron Compounds/therapeutic use; Boron Compounds/chemistry; Humans; Hyperthermia, Induced/methods; Isotopes/therapeutic use; Isotopes/chemistry; Neoplasms/radiotherapy; Neoplasms/therapy; Spectrum Analysis, Raman; X-Ray Diffraction; Boron Neutron Capture Therapy/methods*; Nanotechnology; Quantum Dots/therapeutic use; Quantum Dots/ultrastructure; Quantum Dots/chemistry; Microscopy, Electron, Transmission; Nanoparticles/therapeutic use; Nanoparticles/ultrastructure; Nanoparticles/chemistry*; Photoelectron Spectroscopy
  7. Salleh NS, Abdullah KL, Yoong TL, Jayanath S, Husain M
    J Pediatr Nurs, 2020 09 18;55:174-183.
    PMID: 32957021 DOI: 10.1016/j.pedn.2020.09.002
    PROBLEM: Stigma affects not only children diagnosed with autism spectrum disorder (ASD) themselves, but also people connected with them (i.e., parents, siblings). The capacity of parents to provide care is affected by the stigma they perceive. This meta-synthesis encompasses the experiences of affiliate stigma among parents of children with ASD.

    ELIGIBILITY CRITERIA: Articles were limited to the English language, those reported on stigma experienced by parents of children with ASD aged 2-18 years, published between 1940 and 2019.

    SAMPLE: PubMed, CINAHL, PsycINFO, EMBASE, Scopus and The Cochrane Library databases were searched for eligible studies. Titles and abstracts were reviewed, and twelve articles fitted the selection criteria. The texts of the selected research papers were reviewed by two independent reviewers.

    RESULTS: Four common themes across parental experiences included felt stigma, enacted stigma, variations in stigma, and contributors to stigmatizing experiences.

    CONCLUSIONS: Highlighting the differences in parents' views on affiliate stigma is necessary to create awareness about ASD and the stigma linked with this disorder.

    IMPLICATIONS: The findings asserted that healthcare professionals, especially those in pediatric settings, and society need to have a greater awareness of the stigma and challenges that these parents encounter as this has implications on their mental and physical health. This awareness will lead to more compassionate health care delivery which will support them and create a better environment for families and children with ASD.

    MeSH terms: Autism Spectrum Disorder*; Adolescent; Child; Child, Preschool; Humans; Parent-Child Relations; Parents; Qualitative Research; Social Stigma
  8. Aiman Nadia Akmar Rahman, Mohd Nidzam Jawis, Surini Yusoff
    MyJurnal
    Introduction: The Pro12Ala variant of the Peroxisome Proliferator Activated Receptor Gamma (PPARγ) is one of the critical genetic factor predispose to positive energy balance leading to obesity. Objective: This study aimed to determine the association between Pro12Ala variant in the PPARγ gene with body mass index (BMI) status, physical activity and fat intake among Malay children. Methods: A total of 119 participants aged between 9-11 years old from primary schools in Kota Bharu, Kelantan were recruited. Anthropometric measurements were taken and activity counts of the participants were recorded using accelerometer (Actigraph GT3X+). A food diary was distributed to all participants to collect the data of their fat intake. Genotyping was performed using High Resolution Melting (HRM) analysis. Data obtained were analysed using SPSS version 19. Results: There was a significant association between Pro12Ala variant in the PPARγ gene with BMI status. The allelic frequency of wildtype (Ala/ala) and heterozygous (Pro/ala) among overweight group were 0.83 and 0.17 respectively and 0.92 and 0.08 in the normal weight group (p=0.03). There was a significant difference in BMI, waist circumference and hip circumference between heterozygous and wildtype groups. Conclusion: The study found that there was a significant role of Pro12Ala variant in the PPARγ gene in overweight Malay children.
  9. Nurul Syazana Mohamad Shah, Sarina Sulong, Wan Azman Wan Sulaiman, Ahmad Sukari Halim
    MyJurnal
    Introduction: Exome sequencing technology which is part of Next Generation Sequencing (NGS) is known for detection of various disease mutations through commercially available platforms. Less reports in identifying genetic variation in non-syndromic cleft lip with or without cleft palate (NSCL/P) in Malaysia had embarked for discovery of susceptible genes to fill in the gaps with the healthcare delivery for a better treatment and management to the patients and family. Methods: Whole exome sequencing was carried out on two Malay NSCLP patients. Blood samples were withdrawn and intact DNA was extracted, fragmented, purified and hybridized using exome sequencing capture and sequenced with Agilent 2100 Bioanalyzer platform. Bioinformatic analyses were done and reviewed with GenBank and PubMed database. Variants were filtered based upon a high impact variant. Results: We have identified single nucleotide polymorphisms in 2 genes (PDE4DIP and PDE11A) and InDels frameshift mutations in 4 genes (PDE4DIP, LTBP4, MMP12 and MMP28). Our preliminary study presents the successful application of whole exome sequencing to elucidate the genetic basis of NSCLP in Malays. Conclusion: Mutations that have been identified would shed more light on the susceptible genes to non-syndromic clefts and further investigation shall be carried out to confirm.
  10. Nik Norliza Nik Hassan, NurWaliyuddin Hanis Zainal Abidin, Aminatul Alisya Ahmad Saifuddin, Muhd Faiz Azman, Rozita Hassan, Zuliani Mahmood
    MyJurnal
    The rapid progression of molecular-based technology now enables us to analyse huge number of samples. Nonetheless, DNA (Deoxyrebonucleic acid) extraction is always a limiting factor. In this study, we analysed human microbes to compare the performance of DNA extraction methods: simple boiling method and MasterPureTM Complete DNA and RNA Purification Kit. Dental plaque was initially collected from 12 subjects, 6 of which were from individuals with caries active lesions with International Caries Detection and Assessment System (ICDAS) score five and six, and 6 samples from non-caries subjects, were collected in deionised water. The bacterial samples were extracted by the two aforementioned methods and examined using gel electrophoresis, followed by polymerase chain reaction (PCR). Streptococcus mutans was detected using conventional polymerase chain reaction (PCR). Our results demonstrated that boiling method produced higher DNA concentration (100 – 350 ng/µl), however, the commercial kit yield superior in DNA quality with single and specific PCR products. Based on the findings, the commercial kit is the better choice and practicable method for DNA extraction considering the quality of the DNA yield.
  11. Wan Khairunnisa Wan Juhari, Wan Faiziah Wan Abdul Rahman, Andee Dzulkarnaen Zakaria, Khairul Bariah Ahmad Amin Noordin, Bin Alwi Zilfalil
    MyJurnal
    Colorectal cancer (CRC) is one of the most common cancer worldwide with approximately 2 to 5% of all colon cancers are associated with well-defined hereditary factors. Hereditary nonpolyposis colorectal cancer (Hereditary Nonpolyposis Colorectal Cancer), also known as Lynch syndrome (LS), is the most common form of hereditary CRC characterized by an early age of onset and follows the autosomal dominant inheritance pattern. HNPCC is caused by the alteration in four mismatch repair (MMR) genes. Immunohistochemistry (IHC) and microsatellite instability (MSI) testing, followed by conventional Sanger sequencing reliably identify the majority of mutations. However, methods to identify other underlying variants or genomic rearrangements of HNPCC have emerged. In addition to the clinical characterization and evaluation of HNPCC patients, the implementation of screening strategies for both affected and unaffected CRC patients together with the accelerated advancement in molecular testing methods will shed light on a more comprehensive detection of HNPCC. In this review, the approaches for the selection of high-risk HNPCC and molecular testing performed over the past few years are discussed.
  12. Mot Yee Yik, Narazah Mohd Yusoff, Adam Azlan, Yaashini Rajasegaran, Lew Sze Yuen, Ong Si Min, et al.
    MyJurnal
    The human leukaemia develops with abnormal increase of blast cells in the bone marrow. Leukaemia is caused by genetic aberrations which activates proto-oncogenes and inactivates tumor-suppressor genes and eventually leads to leukemogenesis. Myelodysplastic syndrome is a preleukemic state which shares similar symptoms and causative factors as leukaemia. FOXO3 and c-Myc have been increasingly recognized as key regulatory genes involved in the initiation and development of leukaemia and myelodysplastic syndromes. Their roles in these diseases is being investigated and findings thus far has indicated that FOXO3 acts as a tumor suppressor while c-Myc has been identified as a proto-oncogene. Currently published literature indicate that there are limited research on the correlation between FOXO3 and c-Myc especially in leukaemia and myelodysplastic syndrome. This review will focus on the key regulatory roles of FOXO3 and c-Myc in leukaemia and myelodysplastic syndrome.
  13. SharifahNany RahayuKarmilla SyedHassan, Narazah Mohd Yusoff, Bin Alwi Zilfalil
    MyJurnal
    Nill
  14. Ashrafi N, Azmah Hanim MA, Sarraf M, Sulaiman S, Hong TS
    Materials (Basel), 2020 Sep 15;13(18).
    PMID: 32942621 DOI: 10.3390/ma13184090
    Hybrid reinforcement's novel composite (Al-Fe3O4-SiC) via powder metallurgy method was successfully fabricated. In this study, the aim was to define the influence of SiC-Fe3O4 nanoparticles on microstructure, mechanical, tribology, and corrosion properties of the composite. Various researchers confirmed that aluminum matrix composite (AMC) is an excellent multifunctional lightweight material with remarkable properties. However, to improve the wear resistance in high-performance tribological application, hardening and developing corrosion resistance was needed; thus, an optimized hybrid reinforcement of particulates (SiC-Fe3O4) into an aluminum matrix was explored. Based on obtained results, the density and hardness were 2.69 g/cm3, 91 HV for Al-30Fe3O4-20SiC, after the sintering process. Coefficient of friction (COF) was decreased after adding Fe3O4 and SiC hybrid composite in tribology behaviors, and the lowest COF was 0.412 for Al-30Fe3O4-20SiC. The corrosion protection efficiency increased from 88.07%, 90.91%, and 99.83% for Al-30Fe3O4, Al-15Fe3O4-30SiC, and Al-30Fe3O4-20SiC samples, respectively. Hence, the addition of this reinforcement (Al-Fe3O4-SiC) to the composite shows a positive outcome toward corrosion resistance (lower corrosion rate), in order to increase the durability and life span of material during operation. The accomplished results indicated that, by increasing the weight percentage of SiC-Fe3O4, it had improved the mechanical properties, tribology, and corrosion resistance in aluminum matrix. After comparing all samples, we then selected Al-30Fe3O4-20SiC as an optimized composite.
    MeSH terms: Aluminum; Corrosion; Hardness; Longevity; Metallurgy; Powders; Friction; Ferrosoferric Oxide; Nanoparticles
  15. Kaur K, Sulaiman AH, Yoon CK, Hashim AH, Kaur M, Hui KO, et al.
    PMID: 32942770 DOI: 10.3390/ijerph17186730
    Mental health disorders (MHDs) among refugees has been recognized as a major public health issue. However, to date, there is limited evidence on the prevalence of MHDs among Rohingya refugees in Malaysia. This study aimed to examine the prevalence and associated factors of major depressive disorder (MDD), generalized anxiety disorder (GAD), and post-traumatic stress disorder (PTSD) among Rohingya refugees in Malaysia. A total of 220 refugees were randomly selected to participate in this cross-sectional study, conducted from June 2019 to November 2019. Perceived social support, religious orientation, food security, and sociodemographic characteristics were assessed as independent variables. The dependent variables assessed were MDD, GAD, and PTSD. The prevalence of GAD, PTSD, and MDD was reported at 92 (41.8%), 84 (38.2%), and 71 (32.3%). Several factors were significantly associated with MDD following multivariate analysis such as perceived low to moderate social support (AOR = 2.17; 95% CI 1.13, 4.19) and food insecurity (AOR = 2.77; 95% CI 1.19, 6.47). Exposure to violence (AOR = 38.46; 95% CI 16.27, 90.91) and food insecurity (AOR = 3.74; 95% CI 1.41, 9.91) were significantly associated with PTSD. Addressing these risk factors could be key in improving mental health outcomes among this vulnerable population.
    MeSH terms: Cross-Sectional Studies; Female; Humans; Malaysia; Male; Mental Health
  16. Salvamani S, Tan HZ, Thang WJ, Ter HC, Wan MS, Gunasekaran B, et al.
    Br J Biomed Sci, 2020 Oct;77(4):168-184.
    PMID: 32942955 DOI: 10.1080/09674845.2020.1826136
    The COVID-19 disease is caused by the SARS-CoV-2 virus, which is highly infective within the human population. The virus is widely disseminated to almost every continent with over twenty-seven million infections and over ninety-thousand reported deaths attributed to COVID-19 disease. SARS-CoV-2 is a single stranded RNA virus, comprising three main viral proteins; membrane, spike and envelope. The clinical features of COVID-19 disease can be classified according to different degrees of severity, with some patients progressing to acute respiratory distress syndrome, which can be fatal. In addition, many infections are asymptomatic or only cause mild symptoms. As there is no specific treatment for COVID-19 there is considerable endeavour to raise a vaccine against SARS-CoV-2, in addition to engineering neutralizing antibody interventions. In the absence of an effective vaccine, movement controls of varying stringencies have been imposed. Whilst enforced lockdown measures have been effective, they may be less effective against the current strain of SARS-CoV-2, the G614 clade. Conversely, other mutations of the virus, such as the Δ382 variant could reduce the clinical relevance of infection. The front runners in the race to develop an effective vaccine focus on the SARS-Co-V-2 Spike protein. However, vaccines that produce a T-cell response to a wider range of SARS-Co-V-2 viral proteins, may be more effective. Population based studies that determine the level of innate immunity to SARS-CoV-2, from prior exposure to the virus or to other coronaviruses, will have important implications for government imposed movement control and the strategic delivery of vaccination programmes.
    MeSH terms: Humans; Viral Vaccines/therapeutic use; Pandemics*
  17. Krisch S, Browning TJ, Graeve M, Ludwichowski KU, Lodeiro P, Hopwood MJ, et al.
    Sci Rep, 2020 Sep 17;10(1):15230.
    PMID: 32943713 DOI: 10.1038/s41598-020-72100-9
    Climate change has led to a ~ 40% reduction in summer Arctic sea-ice cover extent since the 1970s. Resultant increases in light availability may enhance phytoplankton production. Direct evidence for factors currently constraining summertime phytoplankton growth in the Arctic region is however lacking. GEOTRACES cruise GN05 conducted a Fram Strait transect from Svalbard to the NE Greenland Shelf in summer 2016, sampling for bioessential trace metals (Fe, Co, Zn, Mn) and macronutrients (N, Si, P) at ~ 79°N. Five bioassay experiments were conducted to establish phytoplankton responses to additions of Fe, N, Fe + N and volcanic dust. Ambient nutrient concentrations suggested N and Fe were deficient in surface seawater relative to typical phytoplankton requirements. A west-to-east trend in the relative deficiency of N and Fe was apparent, with N becoming more deficient towards Greenland and Fe more deficient towards Svalbard. This aligned with phytoplankton responses in bioassay experiments, which showed greatest chlorophyll-a increases in + N treatment near Greenland and + N + Fe near Svalbard. Collectively these results suggest primary N limitation of phytoplankton growth throughout the study region, with conditions potentially approaching secondary Fe limitation in the eastern Fram Strait. We suggest that the supply of Atlantic-derived N and Arctic-derived Fe exerts a strong control on summertime nutrient stoichiometry and resultant limitation patterns across the Fram Strait region.
  18. Tan WH, Chai LC, Chin CF
    Physiol Mol Biol Plants, 2020 Sep;26(9):1889-1896.
    PMID: 32943824 DOI: 10.1007/s12298-020-00868-1
    Alpinia belongs to a large genus with many species found in Peninsular Malaysia. Several species of Alpinia exhibit important medicinal potential. However, progressive studies on the genus Alpinia were hampered by difficulties encountered in species identification. With the advancement achieved in genomic technology, more sensitive tools such as DNA barcoding were developed, which can be used for species identification. Internal Transcribe Spacer 2 (ITS2) is a DNA barcode which has proven to be a promising tool for species identification. The criterions of ITS2 efficacy namely universality and efficacy for species identification were tested on Alpinia species collected from Peninsular Malaysia. The results showed that a success rate of 96.97% was achieved using ITS2 for screening 11 species of Alpinia and an outgroup sample (Zingiber specatabile). Combined with 15 additional sequences from the Genbank for five Alpinia species, ITS2 demonstrated high species identification efficacy with 88.2% of species identified using phylogenetic and distance analysis. The analysis was further improved with the use of ITS2 secondary structure. The results of both criterions demonstrated the ability of ITS2 to successfully discriminate Alpinia species, which will help to improve species identification of Alpinia species in Peninsular Malaysia.
  19. Bala AA, Jatau AI, Yunusa I, Mohammed M, Mohammed AH, Isa AM, et al.
    Ther Adv Drug Saf, 2020;11:2042098620935721.
    PMID: 32944213 DOI: 10.1177/2042098620935721
    Introduction: Snakebite envenoming (SBE) is an important occupational and public health hazard especially in sub-Saharan Africa. For optimum management of SBE, adequate knowledge of Snake antivenom (SAV) is very critical among the healthcare practioners in this region. Information related to the knowledge of SAV use in the management of SBE, as well as SAV logistics is scarce among the Health Care Professionals (HCPs) in Nigeria, particularly in the northern region. We therefore aimed to develop, validate and utilize a tool to assess the SAV knowlegde among HCPs in northern Nigeria. We also sought to implement and evaluate an intervention that could improve the SAV knowledge among the HCPs.

    Methods: The proposed study will be conducted in three phases: Phase I will involve the development of the item-pool to be included in the tool, followed by a face, content validity and construct validity. The tool reliability, readability and difficulty index will be determined. Phase II will involve the utilization of the tool to assess baseline SAV knowledge among the HCPs followed by an educational intervention. Multiple Linear Regression analysis will be used to determine the factors associated with SAV knowledge among the HCPs. Lastly, Phase III which will be a repeat of Phase II to assess and evaluate the knowledge after the intervention.

    Discussion: The study design and findings may guide future implementation and streamline the intervention of improving SAV knowledge in HCPs training and practice.

    Lay Summary: Knowledge assessment and educational intervention of snake antivenom among healthcare practitioners in northern Nigeria: a study protocol Snakebite envenoming (SBE) is an important occupational and public health hazard especially in sub-Saharan Africa. For optimum management of SBE, adequate knowledge of snake antivenom (SAV) is very critical among the healthcare practitioners. The baseline knowledge SAV dosage, mode of administration, availability, and logistics is very relevant among healthcare professionals, particularly those that are directly involved in its logistics. It is paramount that SAV is handled and used appropriately. The efforts and advocacy for the availability for more SAV will be in vain if not handled appropriately before they are used. This study protocol aims to develop a tool, to assess SAV knowledge and effects of educational interventions among healthcare professionals (HCPs) in northern Nigeria. This protocol suggests conducting studies in three phases: (a) Development and validation of SAV knowledge assessment tool, (b) Baseline assessment of SAV knowledge assessment tool among HCPs, and (c) Development, implementation and evaluation of an educational intervention to improve SAV knowledge among HCPs in northern Nigeria.

    MeSH terms: Animals; Antivenins; Health Personnel; Humans; Nigeria; Public Health; Regression Analysis; Snake Bites; Snakes; Reproducibility of Results; Multivariate Analysis; Linear Models; Comprehension
  20. Ahmad MS, Suardi N, Shukri A, Nik Ab Razak NNA, Oglat AA, Makhamrah O, et al.
    Eur J Radiol Open, 2020;7:100257.
    PMID: 32944594 DOI: 10.1016/j.ejro.2020.100257
    Introduction: Hepatocellular carcinoma (HCC) is one of the most common cancer in the world, and the effectiveness of its treatment lies in its detection in its early stages. The aim of this study is to mimic HCC dynamically through a liver phantom and apply it in multimodality medical imaging techniques including magnetic resonance imaging (MRI), computed tomography (CT), and ultrasound.

    Methods and materials: The phantom is fabricated with two main parts, liver parenchyma and HCC inserts. The liver parenchyma was fabricated by adding 2.5 wt% of agarose powder combined with 2.6 wt% of wax powder while the basic material for the HCC samples was made from polyurethane solution combined with 5 wt% glycerol. Three HCC samples were inserted into the parenchyma by using three cylinders implanted inside the liver parenchyma. An automatic injector is attached to the input side of the cylinders and a suction device connected to the output side of the cylinders. After the phantom was prepared, the contrast materials were injected into the phantom and imaged using MRI, CT, and ultrasound.

    Results: Both HCC samples and liver parenchyma were clearly distinguished using the three imaging modalities: MRI, CT, and ultrasound. Doppler ultrasound was also applied through the HCC samples and the flow pattern was observed through the samples.

    Conclusion: A multimodal dynamic liver phantom, with HCC tumor models have been fabricated. This phantom helps to improve and develop different methods for detecting HCC in its early stages.

    MeSH terms: Contrast Media; Glycerol; Carcinoma, Hepatocellular; Liver Neoplasms; Magnetic Resonance Imaging; Polyurethanes; Powders; Sepharose; Suction; Tomography, X-Ray Computed; Ultrasonography; Ultrasonography, Doppler; Phantoms, Imaging
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