Browse publications by year: 2020

  1. Aladwani M, Lophatananon A, Robinson F, Rahman A, Ollier W, Kote-Jarai Z, et al.
    PLoS One, 2020;15(9):e0238928.
    PMID: 32941451 DOI: 10.1371/journal.pone.0238928
    INTRODUCTION: Previous evidence has suggested a relationship between male self-reported body size and the risk of developing prostate cancer. In this UK-wide case-control study, we have explored the possible association of prostate cancer risk with male self-reported body size. We also investigated body shape as a surrogate marker for fat deposition around the body. As obesity and excessive adiposity have been linked with increased risk for developing a number of different cancers, further investigation of self-reported body size and shape and their potential relationship with prostate cancer was considered to be appropriate.

    OBJECTIVE: The study objective was to investigate whether underlying associations exist between prostate cancer risk and male self-reported body size and shape.

    METHODS: Data were collected from a large case-control study of men (1928 cases and 2043 controls) using self-administered questionnaires. Data from self-reported pictograms of perceived body size relating to three decades of life (20's, 30's and 40's) were recorded and analysed, including the pattern of change. The associations of self-identified body shape with prostate cancer risk were also explored.

    RESULTS: Self-reported body size for men in their 20's, 30's and 40's did not appear to be associated with prostate cancer risk. More than half of the subjects reported an increase in self-reported body size throughout these three decades of life. Furthermore, no association was observed between self-reported body size changes and prostate cancer risk. Using 'symmetrical' body shape as a reference group, subjects with an 'apple' shape showed a significant 27% reduction in risk (Odds ratio = 0.73, 95% C.I. 0.57-0.92).

    CONCLUSIONS: Change in self-reported body size throughout early to mid-adulthood in males is not a significant risk factor for the development of prostate cancer. Body shape indicative of body fat distribution suggested that an 'apple' body shape was protective and inversely associated with prostate cancer risk when compared with 'symmetrical' shape. Further studies which investigate prostate cancer risk and possible relationships with genetic factors known to influence body shape may shed further light on any underlying associations.

    MeSH terms: Adult; Aged; Aged, 80 and over; Great Britain/epidemiology; Humans; Male; Middle Aged; Prostatic Neoplasms/epidemiology*; Risk Factors; Body Mass Index; Case-Control Studies; Body Size*; Self Report
  2. Alhajj MN, Ariffin Z, Celebić A, Alkheraif AA, Amran AG, Ismail IA
    PLoS One, 2020;15(9):e0239232.
    PMID: 32941532 DOI: 10.1371/journal.pone.0239232
    BACKGROUND: The perception of dentofacial esthetics differs considerably between patients and dentists. Patient's expectations regarding his/her esthetics are important and should be assessed ahead of any planning of esthetic treatment. The aim of this study was to explore the differences in perception of orofacial appearance by laypersons with different social and demographic status.

    MATERIALS AND METHODS: Self-administered questionnaires were distributed to 400 subjects. The questionnaire comprised three parts; the first part addressed the demographic variables. The second part queried about dental prosthesis, orthognathic or plastic surgery, and/or ongoing or previous orthodontic treatment. The third part included the Arabic version of the-8-item Orofacial Esthetic Scale (OES-Ar) whose responses were scored in the 5-point Likert scale. These scores were compared by different grouping factors (age, gender, marital status, and education) using non-parametric Mann-Whitney U and Kruskal Willis tests with 95% confidence interval (α > 0.05).

    RESULTS: A total of 268 questionnaires were eligible for analysis, representing 67% response rate. The satisfaction with facial profile appearance was the highest (4.0±1.1) followed by facial appearance (3.9±1.1), while the color of teeth was the least satisfying item (3.1±1.3). No significant differences were found between age groups for the mean summary score as well as for each item independently. No significant difference was found between both sexes except for the last item "overall impression". Married subjects rated one item (alignment of teeth) better than their counterparts. Positive perception of orofacial appearance increased significantly with the increase of education level, the perception of the oral health status, and the perception of the general health status.

    CONCLUSION: Good oral health and/or high education level are significant determinants of more positive perception of orofacial esthetic appearance. Patients with these characteristics might be more concerned about their orofacial appearance, and this should be taken into consideration before planning any esthetic restorative dental treatment.

    MeSH terms: Adult; Age Factors; Attitude*; Educational Status; Female; Humans; Male; Perception; Surveys and Questionnaires; Sex Factors; Arab World; Dentofacial Deformities/psychology*
  3. Xue Mei L, Mohammadi Nafchi A, Ghasemipour F, Mat Easa A, Jafarzadeh S, Al-Hassan AA
    Int J Biol Macromol, 2020 Dec 01;164:4603-4612.
    PMID: 32941902 DOI: 10.1016/j.ijbiomac.2020.09.082
    The development of intelligent packaging based on natural and biodegradable resources is getting more attention by researchers in recent years. The aim of this study was to develop and characterize a pH-sensitive films based on sago starch and incorporated with anthocyanin from torch ginger. The pH-sensitive films were fabricated by casting method with incorporation of different torch ginger extract (TGE) concentration. The surface morphology, physicochemical, barrier, and mechanical properties as well as the pH-sensitivity of films were investigated. The film with the highest concentration of TGE showed the lowest tensile strength (4.26 N/m2), toughness (2.54 MJ/m3), Young's modulus (73.96 MPa) and water vapour permeability (2.6 × 10-4 g·m/day·kPa·m2). However, its elongation at break (85.14%), moisture content (0.27%) and water solubility (37.92%) were the highest compared to other films. pH sensitivity analysis showed that the films containing TGE extract, changes in colour by changing the pH. The colour of films changed from pink to slightly green as the pH increased from pH 4 to 9. Thus, the developed pH-sensitive film with torch ginger extract has potential as intelligent packaging for detection of food freshness or spoilage to ensure their quality and safe consumption.
    MeSH terms: Anthocyanins/analysis; Anthocyanins/chemistry*; Colorimetry; Hydrogen-Ion Concentration; Manufactured Materials*; Materials Testing; Microscopy, Electron, Scanning; Permeability; Plant Extracts/chemistry*; Solubility; Starch/chemistry*; Tensile Strength; Water; Spectroscopy, Fourier Transform Infrared; Zingiberaceae/chemistry*; Inflorescence/chemistry
  4. Jamal AbuAlRoos N, Azman MN, Baharul Amin NA, Zainon R
    Phys Med, 2020 Oct;78:48-57.
    PMID: 32942196 DOI: 10.1016/j.ejmp.2020.08.017
    PURPOSE: The main objective of this study was to evaluate the efficacy of tungsten carbide as new lead-free radiation shielding material in nuclear medicine by evaluating the attenuation properties.

    MATERIALS AND METHODS: The elemental composition of tungsten carbide was analysed using Field-Emission Scanning Electron Microscopy (FESEM) with energy dispersive X-ray (EDX). The purity of tungsten carbide was 99.9%, APS: 40-50 µm. Three discs of tungsten carbide was fabricated with thickness of 0.1 cm, 0.5 cm and 1.0 cm. Three lead discs with similar thickness were used to compare the attenuation properties with tungsten carbide discs. Energy calibration of gamma spectroscopy was performed by using 123I, 133Ba, 152Eu, and 137Cs. Gamma radiation from these sources were irradiated on both materials at energies ranging from 0.160 MeV to 0.779 MeV. The experimental attenuation coefficients of lead and tungsten carbide were compared with theoretical attenuation coefficients of both materials from NIST database. The half value layer and mean free path of both materials were also evaluated in this study.

    RESULTS: This study found that the peaks obtained from gamma spectroscopy have linear relationship with all energies used in this study. The relative differences between the measured and theoretical mass attenuation coefficients are within 0.19-5.11% for both materials. Tungsten carbide has low half value layer and mean free path compared to lead for all thickness at different energies.

    CONCLUSION: This study shows that tungsten carbide has high potential to replace lead as new lead-free radiation shielding material in nuclear medicine.

    MeSH terms: Gamma Rays; Nuclear Medicine*; Radiation Protection*; Tungsten; X-Rays
  5. Yuan F, Hung RJ, Walsh N, Zhang H, Platz EA, Wheeler W, et al.
    Cancer Res, 2020 Sep 15;80(18):4004-4013.
    PMID: 32641412 DOI: 10.1158/0008-5472.CAN-20-0447
    Registry-based epidemiologic studies suggest associations between chronic inflammatory intestinal diseases and pancreatic ductal adenocarcinoma (PDAC). As genetic susceptibility contributes to a large proportion of chronic inflammatory intestinal diseases, we hypothesize that the genomic regions surrounding established genome-wide associated variants for these chronic inflammatory diseases are associated with PDAC. We examined the association between PDAC and genomic regions (±500 kb) surrounding established common susceptibility variants for ulcerative colitis, Crohn's disease, inflammatory bowel disease, celiac disease, chronic pancreatitis, and primary sclerosing cholangitis. We analyzed summary statistics from genome-wide association studies data for 8,384 cases and 11,955 controls of European descent from two large consortium studies using the summary data-based adaptive rank truncated product method to examine the overall association of combined genomic regions for each inflammatory disease group. Combined genomic susceptibility regions for ulcerative colitis, Crohn disease, inflammatory bowel disease, and chronic pancreatitis were associated with PDAC at P values < 0.05 (0.0040, 0.0057, 0.011, and 3.4 × 10-6, respectively). After excluding the 20 PDAC susceptibility regions (±500 kb) previously identified by GWAS, the genomic regions for ulcerative colitis, Crohn disease, and inflammatory bowel disease remained associated with PDAC (P = 0.0029, 0.0057, and 0.0098, respectively). Genomic regions for celiac disease (P = 0.22) and primary sclerosing cholangitis (P = 0.078) were not associated with PDAC. Our results support the hypothesis that genomic regions surrounding variants associated with inflammatory intestinal diseases, particularly, ulcerative colitis, Crohn disease, inflammatory bowel disease, and chronic pancreatitis are associated with PDAC. SIGNIFICANCE: The joint effects of common variants in genomic regions containing susceptibility loci for inflammatory bowel disease and chronic pancreatitis are associated with PDAC and may provide insights to understanding pancreatic cancer etiology.
    MeSH terms: Celiac Disease/genetics; Chronic Disease; Colitis, Ulcerative/genetics; Crohn Disease/genetics; Humans; Pancreatic Neoplasms/genetics*; Cholangitis, Sclerosing/genetics; Inflammatory Bowel Diseases/genetics*; Case-Control Studies; Genetic Predisposition to Disease*; Carcinoma, Pancreatic Ductal/genetics*; Pancreatitis, Chronic/genetics; Genome-Wide Association Study/statistics & numerical data
  6. Park SE, Paudel P, Wagle A, Seong SH, Kim HR, Fauzi FM, et al.
    J Agric Food Chem, 2020 Sep 30;68(39):10719-10729.
    PMID: 32869630 DOI: 10.1021/acs.jafc.0c04502
    Luteolin, a flavonoid widely distributed in the plant kingdom, contains two benzene rings and hydroxyl groups, and this structural specificity contributes to its diverse biological activities. However, no previous studies have simultaneously investigated the therapeutic potency of luteolin isolated from a plant as an antipsychotic and antidepressant. Here, luteolin exhibited selective inhibition of hMAO-A (IC50 = 8.57 ± 0.47 μM) over hMAO-B (IC50 > 100 μM). In silico proteochemometric modeling predicted promising targets of luteolin, and verification via cell-based G protein-coupled receptor functional assays showed that luteolin is a selective antagonist of the vasopressin receptor V1AR (IC50 = 19.49 ± 6.32 μM) and the dopamine D4 receptor (IC50 = 39.59 ± 1.46 μM). Molecular docking showed the tight binding of luteolin with a low binding score and the high stability of the luteolin-receptor complex, corroborating its functional effect. Thus, hMAO-A, hD4R, and hV1AR are prime targets of luteolin and potential alternatives for the management of neurodegenerative diseases.
    MeSH terms: Antidepressive Agents; Benzene; Flavonoids; Antipsychotic Agents; Receptors, Vasopressin; Neurodegenerative Diseases; Inhibitory Concentration 50; Receptors, G-Protein-Coupled; Luteolin; Receptors, Dopamine D4; Molecular Docking Simulation
  7. Boyle ST, Mittal P, Kaur G, Hoffmann P, Samuel MS, Klingler-Hoffmann M
    J Proteome Res, 2020 10 02;19(10):4093-4103.
    PMID: 32870688 DOI: 10.1021/acs.jproteome.0c00511
    Tumorigenesis involves a complex interplay between genetically modified cancer cells and their adjacent normal tissue, the stroma. We used an established breast cancer mouse model to investigate this inter-relationship. Conditional activation of Rho-associated protein kinase (ROCK) in a model of mammary tumorigenesis enhances tumor growth and progression by educating the stroma and enhancing the production and remodeling of the extracellular matrix. We used peptide matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to quantify the proteomic changes occurring within tumors and their stroma in their regular spatial context. Peptides were ranked according to their ability to discriminate between the two groups, using a receiver operating characteristic tool. Peptides were identified by liquid chromatography tandem mass spectrometry, and protein expression was validated by quantitative immunofluorescence using an independent set of tumor samples. We have identified and validated four key proteins upregulated in ROCK-activated mammary tumors relative to those expressing kinase-dead ROCK, namely, collagen I, α-SMA, Rab14, and tubulin-β4. Rab14 and tubulin-β4 are expressed within tumor cells, whereas collagen I is localized within the stroma. α-SMA is predominantly localized within the stroma but is also expressed at higher levels in the epithelia of ROCK-activated tumors. High expression of COL1A, the gene encoding the pro-α 1 chain of collagen, correlates with cancer progression in two human breast cancer genomic data sets, and high expression of COL1A and ACTA2 (the gene encoding α-SMA) are associated with a low survival probability (COLIA, p = 0.00013; ACTA2, p = 0.0076) in estrogen receptor-negative breast cancer patients. To investigate whether ROCK-activated tumor cells cause stromal cancer-associated fibroblasts (CAFs) to upregulate expression of collagen I and α-SMA, we treated CAFs with medium conditioned by primary mammary tumor cells in which ROCK had been activated. This led to abundant production of both proteins in CAFs, clearly highlighting the inter-relationship between tumor cells and CAFs and identifying CAFs as the potential source of high levels of collagen 1 and α-SMA and associated enhancement of tissue stiffness. Our research emphasizes the capacity of MALDI-MSI to quantitatively assess tumor-stroma inter-relationships and to identify potential prognostic factors for cancer progression in human patients, using sophisticated mouse cancer models.
    MeSH terms: Animals; Breast Neoplasms; Cell Transformation, Neoplastic; Chromatography, Liquid; Extracellular Matrix; Fluorescent Antibody Technique; Humans; Prognosis; ROC Curve; Tubulin; Mammary Neoplasms, Animal; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Collagen Type I; Proteomics; Mice; Tandem Mass Spectrometry; rho-Associated Kinases; Cancer-Associated Fibroblasts
  8. Bhattacharya P, Ellegård R, Khalid M, Svanberg C, Govender M, Keita ÅV, et al.
    Elife, 2020 Sep 02;9.
    PMID: 32876566 DOI: 10.7554/eLife.57869
    HIV transmission via genital and colorectal mucosa are the most common routes of dissemination. Here, we explored the effects of free and complement-opsonized HIV on colorectal tissue. Initially, there was higher antiviral responses in the free HIV compared to complement-opsonized virus. The mucosal transcriptional response at 24 hr revealed the involvement of activated T cells, which was mirrored in cellular responses observed at 96 hr in isolated mucosal T cells. Further, HIV exposure led to skewing of T cell phenotypes predominantly to inflammatory CD4+ T cells, that is Th17 and Th1Th17 subsets. Of note, HIV exposure created an environment that altered the CD8+ T cell phenotype, for example expression of regulatory factors, especially when the virions were opsonized with complement factors. Our findings suggest that HIV-opsonization alters the activation and signaling pathways in the colorectal mucosa, which promotes viral establishment by creating an environment that stimulates mucosal T cell activation and inflammatory Th cells.
    MeSH terms: Adolescent; Adult; Colon/immunology; Colon/virology; Complement System Proteins/immunology; Complement System Proteins/metabolism; Complement System Proteins/chemistry; Complement Activation/immunology*; Female; Humans; Intestinal Mucosa/immunology*; Intestinal Mucosa/virology; Lymphocyte Activation/immunology*; Male; Opsonin Proteins/immunology; Opsonin Proteins/metabolism; Opsonin Proteins/chemistry; T-Lymphocytes/immunology; HIV-1/immunology*; HIV Infections/immunology*; Young Adult
  9. Charles Shapu R, Ismail S, Ahmad N, Lim PY, Abubakar Njodi I
    Foods, 2020 Sep 10;9(9).
    PMID: 32927593 DOI: 10.3390/foods9091265
    Inadequate food security contributes to poor health outcome for all, including adolescent girls. The study aims at determining the level of food security and hygiene among adolescent girls. A school-based cross-sectional study was carried out among adolescent girls aged 10 to 19 years old from six schools in Maiduguri Metropolitan Council, Borno State. The instrument for data collection was a questionnaire using KoBoCollect Toolbox between June and July 2019. Multivariable logistic regression was used to identify the predictors of food security and hygiene among respondents. A majority of the respondents (73.5%) were in a very low food security level. Three factors were significantly associated with food security level, these included schools (p = 0.007), age of mother (p = 0.004), and occupation of mother (p < 0.001). School (p = 0.003), age (father p = 0.017; mother; p = 0.012), hygiene (p = 0.005), and occupation of mother (p = 0.002) were predictors of food security. About (46.6%) of respondents had poor hygiene practice, school (p = 0.016) was significantly associated with hygiene practice. School (p = 0.019; and p = 0.005) and food security (p = 0.009) were predictors of hygiene practice. This study reveals a high prevalence of low food security among adolescent girls.
    MeSH terms: Adolescent; Adult; Child; Cross-Sectional Studies; Fathers; Female; Food Supply; Humans; Hygiene; Male; Mothers; Nigeria; Occupations; Surveys and Questionnaires; Schools; Prevalence; Logistic Models; Young Adult
  10. Mohd Suffian NI, Adznam SN', Abu Saad H, Chan YM, Ibrahim Z, Omar N, et al.
    Nutrients, 2020 Sep 10;12(9).
    PMID: 32927741 DOI: 10.3390/nu12092758
    The ageing process has been associated with various geriatric issues including frailty. Without early prevention, frailty may cause multiple adverse outcomes. However, it potentially may be reversed with appropriate interventions. The aim of the study is to assess the effectiveness of nutritional education and exercise intervention to prevent frailty among the elderly. A 3-month, single-blind, two-armed, cluster randomized controlled trial of the frailty intervention program among Malaysian pre-frail elderly will be conducted. A minimum of total 60 eligible respondents from 8 clusters (flats) of Program Perumahan Rakyat (PPR) flats will be recruited and randomized to the intervention and control arm. The intervention group will receive a nutritional education and a low to moderate multi-component exercise program. To date, this is the first intervention study that specifically targets both the degree of frailty and an improvement in the outcomes of frailty using both nutritional education and exercise interventions among Malaysian pre-frail elderly. If the study is shown to be effective, there are major potential benefits to older population in terms of preventing transition to frailty. The findings from this trial will potentially provide valuable evidence and serve as a model for similar future interventions designed for elderly Malaysians in the community.
    MeSH terms: Aged; Aged, 80 and over; Exercise Therapy/education*; Female; Health Promotion/methods*; Humans; Malaysia; Male; Middle Aged; Quality of Life; Geriatric Assessment; Cluster Analysis; Randomized Controlled Trials as Topic; Single-Blind Method; Frail Elderly*; Nutrition Policy; Independent Living; Healthy Diet*
  11. Han H, Meng B, Chua BL, Ryu HB
    PMID: 32927834 DOI: 10.3390/ijerph17186594
    International volunteer tourism is an emerging and sustainable trend of the global tourism industry. In this study, we attempted to provide a clear comprehension of volunteer tourists' mental health increase and pro-social intention formation. A survey method and quantitative approach were used. Our result from the structural analysis showed that hedonic and utilitarian performances, mental health, and volunteer tourism engagement had significant associations and that these relationships contributed to improving pro-social intention. In addition, results from the metric invariance assessment revealed that the volunteer tourism engagement and pro-social intention relation was under the significant influence of problem awareness and ascribed responsibility. Mental health and engagement acted as significant mediators. The comparative importance of volunteer tourism engagement was uncovered. Overall, our results provided a sufficient understanding of volunteer tourists' pro-social decision-making process and behaviors.
    MeSH terms: Adult; Female; Humans; Male; Mental Health*; Middle Aged; Surveys and Questionnaires; Social Behavior*; Volunteers/psychology*; Intention
  12. Che Othman FE, Yusof N, Yub Harun N, Bilad MR, Jaafar J, Aziz F, et al.
    Polymers (Basel), 2020 Sep 10;12(9).
    PMID: 32927881 DOI: 10.3390/polym12092064
    Various types of activated carbon nanofibers' (ACNFs) composites have been extensively studied and reported recently due to their extraordinary properties and applications. This study reports the fabrication and assessments of ACNFs incorporated with graphene-based materials, known as gACNFs, via simple electrospinning and subsequent physical activation process. TGA analysis proved graphene-derived rice husk ashes (GRHA)/ACNFs possess twice the carbon yield and thermally stable properties compared to other samples. Raman spectra, XRD, and FTIR analyses explained the chemical structures in all resultant gACNFs samples. The SEM and EDX results revealed the average fiber diameters of the gACNFs, ranging from 250 to 400 nm, and the successful incorporation of both GRHA and reduced graphene oxide (rGO) into the ACNFs' structures. The results revealed that ACNFs incorporated with GRHA possesses the highest specific surface area (SSA), of 384 m2/g, with high micropore volume, of 0.1580 cm3/g, which is up to 88% of the total pore volume. The GRHA/ACNF was found to be a better adsorbent for CH4 compared to pristine ACNFs and reduced graphene oxide (rGO/ACNF) as it showed sorption up to 66.40 mmol/g at 25 °C and 12 bar. The sorption capacity of the GRHA/ACNF was impressively higher than earlier reported studies on ACNFs and ACNF composites. Interestingly, the CH4 adsorption of all ACNF samples obeyed the pseudo-second-order kinetic model at low pressure (4 bar), indicating the chemisorption behaviors. However, it obeyed the pseudo-first order at higher pressures (8 and 12 bar), indicating the physisorption behaviors. These results correspond to the textural properties that describe that the high adsorption capacity of CH4 at high pressure is mainly dependent upon the specific surface area (SSA), pore size distribution, and the suitable range of pore size.
    MeSH terms: Adsorption; Charcoal; Dietary Fiber; Food Hypersensitivity; Graphite; Kinetics; Oryza; Spectroscopy, Fourier Transform Infrared; Physical Phenomena; Nanofibers
  13. Salleh WMNHW, Shakri NM, Khamis S, Setzer WN, Nadri MH
    Nat Prod Res, 2020 Sep 14.
    PMID: 32927975 DOI: 10.1080/14786419.2020.1819274
    This study aims to assess the chemical compositions of the essential oils from three Horsfieldia species namely H. fulva Warb., H. sucosa Warb. and H. superba Warb., which are found in Malaysia. The essential oils were derived from the samples through hydrodistillation which were then characterised by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Based on the findings, the H. fulva, H. sucosa and H. superba essential oils represented 98.2%, 98.7% and 98.5% of the total oils, respectively. The major component of H. fulva oil was identified to be germacrene D (20.8%), H. sucosa oil mainly contained α-cadinol (17.5%), whereas H. superba oil was rich in δ-cadinene (18.2%). To the best of our knowledge, this is the first study of the composition of the essential oils from these selected Horsfieldia species.
    MeSH terms: Malaysia; Gas Chromatography-Mass Spectrometry; Oils, Volatile; Terpenes; Myristicaceae; Sesquiterpenes, Germacrane
  14. Lou J, Kc S, Toh KY, Dabak S, Adler A, Ahn J, et al.
    Int J Technol Assess Health Care, 2020 Oct;36(5):474-480.
    PMID: 32928330 DOI: 10.1017/S0266462320000628
    There is growing interest globally in using real-world data (RWD) and real-world evidence (RWE) for health technology assessment (HTA). Optimal collection, analysis, and use of RWD/RWE to inform HTA requires a conceptual framework to standardize processes and ensure consistency. However, such framework is currently lacking in Asia, a region that is likely to benefit from RWD/RWE for at least two reasons. First, there is often limited Asian representation in clinical trials unless specifically conducted in Asian populations, and RWD may help to fill the evidence gap. Second, in a few Asian health systems, reimbursement decisions are not made at market entry; thus, allowing RWD/RWE to be collected to give more certainty about the effectiveness of technologies in the local setting and inform their appropriate use. Furthermore, an alignment of RWD/RWE policies across Asia would equip decision makers with context-relevant evidence, and improve timely patient access to new technologies. Using data collected from eleven health systems in Asia, this paper provides a review of the current landscape of RWD/RWE in Asia to inform HTA and explores a way forward to align policies within the region. This paper concludes with a proposal to establish an international collaboration among academics and HTA agencies in the region: the REAL World Data In ASia for HEalth Technology Assessment in Reimbursement (REALISE) working group, which seeks to develop a non-binding guidance document on the use of RWD/RWE to inform HTA for decision making in Asia.
    MeSH terms: Data Accuracy; Asia; Cost-Benefit Analysis; Decision Making; Surveys and Questionnaires; Reimbursement Mechanisms*; Technology Assessment, Biomedical*; Telecommunications; Evidence-Based Medicine*
  15. Sheikhzadeh E, Eissa S, Ismail A, Zourob M
    Talanta, 2020 Dec 01;220:121392.
    PMID: 32928412 DOI: 10.1016/j.talanta.2020.121392
    COVID-19 pandemic is a serious global health issue today due to the rapid human to human transmission of SARS-CoV-2, a new type of coronavirus that causes fatal pneumonia. SARS -CoV-2 has a faster rate of transmission than other coronaviruses such as SARS and MERS and until now there are no approved specific drugs or vaccines for treatment. Thus, early diagnosis is crucial to prevent the extensive spread of the disease. The reverse transcription-polymerase chain reaction (RT-PCR) is the most routinely used method until now to detect SARS-CoV-2 infections. However, several other faster and accurate assays are being developed for the diagnosis of COVID-19 aiming to control the spread of infection through the identification of patients and immediate isolation. In this review, we will discuss the various detection methods of the SARS-CoV-2 virus including the recent developments in immunological assays, amplification techniques as well as biosensors.
    MeSH terms: Humans; Immunoassay; Pneumonia, Viral/diagnosis*; Biosensing Techniques; Polymerase Chain Reaction; Coronavirus Infections/diagnosis*; Clinical Laboratory Techniques/methods*; Early Diagnosis; Pandemics
  16. Nicholas AF, Hussein MZ, Zainal Z, Khadiran T
    Sci Rep, 2020 Sep 14;10(1):15047.
    PMID: 32929140 DOI: 10.1038/s41598-020-72019-1
    The effect of the surface area of palm kernel shell activated carbon (PKSAC) on the properties of n-octadecane-encapsulated shape stabilized phase change material (SSPCM) for thermal energy storage (TES) application were studied. Various surface areas of the PKSAC were prepared using different amounts of H3PO4 treatment given to palm kernel shells from 0, 5, 10, 30 and 40% before the activation. The impregnation of n-octadecane into the different surface areas of PKSACs produced SSPCMs with different physico-chemical characteristics. The DSC analysis indicates that the higher the surface area of the PKSAC resulted in the higher freezing temperature due to the higher PCM loading that was encapsulated into the PKSAC pores. The results obtained from XRD, FESEM, Raman spectroscopy, TGA/DTG and leakage study indicate that the PKSAC is a good framework material for the development of n-octadecane-encapsulated SSPCM. It was also found that the surface area and porosity of the frameworks, activated carbon play an important role on the PCM loading percentage and their ability to be used as a thermal energy storage material.
  17. Wee SL, Clarke AR
    Sci Rep, 2020 09 14;10(1):15004.
    PMID: 32929156 DOI: 10.1038/s41598-020-72209-x
    Males of certain Dacini fruit flies are strongly attracted to, and feed upon, plant secondary compounds such as methyl eugenol, raspberry ketone and zingerone. The consumed lure is generally found to induce physiological and behavioural changes that enhance the mating performance of lure-fed males. Male Bactrocera jarvisi respond strongly to zingerone from a young age, but only weakly respond to raspberry ketone. We hypothesized that this selective lure-response would be reflected in the physiological importance of the lure to the fly. We found that zingerone feeding by young males resulted in significantly greater mating success in competitive mating trials with lure-deprived flies, but the mating advantage was lost in older males. Lure dosage had a significant effect on the duration of the mating advantage, for example when fed 20 µg of zingerone, the advantage lasted only 1 day post-feeding, but when fed of 50 µg zingerone the advantage lasted 7 days. Raspberry ketone feeding did not confer any mating advantage to males except at one dosage (50 µg) for 1 day after feeding. When given a choice, B. jarvisi females preferred to mate with zingerone-fed versus to raspberry ketone-fed males. This study revealed lure, dosage and age of fly at time of lure administration are all important factors for maximising lure-enhanced fruit fly mating performance. These findings contribute to a better theoretical understanding of the evolution of fruit fly-lure interactions and may help improve fruit fly pest management via the Sterile Insect Technique through semiochemical-mediated enhancement of sterile male mating performance.
    MeSH terms: Age Factors; Animals; Butanones/administration & dosage; Butanones/pharmacology; Feeding Behavior*; Female; Guaiacol/administration & dosage; Guaiacol/analogs & derivatives; Guaiacol/pharmacology; Male; Sexual Behavior, Animal*; Tephritidae/physiology*
  18. Mousavi SM, Low FW, Hashemi SA, Lai CW, Ghasemi Y, Soroshnia S, et al.
    Artif Cells Nanomed Biotechnol, 2020 Dec;48(1):1189-1205.
    PMID: 32930615 DOI: 10.1080/21691401.2020.1817052
    Graphene and its derivative materials present high potential towards medical and biological applications, including drug delivery and bioimaging, due to their exceptional properties such as thermal conductivity and high specific surface area. The main focus of this work is to review the current development of graphene materials and the derivatives for biocompatible, bioimaging and drug delivery applications. Also, the synthesis methods with variation of graphene nanocomposites and the functionalisation will be further explained. For the graphene approaches, chemical vapour deposition (CVD) is the best-known technique to make high-quality graphene sheet by growth route with mass production. By considering the organic graphene nanocomposites, the biocompatibility and cytotoxic effects against graphene nanocomposites were evaluated for biomedical employments such as high quality bioimaging and effective drug delivery for cancer treatments. For example, graphene oxide incorporated with PEG and loaded with SN 38 for camptothecin analolgue as anticancer drug and revealed high cytotoxicity has an effect of 1000 times better effect than CPT in HCT-116 cells. Their drug delivery ability for both in-vivo and in-vitro applications compared to the controlled drugs such as doxorubicin (DOX) will be discussed accordingly. The graphene and its deriavatives possess some intriguing properties, which will lead to drug delivery due to strong biocompatibility and cyctotoxic effect towards biomedicine applications.
    MeSH terms: Animals; Graphite/chemistry*; Humans; Nanomedicine/methods*; Nanocomposites/chemistry*
  19. Wang YH, Bychkov A, Chakrabarti I, Jain D, Liu Z, He S, et al.
    Cancer Cytopathol, 2020 Dec;128(12):895-904.
    PMID: 32931161 DOI: 10.1002/cncy.22354
    BACKGROUND: The purpose of the current study was to examine the impact of coronavirus disease 2019 (COVID-19) on various aspects of cytology practice in the Asia-Pacific region.

    METHODS: An online questionnaire was distributed to cytopathology laboratories in 24 Asia-Pacific countries to explore the impact of restrictive measures on access to health care, use of general and personal protective equipment (PPE), and changes in cytology workflow and workload from February to April 2020.

    RESULTS: A total of 167 cytopathology laboratories from 24 countries responded to the survey; the majority reported that restrictive measures that limited the accessibility of health care services had been implemented in their cities and/or countries (80.8%) and their hospitals (83.8%). The respondents noted that COVID-19 had an impact on the cytologic workflow as well as the workload. Approximately one-half of the participants reported the implementation of new biosafety protocols (54.5%) as well as improvements in laboratory facilities (47.3%). Rearrangement or redeployment of the workforce was reported in 53.3% and 34.1% of laboratories, respectively. The majority of the respondents reported a significant reduction (>10%) in caseload associated with both gynecological (82.0%) and nongynecological specimens (78.4%). Most laboratories reported no significant change in the malignancy rates of both gynecological (67.7%) and nongynecological specimens (58.7%) compared with the same period in 2019.

    CONCLUSIONS: The results of the survey demonstrated that the COVID-19 pandemic resulted in a significant reduction in the number of cytology specimens examined along with the need to implement new biosafety protocols. These findings underscore the need for the worldwide standardization of biosafety protocols and cytology practice.

    MeSH terms: Personal Protective Equipment/standards; Asia; Communicable Disease Control/instrumentation; Communicable Disease Control/standards*; Health Manpower/organization & administration; Health Manpower/standards; Health Manpower/statistics & numerical data; Humans; Laboratories, Hospital/organization & administration*; Laboratories, Hospital/standards; Laboratories, Hospital/statistics & numerical data; Pathology, Clinical/organization & administration*; Pathology, Clinical/standards; Pathology, Clinical/statistics & numerical data; Workload/statistics & numerical data; Infectious Disease Transmission, Patient-to-Professional/prevention & control*; Pacific States; Pandemics/prevention & control
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