Browse publications by year: 2020

  1. Arisht SN, Abdul PM, Jasni J, Mohd Yasin NH, Lin SK, Wu SY, et al.
    Ecotoxicol Environ Saf, 2020 Oct 15;203:110991.
    PMID: 32888602 DOI: 10.1016/j.ecoenv.2020.110991
    The stimulant and toxicity effects of reported organic (acetic acid, propionic acid, butyric acid, formic acid, oil & grease) and inorganic (copper) by-products presented in palm oil mill effluent on anaerobic bacterial population were examined in this paper. The toxicity test had shown that acetic, propionic and butyric acids tend to stimulate the bacterial density level (survival rate more than 50%), while formic acid, copper, oil and grease were shown to have suppressed the density level (survival rate less than 50%). The highest biomass recorded was 1.66 mg/L for the concentration of acetic acid at 216 mg/L and lowest biomass concentration, 0.90 mg/L for copper at 1.40 mg/L. Biohydrogen-producing bacteria have a favourable growth rate around pH 5.5. The comparison of half maximal effective concentration (EC50) values between two test duration on the effects of organic and inorganic by-products postulate that bacteria had a higher tolerance towards volatile fatty acids. While acetic, butyric and propionic acids had exhibited higher tolerance EC50 values for bacteria, but the opposite trend was observed for formic acid, copper and oil & grease.
    MeSH terms: Bacteria, Anaerobic/drug effects*; Dose-Response Relationship, Drug; Hydrogen/metabolism; Industrial Waste/analysis; Toxicity Tests/methods*
  2. Tan YJ, Lim SY, Yong VW, Choo XY, Ng YD, Sugumaran K, et al.
    J Clin Densitom, 2020 07 30;24(3):351-361.
    PMID: 32888777 DOI: 10.1016/j.jocd.2020.07.001
    Osteoporotic fractures are common in Parkinson's disease (PD). Standard dual-energy X-ray absorptiometry (DXA) measuring bone mineral density (BMD) at the femoral neck and lumbar spine (central sites) has suboptimal sensitivity in predicting fracture risk in the general population. An association between sarcopenia and osteoporosis in PD has not been studied. We compared BMD and osteoporosis prevalence in PD patients vs controls; determined the osteoporosis detection rates using central alone vs central plus distal radius DXA; and analyzed factors (in particular, sarcopenia) associated with osteoporosis. One hundred and fifty-six subjects (102 patients with PD, 54 spousal/sibling controls) underwent femoral neck-lumbar spine-distal radius DXA. Seventy-three patients and 46 controls were assessed for sarcopenia using whole-body DXA and handgrip strength. Patients underwent clinical and serum biochemical evaluations. PD patients had significantly lower body mass index compared to controls. After adjustment for possible confounders, distal radius BMD and T-scores were significantly lower in PD patients compared to controls, but not at the femoral neck/lumbar spine. With distal radius DXA, an additional 11.0% of patients were diagnosed with osteoporosis (32.0% to 43.0%), vs 3.7% in controls (33.3% to 37.0%) additionally diagnosed; this increase was largely driven by the markedly higher detection rate in female PD patients. Female gender (adjusted odds ratio [ORadjusted] = 11.3, 95% confidence interval [CI]: 2.6-48.6) and sarcopenia (ORadjusted = 8.4, 95% CI: 1.1-64.9) were independent predictors for osteoporosis in PD. Distal radius DXA increased osteoporosis detection, especially in female PD patients, suggesting that diagnostic protocols for osteoporosis in PD could be optimized. A close association between osteoporosis and sarcopenia was documented for the first time in PD, which has important implications for clinical management and future research.
    MeSH terms: Female; Humans; Radius; Absorptiometry, Photon; Bone Density; Hand Strength
  3. Lee JM, Yek SH, Wilson RF, Rahman S
    Acta Trop, 2020 Dec;212:105683.
    PMID: 32888935 DOI: 10.1016/j.actatropica.2020.105683
    Understanding the diversity and dynamics of the microbiota within the mosquito holobiome is of great importance to apprehend how the microbiota modulates various complex processes and interactions. This study examined the bacterial composition of Aedes albopictus across land use type and mosquito sex in the state of Selangor, Malaysia using 16S rRNA sequencing. The bacterial community structure in mosquitoes was found to be influenced by land use type and mosquito sex, with the environment and mosquito diet respectively identified to be the most likely sources of microbes. We found that approximately 70% of the microbiota samples were dominated by Wolbachia and removing Wolbachia from analyses revealed the relatively even composition of the remaining bacterial microbiota. Furthermore, microbial interaction network analysis highlighted the prevalence of co-exclusionary patterns in all networks regardless of land use and mosquito sex, with Wolbachia exhibiting co-exclusionary interactions with other residential bacteria such as Xanthomonas, Xenophilus and Zymobacter.
    MeSH terms: Aedes/microbiology*; Animals; Bacteria/isolation & purification*; Female; Malaysia; Male; Wolbachia/isolation & purification; Microbial Interactions; Microbiota*
  4. Lim HJ, Saha T, Tey BT, Tan WS, Ooi CW
    Biosens Bioelectron, 2020 Nov 15;168:112513.
    PMID: 32889395 DOI: 10.1016/j.bios.2020.112513
    Infectious diseases are the ever-present threats to public health and the global economy. Accurate and timely diagnosis is crucial to impede the progression of a disease and break the chain of transmission. Conventional diagnostic techniques are typically time-consuming and costly, making them inefficient for early diagnosis of infections and inconvenient for use at the point of care. Developments of sensitive, rapid, and affordable diagnostic methods are necessary to improve the clinical management of infectious diseases. Quartz crystal microbalance (QCM) systems have emerged as a robust biosensing platform due to their label-free mechanism, which allows the detection and quantification of a wide range of biomolecules. The high sensitivity and short detection time offered by QCM-based biosensors are attractive for the early detection of infections and the routine monitoring of disease progression. Herein, the strategies employed in QCM-based biosensors for the detection of infectious diseases are extensively reviewed, with a focus on prevalent diseases for which improved diagnostic techniques are in high demand. The challenges to the clinical application of QCM-based biosensors are highlighted, along with an outline of the future scope of research in QCM-based diagnostics.
    MeSH terms: Humans; Biosensing Techniques*; Point-of-Care Systems; Quartz Crystal Microbalance Techniques
  5. Wong JJM, Lee SW, Lee JH
    Pediatr Crit Care Med, 2020 09;21(9):855-856.
    PMID: 32890097 DOI: 10.1097/PCC.0000000000002446
    MeSH terms: Child; Dyspnea; Humans; Respiration, Artificial; Respiratory Distress Syndrome, Adult*
  6. Mohd Umbaik NA, Mohamad I, Nik Hassan NFH
    J Craniofac Surg, 2020 10;31(7):2064-2065.
    PMID: 32890162 DOI: 10.1097/SCS.0000000000006839
    MeSH terms: Humans; Intubation, Intratracheal/adverse effects; Laryngoscopy; Vocal Cord Paralysis*; Vocal Cords
  7. Mirza MU, Saadabadi A, Vanmeert M, Salo-Ahen OMH, Abdullah I, Claes S, et al.
    Eur J Pharm Sci, 2020 Dec 01;155:105537.
    PMID: 32890663 DOI: 10.1016/j.ejps.2020.105537
    Chemokine receptors are key regulators of cell migration in terms of immunity and inflammation. Among these, CCR5 and CXCR4 play pivotal roles in cancer metastasis and HIV-1 transmission and infection. They act as essential co-receptors for HIV and furnish a route to the cell entry. In particular, inhibition of either CCR5 or CXCR4 leads very often the virus to shift to a more virulent dual-tropic strain. Therefore, dual receptor inhibition might improve the therapeutic strategies against HIV. In this study, we aimed to discover selective CCR5, CXCR4, and dual CCR5/CXCR4 antagonists using both receptor- and ligand-based computational methods. We employed this approach to fully incorporate the interaction attributes of the binding pocket together with molecular dynamics (MD) simulations and binding free energy calculations. The best hits were evaluated for their anti-HIV-1 activity against CXCR4- and CCR5-specific NL4.3 and BaL strains. Moreover, the Ca2+ mobilization assay was used to evaluate their antagonistic activity. From the 27 tested compounds, three were identified as inhibitors: compounds 27 (CCR5), 6 (CXCR4) and 3 (dual) with IC50 values ranging from 10.64 to 64.56 μM. The binding mode analysis suggests that the active compounds form a salt bridge with the glutamates and π-stacking interactions with the aromatic side chains binding site residues of the respective co-receptor. The presented hierarchical virtual screening approach provides essential aspects in identifying potential antagonists in terms of selectivity against a specific co-receptor. The compounds having multiple heterocyclic nitrogen atoms proved to be relatively more specific towards CXCR4 inhibition as compared to CCR5. The identified compounds serve as a starting point for further development of HIV entry inhibitors through synthesis and quantitative structure-activity relationship studies.
    MeSH terms: Binding Sites; Humans; Ligands; HIV-1*; Receptors, CCR5; HIV Fusion Inhibitors*; CCR5 Receptor Antagonists/pharmacology
  8. Raksasat R, Lim JW, Kiatkittipong W, Kiatkittipong K, Ho YC, Lam MK, et al.
    Environ Pollut, 2020 Dec;267:115488.
    PMID: 32891050 DOI: 10.1016/j.envpol.2020.115488
    The increase of annual organic wastes generated worldwide has become a major problem for many countries since the mismanagement could bring about negative effects on the environment besides, being costly for an innocuous disposal. Recently, insect larvae have been investigated to valorize organic wastes. This entomoremediation approach is rising from the ability of the insect larvae to convert organic wastes into its biomass via assimilation process as catapulted by the natural demand to complete its lifecycle. Among the insect species, black soldier fly or Hermetia illucens is widely researched since the larvae can grow in various environments while being saprophagous in nature. Even though black soldier fly larvae (BSFL) can ingest various decay materials, some organic wastes such as sewage sludge or lignocellulosic wastes such as waste coconut endosperm are destitute of decent nutrients that could retard the BSFL growth. Hence, blending with nutrient-rich low-cost substrates such as palm kernel expeller, soybean curd residue, etc. is employed to fortify the nutritional contents of larval feeding substrates prior to administering to the BSFL. Alternatively, microbial fermentation can be adopted to breakdown the lignocellulosic wastes, exuding essential nutrients for growing BSFL. Upon reaching maturity, the BSFL can be harvested to serve as the protein and lipid feedstock. The larval protein can be made into insect meal for farmed animals, whilst the lipid source could be extracted and transesterified into larval biodiesel to cushion the global energy demands. Henceforth, this review presents the influence of various organic wastes introduced to feed BSFL, targeting to reduce wastes and producing biochemicals from mature larvae through entomoremediation. Modification of recalcitrant organic wastes via fermentation processes is also unveiled to ameliorate the BSFL growth. Lastly, the sustainable applications of harvested BSFL biomass are as well covered together with the immediate shortcomings that entail further researches.
    MeSH terms: Animals; Diptera*; Larva; Lipids; Sewage; Simuliidae*; Biofuels
  9. Nayak SB, Soumya KV
    Surg Radiol Anat, 2020 06;42(6):717.
    PMID: 31984433 DOI: 10.1007/s00276-020-02418-6
    MeSH terms: Electromyography; Humans; Neck Muscles*
  10. Kraft TS, Venkataraman VV, Endicott KL, Endicott KM
    Evol. Anthropol., 2020 May;29(3):117-124.
    PMID: 32472595 DOI: 10.1002/evan.21835
    The human evolutionary sciences place high value on quantitative data from traditional small-scale societies that are rapidly modernizing. These data often stem from the sustained ethnographic work of anthropologists who are today nearing the end of their careers. Yet many quantitative ethnographic data are preserved only in summary formats that do not reflect the rich and variable ethnographic reality often described in unpublished field notes, nor the deep knowledge of their collectors. In raw disaggregated formats, such data have tremendous scientific value when used in conjunction with modern statistical techniques and as part of comparative analyses. Through a personal example of longitudinal research with Batek hunter-gatherers that involved collaboration across generations of researchers, we argue that quantifiable ethnographic records, just like material artifacts, deserve high-priority preservation efforts. We discuss the benefits, challenges, and possible avenues forward for digitizing, preserving, and archiving ethnographic data before it is too late.
    MeSH terms: Anthropology, Cultural/instrumentation; Anthropology, Cultural/standards*; Humans; Malaysia; Records as Topic/standards*; Records as Topic/statistics & numerical data
  11. Prashanti E, Ramnarayan K
    Adv Physiol Educ, 2020 Dec 01;44(4):550-553.
    PMID: 32880485 DOI: 10.1152/advan.00085.2020
    To foster a milieu in which student learning can be optimum, teachers need to be aware of the attributes of a safe learning environment. This is the space created in the students' minds to seamlessly promote learning. The 10 maxims, presented in this paper, are the cornerstones, nay, the capstones, for making this happen.
    MeSH terms: Awareness; Humans; Learning; Students; Educational Personnel
  12. Chew N, Noor Azhar AM, Bustam A, Azanan MS, Wang C, Lum LCS
    PLoS Negl Trop Dis, 2020 09;14(9):e0008562.
    PMID: 32881914 DOI: 10.1371/journal.pntd.0008562
    BACKGROUND: Dengue is a systemic and dynamic disease with symptoms ranging from undifferentiated fever to dengue shock syndrome. Assessment of patients' severity of dehydration is integral to appropriate care and management. Urine colour has been shown to have a high correlation with overall assessment of hydration status. This study tests the feasibility of measuring dehydration severity in dengue fever patients by comparing urine colour captured by mobile phone cameras to established laboratory parameters.

    METHODOLOGY/PRINCIPAL FINDINGS: Photos of urine samples were taken in a customized photo booth, then processed using Adobe Photoshop to index urine colour into the red, green, and blue (RGB) colour space and assigned a unique RGB value. The RGB values were then correlated with patients' clinical and laboratory hydration indices using Pearson's correlation and multiple linear regression. There were strong correlations between urine osmolality and the RGB of urine colour, with r = -0.701 (red), r = -0.741 (green), and r = -0.761 (blue) (all p-value <0.05). There were strong correlations between urine specific gravity and the RGB of urine colour, with r = -0.759 (red), r = -0.785 (green), and r = -0.820 (blue) (all p-value <0.05). The blue component had the highest correlations with urine specific gravity and urine osmolality. There were moderate correlations between RGB components and serum urea, at r = -0.338 (red), -0.329 (green), -0.360 (blue). In terms of urine biochemical parameters linked to dehydration, multiple linear regression studies showed that the green colourimetry code was predictive of urine osmolality (β coefficient -0.082, p-value <0.001) while the blue colourimetry code was predictive of urine specific gravity (β coefficient -2,946.255, p-value 0.007).

    CONCLUSIONS/SIGNIFICANCE: Urine colourimetry using mobile phones was highly correlated with the hydration status of dengue patients, making it a potentially useful hydration status tool.

    MeSH terms: Adolescent; Adult; Child; Color; Colorimetry/instrumentation; Colorimetry/methods*; Cross-Sectional Studies; Dehydration/urine*; Dengue/pathology; Dengue/urine*; Female; Humans; Male; Middle Aged; Urine/chemistry*; Cell Phones*; Young Adult
  13. Muchtaridi M, Fauzi M, Khairul Ikram NK, Mohd Gazzali A, Wahab HA
    Molecules, 2020 Sep 01;25(17).
    PMID: 32882868 DOI: 10.3390/molecules25173980
    Over the years, coronaviruses (CoV) have posed a severe public health threat, causing an increase in mortality and morbidity rates throughout the world. The recent outbreak of a novel coronavirus, named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the current Coronavirus Disease 2019 (COVID-19) pandemic that affected more than 215 countries with over 23 million cases and 800,000 deaths as of today. The situation is critical, especially with the absence of specific medicines or vaccines; hence, efforts toward the development of anti-COVID-19 medicines are being intensively undertaken. One of the potential therapeutic targets of anti-COVID-19 drugs is the angiotensin-converting enzyme 2 (ACE2). ACE2 was identified as a key functional receptor for CoV associated with COVID-19. ACE2, which is located on the surface of the host cells, binds effectively to the spike protein of CoV, thus enabling the virus to infect the epithelial cells of the host. Previous studies showed that certain flavonoids exhibit angiotensin-converting enzyme inhibition activity, which plays a crucial role in the regulation of arterial blood pressure. Thus, it is being postulated that these flavonoids might also interact with ACE2. This postulation might be of interest because these compounds also show antiviral activity in vitro. This article summarizes the natural flavonoids with potential efficacy against COVID-19 through ACE2 receptor inhibition.
    MeSH terms: Angiotensin-Converting Enzyme Inhibitors/pharmacology*; Angiotensin-Converting Enzyme Inhibitors/chemistry; Antiviral Agents/pharmacology*; Antiviral Agents/chemistry; Biological Products/pharmacology*; Biological Products/chemistry; Disease Susceptibility; Flavonoids/pharmacology*; Flavonoids/chemistry; Humans; Peptidyl-Dipeptidase A/metabolism; Peptidyl-Dipeptidase A/chemistry; Life Cycle Stages; Models, Molecular; Pneumonia, Viral/drug therapy; Pneumonia, Viral/epidemiology; Pneumonia, Viral/virology*; Population Surveillance; Structure-Activity Relationship; Coronavirus Infections/drug therapy; Coronavirus Infections/epidemiology; Coronavirus Infections/virology*; Pandemics
  14. Sukeri S, Zahiruddin WM, Shafei MN, Hamat RA, Osman M, Jamaluddin TZMT, et al.
    PMID: 32882876 DOI: 10.3390/ijerph17176362
    BACKGROUND: Perceived severity and susceptibility refers to one's belief of the seriousness and the risk of contracting a specific disease. It is an essential study in public health as it assists in the understanding of the motivating factors towards disease prevention. This qualitative study aimed to explore perceived severity and susceptibility towards leptospirosis infection among respondents in two states of Malaysia.

    METHODS: Focus group discussions using the phenomenology approach was conducted involving 72 respondents in Selangor and Kelantan. Data were examined using content analysis.

    RESULTS: Respondents perceived leptospirosis infection as severe due to its poor disease prognosis and complications. However, some rated it less severe when compared with other chronic diseases such as cancer and AIDS. Their perceptions were influenced by their knowledge about the disease, media portrayal and frequency of health campaigns by the government. All respondents believed they were not susceptible to the disease.

    CONCLUSION: The low perceived susceptibility of leptospirosis infection is a matter of concern as it may contribute to respondents' lack of motivation towards preventing the disease. The study findings may provide the basis for health promotional activities designed to heighten public perceived threat towards leptospirosis infection and thereby improving preventive health behaviors for avoiding leptospirosis.

    MeSH terms: Acquired Immunodeficiency Syndrome; Chronic Disease; Government; Health Promotion; Leptospirosis; Malaysia; Motivation; Neoplasms; Preventive Health Services; Prognosis; Public Health; Surveys and Questionnaires; Health Behavior; Focus Groups; Qualitative Research
  15. Fathoni I, Petitbois JG, Alarif WM, Abdel-Lateff A, Al-Lihaibi SS, Yoshimura E, et al.
    Molecules, 2020 Sep 01;25(17).
    PMID: 32882989 DOI: 10.3390/molecules25173986
    Cyanobacteria are reported as rich sources of secondary metabolites that provide biological activities such as enzyme inhibition and cytotoxicity. Ten depsipeptide derivatives (lyngbyabellins) were isolated from a Malaysian Moorea bouillonii and a Red Sea Okeania sp.: lyngbyabellins G (1), O (2), P (3), H (4), A (7), 27-deoxylyngbyabellin A (5), and homohydroxydolabellin (6). This study indicated that lyngbyabellins displayed cytotoxicity, antimalarial, and antifouling activities. The isolated compounds were tested for cytotoxic effect against human breast cancer cells (MCF7), for antifouling activity against Amphibalanus amphitrite barnacle larvae, and for antiplasmodial effect towards Plasmodium falciparum. Lyngbyabellins A and G displayed potent antiplasmodial effect against Plasmodium, whereas homohydroxydolabellin showed moderate effect. For antifouling activity, the side chain decreases the activity slightly, but the essential feature is the acyclic structure. As previously reported, the acyclic lyngbyabellins are less cytotoxic than the corresponding cyclic ones, and the side chain increases cytotoxicity. This study revealed that lyngbyabellins, despite being cytotoxic agents as previously reported, also exhibit antimalarial and antifouling activities. The unique chemical structures and functionalities of lyngbyabellin play an essential role in their biological activities.
    MeSH terms: Cyanobacteria/chemistry*; Antimalarials/pharmacology; Humans; Cell Death/drug effects; Depsipeptides/isolation & purification; Depsipeptides/pharmacology*; Depsipeptides/chemistry; Biofouling; MCF-7 Cells
  16. Rosli NA, Aziz HA, Selamat MR, Lim LLP
    J Environ Manage, 2020 Jun 01;263:110420.
    PMID: 32883483 DOI: 10.1016/j.jenvman.2020.110420
    This study proposed the recycling of sewage sludge (SS) and red gypsum (RG) as potential temporary landfill cover materials. Mixtures with different SS and RG compositions were prepared and tested in determining the most suitable design mix based on the resulting physical, mechanical, and geotechnical properties, namely the hydraulic conductivity, compressive strength, and plasticity. A maximum compressive strength of 524 kPa was achieved for the optimum SS:RG composition of 1:1, corresponding to Ca:Si composition of 2.5:1, which was appropriate to form the calcium silicate hydrate (CSH) gel. The SS and RG compositions did not affect the hydraulic conductivity, which was in the order 10-5 cm/s for all mixtures. Mixtures with RG greater than SS in composition exhibited plastic behaviour due to the Fe content in the RG, which helped minimize the risk of cracking. The optimum mixture had compressive strength greater than the specified minimum of 345 kPa, medium hydraulic conductivity, and moderate plasticity, thus appropriate for application as an alternative material for the temporary landfill cover in the tropics.
    MeSH terms: Calcium Sulfate*; Sewage*; Waste Disposal Facilities
  17. Pham TS, Reda A, Ngan Nguyen TT, Ng SJ, Huan VT, Viet DC, et al.
    Transfus Apher Sci, 2020 Dec;59(6):102907.
    PMID: 32883595 DOI: 10.1016/j.transci.2020.102907
    We report a case of blood exchange transfusion to treat acute liver failure following hepatitis B infection at the Infectious Disease Department of Children's Hospital No.2 in Ho Chi Minh City, Vietnam. A 3.5-month old baby boy was admitted to the hospital with a presentation of progressively worsening jaundice for the past one month. The patient was diagnosed with hepatitis B infection with a positive HBV DNA quantitative assay. Plasma exchange was indicated in view of progressive liver failure and gradually increasing hepatic coma. However, it was impossible to perform plasmapheresis in this case because the patient was small (in terms of age and weight) and there was no suitable plasma exchange filter. Accordingly, the patient was treated with 3 times of blood exchange transfusion in combination with an antiviral drug, lamivudine. After each blood exchange transfusion, the biochemical values (bilirubin, liver enzymes, and coagulation profile) gradually improved and he was discharged after 1 month of treatment. Blood exchange transfusion is an effective procedure for managing acute liver failure, where plasma exchange is not possible while waiting for the recovery of liver functions or liver transplantation.
    MeSH terms: Exchange Transfusion, Whole Blood/methods*; Hepatitis, Viral, Human/therapy*; Humans; Infant; Male; Liver Failure, Acute/therapy*
  18. Guo J, Sahai AV, Teoh A, Arcidiacono PG, Larghi A, Saftoiu A, et al.
    Endosc Ultrasound, 2020 9 5;9(5):319-328.
    PMID: 32883921 DOI: 10.4103/eus.eus_56_20
    Background and Objectives: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) and fine needle biopsy (FNB) are effective techniques that are widely used for tissue acquisition. However, it remains unclear how to obtain high-quality specimens. Therefore, we conducted a survey of EUS-FNA and FNB techniques to determine practice patterns worldwide and to develop strong recommendations based on the experience of experts in the field.

    Methods: This was a worldwide multi-institutional survey among members of the International Society of EUS Task Force (ISEUS-TF). The survey was administered by E-mail through the SurveyMonkey website. In some cases, percentage agreement with some statements was calculated; in others, the options with the greatest numbers of responses were summarized. Another questionnaire about the level of recommendation was designed to assess the respondents' answers.

    Results: ISEUS-TF members developed a questionnaire containing 17 questions that was sent to 53 experts. Thirty-five experts completed the survey within the specified period. Among them, 40% and 54.3% performed 50-200 and more than 200 EUS sampling procedures annually, respectively. Some practice patterns regarding FNA/FNB were recommended.

    Conclusion: This is the first worldwide survey of EUS-FNA and FNB practice patterns. The results showed wide variations in practice patterns. Randomized studies are urgently needed to establish the best approach for optimizing the FNA/FNB procedures.

    MeSH terms: Needles; Pancreatic Neoplasms; Surveys and Questionnaires; Advisory Committees; Electronic Mail; Endoscopic Ultrasound-Guided Fine Needle Aspiration
  19. Christakoudi S, Tsilidis KK, Muller DC, Freisling H, Weiderpass E, Overvad K, et al.
    Sci Rep, 2020 Sep 03;10(1):14541.
    PMID: 32883969 DOI: 10.1038/s41598-020-71302-5
    Abdominal and general adiposity are independently associated with mortality, but there is no consensus on how best to assess abdominal adiposity. We compared the ability of alternative waist indices to complement body mass index (BMI) when assessing all-cause mortality. We used data from 352,985 participants in the European Prospective Investigation into Cancer and Nutrition (EPIC) and Cox proportional hazards models adjusted for other risk factors. During a mean follow-up of 16.1 years, 38,178 participants died. Combining in one model BMI and a strongly correlated waist index altered the association patterns with mortality, to a predominantly negative association for BMI and a stronger positive association for the waist index, while combining BMI with the uncorrelated A Body Shape Index (ABSI) preserved the association patterns. Sex-specific cohort-wide quartiles of waist indices correlated with BMI could not separate high-risk from low-risk individuals within underweight (BMI 
    MeSH terms: Europe; Female; Humans; Male; Risk Factors; Cohort Studies; Body Mass Index; Proportional Hazards Models; Waist-Hip Ratio; Waist Circumference/physiology; Obesity, Abdominal/mortality*
  20. Rudolph TB, Ruckelshaus M, Swilling M, Allison EH, Österblom H, Gelcich S, et al.
    Nat Commun, 2020 09 03;11(1):4494.
    PMID: 32883972 DOI: 10.1038/s41467-020-18409-5
    An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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