Browse publications by year: 2021

  1. Castagna C, Leguizamón AP, Póvoas SC
    PMID: 34156179 DOI: 10.23736/S0022-4707.21.12293-5
    BACKGROUND: This study aim was to profile physical fitness in talented football referees (FR) with performance relevant field-tests across time.

    METHODS: Thirty-eight male FR (age 28±1.5 years, height 178±5.1 cm, body mass 69.0±7.34 kg, body fat 17.2±2.87%) were observed for 15 months. Endurance was assessed with the Yo-Yo Intermittent Recovery Test level 1 (YYIR1) and repeated sprint ability (RSA) with 5x30m line-sprints with 30s recovery (5x30m). Long sprint endurance (LSA) and change of direction ability (COD) evaluated with novel field-tests. FR was tested six times (every three months) during the study.

    RESULTS: YYIR1 performance showed large increments across testing occasions. Sprint time in the COD largely and significantly decreased across the testing occasions. Very large associations were reported between 5x30m and LSA tests grand mean (r=0.89, 0.78-0.94, P<0.0001). A nearly perfect (r=0.97, 0.94-0.99, P<0.0001) association was observed between 5x30m best sprint and 5x30m grand means.

    CONCLUSIONS: The results of this study revealed ability-related variations in performance across time. The effect of training and competitions on the determinism of physical fitness in refereeing seem plausible. Interestingly, sprint endurance tests shared a very large variance proposing tests interchangeability and mutual physiological demands. This study information provides useful information for the development of sound field-tests batteries in talented FR.

  2. Sweet M, Villela H, Keller-Costa T, Costa R, Romano S, Bourne DG, et al.
    mSystems, 2021 Jun 29;6(3):e0124920.
    PMID: 34156291 DOI: 10.1128/mSystems.01249-20
    Bacteria associated with coral hosts are diverse and abundant, with recent studies suggesting involvement of these symbionts in host resilience to anthropogenic stress. Despite their putative importance, the work dedicated to culturing coral-associated bacteria has received little attention. Combining published and unpublished data, here we report a comprehensive overview of the diversity and function of culturable bacteria isolated from corals originating from tropical, temperate, and cold-water habitats. A total of 3,055 isolates from 52 studies were considered by our metasurvey. Of these, 1,045 had full-length 16S rRNA gene sequences, spanning 138 formally described and 12 putatively novel bacterial genera across the Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria phyla. We performed comparative genomic analysis using the available genomes of 74 strains and identified potential signatures of beneficial bacterium-coral symbioses among the strains. Our analysis revealed >400 biosynthetic gene clusters that underlie the biosynthesis of antioxidant, antimicrobial, cytotoxic, and other secondary metabolites. Moreover, we uncovered genomic features-not previously described for coral-bacterium symbioses-potentially involved in host colonization and host-symbiont recognition, antiviral defense mechanisms, and/or integrated metabolic interactions, which we suggest as novel targets for the screening of coral probiotics. Our results highlight the importance of bacterial cultures to elucidate coral holobiont functioning and guide the selection of probiotic candidates to promote coral resilience and improve holistic and customized reef restoration and rehabilitation efforts. IMPORTANCE Our paper is the first study to synthesize currently available but decentralized data of cultured microbes associated with corals. We were able to collate 3,055 isolates across a number of published studies and unpublished collections from various laboratories and researchers around the world. This equated to 1,045 individual isolates which had full-length 16S rRNA gene sequences, after filtering of the original 3,055. We also explored which of these had genomes available. Originally, only 36 were available, and as part of this study, we added a further 38-equating to 74 in total. From this, we investigated potential genetic signatures that may facilitate a host-associated lifestyle. Further, such a resource is an important step in the selection of probiotic candidates, which are being investigated for promoting coral resilience and potentially applied as a novel strategy in reef restoration and rehabilitation efforts. In the spirit of open access, we have ensured this collection is available to the wider research community through the web site http://isolates.reefgenomics.org/ with the hope many scientists across the globe will ask for access to these cultures for future studies.
  3. Shakhih MFM, Ridzuan N, Wahab AA, Zainuddin NF, Delestri LFU, Rosslan AS, et al.
    Med Biol Eng Comput, 2021 Aug;59(7-8):1447-1459.
    PMID: 34156602 DOI: 10.1007/s11517-021-02387-x
    Surface electromyography (sEMG) has been widely used in evaluating muscle fatigue among athletes where electrodes are attached on the skin during the activity. Recently, infrared thermography technique (IRT) has gain popularity and shown to be another preferred method in monitoring and predicting muscle fatigue non-obstructively. This paper investigates the correlation between surface temperature and muscle activation parameters obtained using both IRT and sEMG methods simultaneously. Twenty healthy subjects were required to perform a repetitive calf raise exercise with various loads attached around their ankle for 3 min to induce fatigue on the targeted gastrocnemius muscles. Average temperature and temperature difference information were extracted from thermal images, while root mean square (RMS) and median frequency (MF) were extracted from sEMG signals. Spearman statistical analysis performed shows that there is a significant correlation between average temperature with RMS and between temperature difference with MF values at p<0.05. While ANOVA test conducted shows that there is significant impact of loads on RMS and MF where F=12.61 and 3.59, respectively, at p< 0.05. This study suggested that skin surface temperature can be utilized in monitoring and predicting muscle fatigue in low intensity dynamic exercise and can be extended to other dynamic exercises.
    MeSH terms: Electromyography; Humans; Thermography*; Exercise; Muscle, Skeletal; Muscle Fatigue*
  4. Yatim ANM, Latif MT, Sofwan NM, Ahamad F, Khan MF, Mahiyuddin WRW, et al.
    Environ Sci Pollut Res Int, 2021 Nov;28(42):60209-60220.
    PMID: 34156627 DOI: 10.1007/s11356-021-14962-8
    This study aims to examine the relationship between daily temperature and mortality in the Klang Valley, Malaysia, over the period 2006-2015. A quasi-Poisson generalized linear model combined with a distributed lag non-linear model (DLNM) was used to estimate the association between the mean temperature and mortality categories (natural n=69,542, cardiovascular n= 15,581, and respiratory disease n=10,119). Particulate matter with an aerodynamic diameter below 10 μm (PM10) and surface ozone (O3) was adjusted as a potential confounding factor. The relative risk (RR) of natural mortality associated with extreme cold temperature (1st percentile of temperature, 25.2 °C) over lags 0-28 days was 1.26 (95% confidence interval (CI): 1.00, 1.60), compared with the minimum mortality temperature (28.2 °C). The relative risk associated with extremely hot temperature (99th percentile of temperature, 30.2 °C) over lags 0-3 days was 1.09 (95% CI: 1.02, 1.17). Heat effects were immediate whereas cold effects were delayed and lasted longer. People with respiratory diseases, the elderly, and women were the most vulnerable groups when it came to the effects of extremely high temperatures. Extreme temperatures did not dramatically change the temperature-mortality risk estimates made before and after adjustments for air pollutant (PM10 and O3) levels.
  5. Tan GJ, Tan MP, Luben RN, Wareham NJ, Khaw KT, Myint PK
    Geriatr Gerontol Int, 2021 Aug;21(8):657-663.
    PMID: 34156750 DOI: 10.1111/ggi.14219
    AIM: To evaluate the relationship between habitual alcohol consumption and the risk of falls hospitalization.

    METHODS: The EPIC-Norfolk is a prospective population-based cohort study in Norfolk, UK. In total, 25 637 community dwelling adults aged 40-79 years were recruited. Units of alcohol consumed per week were measured using a validated Food Frequency Questionnaire. The main outcome was the first hospital admission following a fall.

    RESULTS: Over a median follow-up period of 11.5 years (299 211 total person years), the cumulative incidence function (95% confidence interval) of hospitalized falls at 121-180 months for non-users, light (>0 to ≤7 units/week), moderate (>7 to ≤28 units/week) and heavy (>28 units/week) were 11.08 (9.94-12.35), 7.53 (7.02-8.08), 5.91 (5.29-6.59) and 8.20 (6.35-10.56), respectively. Moderate alcohol consumption was independently associated with a reduced risk of falls hospitalization after adjustment for most major confounders (hazard ratio = 0.88; 95% confidence interval 0.79-0.99). The relationship between light alcohol consumption and falls hospitalization was attenuated by gender differences. Alcohol intake higher than the recommended threshold of 28 units/week was associated with an increased risk of falls hospitalization (hazard ratio 1.40 [1.14-1.73]).

    CONCLUSIONS: Moderate alcohol consumption appears to be associated with a reduced risk of falls hospitalization, and intake above the recommended limit is associated with an increased risk. This provides incentive to limit alcohol consumption within the recommended range and has important implications for public health policies for aging populations. Geriatr Gerontol Int 2021; 21: 657-663.

    MeSH terms: Accidental Falls*; Hospitalization; Humans; Prospective Studies; Risk Factors; Cohort Studies
  6. Masngut N, Mohamad E
    J Med Internet Res, 2021 08 04;23(8):e28074.
    PMID: 34156967 DOI: 10.2196/28074
    BACKGROUND: The COVID-19 health crisis has posed an unprecedented challenge for governments worldwide to manage and communicate about the pandemic effectively, while maintaining public trust. Good leadership image in times of a health emergency is paramount to ensure public confidence in governments' abilities to manage the crisis.

    OBJECTIVE: The aim of this study was to identify types of image repair strategies utilized by the Malaysian government in their communication about COVID-19 in the media and analyze public responses to these messages on social media.

    METHODS: Content analysis was employed to analyze 120 media statements and 382 comments retrieved from Facebook pages of 2 mainstream newspapers-Berita Harian and The Star. These media statements and comments were collected within a span of 6 weeks prior to and during the first implementation of Movement Control Order by the Malaysian Government. The media statements were analyzed according to Image Repair Theory to categorize strategies employed in government communications related to COVID-19 crisis. Public opinion responses were measured using modified lexicon-based sentiment analysis to categorize positive, negative, and neutral statements.

    RESULTS: The Malaysian government employed all 5 Image Repair Theory strategies in their communications in both newspapers. The strategy most utilized was reducing offensiveness (75/120, 62.5%), followed by corrective action (30/120, 25.0%), evading responsibilities (10/120, 8.3%), denial (4/120, 3.3%), and mortification (1/120, 0.8%). This study also found multiple substrategies in government media statements including denial, shifting blame, provocation, defeasibility, accident, good intention, bolstering, minimization, differentiation, transcendence, attacking accuser, resolve problem, prevent recurrence, admit wrongdoing, and apologize. This study also found that 64.7% of public opinion was positive in response to media statements made by the Malaysian government and also revealed a significant positive association (P=.04) between image repair strategies utilized by the Malaysian government and public opinion.

    CONCLUSIONS: Communication in the media may assist the government in fostering positive support from the public. Suitable image repair strategies could garner positive public responses and help build trust in times of crisis.

    MeSH terms: Communication; Government; Humans; Public Opinion; Social Media*
  7. Ikram M, Mahmood A, Haider A, Naz S, Ul-Hamid A, Nabgan W, et al.
    Int J Biol Macromol, 2021 Aug 31;185:153-164.
    PMID: 34157328 DOI: 10.1016/j.ijbiomac.2021.06.101
    Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation. The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product. Phase constitution, functional group analysis, optical behavior, elemental composition, morphology and microstructure were examined using XRD, FTIR, UV-Vis spectrophotometer, EDS and HR-TEM. Highly efficient photocatalytic performance was observed in basic medium (98%) relative to neutral (65%), and acidic (83%) was observed upon Mg and CNC co-doping. Significant bactericidal activity of doped ZnO nanoparticles depicted inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively. Overall, doped nanostructures showed significant (P 
    MeSH terms: Anti-Bacterial Agents/pharmacology*; Anti-Bacterial Agents/chemistry; Cellulose/chemistry*; Computer Simulation; Escherichia coli/drug effects; Magnesium/chemistry*; Methylene Blue/chemistry*; Microbial Sensitivity Tests; Chemical Precipitation; Staphylococcus aureus/drug effects; X-Ray Diffraction; Zinc Oxide/pharmacology*; Zinc Oxide/chemistry; Nanostructures; Photochemical Processes; Molecular Docking Simulation
  8. Ching JJ, Shuib AS, Abdullah N, Majid NA, Taufek NM, Sutra J, et al.
    Fish Shellfish Immunol, 2021 Sep;116:61-73.
    PMID: 34157396 DOI: 10.1016/j.fsi.2021.06.005
    In aquaculture, commercial fish such as red hybrid tilapia are usually raised at high density to boost the production within a short period of time. This overcrowded environment, however, may cause stress to the cultured fish and increase susceptibility to infectious diseases. Antibiotics and chemotherapeutics are used by fish farmers to overcome these challenges, but this may increase the production cost. Studies have reported on the potential of mushroom polysaccharides that can act as immunostimulants to enhance the immune response and disease resistance in fish. In the current study, hot water extract (HWE) from mushroom stalk waste (MSW) was used to formulate fish feed and hence administered to red hybrid tilapia to observe the activation of immune system. Upon 30 days of feeding, the fish were challenged with pathogen-associated molecular patterns (PAMPs) such as lipopolysaccharides (LPS) and polyinosinic:polycytidylic acid (poly (I:C)) to mimic bacterial and viral infection, respectively. HWE supplementation promoted better feed utilisation in red hybrid tilapia although it did not increase the body weight gain and specific growth rate compared to the control diet. The innate immunological parameters such as phagocytic activity and respiratory burst activity were significantly higher in HWE-supplemented group than that of the control group following PAMPs challenges. HWE-supplemented diet also resulted in higher mRNA transcription of il1b and tnfa in midgut, spleen and head kidney at 1-day post PAMPs injection. Tlr3 exhibited the highest upregulation in the HWE fed fish injected with poly (I:C). At 3-days post PAMPs injection, both ighm and tcrb expression were upregulated significantly in the spleen and head kidney. Results showed that HWE supplementation enhances the immune responses of red hybrid tilapia and induced a higher serum bactericidal activity against S. agalactiae.
    MeSH terms: Pathogen-Associated Molecular Pattern Molecules/pharmacology*; Animals; Animal Feed; Chimera; Gene Expression Regulation/drug effects; Gene Expression Regulation/immunology; Hot Temperature; Immunity, Innate/drug effects; Leukocytes/drug effects; Leukocytes/immunology; Lipopolysaccharides/pharmacology*; Phagocytosis/drug effects; Poly I-C/pharmacology*; Spleen/drug effects; Spleen/immunology; Streptococcus agalactiae/immunology; Tumor Necrosis Factor-alpha/genetics; Waste Products; Water; Dietary Supplements*; Pleurotus*; Complex Mixtures/pharmacology*; Interleukin-1beta/genetics; Head Kidney/drug effects; Head Kidney/immunology
  9. Volpiano CG, Sant'Anna FH, da Mota FF, Sangal V, Sutcliffe I, Munusamy M, et al.
    Syst Appl Microbiol, 2021 Jul;44(4):126223.
    PMID: 34157595 DOI: 10.1016/j.syapm.2021.126223
    Streptomyces thermoautotrophicus UBT1T has been suggested to merit generic status due to its phylogenetic placement and distinctive phenotypes among Actinomycetia. To evaluate whether 'S. thermoautotrophicus' represents a higher taxonomic rank, 'S. thermoautotrophicus' strains UBT1T and H1 were compared to Actinomycetia using 16S rRNA gene sequences and comparative genome analyses. The UBT1T and H1 genomes each contain at least two different 16S rRNA sequences, which are closely related to those of Acidothermus cellulolyticus (order Acidothermales). In multigene-based phylogenomic trees, UBT1T and H1 typically formed a sister group to the Streptosporangiales-Acidothermales clade. The Average Amino Acid Identity, Percentage of Conserved Proteins, and whole-genome Average Nucleotide Identity (Alignment Fraction) values were ≤58.5%, ≤48%, ≤75.5% (0.3) between 'S. thermoautotrophicus' and Streptosporangiales members, all below the respective thresholds for delineating genera. The values for genomics comparisons between strains UBT1T and H1 with Acidothermales, as well as members of the genus Streptomyces, were even lower. A review of the 'S. thermoautotrophicus' proteomic profiles and KEGG orthology demonstrated that UBT1T and H1 present pronounced differences, both tested and predicted, in phenotypic and chemotaxonomic characteristics compared to its sister clades and Streptomyces. The distinct phylogenetic position and the combination of genotypic and phenotypic characteristics justify the proposal of Carbonactinospora gen. nov., with the type species Carbonactinospora thermoautotrophica comb. nov. (type strain UBT1T, = DSM 100163T = KCTC 49540T) belonging to Carbonactinosporaceae fam. nov. within Actinomycetia.
  10. Hai A, Bharath G, Daud M, Rambabu K, Ali I, Hasan SW, et al.
    Chemosphere, 2021 Nov;283:131162.
    PMID: 34157626 DOI: 10.1016/j.chemosphere.2021.131162
    Pyrolysis of agricultural biomass is a promising technique for producing renewable energy and effectively managing solid waste. In this study, groundnut shell (GNS) was processed at 500 °C in an inert gas atmosphere with a gas flow rate and a heating rate of 10 mL/min and 10 °C/min, respectively, in a custom-designed fluidized bed pyrolytic-reactor. Under optimal operating conditions, the GNS-derived pyrolytic-oil yield was 62.8 wt.%, with the corresponding biochar (19.5 wt.%) and biogas yields (17.7 wt.%). The GC-MS analysis of the GNS-based bio-oil confirmed the presence of (trifluoromethyl)pyridin-2-amine (18.814%), 2-Fluoroformyl-3,3,4,4-tetrafluoro-1,2-oxazetidine (16.23%), 5,7-dimethyl-1H-Indazole (11.613%), N-methyl-N-nitropropan-2-amine (6.5%) and butyl piperidino sulfone (5.668%) as major components, which are used as building blocks in the biofuel, pharmaceutical, and food industries. Furthermore, a 2 × 5 × 1 artificial neural network (ANN) architecture was developed to predict the decomposition behavior of GNS at heating rates of 5, 10, and 20 °C/min, while the thermodynamic and kinetic parameters were estimated using a non-isothermal model-free method. The Popescu method predicted activation energy (Ea) of GNS biomass ranging from 111 kJ/mol to 260 kJ/mol, with changes in enthalpy (ΔH), Gibbs-free energy (ΔG), and entropy (ΔS) ranging from 106 to 254 kJ/mol, 162-241 kJ/mol, and -0.0937 to 0.0598 kJ/mol/K, respectively. The extraction of high-quality precursors from GNS pyrolysis was demonstrated in this study, as well as the usefulness of the ANN technique for thermogravimetric analysis of biomass.
    MeSH terms: Kinetics; Thermodynamics; Thermogravimetry; Neural Networks (Computer)*; Biomass
  11. Hajar,Ar,Rahmah,Nasri,, Mohamad,Shafiq,Mohd,Ibrahim,, Insyirah,Kamarulzaman,, Musliana,Mustaffa,
    Science Letters, 2021;15(2):43-53.
    MyJurnal
    ABSTRACT
    The obturation quality of GuttaFlow Bioseal in curved root canals is not commonly investigated although there has been a current approach toward utilizing this material in extracted molars in recent years. This study assessed the obturated surface area, extrusion of root filling material
    beyond the apical foramen and duration of obturation procedure in curved root canals using monocone obturation technique. Access cavity was prepared in 20 human mandibular molars. Root canals with curvature of more than 10 as determined according to Schneider’s method were
    included. Samples were prepared using Hyflex CM rotary files and divided into two groups (n=10): Group 1 [gutta-percha cone and GuttaFlow Bioseal] and Group 2 [gutta-percha cone and RoekoSeal Automix root canal sealer]. The duration of obturation procedure was recorded and obturation radiographs were taken. Samples were bisected and the mesial roots were sectioned horizontally to obtain 3 root segments; apical, middle and coronal. All resected roots were mounted on brass stubs, sputter-coated with thin platinum coating and observed under scanning electron microscope (SEM) at 70x magnification. The SEM images were transferred to the SketchAndCalc Area Calculator software. No statistically significant differences in the obturated surface area and extrusion of root filling material were observed between Group 1 and 2, irrespective of the status of root canal curvature. Duration for obturation in severe root canal curvatures between Group 1 and 2 were statistically significant. Obturated surface area and extrusion of root filling material were not affected by the root canal curvature, however duration for obturation using GuttaFlow Bioseal in severe root canal curvatures was slightly longer.
  12. Hazwani,Mohd,Yusof,, Sharaniza ,Ab-Rahim,, Wan,Zurinah,Wan,Ngah,, Sheila,Nathan,, A,Rahman,A,Jamal,, Musalmah,Mazlan,
    Science Letters, 2021;15(2):27-36.
    MyJurnal
    ABSTRACT
    Metabolic footprinting involves the determination of metabolites excreted or secreted by the cells.
    This study aimed to identify the differential extracellular metabolites in colorectal cancer (CRC)
    cells for the determination of molecular changes that occur as CRC progresses. CRC cells at
    different stages ie; SW 1116 (stage A), HT 29 and SW 480 (stage B), HCT 15 and DLD-1 (stage
    C), and HCT 116 (stage D) were grown in culture. The media in which the cells were grown are
    subjected to metabolomics profiling using Liquid Chromatography Mass SpectrometryQuadrupole Time of Flight (LC/MS Q-TOF). Statistical and metabolic pathway analysis was
    performed using Metaboanalyst software and identification of metabolites was determined by the
    METLIN database. A total of 27 differential extracellular metabolites were identified in CRC cells
    of different stages compared to stage A cells. Data from the Partial least squares-discriminant
    analysis (PLS-DA) score plot shows a clear separation between CRC cells of different stages with
    a few overlaps between stage B and C. Further analysis using variable importance in projection
    (VIP) revealed 14 differential extracellular metabolites that were most significant in differentiating
    CRC cells of the advanced stages from stage A which are 5-hydroxy-L-tryptophan,
    indoleacetaldehyde, 4,5-dimethylthiazole, 8-oxodiacetoxyscirpenol, bisnorbiotin, 5-amino-6-
    (5'phosphoribosylamino) uracil, glyceryl 5-hydroxydecanoate, sphinganine, 8,8-diethoxy-2,6-
    dimethyl-2-octanol, l-cystine, thiamine acetic acid, phytosphingosine, PE
    (20:4(5Z,8Z,11Z,14Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)), N-(2R-hydroxypentacosano-yl)-2Samino-1,3S,4R-octadecanetriol. The different expressions of metabolites may indicate altered
    metabolic pathways in the more advanced CRC cells compared to stage A. This study highlights
    the importance of conducting both metabolomics profiling of extracellular and intracellular to
    generate a more complete understanding on the molecular changes that occur as CRC progresses
  13. Nurul,Elyni,Mat,Shaari,, Deny,Susanti,, Shafida,Abd,Hamid,
    Science Letters, 2021;15(2):14-22.
    MyJurnal
    ABSTRACT
    Calcium oxalate is one of the most common components in urolithiasis. Its treatment includes the
    use of synthetic drugs, ultrasound and surgery. However, cheaper alternative treatment using
    herbal medicine with less adverse side effect is preferred. Essential oils from Thai basil (Ocimum
    bacilicum L.), Vietnamese coriander (Persicaria odorata) and Chinese parsley (Coriandum
    sativum L.) were extracted and investigated for antiurolithic activity based on calcium oxalate
    crystallisation. Most of the crystals formed in control sample were hexagonal calcium oxalate
    monohydrate with sizes ranging between 3 to 4 m. The size of the crystals was found to be slightly
    reduced in O. bacilicum oil (2-4 m) at high concentration with less aggregation of crystals.
    Samples with P. odorata oil gave smaller crystal size (3 m) mainly in dehydrate form and the oil
    was also found to inhibit the aggregation of the crystals at high concentration. C. sativum oil
    enhanced crystallisation (5-6 m) with increased concentration and showed high aggregation of
    the crystals. This preliminary study shows the therapeutic potential of these medicinal plants to be
    used in traditional anti-urolithic therapy.
  14. Ajmal,Jahid,, Harinder,Rai,Singh,
    Science Letters, 2021;15(2):2-9.
    MyJurnal
    ABSTRACT
    This study reports on the abundance and distribution of mangrove gastropods from Kuala Selangor
    Nature Park, Malaysia (KSNP). The line transect method with quadrats (5m x 5m) was employed
    to sample the gastropods. Gastropods were collected from the leaves, branches, stems and roots of
    trees and the mangrove floor. Nineteen gastropod taxa from seven families were recorded from the
    Bruguiera, mixed (Bruguiera, Avicennia and Rhizophora), Avicennia and Rhizophora zones.
    Avicennia sp. recorded the largest Girth at Breast Height (GBH) (mean±sd=37.3±18.2 cm) while
    Bruguiera sp. recorded the smallest GBH (mean=22.36±8 cm). The overall density of the gastropods at KSNP was 0.85 no/m2.The gastropod density was higher at the Avicennia & Rhizophora zone (150-200m (closer to the mudflats) (0.064±0.084 no/m2) followed by the mixed
    zone (50-150m) (0.046±0.30 no/m2) and lowest at the Bruguiera zone (0-50m) (0.08±0.080 no/m2) (closer to the coastal bund). Gastropod density between sampling zones did not vary significantly (p>0.05) but generally increased from the bund towards the mudflat edge. Further
    studies of the role of gastropods in the coastal food chain, as bio-indicators of habitat change and as potential food source for local populace are needed.
  15. Shafie AA, Chhabra IK, Wong JHY, Mohammed NS
    Eur J Health Econ, 2021 Jul;22(5):735-747.
    PMID: 33860379 DOI: 10.1007/s10198-021-01287-z
    PURPOSE: To develop a mapping algorithm for generating EQ-5D-3L utility scores from the PedsQL Generic Core Scales (PedsQL GCS) in patients with transfusion-dependent thalassemia (TDT).

    METHODS: The algorithm was developed using data from 345 TDT patients. Spearman's rank correlation was used to evaluate the conceptual overlap between the instruments. Model specifications were chosen using a stepwise regression. Both direct and response mapping methods were attempted. Six mapping estimation methods ordinary least squares (OLS), a log-transformed response using OLS, generalized linear model (GLM), two-part model (TPM), Tobit and multinomial logistic regression (MLOGIT) were tested to determine the root mean squared error (RMSE) and mean absolute error (MAE). Other criterion used were accuracy of the predicted utility score, proportions of absolute differences that was less than 0.03 and intraclass correlation coefficient. An in-sample, leave-one-out cross validation was conducted to test the generalizability of each model.

    RESULTS: The best performing model was specified with three out of the four PedsQL GCS scales-the physical, emotional and social functioning score. The best performing estimation method for direct mapping was a GLM with a RMSE of 0.1273 and MAE of 0.1016, while the best estimation method for response mapping was the MLOGIT with a RMSE of 0.1597 and MAE of 0.0826.

    CONCLUSION: The mapping algorithm developed using the GLM would facilitate the calculation of utility scores to inform economic evaluations for TDT patients when EQ-5D data is not available. However, caution should be exercised when using this algorithm in patients who have poor quality of life.

    MeSH terms: Algorithms; Cost-Benefit Analysis; Humans; Quality of Life*; Surveys and Questionnaires; Thalassemia*; Linear Models
  16. Alyan E, Saad NM, Kamel N, Rahman MA
    Appl Ergon, 2021 Oct;96:103497.
    PMID: 34139374 DOI: 10.1016/j.apergo.2021.103497
    This study aims to evaluate the effect of workstation type on the neural and vascular networks of the prefrontal cortex (PFC) underlying the cognitive activity involved during mental stress. Workstation design has been reported to affect the physical and mental health of employees. However, while the functional effects of ergonomic workstations have been documented, there is little research on the influence of workstation design on the executive function of the brain. In this study, 23 healthy volunteers in ergonomic and non-ergonomic workstations completed the Montreal imaging stress task, while their brain activity was recorded using the synchronized measurement of electroencephalography and functional near-infrared spectroscopy. The results revealed desynchronization in alpha rhythms and oxygenated hemoglobin, as well as decreased functional connectivity in the PFC networks at the non-ergonomic workstations. Additionally, a significant increase in salivary alpha-amylase activity was observed in all participants at the non-ergonomic workstations, confirming the presence of induced stress. These findings suggest that workstation design can significantly impact cognitive functioning and human capabilities at work. Therefore, the use of functional neuroimaging in workplace design can provide critical information on the causes of workplace-related stress.
    MeSH terms: Neurovascular Coupling*; Electroencephalography; Humans; Workplace; Prefrontal Cortex; Spectroscopy, Near-Infrared
  17. Hamzah SR, Altrawneh RS, Anuar ST, Khalik WMAWM, Kolandhasamy P, Ibrahim YS
    Mar Pollut Bull, 2021 Sep;170:112617.
    PMID: 34139586 DOI: 10.1016/j.marpolbul.2021.112617
    In this study, the ingestion of microplastics by the deposit-feeding polychaete Namalycastis sp. in the estuarine area of the Setiu Wetlands, Malaysia was confirmed. Samples were collected from six stations, covering the wetland from the south to the north, bimonthly between November 2016 and November 2017. Microplastics were extracted from polychaete samples following digestion in an alkaline solution (10 M NaOH). They were identified by physical characteristics (i.e., shape and color under dissecting microscope and scanning electron microscope), and chemical analysis using a LUMOS Fourier Transform Infrared Microscope (μ-FTIR). A total of 3277 pieces were identified, which were dominated by filaments (99.79%) and with the majority transparent in color (84.71%). Most of the microplastics identified were polypropylene (PP) followed by polyamide (PA) based on their main peak in the of μ-FTIR spectrum. Principal component analysis demonstrated the dominance of microplastics at stations 3 and 4 of the sampling area, probably because of the influx from the open sea and from aquaculture. The findings of this research provide baseline information on microplastics ingested by benthic organisms and their fate in the estuarine food web.
    MeSH terms: Eating; Environmental Monitoring; Malaysia; Plastics; Wetlands
  18. Sonne C, Peng WX, Alstrup AKO, Lam SS
    Science, 2021 Jun 18;372(6548):1271.
    PMID: 34140374 DOI: 10.1126/science.abj3359
    MeSH terms: Animals; Conservation of Natural Resources; Eels*; Population Dynamics; Endangered Species*
  19. Ayat Ali AS, Lim SK, Tang LY, Rashid AA, Chew BH
    Sci Prog, 2021;104(2):368504211026159.
    PMID: 34143698 DOI: 10.1177/00368504211026159
    The complexity of chronic kidney disease (CKD) and its treatments have made self-management behaviors inevitably challenging. However, supplementing education with self-management skills may improve numerous health outcomes in people with nondialysis CKD. This study protocol describes a randomized controlled trial (RCT) aimed to evaluate the effects of a nurse-led self-management support program as an intervention for kidney disease knowledge and CKD self-management behaviors among people with pre-dialysis CKD. In Phase 1, people with CKD stage 3-4 and their family members are involved in co-designing, development and pilot testing of a theory-based self-management intervention. In Phase 2, we perform a cross-cultural adaptation of the Kidney Disease Knowledge Survey, CKD Self-Management and Self-efficacy for Managing Chronic Disease questionnaires. In Phase 3, a parallel RCT will be conducted to evaluate the intervention where 154 participants with CKD stage 3-4 will be randomly assigned to either the intervention (n = 77) or control group (n = 77). The intervention group will receive 6-week self-management program from a nurse-coach in addition to standard usual care, while the control group will receive only standard usual care. Outcome measures include kidney disease knowledge, CKD self-management behavior, self-efficacy, quality of life, blood pressure control and adherence to CKD diet as indicated by 24-h urine urea nitrogen, 24-h urine sodium and net endogenous acid production. Data will be collected at baseline and 12-week post-baseline. The between- and within-group intervention effects will be estimated using the Generalized Estimating Equations. The self-management intervention offers strategies to delay CKD progression and to encourage motivation to better self-manage at home. This study integrates self-management education and psychosocial support with culturally relevant scenarios, and evaluates important self-reported and objective outcomes.Clinical Trials Registration: www.ClinicalTrials.gov, identifier: NCT03974646.
    MeSH terms: Blood Pressure; Female; Humans; Male; Randomized Controlled Trials as Topic; Self Efficacy; Nurse's Role
  20. Webair HH, Ismail TAT, Ismail SB, Khaffaji AJ
    BMJ Open, 2021 06 18;11(6):e044300.
    PMID: 34145008 DOI: 10.1136/bmjopen-2020-044300
    OBJECTIVE: The current study aims to define patient-centred infertility care (PCIC) from the perspective of Arab women with infertility.

    DESIGN: Semistructured in-depth telephone interviews.

    SETTING: Hospitals providing infertility care, Jeddah, Saudi Arabia.

    PARTICIPANTS: Arab women who received infertility treatment during the 6 months preceding the interview at any hospital in Jeddah, Saudi Arabia. Interviews were conducted with Arab women experiencing infertility from January 2017 to December 2018. A purposive sample of 14 women were included in the final analysis with maximum variation.

    RESULTS: Participants highlighted nine important PCIC dimensions. Of these, four were agreed on by all participants: accessibility, minimising cost, information and education, and staff attitudes and communication. The remaining five dimensions were staff competence, physical comfort, privacy, psychological and emotional support, and continuity and coordination of care. The concept of PCIC was related to three major contributors: participants' demographics, patient experience with infertility care and health-seeking behaviour.

    CONCLUSIONS: The current study provided nine PCIC dimensions and items, which can guide efforts to improve the quality of infertility care in Arab countries in two ways: first, by raising infertility care providers' awareness of their patients' needs, and second, by developing a validated tool based on the dimensions for measuring PCIC from Arab patients' perspective. Clear differences between the Arab and the European PCIC model were found. Our findings concluded that women continued to exhibit basic unmet needs.

    MeSH terms: Female; Humans; Saudi Arabia; Patient-Centered Care; Arabs*; Qualitative Research
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