Browse publications by year: 2021

  1. Zhang H, Liu S, Wu K, Cui J, Zhu A, Zhang Y, et al.
    Mar Pollut Bull, 2021 Jul;168:112433.
    PMID: 33962084 DOI: 10.1016/j.marpolbul.2021.112433
    The heavy metal contents (Cr, Cu, Zn, Cd, Pb, Hg, and As) of 88 surface sediment samples from the western Sunda Shelf were analyzed to determine their spatial distribution patterns and contamination status. The results demonstrated that high enrichment regions of heavy metals were focused in the Kelantan, Pahang, and Ambat river estuaries, and deep water regions of the study area. These high enrichment regions were mainly controlled by riverine inputs and their hydrodynamic conditions. The enrichment factor (EF), geoaccumulation index (Igeo), and potential ecological risk index (PERI) were used to assess heavy metal accumulation. The results indicated that the study area was not significantly contaminated overall at the time of the study; however, Cd, As, and Hg were at levels corresponding to moderate contamination at many stations located in the Pahang River estuary, Kelantan River estuary, and north-eastern region of the study area, primarily because of anthropogenic activities.
    MeSH terms: China; Environmental Monitoring; Risk Assessment; Geologic Sediments; Rivers; Estuaries
  2. Cerea S, Todd J, Ghisi M, Mancin P, Swami V
    Body Image, 2021 Sep;38:210-218.
    PMID: 33962221 DOI: 10.1016/j.bodyim.2021.04.007
    The Functionality Appreciation Scale (FAS; Alleva et al., 2017) is a widely used instrument for the measurement of individual's appreciation of their body for what it can do and is capable of doing (i.e., functionality appreciation). Here, we examined the psychometric properties of a novel Italian translation of the FAS. A sample of 950 Italian adults completed the FAS, as well as previously validated measures of body image (body appreciation, body esteem, body surveillance), disordered eating symptoms, and psychological well-being (self-esteem, general distress). Exploratory and confirmatory factor analyses supported a 1-dimensional model of FAS scores, with all 7 items retained. Scores achieved scalar invariance across gender, and the gender difference in latent FAS scores was not significant. FAS scores were found to have adequate internal consistency, test-retest reliability up to three weeks, and convergent and criterion-related validity through significant correlations with all additional constructs. However, evidence of incremental validity was weak, and likely reflected high nomological and conceptual similarity between functionality and body appreciation in this national context. Overall, these results provide strong evidence that scores on the Italian FAS are psychometrically valid, which may aid future development of interventions to promote more positive body image in Italian-speaking samples.
    MeSH terms: Adult; Factor Analysis, Statistical; Humans; Italy; Psychometrics; Surveys and Questionnaires*; Translations; Reproducibility of Results
  3. Lee JL, Mohamed Shah N, Makmor-Bakry M, Islahudin F, Alias H, Mohd Saffian S
    Int Immunopharmacol, 2021 Aug;97:107721.
    PMID: 33962225 DOI: 10.1016/j.intimp.2021.107721
    BACKGROUND: Population pharmacokinetics (popPK) using the nonlinear mixed-effect (NLME) modeling approach is an essential tool for guiding dose individualization. Several popPK analyses using the NLME have been conducted to characterize the pharmacokinetics of immunoglobulin G (IgG).

    OBJECTIVE: To summarize the current information on popPK of polyclonal IgG therapy.

    METHOD: A systematic search was conducted in the PubMed and Web of Science databases from inception to December 2020. Additional relevant studies were also included by reviewing the reference list of the reviewed articles. All popPK studies that employed the NLME modeling approach were included and data were synthesized descriptively.

    RESULTS: This review included seven studies. Most of the popPK models were developed in patients with primary immunodeficiency (PID). IgG pharmacokinetics was described as a two-compartment model in five studies, while it was described as a one-compartment model in two other studies. Among all tested covariates, weight was consistently identified as a significant predictor for clearance (CL) of IgG. Whereas, weight and disease type were found to be significant predictors for the volume of distribution in central compartment (Vc). In a typical 70 kg adult, the median estimated values of Vc and CL were 4.04 L and 0.144 L/day, respectively. The between subject variability of Vc was considered large. Only two studies evaluated their models using external data.

    CONCLUSIONS: Seven popPK studies of IgG were found and discussed, with only weight being a significant covariate across all studies. Future studies linking pharmacokinetics with pharmacodynamics in PID and other patient populations are required.

  4. Chen WH, Lo HJ, Yu KL, Ong HC, Sheen HK
    Environ Pollut, 2021 Sep 15;285:117196.
    PMID: 33962308 DOI: 10.1016/j.envpol.2021.117196
    This research aims to study the wet torrefaction (WT) and saccharification of sorghum distillery residue (SDR) towards hydrochar and bioethanol production. The experiments are designed by Box-Behnken design from response surface methodology where the operating conditions include sulfuric acid concentration (0, 0.01, and 0.02 M), amyloglucosidase concentration (36, 51, and 66 IU), and saccharification time (120, 180, and 240 min). Compared to conventional dry torrefaction, the hydrochar yield is between 13.24 and 14.73%, which is much lower than dry torrefaction biochar (yield >50%). The calorific value of the raw SDR is 17.15 MJ/kg, which is significantly enhanced to 22.36-23.37 MJ/kg after WT. When the sulfuric acid concentration increases from 0 to 0.02 M, the glucose concentration in the product increases from 5.59 g/L to 13.05 g/L. The prediction of analysis of variance suggests that the best combination to maximum glucose production is 0.02 M H2SO4, 66 IU enzyme concentration, and 120 min saccharification time, and the glucose concentration is 30.85 g/L. The maximum bioethanol concentration of 19.21 g/L is obtained, which is higher than those from wheat straw (18.1 g/L) and sweet sorghum residue (16.2 g/L). A large amount of SDR is generated in the kaoliang liquor production process, which may cause environmental problems if it is not appropriately treated. This study fulfills SDR valorization for hydrochar and bioenergy to lower environmental pollution and even achieve a circular economy.
    MeSH terms: Edible Grain; Environmental Pollution; Triticum; Sorghum*
  5. Chu SY, Lee J, Barlow SM, Ben-David B, Lim KX, Foong JH
    Int J Speech Lang Pathol, 2021 08;23(4):419-429.
    PMID: 33059474 DOI: 10.1080/17549507.2020.1808701
    PURPOSE: This study examined the effects of non-word versus real word, age, and gender on oral-DDK rates among healthy Malaysian-Mandarin speakers. Comparison between non-word of Malaysian-Mandarin and Hebrew speakers was examined.

    METHOD: One-hundred and seventeen speakers (18-83 years old, 46% men) were audio-recorded while performing non-word (repetition of "pataka") and real-word oral-DDK tasks ("butter cake" and " ([pha4tha1khan4])"). The number of syllables produced in 8 seconds was counted from the audio recording to derive the oral-DDK rates. A MANOVA was conducted to compare the rates between age groups (young = 18-40 years, n = 56; middle = 41-60 years, n = 39; older = 61-83 years, n = 22) and gender. In a second analysis, "pataka" results were compared between this study and previous findings with Hebrew speakers.

    RESULT: No gender effects were found. However, rates significantly decreased with age (p  non-words (5.29 ± 1.23) > Mandarin words (4.91 ± 1.13). Malaysian-Mandarin speakers performed slower than Hebrew speakers on "pataka" task.

    CONCLUSION: Aging has a large impact on oromotor functions, indicating that speech-language pathologists should consider using age-adjusted norms.

    MeSH terms: Adolescent; Adult; Aged; Aged, 80 and over; Aging; Female; Humans; Language*; Linguistics*; Male; Middle Aged; Young Adult
  6. Wong KY, Tan KY, Tan NH, Tan CH
    Toxins (Basel), 2021 01 14;13(1).
    PMID: 33466660 DOI: 10.3390/toxins13010060
    The Senegalese cobra, Naja senegalensis, is a non-spitting cobra species newly erected from the Naja haje complex. Naja senegalensis causes neurotoxic envenomation in Western Africa but its venom properties remain underexplored. Applying a protein decomplexation proteomic approach, this study unveiled the unique complexity of the venom composition. Three-finger toxins constituted the major component, accounting for 75.91% of total venom proteins. Of these, cardiotoxin/cytotoxin (~53%) and alpha-neurotoxins (~23%) predominated in the venom proteome. Phospholipase A2, however, was not present in the venom, suggesting a unique snake venom phenotype found in this species. The venom, despite the absence of PLA2, is highly lethal with an intravenous LD50 of 0.39 µg/g in mice, consistent with the high abundance of alpha-neurotoxins (predominating long neurotoxins) in the venom. The hetero-specific VINS African Polyvalent Antivenom (VAPAV) was immunoreactive to the venom, implying conserved protein antigenicity in the venoms of N. senegalensis and N. haje. Furthermore, VAPAV was able to cross-neutralize the lethal effect of N. senegalensis venom but the potency was limited (0.59 mg venom completely neutralized per mL antivenom, or ~82 LD50 per ml of antivenom). The efficacy of antivenom should be further improved to optimize the treatment of cobra bite envenomation in Africa.
    MeSH terms: Animals; Antivenins/therapeutic use*; Cobra Neurotoxin Proteins/analysis; Cobra Neurotoxin Proteins/toxicity; Elapid Venoms/analysis*; Elapid Venoms/toxicity*; Horses; Humans; Lethal Dose 50; Mice, Inbred ICR; Snake Bites/therapy; Elapidae; Neurotoxicity Syndromes/therapy; Proteome/analysis; Mice; Phospholipases A2/analysis*; Antibodies, Neutralizing/immunology
  7. Zhang X, Sun J, Chen F, Qi H, Chen L, Sung YY, et al.
    Microb Genom, 2021 05;7(5).
    PMID: 33952389 DOI: 10.1099/mgen.0.000549
    The virulence of Vibrio parahaemolyticus is variable depending on its virulence determinants. A V. parahaemolyticus strain, in which the virulence is governed by the pirA and pirB genes, can cause acute hepatopancreatic necrosis disease (AHPND) in shrimps. Some V. parahaemolyticus that are non-AHPND strains also cause shrimp diseases and result in huge economic losses, while their pathogenicity and pathogenesis remain unclear. In this study, a non-AHPND V. parahaemolyticus, TJA114, was isolated from diseased Penaeus vannamei associated with a high mortality. To understand its virulence and adaptation to the external environment, whole-genome sequencing of this isolate was conducted, and its phenotypic profiles including pathogenicity, growth characteristics and nutritional requirements were investigated. Shrimps following artificial infection with this isolate presented similar clinical symptoms to the naturally diseased ones and generated obvious pathological lesions. The growth characteristics indicated that the isolate TJA114 could grow well under different salinity (10-55 p.p.t.), temperature (23-37 °C) and pH (6-10) conditions. Phenotype MicroArray results showed that this isolate could utilize a variety of carbon sources, amino acids and a range of substrates to help itself adapt to the high hyperosmotic and alkaline environments. Antimicrobial-susceptibility test showed that it was a multidrug-resistant bacterium. The whole-genomic analysis showed that this V. parahaemolyticus possessed many important functional genes associated with multidrug resistance, stress response, adhesions, haemolysis, putative secreted proteases, dedicated protein secretion systems and a variety of nutritional metabolic mechanisms. These annotated functional genes were confirmed by the phenotypic profiles. The results in this study indicated that this V. parahaemolyticus isolate possesses a high pathogenicity and strong environmental adaptability.
    MeSH terms: Adaptation, Physiological; Animals; Animal Diseases/microbiology*; Bacterial Proteins/genetics; Microbial Sensitivity Tests; Phenotype*; Vibrio parahaemolyticus/genetics*; Virulence; Genomics*; DNA Gyrase/genetics; Penaeidae/genetics; Penaeidae/microbiology*; Virulence Factors/genetics
  8. Lim MT, Ong SM, Tong SF, Groenewegen P, Sivasampu S
    BMJ Open, 2021 05 05;11(5):e047126.
    PMID: 33952553 DOI: 10.1136/bmjopen-2020-047126
    OBJECTIVES: Most countries including Malaysia have set goals to incorporate a strong primary care into the healthcare system. The aim of this study was to evaluate the strength of service delivery process dimensions in Malaysia and compare it with England, the Netherlands, Spain, North Macedonia, Romania and Turkey which participated in the Quality and Costs of Primary Care (QUALICOPC) study.

    METHODS: This cross-sectional study utilised the QUALICOPC study data on primary care performance, which was conducted in 2011-2013 (QUALICOPC in Europe Australia, New Zealand and Canada) and 2015-2016 (Malaysia). A standardised questionnaire was completed by primary care practitioners from participating countries. Multilevel regression analysis and composite scores were constructed to compare the performance of primary care on four process dimensions: accessibility, comprehensiveness, continuity of care and coordination.

    RESULTS: The high-income countries with strong primary care performed better in comprehensiveness, continuity and coordination but poorer in accessibility to services compared with upper-middle-income countries. Among the upper-middle-income countries, Malaysia scored the best in comprehensiveness and coordination. None of the studied countries were having consistent performance over all indicators either in their respective best or worst primary care services delivery dimensions.

    CONCLUSIONS: There is a wide variation in primary care services delivery across and within the studied countries. The findings indicate room for quality improvement activities to strengthen primary healthcare services. This includes addressing current healthcare challenges in response to the population health needs which are essential for more integrated and efficient primary care services delivery.

    MeSH terms: Australia; Canada; Cross-Sectional Studies; Delivery of Health Care*; England; Europe; Humans; Malaysia; Netherlands; New Zealand; Primary Health Care*; Romania; Spain; Turkey
  9. Yanagisawa D, Hamezah HS, Pahrudin Arrozi A, Tooyama I
    Sci Rep, 2021 May 05;11(1):9623.
    PMID: 33953293 DOI: 10.1038/s41598-021-89142-2
    Tau, a family of microtubule-associated proteins, forms abnormal intracellular inclusions, so-called tau pathology, in a range of neurodegenerative diseases collectively known as tauopathies. The rTg4510 mouse model is a well-characterized bitransgenic F1 hybrid mouse model of tauopathy, which was obtained by crossing a Camk2α-tTA mouse line (on a C57BL/6 J background) with a tetO-MAPT*P301L mouse line (on a FVB/NJ background). The aim of this study was to investigate the effects of the genetic background and sex on the accumulation of tau pathology in reciprocal F1 hybrids of rTg4510 mice, i.e., rTg4510 on the (C57BL/6 J × FVB/NJ)F1 background (rTg4510_CxF) and on the (FVB/NJ × C57BL/6 J)F1 background (rTg4510_FxC). As compared with rTg4510_CxF mice, the rTg4510_FxC mice showed marked levels of tau pathology in the forebrain. Biochemical analyses indicated that the accumulation of abnormal tau species was accelerated in rTg4510_FxC mice. There were strong effects of the genetic background on the differential accumulation of tau pathology in rTg4510 mice, while sex had no apparent effect. Interestingly, midline-1 (Mid1) was identified as a candidate gene associated with this difference and exhibited significant up/downregulation according to the genetic background. Mid1 silencing with siRNA induced pathological phosphorylation of tau in HEK293T cells that stably expressed human tau with the P301L mutation, suggesting the role of Mid1 in pathological alterations of tau. Elucidation of the underlying mechanisms will provide novel insights into the accumulation of tau pathology and is expected to be especially informative to researchers for the continued development of therapeutic interventions for tauopathies.
    MeSH terms: Animals; Brain/metabolism*; Brain/pathology; Inclusion Bodies/metabolism*; Inclusion Bodies/pathology; Disease Models, Animal; Mice, Transgenic; Neurons/metabolism*; Neurons/pathology; Phosphorylation; tau Proteins/genetics; tau Proteins/metabolism*; Tauopathies/genetics; Tauopathies/metabolism*; Tauopathies/pathology; Mice
  10. Lee TH, Wani WA, Lee CH, Cheng KK, Shreaz S, Wong S, et al.
    Front Pharmacol, 2021;12:626233.
    PMID: 33953670 DOI: 10.3389/fphar.2021.626233
    Edible Bird's Nest (EBN) is the most prized health delicacy among the Chinese population in the world. Although some scientific characterization and its bioactivities have been studied and researched, no lights have been shed on its actual composition or mechanism. The aim of this review paper is to address the advances of EBN as a therapeutic animal bioproduct, challenges and future perspectives of research involving EBN. The methodology of this review primarily involved a thorough search from the literature undertaken on Web of Science (WoS) using the keyword "edible bird nest". Other information were obtained from the field/market in Malaysia, one of the largest EBN-producing countries. This article collects and describes the publications related to EBN and its therapeutic with diverse functional values. EBN extracts display anti-aging effects, inhibition of influenza virus infection, alternative traditional medicine in athletes and cancer patients, corneal wound healing effects, stimulation of proliferation of human adipose-derived stem cells, potentiate of mitogenic response, epidermal growth factor-like activities, enhancement of bone strength and dermal thickness, eye care, neuroprotective and antioxidant effects. In-depth literature study based on scientific findings were carried out on EBN and its properties. More importantly, the future direction of EBN in research and development as health-promoting ingredients in food and the potential treatment of certain diseases have been outlined.
    MeSH terms: Aging; Animals; Antioxidants; Birds; Epidermal Growth Factor; Humans; Influenza, Human; Malaysia; Medicine, Traditional; Mitogens; Neoplasms; Orthomyxoviridae Infections; Plant Extracts; Research; Saliva; Stem Cells; Wound Healing; Asian Continental Ancestry Group; Cell Proliferation; Athletes; Corneal Injuries
  11. Yusof ENM, Kwong HC, Karunakaran T, Ravoof TBSA, Tiekink ERT
    Acta Crystallogr E Crystallogr Commun, 2021 Mar 01;77(Pt 3):286-293.
    PMID: 33953953 DOI: 10.1107/S2056989021001870
    The title diorganotin Schiff base derivative, [Sn(C4H9)2(C15H13N3O2S)], features a penta-coordinated tin centre defined by the N,O,S-donor atoms of the di-anionic Schiff base ligand and two methyl-ene-C atoms of the n-butyl substituents. The resultant C2NOS donor set defines a geometry inter-mediate between trigonal-bipyramidal and square-pyramidal. In the crystal, amine-N-H⋯O(meth-oxy) hydrogen bonding is found in a helical, supra-molecular chain propagating along the b-axis direction. The chains are assembled into a layer parallel to (01) with methyl-ene-C-H⋯π(phen-yl) inter-actions prominent; layers stack without directional inter-actions between them. The analysis of the calculated Hirshfeld surface showed the presence of weak methyl-ene-C-H⋯π(phen-yl) inter-actions and short H⋯H contacts in the inter-layer region. Consistent with the nature of the identified contacts, the stabilization of the crystal is dominated by the dispersion energy term.
  12. Kamaruddin NC, Razali MAI, Busayo IE, Hamzah NH, Idris LH, Md Isa NM
    J Parasitol Res, 2021;2021:6691483.
    PMID: 33953962 DOI: 10.1155/2021/6691483
    Ruminant fascioliasis is a neglected yet important tropical zoonotic disease that affects both the livestock and humans. The disease has a worldwide distribution, and Malaysia is one of the countries that face problems related to this parasite. These retrospective studies were conducted in Makmal Diagnosa Veterinar Kota Kinabalu (MDVKK) and Sabah Meat Technology Centre (SMTC), Kinarut over a period of eleven years (2008-2018). For MDVKK, the overall occurrence of fascioliasis was 24.9%. Out of 769 cattle's and buffaloes' faecal samples submitted, Fasciola spp ova were detected in 189 of the samples. A total of 2297 cattle, buffaloes, and goats were slaughtered at SMTC over that period, and 21 livers were condemned due to fascioliasis, giving the total occurrence of 0.91%. This investigation provides information on the occurrence of ruminant fascioliasis in Sabah, East Malaysia. The results from this study highlight the alarming incidence of fascioliasis and the urgent need for action to control this neglected tropical disease in East Malaysia.
  13. Ahmad MH, Salleh R, Siew Man C, Pardi M, Che Abdul Rahim N, Shahril N, et al.
    J Nutr Metab, 2021;2021:6639935.
    PMID: 33953978 DOI: 10.1155/2021/6639935
    Background: Malaysia is predicted to become an ageing population by 2035. Malnutrition among the elderly is one of growing concern. This study aims to identify the prevalence of malnutrition and its associated factors among the elderly in Malaysia.

    Methods: Data from the National Health and Morbidity Survey (NHMS) 2018 was analysed. This survey applied a multistage stratified cluster sampling design to ensure national representativeness. Malnutrition was identified using a validated Mini Nutrition Assessment-Short Form (MNA-SF). Variables on sociodemographic, health status, and dietary practices were also obtained. The complex sampling analysis was used to determine the prevalence and associated factors of at-risk or malnutrition among the elderly.

    Result: A total of 3,977 elderly completed the MNA-SF. The prevalence of malnutrition and at-risk of malnutrition was 7.3% and 23.5%, respectively. Complex sample multiple logistic regression found that the elderly who lived in a rural area, with no formal or primary level of education, had depression, Instrumental Activity of Daily Living (IADL) dependency, and low quality of life (QoL), were underweight, and had food insecurity and inadequate plain water intake were at a significant risk of malnutrition (malnutrition and at-risk), while Chinese, Bumiputra Sarawak, and BMI more than 25 kgm-2 were found to be protective.

    Conclusions: Currently, three out of ten elderly in Malaysia were at-risk or malnutrition. The elderly in a rural area, low education level, depression, IADL dependency, low QoL, underweight, food insecurity, and inadequate plain water intake were at risk of malnutrition in Malaysia. The multiagency approach is needed to tackle the issue of malnutrition among the elderly by considering all predictors identified from this study.

    MeSH terms: Aged; Aging; Depression; Drinking; Educational Status; Health Status; Health Surveys; Humans; Malaysia; Quality of Life; Surveys and Questionnaires; Thinness; Nutrition Assessment; Body Mass Index; Prevalence; Logistic Models; Malnutrition; Asian Continental Ancestry Group
  14. Heboyan A, Avetisyan A, Skallevold HE, Rokaya D, Marla V, Vardanyan A
    Case Rep Dent, 2021;2021:6648729.
    PMID: 33953989 DOI: 10.1155/2021/6648729
    Recurrent aphthous stomatitis is an ulcerative disease of the oral cavity and can occur in isolation or as a manifestation of many systemic diseases. It is a quite common entity and may hence often be overlooked as an isolated lesion. Gilbert's syndrome is a genetic disorder where a deficiency of an enzyme associated with the conjugation of bilirubin results in unconjugated hyperbilirubinemia. The disease is generally asymptomatic and is aggravated by certain trigger factors. No associated oral manifestations are known. In this case report, we discuss the concomitant presence of recurrent aphthous stomatitis in a patient of Gilbert's syndrome. The presence of such recurrent stomatitis may represent as an oral manifestation of Gilbert's syndrome. Early identification of these entities may improve the overall quality of life of the patient.
    MeSH terms: Bilirubin; Biological Phenomena; Gilbert Disease; Humans; Hyperbilirubinemia; Peptic Ulcer; Quality of Life; Stomatitis, Aphthous
  15. Fazhan H, Waiho K, Fujaya Y, Rukminasari N, Ma H, Ikhwanuddin M
    PeerJ, 2021;9:e10936.
    PMID: 33954025 DOI: 10.7717/peerj.10936
    Sexual dimorphism is a common phenomenon in the animal kingdom. To test the consistency of sexual dimorphism patterns among sympatric species of the same genus, ten morphometric characteristics of mud crabs Scylla olivacea, S. tranquebarica and S. paramamosain were measured and compared using Discriminant Function Analysis (DFA). The descriptive analysis revealed that in all three species, body size dimensions and cheliped dimensions were significantly larger in males whereas the abdomen width was female-biased. Also, we described a morphological variation (carapace width, CW ≤ CW at spine 8, 8CW) that is unique to S. olivacea. Discriminant function analysis revealed that all nine morphometric characteristics were sexually dimorphic in S. olivacea, S. tranquebarica (except right cheliped's merus length, ML) and S. paramamosain (except 8CW). The obtained discriminant functions based on the morphometric ratios (with CW as divisor) correctly classified 100% of adults of known sex of all three species. Further, based on the selected body traits, DFA was able to almost completely distinguish males (94%), but not females (74%), among the three Scylla species. This study highlights that congeneric species of portunids (e.g., Scylla spp.) show similar sexually dimorphic characteristics (body size and secondary sexual characteristics).
    MeSH terms: Abdomen; Animals; Body Weights and Measures; Brachyura; Female; Male; Phenotype; Sex Characteristics; Body Size; Animal Shells; Torso; Sympatry
  16. Charles APT, Shukrimi BA, Zamzuri BZ, Ardilla HBAR
    J Orthop Case Rep, 2021 5 7;10(3):108-113.
    PMID: 33954149 DOI: 10.13107/jocr.2020.v10.i03.1772
    Introduction: The prevalence of knee osteoarthritis is on the raise. This raise has been a huge financial burden to developed countries in treating the disease. Transcutaneous electrical nerve stimulation (TENS) is a cost-effective, easily available, and self-applicable mode of non-pharmacological pain relieve technique. Despite these advantages, the use, settings, and effectiveness of portable TENS are still poorly understood. The aim of this study is to determine the effectiveness of portable TENS at different frequencies in treating knee osteoarthritis.

    Materials and Methods: This is a single-center quasi-experimental study involving 100 patients seen in the outpatient department with knee osteoarthritis. They were randomly (computer generated) allocated into two arms (high frequency [H-F] or low frequency [L-F]). H-F is set at 100 Hz and L-F is set at 4 Hz. A baseline assessment is taken with the visual analog score (VAS), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Oxford Knee Score, and Lequesne index. They were instructed to self-administer the TENS therapy as per protocol and followed up at the 4th and 12th week to be reevaluated on the above scores.

    Results: The final results show that both H-F and L-F groups showed improvement in all parameters of the VAS, WOMAC index, Oxford Knee Score, and Lequesne index (73%). Only the pain component of Lequesne index, activities of daily living component of Lequesne index, total Lequesne index, and pain component of WOMAC index shows a statistically significant difference, favoring the H-F group. The H-F group yields a faster result; however, with time the overall effect remains the same in both groups.

    Conclusion: Both H-F and L-F groups show improvement in all the component of Lequesne index, Oxford Knee Score, WOMAC index, and VAS with no statistical difference between the two groups. Although H-F yields a faster result, not everyone is able to tolerate the intensity. Therefore, the selection of H-F or L-F should be done on case basis depending on the severity of symptoms, patient's expectation, and patient's ability to withstand the treatment therapy. Based on this 12th week follow-up, both groups will continue to improve with time. A longer study should be conducted to see it this improvement will eventually plateau off or continue to improve until the patient is symptom free.

    MeSH terms: Activities of Daily Living; Biometry; Computers; Cost-Benefit Analysis; Transcutaneous Electric Nerve Stimulation; Follow-Up Studies; Humans; Motivation; Ontario; Outpatients; Pain; Universities; Osteoarthritis, Hip; Prevalence; Developed Countries; Osteoarthritis, Knee
  17. Ranjbarzadeh R, Jafarzadeh Ghoushchi S, Bendechache M, Amirabadi A, Ab Rahman MN, Baseri Saadi S, et al.
    Biomed Res Int, 2021;2021:5544742.
    PMID: 33954175 DOI: 10.1155/2021/5544742
    The COVID-19 pandemic is a global, national, and local public health concern which has caused a significant outbreak in all countries and regions for both males and females around the world. Automated detection of lung infections and their boundaries from medical images offers a great potential to augment the patient treatment healthcare strategies for tackling COVID-19 and its impacts. Detecting this disease from lung CT scan images is perhaps one of the fastest ways to diagnose patients. However, finding the presence of infected tissues and segment them from CT slices faces numerous challenges, including similar adjacent tissues, vague boundary, and erratic infections. To eliminate these obstacles, we propose a two-route convolutional neural network (CNN) by extracting global and local features for detecting and classifying COVID-19 infection from CT images. Each pixel from the image is classified into the normal and infected tissues. For improving the classification accuracy, we used two different strategies including fuzzy c-means clustering and local directional pattern (LDN) encoding methods to represent the input image differently. This allows us to find more complex pattern from the image. To overcome the overfitting problems due to small samples, an augmentation approach is utilized. The results demonstrated that the proposed framework achieved precision 96%, recall 97%, F score, average surface distance (ASD) of 2.8 ± 0.3 mm, and volume overlap error (VOE) of 5.6 ± 1.2%.
    MeSH terms: Algorithms*; Female; Humans; Lung/virology; Male; Pneumonia, Viral/virology; Radiographic Image Interpretation, Computer-Assisted; Tomography, X-Ray Computed; Cluster Analysis; Neural Networks (Computer); Pandemics*
  18. Bari BS, Islam MN, Rashid M, Hasan MJ, Razman MAM, Musa RM, et al.
    PeerJ Comput Sci, 2021;7:e432.
    PMID: 33954231 DOI: 10.7717/peerj-cs.432
    The rice leaves related diseases often pose threats to the sustainable production of rice affecting many farmers around the world. Early diagnosis and appropriate remedy of the rice leaf infection is crucial in facilitating healthy growth of the rice plants to ensure adequate supply and food security to the rapidly increasing population. Therefore, machine-driven disease diagnosis systems could mitigate the limitations of the conventional methods for leaf disease diagnosis techniques that is often time-consuming, inaccurate, and expensive. Nowadays, computer-assisted rice leaf disease diagnosis systems are becoming very popular. However, several limitations ranging from strong image backgrounds, vague symptoms' edge, dissimilarity in the image capturing weather, lack of real field rice leaf image data, variation in symptoms from the same infection, multiple infections producing similar symptoms, and lack of efficient real-time system mar the efficacy of the system and its usage. To mitigate the aforesaid problems, a faster region-based convolutional neural network (Faster R-CNN) was employed for the real-time detection of rice leaf diseases in the present research. The Faster R-CNN algorithm introduces advanced RPN architecture that addresses the object location very precisely to generate candidate regions. The robustness of the Faster R-CNN model is enhanced by training the model with publicly available online and own real-field rice leaf datasets. The proposed deep-learning-based approach was observed to be effective in the automatic diagnosis of three discriminative rice leaf diseases including rice blast, brown spot, and hispa with an accuracy of 98.09%, 98.85%, and 99.17% respectively. Moreover, the model was able to identify a healthy rice leaf with an accuracy of 99.25%. The results obtained herein demonstrated that the Faster R-CNN model offers a high-performing rice leaf infection identification system that could diagnose the most common rice diseases more precisely in real-time.
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