Browse publications by year: 2023

  1. Alkhadher SAA, Suratman S, Mohd Sallan MIB
    J Environ Manage, 2023 Nov 01;345:118464.
    PMID: 37454570 DOI: 10.1016/j.jenvman.2023.118464
    The spatial and temporal distributions of trace metals in dissolved forms mainly result from anthropogenic and lithogenic contributions. Surface water samples (∼0.5 m) were collected monthly at respective stations from Setiu Wetland. In this study, the behaviour of trace metals in the dissolved phases along the water column from sampling sites in the Setiu Wetland, Malaysia was investigated. In addition, dissolved organic carbon (DOC) and physical parameters such as salinity, temperature, pH and dissolved oxygen (DO) of the surface water were measured in order to evaluate the relationship between trace metals fractionation with different water quality parameters. Size fractionation study of dissolved trace metals using ultrafiltration technique were also carried out and analysed using inductively coupled plasma mass spectrometry (ICP-MS). Correlation of trace metals with other measured parameters was made to furthermore understand the dynamics of trace metals and its fractionated components in this area. The concentration of dissolved trace metals was in the range of 0.001-0.16 μg/L for Cd, 0.12-2.81 μg/L for Cu, 0.01-1.84 μg/L for Pb, 3-17 μg/L for Fe and 1-34 μg/L for Zn, suggesting the input of anthropogenic sources for trace metals such as municipal, industrial, agricultural and domestic discharge. The periodic monitoring and evaluation of trace metals in wetlands and protected tropical areas is highly recommended.
    MeSH terms: Environmental Monitoring/methods; Seasons; Wetlands
  2. Fornace KM, Zorello Laporta G, Vythilingham I, Chua TH, Ahmed K, Jeyaprakasam NK, et al.
    Lancet Infect Dis, 2023 Dec;23(12):e520-e532.
    PMID: 37454671 DOI: 10.1016/S1473-3099(23)00298-0
    Simian malaria from wild non-human primate populations is increasingly recognised as a public health threat and is now the main cause of human malaria in Malaysia and some regions of Brazil. In 2022, Malaysia became the first country not to achieve malaria elimination due to zoonotic simian malaria. We review the global distribution and drivers of simian malaria and identify priorities for diagnosis, treatment, surveillance, and control. Environmental change is driving closer interactions between humans and wildlife, with malaria parasites from non-human primates spilling over into human populations and human malaria parasites spilling back into wild non-human primate populations. These complex transmission cycles require new molecular and epidemiological approaches to track parasite spread. Current methods of malaria control are ineffective, with wildlife reservoirs and primarily outdoor-biting mosquito vectors urgently requiring the development of novel control strategies. Without these, simian malaria has the potential to undermine malaria elimination globally.
    MeSH terms: Animals; Animals, Wild; Brazil; Humans; Primates; Mosquito Vectors
  3. Kurniawan R, Budi Alamsyah AR, Fudholi A, Purwanto A, Sumargo B, Gio PU, et al.
    Environ Pollut, 2023 Oct 01;334:122212.
    PMID: 37454714 DOI: 10.1016/j.envpol.2023.122212
    The high concentration of nitrogen dioxide (NO2) is to blame for West Java's poor Air Quality Index (AQI). So, this study aims to determine the influence of industrial activity as reflected by the value of its imports and exports, wind speed, and ozone (O3) on the high concentration of tropospheric NO2. The method used is the econometric Vector Error Correction Model (VECM) approach to capture the existence of a short-term and long-term relationship between tropospheric NO2 and its predictor variables. The data used in this study is in the form of monthly time series data for the 2018-2022 period sourced from satellite images (Sentinel-5P and ECMWF Climate Reanalysis) and publications of the Central Bureau of Statistics (BPS-Statistics Indonesia). The results explained that, in the short-term, tropospheric NO2 and O3 influence each other as they would in a photochemical reaction. In the long-term, exports from the industrial sector and wind speed have a significant effect on the concentration of tropospheric NO2. The short-term effect occurs directly in the first month after the shock, while the long-term effect occurs in the second month after the shock. Wind gusts originating from industrial areas cause air conditions to be even more alarming because tropospheric NO2 pollutants spread throughout the region in West Java. Based on the coefficient correlation result, the high number of pneumonia cases is one of the impacts caused by air pollution.
    MeSH terms: Environmental Monitoring/methods; Nitrogen Dioxide/analysis; Remote Sensing Technology
  4. Ravindiran G, Hayder G, Kanagarathinam K, Alagumalai A, Sonne C
    Chemosphere, 2023 Oct;338:139518.
    PMID: 37454985 DOI: 10.1016/j.chemosphere.2023.139518
    Clean air is critical component for health and survival of human and wildlife, as atmospheric pollution is associated with a number of significant diseases including cancer. However, due to rapid industrialization and population growth, activities such as transportation, household, agricultural, and industrial processes contribute to air pollution. As a result, air pollution has become a significant problem in many cities, especially in emerging countries like India. To maintain ambient air quality, regular monitoring and forecasting of air pollution is necessary. For that purpose, machine learning has emerged as a promising technique for predicting the Air Quality Index (AQI) compared to conventional methods. Here we apply the AQI to the city of Visakhapatnam, Andhra Pradesh, India, focusing on 12 contaminants and 10 meteorological parameters from July 2017 to September 2022. For this purpose, we employed several machine learning models, including LightGBM, Random Forest, Catboost, Adaboost, and XGBoost. The results show that the Catboost model outperformed other models with an R2 correlation coefficient of 0.9998, a mean absolute error (MAE) of 0.60, a mean square error (MSE) of 0.58, and a root mean square error (RMSE) of 0.76. The Adaboost model had the least effective prediction with an R2 correlation coefficient of 0.9753. In summary, machine learning is a promising technique for predicting AQI with Catboost being the best-performing model for AQI prediction. Moreover, by leveraging historical data and machine learning algorithms enables accurate predictions of future urban air quality levels on a global scale.
    MeSH terms: Machine Learning; Cities; Environmental Monitoring/methods; Humans; Particulate Matter/analysis
  5. Arul P, Nandhini C, Huang ST, Gowthaman NSK
    Anal Chim Acta, 2023 Sep 15;1274:341582.
    PMID: 37455066 DOI: 10.1016/j.aca.2023.341582
    Tannic acid (TA) is a water-soluble polyphenol and used in beverages, medical fields as clarifying and additive agents. In daily life, TA is unavoidable, and excessive consumption of tannin containing foods can harm health. Thus, rapid and sensitive quantification is highly necessary. Herein, an eco-friendly fluorometric and electrochemical sensing of TA was developed based on a dysprosium(III)-metal-organic framework (Dy(III)-MOF). An aqueous dispersion of Dy(III)-MOF exhibits strong dual emissions at 479 and 572 nm with an excitation at 272 nm, due to the 4f-4f electronic transition and "antenna effect". Chromophore site of the functional ligand, and Dy(III) ion could potentially serve as a sensing probe for TA via quenching (fluorescence). The fluorometric sensor worked well in a wide linear range concentrations from 0.02 to 25 μM with a limit of detection (LOD) of 0.0053 μM. Secondly, the cyclic voltammetric of TA at Dy(III)-MOF modified screen-printed carbon electrode (SPCE) has been investigated. The Dy(III)-MOF/SPCE showed an anodic peak signal at +0.22 V with a five-fold stronger current than the control electrode surface. Under optimized sensing parameters, the Dy(III)-MOF/SPCE delivered wide linear concentrations from 0.01 to 200 μM with a LOD of 0.0023 μM (S/N = 3). Accessibility of real practical samples in alcoholic and juice-based beverages were quantified, resulting in superior recovery rates (98.13-99.53%), F-test, and t-test confirmed high reliability (<95% confidence level (n = 3)). Finally, practicability result of the electrochemical method was validated by fluorometric with a relative standard deviation (RSD) of 0.18-0.46 ± 0.17% (n = 3). The designed probe has proven to be a key candidate for the accurate analysis of TA in beverage samples to ensure food quality.
    MeSH terms: Beverages; Carbon; Fluorescent Dyes; Fruit*; Tannins; Water*; Reproducibility of Results
  6. Oyewusi HA, Adedamola Akinyede K, Wahab RA, Susanti E, Syed Yaacob SN, Huyop F
    J Biomol Struct Dyn, 2023 Jul 16.
    PMID: 37455463 DOI: 10.1080/07391102.2023.2234040
    The presence of synthetic dyes in water bodies and soil is one of the major issues affecting the global ecology, possibly impacting societal well-being adversely due to the colorants' recalcitrance and toxicity. Herein, the study spectrophotometrically monitored the ability of the Bacillus megaterium H2 azoreductase (AzrBmH2) to degrade four synthetic dyes, reactive blue 4, remazol brilliant red, thymol blue, and methyl red, followed by in-silico assessment using GROMACS. We found that the bacterium degraded as much as 60% of all four synthetic dyes at various tested concentrations. The genome analysis revealed five different azoreductase genes, which were then modeled into the AzrBmH21, AzrBmH22/3, and AzrBmH24/5 templates. The AzrBmH2-substrate complexes showed binding energies with all the dyes of between -10.6 to -6.9 kcal/mol and formed 4-6 hydrogen bonds with the predicted catalytic binding residues (His10, Glu 14, Ser 58, Met 99, Val 107, His 183, Asn184 and Gln 191). In contrast, the lowest binding energies were observed for the AzrBmH21-substrates (-10.6 to -7.9). Molecular dynamic simulations revealed that the AzrBmH21-substrate complexes were more stable (RMSD 0.2-0.25 nm, RMSF 0.05 - 0.3 nm) and implied strong bonding with the dyes. The Molecular Mechanics Poisson-Boltzmann Surface Area results also mirrored this outcome, showing the lowest azoreductase-dye binding energy in the order of AzrBmH21-RB4 (-78.18 ± 8.92 kcal/mol), AzrBmH21-RBR (-67.51 ± 7.74 kcal/mol), AzrBmH21-TB (-46.62 ± 5.23 kcal/mol) and AzrBmH21-MR (-40.78 ± 7.87 kcal/mol). In short, the study demonstrated the ability of the B. megaterium H2 to efficiently decolorize the above-said synthetic dyes, conveying the bacterium's promising use for large-scale dye remediation.Communicated by Ramaswamy H. Sarma.
  7. Lee J, Ismail-Pratt I, Machalek DA, Kumarasamy S, Garland SM
    Prev Med Rep, 2023 Oct;35:102291.
    PMID: 37455756 DOI: 10.1016/j.pmedr.2023.102291
    The COVID-19 pandemic has exacerbated the existing challenges to achieving the WHO target of eliminating cervical cancer as a public health problem by working towards the target of fewer than four cases per 100 000 women. We reviewed the literature to identify potential recovery strategies to support cervical cancer prevention programs in lower-and middle-income countries (LMICs) following COVID-19 disruptions and the extent to which strategies have been implemented. Utilising the WHO health systems framework, we mapped these recovery strategies against the six building blocks to examine their reach across the health system. Most recovery strategies were focused on service delivery, while leadership and governance played a pivotal role in the continuity of cervical cancer prevention programs during the pandemic. Leadership and governance were the drivers for outcomes in the building blocks of health information systems, financing and critical support in operationalising service delivery strategies. In the aftermath of the COVID-19 pandemic with strained health resources and economies, stakeholders would significantly influence the coverage and sustainability of cervical cancer prevention programs. The support from multisectoral stakeholders would accelerate the recovery of cervical cancer prevention programs. To achieve the WHO target by 2030, we call for future studies to understand the barriers and facilitators from the perspectives of stakeholders in order to support the decision-making processes and information required to implement recovery strategies in LMICs.
  8. Sureshkumar S, Mwangi KJ, Gathecha G, Marcus K, Kohlbrenner B, Issom D, et al.
    Pan Afr Med J, 2023;44:153.
    PMID: 37455892 DOI: 10.11604/pamj.2023.44.153.38616
    INTRODUCTION: over one third of total Disability-Adjusted-Life-Years lost in Kenya are due to non-communicable diseases (NCD). In response, the Government declared significant commitment towards improving NCD care. The COVID-19 pandemic increased the burden on the already overstretched health systems in Kenya. The aims of this study are to assess whether health care providers perceived NCD care to be optimal during the pandemic and explore how to improve responses to future emergencies.

    METHODS: this cross-sectional online survey included healthcare personnel with non-clinical roles (public health workers and policy-makers) and those delivering health care (doctors and nurses). Respondents were recruited between May and September 2021 by random sampling, completed by snowball sampling.

    RESULTS: among 236 participants (42% in clinical, 58% in non-clinical roles) there was an overall consensus between respondents on NCD care being disrupted and compromised during the pandemic in Kenya. Detracted supplies, funding, and technical resources affected the continuity of NCDs' response, despite government efforts. Respondents agreed that the enhanced personnel capacity and competencies to manage COVID-19 patients were positive, but noted a lack of guidance for redirecting care for chronic diseases, and advocated for digital innovation as a solution.

    CONCLUSION: this paper explores the perceptions of key stakeholders involved in the management of NCDs in Kenya to improve planning for future emergency responses. Gaps were identified in health system response and preparedness capacity during the pandemic including the perceived need to strengthen NCD services, with solutions offered to guide resilience efforts to protect the health system from disruption.

    MeSH terms: Cross-Sectional Studies; Humans; Kenya; Pandemics
  9. Karunyam BV, Abdul Karim AK, Naina Mohamed I, Ugusman A, Mohamed WMY, Faizal AM, et al.
    Front Endocrinol (Lausanne), 2023;14:1147306.
    PMID: 37455908 DOI: 10.3389/fendo.2023.1147306
    INTRODUCTION: Stress and infertility form a complex relationship. In line with this, various stress-related biological markers have been investigated in infertility.

    METHODS: This systematic review was performed using PRISMA guidelines (i) to report whether cortisol is highly present in infertile patients compared to fertile control; (ii) to report whether there is any significant difference in the cortisol level in infertile subjects that conceive and those that didn't at the end of assisted reproduction treatments. Original articles involving human (male and female) as subjects were extracted from four electronic databases, including the list of references from the published papers. Sixteen original full-length articles involving male (4), female (11), and both genders (1) were included.

    RESULTS: Findings from studies that compared the cortisol level between infertile and fertile subjects indicate that (i) Male: three studies reported elevated cortisol level in infertile patients and one found no significant difference; (ii) Female: four studies reported increased cortisol level in infertile subjects and three studies found no significant difference. Findings from studies that measured the cortisol level from infertile patients that conceived and those that didn't indicate that (i) Male: one study reported no significant difference; (ii) Female: one study reported elevated cortisol in infertile patients that conceived, whereas two studies reported increased cortisol in infertile patients that was unable to conceive. Five studies found no significant difference between the groups.

    DISCUSSION: In the present review we only included the cortisol value that was measured prior to stimulation or IVF treatment or during natural or spontaneous cycles, despite this, there are still variations in the sampling period, assessment techniques and patients' characteristics. Hence, at present, we are still unable to conclude that cortisol is significantly elevated in infertile patients. We warrant future studies to standardize the time of biological sample collection and other limitations that were addressed in the review to negate the unwanted influencing factors.

    MeSH terms: Female; Fertility; Fertilization; Humans; Hydrocortisone*; Infertility*; Male
  10. Ahmad MF, Elias MH, Mat Jin N, Abu MA, Syafruddin SE, Zainuddin AA, et al.
    Front Endocrinol (Lausanne), 2023;14:1192180.
    PMID: 37455921 DOI: 10.3389/fendo.2023.1192180
    In vitro oocyte maturation (IVM) has been used worldwide. Despite the long-term implementation, the uptake of this procedure to complement current in vitro fertilization (IVF) remains low. The main reason is likely due to the non-synchronization of protocol and definition criteria, leading to difficulty in collective proper outcome data worldwide and, thus, lack of understanding of the exact IVM procedure. The review aims to consolidate the current clinical practice of IVM by dissecting relevant publications to be tailored for a current spectrum of clinical practice. Nevertheless, the background theories of oocyte maturation were also explored to provide a comprehensive understanding of the basis of IVM theories. Additional discussion of other potential uses of IVM in the future, such as in ovarian tissue cryopreservation known as OTO-IVM for fertility preservation and among women with diminished ovarian reserve, was also explored. Otherwise, future collaboration among all IVM centers is paramount for better collection of clinical data to provide valid recommendations for IVM in clinical practice, especially in molecular integrity and possible DNA alteration if present for IVM offspring outcome safety purposes.
  11. Adli Zakaria MN, Ahmed AN, Abdul Malek M, Birima AH, Hayet Khan MM, Sherif M, et al.
    Heliyon, 2023 Jul;9(7):e17689.
    PMID: 37456046 DOI: 10.1016/j.heliyon.2023.e17689
    Accurate water level prediction for both lake and river is essential for flood warning and freshwater resource management. In this study, three machine learning algorithms: multi-layer perceptron neural network (MLP-NN), long short-term memory neural network (LSTM) and extreme gradient boosting XGBoost were applied to develop water level forecasting models in Muda River, Malaysia. The models were developed using limited amount of daily water level and meteorological data from 2016 to 2018. Different input scenarios were tested to investigate the performance of the models. The results of the evaluation showed that the MLP model outperformed both the LSTM and the XGBoost models in predicting water levels, with an overall accuracy score of 0.871 compared to 0.865 for LSTM and 0.831 for XGBoost. No noticeable improvement has been achieved after incorporating meteorological data into the models. Even though the lowest reported performance was reported by the XGBoost, it is the faster of the three algorithms due to its advanced parallel processing capabilities and distributed computing architecture. In terms of different time horizons, the LSTM model was found to be more accurate than the MLP and XGBoost model when predicting 7 days ahead, demonstrating its superiority in capturing long-term dependencies. Therefore, it can be concluded that each ML model has its own merits and weaknesses, and the performance of different ML models differs on each case because these models depends largely on the quantity and quality of data available for the model training.
  12. Akter SN, Bi S, Qiu X, Islam Sarker MN
    Heliyon, 2023 Jul;9(7):e17549.
    PMID: 37456053 DOI: 10.1016/j.heliyon.2023.e17549
    This study provides an alternative agenda to better explain the Belt and Road Initiative's (BRI's) technological connotations in Bangladesh using the Game Theory and Demand Curve approaches. BRI can proceed as a means to technology development for Bangladesh based on foreign direct investment (FDI) spillover effects that ranked China as the top FDI source, with 1159.42 million USD invested in 2018-2019. The findings suggest that motivated by mutual interests of economic transformation (China) and technological requirements (Bangladesh), BRI offers a bargaining game of cooperation. Thus, while economic transformation may force China to relocate its garment factories, Bangladesh's low wages and geopolitical location give it a superior position regarding relocation. The technological effects of such relocation will be two-fold: exchanges of tacit knowledge (conventional) and techno-based infrastructural support (component) that align with the proposed technology development framework on a macro level. More conventional technological projects and additional sector-based technology transfer are required to amplify BRI's technological forecasts. Moreover, to encourage more abundant FDI, bank loan interest must be decreased, and political stability has to be ensured. Both survey-based fieldwork and projects-based qualitative research need to be conducted to discover BRI's tangible technological implications.
  13. Chung PY, Loh PLN, Neoh HM, Ramli R
    Heliyon, 2023 Jul;9(7):e17892.
    PMID: 37456062 DOI: 10.1016/j.heliyon.2023.e17892
    Staphylococcus aureus has caused life-threatening infections and developed resistance against conventional antimicrobials, posing a significant threat to human health worldwide. Biofilms that surround the bacteria cells act as a protective layer, allowing cells inside the biofilm to be resistant to external stresses such as antimicrobials. Therefore, biofilms further complicate treatment available for infections caused by multi-drug resistant Staphylococcus aureus. A previous study on alpha-amyrin (AM), derived from ursane, was reported to significantly reduce the biomass and inhibit the metabolic activity of reference strain methicillin-resistant and methicillin-sensitive S. aureus (MRSA and MSSA, respectively). In this study, the antibiofilm activity of AM was extended to include clinical isolates of MSSA and MRSA, and laboratory-generated vancomycin-intermediate S. aureus (VISA) collected from University Kebangsaan Malaysia Medical Center (PPUKM) and Universiti Kebangsaan Malaysia Medical Molecular Biology Institute (UMBI). Pre-formed biofilms of biofilm-forming isolates identified from the Congo Red Agar (CRA) assay were then exposed to AM, vancomycin and oxacillin, and evaluated using the crystal violet and resazurin assays. The results showed that AM reduced the biofilm biomass of three isolates of MSSA, eight isolates of MRSA and four isolates of VISA but increased the metabolic activity in certain MSSA, MRSA and VISA isolates, indicating AM may possess biofilm reduction effects but not bactericidal effects. Based on these findings, AM could be further studied and developed as a potential therapeutic agent for chronic S. aureus infections.
  14. Suppiah S, Mohd Rohani MF, Zanial AZ, Ahmad Shahrir AD, Khairuman KA, Vinjamuri S
    Indian J Nucl Med, 2023;38(2):191-200.
    PMID: 37456181 DOI: 10.4103/ijnm.ijnm_142_22
    Planar whole-body bone scanning (WBS) is widely used to evaluate skeletal lesions seen in cancer and noncancer cases. Frequently, degenerative, or other benign bony changes may give rise to indeterminate lesions that mimic bone metastases. In the post-COVID-19 era, there is an evolutionary phase that puts importance on global development and adaptability, which encompasses to include nuclear medicine practices worldwide. Single-photon emission computed tomography/computed tomography (SPECT/CT) can be used to improve the characterization of these lesions and help to resolve the diagnostic conundrum while reducing the need for patients to undergo multiple different examinations at various imaging departments. The fusion of SPECT and CT allows morphological characterization of functional abnormality detected by focal tracer uptake on planar scintigraphy, which provides a one-stop center imaging in nuclear medicine departments. The objective of this study was to review the diagnostic accuracy of SPECT/CT in diagnosing bone metastases in a variety of oncology and nononcology cases and to determine the feasibility of performing bone SPECT/CT in all suspected cancer cases, including cases of bone infection instead of planar imaging alone. The utilization of hybrid SPECT/CT in indeterminate bone lesions detected on planar WBS can significantly increase the diagnostic confidence and accuracy of image interpretation. Recognition of patterns of disease identified using hybrid imaging can improve the management of patients with potentially lower costs in the long term. Currently, hybrid SPECT/CT machines are becoming a norm in nuclear medicine departments, thus potentially making single planar application machines obsolete in the near future. We hypothesize that in the interest of providing a meaningful interpretation of isotope bone scans, the default protocol should involve the option of acquiring SPECT/CT images rather than relying on whole-body scans only. Departments choosing to upgrade existing equipment or those choosing to invest in only one gamma camera should proactively opt for hybrid SPECT/CT systems.
  15. Anwarali Khan MH, Kow RY, Ramalingam S, Sofian A, Ho JPY, Jaharan Singh KS, et al.
    Cureus, 2023 Jun;15(6):e40479.
    PMID: 37456372 DOI: 10.7759/cureus.40479
    Introduction With the advancing age of the population, there are an increasing number of patients with geriatric hip fractures. Despite the advancement of surgical knowledge and improvement of implant designs to treat geriatric hip fractures, mortality and morbidity remain high among these frail patients. In conjunction with the COVID-19 pandemic, the collateral damage dealt to these patients remains unknown as scarce resources are funneled to deal with the pandemic. This study is geared to investigate the surgical outcomes of patients with geriatric hip fractures who were admitted during the initial phase of the COVID-19 pandemic. Methods This retrospective study was carried out at Hospital Kuala Lumpur, the largest public hospital in the capital of Malaysia, from March 1, 2020, to March 1, 2021. All patients of age 60 years and above were screened for suitability. Only patients who had undergone surgical intervention during the study period were included in this study. Patients' demographic data, mechanism of injury, waiting time for surgery, type of surgery, complications and ambulatory status were obtained from the medical records. Univariate analysis was performed to determine the factors associated with complications as well as the post-operative ambulatory status of the patients. Results A total of 52 patients were included in this study, with a median age of 72 years. The majority of the patients were Chinese (n=21, 40.4%). This was followed by Malay and Indian (n=14, 26.9% each) and other ethnicity (n=3, 5.8%). More than three-quarters of the patients had a trivial injury such as a fall due to a miss-step (n=16, 30.8%) and slip (n=16, 30.8%) and a fall due to dizziness (n=8, 15.4%). Only 12 patients (23.1%) sustained hip fractures due to trauma. The median time to surgery for these patients was 5 days (interquartile range: 4 days). Most of these patients underwent total hip replacement (n=30, 57.7%). This was followed by unipolar hemiarthroplasty (n=11, 21.2%), bipolar hemiarthroplasty (n=10, 19.2%) and internal fixation (n=1, 1.9%). Among these patients, six of them had documented complications. There were periprosthetic joint infection (n=2, 3.8%), dislocation (n=2, 3.8%), hematoma formation (n=1, 1.9%) and seroma (n=1, 1.9%). Six months after the surgery, most of the patients were able to ambulate, albeit some patients required walking aid such as walking stick and walking frame. Univariate analysis showed that all the factors were not associated with the complications and the post-operative ambulatory status of the patients. Conclusion  The incidence of geriatric hip fractures remains high during the COVID-19 pandemic despite the movement control order (MCO) being enforced in Malaysia. With prompt surgical intervention, most of the patients can regain ambulatory status, albeit with a walking aid.
  16. Dewi R, Yusoff NA, Abdul Razak SR, Abd Hamid Z
    PeerJ, 2023;11:e15608.
    PMID: 37456886 DOI: 10.7717/peerj.15608
    BACKGROUND: HSPCs are targets for benzene-induced hematotoxicity and leukemogenesis. However, benzene toxicity targeting microRNAs (miRNAs) and transcription factors (TF) that are involve in regulating self-renewing and differentiation of HSPCs comprising of different hematopoietic lineages remains poorly understood. In this study, the effect of a benzene metabolite, 1,4-benzoquinone (1,4-BQ) exposure, in HSPCs focusing on the self-renewing (miRNAs: miR-196b and miR-29a; TF: HoxB4, Bmi-1) and differentiation (miRNAs: miR-181a, TF: GATA3) pathways were investigated.

    METHODS: Freshly isolated mouse BM cells were initially exposed to 1,4-BQ at 1.25 to 5 µM for 24 h, followed by miRNAs and TF studies in BM cells. Then, the miRNAs expression was further evaluated in HSPCs of different lineages comprised of myeloid, erythroid and pre-B lymphoid progenitors following 7-14 days of colony forming unit (CFU) assay.

    RESULTS: Exposure to 1,4-BQ in BM cells significantly (p 

    MeSH terms: Animals; Benzene/toxicity; Cell Differentiation/genetics; Hematopoietic Stem Cells; Transcription Factors/genetics; Benzoquinones/toxicity; Mice
  17. Shahroom A, Shapawi R, Mustafa S, Abd Halid NF, Estim A, Daning Tuzan A
    PeerJ, 2023;11:e15449.
    PMID: 37456888 DOI: 10.7717/peerj.15449
    The growth performance of red claw crayfish, Cherax quadricarinatus, fed diets with different sources and concentrations of natural calcium was evaluated. Formulated diets containing 30% protein and 8% lipid were prepared using supplementation of 0 (control), 3%, 6%, 9% eggshell, and 6% seashell. C. quadricarinatus juveniles with an average total weight of 0.21 ± 0.01 g and length of 25.0 ± 0.10 mm were obtained from ten ovigerous females bred in captivity. An aquarium with a size of 0.72 m² was divided into eight compartments with an area of 0.09 m² per compartment and was individually stocked with juvenile crayfish. A total of eight juvenile crayfish were used per dietary treatment. There were five dietary treatments in total and each of these treatments were replicated three times. The addition of eggshell and seashell powder exerted positive effects on the growth performance, molting frequency, and survival of the crayfish. Crayfish fed with a 6% eggshell-supplemented diet exhibited the best overall growth performance. The calcium percentage of the carapace increased with the increase in calcium content of the diets. Meanwhile, the performance of diets comprising eggshells and seashells was not significantly different (P > 0.05). Considering the low cost and abundance of eggshells, the addition of 6% of this product to the diet is recommended for the best growth of juvenile C. quadricarinatus.
    MeSH terms: Animals; Calcium, Dietary; Astacoidea*; Egg Shell; Powders; Molting*; Dietary Supplements; Animal Shells
  18. Zakaria MH, Ramaiya SD, Bidin N, Syed NNF, Bujang JS
    PeerJ, 2023;11:e15496.
    PMID: 37456903 DOI: 10.7717/peerj.15496
    BACKGROUND: The social acceptability of wild freshwater macrophytes as locally consumed vegetables is widespread. Freshwater macrophytes have several uses; for example, they can be used as food for humans. This study determined the proximate composition and mineral content of three freshwater macrophyte species, i.e., Eichhornia crassipes, Limnocharis flava, and Neptunia oleracea.

    METHODS: Young shoots of E. crassipes, L. flava, and N. oleracea were collected from shallow channels of Puchong (3°00'11.89″N, 101°42'43.12″E), Ladang 10, Universiti Putra Malaysia (2°58'44.41″N, 101°42'44.45″E), and Kampung Alur Selibong, Langgar (06°5'50.9″N, 100°26'49.8″E), Kedah, Peninsular Malaysia. The nutritional values of these macrophytes were analysed by using a standard protocol from the Association of Official Analytical Chemists. Eight replicates of E. crassipes and L. flava and four replicates of N. oleracea were used for the subsequent analyses.

    RESULTS: In the proximate analysis, N. oleracea possessed the highest percentage of crude protein (29.61%) and energy content (4,269.65 cal g-1), whereas L. flava had the highest percentage of crude fat (5.75%) and ash (18.31%). The proximate composition trend for each species was different; specifically, all of the species possessed more carbohydrates and fewer crude lipids. All of the species demonstrated a similar mineral trend, with high nitrogen and potassium and lower copper contents. Nitrogen and potassium levels ranged from 12,380-40,380 mg kg-1 and from 11,212-33,276 mg kg-1, respectively, and copper levels ranged from 16-27 mg kg-1. The results showed that all three plant species, i.e., E. crassipes, N. oleracea, and L. flava are plant-based sources of macro- and micronutrient beneficial supplements for human consumption.

    MeSH terms: Fresh Water/analysis; Humans; Nitrogen/analysis; Nutritive Value; Potassium/analysis; Vegetables
  19. Salem HMA, Chok KC, Koh RY, Ng PY, Tiong YL, Chye SM
    Int J Biochem Mol Biol, 2023;14(3):25-31.
    PMID: 37456910
    Diabetic neuropathy (DN) is a condition in which nerve fibers are continually exposed to high glucose-induced free radicals. Recent discoveries demonstrated that melatonin is an indole hormone that contributes to neuroprotection through the modulation of autophagy. Herein, this study aims to examine the neuroprotective effects of melatonin on Schwann cells under high glucose conditions. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay was used to measure cell viability. The activation of autophagosomes was determined using acridine orange staining (AO). Western blot assay was used to measure the expression of proteins involved in autophagy and endoplasmic reticulum (ER) stress. Our results demonstrated that melatonin at 1 µM has the highest protective effects on high glucose-induced cell death. Melatonin concentrations of 5 and 10 µM were found to be the most effective in reducing autophagy induced by high glucose. Under high glucose conditions, the protein expressions of LC3, ATF4, ATF6, CHOP, PERK and eIF2-α were up-regulated in Schwann cells. However, melatonin attenuated these changes by downregulating LC3 and the ER stress markers ATF4, ATF6, CHOP, PERK and eIF2-α protein expressions in Schwann cells. In conclusion, melatonin alleviates high glucose-induced autophagy in Schwann cells through PERK-eIF2α-ATF4-CHOP signaling pathways.
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