Browse publications by year: 2024

Intelligent mutation based evolutionary optimization algorithm for genomics and precision medicine

Singh SP, Yadav DK, Chamran MK, Perera DG

Funct Integr Genomics, 2024 Jul 22;24(4):128.
PMID: 39037544 DOI: 10.1007/s10142-024-01401-3

In this paper, genomics and precision medicine have witnessed remarkable progress with the advent of high-throughput sequencing technologies and advances in data analytics. However, because of the data's great dimensionality and complexity, the processing and interpretation of large-scale genomic data present major challenges. In order to overcome these difficulties, this research suggests a novel Intelligent Mutation-Based Evolutionary Optimization Algorithm (IMBOA) created particularly for applications in genomics and precision medicine. In the proposed IMBOA, the mutation operator is guided by genome-based information, allowing for the introduction of variants in candidate solutions that are consistent with known biological processes. The algorithm's combination of Differential Evolution with this intelligent mutation mechanism enables effective exploration and exploitation of the solution space. Applying a domain-specific fitness function, the system evaluates potential solutions for each generation based on genomic correctness and fitness. The fitness function directs the search toward ideal solutions that achieve the problem's objectives, while the genome accuracy measure assures that the solutions have physiologically relevant genomic properties. This work demonstrates extensive tests on diverse genomics datasets, including genotype-phenotype association studies and predictive modeling tasks in precision medicine, to verify the accuracy of the proposed approach. The results demonstrate that, in terms of precision, convergence rate, mean error, standard deviation, prediction, and fitness cost of physiologically important genomic biomarkers, the IMBOA consistently outperforms other cutting-edge optimization methods.

MeSH terms: Algorithms*; Humans; Mutation*; Evolution, Molecular
Markov chain quasi-Monte Carlo method for forecasting fire hotspots in Sarawak, Malaysia

Zakaria NN, Daud H, Sokkalingam R, Othman M, Abdul Kadir E, Mohd Aris MN, et al.

Environ Sci Pollut Res Int, 2024 Jul 22.
PMID: 39037622 DOI: 10.1007/s11356-024-34409-0

Stochastic modeling approaches have attracted many researchers to the field. However, fire hotspot detection suffers from not using a Markov chain quasi-Monte Carlo (MCQMC) as a forecasting methodology. This paper proposes improvements to the computational time by combining the strengths of the Markov chain Monte Carlo (MCMC) and quasi-Monte Carlo (QMC) methods. The proposed method can lead to more precise and stable results, particularly in problems with high-dimensional integration or complex probability distributions. The proposed method is applied to a case study of fire hotspot detection in Sarawak, Malaysia. The outcome of this study reveals that the MCQMC method is more computationally efficient, taking only 0.0746 seconds compared to MCMC's 0.0914 seconds and QMC's 0.0994 seconds. It is shown that the best option derived by the proposed method is effective in predicting fire hotspots and providing quick solutions to protect the environment and communities from forest fires.
Peperomia pellucida (L.) Kunth suppresses glycation-induced inflammatory response in human retinal pigment epithelial cell line ARPE-19 via JAK-STAT3 signaling

Ho KL, Yong PH, Lim SH, Ng ZX

Arch Pharm (Weinheim), 2024 Jul 22.
PMID: 39037823 DOI: 10.1002/ardp.202400299

The formation of advanced glycation end product (AGE) is a risk factor for diabetic retinopathy. Since the current treatment for diabetic retinopathy is accompanied by side effects, preliminary findings have suggested Peperomia pellucida (L.) Kunth as a potential alternative therapeutic option for diabetic retinopathy. This study aimed to elucidate the anti-inflammatory mechanism of P. pellucida in the AGE-stimulated human retinal pigment epithelial cell line ARPE-19. Phytochemical analysis revealed phenylpronanoids, terpenes, and fatty acids in P. pellucida. Through in vitro cell viability assay, the P. pellucida methanolic extract (IC50 = 8.70 mg/mL) and ethyl acetate fraction (IC50 = 7.34 mg/mL) were considered as non toxic for ARPE-19. AGE induced an inflammatory response in ARPE-19 by upregulating the gene (2.4-5.8-fold) and protein (1.4-2.3-fold) expression of signal transducer and activator of transcription 3 (STAT3), interleukin-8 (IL-8), monocyte chemoattractant protein-1, matrix metalloproteinase 2, and vascular endothelial growth factor. At 1.5 mg/mL, P. pellucida methanolic extract suppressed IL-8 expression (p
Fulltext The association between HIV-related stigma, HIV knowledge and HIV late presenters among people living with HIV (PLHIV) attending public primary care clinic settings in Selangor

Bin Ahmad MZ, Md Yasin M, Mat Nasir N, Mohamad M

PLoS One, 2024;19(7):e0306904.
PMID: 39037975 DOI: 10.1371/journal.pone.0306904

INTRODUCTION: HIV late presenters were defined as individuals presenting with a CD4 count below 350 cells/μL or with an AIDS-defining event, according to the European Late Presenter Consensus working group. Early diagnosis and treatment of HIV have proven beneficial for people living with HIV (PLHIV), reducing the burden on healthcare systems, and contributing to ending the HIV/AIDS epidemic. However, in Malaysia, over 50% of newly diagnosed HIV patients present late, leading to increased morbidity and premature mortality. This study aims to determine the prevalence of late HIV presenters and its association with HIV-related stigma and HIV knowledge among PLHIV attending public primary care clinics in Selangor.
METHODS: A cross-sectional study was conducted at selected public health clinics in Selangor, involving PLHIV aged 18 years and older, who were diagnosed since 2019. HIV-related stigma was measured using the Malay version of Berger's HIV Stigma Scale, and HIV knowledge was assessed using the Malay version of Brief HIV-KQ-18. Univariate and multivariate logistic regression analyses were performed to identify factors associated with late HIV presentation.
RESULTS: A total of 400 participants were included in the study, with 60.0% (n = 240, 95% CI: 55.0-65.0) classified as late presenters. The participants had a mean age of 30.29 (±7.77) years. The risk factors for late presenters were high levels of HIV-related stigma (aOR = 1.049, 95% CI: 1.034-1.063, p-value <0.001), low levels of HIV knowledge (aOR = 0.709, 95% CI: 0.646-0.778, p-value <0.001), tertiary education background (aOR = 15.962, 95% CI: 1.898-134.235, p-value = 0.011), and being single (aOR = 3.582, 95% CI: 1.393-9.208, p-value = 0.008).
CONCLUSION: This study highlights the association between high levels of HIV-related stigma, low levels of HIV knowledge, and late HIV presentation. Interventions targeting stigma reduction and HIV education can promote early testing and prompt access to care, improving health outcomes for PLHIV.

MeSH terms: Adult; Cross-Sectional Studies; Female; Humans; Health Knowledge, Attitudes, Practice*; Malaysia/epidemiology; Male; Middle Aged; Primary Health Care*; Young Adult; Social Stigma*
Leptin in reproduction and hypertension in pregnancy

Singh H, Almabhouh FA, Alshaikhli HSI, Hassan MJM, Daud S, Othman R, et al.

Reprod Fertil Dev, 2024 Jul;36.
PMID: 39038160 DOI: 10.1071/RD24060

Leptin has important roles in numerous physiological functions, including those in the regulation of energy balance, and in immune and reproductive systems. However, in the recent years, evidence has implicated it in a number of obesity-related diseases, where its concentrations in serum are significantly elevated. Elevated serum leptin concentrations and increased placental leptin secretion have been reported in women with hypertensive disorders of pregnancy. Whether leptin is responsible for this disorder remains to be established. Leptin injections in healthy rats and mice during pregnancy result in endothelial activation, increased blood pressure and proteinuria. A potential role for leptin in the pathogenesis of pre-eclampsia is hypothesised, particularly in women who are overweight or obese where serum leptin concentrations are often elevated. This review summarises pertinent information in the literature on the role of leptin in puberty, pregnancy, and hypertensive disorders of pregnancy. In particular, the possible mechanism that may be involved in leptin-induced increase in blood pressure and proteinuria during pregnancy and the potential role of marinobufagenin in this disease entity. We hypothesise a significant role for oxidative stress in this, and propose a conceptual framework on the events that lead to endothelial activation, raised blood pressure and proteinuria following leptin administration.

MeSH terms: Animals; Blood Pressure/physiology; Female; Humans; Hypertension/metabolism; Hypertension/physiopathology; Pre-Eclampsia/metabolism; Pre-Eclampsia/physiopathology; Pregnancy; Reproduction/physiology; Hypertension, Pregnancy-Induced/metabolism; Hypertension, Pregnancy-Induced/physiopathology
Fouling mitigation of microcrystalline cellulose-blended poly(vinylidene fluoride) membranes in protein separation

Che Lah NF, Ahmad AL, Nazri AI, Zaulkiflee ND

Environ Technol, 2024 Jul 22.
PMID: 39038428 DOI: 10.1080/09593330.2024.2380394

Modifications of polymeric membranes are necessary to improve the membrane characteristics. The composite membrane of Poly(vinylidene fluoride)_Microcrystalline cellulose (PVDF_MCC) was prepared using dimethylacetamide/lithium chloride (DMAc/LiCl) as co-solvent to dissolve the MCC in the casting solution. The prepared membrane was characterized by using the FTIR, SEM, contact angle, and the water permeates flux. The improvement of hydrophilicity and pore structure with the incorporation of MCC was observed which led to the increment of the water and permeate flux. Indication from the rheological study suggested that the casting solutions with MCC presented the influence on the composite membrane's pore structure as a significant viscosity increment was observed. This can be examined from the larger pore and elongated finger-like structure of the membrane morphology compared to the pristine membrane. The rejection rate of the protein increased from 85% to 97% after the incorporation of MCC in the PVDF membrane. The utilization of a low-cost and environmentally friendly additive that MCC has to offer helps to improve the antifouling properties of PVDF membranes for the efficient removal of BSA in water.
Determinants of the utilization of recognized traditional and complementary medicine service in Malaysia: a secondary analysis of a cross-sectional population-based survey

Ng SK, Nishino K, Seman Z, Inthaphatha S, Yamamoto E

Complement Med Res, 2024 Jul 22.
PMID: 39038440 DOI: 10.1159/000540114

INTRODUCTION: The utilization of traditional and complementary medicine (T&CM) services has witnessed a global increase over the past decades. Currently, seven practice areas are recognized in Malaysia: traditional Malay medicine, traditional Chinese medicine, traditional Indian medicine, homeopathy, Islamic medical practice, chiropractic, and osteopathy. Many global studies have investigated the general determinants of T&CM service utilization. However, there has been no comprehensive study reporting specific determinants of recognized T&CM service utilization. This study aimed to examine the prevalence and specific determinants of recognized T&CM service utilization in Malaysia at a national level.
METHODS: This study is a secondary analysis of data from the National Health and Morbidity Survey (NHMS), a cross-sectional population-based survey conducted in Malaysia in 2015. A total of 6,207 respondents aged ≥18 years were included in this study. The associations of sociodemographic, health, and lifestyle factors with the utilization of the three T&CM service categories (traditional Malay medicine, traditional Chinese medicine, and other T&CM) were examined using three separate logistic regression analyses.
RESULTS: The prevalence of recognized T&CM service utilization in the last 12 months was 19.3%, with 15.0% for traditional Malay medicine, 3.9% for traditional Chinese medicine, and 1.5% for other T&CM. The determinants of traditional Malay medicine service utilization were female sex; age range of 18-29 years; married or widowed/divorced status; Malay, Indigenous or other ethnicities; high household income; and being overweight/obese. The determinants of traditional Chinese medicine service utilization were the age range of 30-49 years, urban residential location, Chinese ethnicity, and adequate fruit and vegetable intake. No specific determinants were identified for other T&CM.
CONCLUSION: This study provided novel evidence of a strong ethnocultural ownership towards traditional medicine. User profiles were distinctively different between varied T&CM services. Customized approaches to regulate, develop, and institutionalize specific T&CM services are crucial for fulfilling the unique needs of diverse communities.
Efficient and easible biocatalysts: Strategies for enzyme improvement. A review

Albayati SH, Nezhad NG, Taki AG, Rahman RNZRA

Int J Biol Macromol, 2024 Sep;276(Pt 2):133978.
PMID: 39038570 DOI: 10.1016/j.ijbiomac.2024.133978

Owing to the environmental friendliness and vast advantages that enzymes offer in the biotechnology and industry fields, biocatalysts are a prolific investigation field. However, the low catalytic activity, stability, and specific selectivity of the enzyme limit the range of the reaction enzymes involved in. A comprehensive understanding of the protein structure and dynamics in terms of molecular details enables us to tackle these limitations effectively and enhance the catalytic activity by enzyme engineering or modifying the supports and solvents. Along with different strategies including computational, enzyme engineering based on DNA recombination, enzyme immobilization, additives, chemical modification, and physicochemical modification approaches can be promising for the wide spread of industrial enzyme usage. This is attributed to the successful application of biocatalysts in industrial and synthetic processes requires a system that exhibits stability, activity, and reusability in a continuous flow process, thereby reducing the production cost. The main goal of this review is to display relevant approaches for improving enzyme characteristics to overcome their industrial application.

MeSH terms: Biotechnology/methods; Enzyme Stability; Protein Engineering/methods; Biocatalysis*
Investigating process parameters to enhance (hemi)cellulolytic enzymes activity produced by Trichoderma reesei RUT-C30 using deoiled oil palm mesocarp fiber in solid-state fermentation

Al-Qassab AA, Zakaria MR, Yunus R, Salleh MAM, Mokhtar MN

Int J Biol Macromol, 2024 Sep;276(Pt 2):134030.
PMID: 39038578 DOI: 10.1016/j.ijbiomac.2024.134030

This study investigates the synthesis of (hemi)cellulolytic enzymes, including endoglucanase (CMCase), xylanase, and β-glucosidase, employing Trichoderma reesei RUT-C30 and deoiled oil palm mesocarp fiber (OPMF) through solid-state fermentation (SSF). The objective was to determine the optimal process conditions for achieving high enzyme activities through a one-factor-at-a-time approach. The study primarily focused on the impact of the solid-to-liquid ratio, incubation period, initial pH, and temperature on enzyme activity. The effects of OPMF pretreatment, particularly deoiling and fortification, were explored. This approach significantly improved enzyme activity levels compared to the initial conditions, with CMCase increasing by 111.6 %, xylanase by 665.2 %, and β-Glucosidase by 1678.1 %. Xylanase and β-glucosidase activities, peaking at 1346.75 and 9.89 IU per gram dry substrate (GDS), respectively, under optimized conditions (1:4 ratio, pH 7.5, 20 °C, 9-day incubation). With lower moisture levels, CMCase reached its maximum activity of 227.84 IU/GDS. The study highlights how important it is for agro-industrial byproducts to support environmentally sustainable practices in the palm oil industry. It also emphasizes how differently each enzyme reacts to changes in process parameters.

MeSH terms: beta-Glucosidase/metabolism; Cellulase/metabolism; Cellulose/metabolism; Cellulose/chemistry; Fermentation*; Hydrogen-Ion Concentration; Hypocreales/enzymology; Temperature*; Endo-1,4-beta Xylanases/metabolism
Fulltext Accounting for deep soil carbon in tropical forest conservation payments

Sundqvist MK, Hasselquist NJ, Jensen J, Runesson J, Goodman RC, Axelsson EP, et al.

Sci Rep, 2024 Jul 22;14(1):16772.
PMID: 39039098 DOI: 10.1038/s41598-024-65138-6

Secondary tropical forests are at the forefront of deforestation pressures. They store large amounts of carbon, which, if compensated for to avoid net emissions associated with conversion to non-forest uses, may help advance tropical forest conservation. We measured above- and below-ground carbon stocks down to 1 m soil depth across a secondary forest and in oil palm plantations in Malaysia. We calculated net carbon losses when converting secondary forests to oil palm plantations and estimated payments to avoid net emissions arising from land conversion to a 22-year oil palm rotation, based on land opportunity costs per hectare. We explored how estimates would vary between forests by also extracting carbon stock data for primary forest from the literature. When tree and soil carbon was accounted for, payments of US$18-51 tCO2-1 for secondary forests and US$14-40 tCO2-1 for primary forest would equal opportunity costs associated with oil palm plantations per hectare. If detailed assessments of soil carbon were not accounted for, payments to offset opportunity costs would need to be considerably higher for secondary forests (US$28-80 tCO2-1). These results show that assessment of carbon stocks down to 1 m soil depth in tropical forests can substantially influence the estimated value of avoided-emission payments.

MeSH terms: Malaysia; Trees; Tropical Climate*; Forests*
Fulltext Experimental analysis of enhanced finite set model predictive control and direct torque control in SRM drives for torque ripple reduction

M D, Samithas D, Balachandran PK, Selvarajan S

Sci Rep, 2024 Jul 22;14(1):16805.
PMID: 39039123 DOI: 10.1038/s41598-024-65202-1

The magnet-less switched reluctance motor (SRM) speed-torque characteristics are ideally suited for traction motor drive characteristics and its advantage to minimize the overall cost of on-road EVs. The main drawbacks are torque and flux ripple, which have produced high in low-speed operation. However, the emerging direct torque control (DTC) operated magnitude flux and torque estimation with voltage vectors (VVs) gives high torque ripples due to the selection of effective switching states and sector partition accuracy. On the other hand, the existing model predictive control (MPC) with multiple objective and optimization weighting factors produces high torque ripples due to the system dynamics and constraints. Therefore, existing DTC and MPC can result in high torque ripples. This paper proposed a finite set (FS)-MPC with a single cost function objective without weighting factor: the predicted torque considered to evaluate VVs to minimize the ripples further. The selected optimal VV minimizes the SRM drive torque and flux ripples in steady and dynamic state behaviour. The classical DTC and proposed model were developed, and simulation results were verified using MATLAB/Simulink. The proposed model operated in SRM drives experimental results to prove the effective minimization of torque and flux ripples compared to the existing DTC.
Fulltext Resting-state electroencephalography (EEG) microstates of healthy individuals following mild sleep deprivation

Khoo SY, Lai WH, On SH, On YY, Adam BM, Law WC, et al.

Sci Rep, 2024 Jul 22;14(1):16820.
PMID: 39039219 DOI: 10.1038/s41598-024-67902-0

Mild sleep deprivation is widespread in many societies worldwide. Electroencephalography (EEG) microstate analysis provides information on spatial and temporal characteristics of resting brain network, serving as an indicator of neurophysiological activities at rest. This study seeks to investigate potential neural markers in EEG following mild sleep deprivation of a single night using EEG microstate analysis. Six-minute resting EEG was conducted on thirty healthy adults within 6 hours of waking in the morning and after at least 18 h of sleep deprivation. Translated and validated Malay language Karolinska Sleepiness Scale was used to assess the participants' degree of sleepiness. Microstate characteristics analysis was conducted on the final 24 subjects based on four standard microstate maps. Microstate C shows a significant increase in mean duration, coverage and occurrence, while microstate D has significantly higher occurrence after sleep deprivation. This study demonstrates notable changes in resting state EEG microstates following mild sleep deprivation. Present findings deepen our understanding of the brain's spatiotemporal dynamics under this condition and suggest the potential utility of neural markers in this domain as components of composite markers for sleep deprivation.

MeSH terms: Adult; Electroencephalography*; Female; Humans; Male; Young Adult; Healthy Volunteers
Fulltext Solid state synthesis of lead-free Sm-doped Na0.5Bi4.5Ti4O15 ceramics for enhanced dielectric and electrical properties

Rehman F, Ahmad P, Rahman AU, Alharthi AI, Khalid A, Faruque MRI, et al.

Heliyon, 2024 Jul 15;10(13):e33606.
PMID: 39040251 DOI: 10.1016/j.heliyon.2024.e33606

Here we report the synthesis of Sm-doped Na0.5Bi4.5Ti4O15 (Na0.5Sm0.5Bi4Ti4O15) lead-free ceramics via a conventional solid-state technique. Investigations of Na0.5Bi4.5Ti4O15 (NBT) and Na0.5Sm0.5Bi4.5Ti4O15 (NSBT) ceramics were demonstrated in detail to understand the composition-based structure-property of Aurivillius compounds and related functional material. Dielectric properties for frequency and temperature in a wide range were analyzed. The conduction activation energy values of NSBT ceramics are obtained to be 1.40 eV, whereas, the NBT ceramics get the value to be 1.31 eV. At higher temperatures, the conduction activation energy value of NSBT ceramics is 1.32 eV for both frequencies of 100 Hz and 1 kHz, whereas, for NBT compounds, the calculated value is 1.27 eV for both frequencies. The simulation performed on the impedance data for capacitive and resistance elements shows well-fitting curves which indicates a single relaxation behavior in the material. Similarly, the AC-conductivity data were analyzed which gives different conduction processes and relaxation activation energies in the NSBT ceramics.
Fulltext Flow cytometry-based quantitative analysis of cellular protein expression in apoptosis subpopulations: A protocol

Alshehade SA, Almoustafa HA, Alshawsh MA, Chik Z

Heliyon, 2024 Jul 15;10(13):e33665.
PMID: 39040270 DOI: 10.1016/j.heliyon.2024.e33665

Flow cytometry techniques utilizing dual staining with annexin V and propidium iodide (PI) provide a robust method for quantitatively analyzing apoptosis induction. Annexin V binds phosphatidylserine exposed on the outer leaflet of the plasma membrane during early apoptosis, while PI permeates late apoptotic/necrotic cells. Simultaneous staining allows differentiation of viable, early apoptotic, and late apoptotic/necrotic populations. This approach can be enhanced by using fluorochrome-conjugated antibodies to stain specific proteins, enabling the simultaneous tracking of protein expression changes in defined cell subpopulations during apoptosis. This multiparametric approach provides key insights into signaling regulation and the mechanisms underlying the apoptotic response to cytotoxic treatments. Here we present a protocol that combines annexin V-FITC/PI staining with APC-conjugated antibody labeling in MDA-MB-231 breast cancer cells treated with doxorubicin. This protocol enables both the quantitative assessment of apoptosis induction and the tracking of decreased CD44 expression from viable to apoptotic cells. This protocol also provides guidelines for appropriate filter selection, compensation controls, gating strategies, and troubleshooting. This robust protocol holds significant potential for elucidating signaling networks involved in apoptosis and therapeutic resistance across various cellular models.
Fulltext Synthesis and characterization of magnetic molecularly imprinted polymers for the rapid and selective determination of clofazimine in blood plasma samples

Zamruddin NM, Herman H, Asman S, Hasanah AN

Heliyon, 2024 Jul 15;10(13):e33396.
PMID: 39040332 DOI: 10.1016/j.heliyon.2024.e33396

Clofazimine (CLF) is a riminophenazine derivative and a new therapeutic option with high efficacy for patients with rifampicin-resistant tuberculosis (TB). The blood levels of CLF are low and suboptimal, so therapeutic drug monitoring is required. Prior to this study, there were no molecular imprinting-based solid phase extraction (SPE) sorbents that could be used to determine the blood CLF levels. Hence, we prepared a magnetic molecularly imprinted polymer (MMIPs) to capture CLF. We employed computational selection of a functional monomer and crosslinker and confirmed these selections based on the association constant (K a) and a Job plot. We synthesised MMIPs with two surface modifiers and characterized the polymers. Our computational analysis based on the bond energy revealed that methyl methacrylate (MMA) was the most suitable functional monomer at a CLF-to-MMA molar ratio of 1:4. Based on the bond energy, the most suitable crosslinker was trimethylolpropane trimethacrylate (TRIM) at a CLF-to-TRIM molar ratio of 1:1. We determined the K a of MMA and TRIM in different solvents. Isopropanol produced the highest K a. The Job plot showed that a template-to-MMA-to-TRIM molar ratio of 1:4:20 was optimal to synthesize imprinted polymer in isopropanol. We prepared MMIPs using two different modifiers, namely aminopropyltrimethoxysilane (APTES) and oleic acid (OA), using the ratio determined from the Job plot. Physical characteristic tests carried out using FT-IR, SEM-EDS, PSA, BET and VSM, showed that the synthesis was success with a spherical and uniform agglomeration of particles, also a flat surface with many holes with a particle size of MMIP-APTES and MMIP-OA respectively 0.14 μm and 0.28 μm, showed a surface area for MMIP-APTES is 2874.51 m2/g and MMIP-OA 2913.07 m2/g, exhibiting superparamagnetic properties with a saturation magnetization value of MMIP-APTES 21.1 emu/g-1 and MMIP-OA 49.9 emu/g-1. Adsorption capacity result showed that MMIP-OA fits well with the Langmuir model, while MMIP-APTES fits better with the Freundlich. Application of MMIP-SPE (Magnetic Molecular Imprinted Polymer-Solid Phase Extraction) APTES resulted 92.3 ± 6.1 % and MMIP-SPE-OA 51.5 ± 8.1 % for recovering CLF in blood. The result of selectivity test also showed that MMIP-SPE-APTES is better than MMIP-SPE-OA and selectively recover CLF from human blood plasma existed together with other TB-Drugs. The study result shows that MMIPs with APTES modification can be used for CLF determination in human blood plasma.
Fulltext Optimisation of fermentation conditions for the production of gamma-aminobutyric acid (GABA)-rich soy sauce

Shin Yee C, Ilham Z, Cheng A, Abd Rahim MH, Hajar-Azhari S, Yuswan MH, et al.

Heliyon, 2024 Jul 15;10(13):e33147.
PMID: 39040394 DOI: 10.1016/j.heliyon.2024.e33147

This study addresses the challenge of enhancing gamma-aminobutyric acid (GABA) content in soy sauce through optimized fermentation condition. Using a multiple starter culture, consisting of Aspergillus oryzae strain NSK, Bacillus cereus strain KBC and Tetragenococcus halophilus strain KBC, the incubation conditions including the percentage of bacterial inoculum (10, 15 and 20 %), pH (3, 5 and 7) and agitation speed (100, 150 and 200 rpm) were optimized through Response Surface Methodology (RSM). Under the optimal conditions (20 % inoculum, pH 7 and stirring at 100 rpm), the multiple starter culture generated 128.69 mg/L of GABA after 7 days and produced 239.08 mg/L of GABA after 4 weeks of fermentation, which is 36 % higher than under non-optimized conditions (153.48 mg/L). Furthermore, sensory analysis revealed high consumer acceptance of the fermented soy sauce than the control (soy sauce without any treatment and additional bacteria) and commercial soy sauce. Consumers indicated that the starter culture offered an improved umami taste and reduced bitter, sour and salty flavours compared to the commercial product. Under optimal fermentation conditions determined by RSM statistical analysis, the multiple starter culture is able to produce high levels of GABA and is more likely to be accepted by consumers. The findings of this research have the potential to impact the food sector by offering a functional soy sauce with added health benefits and also being well-received by consumers.
Fulltext Dataset of youth and middle-aged unemployment in households in Kwara State, Nigeria

Ojuolape AM, Mohd S, Benjamin OO

Data Brief, 2024 Aug;55:110638.
PMID: 39040554 DOI: 10.1016/j.dib.2024.110638

This article shares the findings from survey conducted between November 2021 and April 2022 across the three senatorial districts of Kwara state, Nigeria. The survey was carried out across 1120 households with information on the head of households, individuals in the households, household demographic characteristics, education, work experience, household youth and middle-aged employment status. The survey included both open-ended and close-ended questions, and the resulting data in excel format. The coding explanation/ interpretation is also depicted in a table. This data is valuable for research on employment, empowerment, community development and studies on United Nations Sustainable Development Goals, precisely goal eight (UNSDG8).
Empowering Futures: Intersecting Comprehensive Sexual Education for Children and Adolescents With Sustainable Development Goals

Mohammed Tohit NF, Haque M

Cureus, 2024 Jul;16(7):e65078.
PMID: 39040612 DOI: 10.7759/cureus.65078

This scoping review synthesizes the intersection of comprehensive sexual education (CSE) for children and adolescents with sustainable development goals (SDGs) to assess the potential for empowering future generations. Considering the global challenges in addressing sexual health, this review explores the potential role of CSE in contributing to the achievement of the SDGs, particularly in the context of empowering youth and ensuring their well-being. The review begins by providing a comprehensive overview of CSE, emphasizing its role in promoting informed decision-making, gender equality, and preventing sexual and reproductive health issues among young individuals. It then delves into the overarching framework of the SDGs, mainly focusing on goals related to health, education, gender equality, and sustainable development. The synthesis examines the alignment and potential synergies between CSE and various SDGs, highlighting how CSE can contribute to outcomes such as improved health and well-being, quality education, gender equality, and reduced inequalities. Furthermore, the review brings attention to potential challenges and barriers in implementing CSE programs in different cultural and socio-economic contexts. Ultimately, this scoping review provides a critical analysis of the intersection between CSE and the SDGs, offering insights into how the comprehensive education of children and adolescents in sexual and reproductive health can play a significant role in advancing sustainable development and empowering future generations worldwide.
Fulltext Molecular simulation of [P8883][Tf2N] ionic liquid decorated silica in 6FDA-ODA based mixed matrix membrane for enhanced CO2/CH4 separation

Darban MA, Lock SSM, Ilyas SU, Kang DY, Othman MHD, Yiin CL, et al.

RSC Adv, 2024 Jul 19;14(32):22894-22915.
PMID: 39040689 DOI: 10.1039/d4ra02851a

Mixed-matrix membranes (MMMs) have been reported to have considerable scope in gas separation applications because of their merged inherent strength of a durable polymer matrix and the exceptional performance capabilities of inorganic fillers. The selection of comparatively suitable polymers with fillers that can match each other and boost interfacial compatibility while ensuring uniform dispersion of filler within the polymer is still intensively demanding and is challenging at the experimental scale. Ionic liquids (ILs) are effective in promoting better dispersion and compatibility, leading to improved separation performance. A computational molecular simulation approach is employed in current work to design a hybrid membrane having Trioctapropyl phosphonium bis(trifluoromethylsulfonyl)imide [P8883][Tf2N] IL decorated silica as a filler and 4,4'-(hexafluoroisopropylidene)diphthalic anhydride-4,4'-oxydianiline (6FDA-ODA) polymer for carbon dioxide (CO2) separation from methane (CH4). Thermophysical and gas transport properties under pure and mixed gas condition (30, 50, and 70% CO2/CH4) within the MMMs with varying filler loadings (5, 10, and 15 wt% IL-silica) are examined via Grand Canonical Monte Carlo (GCMC) and Molecular Dynamics (MD) simulations. Membrane characteristics like glass transition temperature (T g), Fractional Free Volume (v f), X-Ray Diffraction (XRD), solubility, diffusivity, permeability, and selectivity for neat and IL-silica filled 6FDA-ODA are computed. The results show that the T g of the composite membrane with 5 wt% IL-silica is found to be considerably higher (with 305 °C) than that of the pure 6FDA-ODA polymer having 298 °C. A higher T g value highlights the effective dispersion and higher adhesion between the filler and polymer membrane. Additionally, CO2 permeability for 5 wt% IL-silica/6FDA-ODA MMM is significantly improved, measuring 319.0 barrer while maintaining a CO2/CH4 selectivity of 16.2. These values are 89% and 56% respectively, greater than the corresponding values of neat 6FDA-ODA membrane. Published data from the literature review is used to validate the findings and guarantee their reliability. The obtained results exhibited an error in the range of 0.7-9%. Hence, it is concluded from the study that molecular simulation can be used to design IL decorated silica incorporated within 6FDA-ODA matrix, which is able to boost the interfacial compatibility, with elevated CO2/CH4 selectivity and CO2 permeability.
Thermography Research in Diabetic Foot: Insights From a Scopus-Based Bibliometric Study

Zakaria SA, Low CL, Kow RY, Zakaria Mohamad Z, Abidin MR, Che Ahmad A, et al.

Cureus, 2024 Jun;16(6):e62858.
PMID: 39040789 DOI: 10.7759/cureus.62858

Diabetic foot problems are among the most debilitating complications of diabetes mellitus. These problems incur significant economic costs and reduce quality of life. The integration of thermography technology in the screening and management of diabetic foot problems has been proven to be successful in recent years. By detecting changes in temperature, thermography helps identify early infections and assists in patient monitoring. These early successes have inspired more research and publications in this field. To date, a comprehensive bibliometric analysis of thermography-related research on diabetic foot using the Scopus database has not been conducted. This bibliometric analysis aims to fill this gap by reviewing the Scopus database from its inception until 2023 to examine the literature on thermography-related research on diabetic foot. A total of 342 articles met the selection criteria and were included in this analysis. The number of articles in this field remained low until the 2010s, when there was a sudden surge of interest that prompted numerous publications. Authors from the USA contributed the highest number of articles and had the greatest scholarly impact in this field. Despite the major contribution from the USA, there were numerous collaborations between various countries, underscoring the importance of international collaboration in advancing research and exchanging knowledge.

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