Browse publications by year: 2024

  1. Zhao J, Zhao J, Yuan H, Gao Z
    Front Nutr, 2024;11:1501536.
    PMID: 39777071 DOI: 10.3389/fnut.2024.1501536
    BACKGROUND: Weight and body shape concerns have become increasingly common among adolescents. Chinese university students show a high risk of eating disorder behaviors. This study aims to analyze the moderating effect of BMI on the relationships between body shape, attitudes, subjective norms, and eating disorder behavioral intentions among Chinese female university students using the Theory of Reasoned Action (TRA) model.

    METHODS: A stratified random sample of 679 female Chinese university students (age, mean ± SD = 19.792 ± 1.007) participated in the study. The surveys comprised the Theory of Reasoned Action Questionnaire (TRA-Q) and the Body Shape Questionnaire (BS-Q) to assess their body shape concerns and behavioral intentions regarding eating disorders. Structural equation modeling was used to test the extended TRA model, with body shape as an additional predictor and BMI as a moderator.

    RESULTS: Body shape positively affected attitudes (β = 0.444, p 

  2. Loh KJ, Ng ALO, Chia YC, Lee WL, Mohan D, Renganathan E
    PMID: 39780913 DOI: 10.51866/cm.674
    As dementia has been declared a global health crisis by the World Health Organization, this perspective paper aims to shed light on the cuerent stata of dementia care in Malaysia. The paper firsc outlinea eeveral barriers to dementia care in Malaysia. The shortage of geriatric specialists hampers the accessibility of dementia caee avd resceerces. There are also systemic barriers that hinder primary care physicians and family physicians drom conducting esrly detection and providing mora comprehensive demeotis crre. The lack of dementia-focused community services and public education further compounds issues for people living with dementia (PLWD) and their informal caregivers (ICs). Consequently, ICs suffer mentally from caregiving demands, yet evidence-based psychosocial services to support them are scarce in Malaysia. This paper wraps up with recommendations aligning with Malaysia's national plans and policies to prepare the nation for current and future dementia care needs.
  3. Ahmad Hamid AH, Zawawi NSM, Ali MF, Aziz NA
    PMID: 39780914 DOI: 10.51866/cr.731
    This case report delves into facilitating return to work (RTW) in a working-age stroke survivor. The patient was a 42-year-old Malay man who experienced multifocal lacunar infarctions in April 2022. He demonstrated substantial motor function recovery but presented with subtle cognitive deficits impacting various domains. The report outlines the diagnostic process of cognitive assessments and discusses the patient's medical history and stroke-related factors. The management plan encompassed a multidisciplinary approach in primary care, which involved incorporating cognitive rehabilitation, discussing barriers and exploring the perception of the ability to RTW. This case underscores the intricacies of mild cognitive impairment in working-age stroke survivors and emphasises the need for tailored interventions in primary care to optimise cognitive recovery and enhance the overall quality of life.
  4. Amirthalingam SD, Nalliah S
    PMID: 39780915 DOI: 10.51866/rv.620
    Vulvodynia manifests as persistent vulvar pain, affecting both sexual well-being and overall quality of life. While the precise cause remains elusive, various multifaceted predisposing and precipitating factors have been identified. Neurobiological and psychosocial elements contribute to a better understanding of the management of this complex disorder. Initial evaluation with detailed history-taking and focused pelvic examination are essential to exclude organic diseases contributing to vulvar pain. Management strategies are based on clinical experience, including non-pharmacological approaches and cognitive behavioural therapy. Oral pain-blocking medications such as serotonin-norepinephrine reuptake inhibitors and gabapentin as well as topical treatments such as oestrogen, lidocaine and gabapentin may be considered. More robust evidence is required for pharmacological treatments. Referral to a multidisciplinary team may be required in a proportion of patients who do not respond to conventional treatment. This concise review highlights the contemporary understanding of vulvodynia and proposes a practical approach within primary care settings.
  5. Gayatri M, Fajarningtiyas DN
    PMID: 39780916 DOI: 10.51866/oa.519
    INTRODUCTION: The desire for no additional births may be used to estimate the demand for family planning. Couple education may influence contraceptive use. This study aimed to examine the relationship between education and contraceptive use among women who desire no more children in Indonesia.

    METHODS: A dataset from the 2017 Indonesia Demographic and Health Survey (IDHS) was used. The sample consisted of 17,938 women aged 15-49 years who desired no more children. Binary logistic regression was conducted for data analyses.

    RESULTS: Of the total respondents, 73% used contraceptive methods. Contraceptive use was strongly associated with secondary education among couples (odds ratio [OR] = 1.27; 95% confidence interval [CI] = 1.17-1.37) and the working status of the husband (OR=2.62; 95% CI=2.07-3.32). The respondents living in rural areas (OR=1.14; 95% CI= 1.06-1.23) and in the Java-Bali region (OR=1.36; 95% CI=1.27-1.46) were more likely to use contraceptive methods. However, the respondents aged more than 35 years were less likely to use contraceptives.

    CONCLUSION: Among women who desire no more children, contraceptive use is associated with educational level, age and place of residence. Educated women tend to be empowered and have better access to contraceptive services, improving their chances of using contraception. Healthcare providers and policymakers need to promote the use of contraceptive services including counselling for couples with a low educational level, urban women and women who live in the outer Java-Bali region.

  6. Mohamed AA, Hussin FR, Zainulabid UA, Mohamed Rose I, Periyasamy PR
    Cureus, 2024 Dec;16(12):e75367.
    PMID: 39781121 DOI: 10.7759/cureus.75367
    Epstein-Barr virus-associated smooth muscle tumors (EBV-SMTs) are a rare type of tumor occurring exclusively in immunocompromised patients in the setting of HIV/AIDS, post-organ transplant, and congenital immunodeficiency. These tumors require demonstration of EBV DNA on histopathologic studies in order to establish a diagnosis. The overall prognosis is good. The principle of treatment is re-establishing the host immunity, which includes antiretroviral therapy (ART) in HIV/AIDS patients and reducing immunosuppressive therapy in post-transplant patients. The role of surgery is well established when the tumor is causing a mass effect, whereas chemotherapy and radiotherapy have a limited role. Herein, we report a case of a multifocal EBV-SMT in a patient with HIV, treated successfully with standard ART along with diagnostic and therapeutic surgical resection.
  7. Majeed ABA, Ramasamy K, Tan MP, LIm SM, Hui KM, Tan AH, et al.
    Alzheimers Dement, 2024 Dec;20 Suppl 6(Suppl 6):e093563.
    PMID: 39782435 DOI: 10.1002/alz.093563
    BACKGROUND: Gut microbiota modulation of the brain function may present an opportunity to devise preventive or treatment strategies to manage impairments such as cognitive frailty (CF). This study aims to uncover the relationship between CF, gut microbiota, intestinal permeability and proteome.

    METHOD: A total of 137 fecal samples of the elderly were collected, and subjected to DNA analysis, and enzyme-linked immunosorbent assays (ELISA). Plasma samples were subjected to mass spectrometry proteomic analysis. The parameters of the subjects measured include functional reach test (FRT), handgrip strength (HGS), Visual Cognitive Assessment Test (VCAT), Montreal Cognitive Assessment (MoCA), timed up and go (TUG) and UCLA three-item loneliness scale (UCLA-3).

    RESULT: At the genus level, Alistipes which are potential drivers of dysbiosis, are significantly increased in CF subjects. Proteobacteria are also negatively linked to FRT, HGS, VCAT, and MoCA, but positively correlated to TUG and UCLA-3. Lactoferrin was upregulated in pre-frail subjects. The plasma apolipoprotein AI (Apo-AI) was upregulated 5 times in the CF subjects.

    CONCLUSION: These findings provide evidence for dietary intervention to alter gut microbiota that may modulate cognitive status.

    MeSH terms: Aged; Aged, 80 and over; Feces/microbiology; Feces/chemistry; Female; Humans; Male; Proteomics; Mild Cognitive Impairment; Dysbiosis
  8. Muthuraman A, Lim KG
    Alzheimers Dement, 2024 Dec;20 Suppl 6(Suppl 6):e088448.
    PMID: 39782647 DOI: 10.1002/alz.088448
    BACKGROUND: Senile dementia (SD) is a deteriorative organic brain disorder and it comprises Alzheimer's disease (AD) as a major variant. SD is shown impairment of mental capacities whereas AD is degeneration of neurons. According to World Health Organization (WHO) report; more than 55 million peoples have dementia and it is raising 10 million new cases every year. Further, it is expected to rise to 78 million in 2030 and triple by 2050. The glucose oxidase (E.C. 1.1.3.4; GOD) is an oxidoreductase enzyme and it oxidizes the β-d-glucose to d-glucono-δ-lactone and hydrogen peroxide (H2O2). In the neuronal system, GOD & H2O2 cause rapid fluctuations of glucose levels, insulin resistance, oxidative stress along with microglial activation and neurodegenerations. Besides, only a few medications were approved for the symptomatic relief of SD i.e., donepezil, galantamine, rivastigmine, and memantine. Moreover, the most efficient and potential agents are not discovered yet. Hence, the present study was designed to investigate the beta-carotene (BC) actions against the GOD-associated SD in diabetic rats.

    METHOD: Male Sprague Dawley rats were used for the induction of diabetes by intraperitoneal injection of nicotinamide (50 mg/kg and streptozotocin 50 mg/kg), and SD was induced by intracisternal administration of GOD (50 U/5 µl at 1 µL/minute). The BC (50 and 100 mg/kg; p.o.) and donepezil (1 mg/kg; p.o.) were administered for 15 consecutive days. The cognitive function was assessed by the Morris water maze test and biomarkers i.e., blood glucose & insulin; serum nitric oxide (NO); tissue acetylcholinesterase (AChE), galectin-1 (G1), NADPH oxidase (NOX4) activity & glucose transporter 1 (GLUT1) levels were evaluated.

    RESULT: GOD potentially changes the neurovascular unit in the brain which leads to a rise the insulin resistance (IR), NO, G1, & GLUT1 levels; and decreases the NOX4 activity. The GOD causes the potential cognitive dysfunctions. However, the treatment of BC attenuated the GOD-associated cognitive dysfunction and biomarker changes.

    CONCLUSION: The present results revealed that BC possesses the ameliorative potential against GOD-induced neurotoxicity and SD due to its anti-oxidative, anti-cholinesterase, reduction of IR, prevention of microglial activation, and enhancement of the glucose update actions.

    MeSH terms: Alzheimer Disease/drug therapy; Animals; Diabetes Mellitus, Experimental/drug therapy; Male; Rats, Sprague-Dawley*; Oxidative Stress/drug effects; beta Carotene/pharmacology; Rats
  9. Cao S, Liu J, Geok SK, Sun H, Wang X
    PLoS One, 2024;19(12):e0306815.
    PMID: 39739709 DOI: 10.1371/journal.pone.0306815
    OBJECTIVE: The detrimental effects of mental fatigue (MF) have been established in sports, such as soccer, volleyball, and basketball. Mindfulness interventions are considered a promising method to help players counteract MF, but whether it could improve basketball tactical performance after MF in competition is not clear. This study aims to investigate the effect of brief mindfulness intervention on basketball tactical performance under MF.

    METHOD: This study employed a cluster randomized controlled trial (cRCT) design. It involved 54 male basketball players aged 18 to 24 from three universities. The participants were randomly assigned to one of three groups: control group (CG), mental fatigue group (MFG), and mental fatigue-mindfulness group (MF-MG). Players in the MFG and MF-MG underwent a 30-minute Stroop task to induce MF. Subsequently, players in the MF-MG engaged in a 30-minute audio mindfulness intervention. Basketball tactical performance was assessed in the small side games (SSG).

    RESULTS: There were no significant differences in total tactics observed across groups and over time. However, when examining specific tactical sub-variables, significant differences were found in ball reversal, dribble penetration into the key area, and off-ball screen between the CG and MFG in the post-test. Furthermore, significant differences were noted in ball reversal, dribble penetration into the key area, on-ball screen, and off-ball screen between the MFG and MF-MG in the post-test.

    CONCLUSION: The basketball tactical performances, particularly in areas such as ball reversal, dribble penetration into the key area, on-ball screen, and off-ball screen, were negatively impacted by MF. Notably, the brief mindfulness intervention effectively restored these performance aspects. This suggests that coaches and trainers should place increased emphasis on players' mental well-being and consider incorporating brief mindfulness interventions into their training programs. More studies that investigate mindfulness intervention on the comprehensive aspects of basketball performance should be focused on in the future.

    MeSH terms: Adolescent; Adult; Mental Fatigue*; Humans; Male; Young Adult
  10. Amer H, Kampan NC, Itsiopoulos C, Flanagan KL, Scott CL, Kartikasari AER, et al.
    Cancers (Basel), 2024 Dec 16;16(24).
    PMID: 39766086 DOI: 10.3390/cancers16244187
    Despite significant advances in treatments, ovarian cancer (OC) remains one of the most prevalent and lethal gynecological cancers in women. The frequent detection at the advanced stages has contributed to low survival rates, resistance to various treatments, and disease recurrence. Thus, a more effective approach is warranted to combat OC. The cytokine Interleukin-6 (IL6) has been implicated in various stages of OC development. High IL6 levels are also correlated with a lower survival rate in OC patients. In this current review, we summarized the pivotal roles of IL6 in OC, including the initiation, development, invasion, metastasis, and drug resistance mechanisms. This article systematically highlights how targeting IL6 improves OC outcomes by altering various cancer processes and reports the ongoing clinical trials that would further shape the IL6-based targeted therapies. This review also suggests how combining IL6-targeted therapies with other therapeutic strategies could further enhance their efficacy to combat OC.
  11. Elizar E, Muharar R, Zulkifley MA
    Diagnostics (Basel), 2024 Dec 14;14(24).
    PMID: 39767181 DOI: 10.3390/diagnostics14242820
    BACKGROUND: Cardiac magnetic resonance imaging (MRI) plays a crucial role in monitoring disease progression and evaluating the effectiveness of treatment interventions. Cardiac MRI allows medical practitioners to assess cardiac function accurately by providing comprehensive and quantitative information about the structure and function, hence making it an indispensable tool for monitoring the disease and treatment response. Deep learning-based segmentation enables the precise delineation of cardiac structures including the myocardium, right ventricle, and left ventricle. The accurate segmentation of these structures helps in the diagnosis of heart failure, cardiac functional response to therapies, and understanding the state of the heart functions after treatment.

    OBJECTIVES: The objective of this study is to develop a multiscale deep learning model to segment cardiac organs based on MRI imaging data. Good segmentation performance is difficult to achieve due to the complex nature of the cardiac structure, which includes a variety of chambers, arteries, and tissues. Furthermore, the human heart is also constantly beating, leading to motion artifacts that reduce image clarity and consistency. As a result, a multiscale method is explored to overcome various challenges in segmenting cardiac MRI images.

    METHODS: This paper proposes DeSPPNet, a multiscale-based deep learning network. Its foundation follows encoder-decoder pair architecture that utilizes the Spatial Pyramid Pooling (SPP) layer to improve the performance of cardiac semantic segmentation. The SPP layer is designed to pool features from densely convolutional layers at different scales or sizes, which will be combined to maintain a set of spatial information. By processing features at different spatial resolutions, the multiscale densely connected layer in the form of the Pyramid Pooling Dense Module (PPDM) helps the network to capture both local and global context, preserving finer details of the cardiac structure while also capturing the broader context required to accurately segment larger cardiac structures. The PPDM is incorporated into the deeper layer of the encoder section of the deep learning network to allow it to recognize complex semantic features.

    RESULTS: An analysis of multiple PPDM placement scenarios and structural variations revealed that the 3-path PPDM, positioned at the encoder layer 5, yielded optimal segmentation performance, achieving dice, intersection over union (IoU), and accuracy scores of 0.859, 0.800, and 0.993, respectively.

    CONCLUSIONS: Different PPDM configurations produce a different effect on the network; as such, a shallower layer placement, like encoder layer 4, retains more spatial data that need more parallel paths to gather the optimal set of multiscale features. In contrast, deeper layers contain more informative features but at a lower spatial resolution, which reduces the number of parallel paths required to provide optimal multiscale context.

  12. Siau CS, Zhang J, Chen WS, Mohd Saat NZ, Lew B, Ma X, et al.
    Behav Sci (Basel), 2024 Dec 02;14(12).
    PMID: 39767298 DOI: 10.3390/bs14121157
    The Psychological Strain Scale (PSS) has been widely used in various populations to assess the risks of suicidality and mental disorders. The current study aims to shorten the original 40-item scale and test its psychometric properties. Data were derived from three samples in China: (1) undergraduate students (n = 10,742), (2) non-medical office employees (n = 1044), and (3) hospital workers (n = 949). A questionnaire was administered to the samples at about the same time of the year 2017. Data were randomly divided into Sub-study 1 (70% of the sample) and Sub-study 2 (30% of the sample). In Sub-study 1, principal component analyses were run and five items with the highest factor loadings within each subscale were retained, resulting in a 20-item PSS scale. Cronbach's α estimates were above 0.70. In Sub-study 2, confirmatory factor analyses for the three samples revealed RMSEA values below 0.10, PNFI values below 0.50 for all samples, and CFI, TLI and NFI values above 0.90 for the student and non-medical office employee samples, but the hospital worker sample had a TLI of 0.88. The PSS-20 and its subscales were significantly associated with past-year suicidal ideation. The PSS-20 has acceptable psychometric properties but requires further testing in university students and hospital workers.
  13. Xu X, Hanafi Z, Razak NA
    Behav Sci (Basel), 2024 Dec 15;14(12).
    PMID: 39767344 DOI: 10.3390/bs14121203
    Parental behavioral control is one of the most common parenting practices employed by parents in China. It is necessary to identify this practice and how it may be influenced by adolescents' and parents' sociodemographic factors in order to develop family intervention strategies. The present study examined whether fathers' and mothers' behavioral control was related to adolescents' gender, age, and sibling status and parents' age, education level, and income. A sample of 916 adolescents, aged 11-15 years, reported their fathers' and mothers' parental behavioral control. In terms of adolescents' gender, boys perceived higher level of fathers' behavioral control compared to girls. Regarding parents' sociodemographic factors, the level of mothers' behavioral control was higher compared to that of fathers. Mothers' education levels were reported to have a negative relationship with maternal behavioral control. Regarding adolescents' gender differences, there was a positive relationship between fathers' educational levels and behavioral control in adolescent boys. However, there was a positive relationship between fathers' monthly income and fathers' behavioral control in adolescent girls rather than boys. In addition, there was a negative relationship between mothers' age and behavioral control in adolescent girls but not in boys. These findings add to the literature on Chinese mothers' and fathers' parenting and offer practical implications for Chinese family interventions.
  14. Thangasparan S, Kamisah Y, Ugusman A, Mohamad Anuar NN, Ibrahim N'
    Int J Mol Sci, 2024 Dec 11;25(24).
    PMID: 39769058 DOI: 10.3390/ijms252413292
    Cardiovascular diseases (CVD) are the number one cause of death worldwide, with atherosclerosis, which is the formation of fatty plaques in the arteries, being the most common underlying cause. The activation of inflammatory events and endothelium dysfunction are crucial for the development and pathophysiology of atherosclerosis. Elevated circulating levels of low-density lipoprotein (LDL) have been associated with severity of atherosclerosis. LDL can undergo oxidative modifications, resulting in oxidised LDL (oxLDL). OxLDL has been found to have antigenic potential and contribute significantly to atherosclerosis-associated inflammation by activating innate and adaptive immunity. Various inflammatory stimuli such as interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-α) and intercellular adhesion molecule 1 (ICAM-1) play major roles in atherosclerosis. To date, studies have provided valuable insights into the role of oxLDL in the development of atherosclerosis. However, there remains a gap in understanding the specific pathways involved in this process. This review aims to provide and discuss the mechanisms by which oxLDL modulates signalling pathways that cause cardiovascular diseases by providing in vitro and in vivo experimental evidence. Its critical role in triggering and sustaining endothelial dysfunction highlights its potential as a therapeutic target. Advancing the understanding of its atherogenic role and associated signalling pathways could pave the way for novel targeted therapeutic strategies to combat atherosclerosis more effectively.
    MeSH terms: Animals; Humans; Inflammation/metabolism; Signal Transduction
  15. Zhu C, Yang H, Zhu W, Jiang Q, Dong Z, Wang L
    Int J Mol Sci, 2024 Dec 13;25(24).
    PMID: 39769137 DOI: 10.3390/ijms252413372
    Cold stress during overwintering is considered a bottleneck problem limiting the development of the red tilapia (Oreochromis spp.) industry, and the regulation mechanism is currently not well understood. In this study, the fish (initial weight: 72.71 ± 1.32 g) were divided into the cold stress group (cold) and the control (normal) group. In the control group, the water temperature was maintained at 20 °C, which is basically consistent with the overwintering water temperature in greenhouses of local areas. In the cold group, the water temperature decreased from 20 °C to 8 °C by 2 °C per day during the experiment. At the end of the experiment, the levels of fish serum urea nitrogen, glucose, norepinephrine, alkaline phosphatase, total bilirubin, and total cholesterol in the cold group changed significantly compared with that in the control group (P < 0.05). Then transcriptome sequencing and LC-MS metabolome of brain tissue were further employed to obtain the mRNA and metabolite datasets. We found that the FoxO signaling pathway and ABC transporters played an important role by transcriptome-metabolome association analysis. In the FoxO signaling pathway, the differentially expressed genes were related to cell cycle regulation, apoptosis and immune-regulation, and oxidative stress resistance and DNA repair. In the ABC transporters pathway, the ATP-binding cassette (ABC) subfamily abca, abcb, and abcc gene expression levels, and the deoxycytidine, L-lysine, L-glutamic acid, L-threonine, ornithine, and uridine metabolite contents changed. Our results suggested that the cold stress may promote apoptosis through regulation of the FoxO signaling pathway. The ABC transporters may respond to cold stress by regulating amino acid metabolism. The results provided a comprehensive understanding of fish cold stress during overwintering, which will facilitate the breeding of new cold-resistant varieties of red tilapia in the future.
    MeSH terms: Animals; Gene Expression Regulation; Seasons; Signal Transduction; Gene Expression Profiling; Metabolome*; Cold-Shock Response*; Transcriptome*
  16. Abdelsalam SS, Zahid MA, Ghanem SK, Khan A, Parray A, Agouni A
    Int J Mol Sci, 2024 Dec 16;25(24).
    PMID: 39769227 DOI: 10.3390/ijms252413463
    Sestrin2 (SESN2) is a stress-inducible protein known for its cytoprotective functions, but its role in diabetic vascular complications remains unclear. This study investigated the impact of SESN2 on methylglyoxal (MGO)-induced endothelial-mesenchymal transition (EndMT). Human endothelial cells were transfected with SESN2 siRNA duplexes to silence SESN2 expression, followed by MGO treatment. SESN2 knockdown significantly exacerbated MGO-induced oxidative stress, as evidenced by the reduced expression of antioxidant markers. Furthermore, SESN2 silencing enhanced the inflammatory response to MGO, demonstrated by the increased levels of pro-inflammatory cytokines. Notably, SESN2 deficiency promoted EndMT, a key process in diabetes-induced cardiovascular complications, as shown by the increased expression of mesenchymal markers and the decreased expression of endothelial markers. These findings suggest that SESN2 plays a critical protective role in endothelial cells against MGO-induced damage. The study provides novel insights into the molecular mechanisms underlying diabetic cardiovascular complications and identifies SESN2 as a potential therapeutic target for preventing endothelial dysfunction in diabetes. Our results indicate that SESN2 downregulation may contribute to the pathogenesis of diabetic vascular complications by promoting EndMT, increased oxidative stress, and inflammation.
    MeSH terms: Humans; Inflammation/metabolism; Inflammation/pathology; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Oxidative Stress*; Endothelial Cells/drug effects; Endothelial Cells/metabolism; Epithelial-Mesenchymal Transition/drug effects; Epithelial-Mesenchymal Transition/genetics
  17. Shahar FS, Sultan MTH, Grzejda R, Łukaszewicz A, Oksiuta Z, Krishnamoorthy RR
    Materials (Basel), 2024 Dec 10;17(24).
    PMID: 39769644 DOI: 10.3390/ma17246045
    Natural composites are emerging as promising alternative materials for 3D printing in biomedical applications due to their biocompatibility, sustainability, and unique mechanical properties. The use of natural composites offers several advantages, including reduced environmental impact, enhanced biodegradability, and improved tissue compatibility. These materials can be processed into filaments or resins suitable for various 3D printing techniques, such as fused deposition modeling (FDM). Natural composites also exhibit inherent antibacterial properties, making them particularly suitable for applications in tissue engineering, drug delivery systems, and biomedical implants. This review explores the potential of utilizing natural composites in additive manufacturing for biomedical purposes, discussing the historical development of 3D printing techniques; the types of manufacturing methods; and the optimization of material compatibility, printability, and mechanical properties to fully realize the potential of using natural fibers in 3D printing for biomedical applications.
  18. Wan J, Zhao ZY, Wang C, Jiang CX, Tong YP, Zang Y, et al.
    Molecules, 2024 Dec 18;29(24).
    PMID: 39770069 DOI: 10.3390/molecules29245980
    A comprehensive phytochemical investigation of the twigs/leaves and flower buds of Kolkwitzia amabilis, a rare deciduous shrub native to China, led to the isolation of 39 structurally diverse compounds. These compounds include 11 iridoid glycosides (1-4 and 7-13), 20 triterpenoids (5, 6, and 14-31), and 8 phenylpropanoids (32-39). Among these, amabiliosides A (1) and B (2) represent previously undescribed bis-iridoid glycosides, while amabiliosides C (3) and D (4) feature a unique bis-iridoid-monoterpenoid indole alkaloid scaffold with a tetrahydro-β-carboline-5-carboxylic acid moiety. Amabiliacids A (5) and B (6) are 24-nor-ursane-type triterpenoids characterized by an uncommon ∆11,13(18) transannular double bond. Their chemical structures and absolute configurations were elucidated through spectroscopic data and electronic circular dichroism analyses. Compound 2 exhibited a moderate inhibitory effect against acetyl CoA carboxylase 1 (ACC1), with an IC50 value of 9.6 μM. Lonicejaposide C (8), 3β-O-trans-caffeoyl-olean-12-en-28-oic acid (29), and (23E)-coumaroylhederagenin (31) showed notable inhibitory effects on ATP-citrate lyase (ACL), with IC50 values of 3.6, 1.6, and 4.7 μM, respectively. Additionally, 3β-acetyl-ursolic acid (17) demonstrated dual inhibitory activity against both ACC1 and ACL, with IC50 values of 10.3 and 2.0 μM, respectively. The interactions of the active compounds with ACC1 and ACL enzymes were examined through molecular docking studies. From a chemotaxonomic perspective, the isolation of bis-iridoid glycosides in this study may aid in clarifying the taxonomic relationship between the genera Kolkwitzia and Lonicera within the Caprifoliaceae family. These findings highlight the importance of conserving plant species with unique and diverse secondary metabolites, which could serve as potential sources of new therapeutic agents for treating ACC1/ACL-associated diseases.
    MeSH terms: Enzyme Inhibitors/isolation & purification; Enzyme Inhibitors/pharmacology; Enzyme Inhibitors/chemistry; Plant Extracts/pharmacology; Plant Extracts/chemistry; Molecular Structure; Molecular Docking Simulation
  19. Goh KM, Tan ESS, Lim CSY, Tan PY, Biswas S, Lew LA, et al.
    Nutrients, 2024 Dec 18;16(24).
    PMID: 39770987 DOI: 10.3390/nu16244366
    BACKGROUND/OBJECTIVES: Dry eye disease (DED) significantly impairs quality of life, affecting physical, social, and psychological well-being, as well as reducing workplace productivity. While lutein and zeaxanthin supplements have been shown to improve ocular health, existing research often overlooks the efficacy of lower dosages and shorter durations of supplementation. This study investigated the effects of combined supplementation with lutein, zeaxanthin, and elderberries in 110 voluntary participants through a randomized controlled trial.

    METHODS: Participants took 6 mg of lutein and 1 mg of zeaxanthin, along with 100 mg elderberry extract once daily for a duration of 20 days. Ocular health was assessed using the Ocular Surface Disease Index (OSDI), while immune status was evaluated with the Immune Status Questionnaire (ISQ).

    RESULTS: Results showed that combined supplementation significantly (p < 0.05) reduced the OSDI scores in the intervention group from 38.15 ± 11.14 to 18.26 ± 5.57, reflecting a 52.2% reduction. A similar trend was observed with the Visual Analog Scale (VAS), indicating significant (p < 0.05) improvement from 5.31 ± 1.62 to 6.73 ± 1.74, equivalent to a 26.7% improvement. Although the intervention group showed a 15.9% improvement in ISQ scores by the study's end, this was not significantly different from the placebo group, suggesting that higher dosages or longer durations may be needed to observe a meaningful effect. Additionally, findings from the Food Frequency Questionnaire revealed that the average dietary intake of lutein and zeaxanthin among participants was only 663.49 µg, equating to just 5.5% of the suggested optimal daily intake. This low consumption is concerning, as it is inversely correlated with the risk of ocular diseases.

    CONCLUSIONS: Collectively, these findings support the use of combined supplementation as an adjuvant approach to improving ocular health.

    MeSH terms: Adult; Aged; Female; Humans; Male; Middle Aged; Plant Extracts/administration & dosage; Quality of Life; Surveys and Questionnaires; Dietary Supplements*
  20. Sulieman AME, Idriss H, Alshammari M, Almuzaini NAM, Ibrahim NA, Dahab M, et al.
    Plants (Basel), 2024 Dec 13;13(24).
    PMID: 39771189 DOI: 10.3390/plants13243491
    The arid mountainous region of Hail in Saudi Arabia has a variety of desert vegetation, some of which are conventionally used in Bedouin traditional medicine. These plants need scientific examination. This research seeks to examine Blepharis ciliaris using a thorough multi-analytical methodology that includes antibacterial and antioxidant assessments as well as computational modeling. GC-MS analysis of the methanolic extract revealed 17 organic compounds, including pentadecanoic acid, ethyl methyl ester (2.63%); hexadecanoic acid, methyl ester (1.00%); 9,12-octadecadienoic acid (Z,Z)-, methyl ester (2.74%); 9-octadecenoic acid, methyl ester (E) (2.78%); octadecanoic acid (5.88%); 9-tetradecenoic acid (Z) (3.22%); and undec-10-enoic acid, undec-2-n-1-yl ester (5.67%). The DPPH test evaluated antioxidant activity, revealing a notable increase with higher concentrations of the methanolic extract, achieving maximum inhibition of 81.54% at 1000 µg/mL. The methanolic extract exhibited moderate antibacterial activity, with average inhibition zones of 10.33 ± 1.53 mm, 13.33 ± 1.53 mm, 10.67 ± 1.53 mm, and 10.00 ± 2.00 mm against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Serratia marcescens, respectively, as determined by the disk diffusion method. The minimum inhibitory concentration (MIC) values were 500 µg/mL for S. aureus and B. subtilis, whereas E. coli and S. marcescens showed susceptibility at 1000 µg/mL. Computational simulations were employed to assess the toxicity, drug-likeness, and ADMET profiles of compounds derived from Blepharis ciliaris. Thirteen bioactive compounds were assessed in silico against Staphylococcus aureus sortase A (PDB: 1T2O), Bacillus subtilis BsFabHb (PDB: 8VDB), Escherichia coli LPS assembly protein (LptD) (PDB: 4RHB), and a modeled Serratia marcescens outer-membrane protein TolC, focusing on cell wall and membrane structures. Compound 3, (+)-Ascorbic acid 2,6-dihexadecanoate, shown significant binding affinities to B. subtilis BsFabHb, E. coli LPS assembly protein, and S. marcescens TolC.
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